CN112725196B - Production process of biological agent capable of replacing antibiotics for feed - Google Patents

Production process of biological agent capable of replacing antibiotics for feed Download PDF

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CN112725196B
CN112725196B CN202110164774.8A CN202110164774A CN112725196B CN 112725196 B CN112725196 B CN 112725196B CN 202110164774 A CN202110164774 A CN 202110164774A CN 112725196 B CN112725196 B CN 112725196B
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cylinder
stirring
guide
rod
constant volume
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CN112725196A (en
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尹鹏
李卫东
李晓燕
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Shandong Jinyijia Biotechnology Co ltd
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Shandong Jinyijia Biotechnology Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/28—Removal of unwanted matter, e.g. deodorisation or detoxification using microorganisms
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/03—Means for pre-treatment of biological substances by control of the humidity or content of liquids; Drying
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/20—Heating; Cooling
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    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

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  • Apparatuses For Bulk Treatment Of Fruits And Vegetables And Apparatuses For Preparing Feeds (AREA)

Abstract

The invention relates to a production process of a biological agent capable of replacing antibiotics for feeds, belonging to the technical field of biological feed preparation. The material is characterized by comprising the following solid raw materials in percentage by weight: 80% of soybean meal; 10 percent of bran; 10% of corn germ meal; the protein content of the soybean meal is 46%, and the moisture content of the bran and the corn germ meal is lower than 13%. The invention designs a biological microbial agent capable of replacing antibiotics for feeds, takes Aspergillus niger raw bacteria as a main biological microbial agent, and can replace antibiotic components in traditional feeds or reduce the using amount of antibiotics. The biological agent can decompose macromolecular saccharides in the feed into monosaccharide or oligosaccharide, generate various organic acids, vitamins, biological enzymes and unknown growth factors, greatly improve the nutritional value and the digestibility of the fermented feed, and improve the animal productivity.

Description

Production process of biological agent capable of replacing antibiotics for feed
Technical Field
The invention belongs to the technical field of biological feed preparation, and particularly relates to a production process of a biological agent capable of replacing feed antibiotics.
Background
Antibiotics are added into the traditional animal feed to reduce the morbidity of the raised animals and further improve the productivity. However, the hazards of antibiotics are well known to be transmitted through the food chain to humans and the surrounding environment, causing irreversible damage.
In order to solve the problem of harm caused by excessive addition of antibiotics in the feed, the modern agricultural feed mostly adopts a biological microbial inoculum or a biological feed additive to replace or reduce the addition of the antibiotics. The biological feed additive is prepared with normal microbe and its metabolic product or growth promoting matter and through special processing, and has the functions of replenishing, regulating or maintaining the microecological balance in animal's intestinal tract, preventing and treating diseases, promoting health and raising production performance. The microbial feed additive comprises normal microbe members, especially dominant microbe groups, and also comprises a preparation prepared from substances capable of promoting the growth and reproduction of the normal microbe groups, and can generate certain biological effect or ecological effect.
The preparation process of the biological agent in the prior art is complex and has non-uniform standard, and the high-quality batch production through automatic equipment is more difficult to realize. In view of this, the applicant designs the patent, which can replace the antibiotic component in the traditional feed or reduce the antibiotic dosage, improve the feed nutritive value and improve the animal productivity.
Disclosure of Invention
In order to solve the problems, the invention provides a production process of a biological agent capable of replacing antibiotics for feeds, which can replace antibiotic components in traditional feeds or reduce the using amount of antibiotics, improve the nutritive value of the feeds and improve the productivity of animals; the second purpose of the invention is to improve the preparation quality and yield of the biological agent and reduce the labor intensity by designing a set of modern production process and matching with special automatic equipment.
The invention relates to a production process of a biological agent capable of replacing feed antibiotics, which comprises the following solid raw materials in percentage by weight:
80% of soybean meal;
10% of bran;
10% of corn germ meal;
the protein content of the soybean meal is 46%, and the moisture content of the bran and the corn germ meal is lower than 13%.
Preferably, the preparation process comprises the following steps:
a. preparing materials, preparing a sterilization yeast room in advance, and adjusting and checking water, electricity and equipment conditions;
b. uniformly mixing each solid raw material with water by using a water feeding and mixing device, and mixing and moistening the water for 10-20 minutes to prepare a mixed material; the mass mixing ratio of the solid raw materials to the water is 5;
c. steaming; introducing the mixed material into a rotary pressurized material steaming tank for steaming; when steaming, introducing steam into the steaming tank to steam the materials; introducing steam until the indication of a pressure gauge of the steam material tank is 0.5Mpa, closing a steam inlet valve, opening an exhaust valve to exhaust air in the tank until the gauge pressure is 0 Mpa, closing the exhaust valve, and opening the steam inlet valve to introduce steam to steam the materials; when the gauge pressure is 2.0 Mpa, properly closing the steam inlet valve, keeping the pressure stable, and steaming for 30 minutes; closing the steam inlet valve, opening the exhaust valve to exhaust steam until the gauge pressure is 0 Mpa, and preparing to discharge; opening the material steaming tank, rotating to a proper position with the tank opening facing downwards, matching with a crushing or material conveying machine to allow the material to flow out of the material steaming tank, and finally rotating the material steaming tank twice at 45 degrees in a forward and reverse direction to allow the material to be discharged completely; after the discharging is finished, cleaning and keeping clean, restoring the steaming tank to the original position, and turning off the operation power supply;
d. cooling and inoculating; preparing a yeast and loading into a inoculator; after the material steaming is finished, pouring the material out of the material steaming tank, sequentially starting the discharging auger, the air cooling machine and the inoculation machine, adjusting the size of a material port of the inoculation machine to a proper position, uniformly and continuously adding the yeast seeds into the inoculation auger to uniformly inoculate the clinker, and receiving the material by a material transfer vehicle and conveying the material to a yeast pool;
e. cultivating; transferring the inoculated and uniformly stirred yeast material into a yeast room, filling into a yeast pool, loosening and flattening to obtain the yeast material with the thickness of about 25cm; adjusting the temperature and the humidity of a koji room, keeping the temperature of the material at 30 to 32 ℃ and the indoor humidity at 90 to 95%, and carrying out heat preservation and moisture preservation culture; the early stage of aspergillus niger culture is a spore germination and hypha formation stage, aspergillus niger spores absorb water and swell to begin germination and form hypha, the growth is stable, the heat production amount is less, and heat preservation and moisture preservation are needed; after 14 to 36 hours, aspergillus niger enters a rapid growth period, the koji material starts to cake, proper ventilation is carried out during the period, the temperature and the humidity are frequently checked and adjusted, the temperature of the material does not exceed 38 ℃, the surface of the koji material does not dehydrate and dry, the whitening of the koji material can be seen after about 24 hours, the surface of the fungus material starts to spore after 36 hours, the fungus material enters a spore generation period, the color of the fungus material gradually changes from light white to black brown, proper cooling and moisturizing are carried out during the period, the temperature of the material is controlled within 32 to 35 ℃, the humidity is controlled within 80 to 90%, and the spore of the fungus material is dense and uniform; after 48 to 60 hours, the culture medium enters the mature period of spores, the spores basically finish forming, the spores continue to grow, the humidity in the culture medium chamber can be properly discharged, the moisture of the culture medium is gradually reduced, the spores are promoted to mature, the culture medium is dense in spore inoculation after 60 to 72 hours, the color is dark brown, the growth is uniform and thorough, the aspergillus niger is cultured to mature, and low-temperature ventilation drying can be carried out;
f. drying the yeast material; when the yeast material is cultured to be close to maturity, ventilating, dehumidifying and drying, or moving the yeast box into a drying chamber for ventilating and drying at the low temperature of 30-40 ℃, paying attention to the fact that the air temperature does not exceed 40 ℃, controlling the temperature in the drying process to be low and high, gradually raising the temperature, preventing spores from being inactivated due to insufficient heat resistance or rapid dehydration, lasting for 12-20 hours, and drying until the moisture is below 12%, wherein the yeast material is aspergillus niger raw bacteria;
g. crushing; feeding Aspergillus niger raw bacteria into a bin through air, crushing to obtain a fineness of 20 meshes, passing the fineness by 70%, crushing, weighing and packaging.
