CN201439526U - Multifunctional liquid strain culture apparatus - Google Patents

Multifunctional liquid strain culture apparatus Download PDF

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Publication number
CN201439526U
CN201439526U CN 200920150186 CN200920150186U CN201439526U CN 201439526 U CN201439526 U CN 201439526U CN 200920150186 CN200920150186 CN 200920150186 CN 200920150186 U CN200920150186 U CN 200920150186U CN 201439526 U CN201439526 U CN 201439526U
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China
Prior art keywords
tank body
full
service fluid
culture device
cover
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Expired - Fee Related
Application number
CN 200920150186
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Chinese (zh)
Inventor
罗连富
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Individual
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Individual
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Priority to CN 200920150186 priority Critical patent/CN201439526U/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/52Mobile; Means for transporting the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure

Abstract

The utility model relates to a multifunctional liquid strain culture apparatus, which belongs to the field of microorganism strain culture and is particularly suitable for the culture of edible fungus liquid strains. The culture apparatus mainly consists of a pot cover, a pot body, a conical pot bottom and a truncated cone-shaped inner sleeve, wherein the pot cover is internally provided with a fine filter; a lifting shaft is arranged on the pot cover and is connected with a bracket shaft seat of the pot body through a steering bracket; the inside of an interlayer formed by the truncated cone-shaped inner sleeve is filled with water and is provided with a heater; the upper part of the interlayer is provided with a conduit connected with the pot body; the bottom in the pot is provided with a jet pipe which is connected with the fine filter through a tube; and the lower part of the pot body is provided with a landing leg socket which is connected with a landing leg. The utility model has the advantages of convenient conveyance, movement and operation, thorough sterilization and consistent quality of the strains, and can be used as a steam pressure disinfector, a drying box and a constant-temperature culture apparatus.

