CN103564153A - Method for converting non-protein nitrogen into mycoprotein feed - Google Patents
Method for converting non-protein nitrogen into mycoprotein feed Download PDFInfo
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- CN103564153A CN103564153A CN201210256355.8A CN201210256355A CN103564153A CN 103564153 A CN103564153 A CN 103564153A CN 201210256355 A CN201210256355 A CN 201210256355A CN 103564153 A CN103564153 A CN 103564153A
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- mycoprotein
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- candida utili
- urea
- ammonium sulfate
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Abstract
A method for converting non-protein nitrogen into mycoprotein feed relates to a method of mycoprotein feeds, and specifically relates to a method for converting non-protein nitrogen into mycoprotein feed. The method comprises: taking wort, wheat bran, rice bran meal, urea, ammonium sulfate and candida utilis as raw materials, converting urea and ammonium sulfate into mycoprotein under the fermentation effect of candida utilis. The method provided by the invention not only helps to solve the resource shortage problem of protein feeds, but also helps to improve culture efficiency and promote development of animal husbandry.
Description
(1), technical field
What the present invention narrated is a kind of production method of animal feeding-stuff containing somatic protein, specifically nonprotein nitrogen is changed into the method for animal feeding-stuff containing somatic protein.
(2), background technology
Mycoprotein is again microprotein, single cell protein, its contained nutriment is very abundant, mycoprotein very easily decomposes and absorbs in animal body, nutritive validity is obviously better than puffed soybean, the amino acid whose composition of mycoprotein is comparatively complete, comprises the less lysine of content in cereal, also contains multivitamin, carbohydrate, lipid, mineral matter in mycoprotein, and abundant enzyme and bioactivator, as coacetylase, ubiquinone, glutathione, ergosterol etc.Mycoprotein is added in feed by a certain percentage, can improve the utilization rate of feed, promote the growth of animal, improve the disease-resistant and immunocompetence of domestic birds and animals, improve the output of meat, eggs and milk, increase culture benefit.
At present, protein feed resource shortage is one of subject matter of facing of China's animal husbandry and feed industrial development, utilizes microorganism that nonprotein nitrogen is changed into animal feeding-stuff containing somatic protein, both can solve the problem of protein feed resource shortage, improve again cultivation efficiency, promote the development of animal husbandry.
(3), summary of the invention
The object of the invention is to for the deficiency of protein feed resource shortage and the preparation method of the high-quality animal feeding-stuff containing somatic protein providing.
The object of the present invention is achieved like this: first prepare candida utili liquid seeds, then by the fermentation of candida utili, nonprotein nitrogen is changed into animal feeding-stuff containing somatic protein.
(1) preparation of candida utili liquid seeds: by malt extract medium sterilizing 15～20 minutes at 121 ℃, be cooled to 28～30 ℃, inoculation candida utili is cultivated 16～20 hours under 28～32 ℃, the condition of rotary shaker 150rpm.
(2) nonprotein nitrogen is changed into animal feeding-stuff containing somatic protein: take 85 grams, wheat bran, 9 grams of rice bran meals, 4.5 grams, urea, 1.5 grams, ammonium sulfate, add the water of 1.1 times of mixed material gross weights to mix, at 121 ℃, sterilizing is 30～40 minutes, is cooled to 28～30 ℃, accesses 10% candida utili liquid seeds, mix thoroughly, tiling is cultivated to putting into insulating box on tray, 30 ℃ of cultivation temperature, incubation time 68～72 hours.After fermentation ends, under 42～45 ℃ of conditions, low temperature is drying to obtain finished product.
The present invention changes into mycoprotein by the fermentation of candida utili by the nonprotein nitrogen in urea, ammonium sulfate, and technique is simple, with low cost.In batching, wheat bran and rice bran meal mix rear protein content 15～18%, and after candida utili fermentation, protein content can reach 28～30%, and the protein of raising is all the mycoprotein that urea and ammonium sulfate change into.In feed, add 3-5% mycoprotein, equivalent substitution soya-bean cake, peanut cake fish meal can be obtained the effect of volume increase 4-12%.
The mycoprotein that the present invention is produced adds in feed in 3-5% ratio, can improve the utilization rate of feed, promotes the growth of animal, improves the disease-resistant and immunocompetence of domestic birds and animals, improves the output of meat, eggs and milk, increases culture benefit.
(4), specific embodiments
(1) preparation of candida utili liquid seeds: by the sterilizing 15～20 minutes at 121 ℃ of 100 milliliters of malt extract mediums, be cooled to 28～30 ℃, from inclined-plane, inoculate a ring candida utili, under 28～32 ℃, the condition of rotary shaker 150rpm, cultivate 16～20 hours.