Preferably, the water feeding and mixing device comprises a water feeding mixer and a stirring and moistening device;
the water feeding mixer comprises a first frame, an outer cylinder and an inner cylinder are fixed on the first frame, the inner cylinder is sleeved in the outer cylinder, a material feeding hole is formed in the upper end cover of the outer cylinder, and a material discharging hole is formed in the lower end cover of the outer cylinder; two ends of the inner cylinder are open;
an inner cylinder driving device is fixed on the outer cylinder and can drive the inner cylinder to rotate in the outer cylinder;
the water tank is fixed on the first rack and connected with the spraying and soaking mechanism through a water pump and a flow guide pipe, the spraying and soaking mechanism is arranged in the inner barrel body in a plug-in manner from bottom to top, and can spray and soak materials in the inner barrel body.
Preferably, the inner cylinder driving device comprises an inner cylinder driving motor, a transmission gear set and an inner gear sleeve; the inner cylinder driving motor is fixed on the outer wall of the outer cylinder, and an inner gear sleeve is sleeved and fixed on the outer wall of the inner cylinder; the transmission gear set comprises a driving gear and a middle transmission gear, the driving gear is installed on a motor shaft of the driving motor of the inner cylinder, a transmission hole is formed in the outer wall of the outer cylinder, the middle transmission gear is installed in the transmission hole, and the driving gear is meshed with an inner gear sleeve on the inner cylinder through the middle transmission gear;
an annular guide sliding rail is arranged on the inner wall of the outer cylinder body or the inner wall of the upper end cover or the inner wall of the lower end cover, more than three guide pulleys are annularly arranged on the outer wall of the inner cylinder body corresponding to the position of the annular guide sliding rail, and each guide pulley is arranged in the guide sliding rail;
when the inner cylinder driving motor is started, the inner cylinder can be driven to rotate in the outer cylinder through the meshing of the transmission gear set.
Preferably, the spraying and soaking mechanism comprises a spraying support pipe, and the spraying support pipe is inserted into the inner cylinder from bottom to top; a lifting driving rack is longitudinally fixed on the outer wall of the extending end of the spraying supporting tube, a supporting tube driving motor is fixed at the bottom of the lower end face of the outer cylinder body, a rack driving gear is fixed on a motor shaft of the supporting tube driving motor, and the rack driving gear is meshed with the lifting driving rack;
an upper spraying pipe group and a lower spraying pipe group are fixed at the insertion end of the spraying supporting pipe;
the upper spraying pipe group and the lower spraying pipe group respectively comprise at least two spraying arms, and each spraying arm comprises a front arm rod and a rear arm rod; a front arm rod jack is formed in the pipe wall of the spraying support pipe, a spherical cavity is arranged inside the front arm rod jack, a spherical joint is arranged at the insertion end of the front arm rod, and a hollow pipe cavity is arranged in the front arm rod; the front arm rod is embedded and fixed in the spherical cavity of the front arm rod jack through a spherical joint; the guide pipes penetrate upwards along the spraying support pipe and are connected with the front ends of the guide branch pipes through multi-way joints, the rear ends of the guide branch pipes are communicated with the inner cavity of the spherical joint, and the inner cavity of the spherical joint is communicated with the hollow pipe cavity of the front arm rod; more than one rotary switch valve plate is arranged in the hollow tube cavity of the front arm rod through a spring articulated shaft; the inserting end of the rear arm rod is inserted into the hollow pipe cavity of the front arm rod from the extending end of the front arm rod; the outer extending end of the rear arm rod is hinged to the lower end of the spray arm fixing support rod, and the upper end of the spray arm fixing support rod is fixedly arranged on the upper end cover or the lower end cover of the outer cylinder body; a hollow tube cavity is arranged in the rear arm rod, a plurality of spraying holes are formed in the outer peripheral wall of the rear arm rod, and the spraying holes are communicated with the hollow tube cavity of the rear arm rod;
when a supporting tube driving motor is started to rotate in a forward and reverse fixed angle mode, the lifting driving rack and the spraying supporting tube can be driven to move up and down in a reciprocating mode through meshing of a rack driving gear, then each front arm rod is made to move up and down, the insertion end of the rear arm rod is made to move in a reciprocating mode along the hollow tube cavity of the front arm rod, when the rear arm rod is inserted into the innermost side of the front arm rod, the rotary switch valve plate can be pushed open, and infiltrating water enters the hollow tube cavity of the rear arm rod from the flow guide tube, the flow guide branch tube, the inner cavity of the spherical joint and the hollow tube cavity of the front arm rod and is sprayed out from the spraying holes to infiltrate materials; when the rear arm rod moves outwards, the hollow tube cavity of the front arm rod can be automatically closed by rotating the switch valve plate, so that liquid cannot enter the rear arm rod; the rear arm rod reciprocates to realize the pulse type water spraying operation of the spraying holes, and the spraying holes are prevented from being blocked by the materials while the materials are impacted and soaked.
Preferably, a material mixing component is arranged in the inner cylinder body and comprises a plurality of material mixing rods, the outer ends of the material mixing rods are hinged and fixed on the inner wall of the inner cylinder body, a material mixing guide cylinder is arranged at the inner end of each material mixing rod, and the inner end of each material mixing guide cylinder is open; the open end of the material mixing guide cylinder is provided with a sealing rubber ring which can elastically stretch out and draw back; a material mixing guide slot is formed at the connecting end of the material mixing guide cylinder and the material mixing rod, a material mixing guide insertion rod is arranged in the material mixing guide cylinder, one end of the material mixing guide insertion rod is inserted into the material mixing guide slot, and the other end of the material mixing guide insertion rod is hinged with a '\8834'; a circular flange guide rail is arranged on the side wall of the spraying support pipe; the guide slide block is inserted on the annular flange guide rail; a stirring rod top spring is sleeved on the stirring guide rod, the outer end of the stirring rod top spring abuts against the inner end wall of the stirring guide cylinder, and the inner end of the stirring rod top spring abuts against the guide sliding block;
when the inner cylinder body rotates, the stirring rod and the stirring guide cylinder can be driven to rotate around the spraying support pipe along the annular flange guide rail through the guide slide block for stirring; when the supporting tube driving motor is started, the spraying supporting tube moves up and down, and then the stirring guide inserting rod makes telescopic motion along the stirring guide inserting slot; in the process of mixing materials, the material mixing guide cylinder is always attached to the outer wall of the spraying support pipe through the sealing rubber ring in a sealing manner.
Preferably, a material discharge port of the water feeding mixer is connected with the stirring lubricator through a material guide pipe; the stirring lubricator and the steaming tank are sequentially arranged on the second rack from top to bottom;
the stirring and moistening device comprises a cylindrical constant-volume moistening stirring cylinder, a stirring cylinder feeding port and a stirring cylinder discharging port are respectively formed in the outer peripheral wall of the constant-volume moistening stirring cylinder, and electric switch valve bodies are respectively arranged on the stirring cylinder feeding port and the stirring cylinder discharging port; the left constant volume plate and the right constant volume plate are arranged in the constant volume material moistening stirring cylinder, a left telescopic cross rod and a right telescopic cross rod are respectively and transversely fixed on the left side and the right side of the upper part of the second rack, the left telescopic cross rod comprises a left telescopic cylinder and a left rotating shaft, the left telescopic cylinder is installed on the left upper part of the second rack, a telescopic shaft of the left telescopic cylinder is connected with the left end of the left rotating shaft in a coaxial rotatable connection mode, and the right end of the left rotating shaft is inserted into the constant volume material moistening stirring cylinder from the left side and is connected with the left end of the fixed left constant volume plate; the telescopic right cross rod comprises a telescopic right cylinder and a right rotating shaft, the telescopic shaft of the telescopic right cylinder is connected with the right end of the right rotating shaft in a coaxial rotatable connection mode, and the left end of the right rotating shaft is inserted into the constant volume moistening material stirring cylinder from the right side and is connected with the right end of the fixed right constant volume plate.
