CN108893406A - Enzyme microb quantifies production system and method - Google Patents
Enzyme microb quantifies production system and method Download PDFInfo
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 56
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 36
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 18
- 238000000855 fermentation Methods 0.000 claims abstract description 109
- 230000004151 fermentation Effects 0.000 claims abstract description 105
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 66
- 238000003860 storage Methods 0.000 claims abstract description 38
- 238000013139 quantization Methods 0.000 claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 89
- 239000000463 material Substances 0.000 claims description 83
- 239000001963 growth medium Substances 0.000 claims description 75
- 230000001954 sterilising effect Effects 0.000 claims description 47
- 238000003756 stirring Methods 0.000 claims description 30
- 238000010438 heat treatment Methods 0.000 claims description 29
- 238000011084 recovery Methods 0.000 claims description 18
- 239000002994 raw material Substances 0.000 claims description 17
- 238000004090 dissolution Methods 0.000 claims description 16
- 230000003519 ventilatory effect Effects 0.000 claims description 12
- 238000007599 discharging Methods 0.000 claims description 11
- 239000004615 ingredient Substances 0.000 claims description 11
- 239000011229 interlayer Substances 0.000 claims description 10
- 230000000813 microbial effect Effects 0.000 claims description 10
- 238000010792 warming Methods 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 8
- 238000012856 packing Methods 0.000 claims description 7
- 239000010410 layer Substances 0.000 claims description 6
- 238000004321 preservation Methods 0.000 claims description 6
- 238000010298 pulverizing process Methods 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 239000008280 blood Substances 0.000 claims description 4
- 210000004369 blood Anatomy 0.000 claims description 4
- 239000002207 metabolite Substances 0.000 claims description 4
- 244000005700 microbiome Species 0.000 claims description 4
- 239000007921 spray Substances 0.000 claims description 4
- 230000003442 weekly effect Effects 0.000 claims description 4
- 239000000645 desinfectant Substances 0.000 claims description 3
- 238000001514 detection method Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 2
- 238000001816 cooling Methods 0.000 abstract description 12
- 230000000694 effects Effects 0.000 abstract description 9
- 238000002955 isolation Methods 0.000 abstract description 8
- 239000002351 wastewater Substances 0.000 abstract description 8
- 230000008901 benefit Effects 0.000 abstract description 6
- 230000004083 survival effect Effects 0.000 abstract description 5
- 238000010923 batch production Methods 0.000 abstract description 4
- 230000001351 cycling effect Effects 0.000 abstract description 4
- 239000002912 waste gas Substances 0.000 abstract description 3
- 239000007789 gas Substances 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000004698 Polyethylene Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 241000589220 Acetobacter Species 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
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- 230000000249 desinfective effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
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- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
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Abstract
The invention discloses a kind of enzyme microb quantization production system and method, production system includes primary increment fermentor, and the discharge port of primary increment fermentor is communicated with secondary increment fermentor, and the discharge port of secondary increment fermentor is communicated with storage tank after fermentation increment；The present invention is using increment fermentation method step by step, compared with the fermentation method of the prior art, has obvious population advantage, high survival rate substantially reduces fermentation time, virtually reduces production cost, and stable product quality is good between batch production, and the quantization suitable for mass production is fermented；Simultaneously, due to using totally-enclosed disinfection and isolation, cooling method, it is sterilized compared with cooling method with being passed directly into tank body in the prior art, with good isolation effect, guarantee to be passed through in tank body and is unfavorable for other populations that fermenting microbe survives, and the present invention carries out centralized collection to the waste water generated after disinfection, cooling, can continue cycling through utilization, will not generate waste water and gas.
Description
Technical field
The present invention relates to ferment production technical field more particularly to a kind of enzyme microb quantization production systems and method.
Background technique
Ferment is commonly called as " enzyme ", is a kind of protein with catalytic activity, is the catalyst of various biochemical reactions, nothing
By plant or animal, the activity of all life body requires the participation of various enzymes, it is that life entity carries out urging for metabolism
Agent and power producer.Ferment drink currently on the market is mostly based on fruits and vegetables, edible wild herbs, Chinese herbal medicine, edible medicinal mushroom etc.
Raw material is wanted, is fermented through multiple-microorganisms such as Bacillus acidi lactici, saccharomycete, acetobacters.The production process of prior art ferment
In, Concentrated fermentation is carried out by a large amount of raw material, fermentation period is long, and such fermentation method input amount is big, and output is small, population
Not dominant, survival rate is low to can not achieve quantitative inoculation, so that unstable product quality between batch.Further, since input amount
It is larger, therefore a large amount of waste can be generated, such as waste water etc. pollutes the environment, and higher cost, therefore existing skill
The ferment production of art is only applicable to small lot production.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of quantizations of the enzyme microb of stepwise quantization fermentation method to give birth to
System and method is produced, there is obvious population advantage, high survival rate substantially reduces fermentation time, and product between batch production
Quality is stablized, and production cost is reduced, and has the function of wastewater collection, reduces pollution.
In order to solve the above technical problems, the technical scheme is that：Enzyme microb quantifies production system, including at least
One primary increment fermentor, the discharge port of the primary increment fermentor are communicated with secondary increment fermentor, the secondary increasing
The discharge port of amount fermentor is communicated with storage tank after at least one fermentation increment；The primary increment fermentor and the secondary increment
Culture medium agitator tank is communicated at the feed inlet of fermentor；The top of the primary increment fermentor and the secondary increment fermentor
Portion is communicated with water system, steam sterilization system and air supply system respectively, and the output end of the water system is also connected to described
Culture medium agitator tank and the steam sterilization system, the output end of the air supply system store up after being also connected to the fermentation increment
The bottom of tank, the primary increment fermentor and the secondary increment fermentor is communicated with vapor-recovery system, and the steam returns
The output end of receipts system is also connected to the culture medium agitator tank.
