CN100537775C - A kind of method of coercing strategy raising hyaluronic acid volume of production of fermentation production with intermittent high pH - Google Patents
A kind of method of coercing strategy raising hyaluronic acid volume of production of fermentation production with intermittent high pH Download PDFInfo
- Publication number
- CN100537775C CN100537775C CNB2006100981953A CN200610098195A CN100537775C CN 100537775 C CN100537775 C CN 100537775C CN B2006100981953 A CNB2006100981953 A CN B2006100981953A CN 200610098195 A CN200610098195 A CN 200610098195A CN 100537775 C CN100537775 C CN 100537775C
- Authority
- CN
- China
- Prior art keywords
- production
- hour
- kept
- fermentation
- hyaluronic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
A kind ofly coerce the method that strategy improves hyaluronic acid volume of production of fermentation production, belong to the applied technical field of pH control strategy in optimizing fermentation with intermittent high pH.The present invention's employing is controlled at 7.0,2～16 hours pH at preceding 2 hours pH of fermenting process and coerces: earlier pH is brought up to 8.0～8.5, kept 1 hour, reduce pH to 7.0 again, kept 1 hour, improve pH to 8.0～8.5 again, kept 1 hour, and changed pH control repeatedly, up to fermentation ends.The high pH that has a rest during this time coerces down, and hyaluronic output is brought up to 6.7g/L by the 5.0g/L of contrast (pH is constant 7.0 in control) example, and molecular weight drops to 900,000 by 1,300,000.Utilize the middle low-molecular-weight hyaluronic acid of this strategy preparation to can be used for field of health care food.Because hyaluronic acid viscosity reduces, the efficient when having improved downstream processing.This strategy has certain directive significance to the production of other leavened prod.
Description
Technical field
A kind ofly coerce the method that strategy improves hyaluronic acid volume of production of fermentation production, relate to the applied technical field of pH control strategy in optimizing fermentation with intermittent high pH.
Background technology
Hyaluronic acid is a kind of high-molecular weight mucopolysaccharide, and its two kinds of precursor substances are glucuronic acid and N-acetylglucosamine.Hyaluronic acid can extract from animal tissues such as cockscomb, also can pass through Production by Microorganism Fermentation.Because the fermentation method cost is low, product purity height, microbe fermentation method just progressively replace the tissue extraction method.Hyaluronic research mainly concentrates on Optimizing Conditions of Fermentation to Production by Microorganism Fermentation at present, as Optimum of culture medium, and the optimization of DESIGN OF REACTOR optimization and fermentation pattern.Utilize intermittent high pH to coerce strategy and make the hyaluronic output of raising fermentative production, do not see bibliographical information as yet.
Summary of the invention
The purpose of this invention is to provide and a kind ofly coerce the method that strategy improves hyaluronic acid volume of production of fermentation production with intermittent high pH, utilize the present invention can produce in low-molecular-weight hyaluronic acid, and improve hyaluronic output.
Technical scheme of the present invention: the pH that controls fermented liquid stage by stage improves hyaluronic output, employing is controlled at 7.0 at preceding 2 hours pH of fermenting process, pH coerced in 3～16 hours: earlier pH is brought up to 8.0～8.5, kept 1 hour, reduce pH to 7.0 again, kept 1 hour, improve pH to 8.0～8.5 again, kept 1 hour, and promptly changed the pH control of fermented liquid repeatedly, the 3rd, 5,7,9,11,13, pH was controlled to 8.0～8.5 in 15 hours, kept 1 hour, the 4th, 6,8,10,12,14, pH dropped to 7.0 again in 16 hours, kept 1 hour, up to fermentation ends.
Beneficial effect of the present invention: utilize the present invention can produce in low-molecular-weight hyaluronic acid, and improve hyaluronic output.Hyaluronic molecular weight drops to 900,000 by 1,300,000, and output is brought up to 6.7g/L by 5.0g/L.In low-molecular-weight hyaluronic acid can be used for field of health care food because its viscosity reduces the efficient when having improved downstream processing.High pH of the present invention coerces tactful production to other leavened prod and has certain directive significance.
