CN1219872C - Method and device for keeping high water activity in solid ferment process - Google Patents
Method and device for keeping high water activity in solid ferment process Download PDFInfo
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- CN1219872C CN1219872C CN 02126040 CN02126040A CN1219872C CN 1219872 C CN1219872 C CN 1219872C CN 02126040 CN02126040 CN 02126040 CN 02126040 A CN02126040 A CN 02126040A CN 1219872 C CN1219872 C CN 1219872C
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Abstract
The present invention relates to a method and a device for keeping high water activity in the process of solid-state fermentation. The device comprises a solid-state fermentor, wherein the wall of the fermentor is provided with an air inlet pipe used for installing an air inlet electromagnetic valve and an exhaust outlet, and a water absorption material frame is arranged in the solid-state fermentor; an ultrasonic atomizer is arranged in the middle between both ends of the solid-state fermentor, and a spraying head is arranged on the bottom of the solid-state fermentor; and a power supply of the ultrasonic atomizer is electrically connected with the ultrasonic atomizer by a monolithic computer hygrostat. The switch of the ultrasonic atomizer is realized by on-line control of the monolithic computer hygrostat according to humidity requirements of different microorganisms to different fermentation stages in the process of fermentation, the water activity or the humidity can be flexibly controlled in the process of the solid-state fermentor in the solid-state fermentation, and the goals of aseptic operation and no foreign bacteria which are too hard to realize in a traditional watering or moistening method are realized. The device has the advantages of simple structure and convenient operation.
Description
Technical field
The present invention relates to a kind of method and device thereof of fermentation, particularly a kind of fermentation keeps the method and the device thereof of high water activity in solid ferment process.
Background technology
Modern fermentation industry is the main part of producing with industrialized mode in the contemporary biotechnology industry, one of important symbol that its fermentation technique level is a national biotechnology industry developmental level.As everyone knows, microbial fermentation processes has two classes: liquid submerged fermentation and solid state fermentation.The large-scale industrial production of penicillin in 1945 has been started liquid submerged fermentation technology and modern fermentation industry.But the productivity ratio liquid submerged fermentation of many modern biological products solid state fermentations is much higher, on the other hand, the bulk fermentation waste water that liquid submerged fermentation produces, ventilation and churned mechanically high power consumption, become the obstacle that liquid submerged fermentation further develops again, force it to develop to high density, high viscosity direction.Yet high density, the full-bodied limit are exactly solid state fermentation.Solid-state fermentation technology is that the human use microorganism benefits one of the most ancient technology of oneself.Before several thousand, China just utilizes this technology wine brewing and makes various seasonings.So far it is still the main mode of producing traditional food and beverage.Solid state fermentation can the production zymin, single cell protein, organic acid, alcohol and biotic pesticide etc.Solid state fermentation is also to have obtained using widely at the processing of biological transformation of energy, solid-state castoff, the aspects such as production of meta-bolites at present.Characteristics such as fermentation costs is low because solid state fermentation has, and cost of material is cheap, does not have " three wastes " discharging, and environmental pollution is little, and technological process is simple at present, progressively obtain the attention of countries in the world.
But, because the characteristics of solid state fermentation maximum do not have free-water exactly, thereby the variation of substrate moisture content, will inevitably produce material impact to microbial growth and metabolic capacity.Water activity (a
w) the expression microorganism is to the accessibility of water, the water activity of matrix be influence microorganism can be on substrate the key factor of normal growth, it is relevant with the water content of substrate.And move in the thermal process at solid state fermentation, the moisture evaporation cooling accounts for and moves 80% of heat, along with the increase that moves hot speed, will inevitably cause reducing of soil humidity, changes a
wSize.Therefore, how keeping higher water activity in the solid ferment process is the bottle footpath problem of solid state fermentation widespread use.Document WO 9418306A1 introduces among the BO1F/30 to some extent.At present, directly spray water or the direct method of humidifying air, exist and be difficult to accomplish aseptic technique, easily dye assorted bacterium, to shortcomings such as the solid medium humidification are inhomogeneous at solid ferment process.
Summary of the invention
One of purpose of the present invention is to overcome traditional humidification method for supplementing water and the device difficult shortcoming that keeps than high water activity and easily dye assorted bacterium in solid ferment process; In addition to shortcoming such as the solid medium humidification is inhomogeneous.Two of purpose of the present invention is integrated application ultrasonic atomizatio humidification, fermentor tank pressure pulsation spraying humidification and water-absorbing material evaporation humidification process, finishes humidification in solid-state fermenter; Thereby provide a kind of even to the solid medium humidification, and according to the requirement difference of microorganism to water activity, but the method and the device thereof of the maintenance high water activity in solid ferment process of flexible humidification method.