The bottom of the primary increment fermentor is also communicated with pulvis production system as a preferred technical solution,.
The pulvis production system includes the pulvis being connected to the primary increment fermentor as a preferred technical solution,
Agitator tank is connected with feed riser machine at the top of the pulvis agitator tank, and the bottom of the pulvis agitator tank is connected with discharging and mentions
Rise machine, it is described discharging elevator terminal at be connected with pulverization and packing machine, be provided with blender in the pulvis agitator tank, it is described
The periphery of pulvis agitator tank is coated with interlayer set, the input terminal and the steam sterilization system of the interlayer set and described for water system
The output end of system connection, the interlayer set is connected to the vapor-recovery system.
The primary increment fermentor is arranged there are two and is connected in parallel as a preferred technical solution, the primary increasing
Amount fermentor is 0.05-0.1 tons of fermentors, and the secondary increment fermentor is 0.5-1 tons of fermentors, is stored up after the fermentation increment
Tank is 3-10 tons of storage tanks.
It is respectively arranged in the primary increment fermentor and the secondary increment fermentor as a preferred technical solution,
Sample-adding dress is respectively arranged on the top tank skin of blender, the primary increment fermentor and the secondary increment fermentor
It sets, respirator and temperature sensor；It is provided on the outer wall of the primary increment fermentor and the secondary increment fermentor
Heat preservation sandwich layer is provided with coil pipe, the input terminal of the coil pipe and the steam sterilization system and the confession in the heat preservation sandwich layer
Water system connection, the output end of the coil pipe are connect with the vapor-recovery system；The primary increment fermentor, the secondary
Air supply pipe is respectively arranged with after increment fermentor and the fermentation increment in storage tank, the input terminal of the air supply pipe is located at outside tank body
It is connected to the air supply system, the output end of the air supply pipe extends to tank bottom；Stirring is provided in the culture medium agitator tank
Device is provided with respirator on the top tank skin of the culture medium agitator tank.
The discharge outlet of the culture medium agitator tank and the secondary increment fermentor is all provided with as a preferred technical solution,
It is equipped with water pump；The water system includes supply equipment, is provided with water-purifying filter at the supply equipment；The air supply system
Including pump, pneumatic filter is correspondingly arranged at the pump；The steam sterilization system includes steam generator；Institute
Stating vapor-recovery system includes header tank.
The production method of enzyme microb quantization, biologic ferment are divided into liquid ferment and powder as a preferred technical solution,
Agent ferment, the production method of microbial liquid ferment quantization, includes the following steps：
The primary culture of step 1 strain：In the lab with specific culture medium will need the microbial strains bred into
Row culture, reaches the requirement of production；
Step 2 primary increment fermentation tank culture：The culture medium raw material for cultivating enzyme microb is put into training as requested
It supports in base agitator tank, and is dissolved by water system, the pH value of adjustment solution reaches desired numerical value after the completion of dissolution, so
Dissolved culture medium is put into primary increment fermentor afterwards, and by water system constant volume to 0.05-0.1 tons, it is other
Primary increment fermentor repeats above operation；Steam sterilization system is opened to first after the completion of all primary increment fermentor ingredients
Material in grade increment fermentor carries out heating sterilization, and material is warming up to 90 DEG C or more stopping heating, keeps the temperature 20-40min, then
Cooled down by water system using cold water, when temperature of charge is reduced to 35-40 DEG C, at the top of primary increment fermentor
Sample adding device pours into the strain of laboratory cultures, sets primary increment fermentation jar temperature after the completion at 30-35 DEG C, cultivates 24-
36h, ventilatory frequency are 5min/8 hours, and stirring frequency is 5min/4 hours, detect colony counts to material；
Step 3 secondary increment fermentation tank culture：The culture medium raw material for cultivating enzyme microb is put into training as requested
It supports in base agitator tank, and is dissolved by water system, the pH value of adjustment solution reaches the numerical value of requirement after the completion of dissolution, so
Dissolved culture medium is put into secondary increment fermentor afterwards, and by water system constant volume to 0.5-1 tons, second order fermentation
Steam sterilization system being opened after the completion of tank ingredient, heating sterilization being carried out to the material in secondary increment fermentor, material is warming up to 90
DEG C or more stop heating, keep the temperature 20-40min, then cooled down by water system with cold water, when temperature of charge is reduced to
35-40 DEG C, qualified primary fermentation material will be cultivated by the discharge port of primary increment fermentor and be put into secondary increment fermentation
In tank, secondary increment fermentation jar temperature is set after the completion at 30-35 DEG C, cultivates 24-36h, and ventilatory frequency is 5min/8 hours, is stirred
Mixing frequency is 10min/4 hours, detects colony counts to material；
Storage tank post-fermentation after step 4 fermentation increment：Qualified secondary will be cultivated by the discharge port of secondary increment fermentor
Fermentation materials are put into after fermentation increment carry out in storage tank after store up fermentation, fermentation time about 50-80 days, wherein ventilatory frequency be
20min/8 hours, the case where every weekly check material pH value, total plate count and metabolite.
