CN1899080A - Active protein feed and its preparing method - Google Patents
Active protein feed and its preparing method Download PDFInfo
- Publication number
- CN1899080A CN1899080A CNA2005100843751A CN200510084375A CN1899080A CN 1899080 A CN1899080 A CN 1899080A CN A2005100843751 A CNA2005100843751 A CN A2005100843751A CN 200510084375 A CN200510084375 A CN 200510084375A CN 1899080 A CN1899080 A CN 1899080A
- Authority
- CN
- China
- Prior art keywords
- bacterial classification
- raw material
- koji
- active protein
- feed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention discloses a kind of active protein feed and its preparation process. The active protein feed is prepared with the materials comprising fresh solid fermented distiller's grains 96-98 wt%, urea 0.5-1 wt%, ammonium sulfate 1-3 wt% and koji tray strain 0.1-0.6 wt%, and the preparation process is simple, low in labor strength and low in cost. The said process can eliminate the pollution caused by distiller's grains while producing loose feed with special fragrance, rich protein, vitamins, amino acid, biotin mineral and nutritious elements essential for animal growth.
Description
Technical field
The present invention relates to a kind of active protein feed, relating in particular to a kind of is active protein feed of being prepared from of fermenting raw materials and preparation method thereof with bright solid bran koji vinasse mainly, belongs to field of fodder.
Background technology
Active protein feed is higher than plant protein fodder from the biological value nutritive value, is lower than animal protein feed.It is rich in the necessary nutrients of multiple growth of animal such as amounts of protein, amino acid, biotin, mineral matter, biology enzyme, wherein biology enzyme helps animal digesting and assimilating feed nutrient, improve meat feed ratio, can also effectively alleviate of the pollution of animal kenosis environment.Active protein feed can be used as raw material and is widely applied in the middle of all feeds, replaces the complex enzymes that protein raw material and feed added such as wheat bran, grouts etc., and purposes is wide, market prospects good.
Vinasse itself are rich in micro substances such as a large amount of amino acid, biotin, mineral matter, are that growth of animal is necessary, also are that microorganism metabolism, growth and breeding are needed, and this class material just need not add can satisfy the production of fodder demand fully.
Nutritional labeling in the vinasse is except that from the incomplete and residual raw material remnants of diastatic fermentation, mainly from thalline and metabolism product and thalline autolysate.Nutritional labeling in the vinasse is that institutes such as cereal, corn are irreplaceable, it not only contains a large amount of crude protein, thick starch, crude fibre, crude fat, but also be rich in micro-beneficiating ingredient and biology enzyme and other bioactivators such as riboflavin, thiamine, biotin, choline, mineral matter.Therefore the composition of vinasse is quite complicated and enrich, loses contaminated environment, is again an individual great wasting of resources as cheap use of fertilizer only.
In sum, reducing the harmful substance to animal that is contained in the vinasse, is a kind of protein content height of feedstock production with vinasse, and nutritious, the protein feed of good palatability has important practical significance.
Many employing distills ' grainses are that raw material is produced active protein feed when up to now, being the feedstock production protein feed with vinasse.But the distills ' grains yeast phase is long, the acid content height, and the harmful substance that causes suppressing growth of microorganism, breeding is many, is unfavorable for directly cultivating the micro-organisms active protein feed.Adopt the acid-base neutralization method, though can solve the acidity problem in the distills ' grains, other harmful components still can not be improved, and are unfavorable for microbial growth, breeding equally, influence fermentation and quality of the fodder, and economic benefit is lower.For a long time, distills ' grains is mostly handled by cheapness, has done fertilizer.When utilizing distills ' grains for the feedstock production active protein feed, must add other a large amount of protein raw materials, for example rapeseed dregs, the cottonseed dregs of rice etc. water down, dilute harmful components, can use.Owing to added new material, must carry out cooking disinfection again again, so not only improved production cost, also increased labour intensity greatly simultaneously, be unfavorable for the utilization again of the big production vinasse of liquor-making enterprises scale.
Summary of the invention
The present invention's technical problem at first to be solved is to overcome the deficiencies in the prior art, provides that a kind of production cost is low, preparation technology is simple and direct, protein content is high and is rich in the active protein feed of multiple complex enzyme.
The present invention's technical problem at first to be solved realizes by following technological approaches:
A kind of active protein feed, mainly make by following raw materials by weight percent:
Bright solid bran koji vinasse 96-98%, urea 0.5-1%, ammonium sulfate 1-3%, koji tray bacterial classification 0.1-0.6%.
Be preferably: bright solid bran koji vinasse 98%, urea 0.6%, ammonium sulfate 1%, koji tray bacterial classification 0.4%.