The object of the present invention is achieved like this:
A kind of device that keeps high water activity in solid ferment process provided by the invention comprises: a solid-state fermenter has the inlet pipe and the venting port that are installed into the pneumoelectric magnet valve, humidity sensor and micro-chip humidity controller on its tank skin; It is characterized in that also comprising: water-absorbing material frame, fog-spray nozzle, ultrasonic atomizer; Wherein evenly be divided into trilaminar water-absorbing material frame and be installed in the solid-state fermenter, ultrasonic atomizer is at the middle part at solid-state fermenter two ends; This ultrasonic atomizer has a plurality of fog-spray nozzles, and is installed at the end of solid-state fermenter, and the ultrasonic atomizer power supply is electrically connected with ultrasonic atomizer by micro-chip moisture content measurement controller.
Described solid-state fermenter is an airtight withstand voltage 0.3Mpa, horizontal low pressure vessel.
The stainless steel reel that described water-absorbing material frame is 0.45m * 0.55m * 0.02m, placement steam puffed stalk or paper pulp are used.
Described ultrasonic atomizer has the fog-spray nozzle more than 3 or 3.
A kind of method that keeps high water activity in solid ferment process provided by the invention is to carry out in isolated plant of the present invention, comprises the steps:
The first, at first in airtight solid-state fermenter, packing in addition is placed on the interior water-absorbing material of water-absorbing material frame, and its Intake Quantity is the 10%---50% (w/w) of solid state fermentation culture material dry weight, under 0.1Mpa steam, and sterilizing air 30 minutes;
Described water-absorbing material is meant paper pulp, steam puffed stalk, sponge, vermiculite etc., in solid ferment process, utilize the continuous evaporation of the water that sorbing material inhales and humidification, thereby maintenance water balance, the moisture that water-absorbing material evaporated is replenished by the water of condensation in the solid-state fermenter, needn't add the moisture of external source; This method is simple and reliable, is common to the microorganism solid fermentation process;
The second, in whole fermentation process, come regularly to start the pitch time that the ultrasonic atomizatio humidifier of installing in airtight solid-state fermenter carries out humidification according to the physical property of fermented product; In solid ferment process, change regularly humidification according to humidity in the jar, this method is common to airtight solid ferment process.
Also comprise and in solid ferment process, cooperate another pressure pulsation technology to carry out humidification again, utilize pressure pulsation device, sterile air inlet pipe from the wall of solid-state fermenter periodically feeds sterile air in airtight solid-state fermenter, improve tank pressure to 0.1---0.2Mpa; Utilize this punching course, make the sterilized water spraying humidification in the solid-state fermenter; This method is applicable to the air pressure oscillation fermenter process.
The invention has the advantages that:
The present invention keeps the method for high water activity in solid ferment process, in isolated plant of the present invention, carry out, be based on the characteristics of solid ferment process, the evaporation of integrated application water-absorbing material humidification, ultrasonic atomizatio humidifying technology and fermentor tank pressure pulsation spraying humidification, enlarge evaporation area and humidification, keep water balance.Method of the present invention can be used the micro-chip humidity controller and control water activity or humidity in the solid-state fermenter process flexibly, thereby reach even to the solid medium humidification according to the different microorganisms or the solid state fermentation in different fermentations stage.The humidification of fermenting process is finished in solid-state fermenter in addition, has avoided tradition to add water or air-humidification method is difficult to accomplish aseptic technique, easily dyes the shortcoming of assorted bacterium.This method is simple to operate, flexibly, and safety, effectively.
That device of the present invention has is simple in structure, be easy to promote large-scale industrialization production.
Description of drawings
The apparatus structure synoptic diagram of maintenance high water activity in solid ferment process of the present invention;
The drawing explanation:
1 water-absorbing material frame; 2 fog-spray nozzles; 3 ultrasonic atomizers;
4 solid-state fermenters, 5 humidity sensors, 6 micro-chip humidity controllers;
7 air inlet magnetic valves; 8 sterile air inlet pipe; 9 solid fermentation materials;
Embodiment
Embodiment 1
Make the device of a maintenance high water activity in solid ferment process of the present invention by Fig. 1
Comprise: airtight, withstand voltage 0.3Mpa, a horizontal low pressure vessel is as solid-state fermenter 4, install one in this solid-state fermenter 4 and evenly be divided into trilaminar water-absorbing material frame 1, this water-absorbing material frame 1 is 0.45m * 0.55m * 0.02m stainless steel reel, and placement steam puffed stalk or paper pulp are used; Installation one has the inlet pipe 10 of 3 air inlet magnetic valves 7 on its tank skin, and a venting port 11 is installed on its tank skin, and humidity sensor 5, micro-chip humidity controller 6, fog-spray nozzle 2 and ultrasonic atomizer 3 all adopt commercially available; Ultrasonic atomizer 3 at solid-state fermenter 4 two ends longitudinally the middle part; This ultrasonic atomizer 3 has 8 fog-spray nozzles 2, and is installed in respectively uniformly at the end of solid-state fermenter 4, and the ultrasonic atomizer power supply is electrically connected with commercially available ultrasonic atomizer 3 by a common micro-chip moisture content measurement controller 6.