The production method of micro organism powder ferment quantization as a preferred technical solution, includes the following steps：
The primary culture of step 1 strain：In the lab with specific culture medium will need the microbial strains bred into
Row culture, reaches the requirement of production；
Step 2 primary increment fermentation tank culture：The culture medium raw material for cultivating enzyme microb is put into training as requested
It supports in base agitator tank, and is dissolved by water system, the pH value of adjustment solution reaches desired numerical value after the completion of dissolution, so
Dissolved culture medium is put into primary increment fermentor afterwards, and by water system constant volume to 0.05-0.1 tons, it is other
Primary increment fermentor repeats above operation；Steam sterilization system is opened to first after the completion of all primary increment fermentor ingredients
Material in grade increment fermentor carries out heating sterilization, and material is warming up to 90 DEG C or more stopping heating, keeps the temperature 20-40min, then
Cooled down by water system using cold water, when temperature of charge is reduced to 35-40 DEG C, at the top of primary increment fermentor
Sample adding device pours into the strain of laboratory cultures, sets primary increment fermentation jar temperature after the completion at 30-35 DEG C, cultivates 24-
36h, ventilatory frequency are 5min/8 hours, and stirring frequency is 5min/4 hours, detect colony counts to material；
Step 3 pulvis agitator tank culture：Heating disinfection is carried out to pulvis agitator tank, 90 DEG C of temperature value or more, continues 10-
Then 20min is put into the pulvis culture medium material mixed in pulvis agitator tank by feed riser machine, open stirring and add
Heat sterilizes to pulvis culture medium material, 85 DEG C of temperature or more, continues 20-40min, is cooled down after the completion by cold water,
Temperature is reduced to 40-45 DEG C, is then put by the discharge port of primary increment fermentor by qualified primary fermentation material is cultivated
Into pulvis agitator tank, pulvis agitator tank temperature is set after the completion at 30-35 DEG C, is cultivated 10-20 days, stirring frequency 10min/
4 hours, colony counts are detected to material；
Step 4 passes through discharging elevator after fermentation and is promoted in pulverization and packing machine.
Material detection colony counts are measured using blood plate observation counting method as a preferred technical solution,.
It needs to carry out spray disinfectant using alcohol when adding materials by sample adding device as a preferred technical solution,.
By adopting the above-described technical solution, the beneficial effects of the invention are as follows：The present invention is using primary increment fermentor, secondary
The increment fermentation method step by step of storage tank after grade increment fermentor, fermentation increment, compared with the fermentation method of the prior art, the present invention
Fermentation process there is obvious population advantage, high survival rate substantially reduces fermentation time, virtually reduces production cost,
And stable product quality is good between batch production, and the quantization suitable for mass production is fermented；Simultaneously as using full envelope
Close disinfection and isolation, cooling method, with being passed directly into tank body disinfection in the prior art compared with cooling method, have it is good every
From effect, guarantee to be passed through in tank body and be unfavorable for other populations that fermenting microbe survives, and to being generated after disinfection, cooling
Waste water carries out centralized collection, can continue cycling through utilization, will not generate waste water and gas.
Detailed description of the invention
The following drawings are only intended to schematically illustrate and explain the present invention, not delimit the scope of the invention.Wherein：
Fig. 1 is the flow sheet of the embodiment of the present invention；
In figure：1- primary increment fermentor；2- grade increment fermentor；Storage tank after 3- fermentation increment；The stirring of 4- culture medium
Tank；5- pulvis agitator tank；6- feed riser machine；7- discharging elevator.
Specific embodiment
With reference to the accompanying drawings and examples, the present invention is further explained.In the following detailed description, only pass through explanation
Mode describes certain exemplary embodiments of the invention.Undoubtedly, those skilled in the art will recognize,
In the case where without departing from the spirit and scope of the present invention, described embodiment can be repaired with a variety of different modes
Just.Therefore, attached drawing and description are regarded as illustrative in nature, and are not intended to limit the scope of the claims.
As shown in Figure 1, enzyme microb quantifies production system, including at least one primary increment fermentor 1, the primary
The discharge port of increment fermentor 1 is communicated with secondary increment fermentor 2, the discharge port of the secondary increment fermentor 2 be communicated with to
Storage tank 3 after a few fermentation increment, the primary increment fermentor 1 are arranged there are two and are connected in parallel, the primary increment hair
Fermentation tank 1 is 0.05-0.1 tons of fermentors, and the secondary increment fermentor 2 is 0.5-1 tons of fermentors, storage tank after the fermentation increment
3 be 3-10 tons of storage tanks, 3 structure of storage tank after the primary increment fermentor 1, the secondary increment fermentor 2, the fermentation increment
At fermenting step by step；Culture medium is communicated at the feed inlet of the primary increment fermentor 1 and the secondary increment fermentor 2 to stir
Tank 4 is mixed, the culture medium raw material that 4 pairs of culture medium agitator tank culture enzyme microbs use dissolves；The primary increment
The top of fermentor 1 and the secondary increment fermentor 2 is communicated with water system, steam sterilization system and air supply system respectively,
The output end of the water system is also connected to the culture medium agitator tank 4 and the steam sterilization system, the air supply system
Output end be also connected to storage tank 3 after the fermentation increment, the primary increment fermentor 1 and the secondary increment fermentor 2
Bottom be communicated with vapor-recovery system, the output end of the vapor-recovery system is also connected to the culture medium agitator tank 4.
The present embodiment further includes control system, the primary increment fermentor 1, the secondary increment fermentor 2, the hair
Storage tank 3, the culture medium agitator tank 4, the water system, steam sterilization system, air supply system, Steam Recovery system after ferment increment
System and pulvis production system are electrically connected to the control system, realize intelligent control by the control system.