(2) nonprotein nitrogen is changed into feeding mycoprotein: take 85 grams, wheat bran, 9 grams of rice bran meals, 4.5 grams, urea, 1.5 grams, ammonium sulfate, add 110 grams of water to mix, at 121 ℃, sterilizing is 35 minutes, is cooled to 30 ℃, the candida utili liquid seeds of access 10ml, mix thoroughly, tiling is cultivated to putting into insulating box on tray, 30 ℃ of cultivation temperature, incubation time 72 hours.After fermentation ends, under 45 ℃ of conditions, low temperature is drying to obtain finished product.
Claims (1)
1. nonprotein nitrogen is changed into a method for animal feeding-stuff containing somatic protein, it is characterized in that take that brewer's wort, wheat bran, rice bran meal, urea, ammonium sulfate, candida utili are raw material, through following machining process, be prepared from:
(1) preparation of candida utili liquid seeds: by malt extract medium sterilizing 15～20 minutes at 121 ℃, be cooled to 28～30 ℃, inoculation candida utili is cultivated 16～20 hours under 28～32 ℃, the condition of rotary shaker 150rpm;
(2) nonprotein nitrogen is changed into feeding mycoprotein: take 85 grams, wheat bran, 9 grams of rice bran meals, 4.5 grams, urea, 1.5 grams, ammonium sulfate, add the water of 1.1 times of mixed material gross weights to mix, at 121 ℃, sterilizing is 30～40 minutes, is cooled to 28～30 ℃, accesses 10% candida utili liquid seeds, mix thoroughly, tiling is cultivated to putting into insulating box on tray, 30 ℃ of cultivation temperature, incubation time 68～72 hours.After fermentation ends, under 42～45 ℃ of conditions, low temperature is drying to obtain finished product.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210256355.8A CN103564153A (en) | 2012-07-24 | 2012-07-24 | Method for converting non-protein nitrogen into mycoprotein feed |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210256355.8A CN103564153A (en) | 2012-07-24 | 2012-07-24 | Method for converting non-protein nitrogen into mycoprotein feed |
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| CN103564153A true CN103564153A (en) | 2014-02-12 |
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| CN201210256355.8A Pending CN103564153A (en) | 2012-07-24 | 2012-07-24 | Method for converting non-protein nitrogen into mycoprotein feed |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108094678A (en) * | 2017-12-08 | 2018-06-01 | 重庆昇顺科技有限公司 | A kind of Wheat Brag Protein cures fermented feed preparation method |
| CN109007257A (en) * | 2018-08-02 | 2018-12-18 | 北京首钢朗泽新能源科技有限公司 | A kind of animal feeding-stuff containing somatic protein, production technology and its application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1470181A (en) * | 2003-04-24 | 2004-01-28 | 西北农林科技大学 | Method for producing protein feed by using apple residue double-baterial fermentation |
| CN1899080A (en) * | 2005-07-19 | 2007-01-24 | 李同军 | Active protein feed and its preparing method |
| CN102178038A (en) * | 2011-04-22 | 2011-09-14 | 河南宏翔生物科技有限公司 | Method for preparing fermented high-lysine high-protein feed |
-
2012
- 2012-07-24 CN CN201210256355.8A patent/CN103564153A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1470181A (en) * | 2003-04-24 | 2004-01-28 | 西北农林科技大学 | Method for producing protein feed by using apple residue double-baterial fermentation |
| CN1899080A (en) * | 2005-07-19 | 2007-01-24 | 李同军 | Active protein feed and its preparing method |
| CN102178038A (en) * | 2011-04-22 | 2011-09-14 | 河南宏翔生物科技有限公司 | Method for preparing fermented high-lysine high-protein feed |
Non-Patent Citations (2)
| Title |
|---|
| 徐姗楠等: "微生物发酵生产蛋白饲料的研究进展", 《福州大学学报(自然科学版)》 * |
| 赵蕾等: "夏橙皮渣产单细胞蛋白菌种筛选和发酵条件的优化", 《食品与发酵工业》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108094678A (en) * | 2017-12-08 | 2018-06-01 | 重庆昇顺科技有限公司 | A kind of Wheat Brag Protein cures fermented feed preparation method |
| CN109007257A (en) * | 2018-08-02 | 2018-12-18 | 北京首钢朗泽新能源科技有限公司 | A kind of animal feeding-stuff containing somatic protein, production technology and its application |
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Application publication date: 20140212 |