CN2567225Y - Device for maitaining high water activity in solid state fermentation process - Google Patents
Device for maitaining high water activity in solid state fermentation process Download PDFInfo
- Publication number
- CN2567225Y CN2567225Y CN 02245657 CN02245657U CN2567225Y CN 2567225 Y CN2567225 Y CN 2567225Y CN 02245657 CN02245657 CN 02245657 CN 02245657 U CN02245657 U CN 02245657U CN 2567225 Y CN2567225 Y CN 2567225Y
- Authority
- CN
- China
- Prior art keywords
- solid
- solid state
- state fermentation
- ultrasonic atomizer
- water activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/16—Solid state fermenters, e.g. for koji production
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
Abstract
The utility model relates to a device for maintaining high water activity in a solid state fermentation process, which comprises a solid fermentation container, and the container wall of the solid state fermentation container is provided with an air inlet pipe which is provided with an air inlet electromagnetic valve, and an air exhausting port. A water absorption material frame is installed in the solid state fermentation container, and ultrasonic atomizers are arranged on the middle parts of both ends of the solid state fermentation container. A power supply for the ultrasonic atomizers is electrically connected with the ultrasonic atomizers through a single chip hygrostat. In a fermentation process, according to the humidity requirements by different micro organisms or different fermentation stages, the ultrasonic atomizers are opened by the on-line control of the single chip hygrostat. In a solid state fermentation process, the utility model flexibly control the activity of water or the humidity in the solid state fermentation container, solves the problems that in a traditional watering or humdification method, sterile operation is difficult to reach, and the contamination by impure bacteria is easy to happen. The device has the advantages of simple structure and convenient operation.
Description
Technical field
The utility model relates to a kind of device of the usefulness of fermenting, particularly a kind of device that is used for solid ferment process maintenance high water activity.
Background technology
Modern fermentation industry is the main part of producing with industrialized mode in the contemporary biotechnology industry, one of important symbol that its fermentation technique level is a national biotechnology industry developmental level.As everyone knows, microbial fermentation processes has two classes: liquid submerged fermentation and solid state fermentation.The large-scale industrial production of penicillin in 1945 has been started liquid submerged fermentation technology and modern fermentation industry.But the productivity ratio liquid submerged fermentation of many modern biological products solid state fermentations is much higher, on the other hand, the bulk fermentation waste water that liquid submerged fermentation produces, ventilation and churned mechanically high power consumption, become the obstacle that liquid submerged fermentation further develops again, force it to develop to high density, high viscosity direction.Yet high density, the full-bodied limit are exactly solid state fermentation.Solid-state fermentation technology is that the human use microorganism benefits one of the most ancient technology of oneself.Before several thousand, China just utilizes this technology wine brewing and makes various seasonings.So far it is still the main mode of producing traditional food and beverage.Solid state fermentation can the production zymin, single cell protein, organic acid, alcohol and biotic pesticide etc.Solid state fermentation is also to have obtained using widely at the processing of biological transformation of energy, solid-state castoff, the aspects such as production of meta-bolites at present.Characteristics such as fermentation costs is low because solid state fermentation has, and cost of material is cheap, does not have " three wastes " discharging, and environmental pollution is little, and technological process is simple at present, progressively obtain the attention of countries in the world.
But, because the characteristics of solid state fermentation maximum do not have free-water exactly, thereby the variation of substrate moisture content, will inevitably produce material impact to microbial growth and metabolic capacity.Water activity (aw) expression microorganism is to the accessibility of water, the water activity of matrix be influence microorganism can be on substrate the key factor of normal growth, it is relevant with the water content of substrate.And move in the thermal process at solid state fermentation, the moisture evaporation cooling accounts for and moves 80% of heat, along with the increase that moves hot speed, will inevitably cause reducing of soil humidity, changes the size of aw.Therefore, how keeping higher water activity in the solid ferment process is the bottle footpath problem of solid state fermentation widespread use.Document WO 9418306A1 introduces among the BO1F/30 to some extent.At present, directly spray water or the direct method of humidifying air, exist and be difficult to accomplish aseptic technique, easily dye assorted bacterium, to shortcomings such as the solid medium humidification are inhomogeneous at solid ferment process.