Blender, the blender are respectively arranged in the primary increment fermentor 1 and the secondary increment fermentor 2
Top stretch out tank body and be externally connected with stirring motor, for driving blender work to stir the fermentation materials in tank
Mix, be respectively arranged on the top tank skin of the primary increment fermentor 1 and the secondary increment fermentor 2 sample adding device,
Respirator and temperature sensor；Guarantor is provided on the outer wall of the primary increment fermentor 1 and the secondary increment fermentor 2
Warm interlayer, the heat preservation sandwich layer can guarantee there is constant temperature effect in tank, be provided with coil pipe (not in figure in the heat preservation sandwich layer
Show), the input terminal of the coil pipe is connect with the steam sterilization system and the water system, the output end of the coil pipe with
Vapor-recovery system connection, the setting of the coil pipe are and existing so that disinfection, temperature-fall period are to be realized by isolation method
Have to be passed directly into tank body in technology and compare, there is good isolation effect, will not be passed through in guarantee tank body and be unfavorable for zymophyte
Other populations kind survived, and when sterilizing with cooling, the steam sterilization system can provide high-temperature steam and described for water system
System can provide cold water and realize the condensed water and the water system for sterilizing and generating after cooling by disinfection into the excessively described coil pipe
The cold water being passed through can be fed directly in the vapor-recovery system, can continue cycling through utilization, environmental protection and be not in useless
Water exhaust gas；It is respectively set in storage tank 3 after the primary increment fermentor 1, the secondary increment fermentor 2 and the fermentation increment
Have air supply pipe (being not shown), the input terminal of the air supply pipe is located at outside tank body to be connected to the air supply system, the confession
The output end of tracheae extends to tank bottom, for guaranteeing that the fermentation materials positioned at tank bottom can be with gas reaction；The culture medium stirs
It mixes and is provided with blender in tank 4, the top of blender stretches out tank body and is externally connected with stirring motor, for stirring to the culture medium
Culture medium in tank 4 carries out dissolution stirring, is provided with respirator on the top tank skin of the culture medium agitator tank 4.
The sample adding device, the respirator and the temperature sensor are known to ordinary skill in the art
The prior art, in the present embodiment, the effect of the sample adding device are that the strain of laboratory cultures is passed through the sample adding device
It is added in the primary increment fermentor 1；The effect of the respirator is in compensator body and external pressure, and has isolation
Filter device of foreign matter, such as bacterium, impurity etc. guarantee the aseptic for entering inner air tube；The effect of the temperature sensor
It is to be detected to temperature in tank, helps to improve fermentation efficiency.
The discharge outlet of the primary increment fermentor 1 and the secondary increment fermentor 2 is provided with baiting valve and takes
Sample valve, described in material in the primary increment fermentor 1 after fermentation can be discharged into from discharge port by baiting valve
In secondary increment fermentor 2, the material in the secondary increment fermentor 2 can be discharged into institute from discharge port by baiting valve
It states after fermenting increment in storage tank 3, when needs are to the primary increment fermentor 1 and the material in the secondary increment fermentor 2
When detecting bacterium colony, it is sampled observation counting after releasing appropriate material by the sampling valve, sees whether that fermentation is completed；It is described
The discharge outlet of culture medium agitator tank 4 and the secondary increment fermentor 2 is provided with water pump, when the culture medium agitator tank 4
After the completion of interior culture medium dissolution, using water pump by culture medium dissolve put into the primary increment fermentor 1 with it is described
In secondary increment fermentor 2.
The water system includes supply equipment, and water-purifying filter is provided at the supply equipment, in the present embodiment,
R0 wetting system is selected, guarantees that the water being passed into the culture medium agitator tank 4 is free of contamination water, good fermentation ring is provided
Border, in addition, generating cold water after the completion of steam sterilization system disinfection by the supply equipment and entering in the coil pipe
Cool down, the supply equipment and the primary increment fermentor 1, the secondary increment fermentor 2 and the moise-heat sterilization
Water supply valve is provided on the pipeline being connected between system, the control system controls the water supply valve and realizes that intelligence supplies
Water.
The air supply system includes pump, to provide gas in tank, is correspondingly arranged on gas filtration at the pump
Device, for guaranteeing to be passed into storage tank 3 after the secondary increment fermentor 2, the secondary increment fermentor 2, the fermentation increment
Interior gas is pure uncontaminated gases, can effectively reduce the bacterial number for entering in tank body and being unfavorable for fermentation, provide good
Good yeasting, the pump and the primary increment fermentor 1, the secondary increment fermentor 2 and the fermentation increase
Air-supplying valve is provided on the pipeline being connected between storage tank 3 after amount, the control system controls the air-supplying valve and realizes intelligence
It can gas supply.
The steam sterilization system includes steam generator, and the effect of the steam generator is sent out the primary increment
Fermentation tank 1, the secondary increment fermentor 2 sterilize, and carry out disinfection to the material in tank, and carry out disinfection to all pipelines, institute
It states steam generator generation high-temperature steam to carry out disinfection, will form condensed water after the completion of disinfection and proceed to the Steam Recovery system
It is utilized in system by centralized collection, pressure gauge is provided at the steam generator, for measuring and indicating steam generator internal pressure
The size of power, the pipe being connected between the steam generator and the primary increment fermentor 1 and the secondary increment fermentor 2
Road is provided with steam valve, and the control system controls the steam valve and realizes intelligent disinfecting.
The vapor-recovery system includes header tank, the header tank and the primary increment fermentor 1 and the secondary
The valve that catchments is provided on the pipeline being connected between increment fermentor 2, valve realization of catchmenting described in the control system control
Intelligent control.
