CN101496574B - Closed type solid fermentation reaction device - Google Patents
Closed type solid fermentation reaction device Download PDFInfo
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- CN101496574B CN101496574B CN2009100471297A CN200910047129A CN101496574B CN 101496574 B CN101496574 B CN 101496574B CN 2009100471297 A CN2009100471297 A CN 2009100471297A CN 200910047129 A CN200910047129 A CN 200910047129A CN 101496574 B CN101496574 B CN 101496574B
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- 230000004151 fermentation Effects 0.000 title claims abstract description 45
- 239000007787 solid Substances 0.000 title claims abstract description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 31
- 238000003756 stirring Methods 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims description 16
- 239000012528 membrane Substances 0.000 claims description 14
- 238000010438 heat treatment Methods 0.000 claims description 11
- 238000001816 cooling Methods 0.000 claims description 9
- 239000011343 solid material Substances 0.000 claims description 9
- 239000006185 dispersion Substances 0.000 claims description 6
- 229910001220 stainless steel Inorganic materials 0.000 claims description 6
- 239000010935 stainless steel Substances 0.000 claims description 6
- 239000000945 filler Substances 0.000 claims description 4
- 238000009413 insulation Methods 0.000 claims description 4
- BFKJFAAPBSQJPD-UHFFFAOYSA-N tetrafluoroethene Chemical compound FC(F)=C(F)F BFKJFAAPBSQJPD-UHFFFAOYSA-N 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 3
- 230000003020 moisturizing effect Effects 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 10
- 238000010563 solid-state fermentation Methods 0.000 abstract description 3
- 239000000463 material Substances 0.000 description 7
- 230000001954 sterilising effect Effects 0.000 description 6
- 238000004659 sterilization and disinfection Methods 0.000 description 6
- 238000009423 ventilation Methods 0.000 description 5
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- 238000011081 inoculation Methods 0.000 description 2
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- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
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Abstract
The invention relates to a closed type solid fermentation reactor, which belongs to the field of solid state fermentation reactors and comprises a closed container and an air intake system, wherein the air intake system is connected with the closed container, adds water and introduces moisture-keeping gas into the closed container to perform solid fermentation in the process of fermentation. The air intake system comprises a humidifier and a water storage bottle to introduce moisture-keeping air and moisture into the closed container respectively, and a control system to perform time control on the reactor. The closed container is a cylindrical container of which the bottom is provided with an air chamber. The air chamber is separated from the main part of the container by an air distribution plate; and the inside of the closed container is provided with a stirring device of which the top is provided with a moisture atomizing device. The humidifier can well control the temperature and the humidity of the gas passed into the reactor. The reactor is operated closely and controls the temperature therein in the process of the fermentation through the temperature and the flow of the gas passed therein. With the stirring device, the moisture can be added in the process of the fermentation, the fermentation parameters can be controlled well, and the quality and the yield of the fermentation can be improved.
Description
Technical field
The invention belongs to the solid state fermentation field, particularly a kind of systematization solid-state fermentation reactor.
Background technology
Leaven cultivating equipment plays a part very important in making leaven of soy sauce technique.Making leaven of soy sauce still mainly adopts bamboo plaque mode at present, and this mode mechanization degree is low, and labour intensity is large, and can not reach sterile working, particularly runs into the yellow plum sky, and the sauce song is easy to microbiological contamination.Therefore the soy sauce brewing industry is badly in need of a kind of mechanization intensity height, the equipment of closed-loop operation.Some brewing equipments occurred now, but these equipment manufacturing cost are expensive, are difficult to large-scale promotion.A topmost problem is exactly heat dissipation problem in solid fermentation, because the solid substrate thermal conductance is poor, causes heat convection current and heat transfer efficiency low, on the heat production peak, bed temperature sharply rises, and thermograde occurs, must reduce during the fermentation the appearance of thermograde as far as possible.General the mode of evaporation is controlled temperature by cross-ventilation, but must consider that cross-ventilation to the effect of moisture evaporation, prevents the moisture excessive loss.
Summary of the invention
The object of the present invention is to provide a kind of closed type solid fermentation reaction device, this device is fully airtight, can realize sterile working, guarantees the security of fermented product.