Description

Full-service fluid spawn culture device
Technical field
The utility model relates to the full-service fluid spawn culture device that the culture apparatus that the microbial liquid bacterial classification is produced, particularly edible fungi liquid strain are produced, and this equipment is widely used in all kinds of bacterium factory, bio-pharmaceuticals, Microbiological Lab.
Background technology
At present, known strain cultivation device (fermentor tank) is that formations such as culture tank, air feed, filtration, stirring, heating cooling, temperature control system are arranged.Wherein tank body is not too high, is exactly that the design of inner stirring system is complicated, causes culture precipitation, absorption, stops up, easy cleaning not, and make the bacterium culturing process simultaneously: quality differs before and after the bacterial classification.Filtering system is not independent sterilization, puts the side exactly, causes complicated operation when sterilizing simultaneously, and it is not thorough to sterilize.Cover is opened mobile dumb, causes inconvenient operation, and the fixing welding of tank body support can not be dismantled, and causes transportation inconvenience.The well heater utilization ratio, the sexual valence ratio has increased user's production cost.
Summary of the invention
In order to overcome all deficiencies of prior art, the purpose of this utility model provides a kind of convenient transportation, and conveniently moving is easy to operate, and is simple in structure, and sterilization is thorough, the strain cultivation device of strain quality self-consistentency.This incubator also can be made the vapor pressure sterilizer simultaneously, loft drier, and Constant Temperature Incubators uses.
The technical scheme that the utility model adopted is: full-service fluid spawn culture device is to be made of controlling box, strainer, compressor and culture tank.Controlling box connects compressor and well heater and transmitter respectively, and compressor is equal to fine filter by the road and connects.Well heater, fine filter, transmitter are fixed on the culture tank, and its major technique feature is: culture tank is by sealed detachable cap, and cylindrical tank, the conical jar end,, chuck constitutes in the truncated cone-shaped.Lid is built-in with fine filter, and its shell is fixed on the cover, because of fine filter places a jar inner top temperature highest point, can fully sterilize to it to the nutrient solution sterilization while.Cover is provided with cover lifting shaft device, and its beam warp axle sleeve connects in turning rack, and the turning rack lower end connects with the support axle bed on the tank body.Interior chuck is that truncated cone-shaped top connects in tank body 4/5 eminence, and radius 2/3 place at the bottom of tapered shaped can, bottom connects.In the middle part of spray tube is fixed at the bottom of the tapered shaped can, its nozzle is a 45, spout is towards interior chuck bottom, spray tube connects with fine filter through flexible pipe, and by at the bottom of chuck, the tapered shaped can in the truncated cone-shaped and the spray tube acting in conjunction, nutrient solution forms a round-robin fluid under inflation situation, gas, liquid, thing, three-phase material mix, dissolved oxygen is abundant, has avoided nutrient solution absorption, precipitation, the generation of latch up phenomenon.By adorning recirculated water in the interlayer that chuck and tank body constituted in the truncated cone-shaped, under it, water-in is set, the steam duct that links to each other with tank body is set at an upper portion thereof.Steam duct is provided with control valve, safety valve, tensimeter, outlet valve.1/5 place, described tank body bottom is provided with the well heater interface, 1/2 place is provided with sensor interface, control this interlayer temperature can carry out constant temperature culture, again can heat up the sterilization and the drying that can heat up, described tank body bottom is provided with three supporting leg sockets, it connects with supporting leg by the pin of living, steering wheel is housed on the supporting leg, and this device is convenient to dismounting, convenient transportation.Also be provided with on the tank body stopple coupon and on visor, cover be provided with the inoculation mouthful.
The beneficial effect that the utility model reached is, convenient transportation, and conveniently moving, easy to operate, sterilization thoroughly stirs strain quality self-consistentency, a tractor serves several purposes.
Description of drawings
Accompanying drawing is the utility model structural representation
In the drawings: 1, spiral lifting axle.2, turning rack; 3, vapor cock; 4, safety valve; 5, inoculation mouth; 6, turning rack axle bed; 7, go up visor; 8, tank body; 9, transmitter; 10, stopple coupon; 11, well heater; 12, removable pin; 13, supporting leg socket; 14, rack leg; 15, water intaking valve; 16, universal wheel; 17, control valve; 18, fine filter; 19, loam cake; 20, tensimeter; 21, safety valve; 22, tensimeter; 23, steam duct; 24, control valve; 25, outlet valve; 26, chuck in the garden platform shape; 27, interlayer; 28, well heater; 29, flexible pipe; 30, spray tube; 31, at the bottom of the tapered shaped can; 32, inoculation valve; 33, lifting axle sleeve; 34, shaft-cup connective slot.
Embodiment
The utility model is described in further detail below in conjunction with accompanying drawing
Press shown in the accompanying drawing: whole incubator is by cylindrical tank 8, and 31 4 major parts are formed at the bottom of interior chuck 26 of loam cake 19 and truncated cone-shaped and the tapered shaped can.Loam cake is connected by flange seal with tank body, quick-opening device.Chuck 26 tops are in tank body 1/5th eminence sealed attachment in the truncated cone-shaped, chuck 26 bottoms 2/3rds place's sealed attachment outside radius at the bottom of the tapered shaped can in the truncated cone-shaped.Interior chuck 26 constitutes at the bottom of interlayer 27 tapered shaped cans fixedly spray tube 30 of central part jointly with tank body, and injection nozzles is miter angle, and the spout direction connects flexible pipe 29 spray tube 30 is connected with fine filter in the loam cake 18 towards interior chuck 26 bottoms.Fine filter 18 is fixed in the loam cake 19 by shell, and spiral lifting axle 1 lower end flexibly connects by shaft-cup connective slot 34 and loam cake, and the upper end axle sleeve 33 that turns shape support 2 is connected with the spiral lifting axle, turns shape support lower end and connects with tank body upper bracket axle bed 6.
When the switch loam cake, rotation and lifting axle 1 loam cake 19 can be done vertical shifting up and down, moves but swinging strut 2 loam cakes 19 left and right sides are parallel.Tank body 8 is provided with visor 7, stopple coupon 10, and transmitter 9 and well heater 11 and well heater 28, well heater 11 and well heater 28 are inclined in the interlayer.By leading to outward in the jar, steam duct 23 is provided with control valve 24. safety valve 21, tensimeter 22, outlet valve 25. by steam duct 23 on interlayer top
The tank body bottom is provided with three supporting leg sockets 13, and the shape supporting leg 14 that turns that has universal wheel 16 is pegged graft with the supporting leg socket by connecting the pin 12 of living, and supporting leg can be dismantled flexibly, convenient transportation.
When nutrient solution is sterilized, open control valve 24, close all valves, the temperature control system of operation control box is started working well heater 11 and well heater 28, steam enter in the jar by conduit 23 in the interlayer 27, and to the nutrient solution sterilization that heats up, timing time arrives jointly for interlayer and steam, closing control valve 24 feeds recirculated water in interlayer 27 and by outlet valve 25 water outlets by water intaking valve 15, cooling.
When nutrient solution is cultivated, open valve 17 operation control box airing systems, gas by flexible pipe Guide spray pipe 30, imports in the nutrient solution aseptic gas by fine filter 18, and Open valve 3 nutrient solutions are along conical surface and circulate under barotropic condition.
When needing in the production process to carry out solid culture, Open valve 3 is closed all valves, and the operation control box temperature controlling system carries out constant temperature culture by interlayer to culture, plays the incubator effect.When needing article such as vessel dry in the production process, open valve 3 valves 17, valve 32, the operation temperature controlling system is by interlayer, intensification, and the operation airing system is dried by air feed, can carry out drying to article in the jar again, plays the loft drier effect.