The bottom of the primary increment fermentor 1 is also communicated with pulvis production system, the pulvis production system include with
The pulvis agitator tank 5 of 1 connection of primary increment fermentor, the top of the pulvis agitator tank 5 are connected with feed riser machine 6,
The bottom of the pulvis agitator tank 5 is connected with discharging elevator 7, is connected with crushing packing at the terminal of the discharging elevator 7
Machine (is not shown), and blender is provided in the pulvis agitator tank 5, and the blender stretches out the pulvis agitator tank 5
It is externally connected with stirring motor, for being stirred to the material being added in the pulvis agitator tank 5, the pulvis agitator tank 5
Periphery be coated with interlayer set, the input terminal of the interlayer set is connected to the steam sterilization system and the water system, institute
State the steam sterilization system and the water system used in pulvis production system with the primary increment fermentor 1,
Storage tank 3 shares after the secondary increment fermentor 2, the fermentation increment for same system, and the steam sterilization system can be real
Water flowing cooling, the output end and the vapor-recovery system of the interlayer set may be implemented in existing heating disinfection, the water system
The water of connection, generation is recycled by vapor-recovery system, can be recycled.
The production method of enzyme microb quantization, biologic ferment are divided into liquid ferment and pulvis ferment, microbial liquid ferment
The production method of element quantization, includes the following steps：
The primary culture of step 1 strain：In the lab with specific culture medium will need the microbial strains bred into
Row culture, reaches the requirement of production；
Step 2 primary increment fermentation tank culture：The culture medium raw material for cultivating enzyme microb is put into training as requested
It supports in base agitator tank 4, and is dissolved by water system, the pH value of adjustment solution reaches desired numerical value after the completion of dissolution,
Then dissolved culture medium is put into primary increment fermentor 1, and by water system constant volume to 0.05-0.1 tons,
Its primary increment fermentor 1 repeats above operation.Steam sterilization system is opened after the completion of all 1 ingredients of primary increment fermentor
Heating sterilization is carried out to the material in primary increment fermentor 1, material is warming up to 90 DEG C or more stopping heating, keeps the temperature 20-
40min is then cooled down by water system using cold water, when temperature of charge is reduced to 35-40 DEG C, is sent out by primary increment
The sample adding device at 1 top of fermentation tank pours into the strain of laboratory cultures, sets primary 1 temperature of increment fermentor after the completion in 30-35
DEG C, 24-36h is cultivated, ventilatory frequency is 5min/8 hours, and stirring frequency is 5min/4 hours, detects colony counts to material；
Step 3 secondary increment fermentation tank culture：The culture medium raw material for cultivating enzyme microb is put into training as requested
It supports in base agitator tank 4, and is dissolved by water system, the pH value of adjustment solution reaches the numerical value of requirement after the completion of dissolution,
Then dissolved culture medium is put into secondary increment fermentor 2, and by water system constant volume to 0.5-1 tons, second level
Steam sterilization system is opened after the completion of fermentor ingredient, and heating sterilization, material liter are carried out to the material in secondary increment fermentor 2
Temperature to 90 DEG C or more stoppings are heated, and are kept the temperature 20-40min, are then cooled down by water system with cold water, when temperature of charge drops
Down to 35-40 DEG C, secondary increment is put into for qualified primary fermentation material is cultivated by the discharge port of primary increment fermentor 1
In fermentor 2, secondary 2 temperature of increment fermentor is set after the completion at 30-35 DEG C, cultivates 24-36h, ventilatory frequency 5min/8
Hour, stirring frequency is 10min/4 hours, detects colony counts to material；
Storage tank post-fermentation after step 4 fermentation increment：Qualified time will be cultivated by the discharge port of secondary increment fermentor 2
Grade fermentation materials are put into after fermentation increment carry out in storage tank 3 after store up fermentation, fermentation time about 50-80 days, wherein ventilation is frequently
The case where rate is 20min/8 hours, every weekly check material pH value, total plate count and metabolite.
The production method of micro organism powder ferment quantization, includes the following steps：
The primary culture of step 1 strain：In the lab with specific culture medium will need the microbial strains bred into
Row culture, reaches the requirement of production；
Step 2 primary increment fermentation tank culture：The culture medium raw material for cultivating enzyme microb is put into training as requested
It supports in base agitator tank 4, and is dissolved by water system, the pH value of adjustment solution reaches desired numerical value after the completion of dissolution,
Then dissolved culture medium is put into primary increment fermentor 1, and by water system constant volume to 0.05-0.1 tons,
Its primary increment fermentor 1 repeats above operation.Steam sterilization system is opened after the completion of all 1 ingredients of primary increment fermentor
Heating sterilization is carried out to the material in primary increment fermentor 1, material is warming up to 90 DEG C or more stopping heating, keeps the temperature 20-
40min is then cooled down by water system using cold water, when temperature of charge is reduced to 35-40 DEG C, is sent out by primary increment
The sample adding device at 1 top of fermentation tank pours into the strain of laboratory cultures, sets primary 1 temperature of increment fermentor after the completion in 30-35
DEG C, 24-36h is cultivated, ventilatory frequency is 5min/8 hours, and stirring frequency is 5min/4 hours, detects colony counts to material；
Step 3 pulvis agitator tank culture：Heating disinfection is carried out to pulvis agitator tank 5,90 DEG C of temperature value or more, continues 10-
20min, then by the pulvis culture medium material mixed by feed riser machine 6 put into pulvis agitator tank 5 in, open stirring and
Heating, sterilizes to pulvis culture medium material, 85 DEG C of temperature or more, continues 20-40min, is dropped after the completion by cold water
Temperature, temperature are reduced to 40-45 DEG C, then will cultivate qualified primary fermentation material by the discharge port of primary increment fermentor 1
It is put into pulvis agitator tank 5, sets 5 temperature of pulvis agitator tank after the completion at 30-35 DEG C, cultivate 10-20 days, stirring frequency is
10min/4 hours, colony counts are detected to material；
Step 4 passes through discharging elevator 7 after fermentation and is promoted in pulverization and packing machine.