The left telescopic cylinder and the right telescopic cylinder can respectively drive the left constant volume plate and the right constant volume plate to approach or move away from each other; the peripheries of the left constant volume plate and the right constant volume plate are respectively sleeved with a left constant volume sealing ring and a right constant volume sealing ring, and the left constant volume plate and the right constant volume plate are respectively in sealing contact with the inner peripheral wall of the constant volume lubricating cylinder through the left constant volume sealing ring and the right constant volume sealing ring; left side constant volume sealing washer and right constant volume sealing washer are self-lubricating silica gel material and make.
Preferably, a left rotating shaft support arm is vertically welded and fixed at the overhanging end of the left rotating shaft, a right rotating shaft support arm is vertically welded and fixed at the overhanging end of the right rotating shaft, a support arm cross rod is fixed between the left rotating shaft support arm and the right rotating shaft support arm, a stirring cylinder rotation driving motor is fixed on the support arm cross rod, a stirring cylinder driving gear is fixed on a motor shaft of the stirring cylinder rotation driving motor, a stirring cylinder driven gear ring is sleeved on the outer peripheral wall of the constant volume material moistening stirring cylinder, and the stirring cylinder driving gear and the stirring cylinder driven gear ring are mutually meshed;
the outer peripheral wall of the constant volume moistening and stirring cylinder is respectively provided with a plurality of groups of left guide pulleys and right guide pulleys, an annular left guide hanger rail and a right guide hanger rail are sleeved outside the constant volume moistening and stirring cylinder, and the left guide pulleys and the right guide pulleys are respectively contacted with the inner walls of the left guide hanger rail and the right guide hanger rail; the upper ends of the left guide hanger rail and the right guide hanger rail are respectively fixed on the support arm cross rod;
when the stirring cylinder is started to rotate the driving motor, the stirring cylinder driving gear is meshed with the stirring cylinder driven gear ring to drive the constant-volume moistening material stirring cylinder to rotate around the left rotating shaft and the right rotating shaft in the left guide hanging rail and the right guide hanging rail to moisten materials.
Preferably, at least three groups of material stirring function groups are arranged on the left constant volume plate and the right constant volume plate, each material stirring function group comprises a first material stirring rod and a second material stirring rod, a first upper chute and a first lower chute are respectively arranged at the upper part of the right end face of the left constant volume plate and the lower part of the left end face of the right constant volume plate, a first upper slide block and a first lower slide block are respectively arranged in the first upper chute and the first lower chute, and the upper end and the lower end of the first material stirring rod are respectively hinged with the first upper slide block and the second lower slide block; a second upper sliding groove and a second lower sliding groove are respectively formed in the upper portion of the left end face of the right constant volume plate and the lower portion of the right end face of the left constant volume plate, a second upper sliding block and a second lower sliding block are respectively installed in the second upper sliding groove and the second lower sliding groove, and the upper end and the lower end of the second stirring rod are respectively hinged with the second upper sliding block and the second lower sliding block; the first stirring rod and the second stirring rod are arranged in an X shape.
Preferably, a material opening is formed in the steaming tank, and a material valve is arranged on the material opening; a left steaming material rotating shaft and a right steaming material rotating shaft are respectively fixed on the left side and the right side of the steaming material tank, the left end of the left steaming material rotating shaft is fixed on the left side of the second rack through a left bearing seat, and the right end of the right steaming material rotating shaft is fixed on the right side of the second rack through a right bearing seat; a steaming material tank turning driving motor is fixed on the left side of the second rack, a steaming material turning driving wheel is sleeved on a motor shaft of the steaming material tank turning driving motor, a steaming material turning driven wheel is sleeved on a left steaming material rotating shaft, and steaming material turning belts are sleeved on the steaming material turning driving wheel and the steaming material turning driven wheel; when the steam material tank overturning driving motor is started, the steam material tank can be driven to do 180-degree overturning motion; when the material opening of the steaming tank is upward, the material opening can correspondingly receive the discharge opening of the mixing cylinder; when the material mouth of steaming bucket was down, can realize the ejection of compact operation of unloading.
The beneficial effects are as follows:
1. the invention designs a production process of a biological microbial inoculum capable of replacing antibiotics for feeds, takes Aspergillus niger raw bacteria as a main biological microbial inoculum, and can replace antibiotic components in the traditional feeds or reduce the using amount of antibiotics. The biological agent can decompose macromolecular saccharides in the feed into monosaccharides or oligosaccharides, generate various organic acids, vitamins, biological enzymes and unknown growth factors, greatly improve the nutritional value and the digestibility of the fermented feed, and improve the animal productivity.
2. The invention can also detoxify the feed, and degrade and remove the toxic and harmful substances contained in the feed through the life activities of microorganisms, thereby improving the safety of the feed in use.
3. The invention can also improve the disease resistance of animals. The microorganisms contained in the invention directly participate in the barrier construction of animal intestinal tracts, supplement the quantity of beneficial organisms in the animal intestinal tracts, prevent the colonization and the multiplication of pathogenic microorganisms through a biological competition mechanism, and recover and maintain the microecological balance of the intestinal tracts, thereby improving the immunity and the disease resistance of animals.
4. The invention can improve the palatability of the feed, remarkably enhance the appetite, improve the utilization rate of the feed, further improve the productivity of animals, reduce the production cost and improve the breeding environment.
5. The method improves the preparation quality and yield of the biological agent and reduces the labor intensity by designing a set of modern production process and matching with special automatic equipment.
Drawings
FIG. 1 is a schematic structural diagram of a water feeding blender;
FIG. 2 is an enlarged view of portion A of FIG. 1;
FIG. 3 is an enlarged view of portion B of FIG. 1;
FIG. 4 is an enlarged view of section C of FIG. 1;
FIG. 5 is a schematic view of the mounting structure of the stirring lubricator and the steaming tank;
FIG. 6 is an enlarged view of section D of FIG. 5;
in the figure: 1. an outer cylinder; 2. a material collecting plate; 3. a first frame; 4. a water tank; 5. a flow guide pipe; 6. a lifting drive rack; 7. a rack drive gear; 8. a supporting tube driving motor; 9. a material flow guide pipe; 10. a material outlet; 11. a spraying support pipe; 12. A left guide hanger rail; 13. a material mixing component; 14. a guide pulley; 15. a guide slide rail; 16. an inner cylinder; 17. a material feeding port; 18. a material spraying and soaking mechanism; 19. the spray arm fixes the strut; 20. an inner cylinder driving motor; 21. a driving gear; 22. an intermediate transmission gear; 23. an inner gear sleeve; 24. a hollow lumen of the rear arm; 25. spraying holes; 26. a rear arm lever; 27. a forearm lever; 28. rotating the switch valve plate; 29. a hollow lumen of the forearm shaft; 30. a spherical cavity; 31. a right guide pulley; 32. A spherical joint; 33. a diversion branch pipe; 34. a material mixing rod; 35. a material mixing guide slot; 36. a material mixing guide cylinder; 37. a material mixing guide inserted bar; 38. a stirring rod top spring; 39. a guide slide block; 40. sealing the rubber ring; 41. a circular flange guide rail; 42. a left guide pulley; 43. a left rotating shaft; 44. a left rotating shaft support arm; 45. a left telescopic cylinder; 46. a second frame; 47. a left bearing seat; 48. a left steaming rotating shaft; 49. the material steaming turning driven wheel; 50. steaming and turning over a belt; 51. the steaming tank turns over the driving motor; 52. a steaming turning driving wheel; 53. steaming the material tank; 54. a right steaming material rotating shaft; 55. a right bearing seat; 56. a right rotating shaft; 57. a right telescopic cylinder; 58. a right rotating shaft support arm; 59. a right guide hanger rail; 60. a feeding port of the stirring cylinder; 61. the stirring cylinder rotates and drives the motor; 62. a driving gear of the mixing tank; 63. a passive toothed ring of the stirring cylinder; 64. a support arm cross bar; 65. a constant volume material moistening and stirring cylinder; 66. a first upper slider; 67. a first upper chute; 68. a first material stirring rod; 69. a left constant volume plate; 70. a second lower chute; 71. a second lower slider; 72. a discharge hole of the stirring cylinder; 73. a first lower slider; 74. a first lower chute; 75. a right constant volume plate; 76. a second material stirring rod; 77. a second upper chute; 78. and a second upper slide block.