Embodiment
Comparative examples: the used bacterial classification of the present invention is that (Wen Qi, Liu Dengru, Chen Jian, stifled state become " add tensio-active agent and promote streptococcus zooepidemicus high yield hyaluronic acid " to streptococcus zooepidemicus (Streptococcus Zooepidemicus) H-24, " chemical industry progress " 2006,25 (9), 1089～1094), changeing the inclined-plane earlier cultivated 12 hours in 37 ℃ of incubators, carried out shake flask fermentation then 14 hours, 37 ℃ of temperature, rotating speed 200rpm, in the 7L fermentor tank, fermented 16 hours at last, fermentation condition is: pH7.0, mixing speed 200rpm, Ventilation Rate 1.0vvm.Fermention medium is (g/L): sucrose: 70; Yeast powder: 100; MgSO
47H
2O:2.0; Na
2HPO
4: 5.0; K
2SO
4: 7.0.The hyaluronic molecular weight of product is 1,300,000, and output is 5.0g/L.
Embodiment 1: ferment tank adopts high pH to coerce strategy, the same comparative examples of all the other fermentation conditions.Preceding 2 hours pH of fermenting process are controlled at 7.0,3～16h pH and coerce: earlier pH brought up to 8.0, kept 1 hour, drop to 7.0 again, kept 1 hour, bring up to 8.0 again, kept 1 hour ..., up to fermentation ends (16h).The result: hyaluronic output is 6.0g/L, and molecular weight is 1,000,000.
Embodiment 2: ferment tank adopts high pH to coerce strategy, the same comparative examples of all the other fermentation conditions.Preceding 2 hours pH of fermenting process are controlled at 7.0,3～16h pH and coerce: earlier pH brought up to 8.5, kept 1 hour, drop to 7.0 again, kept 1 hour, bring up to 8.5 again, kept 1 hour ..., up to fermentation ends (16h).The result: hyaluronic output is 6.7g/L, and molecular weight is 900,000.
Claims (1)
1, a kind of method of coercing strategy raising hyaluronic acid volume of production of fermentation production with intermittent high pH, the used bacterial classification that it is characterized in that fermenting is streptococcus zooepidemicus Streptococcus Zooepidemicus H-24, the pH that controls fermented liquid stage by stage improves hyaluronic output, employing is controlled at 7.0 at preceding 2 hours pH of fermenting process, pH coerced in 3～16 hours: earlier pH is brought up to 8.0～8.5, kept 1 hour, reduce pH to 7.0 again, kept 1 hour, improve pH to 8.0～8.5 again, kept 1 hour, and promptly changed the pH control of fermented liquid repeatedly, the 3rd, 5,7,9,11,13, pH was controlled to 8.0～8.5 in 15 hours, kept 1 hour, the 4th, 6,8,10,12,14, pH dropped to 7.0 again in 16 hours, kept 1 hour, up to fermentation ends.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100981953A CN100537775C (en) | 2006-12-08 | 2006-12-08 | A kind of method of coercing strategy raising hyaluronic acid volume of production of fermentation production with intermittent high pH |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100981953A CN100537775C (en) | 2006-12-08 | 2006-12-08 | A kind of method of coercing strategy raising hyaluronic acid volume of production of fermentation production with intermittent high pH |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1978658A CN1978658A (en) | 2007-06-13 |
| CN100537775C true CN100537775C (en) | 2009-09-09 |
Family
ID=38129964
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006100981953A Expired - Fee Related CN100537775C (en) | 2006-12-08 | 2006-12-08 | A kind of method of coercing strategy raising hyaluronic acid volume of production of fermentation production with intermittent high pH |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100537775C (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101294180B (en) * | 2008-05-26 | 2012-04-18 | 江南大学 | Method for preparing small-molecular weight hyaluronic acid by adding hydrogen phosphide and ascorbic acid in course of fermentation |