Material detection colony counts are measured using blood plate observation counting method, it is ability that blood plate, which observes counting method,
Technology well known to field technique personnel, details are not described herein, and the purpose to material retrieval colony counts is to observe object in tank
Whether material ferments completely, if fermentation will carry out next step operation completely.
It needs to carry out spray disinfectant using alcohol when adding materials by sample adding device, to progress alcohol spray in sample adding device
After spilling disinfection, after standing a few minutes after the volatilization of most of alcohol, using strain is added in sample adding device tank, can effectively it protect
The alien bacteria that the strain that card enters in tank carries is less.
Specific embodiment：
The primary increment fermentor 1 is two and is connected in parallel that the primary increment fermentor 1 is 0.1 ton of fermentor,
The secondary increment fermentor 2 is 1 ton of fermentor, and storage tank 3 is 5 tons of polyethylene storage tanks, also referred to as PE storage after the fermentation increment
Batch can.
The production method of enzyme microb quantization, biologic ferment are divided into liquid ferment and pulvis ferment, microbial liquid ferment
The production method of element quantization, includes the following steps：
The primary culture (laboratory level) of step 1 strain：It will need to breed with specific culture medium in the lab micro-
Biological bacterial strain 1-2kg is cultivated, and the requirement of production is reached；
Step 2 primary increment fermentation tank culture (0.1 ton of fermentor)：The culture medium raw material of enzyme microb will be cultivated
20kg puts into culture medium agitator tank 4 as requested, and is dissolved by water system, and the pH of solution is adjusted after the completion of dissolution
Value reaches desired numerical value, then puts into dissolved culture medium in primary increment fermentor 1, and fixed by water system
Hold 0.1 ton, other primary increment fermentors 1 repeat above operation.It is opened after the completion of all 1 ingredients of primary increment fermentor
Steam sterilization system carries out heating sterilization to the material in primary increment fermentor 1, and material is warming up to 90 DEG C or more stopping heating,
20-40min is kept the temperature, is then cooled down by water system using cold water, when temperature of charge is reduced to 35-40 DEG C, by first
The sample adding device at 1 top of grade increment fermentor pours into the strain of laboratory cultures, sets primary 1 temperature of increment fermentor after the completion
At 30-35 DEG C, 24-36h is cultivated, ventilatory frequency is 5min/8 hours, and stirring frequency is 5min/4 hours, detects bacterium colony to material
Quantity.
Step 3 secondary increment fermentation tank culture：The culture medium raw material 200kg of enzyme microb will be cultivated as requested
It puts into culture medium agitator tank 4, and is dissolved by water system, the pH value of adjustment solution reaches requirement after the completion of dissolution
Then numerical value puts into dissolved culture medium in secondary increment fermentor 2, and by water system constant volume to 1 ton, second level
Steam sterilization system is opened after the completion of fermentor ingredient, and heating sterilization, material liter are carried out to the material in secondary increment fermentor 2
Temperature to 90 DEG C or more stoppings are heated, and are kept the temperature 20-40min, are then cooled down by water system with cold water, when temperature of charge drops
Down to 35-40 DEG C, secondary increment is put into for qualified primary fermentation material is cultivated by the discharge port of primary increment fermentor 1
In fermentor 2, secondary 2 temperature of increment fermentor is set after the completion at 30-35 DEG C, cultivates 24-36h, ventilatory frequency 5min/8
Hour, stirring frequency is 10min/4 hours, detects colony counts to material.
Storage tank post-fermentation after step 4 fermentation increment：Qualified time will be cultivated by the discharge port of secondary increment fermentor 2
Grade fermentation materials are put into after fermentation increment carry out in storage tank 3 after store up fermentation, fermentation time about 50-80 days, wherein ventilation is frequently
The case where rate is 20min/8 hours, every weekly check material pH value, total plate count and metabolite.
The production method of micro organism powder ferment quantization, includes the following steps：
The primary culture (laboratory level) of step 1 strain：It will need to breed with specific culture medium in the lab micro-
Biological bacterial strain 1-2kg is cultivated, and the requirement of production is reached；
Step 2 primary increment fermentation tank culture (0.1 ton of fermentor)：The culture medium raw material of enzyme microb will be cultivated
20kg puts into culture medium agitator tank 4 as requested, and is dissolved by water system, and the pH of solution is adjusted after the completion of dissolution
Value reaches desired numerical value, then puts into dissolved culture medium in primary increment fermentor 1, and fixed by water system
Hold 0.1 ton, other primary increment fermentors 1 repeat above operation.It is opened after the completion of all 1 ingredients of primary increment fermentor
Steam sterilization system carries out heating sterilization to the material in primary increment fermentor 1, and material is warming up to 90 DEG C or more stopping heating,
20-40min is kept the temperature, is then cooled down by water system using cold water, when temperature of charge is reduced to 35-40 DEG C, by first
The sample adding device at 1 top of grade increment fermentor pours into the strain of laboratory cultures, sets primary 1 temperature of increment fermentor after the completion
At 30-35 DEG C, 24-36h is cultivated, ventilatory frequency is 5min/8 hours, and stirring frequency is 5min/4 hours, detects bacterium colony to material
Quantity.