Detailed Description
The present invention will be further described with reference to specific examples, which are not intended to limit the invention.
Example 1
The invention relates to a production process of a biological agent capable of replacing feed antibiotics, which comprises the following solid raw materials in percentage by weight:
80% of soybean meal;
10% of bran;
10% of corn germ meal;
the protein content of the soybean meal is 46%, and the moisture content of the bran and the corn germ meal is lower than 13%.
Preferably, the preparation process comprises the following steps:
a. preparing materials, preparing a sterilization yeast room in advance, and adjusting and checking water, electricity and equipment conditions;
b. uniformly mixing each solid raw material with water by using a water feeding and mixing device, and mixing and moistening the water for 10-20 minutes to prepare a mixed material; the mass mixing ratio of the solid raw materials to the water is 5;
c. steaming; introducing the mixed material into a rotary pressurized material steaming tank for steaming; when the material is steamed, introducing steam into the material steaming tank to steam the material; introducing steam until the pressure gauge of the steam material tank indicates 0.5Mpa, closing a steam inlet valve, opening an exhaust valve to exhaust air in the tank until the gauge pressure is 0 Mpa, closing the exhaust valve, and opening the steam inlet valve to introduce steam for steaming; when the gauge pressure is 2.0 Mpa, properly closing the steam inlet valve, keeping the pressure stable, and steaming for 30 minutes; closing the steam inlet valve, opening the exhaust valve to exhaust steam until the gauge pressure is 0 Mpa, and preparing to discharge; opening the steaming tank, rotating to a proper position with the tank opening facing downwards, matching with a crushing or conveying machine to allow the material to flow out of the steaming tank, and finally rotating the steaming tank forward and backward for 45 degrees twice to allow the material to be discharged completely; after the discharging is finished, cleaning and keeping clean, restoring the steaming tank to the original position, and turning off the operation power supply;
d. cooling and inoculating; preparing a yeast and loading the yeast into a inoculator; after the material steaming is finished, pouring the material out of the material steaming tank, sequentially starting the discharging auger, the air cooling machine and the inoculation machine, adjusting the size of a material port of the inoculation machine to a proper position, uniformly and continuously adding the yeast seeds into the inoculation auger to uniformly inoculate the clinker, and receiving the material by a material transfer vehicle and conveying the material to a yeast pool;
e. cultivating; transferring the inoculated and uniformly stirred yeast material into a yeast room, filling into a yeast pool, loosening and flattening to obtain the yeast material with the thickness of about 25cm; adjusting the temperature and the humidity of a yeast room, keeping the temperature of the materials at 30-32 ℃ and the indoor humidity at 90-95%, and carrying out heat preservation and moisture preservation culture; the early stage of aspergillus niger culture is a spore germination and hypha formation stage, aspergillus niger spores absorb water and swell to begin germination and form hypha, the growth is stable, the heat production amount is less, and heat preservation and moisture preservation are needed; when Aspergillus niger enters a rapid growth period, the koji starts to cake, proper ventilation is carried out during the period, the temperature and the humidity are frequently checked and adjusted, the temperature of the material is not more than 38 ℃, the surface of the koji does not lose water and dry, the koji turns white after about 24 hours, the surface of the fungus material starts to form spores after 36 hours, the fungus material color gradually changes from light white to black brown after entering a spore generation period, proper cooling and moisturizing are carried out during the period, the temperature of the material is controlled to be 32 to 35 ℃, the humidity is 80 to 90%, and the spores of the fungus material are dense and uniform; after 48 to 60 hours, the culture medium enters the mature period of spores, the spores basically finish forming, the spores continue to grow, the humidity in the culture medium chamber can be properly discharged, the moisture of the culture medium is gradually reduced, the spores are promoted to mature, the culture medium is dense in spore inoculation after 60 to 72 hours, the color is dark brown, the growth is uniform and thorough, the aspergillus niger is cultured to mature, and low-temperature ventilation drying can be carried out;
f. drying the yeast material; when the yeast material is cultured to be close to maturity, ventilating, dehumidifying and drying, or moving the yeast box into a drying chamber for ventilating and drying at the low temperature of 30-40 ℃, paying attention to the fact that the air temperature does not exceed 40 ℃, controlling the temperature in the drying process to be low and high, gradually raising the temperature, preventing spores from being inactivated due to insufficient heat resistance or rapid dehydration, lasting for 12-20 hours, and drying until the moisture is below 12%, wherein the yeast material is aspergillus niger raw bacteria;
g. crushing; feeding Aspergillus niger raw bacteria into a storage bin through air, crushing to obtain a fineness of 20 meshes, wherein the passing rate is 70%, and weighing and packaging after crushing.
Example two
In this embodiment, the water feeding and mixing device comprises a water feeding mixer and a stirring and moistening device.
As shown in fig. 1, the water feeding mixer comprises a first frame, an outer cylinder and an inner cylinder are fixed on the first frame, the inner cylinder is sleeved in the outer cylinder, a material feeding hole is formed in the upper end cover of the outer cylinder, and a material discharging hole is formed in the lower end cover of the outer cylinder; two ends of the inner cylinder are open.
An inner cylinder driving device is fixed on the outer cylinder and can drive the inner cylinder to rotate in the outer cylinder; the water tank is fixed on the first rack and connected with the spraying and soaking mechanism through a water pump and a flow guide pipe, and the spraying and soaking mechanism is arranged in the inner barrel body in a plug-in manner from bottom to top and can spray and soak materials in the inner barrel body.
As shown in fig. 1 and 2, the inner cylinder driving device includes an inner cylinder driving motor, a transmission gear set and an inner gear sleeve; the inner cylinder driving motor is fixed on the outer wall of the outer cylinder, and an inner gear sleeve is sleeved and fixed on the outer wall of the inner cylinder; the transmission gear set comprises a driving gear and a middle transmission gear, the driving gear is installed on a motor shaft of the inner cylinder driving motor, a transmission hole is formed in the outer wall of the outer cylinder, the middle transmission gear is installed in the transmission hole, and the driving gear is meshed with an inner gear sleeve on the inner cylinder through the middle transmission gear. The inner wall of the outer cylinder body or the inner wall of the upper end cover or the inner wall of the lower end cover is provided with an annular guide slide rail, more than three guide pulleys are annularly arranged on the outer wall of the inner cylinder body corresponding to the position of the annular guide slide rail, and each guide pulley is arranged in the guide slide rail. When the inner cylinder driving motor is started, the inner cylinder can be driven to rotate in the outer cylinder through the meshing of the transmission gear set.
As shown in fig. 1, the spraying and soaking mechanism comprises a spraying support pipe, and the spraying support pipe is inserted into the inner cylinder from bottom to top; a lifting driving rack is longitudinally fixed on the outer wall of the extending end of the spraying supporting tube, a supporting tube driving motor is fixed at the bottom of the lower end face of the outer cylinder body, a rack driving gear is fixed on a motor shaft of the supporting tube driving motor, and the rack driving gear is meshed with the lifting driving rack; the inserting end of the spraying supporting pipe is fixed with an upper spraying pipe group and a lower spraying pipe group.