Described koji tray bacterial classification is selected from any one or two kinds in aspergillus niger, aspergillus oryzae or the candida utili koji tray bacterial classification.
In the above-mentioned raw materials, the preparation method of aspergillus niger (Aspergillus niger), aspergillus oryzae (Aspergillus oryzae) or candida utili (c.utils) koji tray bacterial classification is well known to those skilled in the art with the preparation of conventional koji tray bacterial classification; Aspergillus niger, aspergillus oryzae or candida utili bacterial classification can be any one commercially available bacterial classification or its mutation (this bacterial classification can available from Microbe Inst., Chinese Academy of Sciences common micro-organisms culture presevation administrative center or Guangdong Microbes Inst culture presevation administrative center).
Described bright solid bran koji vinasse are meant with potato do, corn, Chinese sorghum etc. are raw material, are saccharifying ferment with wheat bran, yeast, behind parallel compound fermentation, distilled spirit, just gone out rice steamer do not have cool to 37 ℃ hot fresh grain stillage.It comprises the general wheat bran wine technology that " Yantai wine brewing maneuver " forms for main fermentation and adopts many bacterial classifications to make saccharifying ferments on this basis, and with reference to the route that uses some technological measure such as Daqu spirit of China, the spirit stillages of producing such as Fen perfume (or spice), Lu perfume (or spice) and thatch odor type, general yeast phase is 3-30 days.
Another technical problem to be solved by this invention provides a kind of method for preparing above-mentioned active protein feed.
Another technical problem to be solved by this invention realizes by following technological approaches, a kind of system
Be equipped with the method for above-mentioned active protein feed, may further comprise the steps:
1) prepares aspergillus niger, aspergillus oryzae or candida utili koji tray bacterial classification by art methods;
2) take by weighing each raw material by following percentage by weight: bright solid bran koji vinasse 96-98%, urea 0.5-1%, ammonium sulfate 1-3%, koji tray bacterial classification 0.1-0.6%;
3) preparation fermentation raw material: urea and ammonium sulfate is made into joins in the wheat bran vinasse behind the aqueous solution and stir, promptly get fermentation raw material; The water content of control fermentation raw material is between 50-57%;
4) inoculation: the temperature for the treatment of fermentation raw material is reduced to 28-34 ℃, uses aspergillus niger, aspergillus oryzae or candida utili koji tray bacterial classification inoculation, postvaccinal raw material is stirred cultivate according to a conventional method into bent room dress ventilation Quchi; Treat that the raw material nutrient is consumed gradually, mycelial growth slowly can go out the pond to stopping growing;
5) product with fermenting-ripening goes out pond, oven dry, pulverizes promptly to get active protein feed.
Among the above-mentioned preparation method, need in the step 3) urea and ammonium sulfate are made into the aqueous solution, look how much and the suitably increase and decrease of the water content of bright solid bran koji vinasse as for the concentration of the aqueous solution, as long as the water content that satisfies fermentation raw material is controlled between the 50-57%, this is that those skilled in the art is easy to grasp and realize; Fermentation materials is gone into dress pond, bent room culture condition and is in the step 4): thickness of feed layer is 20-30cm, and bent room temperature is 25-30 ℃, 28-30 ℃ of product temperature; Be cultured to 6-10 hour, ventilate at interval, control product temperature is about 32 ℃; Be cultured to 10-20 hour, begin continuous ventilating this moment, control product temperature is below 30-34 ℃; Be cultured to 20-26h, grown a large amount of mycelium or somatic cells, Ventilation Control product temperature guarantees eugonic between 34-38 ℃, suitably improve temperature and help product drying.
The prepared active protein feed quality of the present invention is loosened, and color and luster cream colour or greyish white has special Qu Xiang, protein content 〉=25%, moisture account for about 9%, have reached the requirement of albumen fine fodder, whole albumen improves about 8% than distiller's dried grain raw material, is 2.5 times of maize raw material protein.Its nutritive value has substantially exceeded compost, and flavor is fragrant good to eat, the animal good palatability.Feed of the present invention contains bioactivators such as a large amount of carbohydrase, acid protease, cellulase, helps promoting animal that feed digested and assimilated, alleviates effects such as excreta environmental pollution.
The wheat bran vinasse are short because of yeast phase, and acid content is low, suppress the formation negligible amounts of the harmful substance of growth of microorganism breeding, and the nutritional labeling, biotin, the mineral matter that remain in the vinasse the inside are very abundant, are the splendid raw materials of producing active protein feed.The wheat bran vinasse have steamed wine and have gone out rice steamer and loose after the cold, directly inoculation, cultivate, and need not heavily to steam, and have saved the energy and grain resource, have reduced labour intensity, have also reduced production cost significantly.
