CN106381267B - A kind of simple and efficient solid fermentation apparatus - Google Patents
A kind of simple and efficient solid fermentation apparatus Download PDFInfo
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- CN106381267B CN106381267B CN201610789997.2A CN201610789997A CN106381267B CN 106381267 B CN106381267 B CN 106381267B CN 201610789997 A CN201610789997 A CN 201610789997A CN 106381267 B CN106381267 B CN 106381267B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/16—Solid state fermenters, e.g. for koji production
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/02—Filters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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Abstract
The invention discloses a kind of simple and efficient solid fermentation apparatus,Including discharging opening,Ventilative retainer turnover panel,Compressed air control valve,Cloth strikes off slurry,One-way exhaust valve,Motor,Footstock,Charging aperture,Outer cylinder body,Inner barrel,Agitating shaft,Temperature-measuring port one,Temperature-measuring port two,Thermal controls apparatus,Hygronom,Ultrasonic steam atomising device,Chlorination equipment,The motor is arranged on the upside of installation for fermenting by footstock,The charging aperture is arranged on installation for fermenting top side,The temperature-measuring port one and temperature-measuring port two are separately positioned on the middle part and bottom of Zymolysis Equipment,The electronic thermal controls apparatus is arranged on the lower section of temperature-measuring port one,The hygronom is arranged on Zymolysis Equipment bottom,The ultrasonic steam atomising device is arranged on below hygronom,The chlorination equipment is arranged on the Zymolysis Equipment bottom side relative with ultrasonic steam atomising device,The compression pure air realizes that porous regulation is ventilated by multiple compressed air control valves,The discharging opening is arranged on the bottom of Zymolysis Equipment.
Description
Technical field
The invention belongs to technical field of biological fermentation, is related to a kind of simple and efficient solid fermentation apparatus.
Background technology
Fermentation industry is that the new technology such as traditional zymotic technology and modern DNA restructuring, cell fusion is combined and grown up
Modern biotechnology, and pass through modern chemistry engineering technology, produce utility or be directly used in one kind of industrialized production
Big industrial system, ferment and be divided into according to culture medium state difference：Solid fermentation and liquid fermentation.Fermentation technique is in medicine, food
The application of industry, environmental science, the chemical industry energy, agricultural etc. is more and more, and the technology application of Zymolysis Equipment is also more next therewith
It is more extensive.The equipment that Zymolysis Equipment refers to microbe production purpose product, it must possess suitable microorganism growth and shape
Into the various conditions of product, so as to promote bacteria metabolism, it is allowed to obtain larger yield.
Solid fermentation method be by the use of the agronomic crop fibre such as wheat bran, rice bran, rice husk crude granule as carbon source and the energy, with
The process of production is bred in the solid state substrate top fermentation without free water of certain temperature and humidity.Compared with liquid state fermentation, solid-state
Fermentation has the following advantages that：Wastewater treatment is not needed after reaction, the pollution to environment is small；Do not need dissolved oxygen energy resource consumption low,
Equipment is simple, small investment；Raw material sources are wide, are mostly natural substrates or waste residue, cheap；Fermentation time is short；Fermentation process
Only need gravity-flow ventilation on the premise of controlling temperature and humidity, energy consumption is low.But equally there is also hair for solid fermentation
The problems such as ferment occupation area of equipment is big, and labor intensity is big, and mass-and heat-transfer is difficult, and fermentation parameter hardly possible controls, so in order to guarantee
The advantages of playing solid fermentation to greatest extent, overcomes the shortcomings that existing, good Zymolysis Equipment is particularly critical, how to develop one kind
Convenient use, it is exactly the problem of present invention will study that the solid fermentation apparatus of fermentation efficiency can be improved by, which having,.
The content of the invention
It is an object of the invention to provide a kind of simple and efficient solid fermentation apparatus, is used for biofermentation.