As shown in fig. 3, each of the upper and lower shower groups includes at least two shower arms, and each shower arm includes a front arm rod and a rear arm rod; a front arm rod jack is formed in the pipe wall of the spraying support pipe, a spherical cavity is arranged inside the front arm rod jack, a spherical joint is arranged at the insertion end of the front arm rod, and a hollow pipe cavity is arranged in the front arm rod; the front arm rod is embedded and fixed in the spherical cavity of the front arm rod jack through a spherical joint; the flow guide pipes penetrate upwards along the spraying support pipe and are connected with the front ends of a plurality of flow guide branch pipes through multi-way joints, the rear ends of the flow guide branch pipes are communicated with an inner cavity of the spherical joint, and the inner cavity of the spherical joint is communicated with a hollow pipe cavity of the front arm rod; more than one rotary switch valve plate is arranged in the hollow tube cavity of the front arm rod through a spring articulated shaft; the inserting end of the rear arm rod is inserted into the hollow tube cavity of the front arm rod from the outward extending end of the front arm rod; the outer extending end of the rear arm rod is hinged to the lower end of the spray arm fixing support rod, and the upper end of the spray arm fixing support rod is fixedly arranged on the upper end cover or the lower end cover of the outer cylinder body; the rear arm rod is internally provided with a hollow tube cavity, the peripheral wall of the rear arm rod is provided with a plurality of spraying holes, and the spraying holes are communicated with the hollow tube cavity of the rear arm rod.
When a supporting tube driving motor is started to rotate in a forward and reverse fixed angle mode, the lifting driving rack and the spraying supporting tube can be driven to move up and down in a reciprocating mode through meshing of a rack driving gear, then each front arm rod is made to move up and down, the insertion end of the rear arm rod is made to move in a reciprocating mode along the hollow tube cavity of the front arm rod, when the rear arm rod is inserted into the innermost side of the front arm rod, the rotary switch valve plate can be pushed open, and infiltrating water enters the hollow tube cavity of the rear arm rod from the flow guide tube, the flow guide branch tube, the inner cavity of the spherical joint and the hollow tube cavity of the front arm rod and is sprayed out from the spraying holes to infiltrate materials; when the rear arm rod moves outwards, the hollow pipe cavity of the front arm rod can be automatically closed by rotating the switch valve plate, so that liquid cannot enter the rear arm rod; the rear arm rod reciprocates to realize the pulse type water spraying operation of the spraying holes, and the spraying holes are prevented from being blocked by the materials while the materials are impacted and soaked.
As shown in fig. 1 and 4, a material mixing assembly is arranged in the inner cylinder body, the material mixing assembly comprises a plurality of material mixing rods, the outer ends of the material mixing rods are hinged and fixed on the inner wall of the inner cylinder body, the inner ends of the material mixing rods are provided with material mixing guide cylinders, and the inner ends of the material mixing guide cylinders are open; an opening end of the material mixing guide cylinder is provided with a sealing rubber ring which can elastically stretch out and draw back; a material mixing guide slot is formed at the connecting end of the material mixing guide cylinder and the material mixing rod, a material mixing guide inserting rod is arranged in the material mixing guide cylinder, one end of the material mixing guide inserting rod is inserted into the material mixing guide slot, and the other end of the material mixing guide inserting rod is hinged with a '\ 8834'; a circular flange guide rail is arranged on the side wall of the spraying support pipe; the guide slide block is inserted on the annular flange guide rail; the stirring guide rod is sleeved with a stirring rod top spring, the outer end of the stirring rod top spring abuts against the inner end wall of the stirring guide cylinder, and the inner end of the stirring rod top spring abuts against the guide sliding block.
When the water feeding mixer is used, the material can be classified and quantified by the material conveying belt and slowly fed into the inner cylinder from the material feeding hole, and meanwhile, the spraying and soaking mechanism is started, so that the soaking liquid is moved in the inner cylinder to be sprayed, and the wetting is realized by contacting water in the falling process of the material. The inner cylinder body driving motor is started while feeding, the inner cylinder body can be driven to rotate in the outer cylinder body through the meshing of the transmission gear set, and the inner cylinder body can drive the material mixing rod and the material mixing guide cylinder to rotate around the spraying support tube along the annular flange guide rail through the guide slide block for mixing materials; when the supporting tube driving motor is started, the spraying supporting tube moves up and down, and then the stirring guide inserting rod makes telescopic motion along the stirring guide inserting slot; in the material mixing process, the material mixing guide cylinder is always sealed and attached to the outer wall of the spraying support pipe through the sealing rubber ring. After the intensive mixing material, open the material discharge gate, through installing the material collecting plate on interior barrel inside wall, with the rotatory propelling movement to the material exit of collecting of material, again in the same direction as leading-in moist material ware of stirring of material through the material honeycomb duct by the material discharge gate.
The water feeding blender can realize spray infiltration at multiple levels and multiple angles in synchronization in the process of feeding materials, and mix the material component by rotating the inner cylinder and driving the inner cylinder, and mix the materials and the infiltration liquid in a rotating and swinging manner, so that the situation of accumulation of the materials such as lumps is prevented.
As shown in fig. 5, the stirring lubricator and the steaming tank are sequentially installed on the second rack from top to bottom. The stirring and moistening device comprises a cylindrical constant-volume moistening stirring cylinder, a stirring cylinder feeding port and a stirring cylinder discharging port are respectively formed in the outer peripheral wall of the constant-volume moistening stirring cylinder, and electric switch valve bodies are respectively arranged on the stirring cylinder feeding port and the stirring cylinder discharging port; the constant-volume moistening stirring device is characterized in that a left constant-volume plate and a right constant-volume plate are arranged in a constant-volume moistening stirring cylinder, a left telescopic cross rod and a right telescopic cross rod are transversely fixed on the left side and the right side of the upper portion of a second rack respectively, the left telescopic cross rod comprises a left telescopic cylinder and a left rotating shaft, the left telescopic cylinder is installed on the left upper portion of the second rack, a telescopic shaft of the left telescopic cylinder is connected with the left end of the left rotating shaft in a coaxial rotatable connection mode, and the right end of the left rotating shaft is inserted into the constant-volume moistening stirring cylinder from the left side and is connected with the left end of the fixed left constant-volume plate; the telescopic right cross rod comprises a telescopic right cylinder and a right rotating shaft, the telescopic shaft of the telescopic right cylinder is connected with the right end of the right rotating shaft in a coaxial rotatable connection mode, and the left end of the right rotating shaft is inserted into the constant volume moistening material stirring cylinder from the right side and is connected with the right end of the fixed right constant volume plate.
As shown in fig. 6, the left telescopic cylinder and the right telescopic cylinder can respectively drive the left constant volume plate and the right constant volume plate to approach or separate from each other; the periphery of the left constant volume plate and the periphery of the right constant volume plate are respectively sleeved with a left constant volume sealing ring and a right constant volume sealing ring, and the left constant volume plate and the right constant volume plate are respectively in sealing contact with the inner peripheral wall of the constant volume lubricating cylinder through the left constant volume sealing ring and the right constant volume sealing ring; left side constant volume sealing washer and right constant volume sealing washer are self-lubricating silica gel material and make.