For reaching above purpose, solution of the present invention is:
A kind of solid fermentation reaction device, it comprises closed container, gas handling system, gas handling system connecting sealed container, gas handling system is added moisture and is passed into moisturizing gas to closed container during the fermentation and carries out solid fermentation.
Further, it also comprises control system, and control system is carried out time control to reaction unit.
Described closed container comprises agitating device, atomizer, dispersion plate, temperature electrode, humidity electrode, central shaft, central shaft is connected motor and is connected by belt with the closed container outside, central shaft is provided with agitating device and rotates stirring so that electric machine rotation drives agitating device, atomizer is located at the closed container top, closed container is separated out an air chamber by dispersion plate in its bottom, and air chamber is provided with air inlet.
Described closed container is that the stainless steel cylindricality is simplified, and a side also is provided with the visor of glass.
Described closed container outer wall can also be established insulation water-bath or annular-heating band.
Described temperature electrode is three, lays respectively at the top and measures round top gas phase temperature, solid material temperature and be positioned at air chamber mensuration intake air temperature; The humidity electrode then is positioned at the tank body top and measures gas phase humidity.
Described agitating device is the helical ribbon blade, is welded by two width annulus identical with diameter, and the ribbon width is 1/10 of barrel diameter, is fixed on central shaft by a head screw.
Described gas handling system, comprise air compressor, filter membrane, gas bleeder valve, gas flowmeter, operated pneumatic valve, water-storing bottle and humidifier, air compressor connects gas flowmeter and connects respectively humidifier and water-storing bottle by operated pneumatic valve, the humidifier air inlet that is connected with the air chamber of closed container, water-storing bottle is connected into water with the atomizer of closed container; Described filter membrane is two, and the first filter membrane and the second filter membrane are located at respectively the gas flowmeter both sides, and gas bleeder valve is two also, is located between filter membrane and the gas flowmeter.
Described humidifier top is provided with cover plate, is provided with the thermocouple, air inlet pipe, gas outlet, the temperature electrode that pass cover plate in the humidifier.The air inlet pipe bottom is ring-type, and has aperture downwards; Humidifier is provided with filler and discharge outlet, and the filler is connected with the water-storing bottle that adds water; Humidifier also is provided with the liquid level gauge of measuring the inside liquid level height.
Described humidifier is the cylindrical stainless steel cylindrical shell that certain liquid level is housed, described liquid is water, water is provided by liquid level is poor by water-storing bottle, its water temperature is stabilized in setting value by heating and cooling Double-direction Temperature controller, and humidifier top cover plate seals by flange and by ring-type tetrafluoroethene band.
In the closed container, be connected with upper cover plate at central shaft and adopt mechanical dynamic seal, in needs sealing place the tetrafluoroethene material is arranged.After tank body and material sterilization, use liquid state fermentation pyrosphere method commonly used during inoculation, access solid seed.Adopt the sterilization of humidifier wet-hot steam method for the sterilization of humidifier and pipeline.In the time of need to adding moisture to material, adopt after filtration that the aseptic gas of membrane filtration is pressed into tank body with the sterilized water in the water-storing bottle, and be sprayed on the material, open simultaneously and stir, moisture is mixed.In whole solid material sweat, this device can be realized sterile working, guarantees fermented quality.
Owing to adopted such scheme, the present invention has following characteristics: the present invention is the temperature of controlled fermentation process well, by inlet air control bed temperature, owing to added the distinctive stirring system in the solid environment, can add voluntarily during the fermentation moisture, prevent because the moisture excessive loss that forced convertion ventilation heat radiation causes.This reactor closed-loop operation can be with the temperature of solid fermentation, moisture, and ventilation well is coupled together, and reaches good ferment effect.
Description of drawings
Fig. 1 is the solid fermentation reaction device schematic diagram.
Fig. 2 is solid fermentation reactor air-channel system schematic diagram.
Fig. 3 is the humidifier structural representation.
The specific embodiment
The present invention is further illustrated below in conjunction with the accompanying drawing illustrated embodiment.
This invention solid fermentation reactor comprises closed container, gas handling system.