Step 3 pulvis agitator tank culture：Heating disinfection is carried out to pulvis agitator tank 5,90 DEG C of temperature value or more, continues 10-
20min, then by the pulvis culture medium material mixed by feed riser machine 6 put into pulvis agitator tank 5 in, open stirring and
Heating, sterilizes to pulvis culture medium material, 85 DEG C of temperature or more, continues 20-40min, is dropped after the completion by cold water
Temperature, temperature are reduced to 40-45 DEG C, then will cultivate qualified primary fermentation material by the discharge port of primary increment fermentor 1
It is put into pulvis agitator tank 5, sets 5 temperature of pulvis agitator tank after the completion at 30-35 DEG C, cultivate 10-20 days, stirring frequency is
10min/4 hours, colony counts are detected to material.
Step 4 passes through discharging elevator 7 after fermentation and is promoted in pulverization and packing machine.
Certainly, the producer can be according to the practical primary increment fermentor 1 for selecting different size, such as selection two is simultaneously
0.05 ton of fermentor of connection, while putting into the culture medium raw material after the completion of the dissolution in primary increment fermentor 1 and needing to correspond to and determining
Hold 0.05 ton；And 2 0.5 ton of fermentor of corresponding selection of secondary increment fermentor, keep secondary increment fermentation materials total amount
It is 5 times or so multiple proportions of primary increment fermentation materials total amount, while puts into after the completion of the dissolution in secondary increment fermentor 2
Culture medium raw material need corresponding constant volume to 0.5 ton；Storage tank 3 can be selected as needed after the fermentation increment, can be 3 tons of storages
Perhaps the quantity of storage tank 3 may be 2,3 or more to batch can after 10 tons of storage tanks and the fermentation increment.
The present invention is sent out using the gradually increment of storage tank 3 after primary increment fermentor 1, secondary increment fermentor 2, fermentation increment
Ferment mode, compared with the fermentation method of the prior art, fermentation process of the invention have obvious population advantage, high survival rate, greatly
Fermentation time is shortened greatly, virtually reduces production cost, and stable product quality is good between batch production, is suitable for
The quantization of mass production is fermented；It is and directly logical in the prior art simultaneously as using totally-enclosed disinfection and isolation, cooling method
Enter disinfection in tank body has good isolation effect, will not be passed through in guarantee tank body and be unfavorable for zymophyte compared with cooling method
Other populations that kind survives, and the present invention carries out centralized collection to the waste water generated after disinfection, cooling, can continue cycling through benefit
With waste water and gas will not be generated.
The above shows and describes the basic principle, main features and advantages of the invention.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (10)
1. enzyme microb quantifies production system, it is characterised in that：Including at least one primary increment fermentor, the primary increasing
The discharge port of amount fermentor is communicated with secondary increment fermentor, and the discharge port of the secondary increment fermentor is communicated at least one
Storage tank after fermentation increment；Culture medium is communicated at the feed inlet of the primary increment fermentor and the secondary increment fermentor to stir
Mix tank；The top of the primary increment fermentor and the secondary increment fermentor is communicated with water system, moise-heat sterilization respectively
System and air supply system, the output end of the water system are also connected to the culture medium agitator tank and the moise-heat sterilization system
System, the output end of the air supply system are also connected to storage tank after the fermentation increment, the primary increment fermentor and described secondary
The bottom of grade increment fermentor is communicated with vapor-recovery system, and the output end of the vapor-recovery system is also connected to the culture
Base agitator tank.
2. enzyme microb as described in claim 1 quantifies production system, it is characterised in that：The primary increment fermentor
Bottom is also communicated with pulvis production system.
3. enzyme microb as claimed in claim 2 quantifies production system, it is characterised in that：The pulvis production system includes
The pulvis agitator tank being connected to the primary increment fermentor is connected with feed riser machine, institute at the top of the pulvis agitator tank
The bottom for stating pulvis agitator tank is connected with discharging elevator, is connected with pulverization and packing machine at the terminal of the discharging elevator, institute
It states and is provided with blender in pulvis agitator tank, the periphery of the pulvis agitator tank is coated with interlayer set, the input of the interlayer set
End is connected to the steam sterilization system and the water system, and the output end and the vapor-recovery system of the interlayer set connect
It is logical.
4. enzyme microb as described in claim 1 quantifies production system, it is characterised in that：The primary increment fermentor is set
It sets there are two and is connected in parallel, the primary increment fermentor is 0.05-0.1 tons of fermentors, and the secondary increment fermentor is
0.5-1 tons of fermentors, storage tank is 3-10 tons of storage tanks after the fermentation increment.
5. enzyme microb as described in claim 1 quantifies production system, it is characterised in that：It is described primary increment fermentor and
It is respectively arranged with blender in the secondary increment fermentor, the primary increment fermentor and the secondary increment fermentor
Sample adding device, respirator and temperature sensor are respectively arranged on the tank skin of top；The primary increment fermentor and described time
It is provided with heat preservation sandwich layer on the outer wall of grade increment fermentor, coil pipe, the input of the coil pipe are provided in the heat preservation sandwich layer
End is connect with the steam sterilization system and the water system, and the output end of the coil pipe and the vapor-recovery system connect
It connects；Gas supply is respectively arranged in storage tank after the primary increment fermentor, the secondary increment fermentor and the fermentation increment
Pipe, the input terminal of the air supply pipe is located at outside tank body to be connected to the air supply system, and the output end of the air supply pipe extends to tank
Bottom；It is provided with blender in the culture medium agitator tank, is provided with respirator on the top tank skin of the culture medium agitator tank.
6. enzyme microb as described in claim 1 quantifies production system, it is characterised in that：The culture medium agitator tank and institute
The discharge outlet for stating secondary increment fermentor is provided with water pump；The water system includes supply equipment, the supply equipment
Place is provided with water-purifying filter；The air supply system includes pump, is correspondingly arranged on pneumatic filter at the pump；Institute
Stating steam sterilization system includes steam generator；The vapor-recovery system includes header tank.