Claims (8)

1. full-service fluid bacterium incubator, it is characterized in that: chuck in the truncated cone-shaped is set in culture tank, interlayer is provided with conduit outward and feeds in the jar, be provided with fine filter in the cover, cover is provided with lifting shaft, and tank body is provided with and turns to axle bed, and a jar inner bottom part is provided with spray tube, three supporting leg sockets are established in the tank body bottom, and the well heater interface is in the tank body bottom.
2. full-service fluid spawn culture device according to claim 1, it is characterized in that: interior chuck is a truncated cone-shaped, and top connects with tank body, the bottom connects at the bottom of with tapered shaped can.
3. full-service fluid spawn culture device according to claim 1 is characterized in that: interlayer outer catheter (23) is provided with outlet valve (25), control valve (24), tensimeter (22), safety valve (21).
4. full-service fluid spawn culture device according to claim 1 is characterized in that: be provided with fine filter in the cover.
5. full-service fluid spawn culture device according to claim 1, it is characterized in that: lifting shaft is a spirrillum on the cover, the bottom is fixed in the axial trough (34) on the cover, and screw shaft connects with the axle sleeve on the bogie (33), and the bogie bottom connects with tank body top bogie axle bed (6).
6. according to requiring 1 described full-service fluid spawn culture device in the right, it is characterized in that: a jar end is taper, and its top spray tube nozzle is a miter angle, and spout overlaps the bottom in truncated cone-shaped, and spray tube is connected with fine filter by flexible pipe.
7. full-service fluid spawn culture device according to claim 1 is characterized in that: the tank body bottom is provided with three supporting leg sockets, and socket is provided with and connects the pin (12) of living, and turns shape supporting leg (14) and has universal wheel (16).
8. full-service fluid spawn culture device according to claim 1, it is characterized in that: well heater is arranged on the tank body bottom, in the oblique feeding interlayer.
CN 200920150186 2009-04-26 2009-04-26 Multifunctional liquid strain culture apparatus Expired - Fee Related CN201439526U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 200920150186 CN201439526U (en) 2009-04-26 2009-04-26 Multifunctional liquid strain culture apparatus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 200920150186 CN201439526U (en) 2009-04-26 2009-04-26 Multifunctional liquid strain culture apparatus

Publications (1)

Publication Number Publication Date
CN201439526U true CN201439526U (en) 2010-04-21

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105087365A (en) * 2014-05-23 2015-11-25 大连富智森生物科技有限公司 Edible fungus liquid strain intelligent automatic culture machine
CN106258496A (en) * 2016-10-21 2017-01-04 成都天绿菌业有限公司 A kind of edible fungi sterilization tank
CN106497764A (en) * 2016-10-21 2017-03-15 成都天绿菌业有限公司 A kind of edible fungus species disinfection fermentation tank
CN106508425A (en) * 2016-10-21 2017-03-22 成都天绿菌业有限公司 Mechanically-opened type sterilization tank for edible fungus strains
CN106754409A (en) * 2016-12-08 2017-05-31 段必儒 White ginseng bacterium efficiently educates plant method
CN107058089A (en) * 2017-03-24 2017-08-18 大连富森智能科技有限公司 Liquid spawn fermentation tank
CN109880734A (en) * 2019-03-30 2019-06-14 中国水产科学研究院黑龙江水产研究所 Amino acid extraction element machine extracting method in a kind of flesh of fish

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105087365A (en) * 2014-05-23 2015-11-25 大连富智森生物科技有限公司 Edible fungus liquid strain intelligent automatic culture machine
CN106258496A (en) * 2016-10-21 2017-01-04 成都天绿菌业有限公司 A kind of edible fungi sterilization tank
CN106497764A (en) * 2016-10-21 2017-03-15 成都天绿菌业有限公司 A kind of edible fungus species disinfection fermentation tank
CN106508425A (en) * 2016-10-21 2017-03-22 成都天绿菌业有限公司 Mechanically-opened type sterilization tank for edible fungus strains
CN106754409A (en) * 2016-12-08 2017-05-31 段必儒 White ginseng bacterium efficiently educates plant method
CN106754409B (en) * 2016-12-08 2020-01-10 段必儒 Method for cultivating and planting holothuria leucorrhoeae
CN107058089A (en) * 2017-03-24 2017-08-18 大连富森智能科技有限公司 Liquid spawn fermentation tank
CN109880734A (en) * 2019-03-30 2019-06-14 中国水产科学研究院黑龙江水产研究所 Amino acid extraction element machine extracting method in a kind of flesh of fish

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C14 Grant of patent or utility model
GR01 Patent grant
C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20100421

Termination date: 20110426