Belt 1 couples together tank body central shaft 13 and motor 14, adds that electric current passes through speed change, and potentiometer makes speed of agitator be controlled at 0-15rpm after regulating, and satisfies the slow-revving requirement of der Pilz solid fermentation.
Paddle in the closed container is helical ribbon blade 6, and it is to be welded by two width annulus identical with diameter.The ribbon width is 1/10 of barrel diameter, is fixed on the central shaft 13 by a head screw, and when motor 14 rotated, stirrer paddle rotated, and solid material is overturn from the bottom up along blade, and falls from blade centre and tank skin, and material can well be mixed.
Closed container is the stainless steel column-shaped barrel, and a side is provided with the visor 7 of glass.Visor is mainly in order to observe during the fermentation the microbial growth situation, the growth of mycelia and the formation of spore.Closed container makes the bottom form an air chamber 11 by dispersion plate 8.Gas enters behind air chamber 11 interior process dispersion plates 8 from bottom air inlet 10 and enters the fermentation tank main part, and then the gas vent 5 from top flows out.Being equipped with three temperature electrodes in the closed container, is respectively for the gas phase temperature electrode 2 of measuring round top gas phase temperature, measures the solid-state temperature electrode 4 of solid material temperature and the gas phase temperature electrode 9 of the air chamber temperature of measuring air inlet.A humidity electrode 3 is positioned at the tank body top and measures gas phase humidity.The closed container outer wall can add insulation water-bath or annular-heating band, at the fermentation initial stage, can promote spore germination by the heat that insulation water-bath or annular-heating band provide.
The closed container top also has an atomizer 12.When needs added moisture to tank body, the second operated pneumatic valve 20 that leads to one tunnel gas of humidifier 23 was closed, simultaneously, open the first operated pneumatic valve 19 of water injector, gas leads to the first water-storing bottle 22 presses to fermentation tank with the sterilized water in the bottle, by atomizer 12, sprays moisture.Meanwhile open and stir, make mixing of materials even.
In the gas handling system, gas filters by the first filter membrane 16 after being compressed by air compressor 15, regulate flow velocity through mass air flow sensor 18 again, further filter through the second filter membrane 17 again, then through behind humidifier 23 heating (cooling) humidifications, enter closed container bottom air chamber 11.When not needing to add moisture, the second operated pneumatic valve 20 of humidifier one tunnel often is in to be opened the stage.Be in security consideration, be provided with gas bleeder valve at gas flowmeter 18 and the first filter membrane 16, the second filter membrane 17 amongs, in whole sweat, air compressor machine 15 is under the operating state always, when needing to stop to ventilate when sampling or on the technique, at this moment need to open air-outlet valve and lose heart.Humidifier 23 is for being equipped with the cylindrical stainless steel cylindrical shell of certain liquid level.Water temperature in the humidifier 23 is stabilized in setting value by heating and cooling Double-direction Temperature controller.Thermocouple 24 heating are arranged in the humidifier 23, when temperature surpasses setting value, open cooling, colling end is connected with a fan.The gas that enters humidifier 23 enters the humidifier bottom by air inlet pipe 25, and the bottom inflow pipe is ring-type, and has aperture downwards.Gas is flowed out by the gas outlet 26 at top by the liquid level of humidifier certain altitude, has a temperature electrode 27 to be used for measuring the temperature of humidifier internal water in the middle of the humidifier 23.Humidifier 23 top cover plates seal by flange 28 and by ring-type tetrafluoroethene band.Humidifier 23 interior water are to be provided by liquid level is poor by the second water-storing bottle 21, flow to from humidifier water inlet 30.When fermentation ends, humidifier 23 internal water should in time be discharged by discharge outlet 31.Two liquid level gauges 29 can be observed the inside liquid level height about humidifier 23 1 sides were provided with in addition, prevented that water level is excessively low, affects the humidification effect.The humidifier temperature can be controlled with two-way control temperature controller, mainly is for enough temperature and humidities are provided to gas, by water temperature in the regulating, raising humidity apparatus, and when gas flow is crossed humidifier, and hydrothermal exchange in the humidifier.Make air obtain heating or cooling, thereby the temperature of air is controlled.In addition, when air passed through humidifier, the moisture of air was able to saturated, becomes moisturizing gas.Forced Air Convection was to the evaporation of the moisture of solid matrix in the reactor when this was conducive to reduce fermentation, and the increase of bed pressure drop and bed shrink, and prevent the appearance in obvious circulation of air slit, affect growth of microorganism.