7. the production method of enzyme microb quantization, which is characterized in that using the described in any item production systems of claim 2 to 6
System, enzyme microb are divided into liquid ferment and pulvis ferment, the production method of microbial liquid ferment quantization, including following step
Suddenly：
The primary culture of step 1 strain：The microbial strains bred will be needed to train with specific culture medium in the lab
It supports, reaches the requirement of production；
Step 2 primary increment fermentation tank culture：The culture medium raw material for cultivating enzyme microb is put into culture medium as requested
It in agitator tank, and is dissolved by water system, the pH value of adjustment solution reaches desired numerical value after the completion of dissolution, then will
Dissolved culture medium is put into primary increment fermentor, and by water system constant volume to 0.05-0.1 tons, other primary
Increment fermentor repeats above operation；Steam sterilization system is opened after the completion of all primary increment fermentor ingredients to increase primary
Material in amount fermentor carries out heating sterilization, and material is warming up to 90 DEG C or more stopping heating, keeps the temperature 20-40min, then pass through
Water system is cooled down using cold water, when temperature of charge is reduced to 35-40 DEG C, passes through the sample-adding at the top of primary increment fermentor
Device pours into the strain of laboratory cultures, sets primary increment fermentation jar temperature after the completion at 30-35 DEG C, cultivates 24-36h, lead to
Gas frequency is 5min/8 hours, and stirring frequency is 5min/4 hours, detects colony counts to material；
Step 3 secondary increment fermentation tank culture：The culture medium raw material for cultivating enzyme microb is put into culture medium as requested
It in agitator tank, and is dissolved by water system, the pH value of adjustment solution reaches the numerical value of requirement after the completion of dissolution, then will
Dissolved culture medium is put into secondary increment fermentor, and by water system constant volume to 0.5-1 tons, second order fermentation tank is matched
Open steam sterilization system after the completion of material and heating sterilization carried out to the material in secondary increment fermentor, material be warming up to 90 DEG C with
Upper stopping heating, keeps the temperature 20-40min, is then cooled down by water system with cold water, when temperature of charge is reduced to 35-40
DEG C, it is put into secondary increment fermentor by the discharge port of primary increment fermentor by qualified primary fermentation material is cultivated,
Secondary increment fermentation jar temperature is set after the completion at 30-35 DEG C, cultivates 24-36h, and ventilatory frequency is 5min/8 hours, stirring frequency
Rate is 10min/4 hours, detects colony counts to material；
Storage tank post-fermentation after step 4 fermentation increment：Qualified secondary fermentation will be cultivated by the discharge port of secondary increment fermentor
Material is put into after fermentation increment carry out in storage tank after store up fermentation, fermentation time about 50-80 days, wherein ventilatory frequency be
20min/8 hours, the case where every weekly check material pH value, total plate count and metabolite.
8. the production method of enzyme microb quantization as claimed in claim 7, it is characterised in that：The quantization of micro organism powder ferment
Production method, include the following steps：
The primary culture of step 1 strain：The microbial strains bred will be needed to train with specific culture medium in the lab
It supports, reaches the requirement of production；
Step 2 primary increment fermentation tank culture：The culture medium raw material for cultivating enzyme microb is put into culture medium as requested
It in agitator tank, and is dissolved by water system, the pH value of adjustment solution reaches desired numerical value after the completion of dissolution, then will
Dissolved culture medium is put into primary increment fermentor, and by water system constant volume to 0.05-0.1 tons, other primary
Increment fermentor repeats above operation；Steam sterilization system is opened after the completion of all primary increment fermentor ingredients to increase primary
Material in amount fermentor carries out heating sterilization, and material is warming up to 90 DEG C or more stopping heating, keeps the temperature 20-40min, then pass through
Water system is cooled down using cold water, when temperature of charge is reduced to 35-40 DEG C, passes through the sample-adding at the top of primary increment fermentor
Device pours into the strain of laboratory cultures, sets primary increment fermentation jar temperature after the completion at 30-35 DEG C, cultivates 24-36h, lead to
Gas frequency is 5min/8 hours, and stirring frequency is 5min/4 hours, detects colony counts to material；
Step 3 pulvis agitator tank culture：Heating disinfection is carried out to pulvis agitator tank, 90 DEG C of temperature value or more, continues 10-
Then 20min is put into the pulvis culture medium material mixed in pulvis agitator tank by feed riser machine, open stirring and add
Heat sterilizes to pulvis culture medium material, 85 DEG C of temperature or more, continues 20-40min, is cooled down after the completion by cold water,
Temperature is reduced to 40-45 DEG C, is then put by the discharge port of primary increment fermentor by qualified primary fermentation material is cultivated
Into pulvis agitator tank, pulvis agitator tank temperature is set after the completion at 30-35 DEG C, is cultivated 10-20 days, stirring frequency 10min/
4 hours, colony counts are detected to material；
Step 4 passes through discharging elevator after fermentation and is promoted in pulverization and packing machine.
9. the production method of enzyme microb quantization as claimed in claim 7 or 8, it is characterised in that：It is observed using blood plate
Counting method measures material detection colony counts.
10. the production method of enzyme microb quantization as claimed in claim 7 or 8, it is characterised in that：Added by sample adding device
It needs to carry out spray disinfectant using alcohol when adding material.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110438003A (en) * | 2019-08-30 | 2019-11-12 | 英诺维尔智能科技（苏州）有限公司 | A kind of big batch cell products large-scale production system |
| WO2021123248A1 (en) | 2019-12-19 | 2021-06-24 | Universität Für Bodenkultur Wien | Continuous reconstitution of process materials from solids |
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