To achieve the above object, a kind of simple and efficient solid fermentation apparatus, including discharging opening, ventilative retainer turnover panel, compression
Air shut-off valve, cloth strike off slurry, one-way exhaust valve, motor, footstock, charging aperture, outer cylinder body, inner barrel, agitating shaft, temperature-measuring port
First, temperature-measuring port two, thermal controls apparatus, hygronom, ultrasonic steam atomising device, chlorination equipment, the motor are arranged on by footstock
On the upside of installation for fermenting, the charging aperture is arranged on installation for fermenting top side, and the temperature-measuring port one and temperature-measuring port two are set respectively
In the middle part of Zymolysis Equipment and bottom, the electronic thermal controls apparatus is arranged on the lower section of temperature-measuring port one, and the hygronom is arranged on hair
Ferment equipment bottom, the ultrasonic steam atomising device are arranged on below hygronom, and the chlorination equipment is arranged under Zymolysis Equipment
The portion side relative with ultrasonic steam atomising device, the compression pure air are realized by multiple compressed air control valves
Porous regulation ventilation, the discharging opening are arranged on the bottom of Zymolysis Equipment.
Preferably, the ultrasonic steam atomising device communicates with the interlayer of outer cylinder body and inner barrel, and the inner barrel is equal
Even porous breathable structure, atomizing steam can be made to enter in bucket, so as to uniformly effectively adjust fermentation humidity.
Preferably, the filter membrane that one layer of aperture is 0.05 μm is covered at the compressed air inlet on inner barrel outer wall, can
Miscellaneous bacteria in effectively catching air, reduce the probability of living contaminants.
Preferably, the thermal controls apparatus realizes the control of temperature, institute by way of the mixing regulation of electronic and solar energy
State thermal controls apparatus and be internally provided with temperature bound sensor, to realize the suitable temperature ranges needed for fermentation.
Preferably, the chlorination equipment Interior Disinfection liquid, realize that cylinder sterilizes by way of electric heating evaporation, sterilization effect
Fruit is good, overcomes sterilization dead angle residue problem.
Preferably, the thimerosal is citric acid and Chinese medicine extract with 1:The thimerosal that 4 mass ratio mixes, institute
The main component and extracting method for stating Chinese medicine extract be：Take Chinese medicine frutus cnidii 50-85 parts, blue gum 42-78 parts, gingko 37-
72 parts, smilax 30-63 parts, honeysuckle 26-56 parts, radix glycyrrhizae 20-48 parts, be dried processing after co-grinding, with 10-12 times
The ethanol solution refluxing extraction that the mass concentration of amount is 70% 3 times, each extraction time is 30min, and ethanol is adopted while extraction
With microwave irradiation assisted extraction, the microwave power is 280MPa, and each microwave radiation technology irradiation time is 5min, with milipore filter mistake
Filtrate is obtained after filter, the filtrate is condensed into the solution that water content is 35%, is the Chinese medicine extract after sterilization treatment, will
Citric acid adds deionized water to dilute in one times of addition mixer, then is added thereto Chinese medicine extract in the ratio, with 850r/
Min rotating speed stirring 15min, as described thimerosal, no chemical residues pollution, long-term use do not interfere with fermentation condition.
Preferably, bed of cloth thickness is 200mm in described Fig. 1, and fermentation reaction is uniform under this thickness.
Preferably, the ventilative retainer turnover panel has 7, in Fig. 1 label 1. -7..
The advantage of the invention is that：A kind of simple and efficient solid fermentation apparatus of the present invention, simple in construction, reasonable in design, work(
Can be complete, the optimization design of solid fermentation can be achieved, improve fermented quality and fermentation efficiency.The present invention sets temperature control device, can
To adjust fermentation temperature in suitable range intervals, hygronom and ultrasonic steam atomising device can ensure suitable wet of fermentation process
Degree, self-configuring chlorination equipment, pasteurised completely can be carried out to device, thimerosal is matched using natural traditional Chinese medicine extract, reducing
Influence of the residual to fermentation condition is learned, the harmful substance in membrane filtration air is used at compressed air inlet, is avoided to hair
The influence of ferment condition.
Brief description of the drawings
Fig. 1 is the positive structure schematic of simple and efficient solid fermentation apparatus；
Wherein, 1- discharging openings；The ventilative retainer turnover panels of 2-；3- compressed air control valves；4- clothes strike off slurry；5- is unidirectional
Air bleeding valve；6- motors；7- footstocks；8- charging apertures；9- outer cylinder bodies；10- inner barrels；11- agitating shafts；12- temperature-measuring ports one；13- heat
Control device；14- hygronoms；15- ultrasonic steam atomising devices；16- chlorination equipments；17- temperature-measuring ports two.