Time and cycle that control system control is stirred, the time of water spray and cycle.This realizes by the time relay, generally pre-sets duration and interval that the time relay is opened, controls the running of motor, thereby can control opening time and the interval of stirring.Water spraying time and interval also are like this.
Temperature and the flow-control fermentation tank temperature of the gas of reactor of the present invention by entering closed container.When growth of microorganism is vigorous in the tank body, can produce amount of heat.The heat that removes by the tank skin cooling is limited, therefore considers to evaporate the major way that removes as heat by convection current and the moisture of air.By temperature and the flow-control temperature that enters tank body gas.When but air mass flow was larger, the evaporation of the moisture of solid material is serious, and was very limited by the initial moisture that strengthens solid material.Therefore the present invention adopts in the way method of adding moisture to tank body to add moisture, and has added stirring system and make moisture distribution even.
This device is fully airtight, after the device sterilization, is difficult for microbiological contamination.Reach airtight aseptic purpose.
Example
At first the fermentation solid material is placed in the solid reactor, with reactor and sterilized water water-storing bottle in 121 ℃ of sterilizations of autoclave 30min.Add sterilized water to certain altitude to humidifier.Open the humidifier heater switch, sterilized water is heated to boiling and produces steam, continuous heating 15min makes humidifier and gas pipeline reach sterilization effect.Behind the 15min, close heater switch, the regulating, raising humidity apparatus temperature is to fermentation temperature, and the humidifier colling end is connected with fan, and the humidifier cooling is opened, and opens simultaneously air compressor, makes gas pass through humidifier, accelerates the cooling of humidifier.Treat the humidifier temperature stabilization when fermentation temperature, the humidifier gas outlet be connected with the closed container air inlet that gas enters the solid fermentation reactor.Open the inoculation mouth, under aseptic technique, add the solid seed.Open and stir, seed and solid material are mixed.Behind the 15min, close stirring.Begin fermentation.
In the microbial fermentation stage, show the fermentation situation by the temperature and humidity electrode.Prescribe a time limit when temperature surpasses on the microbial fermentation, strengthen ventilation flow rate, reduce the humidifier temperature, if thalline self allows stirring, can open stirring and reduce temperature.When humidity is reduced to certain value, open water injector.Close a road the gas operated pneumatic valve that leads to humidifier, simultaneously, open the operated pneumatic valve of water injector, gas leads to water-storing bottle presses to tank body with the sterilized water in the bottle, by atomizer, sprays moisture.Meanwhile open agitating device, make mixing of materials even.
The time of stirring and spraying water and cycle can the manual or automatically controls by the time relay.
The above-mentioned description to embodiment is can understand and apply the invention for ease of those skilled in the art.The person skilled in the art obviously can easily make various modifications to these embodiment, and needn't pass through performing creative labour being applied in the General Principle of this explanation among other embodiment.Therefore, the invention is not restricted to the embodiment here, those skilled in the art should be within protection scope of the present invention for improvement and modification that the present invention makes according to announcement of the present invention.