Summary of the invention
One of the purpose of this utility model is be difficult for to keep in solid ferment process than high water activity with to shortcomings such as the solid medium humidification are inhomogeneous to overcome traditional humidification water replanishing device.Two of the purpose of this utility model is integrated application ultrasonic atomizatio humidifications, with water-absorbing material evaporation humidification process, or fermentor tank pressure pulsation spraying humidification, and in solid-state fermenter, finish whole humidification, in order to overcome the shortcoming of in solid ferment process, easily dying assorted bacterium; Thereby provide a kind of and can get final product flexible humidification degree, reach the device that the solid medium humidification is used for uniformly solid ferment process maintenance high water activity again according to the requirement difference of microorganism to water activity.
The purpose of this utility model is achieved in that
A kind of device that is used for solid ferment process maintenance high water activity that the utility model provides, comprising: a solid-state fermenter has the inlet pipe and the venting port that are installed into the pneumoelectric magnet valve, humidity sensor and micro-chip humidity controller on its tank skin; It is characterized in that also comprising: water-absorbing material frame, fog-spray nozzle, ultrasonic atomizer; Wherein evenly be divided into trilaminar water-absorbing material frame and be installed in the solid-state fermenter, ultrasonic atomizer is at the middle part at solid-state fermenter two ends; The fog-spray nozzle of this ultrasonic atomizer is installed at the end of solid-state fermenter, and the ultrasonic atomizer power supply is electrically connected with ultrasonic atomizer by micro-chip moisture content measurement controller.
Described solid-state fermenter is an airtight withstand voltage 0.3Mpa, horizontal low pressure vessel.
The stainless steel reel that described water-absorbing material frame is 0.45m * 0.55m * 0.02m, placement steam puffed stalk or paper pulp are used.
Described ultrasonic atomizer has 3 or 3 above fog-spray nozzles.
Advantage of the present utility model is:
Of the present utility model be used for device that solid ferment process keeps high water activity have simple in structure, be easy to promote large-scale industrialization production.
Device of the present utility model is based on the characteristics of solid ferment process, and the evaporation of integrated application water-absorbing material humidification, ultrasonic atomizatio humidifying technology and fermentor tank pressure pulsation spraying humidification enlarge evaporation area and humidification, keep water balance.Can use device of the present utility model according to the different microorganisms or the solid state fermentation in different fermentations stage, use the micro-chip humidity controller and control water activity or humidity in the solid-state fermenter process flexibly, thereby reach even the solid medium humidification.The humidification of fermenting process is finished in solid-state fermenter in addition, has avoided tradition to add water or air-humidification method is difficult to accomplish aseptic technique, easily dyes the shortcoming of assorted bacterium.
Description of drawings
Fig. 1 apparatus structure synoptic diagram of the present utility model
The drawing explanation:
1 water-absorbing material frame; 2 fog-spray nozzles; 3 ultrasonic atomizers;
4 solid-state fermenters, 5 humidity sensors; 6 micro-chip humidity controllers;
7 air inlet magnetic valves; 8 sterile air inlet pipe; 9 solid fermentation materials;
Embodiment
Embodiment 1
Make the device of a maintenance high water activity in solid ferment process of the present utility model by Fig. 1
Comprise: airtight, withstand voltage 0.3Mpa, a horizontal low pressure vessel is as solid-state fermenter 4, install one in this solid-state fermenter 4 and evenly be divided into trilaminar water-absorbing material frame 1, this water-absorbing material frame 1 is the stainless steel reel of 0.45m * 0.55m * 0.02m, placement steam puffed stalk or paper pulp 9 usefulness; Installation one has the inlet pipe 10 of 3 air inlet magnetic valves 7 on its tank skin, and a venting port 11 is installed on its tank skin, and humidity sensor 5, micro-chip humidity controller 6, fog-spray nozzle 2 and ultrasonic atomizer 3 all adopt commercially available; Ultrasonic atomizer 3 is at the middle part at solid-state fermenter 4 two ends; This ultrasonic atomizer 3 has 8 fog-spray nozzles 2, and is installed in respectively uniformly at the end of solid-state fermenter 4, and the ultrasonic atomizer power supply is electrically connected with commercially available ultrasonic atomizer 3 by a common micro-chip moisture content measurement controller 6.
