CN101413026A - Biological enzyme (film) antibacterial neutralization agent - Google Patents
Biological enzyme (film) antibacterial neutralization agent Download PDFInfo
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- CN101413026A CN101413026A CNA200710113239XA CN200710113239A CN101413026A CN 101413026 A CN101413026 A CN 101413026A CN A200710113239X A CNA200710113239X A CN A200710113239XA CN 200710113239 A CN200710113239 A CN 200710113239A CN 101413026 A CN101413026 A CN 101413026A
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- neutralizing agent
- biological enzyme
- film
- antibacterial
- disinfection
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Abstract
The invention relates to an antibacterial neutralizing agent for biological enzyme or a biological film, wherein main components are sodium thiosulfate, lecithin, tween-80, a genuine soap, and nutritional broth which are mixed according to the proportion to prepare the antibacterial neutralizing agent through a special process. The antibacterial neutralizing agent is suitable for evaluating the disinfection effect of biological enzyme (film) disinfection products in disinfection experiments. The antibacterial neutralizing agent has the advantages that the antibacterial neutralizing agent has feasible and reliable neutralizing effect on the biological enzyme disinfection products; products obtained by the neutralizing agent which reacts with the biological enzyme disinfection products have no functions of killing experimental microorganisms and inhibiting the growth of the experimental microorganisms; and the antibacterial neutralizing agent has no destroying effect on culture compositions and also does not affect the physical properties of the culture compositions.
Description
Technical field the present invention relates to the antibiotic neutralizing agent of a kind of biological enzyme (film), and its main component is that Sulfothiorine, Yelkin TTS, soil temperature-80, soap base, nutrient broth are mixed in proportion into mixture.The key problem of disinfection experiment is to estimate sterilization, antibacterial, the anti-microbial effect of disinfection factor.Estimate the effect of sterilizing agent, must strictly grasp action time.Sterilizing agent and microorganism touch after the preset time, must remove its effect immediately, so just can accurately obtain the data of sterilizing agent action effect; Simultaneously must not contain remaining sterilizing agent in the sample after taking to sterilize.To prevent that in recover cultivating microorganism from continuing to be subjected to the effect of sterilizing agent.Therefore, the experiment of any one evaluating chemical sterilizing agent all must have the reliable method of removing remaining sterilizing agent.Do not adopt the experiment of removing remaining sterilizing agent measure, should think serious mistake in design, the result who draws according to such experiment is insecure.The chemistry neutralisation is the sterilizing agent method of at present the most frequently used removal remnants, and its advantage is that method is simple, and is easy to use, reliable for effect.The antibiotic neutralizing agent of biological enzyme (film) can be used for estimating the disinfection of biological enzyme (film) class thimerosal.Existing thimerosal neutralizing agent kind is a lot, and common have acid, alkali, absorption, dilution, centrifugal a, filtration etc.The above-mentioned screening of all passing through strictness also can't not determined its working concentration; Corresponding sterilizing agent do not had solid neutralizing effect; Neutralizing agent itself or with the product of sterilizing agent reaction the experiment microorganism is had killing or suppress the effect of its growth; To cultivating composition destruction is arranged, also influence its physical behavior.Along with the sterilization progress of research, both at home and abroad to the research of neutralizing agent also in continuous development.The disinfection person wishes to have always and a kind ofly can overcome the insufficient neutralizing agent of above-mentioned neutralizing agent.
Background technology the object of the present invention is to provide and a kind ofly can overcome the insufficient compound neutralizing agent of existing sterilization neutralizing agent---the antibiotic neutralizing agent of biological enzyme (film), compares with other class neutralizing agent to possess following characteristics: corresponding biological enzyme (film) class sterilizing agent is had solid neutralizing effect; Neutralizing agent itself or with the product of biological enzyme (film) class sterilizing agent reaction the experiment microorganism is not had killing or suppress the effect of its growth; Do not have destruction to cultivating composition, also do not influence its physical behavior.
Summary of the invention main points of the present invention are to select suitable component, and rationally are mixed, through heat, dissolve, technology such as mixing, cooling, packing, high-temperature sterilization forms a kind of biological enzyme (film) class thimerosal neutralizing agent.
The prescription following (component and weight percent % thereof) that the present invention selects: Sulfothiorine (0.1-0.5), Yelkin TTS (0.1-0.3), soil temperature-80 (0.1-0.3), soap base (0.1-0.3), nutrient broth (20-30), purifying distilled water add to 100.