The stirring device comprises a left rotating shaft, a right rotating shaft, a left rotating shaft supporting arm, a supporting arm cross rod, a stirring cylinder rotation driving motor, a stirring cylinder driving gear, a stirring cylinder driven gear ring and a stirring cylinder driven gear ring, wherein the left rotating shaft supporting arm is vertically welded and fixed at the overhanging end of the left rotating shaft, the right rotating shaft supporting arm is vertically welded and fixed at the overhanging end of the right rotating shaft, the supporting arm cross rod is fixed between the left rotating shaft supporting arm and the right rotating shaft supporting arm, the stirring cylinder rotation driving motor is fixed on the supporting arm cross rod, the stirring cylinder driving gear is fixed on a motor shaft of the stirring cylinder rotation driving motor, the stirring cylinder driven gear ring is sleeved on the outer peripheral wall of the constant-volume material moistening stirring cylinder, and the stirring cylinder driving gear and the stirring cylinder driven gear ring are meshed with each other.
The outer peripheral wall of the constant volume moistening and stirring cylinder is respectively provided with a plurality of groups of left guide pulleys and right guide pulleys, an annular left guide hanger rail and a right guide hanger rail are sleeved outside the constant volume moistening and stirring cylinder, and the left guide pulleys and the right guide pulleys are respectively contacted with the inner walls of the left guide hanger rail and the right guide hanger rail; the upper ends of the left guide hanger rail and the right guide hanger rail are respectively fixed on the support arm cross rod.
When the stirring cylinder is started to rotate the driving motor, the stirring cylinder driving gear is meshed with the stirring cylinder driven gear ring to drive the constant-volume material moistening stirring cylinder to rotate around the left rotating shaft and the right rotating shaft in the left guide hanging rail and the right guide hanging rail to moisten materials.
As shown in fig. 6, at least three groups of material stirring function groups are installed on the left constant volume plate and the right constant volume plate, each material stirring function group comprises a first material stirring rod and a second material stirring rod, a first upper chute and a first lower chute are respectively formed in the upper portion of the right end face of the left constant volume plate and the lower portion of the left end face of the right constant volume plate, a first upper sliding block and a first lower sliding block are respectively installed in the first upper chute and the first lower chute, and the upper end and the lower end of the first material stirring rod are respectively hinged to the first upper sliding block and the second lower sliding block; a second upper sliding groove and a second lower sliding groove are respectively formed in the upper portion of the left end face of the right constant volume plate and the lower portion of the right end face of the left constant volume plate, a second upper sliding block and a second lower sliding block are respectively installed in the second upper sliding groove and the second lower sliding groove, and the upper end and the lower end of a second material stirring rod are respectively hinged with the second upper sliding block and the second lower sliding block; the first stirring rod and the second stirring rod are arranged in an X shape.
When the stirring lubricator is used, the distance between the left constant volume plate and the right constant volume plate is firstly adjusted to achieve the purpose of adjusting the internal volume of the constant volume lubricator stirring cylinder. And then opening an electric switch valve body on a feeding port of the mixing tank, enabling the solid-liquid mixture to enter the constant-volume moistening material mixing tank along a material flow guide pipe, closing the electric switch valve body on the feeding port of the mixing tank, and sealing the constant-volume moistening material mixing tank. And starting the stirring cylinder to rotate the driving motor, wherein the driving gear of the stirring cylinder is meshed with the driven gear ring of the stirring cylinder to drive the constant-volume material moistening stirring cylinder to rotate and moisten materials around the left rotating shaft and the right rotating shaft in the left guide hanger rail and the right guide hanger rail. Meanwhile, relative motion occurs between the material stirring functional group and the materials, and the materials are cut and stirred, so that the material stirring and moistening are more uniform. After the stirring is preset for a long time, the left telescopic cylinder and the right telescopic cylinder are started, the left constant volume plate and the right constant volume plate move relatively, mixed materials between the left constant volume plate and the right constant volume plate are moderately pressurized, liquid is pressed into solid materials through pressure, the infiltration time is shortened, and the saturation rate is increased.
As shown in fig. 5, a material opening is formed on the steaming tank, and a material valve is arranged on the material opening; a left steaming material rotating shaft and a right steaming material rotating shaft are respectively fixed on the left side and the right side of the steaming material tank, the left end of the left steaming material rotating shaft is fixed on the left side of the second rack through a left bearing seat, and the right end of the right steaming material rotating shaft is fixed on the right side of the second rack through a right bearing seat; a steaming tank overturning driving motor is fixed on the left side of the second rack, a steaming material overturning driving wheel is sleeved on a motor shaft of the steaming tank overturning driving motor, a steaming material overturning driven wheel is sleeved on a left steaming material rotating shaft, and a steaming material overturning belt is sleeved on the steaming material overturning driving wheel and the steaming material overturning driven wheel; when the steam tank turnover driving motor is started, the steam tank can be driven to do 180-degree turnover movement; when the material opening of the steaming tank is upward, the material opening can correspondingly receive the discharge opening of the stirring cylinder; when the material mouth of the steaming tank faces downwards, the discharging and unloading operation can be realized.
The invention makes innovative improvement on the equipment for soaking, stirring and steaming materials in the early stage, can realize automation of links with high labor intensity, reduces the labor intensity of workers to the maximum extent, improves the operation efficiency and improves the pretreatment quality of the materials. Other related devices such as a discharging auger, an air cooling machine, a inoculation machine and the like, and facilities such as a yeast room, a yeast pool and the like are mature prior art, are not main innovation points of the patent, and are not repeated for specific structures and using methods.
The foregoing is directed to preferred embodiments of the present invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof, and the scope thereof is determined by the claims that follow. Any simple modification, equivalent change and modification of the above embodiments according to the technical essence of the present invention will still fall within the protection scope of the technical solution of the present invention.

Claims (6)

1. A production process of a biological agent capable of replacing antibiotics for feeds is characterized in that the biological agent capable of replacing the antibiotics for the feeds comprises solid raw materials prepared from the following components in percentage by weight:
80% of soybean meal;
10 percent of bran;
10% of corn germ meal;
the protein content of the soybean meal is 46%, and the moisture content of the bran and the corn germ meal is lower than 13%;
the production process comprises the following steps:
a. preparing materials, preparing a sterilization yeast room in advance, and adjusting and checking water, electricity and equipment conditions;
b. uniformly mixing each solid raw material with water by using a water feeding and mixing device, and mixing and moistening the water for 10-20 minutes to prepare a mixed material; the mass mixing ratio of the solid raw material to water is 5;
c. steaming; introducing the mixed material into a material steaming tank, and steaming; when the material is steamed, introducing steam into the material steaming tank to steam the material; introducing steam until the indication of a pressure gauge of the steam material tank is 0.5Mpa, closing a steam inlet valve, opening an exhaust valve to exhaust air in the tank until the gauge pressure is 0 Mpa, closing the exhaust valve, and opening the steam inlet valve to introduce steam to steam the materials; when the gauge pressure is 2.0 Mpa, properly closing the steam inlet valve, keeping the pressure stable, and steaming for 30 minutes; closing the steam inlet valve, opening the exhaust valve to exhaust steam until the gauge pressure is 0 Mpa, and preparing to discharge; opening the steaming tank, rotating to a proper position with the tank opening facing downwards, matching with a crushing or conveying machine to allow the material to flow out of the steaming tank, and finally rotating the steaming tank forward and backward for 45 degrees twice to allow the material to be discharged completely; after the discharging is finished, cleaning and keeping clean, restoring the steaming tank to the original position, and turning off the operation power supply;
d. cooling and inoculating; preparing a yeast and loading into a inoculator; after the material steaming is finished, pouring the material out of the material steaming tank, sequentially starting the discharging auger, the air cooling machine and the inoculation machine, adjusting the size of a material port of the inoculation machine to a proper position, uniformly and continuously feeding the yeast seeds into the inoculation auger to uniformly inoculate clinker, and receiving the material by a material transfer vehicle and conveying the material to a yeast pond;