The residing pH value of distills ' grains scope adapts to mould, saccharomycetic growth, and the pH value of wheat bran vinasse is substantially within mould, the suitableeest saccharomycetic growth scope, it not only helps mould, saccharomycetic growth and breeding, and help in the feed fermentation production process, suppress the pollution of other assorted bacterium, improve the quality of products.
The contained remaining starch in wheat bran vinasse the insides, total reducing sugar are higher, can provide growth of microorganism to breed the needed carbon source and the energy fully.Mould and saccharomycete can not only utilize starch and glucide, under the situation of oxygen supply abundance, can also utilize the alcohols that remains in vinasse the insides, acids, aldehydes etc. as carbon source, directly feed this class material to the toxic action that animal brought thereby reduced vinasse as feed, improved the inherent quality of feed.
In order to reduce the loss of protein, improve quality of the fodder in the cultivation process, the present invention has also added (the NH that is easily utilized by microorganism in raw material
4)
2SO
4Inorganic nitrogen-sourced with urea etc., microorganism can at first utilize inorganic nitrogen to form powerful growth vigor synthetic protein in the growth and breeding process, and this measure can be indirect plays additional mycoprotein, reduces the effects such as loss of material protein.
The direct fermentation of wheat bran vinasse is produced active protein feed and obviously is better than distills ' grains.Its acid content is low, and can but effectively suppress other living contaminants in the cultivation process, and its residing pH value is in saccharomycete, the suitableeest growth scope of mould, more helps the growth of microorganism breeding, need not artificial adjusting.Its yeast phase is short, and the inhibition growth of microorganism of generation, the material of breeding are less, and production is not constituted influence, and need not add other material and water down, dilute, boiling once more, the raw material cool just directly cultivation afterwards of loosing, energy savings has reduced labour intensity.It and distills ' grains contain a large amount of thick starch, crude fibre, crude protein, crude fat and other micro substance equally, and the thick a little higher than distills ' grains of content of starch, more benefit the microbial growth breeding, improve the protein content of feed.
The wheat bran vinasse not only can directly be produced active protein feed as stated above, can also add cotton dregs, the rape dregs of rice etc. after, produce the high protein activated feed through boiling sterilization again.
Further illustrate beneficial effect of the present invention by the following examples, it should be understood that these embodiment only are used for the purpose of illustration, never limit the scope of the invention.
The specific embodiment
Embodiment 1
One, preparation aspergillus niger koji tray bacterial classification, concrete grammar is as follows:
1, the preparation of test tube strains (aspergillus niger strain: CGMCC 3.0040, available from common micro-organisms DSMZ of Institute of Microorganism, Academia Sinica):
(1) test tube sterilization: test tube cleans up, and water is drained, and puts into oven for drying, tampon beyond the Great Wall, 140 ℃ of hot air sterilization 120min.
(2) inclined-plane Czapek's medium preparation
1. Czapek's medium prescription: NaNO
33g, K
2HPO
43H
2O 1g, MgSO
47H
2O 0.5g, FeSO
47H
2O 0.01g, KCl 0.5g, agar 20g, water 1000ml.
2. preparation: get mentioned component and place in the aluminum pot, heating for dissolving is cold slightly, the packing test tube, and tampon beyond the Great Wall in 0.1MPa sterilization 30min down, takes out and puts into the inclined-plane, places 30 ℃ of constant temperature culture afterwards 3 days, checks no condensate water, and nothing can be used by the bacterium of mixing.
3. inoculation: get 1 in sterilized water test tube (about 5ml), 1 in the former bacterium test tube of aspergillus niger, through the qualified slant tube number of close inspection, sterilize with 70% alcohol wipe, put into sterile board, move down at aseptic condition and connect a ring spore in sterilized water, make spore suspension, shake up and move a ring suspension, the inoculation of line test tube.
4. heat insulating culture: the slant tube of inoculation is placed in the insulating box, cultivated 7 days for 31 ℃, can use after check reaches quality requirement.Or place the interior 4 ℃ of conditions of refrigerator to preserve standby.
5. quality requirement: mycelia is neatly healthy and strong, and spore is plentiful dense.Be dark brown, uniformity does not have assorted bacterium, and the culture medium back side is that deep yellow brown has block wrinkle, no diffusate.
2, the preparation of triangular flask bacterial classification
1. batching bottling: wheat bran 100g, water 110ml, (NH
4)
2SO
40.4g after its mixing material moistening, packing 30g wet feed is wrapped brown paper in the 500ml triangular flask.
2. sterilization: the 0.1MPa 30min that sterilizes is cooled to 28-30 ℃.