Embodiment
Hereinafter, with reference to accompanying drawing 1 and embodiment, the embodiment of the present invention is described in detail：
As Fig. 1 shows a kind of simple and efficient solid fermentation apparatus of the present invention, including discharging opening 1, ventilative retainer turnover panel 2,
Compressed air control valve 3, cloth strike off slurry 4, one-way exhaust valve 5, motor 6, footstock 7, charging aperture 8, outer cylinder body 9, inner barrel 10,
Agitating shaft 11, temperature-measuring port 1, temperature-measuring port 2 17, thermal controls apparatus 13, hygronom 14, ultrasonic steam atomising device 15, sterilization dress
16 are put, the motor 6 is arranged on the upside of installation for fermenting by footstock 7, and the charging aperture 8 is arranged on installation for fermenting top side,
The temperature-measuring port 1 and temperature-measuring port 2 17 are separately positioned on the middle part and bottom of Zymolysis Equipment, and the electronic thermal controls apparatus 13 is set
Put below temperature-measuring port 1, the hygronom 14 is arranged on Zymolysis Equipment bottom, and the ultrasonic steam atomising device 15 is set
Below hygronom 14, the chlorination equipment 16 is arranged on one relative with ultrasonic steam atomising device 15 of Zymolysis Equipment bottom
Side, the compression pure air realize that porous regulation is ventilated by multiple compressed air control valves 3, and the discharging opening 1 is set
Put in the bottom of Zymolysis Equipment；The ultrasonic steam atomising device 15 communicates with the interlayer of outer cylinder body 9 and inner barrel 10, described interior
Cylinder 10 is even porous ventilating structure, and atomizing steam can be made to enter in bucket, so as to uniformly effectively adjust fermentation humidity；It is described
The filter membrane that one layer of aperture is 0.05 μm is covered at compressed air inlet on the outer wall of inner barrel 10, can be in effectively catching air it is miscellaneous
Bacterium, reduce the probability of living contaminants；The thermal controls apparatus 13 realizes temperature by way of the mixing regulation of electronic and solar energy
The control of degree, the thermal controls apparatus 13 are internally provided with temperature bound sensor, to realize the suitable temperature model needed for fermentation
Enclose；The Interior Disinfection liquid of chlorination equipment 16, realize that cylinder sterilizes by way of electric heating evaporation, Disinfection Effect is good, overcomes
Sterilization dead angle residue problem；The thimerosal is citric acid and Chinese medicine extract with 1:The sterilization that 4 mass ratio mixes
Liquid, the main component and extracting method of the Chinese medicine extract are：Take Chinese medicine frutus cnidii 50-85 parts, blue gum 42-78 parts, white
Fruit 37-72 parts, smilax 30-63 parts, honeysuckle 26-56 parts, radix glycyrrhizae 20-48 parts, co-grinding after processing is dried, used
The ethanol solution refluxing extraction that the 10-12 times of mass concentration measured is 70% 3 times, each extraction time are 30min, ethanol extraction
Simultaneously use microwave irradiation assisted extraction, the microwave power is 280MPa, and each microwave radiation technology irradiation time is 5min, with surpass
Filtrate is obtained after membrane filtration, the filtrate is condensed into the solution that water content is 35%, is that the Chinese medicine carries after sterilization treatment
Liquid is taken, citric acid plus deionized water are diluted in one times of addition mixer, then is added thereto Chinese medicine extract in the ratio,
15min, as described thimerosal are stirred with 850r/min rotating speed, no chemical residues pollute, and long-term use does not interfere with fermentation
Condition, no chemical residues pollution, long-term use do not interfere with fermentation condition；Bed of cloth thickness is 200mm in described Fig. 1, and this is thick
The lower fermentation reaction of degree is uniform；The ventilative retainer turnover panel 2 has 7, in Fig. 1 label 1. -7..
The present invention is not limited to above-mentioned embodiment, in the case of without departing substantially from the substantive content of the present invention, this area skill
Art personnel it is conceivable that any deformation, improvement, replacement each fall within the scope of the present invention.