Embodiment 2
In the device that embodiment 1 makes, utilize the method for maintenance high water activity in solid ferment process of the present invention to carry out solid state fermentation, present embodiment is to use steam puffed stalk or paper pulp as the adsorption moisture material, may further comprise the steps:
(1) put in the water-absorbing material frame 1 in solid-state fermenter 4 be the steam puffed stalk of 0.1 times of solid culture material or paper pulp as adsorption moisture material 9, under 0.1Mpa steam, sterilizing air 30 minutes;
(2) after the cooling, on the fermentation frame, place postvaccinal solid culture material according to a conventional method and carry out cultivation and fermentation;
(3) in solid ferment process, kept the 0.1Mpa high pressure 5 minutes; 0.05Mpa low pressure 20 minutes, like this, high pressure-low pressure cycle circulation.In by low pressure rising pressure process, sterile air makes a jar interior condensate water atomizing humidification by fog-spray nozzle;
(4) during the fermentation, according to different microorganisms or different fermentations stage to humidity requirement, the unlatching by micro-chip moisture content measurement controller on-line Control ultrasonic atomizer makes whole solid ferment process remain on the relative humidity of 90---95%.
Embodiment 3
On the device of embodiment 1, also be included in solid-state fermenter 4 common pressure pulsation device is installed, during the fermentation, solid-state fermenter is carried out timing pressure pulsation, in the process of boosting, sterile air from the fog-spray nozzle ejection, makes a jar interior condensate water atomizing humidification by sterile air inlet pipe 8.Difference is in addition: put in the water-absorbing material frame 1 in solid-state fermenter 4 be the steam puffed stalk of 0.5 times of solid culture material or paper pulp as adsorption moisture material 9, under 0.1Mpa steam, sterilizing air 30 minutes;
Embodiment 4
In the device that embodiment 1 makes, the method for utilizing maintenance high water activity in solid ferment process of the present invention is to use sponge to carry out solid state fermentation as the adsorption moisture material.
(1) putting in the water-absorbing material frame 1 in solid-state fermenter 4 is the sponge of 0.05 times of solid culture material, under 0.1Mpa steam, and sterilizing air 30 minutes;
(2) after the cooling, on the fermentation frame, place postvaccinal solid culture material according to a conventional method and carry out cultivation and fermentation;
(3) in solid ferment process, kept the 0.15Mpa high pressure 5 minutes, 0.05Mpa low pressure 20 minutes, like this, high pressure-low pressure cycle circulation.In by low pressure rising pressure process, sterile air makes a jar interior condensate water atomizing humidification by fog-spray nozzle;
(4) during the fermentation, according to different microorganisms or different fermentations stage to humidity requirement, the unlatching by micro-chip moisture content measurement controller on-line Control ultrasonic atomizer makes whole solid ferment process remain on the relative humidity of 90---95%.
Embodiment 5
In the device that embodiment 1 makes, the method for utilizing maintenance high water activity in solid ferment process of the present invention is to use vermiculite to carry out solid state fermentation as the adsorption moisture material.
(1) putting in the water-absorbing material frame 1 in solid-state fermenter 4 is the vermiculite of 0.2 times of solid culture material, under 0.1Mpa steam, and sterilizing air 30 minutes;
(2) after the cooling, on the fermentation frame, place postvaccinal solid culture material according to a conventional method and carry out cultivation and fermentation;
(3) in solid ferment process, kept the 0.2Mpa high pressure 5 minutes, 0.05Mpa low pressure 20 minutes, like this, high pressure-low pressure cycle circulation.In by low pressure rising pressure process, sterile air makes a jar interior condensate water atomizing humidification by fog-spray nozzle;
(4) during the fermentation, according to different microorganisms or different fermentations stage to humidity requirement, the unlatching by micro-chip moisture content measurement controller on-line Control ultrasonic atomizer makes whole solid ferment process remain on the relative humidity of 9---95%.
The present invention is based on the characteristics of solid ferment process, the evaporation of integrated application water-absorbing material humidification, fermentor tank pressure pulsation spraying humidification and ultrasonic atomizatio humidifying technology.Respectively water-absorbing material steam puffed stalk, paper pulp, sponge or vermiculite on a kind of airtight, withstand voltage 0.3Mpa, horizontal solid-state fermenter inner bottom part, middle part and top aspect, under 0.1Mpa steam, the sky 30min that disappears; After the cooling, on the fermentation frame, place postvaccinal cultivation material, control certain temperature cultivation and fermentation; In fermenting process, solid-state fermenter is carried out timing pressure pulsation, in giving birth to the pressure process, sterile air makes a jar interior condensate water atomizing humidification by fog-spray nozzle; During the fermentation, according to different microorganisms or different fermentations stage to humidity requirement, by the unlatching of micro-chip moisture content measurement controller on-line Control ultrasonic atomizer.