Sulfothiorine, Yelkin TTS, soil temperature-80, soap base, meat soup can with biological enzyme (film) the class thimerosal generation chemical reaction of remnants, generate to the experiment microorganism do not kill, suppress its growth effect, do not have destruction to cultivating composition, physical behavior not have the neutralized reaction product that influences.Corresponding biological enzyme (film) class sterilizing agent had solid neutralizing effect.
The preparation method of product of the present invention is:
1, nutrient broth is heated, is dissolved configuration in proportion;
2, soap base is carried out in proportion, heats dissolving;
3, Sulfothiorine, soil temperature-80, Yelkin TTS are dissolved in proportion 54 ℃ of the pure middle heating of the two aquae destillatas of purifying, cooling;
3, dissolved soap base, nutrient broth are joined by proportioning in the mixing solutions such as cooled Sulfothiorine, remix evenly, carry out packing after the cooling, 105 ℃ of sterilizations 15 minutes.Steriling test, warehousing after passing.
The present invention has the following advantages: corresponding biological enzyme (film) class sterilizing agent is had solid neutralizing effect; The product of neutralizing agent itself and the reaction of biological enzyme (film) class sterilizing agent is to testing the effect that microorganism does not have killing, suppresses its growth; Do not have destruction to cultivating composition, also do not influence its physical behavior, it is clear to count.
The present invention is described in further detail below in conjunction with embodiment for embodiment:
Be configured according to following table institute column data (weight percent %) and described step thereof:
Prescription one:
Sulfothiorine 0.1-0.5
Yelkin TTS 0.1-0.3
Soil temperature-80 0.1-0.3
Soap base 0.1-0.3
Nutrient broth 20.0-30.0
The two aquae destillatas of purifying add to 100
Prescription two:
Sulfothiorine 0.1-0.3
Yelkin TTS 0.1-0.2
Soil temperature-80 0.1-0.2
Soap base 0.1-0.2
Nutrient broth 20.0-25.0
The two aquae destillatas of purifying add to 100
Prescription three:
Sulfothiorine 0.2-0.5
Yelkin TTS 0.2-0.3
Soil temperature-80 0.2-0.3
Soap base 0.2-0.3
Nutrient broth 22.0-30.0
The two aquae destillatas of purifying add to 100
Prescription four:
Sulfothiorine 0.1-0.4
Yelkin TTS 0.2-0.3
Soil temperature-80 0.1-0.2
Soap base 0.1-0.3
Nutrient broth 20.0-30.0
The two aquae destillatas of purifying add to 100.
Claims (2)
1, the antibiotic neutralizing agent of a kind of biological enzyme of the present invention (film) is sterilization, antibacterial, the anti-microbial effect that is used for disinfection experiment, estimates biological enzyme (film) class sterilizing agent disinfection factor; It is characterized in that having following prescription (component and weight percent % thereof): Sulfothiorine (0.1-0.5), Yelkin TTS (0.1-0.3), soil temperature-80 (0.1-0.3), soap base (0.1-0.3), nutrient broth (20-30), purified water add to 100;
2, the preparation method of the antibiotic neutralizing agent of the described biological enzyme of a kind of claim 1 (film) is characterized in that having following step:
1), nutrient broth is heated, is dissolved configuration in proportion;
2), soap base is carried out in proportion, heats dissolving;
3), Sulfothiorine, soil temperature-80, Yelkin TTS are dissolved in 54 ℃ of the pure middle heating of the two aquae destillatas of purifying, cooling in proportion;
4), dissolved soap base, nutrient broth joined by proportioning in the mixing solutions such as cooled Sulfothiorine, remix evenly, carry out packing after the cooling, 105 ℃ of sterilizations 15 minutes.Steriling test, warehousing after passing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200710113239XA CN101413026A (en) | 2007-10-15 | 2007-10-15 | Biological enzyme (film) antibacterial neutralization agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200710113239XA CN101413026A (en) | 2007-10-15 | 2007-10-15 | Biological enzyme (film) antibacterial neutralization agent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101413026A true CN101413026A (en) | 2009-04-22 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA200710113239XA Pending CN101413026A (en) | 2007-10-15 | 2007-10-15 | Biological enzyme (film) antibacterial neutralization agent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101413026A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103194527A (en) * | 2012-01-05 | 2013-07-10 | 曲剑英 | Public area hygiene testing effect evaluation improved sampling reagent |
-
2007
- 2007-10-15 CN CNA200710113239XA patent/CN101413026A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103194527A (en) * | 2012-01-05 | 2013-07-10 | 曲剑英 | Public area hygiene testing effect evaluation improved sampling reagent |
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| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20090422 |