Claims (1)
1. a kind of simple and efficient solid fermentation apparatus, it is characterised in that including discharging opening (1), ventilative retainer turnover panel (2), compression
Air shut-off valve (3), cloth strike off slurry (4), one-way exhaust valve (5), motor (6), footstock (7), charging aperture (8), outer cylinder body
(9), inner barrel (10), agitating shaft (11), temperature-measuring port one (12), temperature-measuring port two (17), thermal controls apparatus (13), hygronom (14),
Ultrasonic steam atomising device (15), chlorination equipment (16), the motor (6) are arranged on the upside of installation for fermenting by footstock (7), institute
State charging aperture (8) and be arranged on installation for fermenting top side, the temperature-measuring port one (12) and temperature-measuring port two (17) are separately positioned on hair
The middle part and bottom of ferment equipment, the thermal controls apparatus (13) are arranged on below temperature-measuring port one (12), and the hygronom (14) is set
In Zymolysis Equipment bottom, the ultrasonic steam atomising device (15) is arranged on below hygronom (14), the chlorination equipment (16)
Zymolysis Equipment bottom and the relative side of ultrasonic steam atomising device (15) are arranged on, the compression pure air passes through multiple pressures
Contracting air shut-off valve (3) realizes porous regulation ventilation, and the discharging opening (1) is arranged on the bottom of Zymolysis Equipment；
The ultrasonic steam atomising device (15) communicates with the interlayer of outer cylinder body (9) and inner barrel (10), the inner barrel (10)
For even porous ventilating structure, atomizing steam can be made to enter in bucket；Inner barrel (10) outer wall overlying at the compressed air inlet
One layer of aperture of lid is 0.05 μm of filter membrane；The thermal controls apparatus (13) is internally provided with temperature bound sensor；It is described to disappear
Malicious device (16) Interior Disinfection liquid；The thimerosal is citric acid and Chinese medicine extract with 1:What 4 mass ratio mixed disappears
Venom, the main component and extracting method of the Chinese medicine extract are：Take Chinese medicine frutus cnidii 50-85 parts, blue gum 42-78 parts,
Gingko 37-72 parts, smilax 30-63 parts, honeysuckle 26-56 parts, radix glycyrrhizae 20-48 parts, co-grinding after processing is dried, used
The ethanol solution refluxing extraction that the 10-12 times of mass concentration measured is 70% 3 times, each extraction time are 30min, ethanol extraction
Simultaneously use microwave irradiation assisted extraction, the microwave power is 280MPa, and each microwave radiation technology irradiation time is 5min, with surpass
Filtrate is obtained after membrane filtration, the filtrate is condensed into the solution that water content is 35%, is that the Chinese medicine carries after sterilization treatment
Take liquid；Citric acid plus deionized water are diluted in one times of addition mixer, then are added thereto Chinese medicine extract in the ratio,
15min, as described thimerosal are stirred with 850r/min rotating speed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610789997.2A CN106381267B (en) | 2016-08-31 | 2016-08-31 | A kind of simple and efficient solid fermentation apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610789997.2A CN106381267B (en) | 2016-08-31 | 2016-08-31 | A kind of simple and efficient solid fermentation apparatus |
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| Publication Number | Publication Date |
|---|---|
| CN106381267A CN106381267A (en) | 2017-02-08 |
| CN106381267B true CN106381267B (en) | 2018-01-09 |
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| CN201610789997.2A Expired - Fee Related CN106381267B (en) | 2016-08-31 | 2016-08-31 | A kind of simple and efficient solid fermentation apparatus |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108102895B (en) * | 2017-12-13 | 2021-06-04 | 广西南宁秀珀生物科技有限公司 | Multilayer solid material fermentation device |
| CN108456012B (en) * | 2018-04-19 | 2023-07-07 | 姚永强 | Ectopic fermentation machine for livestock and poultry waste |
| CN108440043B (en) * | 2018-04-19 | 2023-07-11 | 姚永强 | Tipping bucket device |
| CN113801782B (en) * | 2021-09-23 | 2023-09-01 | 郑州智拓生物科技有限公司 | System and method for realizing rapid culture of microbial agent |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2567225Y (en) * | 2002-08-09 | 2003-08-20 | 中国科学院过程工程研究所 | Device for maitaining high water activity in solid state fermentation process |
| CN201420071Y (en) * | 2009-05-08 | 2010-03-10 | 安徽丰原发酵技术工程研究有限公司 | Solid state fermentation device |
| CN102358883A (en) * | 2011-10-31 | 2012-02-22 | 江苏海华生物科技有限公司 | Rotary shaft type solid state fermentation tank |
| CN102732417B (en) * | 2012-06-15 | 2013-06-19 | 卢行娥 | Emulsifying-type aerobic fermentation tank |
| CN204569936U (en) * | 2015-04-24 | 2015-08-19 | 江南大学 | A kind of small-sized fermentation tank group system |
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