e. cultivating; transferring the inoculated and uniformly stirred yeast material into a yeast room, filling into a yeast pool, loosening and flattening, wherein the thickness is 25cm; adjusting the temperature and the humidity of a yeast room, keeping the temperature of the materials at 30-32 ℃ and the indoor humidity at 90-95%, and carrying out heat preservation and moisture preservation culture; the early stage of aspergillus niger culture is a spore germination and hypha formation stage, aspergillus niger spores absorb water and swell to begin germination and form hypha, the growth is stable, the heat production amount is less, and heat preservation and moisture preservation are needed; when Aspergillus niger enters a rapid growth period, the koji starts to cake, proper ventilation is carried out during the period, the temperature and the humidity are frequently checked and adjusted, the temperature of the material is not more than 38 ℃, the surface of the koji does not lose water and dry, the koji turns white after about 24 hours, the surface of the fungus material starts to form spores after 36 hours, the fungus material color gradually changes from light white to black brown after entering a spore generation period, proper cooling and moisturizing are carried out during the period, the temperature of the material is controlled to be 32 to 35 ℃, the humidity is 80 to 90%, and the spores of the fungus material are dense and uniform; after 48 to 60 hours, the culture medium enters the mature period of spores, the spores are basically finished, the spores continue to grow, the humidity in the culture medium chamber is properly discharged, the moisture of the culture medium is gradually reduced, the spores are promoted to mature, the culture medium is dense in spore inoculation after 60 to 72 hours, the color is dark brown, the growth is uniform and thorough, the aspergillus niger is cultured to mature, and low-temperature ventilation drying is carried out;
f. drying the yeast material; when the yeast material is cultured to be close to maturity, ventilation, moisture removal and drying are carried out, or the yeast box is moved into a drying chamber to be ventilated and dried under the condition of low temperature of 30-40 ℃, the air temperature is not higher than 40 ℃, the temperature is controlled to be low firstly and then high in the drying process, the temperature is gradually increased, the spores are prevented from being inactivated due to insufficient heat resistance or rapid dehydration, the duration time is 12-20 hours, and the spores are dried until the moisture is below 12%, namely the aspergillus niger protobacteria;
g. crushing; feeding Aspergillus niger raw bacteria into a storage bin through air, crushing to obtain 20-mesh pass rate of 70%, and weighing and packaging after crushing;
the water feeding and mixing device comprises a water feeding mixer and a stirring and moistening device;
the water feeding mixer comprises a first frame, an outer cylinder and an inner cylinder are fixed on the first frame, the inner cylinder is sleeved in the outer cylinder, a material feeding hole is formed in the upper end cover of the outer cylinder, and a material discharging hole is formed in the lower end cover of the outer cylinder; two ends of the inner cylinder are open;
an inner cylinder driving device is fixed on the outer cylinder and can drive the inner cylinder to rotate in the outer cylinder;
a water tank is fixed on the first frame, the water tank is connected with a spraying and soaking mechanism through a water pump and a flow guide pipe, the spraying and soaking mechanism is inserted into the inner cylinder from bottom to top and can spray and soak materials in the inner cylinder;
the inner cylinder driving device comprises an inner cylinder driving motor, a transmission gear set and an inner gear sleeve; the inner cylinder driving motor is fixed on the outer wall of the outer cylinder, and an inner gear sleeve is sleeved and fixed on the outer wall of the inner cylinder; the transmission gear set comprises a driving gear and a middle transmission gear, the driving gear is installed on a motor shaft of the driving motor of the inner cylinder, a transmission hole is formed in the outer wall of the outer cylinder, the middle transmission gear is installed in the transmission hole, and the driving gear is meshed with an inner gear sleeve on the inner cylinder through the middle transmission gear;
an annular guide sliding rail is arranged on the inner wall of the outer cylinder body or the inner wall of the upper end cover or the inner wall of the lower end cover, more than three guide pulleys are annularly arranged on the outer wall of the inner cylinder body corresponding to the position of the annular guide sliding rail, and each guide pulley is arranged in the guide sliding rail;
when the driving motor of the inner cylinder body is started, the inner cylinder body can be driven to rotate in the outer cylinder body through the meshing of the transmission gear set;
the spraying and soaking mechanism comprises a spraying support pipe, and the spraying support pipe is inserted into the inner cylinder from bottom to top; a lifting driving rack is longitudinally fixed on the outer wall of the extending end of the spraying supporting tube, a supporting tube driving motor is fixed at the bottom of the lower end face of the outer cylinder body, a rack driving gear is fixed on a motor shaft of the supporting tube driving motor, and the rack driving gear is meshed with the lifting driving rack;
an upper spraying pipe group and a lower spraying pipe group are fixed at the insertion end of the spraying supporting pipe;
the upper spraying pipe group and the lower spraying pipe group respectively comprise at least two spraying arms, and each spraying arm comprises a front arm rod and a rear arm rod; a front arm rod jack is formed in the pipe wall of the spraying support pipe, a spherical cavity is arranged inside the front arm rod jack, a spherical joint is arranged at the insertion end of the front arm rod, and a hollow pipe cavity is arranged in the front arm rod; the front arm rod is embedded and fixed in the spherical cavity of the front arm rod jack through a spherical joint; the flow guide pipes penetrate upwards along the spraying support pipe and are connected with the front ends of a plurality of flow guide branch pipes through multi-way joints, the rear ends of the flow guide branch pipes are communicated with an inner cavity of the spherical joint, and the inner cavity of the spherical joint is communicated with a hollow pipe cavity of the front arm rod; more than one rotary switch valve plate is arranged in the hollow tube cavity of the front arm rod through a spring articulated shaft; the inserting end of the rear arm rod is inserted into the hollow tube cavity of the front arm rod from the outward extending end of the front arm rod; the outer extending end of the rear arm rod is hinged to the lower end of the spray arm fixing support rod, and the upper end of the spray arm fixing support rod is fixedly arranged on the upper end cover or the lower end cover of the outer cylinder body; a hollow tube cavity is arranged in the rear arm rod, a plurality of spraying holes are formed in the outer peripheral wall of the rear arm rod, and the spraying holes are communicated with the hollow tube cavity of the rear arm rod;
when a supporting tube driving motor is started to rotate in a forward and reverse fixed angle mode, the lifting driving rack and the spraying supporting tube can be driven to reciprocate up and down through meshing of a rack driving gear, then each front arm rod is enabled to move up and down, the insertion end of the rear arm rod is enabled to reciprocate along the hollow tube cavity of the front arm rod, when the rear arm rod is inserted into the innermost side of the front arm rod, the rotary switch valve plate can be pushed open, and infiltrating water can enter the hollow tube cavity of the rear arm rod from the flow guide tube, the flow guide branch tube, the inner cavity of the spherical joint and the hollow tube cavity of the front arm rod and can be sprayed out from the spraying holes to infiltrate materials; when the rear arm rod moves outwards, the hollow tube cavity of the front arm rod can be automatically closed by rotating the switch valve plate, so that liquid cannot enter the rear arm rod; the rear arm rod reciprocates to realize the pulse type water spraying operation of the spraying holes.
2. The process for producing the biological agent capable of replacing antibiotics for feed according to claim 1, wherein the biological agent comprises the following components: a material mixing component is arranged in the inner cylinder body and comprises a plurality of material mixing rods, the outer ends of the material mixing rods are hinged and fixed on the inner wall of the inner cylinder body, the inner ends of the material mixing rods are provided with material mixing guide cylinders, and the inner ends of the material mixing guide cylinders are open; an opening end of the material mixing guide cylinder is provided with a sealing rubber ring which can elastically stretch out and draw back; a material mixing guide slot is formed at the connecting end of the material mixing guide cylinder and the material mixing rod, a material mixing guide inserting rod is arranged in the material mixing guide cylinder, one end of the material mixing guide inserting rod is inserted into the material mixing guide slot, and the other end of the material mixing guide inserting rod is hinged with a '\ 8834'; a circular flange guide rail is arranged on the side wall of the spraying support pipe; the guide slide block is inserted on the annular flange guide rail; a stirring rod top spring is sleeved on the stirring guide rod, the outer end of the stirring rod top spring abuts against the inner end wall of the stirring guide cylinder, and the inner end of the stirring rod top spring abuts against the guide sliding block;
when the inner cylinder body rotates, the stirring rod and the stirring guide cylinder can be driven to rotate around the spraying support pipe along the annular flange guide rail through the guide slide block for stirring; when the supporting tube driving motor is started, the spraying supporting tube moves up and down, and then the stirring guide inserting rod makes telescopic motion along the stirring guide inserting slot; in the process of mixing materials, the material mixing guide cylinder is always attached to the outer wall of the spraying support pipe through the sealing rubber ring in a sealing manner.