3. inoculated and cultured: inoculate in sterile board, shake up, place in 31 ℃ of insulating boxs and cultivate, it is even to shake stand, bottle back about 24h, and the button bottle once continues to cultivate 4-5 days maturations after becoming pie about 10h again.
4. quality requirement: mycelia is neatly healthy and strong, and spore is plentiful dense, and inside and outside unanimity is coffee-like, does not have assorted bacterium.
3, the production of koji tray bacterial classification
1. prepare burden: wheat bran 80%, fresh grain stillage 20%, urea 0.5%, amount of water are 80% of wheat bran, with about industrial sulphuric acid adjust pH 4-5.
2. steam material: spice is even, sieves material moistening 30min, atmospheric cooking 1 hour.
3. cooling inoculation is cooled to 34-35 ℃ of inoculation with the bran material, and inoculum concentration 0.2-0.3% mixes accumulation, 30-32 ℃ of product temperature, insulation 6-8h.
4. adorn koji tray: when the product temperature rises 35 ℃ of left and right sides, material is shakeout on koji tray, about thickness 2cm, build Polypropylence Sheet, heat and moisture preserving is cultivated.Relative humidity is controlled at about 95% (wet-bulb depression 1-2 ℃) in indoor.
5. draw curtain: draw koji tray after cultivating 12h, the product temperature control is at 32-34 ℃.
6. heat insulating culture changes the middle and later periods cultivation over to behind stroke koji tray, requires the product temperature to keep in balance, and cultivates about 50h for 32-35 ℃, and spore is mature on the whole, carries out humidity discharging and is cultured to 60-65h, can go out bent room.
7. dry: dry under 36-37 ℃ of condition.
8. plant Qu Zhiliang: cleaning-less bacteria infection, mycelia are neatly healthy and strong, and spore is plentiful dense, are coffee-like, and quality is loose, inside and outside unanimity, and special Qu Xiang is arranged.
9. production hygienic requirements
A, life, grog strictness separate, in order to avoid bacteria infection.
B, koji device therefor, instrument are used wet sterilization before using, and should in time rinse well after using up, and available 5% bleaching fluid sterilization keeps clean.
After c, Cheng Qu went out the room, cleaning down was answered on koji tray, ground, and sterilizes with bleaching fluid.Bent room planted agent regularly uses sulphur, formaldehyde fumigation sterilization.
Two, 0.6kg urea and 1kg ammonium sulfate are made into the aqueous solution; Get 98kg aquatic foods wheat bran using solid distillers ' grains (so-called bright wheat bran vinasse are meant that the intact wine of firm steaming goes out the diffusing as yet cool vinasse of rice steamer) the diffusing cold of air blast to about 35-36 ℃ stream and add urea and ammonium sulfate solution, the fermentation raw material water content is controlled at (notes: refer to pile up moisture) between the 52-54%, is beneficial to cultivation.When expecting cold,, turn evenly, go into the cultivation of bent room dress ventilation Quchi with above-mentioned prepared aspergillus niger koji tray bacterial classification 0.4kg inoculation to 34 ℃.
Cultivate: thickness of feed layer is 20-30cm, and room temperature 28-30 ℃, going into the pond temperature is 30-32 ℃.
A, cultivate early stage: dress pond 6-10h, intermittent air blast is carried out in the bottom out of product temperature when product temperature rises to 32-34 ℃, control the product temperature below 34 ℃.
B, cultivate mid-term: cultivate 10-20h, the beginning of product temperature is risen gradually, enters the growth animated period, begins continuous ventilating at this moment, controls the product temperature below 36 ℃.
C, late stage of culture: cultivate 20-24h, grown a large amount of mycelium, control product temperature is about 38 ℃, and ventilation in good time, guarantees eugonic, suitably improves the product temperature, helps product drying.
Go out the pond: cultivate that the nutrient in the raw material is consumed gradually after 24 hours, moisture constantly reduces mycelial growth slowly can go out the pond to stopping growing, and goes out to drop into drying pool behind the pond and dry, and pulverizes and promptly gets active protein feed.
The prepared active protein feed quality of present embodiment is loosened, color and luster cream colour or greyish white, special Qu Xiang is arranged, protein content 〉=25%, water accounts 9%, also contain the 300-500ug/g carbohydrase, the acid protease of 300-400 unit/g, the biology enzymes such as cellulase of 1476 units/g help animal to digest and assimilate feed is organic, increase meat feed ratio, alleviate the pollution of animal excrements environment.