Claims (6)
1, a kind of device that keeps high water activity in solid ferment process comprises:
One solid-state fermenter (4) has the inlet pipe (10) and the venting port (11) that are installed into pneumoelectric magnet valve (7), humidity sensor (5) and micro-chip humidity controller (6) on its tank skin; It is characterized in that, also comprise: water-absorbing material frame (1), fog-spray nozzle (2), ultrasonic atomizer (3); Wherein evenly be divided into trilaminar water-absorbing material frame (1) and be installed in the solid-state fermenter (4), ultrasonic atomizer (3) at solid-state fermenter (4) two ends longitudinally the middle part; The fog-spray nozzle (2) of this ultrasonic atomizer (3) is installed at the end of solid-state fermenter (4), and the ultrasonic atomizer power supply is electrically connected with ultrasonic atomizer (3) by micro-chip moisture content measurement controller (6); Described solid-state fermenter (4) is horizontal low pressure vessel.
2, by the device of the described maintenance high water activity in solid ferment process of claim 1, it is characterized in that described solid-state fermenter (4) is the fermentor tank of an airtight withstand voltage 0.3Mpa.
3, by the device of the described maintenance high water activity in solid ferment process of claim 1, it is characterized in that described water-absorbing material frame (1) is the stainless steel reel of 0.45m * 0.55m * 0.02m.
4, by the device of the described maintenance high water activity in solid ferment process of claim 1, it is characterized in that described ultrasonic atomizer (3) has 3 or 3 above fog-spray nozzles (2).
5, a kind of application rights requires the device of 1 described maintenance high water activity in solid ferment process to carry out the method for solid state fermentation, it is characterized in that, comprises the steps:
First, at first in airtight solid-state fermenter, pack in water-absorbing material frame (1) solid state fermentation culture material (9) and water-absorbing material, the Intake Quantity of described water-absorbing material are the 10%-50% (w/w) of solid state fermentation culture material dry weight, under 0.1Mpa steam, sterilizing air 30 minutes;
The second, in whole fermentation process, regularly starting the ultrasonic atomizatio humidifier of installing in airtight solid-state fermenter carries out humidification according to different solid culture materials or different fermentations stage.
6, by the method for the described solid state fermentation of claim 5, it is characterized in that:
In solid ferment process, cooperate pressure pulsation technology to replenish moisture content, in airtight solid-state fermenter, feed sterile air periodically, improve tank pressure to 0.1-0.2Mpa by the sterile air inlet pipe (8) on the solid state fermentation tank skin; Utilize this pressurising process, make the sterilized water spraying humidification in the solid-state fermenter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02126040 CN1219872C (en) | 2002-08-09 | 2002-08-09 | Method and device for keeping high water activity in solid ferment process |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02126040 CN1219872C (en) | 2002-08-09 | 2002-08-09 | Method and device for keeping high water activity in solid ferment process |
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| CN1473922A CN1473922A (en) | 2004-02-11 |
| CN1219872C true CN1219872C (en) | 2005-09-21 |
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| CN 02126040 Expired - Fee Related CN1219872C (en) | 2002-08-09 | 2002-08-09 | Method and device for keeping high water activity in solid ferment process |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102584380A (en) * | 2012-02-13 | 2012-07-18 | 侯澄友 | Humidifying device for fermentation tank for preparing biological organic fertilizer |
| CN103397070A (en) * | 2013-07-30 | 2013-11-20 | 中国检验检疫科学研究院 | Sensing and monitoring control method for escherichia coli water activity in bread |
| CN107279793A (en) * | 2017-07-31 | 2017-10-24 | 贞丰县布依农特产品有限公司 | A kind of sausage and preparation method thereof |
| CN108570463B (en) * | 2018-05-02 | 2021-12-24 | 新乡医学院 | Method for producing acid protease by using microorganisms |
| CN110499248B (en) * | 2019-07-31 | 2022-12-16 | 江苏大学 | Solid state fermentation humidity control system |
| CN115553376B (en) * | 2022-09-30 | 2023-12-01 | 博益德（北京）生物科技有限公司 | Method for determining optimal water content of mixed feed fermentation and application thereof |
| CN115553377B (en) * | 2022-09-30 | 2023-11-24 | 博益德（北京）生物科技有限公司 | Method for determining optimal water content of single feed raw material fermentation and application thereof |
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