Claims (5)
1. solid fermentation reaction device, it is characterized in that: it comprises closed container, gas handling system, gas handling system connecting sealed container, gas handling system is added moisture and is passed into moisturizing gas to closed container during the fermentation and carries out solid fermentation;
Also comprise control system, control system is carried out time control to reaction unit;
Described closed container comprises agitating device, atomizer, dispersion plate, temperature electrode, humidity electrode, central shaft, central shaft is connected motor and is connected by belt with the closed container outside, central shaft is provided with agitating device and rotates stirring so that electric machine rotation drives agitating device, atomizer is located at the closed container top, closed container is separated out an air chamber by dispersion plate in its bottom, and air chamber is provided with air inlet;
Described gas handling system, comprise air compressor, filter membrane, gas bleeder valve, gas flowmeter, operated pneumatic valve, water-storing bottle and humidifier, air compressor connects gas flowmeter and the operated pneumatic valve by separately connects respectively humidifier and water-storing bottle, the humidifier air inlet that is connected with the air chamber of closed container, water-storing bottle is connected into water with the atomizer of closed container; Described filter membrane is two, and the first filter membrane and the second filter membrane are located at respectively the gas flowmeter both sides, and gas bleeder valve is two also, is located between filter membrane and the gas flowmeter;
Described humidifier top is provided with cover plate, is provided with the thermocouple, air inlet pipe, gas outlet, the temperature electrode that pass cover plate in the humidifier, and the air inlet pipe bottom is ring-type, and has aperture downwards; Humidifier is provided with filler and discharge outlet, and the filler is connected with the water-storing bottle that adds water; Humidifier also is provided with the liquid level gauge of measuring the inside liquid level height;
Described humidifier is the cylindrical stainless steel cylindrical shell that liquid level is housed, described liquid is water, water is provided by liquid level is poor by water-storing bottle, and its water temperature is stabilized in setting value by heating and cooling Double-direction Temperature controller, and humidifier top cover plate seals by flange and by ring-type tetrafluoroethene band.
2. solid fermentation reaction device as claimed in claim 1, it is characterized in that: described closed container is the stainless steel column-shaped barrel, and a side also is provided with the visor of glass.
3. solid fermentation reaction device as claimed in claim 1 is characterized in that: described closed container outer wall also is provided with insulation water-bath or annular-heating band.
4. solid fermentation reaction device as claimed in claim 1, it is characterized in that: described temperature electrode is three, lays respectively at the top and measures round top gas phase temperature, solid material temperature and be positioned at air chamber mensuration intake air temperature; The humidity electrode then is positioned at the tank body top and measures gas phase humidity.
5. solid fermentation reaction device as claimed in claim 1, it is characterized in that: described agitating device is the helical ribbon blade, be welded by two width annulus identical with diameter, the ribbon width is 1/10 of barrel diameter, is fixed on central shaft by a head screw.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100471297A CN101496574B (en) | 2009-03-06 | 2009-03-06 | Closed type solid fermentation reaction device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009100471297A CN101496574B (en) | 2009-03-06 | 2009-03-06 | Closed type solid fermentation reaction device |
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| CN101496574A CN101496574A (en) | 2009-08-05 |
| CN101496574B true CN101496574B (en) | 2013-01-16 |
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| CN2009100471297A Expired - Fee Related CN101496574B (en) | 2009-03-06 | 2009-03-06 | Closed type solid fermentation reaction device |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102220223B (en) * | 2011-03-25 | 2014-08-06 | 重庆良酩科技发展有限公司 | Solid-state fermentation material processing device |
| CN102199521A (en) * | 2011-04-07 | 2011-09-28 | 华东理工大学 | Enclosed solid state fermentation bioreactor |
| CN102499190A (en) * | 2011-11-23 | 2012-06-20 | 广东省昆虫研究所 | Ventilating and stirring type Hermetia illucens bioprocessor |
| AU2013334175B2 (en) * | 2012-10-26 | 2017-09-28 | Massachusetts Institute Of Technology | Humidity control in chemical reactors |
| CN102925336B (en) * | 2012-10-31 | 2014-06-04 | 中国科学院过程工程研究所 | Chained lifting solid fermentation tank and fermentation method |
| CN103695294B (en) * | 2013-12-20 | 2015-08-19 | 中粮生物化学（安徽）股份有限公司 | Microorganism solid-state culture system |
| CN105505771A (en) * | 2016-02-04 | 2016-04-20 | 山东百德生物科技有限公司 | Multifunctional solid-phase saccharification and fermentation equipment |
| CN106190831A (en) * | 2016-08-17 | 2016-12-07 | 金乐仙 | A kind of normal pressure pure oxygen reactor and method |
| CN107935654B (en) * | 2017-11-30 | 2020-09-29 | 重庆蕊福农食用菌种植有限公司 | Fermentation equipment for producing organic fertilizer by using mushroom dregs |
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- 2009-03-06 CN CN2009100471297A patent/CN101496574B/en not_active Expired - Fee Related
Patent Citations (3)
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| CN2229458Y (en) * | 1995-03-31 | 1996-06-19 | 清华紫光(集团)总公司 | Belt solid fermentation reaction apparatus |
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