Embodiment 2
One, preparation aspergillus oryzae koji tray bacterial classification, concrete grammar is as follows:
1, the preparation of test tube strains (aspergillus oryzae bacterial classification: CGMCC3.0320, available from common micro-organisms DSMZ of Institute of Microorganism, Academia Sinica):
(1) test tube sterilization: test tube cleans up, and water is drained, and puts into oven for drying, tampon beyond the Great Wall, 140 ℃ of hot air sterilization 120min.
(2) inclined-plane Czapek's medium preparation
1. Czapek's medium prescription: NaNO
33g, K
2HPO
43H
2O 1g, MgSO
47H
2O 0.5g, FeSO
47H
2O 0.01g, KCl 0.5g, agar 20g, water 1000ml.
2. preparation: get mentioned component and place in the aluminum pot, heating for dissolving is cold slightly, the packing test tube, and tampon beyond the Great Wall in 0.1MPa sterilization 30min down, takes out and puts into the inclined-plane, places 30 ℃ of constant temperature culture afterwards 3 days, checks no condensate water, and nothing can be used by the bacterium of mixing.
3. inoculation: get 1 in sterilized water test tube (about 5ml), 1 in the former bacterium test tube of aspergillus oryzae, through the qualified slant tube number of close inspection, sterilize with 70% alcohol wipe, put into sterile board, move down at aseptic condition and connect a ring spore in sterilized water, make spore suspension, shake up and move a ring suspension, the inoculation of line test tube.
4. heat insulating culture: the slant tube of inoculation is placed in the insulating box, cultivated 7 days for 31 ℃, can use after check reaches quality requirement.Or place the interior 4 ℃ of conditions of refrigerator to preserve standby.
5. quality requirement: mycelia is neatly healthy and strong, and spore is plentiful dense.Be dark brown, uniformity does not have assorted bacterium, and the culture medium back side is that deep yellow brown has block wrinkle, no diffusate.
2, the preparation of triangular flask bacterial classification
1. batching bottling: wheat bran 100g, water 110ml, (NH
4)
2SO
40.4g after its mixing material moistening, packing 30g wet feed is wrapped brown paper in the 500mI triangular flask.
2. sterilization: the 0.1MPa 30min that sterilizes is cooled to 28-30 ℃.
3. inoculated and cultured: inoculate in sterile board, shake up, place in 31 ℃ of insulating boxs and cultivate, it is even to shake stand, bottle back about 24h, and the button bottle once continues to cultivate 4-5 days maturations after becoming pie about 10h again.
4. quality requirement: mycelia is neatly healthy and strong, and spore is plentiful dense, and inside and outside unanimity is coffee-like, does not have assorted bacterium.
3, the production of koji tray bacterial classification
1. prepare burden: wheat bran 80%, fresh grain stillage 20%, urea 0.5%, amount of water are 80% of wheat bran, with about industrial sulphuric acid adjust pH 4-5.
2. steam material: spice is even, sieves material moistening 30min, atmospheric cooking 1 hour.
3. cooling inoculation is cooled to 34 ℃ of inoculations with the bran material, and inoculum concentration 0.3% is mixed accumulation, 32 ℃ of product temperature, insulation 8h.
4. adorn koji tray: when the product temperature rises 35 ℃ of left and right sides, material is shakeout on koji tray, about thickness 2cm, build Polypropylence Sheet, heat and moisture preserving is cultivated.Relative humidity is controlled at about 95% (wet-bulb depression 1-2 ℃) in indoor.
5. draw curtain: draw koji tray after cultivating 12h, the product temperature control is at 32-34 ℃.
6. heat insulating culture changes the middle and later periods cultivation over to behind stroke koji tray, requires the product temperature to keep in balance, and cultivates about 50h for 32-35 ℃, and spore is mature on the whole, carries out humidity discharging and is cultured to 60-65h, can go out bent room.
7. dry: dry under 36-37 ℃ of condition.
8. plant Qu Zhiliang, cleaning-less bacteria infection, mycelia is neatly healthy and strong, and spore is plentiful dense, is coffee-like, and quality is loose, inside and outside unanimity, and special Qu Xiang is arranged.
9. production hygienic requirements
A, life, grog strictness separate, in order to avoid bacteria infection.
B, koji device therefor, instrument are used wet sterilization before using, and should in time rinse well after using up, and available 5% bleaching liquid sterilization keeps clean.
After c, Cheng Qu went out the room, cleaning down was answered on koji tray, ground, and sterilizes with bleaching fluid.Bent room planted agent regularly uses sulphur, formaldehyde fumigation sterilization.