| CN101294181B (en) * | 2008-05-26 | 2010-12-15 | 江南大学 | Method for improving hyaluronic acid volume of production of fermentation production by adding oxygen carrier dodecane |
| CN103320484A (en) * | 2013-06-28 | 2013-09-25 | 四川柯森油田化学有限公司 | Method for improving the fermentation yield of hyaluronic acid (HA) |
-
2006
- 2006-12-08 CN CNB2006100981953A patent/CN100537775C/en not_active Expired - Fee Related
Non-Patent Citations (4)
| Title |
|---|
| 原生质体诱变选育透明质酸菌株及发酵条件的优化. 罗强，孙启玲.四川大学学报(自然科学版)，第40卷第5期. 2003 |
| 原生质体诱变选育透明质酸菌株及发酵条件的优化. 罗强，孙启玲.四川大学学报(自然科学版)，第40卷第5期. 2003 * |
| 透明质酸的发酵调控研究. 田毅红.三峡大学学报(自然科学版)，第26卷第6期. 2004 |
| 透明质酸的发酵调控研究. 田毅红.三峡大学学报(自然科学版)，第26卷第6期. 2004 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1978658A (en) | 2007-06-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102586216B (en) | Method for producing sodium alginate lyase by utilizing vibrio vulnificus | |
| CN101215592B (en) | Fermentation method for producing pullulan polysaccharide | |
| CN103103130B (en) | Production method for lipid through mixed culture of microbes | |
| CN101560537B (en) | Fermentation method for producing high viscocity xanthan gum by xanthomonas campestris | |
| CN103320484A (en) | Method for improving the fermentation yield of hyaluronic acid (HA) | |
| CN102337312A (en) | Method for increasing yield of chondroitin sulfate produced by fermentation method | |
| CN100537775C (en) | A kind of method of coercing strategy raising hyaluronic acid volume of production of fermentation production with intermittent high pH | |
| CN103103126B (en) | Production method for lipid through coupled culture of microbes | |
| CN103952447A (en) | Method for producing succinic acid by fermentation under anaerobic condition | |
| CN101638674B (en) | Method for manufacturing citric acid by utilizing cane sugar hydrolysate fermentation method | |
| CN104845958A (en) | Large-scale production method of chondroitinase | |
| CN104357507B (en) | A kind of high concentration L sorbose fermentation manufacturing techniques | |
| CN105779532A (en) | Method for producing chondroitin sulfate | |
| CN101294181B (en) | Method for improving hyaluronic acid volume of production of fermentation production by adding oxygen carrier dodecane | |
| CN105624213B (en) | A method of 2,3- butanediol is produced using microalgae for raw material | |
| CN105624212B (en) | A method of 2,3- butanediol is produced by raw material of microalgae | |
| CN103352061A (en) | Method for improving output of chondroitin sulfate analoge prepared by fermentation method | |
| CN101294182A (en) | Method for improving hyaluronic acid volume of production of fermentation production with two-stage oxygen dissolving control technology | |
| CN102041285B (en) | Method for fermenting and producing Tremella polysaccharides by adopting constant pH feeding strategy | |
| CN101532040B (en) | Method for producing hyaluronic acid by continuous addition of glucose and fermentation according to acid output | |
| CN101294178A (en) | Method for improving hyaluronic acid volume of production of fermentation production with two-stage cultivation method | |
| CN101698836B (en) | Method for supplementing material in the fermentation process of transglutaminase | |
| CN101386817A (en) | Method for producing yeast single cell protein by blue algae fermentation | |
| KR20090090855A (en) | Large-scale production of b-glucan through semi-continuous fermentation performed with sparassis crispa mycelia | |
| CN103834708A (en) | Efficient production method of biological polysaccharide gellan gum |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090909 Termination date: 20151208 |
|
| EXPY | Termination of patent right or utility model |