Embodiment 2
On the device of embodiment 1, also be included in solid-state fermenter 4 common pressure pulsation device is installed, during the fermentation, solid-state fermenter is carried out timing pressure pulsation, in the process of boosting, sterile air from the fog-spray nozzle ejection, makes a jar interior condensate water atomizing humidification by sterile air inlet pipe 8;
Embodiment 3
In the device that embodiment 1 makes, utilize the method for maintenance high water activity in solid ferment process of the present utility model to carry out the solid state fermentation of steam puffed stalk or paper pulp, process is as follows:
(1) put into strong substrate steam puffed stalk of suction force or paper pulp 1000 grams in the water-absorbing material frame 1 in solid-state fermenter 4, under 0.1Mpa steam, sterilizing air 30 minutes;
(2) after the cooling, place postvaccinal cultivation material gram on the fermentation frame, controlled temperature carries out cultivation and fermentation routinely;
(3) during the fermentation, according to different microorganisms or different fermentations stage to humidity requirement, by the unlatching of micro-chip moisture content measurement controller on-line Control ultrasonic atomizer.
Claims (3)
1. one kind is used for the device that solid ferment process keeps high water activity, and it is characterized in that comprising: a solid-state fermenter 4 has the inlet pipe 10 and the venting port 11 that are installed into pneumoelectric magnet valve 7, humidity sensor 5 and micro-chip humidity controller 6 on its tank skin; It is characterized in that also comprising: water-absorbing material frame 1, fog-spray nozzle 2, ultrasonic atomizer 3; Wherein evenly be divided into trilaminar water-absorbing material frame 1 and be installed in the solid-state fermenter 4, ultrasonic atomizer 3 is at the middle part at solid-state fermenter 4 two ends; This ultrasonic atomizer 3 has a plurality of fog-spray nozzles 2, and is installed at the end of solid-state fermenter 4, and ultrasonic atomizer power supply 6 is electrically connected with ultrasonic atomizer by micro-chip moisture content measurement controller.
2. by the described device that is used for solid ferment process maintenance high water activity of claim 1, it is characterized in that: described solid-state fermenter 4 is an airtight withstand voltage 0.3Mpa, horizontal low pressure vessel.
3. by the described device that is used for solid ferment process maintenance high water activity of claim 1, it is characterized in that: the stainless steel reel that described water-absorbing material frame is 0.45m * 0.55m * 0.02m, placement steam puffed stalk or paper pulp are used.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02245657 CN2567225Y (en) | 2002-08-09 | 2002-08-09 | Device for maitaining high water activity in solid state fermentation process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02245657 CN2567225Y (en) | 2002-08-09 | 2002-08-09 | Device for maitaining high water activity in solid state fermentation process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2567225Y true CN2567225Y (en) | 2003-08-20 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 02245657 Expired - Lifetime CN2567225Y (en) | 2002-08-09 | 2002-08-09 | Device for maitaining high water activity in solid state fermentation process |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106381267A (en) * | 2016-08-31 | 2017-02-08 | 西安若翰生物饲料有限公司 | Simple efficient solid fermentation device |
| CN106923050A (en) * | 2017-05-09 | 2017-07-07 | 中科天华生物科技有限公司 | Paper mulberry feed for poultry and preparation method thereof |
| CN107047961A (en) * | 2017-05-09 | 2017-08-18 | 中科天华生物科技有限公司 | Paper mulberry feed and preparation method thereof |
| CN107410670A (en) * | 2017-05-09 | 2017-12-01 | 中科天华生物科技有限公司 | Protein feed containing fermented papermulberry tree and preparation method thereof |
| US10851486B2 (en) | 2015-09-01 | 2020-12-01 | Samsung Electronics Co., Ltd. | Washing machine |
-
2002
- 2002-08-09 CN CN 02245657 patent/CN2567225Y/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10851486B2 (en) | 2015-09-01 | 2020-12-01 | Samsung Electronics Co., Ltd. | Washing machine |
| CN106381267A (en) * | 2016-08-31 | 2017-02-08 | 西安若翰生物饲料有限公司 | Simple efficient solid fermentation device |
| CN106923050A (en) * | 2017-05-09 | 2017-07-07 | 中科天华生物科技有限公司 | Paper mulberry feed for poultry and preparation method thereof |
| CN107047961A (en) * | 2017-05-09 | 2017-08-18 | 中科天华生物科技有限公司 | Paper mulberry feed and preparation method thereof |
| CN107410670A (en) * | 2017-05-09 | 2017-12-01 | 中科天华生物科技有限公司 | Protein feed containing fermented papermulberry tree and preparation method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Effective date of abandoning: 20050921 |
|
| AV01 | Patent right actively abandoned |
Effective date of abandoning: 20050921 |
|
| C25 | Abandonment of patent right or utility model to avoid double patenting |