Two, 0.6kg urea and 1kg ammonium sulfate are made into the aqueous solution; Get the diffusing cold of the bright wheat bran vinasse of 98kg air blast to about 36 ℃ streams and add urea and ammonium sulfate, the fermentation raw material water content is controlled between the 50-52% (annotates: refer to pile up moisture), be beneficial to cultivation.Inoculation when expecting cold to 34 ℃, inoculum concentration: above-mentioned prepared aspergillus oryzae koji tray bacterial classification 0.4kg turns and evenly goes into the cultivation of bent room dress ventilation Quchi.
Cultivate: thickness of feed layer is 20-30cm, and room temperature 28-30 ℃, going into the pond temperature is 30-32 ℃.
A, cultivate early stage: dress pond 6-10h, intermittent air blast is carried out in the bottom out of product temperature when the product temperature rises to 32-34 ℃.
B, cultivate mid-term: cultivate 10-20h, the beginning of product temperature is risen gradually, enters the growth animated period, begins continuous ventilating at this moment, controls the product temperature below 36 ℃.
C, late stage of culture: cultivate 20? about 24h, grown a large amount of mycelium, control product temperature is about 36 ℃, and ventilation in good time, guarantees eugonic, suitably improves the product temperature, helps product drying.
Go out the pond: cultivated about 24 hours, the nutrient in the raw material is consumed moisture gradually constantly to be reduced mycelial growth and slowly can go out the pond to stopping growing, and goes out to drop into drying pool behind the pond and dry, and pulverizes, packs, deposits.
The prepared active protein feed quality of present embodiment is loosened, and the color and luster cream colour has special Qu Xiang, protein content 〉=25%, water accounts 8%, and contain bioactivators such as abundant cellulase, amylase, acid protease, help digesting and assimilating of feed, increase meat feed ratio.
Embodiment 3
One, preparation candida utili koji tray bacterial classification, concrete grammar is as follows:
1, the preparation of candida utili test tube strains, triangular flask bacterial classification and Ka Shi jar bacterial classification is advised distiller's yeast usually and is cultivated and to be published by China Light Industry Press referring to " brewing wine with raw materials technology " book, the Huangping chief editor, Liu Yigang, Yu Minghua associate editor (candida utili bacterial classification: available from Guangdong Microbes Inst common micro-organisms culture presevation administrative center, bacterium numbering: GIM2.148).
(1) cultivation of Candida utilis koji tray yeast solid seed:
1. batching: wheat bran 50%, fresh grain stillage 50%, amount of water is 80% of a wheat bran, turns evenly and sieves, and requires no sandwich no agglomerate.
2. steam material: atmospheric cooking 1 hour goes out rice steamer and looses cool to about 30 ℃.
3. inoculation: the yeast Ka Shi jar bacterial classification of the Glucoamylase Solution of 50,000 units that the account for raw material 0.1%/g that has activated and 5-10% is inserted in the above culture medium, stir, loose nothing caking.
4. cultivate: the koji tray of packing into, thickness of feed layer are 2-3cm, and room temperature is controlled at 25-28 ℃, cultivate the product temperature control at 28-30 ℃.6-8 hour product temperature rises, and beginning suitably turns, and turned and ventilated in 10-12 hour, the vigorous breeding of yeast cells about 15 hours, product temperature rises very fast, at this moment will be aeration-cooling and turn.24-30 hour Yeast Cultivation finishes, and improves product temperature to 35 ℃, promotion moisture evaporation.5. seed is placed the low temperature place of drying in the shade, in 10 days, use up.
Two, preparation aspergillus niger koji tray bacterial classification (with embodiment 1).
Three, 0.9kg urea and 1kg ammonium sulfate are made into the aqueous solution; Get the bright wheat bran using solid distillers ' grains of 97.5kg, the diffusing cold of air blast to about 28-30 ℃ stream adds urea and ammonium sulfate solution, the fermentation raw material water content is controlled between the 55-57% (refer to pile up moisture), is beneficial to cultivation.With above-mentioned prepared aspergillus niger koji tray bacterial classification 0.2kg and candida utili koji tray bacterial classification 0.4kg inoculation, turn evenly the Quchi cultivation and fermentation of going into to ventilate.Fermentation condition: thickness of feed layer is at 25-30cm, and blast guarantees that air communication crosses charge level, and room temperature is controlled at 25-28 ℃, cultivates the product temperature control at 28-30 ℃.Ventilated 1 time in every 3-4 of cultivation initial stage hour, each 5-8 minute, after 12 hours, whenever the product temperature rise is ventilated during to 30-32 ℃ at once, control product temperature is below 34 ℃, and is so high even the product temperature fails to be raised to, to ventilate in 3-4 hour 1 time, in case yeast suffocates,, cultivate and got final product maturation in 40 hours if culture medium carbon source abundance and acidity are lower.If under above-mentioned opposite condition, should cultivate about 30 hours.