3. The process for producing the biological agent capable of replacing antibiotics for feeds as claimed in claim 2, wherein the biological agent comprises: a material discharge port of the water feeding mixer is connected with the stirring material wetting device through a material guide pipe; the stirring material wetting device and the steaming tank are sequentially arranged on the second rack from top to bottom;
the stirring and moistening device comprises a cylindrical constant-volume moistening stirring cylinder, a stirring cylinder feeding port and a stirring cylinder discharging port are respectively formed in the outer peripheral wall of the constant-volume moistening stirring cylinder, and electric switch valve bodies are respectively arranged on the stirring cylinder feeding port and the stirring cylinder discharging port; the left constant volume plate and the right constant volume plate are arranged in the constant volume material moistening stirring cylinder, a left telescopic cross rod and a right telescopic cross rod are respectively and transversely fixed on the left side and the right side of the upper part of the second rack, the left telescopic cross rod comprises a left telescopic cylinder and a left rotating shaft, the left telescopic cylinder is installed on the left upper part of the second rack, a telescopic shaft of the left telescopic cylinder is connected with the left end of the left rotating shaft in a coaxial rotatable connection mode, and the right end of the left rotating shaft is inserted into the constant volume material moistening stirring cylinder from the left side and is connected with the left end of the fixed left constant volume plate; the right telescopic cross rod comprises a right telescopic cylinder and a right rotating shaft, the telescopic shaft of the right telescopic cylinder is connected with the right end of the right rotating shaft in a coaxial rotatable connection mode, and the left end of the right rotating shaft is inserted into the constant volume material moistening and stirring cylinder from the right side and is connected with and fixes the right end of the right constant volume plate;
the left telescopic cylinder and the right telescopic cylinder can respectively drive the left constant volume plate and the right constant volume plate to approach or separate from each other; the peripheries of the left constant volume plate and the right constant volume plate are respectively sleeved with a left constant volume sealing ring and a right constant volume sealing ring, and the left constant volume plate and the right constant volume plate are respectively in sealing contact with the inner peripheral wall of the constant volume lubricating cylinder through the left constant volume sealing ring and the right constant volume sealing ring; left side constant volume sealing washer and right constant volume sealing washer are self-lubricating silica gel material and make.
4. The process for producing the biological agent capable of replacing antibiotics for feeds as claimed in claim 3, wherein the biological agent comprises: a left rotating shaft support arm is vertically welded and fixed at the overhanging end of the left rotating shaft, a right rotating shaft support arm is vertically welded and fixed at the overhanging end of the right rotating shaft, a support arm cross rod is fixed between the left rotating shaft support arm and the right rotating shaft support arm, a stirring cylinder rotation driving motor is fixed on the support arm cross rod, a stirring cylinder driving gear is fixed on a motor shaft of the stirring cylinder rotation driving motor, a stirring cylinder driven gear ring is sleeved on the outer peripheral wall of the constant volume material moistening stirring cylinder, and the stirring cylinder driving gear and the stirring cylinder driven gear ring are mutually meshed;
the outer peripheral wall of the constant volume material moistening and stirring cylinder is respectively provided with a plurality of groups of left guide pulleys and right guide pulleys, the outer part of the constant volume material moistening and stirring cylinder is sleeved with a ring-shaped left guide hanging rail and a ring-shaped right guide hanging rail, and the left guide pulleys and the right guide pulleys are respectively contacted with the inner walls of the left guide hanging rail and the right guide hanging rail; the upper ends of the left guide hanger rail and the right guide hanger rail are respectively fixed on the support arm cross rod;
when the stirring cylinder is started to rotate the driving motor, the stirring cylinder driving gear is meshed with the stirring cylinder driven gear ring to drive the constant-volume moistening material stirring cylinder to rotate around the left rotating shaft and the right rotating shaft in the left guide hanging rail and the right guide hanging rail to moisten materials.
5. The process for producing the biological agent capable of replacing antibiotics for feed according to claim 4, wherein the biological agent comprises the following components: at least three groups of stirring function groups are arranged on the left constant volume plate and the right constant volume plate, each stirring function group comprises a first stirring rod and a second stirring rod, a first upper chute and a first lower chute are respectively arranged at the upper part of the right end face of the left constant volume plate and the lower part of the left end face of the right constant volume plate, a first upper sliding block and a first lower sliding block are respectively arranged in the first upper chute and the first lower chute, and the upper end and the lower end of the first stirring rod are respectively hinged with the first upper sliding block and the second lower sliding block; a second upper sliding groove and a second lower sliding groove are respectively formed in the upper portion of the left end face of the right constant volume plate and the lower portion of the right end face of the left constant volume plate, a second upper sliding block and a second lower sliding block are respectively installed in the second upper sliding groove and the second lower sliding groove, and the upper end and the lower end of the second stirring rod are respectively hinged with the second upper sliding block and the second lower sliding block; the first stirring rod and the second stirring rod are arranged in an X shape.
6. The process for producing the biological agent capable of replacing antibiotics for feed according to claim 5, wherein the biological agent comprises the following components: a material opening is formed in the steaming tank, and a material valve is arranged on the material opening; a left steaming material rotating shaft and a right steaming material rotating shaft are respectively fixed on the left side and the right side of the steaming material tank, the left end of the left steaming material rotating shaft is fixed on the left side of the second rack through a left bearing seat, and the right end of the right steaming material rotating shaft is fixed on the right side of the second rack through a right bearing seat; a steaming tank overturning driving motor is fixed on the left side of the second rack, a steaming material overturning driving wheel is sleeved on a motor shaft of the steaming tank overturning driving motor, a steaming material overturning driven wheel is sleeved on a left steaming material rotating shaft, and a steaming material overturning belt is sleeved on the steaming material overturning driving wheel and the steaming material overturning driven wheel; when the steam material tank overturning driving motor is started, the steam material tank can be driven to do 180-degree overturning motion; when the material opening of the steaming tank is upward, the material opening can correspondingly receive the discharge opening of the mixing cylinder; when the material mouth of the steaming tank faces downwards, the discharging and unloading operation can be realized.
CN202110164774.8A 2021-02-06 2021-02-06 Production process of biological agent capable of replacing antibiotics for feed Active CN112725196B (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1899080A (en) * 2005-07-19 2007-01-24 李同军 Active protein feed and its preparing method
CN105166416A (en) * 2015-10-13 2015-12-23 武汉新兴地生物技术发展有限公司 Compound microorganism feed additive and preparation method thereof
CN105941828A (en) * 2016-05-16 2016-09-21 内蒙古工业大学 Method for producing compound feed from mixed strains through united fermentation
CN112167433A (en) * 2020-10-09 2021-01-05 安徽省金裕生物科技开发有限公司 High-protein distiller's grain feed and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1899080A (en) * 2005-07-19 2007-01-24 李同军 Active protein feed and its preparing method
CN105166416A (en) * 2015-10-13 2015-12-23 武汉新兴地生物技术发展有限公司 Compound microorganism feed additive and preparation method thereof
CN105941828A (en) * 2016-05-16 2016-09-21 内蒙古工业大学 Method for producing compound feed from mixed strains through united fermentation
CN112167433A (en) * 2020-10-09 2021-01-05 安徽省金裕生物科技开发有限公司 High-protein distiller's grain feed and preparation method thereof

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