After cultivating maturation, go out the pond and change the drying pool oven dry over to, pulverizing is deposited, and yield rate accounts for into 80% of pond raw material dry product, and yeast is about 25-28 hundred million/gram, protein content 〉=25%.
Claims (7)
1. an active protein feed is mainly made by following raw materials by weight percent: bright solid bran koji vinasse 96-98%, urea 0.5-1%, ammonium sulfate 1-3%, koji tray bacterial classification 0.1-0.6%; Wherein said koji tray bacterial classification is selected from any one or two kinds in aspergillus niger, aspergillus oryzae or the candida utili koji tray bacterial classification.
2. according to the active protein feed of claim 1, it is characterized in that the percentage by weight of each raw material is: bright solid bran koji vinasse 98%, urea 0.6%, ammonium sulfate 1%, koji tray bacterial classification 0.4%.
3. according to the active protein feed of claim 1, it is characterized in that described bright solid bran koji vinasse be meant with potato do, corn or Chinese sorghum be raw material, with wheat bran, yeast is saccharifying ferment, has just gone out the hot bright using solid distillers ' grains that the rice steamer temperature is not less than 37 ℃ behind parallel compound fermentation, distilled spirit.
4. method for preparing the active protein feed of claim 1 comprises following method:
1) prepares aspergillus niger, aspergillus oryzae or candida utili koji tray bacterial classification by art methods;
2) take by weighing each raw material by following percentage by weight: bright solid bran koji vinasse 96-98%, urea 0.5-1%, ammonium sulfate 1-3%, koji tray bacterial classification 0.1-0.6%; Wherein said koji tray bacterial classification is selected from any one or two kinds in aspergillus niger, aspergillus oryzae or the candida utili koji tray bacterial classification;
3) preparation fermentation raw material: urea and ammonium sulfate are made into the aqueous solution join in the bright solid bran koji vinasse and stir, fermentation raw material; The water content of control fermentation raw material is between 50-57%;
4) inoculation: the temperature for the treatment of fermentation raw material is reduced to 28-34 ℃, uses the koji tray bacterial classification inoculation, postvaccinal raw material is stirred adorn ventilation Quchi cultivation and fermentation according to a conventional method into bent room;
5) will cultivate ripe tunning and go out pond, oven dry, and pulverize and promptly get active protein feed.
5. according to the method for claim 4, it is characterized in that raw material is gone into dress pond, bent room culture condition in the step 4) and be: thickness of feed layer is 20-30cm, and room temperature 25-30 ℃, going into the pond temperature is 28-32 ℃; Be cultured to 6-10h, ventilate at interval, control product temperature is at 32 ℃; Be cultured to 10-20h, continuous ventilating, control product temperature is below 35 ℃; Be cultured to 20-26h, improve product temperature to 38 ℃.
6. claim 1 or 2 the purposes of active protein feed in animal husbandry.
7. according to the purposes of claim 6, it is characterized in that the purposes in animal feed is produced.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100843751A CN100571531C (en) | 2005-07-19 | 2005-07-19 | A kind of active protein feed and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100843751A CN100571531C (en) | 2005-07-19 | 2005-07-19 | A kind of active protein feed and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1899080A true CN1899080A (en) | 2007-01-24 |
| CN100571531C CN100571531C (en) | 2009-12-23 |
Family
ID=37655216
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100843751A Expired - Fee Related CN100571531C (en) | 2005-07-19 | 2005-07-19 | A kind of active protein feed and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100571531C (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101779722A (en) * | 2010-02-10 | 2010-07-21 | 湖北高生生物饲料有限公司 | Yeast culture and preparation method thereof |
| CN102084930A (en) * | 2009-12-04 | 2011-06-08 | 滨州市正元畜牧发展有限公司 | Preparation method of organic manganese enzyme preparation |
| CN101735916B (en) * | 2008-11-13 | 2011-12-28 | 陈尚志 | Fungal fragrant wine and production method thereof |
| CN103564153A (en) * | 2012-07-24 | 2014-02-12 | 黑龙江省轻工科学研究院 | Method for converting non-protein nitrogen into mycoprotein feed |
| CN105009933A (en) * | 2015-06-10 | 2015-11-04 | 邓娟 | Process of cultivating volvariella volvacea by means of aerated koji |
| CN109619285A (en) * | 2019-01-09 | 2019-04-16 | 安徽金种子酒业股份有限公司 | A kind of animal feeding-stuff containing somatic protein and preparation method thereof standby using bent wine wine lees pickling |
| CN112725196A (en) * | 2021-02-06 | 2021-04-30 | 山东金益嘉生物技术有限公司 | Biological agent capable of replacing antibiotics for feed |
| CN112725197A (en) * | 2021-02-06 | 2021-04-30 | 山东金益嘉生物技术有限公司 | Preparation process of biological agent capable of replacing antibiotics for feed |
-
2005
- 2005-07-19 CN CNB2005100843751A patent/CN100571531C/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101735916B (en) * | 2008-11-13 | 2011-12-28 | 陈尚志 | Fungal fragrant wine and production method thereof |
| CN102084930A (en) * | 2009-12-04 | 2011-06-08 | 滨州市正元畜牧发展有限公司 | Preparation method of organic manganese enzyme preparation |
| CN101779722A (en) * | 2010-02-10 | 2010-07-21 | 湖北高生生物饲料有限公司 | Yeast culture and preparation method thereof |
| CN101779722B (en) * | 2010-02-10 | 2012-12-19 | 湖北高生生物饲料有限公司 | Yeast culture and preparation method thereof |
| CN103564153A (en) * | 2012-07-24 | 2014-02-12 | 黑龙江省轻工科学研究院 | Method for converting non-protein nitrogen into mycoprotein feed |
| CN105009933A (en) * | 2015-06-10 | 2015-11-04 | 邓娟 | Process of cultivating volvariella volvacea by means of aerated koji |
| CN109619285A (en) * | 2019-01-09 | 2019-04-16 | 安徽金种子酒业股份有限公司 | A kind of animal feeding-stuff containing somatic protein and preparation method thereof standby using bent wine wine lees pickling |
| CN112725196A (en) * | 2021-02-06 | 2021-04-30 | 山东金益嘉生物技术有限公司 | Biological agent capable of replacing antibiotics for feed |
| CN112725197A (en) * | 2021-02-06 | 2021-04-30 | 山东金益嘉生物技术有限公司 | Preparation process of biological agent capable of replacing antibiotics for feed |
| CN112725196B (en) * | 2021-02-06 | 2023-02-17 | 山东金益嘉生物技术有限公司 | Production process of biological agent capable of replacing antibiotics for feed |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100571531C (en) | 2009-12-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102160595B (en) | Preparation process of complex microorganism fermented active feed | |
| CN101692867B (en) | Method for producing high protein bean pulp by microbial fermentation | |
| CN100571531C (en) | A kind of active protein feed and preparation method thereof | |
| CN106396807A (en) | Culture medium for improving bioconversion rate of edible fungi, preparation method of culture medium and cultivation method of edible fungi | |
| CN103468594B (en) | Candidautilis strain and application thereof | |
| CN101822170A (en) | Method for producing Anrodia camphorata mycelia based on solid-state surface culture | |
| CN106173193A (en) | A kind of method of solid fermentation bean cake | |
| CN101692869B (en) | Method for producing high protein bean pulp based on two-step microbial fermentation | |
| CN1672568A (en) | Saccharifying and aromatizing yeast and its application in fermented jam product | |
| CN103184174B (en) | Production method of bacillus subtilis biological agent used for sodium humate-containing feed in medium | |
| CN103289937B (en) | Method for producing bacillus laterosporus live bacteria by high density solid fermentation | |
| CN104987156A (en) | Lyophyllum fumosurn culture medium using fermented bran and method for cultivating lyophyllum fumosurn | |
| CN104855680A (en) | Pleurotus nebrodensis fungus chaff fermented feed and processing technology thereof | |
| CN101933439A (en) | Method for improving phellinus igniarius hypha amount of submerged culture by utilizing plant oil | |
| CN109997608A (en) | A kind of implantation methods of edible mushroom | |
| CN104543399B (en) | A kind of preparation method of yeast selenium forage additive | |
| CN107712266A (en) | Secondary fermentation grain slag produces the method and application method of high activity high nutrition feed | |
| CN105779300A (en) | Solid culture medium and cultivation method of cordyceps militaris fruiting bodies | |
| CN101851121A (en) | Compound bacterial agent for efficiently converting pig excrements and preparation method and application thereof | |
| CN106173189A (en) | A kind of method of solid fermentation cottonseed meal | |
| CN1171539C (en) | Nutrients additive for biological feed of livestock and fowls and its preparing process | |
| CN109123076A (en) | A kind of production method of livestock and poultry vitamin B2 auxotype probiotics | |
| CN1962488A (en) | Composite microecological agent for aquaculture and scenery water body and its preparation method | |
| CN107760612A (en) | A kind of aspergillus niger yy07 bacterial strains and its application in solid fermentation produces feeding acid protease | |
| CN104381615A (en) | Method for producing cordyceps sinensis feed additive and product |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20091223 Termination date: 20140719 |
|
| EXPY | Termination of patent right or utility model |