CN101029325A - Neutralizing nutrient broth medium - Google Patents
Neutralizing nutrient broth medium Download PDFInfo
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- CN101029325A CN101029325A CN 200610042538 CN200610042538A CN101029325A CN 101029325 A CN101029325 A CN 101029325A CN 200610042538 CN200610042538 CN 200610042538 CN 200610042538 A CN200610042538 A CN 200610042538A CN 101029325 A CN101029325 A CN 101029325A
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- nutrient broth
- sodium
- broth medium
- neutralizing
- tryptones
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Abstract
A neutralized nutrient broth culture medium consists of polysorhol-80, lecithin, sodium hyposulfite, beef grease, trypsatone and sodium chloride. The process is carried out by mixing proportionally, chemical reacting lecithin, polysorhol-80 and sodium hyposulfite with residual Chinese-herb disinfectants and neutralizing. It has better neutralizing function, no inhibition for microbe and destructive role for nutrients. It can be used for Chinese-herb disinfectants culture medium.
Description
Technical field the present invention relates to a kind of in and nutrient broth medium, its main component is that polysorbate-80, Yelkin TTS, Sulfothiorine, extractum carnis, Tryptones, sodium-chlor are mixed in proportion and form.The key problem of disinfection experiment is to estimate the sterilization and the bacteriostatic action of disinfection factor.The effect of evaluating chemical sterilizing agent must strictly be grasped action time.Sterilizing agent and microorganism touch after the preset time, must remove its effect immediately, so just can accurately obtain the data of sterilizing agent action effect; Simultaneously must not contain remaining sterilizing agent in the sample after taking to sterilize.To prevent that in recover cultivating microorganism from continuing to be subjected to the effect of sterilizing agent.Therefore, do not adopt the experiment of removing remaining sterilizing agent measure, should think serious mistake in design, the result who draws according to such experiment is insecure.The chemistry neutralisation is the sterilizing agent method of at present the most frequently used removal remnants, and its advantage is that method is simple, and is easy to use, reliable for effect.In and nutrient broth medium can be used for estimating microbial culture after the disinfection of herbal medicine thimerosal.Existing thimerosal neutralizing agent kind is a lot, and common have acid, alkali, absorption, dilution, centrifugal a, filtration etc.The above-mentioned screening of all passing through strictness also can't not determined its working concentration; Corresponding sterilizing agent do not had solid neutralizing effect; Neutralizing agent itself or the experiment microbial bacterial is cultivated the effect that killing is arranged or suppress its growth with the product of sterilizing agent reaction; To cultivating composition destruction is arranged, also influence its physical behavior.Along with the sterilization progress of research, both at home and abroad to the research of neutralizing agent also in continuous development.The disinfection person wish to have always a kind of can overcome above-mentioned neutralizing agent insufficient in and nutrient broth medium.
Background technology the object of the present invention is to provide a kind of can overcome existing sterilization neutralizing agent insufficient in and nutrient broth medium.Compare with other class neutralizing agent and to possess following characteristics: corresponding herbal medicine thimerosal is had solid neutralizing effect; In and the product of nutrient broth medium itself and sterilizing agent reaction, to testing the effect that microorganism does not have killing or suppresses its growth; Do not have destruction to cultivating composition, also do not influence its physical behavior; The experiment bacterium not only increases the bacterium breeding in this substratum, and the residual disinfectancy agent obtains corresponding neutralization.
Summary of the invention main points of the present invention are to select suitable component, and rationally are mixed, through heat, dissolve, technology such as mixing, cooling, packing, high-temperature sterilization form a kind of in and nutrient broth medium.
The prescription following (component and weight percent % thereof) that the present invention selects: Yelkin TTS (0.1-0.3); Polysorbate-80 (0.5-1.0); Sulfothiorine (0.1-1.0); Extractum carnis (0.5-0.6); Tryptones (1.0-1.5); Sodium-chlor (0.5-0.6); Distilled water adds to 100.
Yelkin TTS, polysorbate-80, Sulfothiorine can with the herbal medicine thimerosal generation chemical reaction of remnants, generate to the experiment microorganism do not kill or suppress its growth effect, do not have destruction to cultivating composition, physical behavior not have the neutralized reaction product that influences.Corresponding herbal medicine thimerosal had solid neutralizing effect.Extractum carnis, Tryptones, sodium-chlor be in and the nutrition base fluid in the nutrient broth medium, the experiment microorganism not only increases bacterium breeding in this substratum, and the residual disinfectancy agent obtains corresponding neutralization.
The preparation method of product of the present invention is:
1), polysorbate-80, Sulfothiorine, Yelkin TTS are heated in proportion, dissolved.
2), extractum carnis, Tryptones, sodium-chlor are dissolved in heating for dissolving in the distilled water in proportion, cooling.
3), with dissolved 1) mixed solution joins cooled 2) in the solution, mix, the cooling back transfers PH6.1-6.3.
4), after the packing, 115 ℃ of pressuresteam sterilizations, 20-30min.
The present invention has the following advantages: (1) has solid neutralizing effect to corresponding herbal medicine thimerosal; (2) product that reacts with nutrient broth medium itself and sterilizing agent in is to testing the effect that microorganism does not have killing or suppresses its growth; (3) do not have destruction to cultivating composition, also do not influence its physical behavior; (4) the experiment bacterium not only increases the bacterium breeding in this substratum, and the residual disinfectancy agent obtains corresponding neutralization.
The present invention is described in further detail below in conjunction with embodiment for embodiment:
Be configured according to following table institute column data (weight percent %) and described step thereof:
Prescription one:
Yelkin TTS 0.1-0.3
Polysorbate-80 0.5-1.0
Sulfothiorine 0.1-1.0
Extractum carnis 0.5-0.6
Tryptones 1.0-1.5
Sodium-chlor 0.5-0.6
Distilled water adds to 100
Prescription two:
Yelkin TTS 0.2-0.3
Polysorbate-80 0.6-1.0
Sulfothiorine 0.5-1.0
Extractum carnis 0.5-0.6
Tryptones 1.3-1.5
Sodium-chlor 0.5-0.6
Distilled water adds to 100
Prescription three:
Yelkin TTS 0.1-0.2
Polysorbate-80 0.5-0.8
Sulfothiorine 0.1-0.7
Extractum carnis 0.5-0.6
Tryptones 1.0-1.4
Sodium-chlor 0.5-0.6
Distilled water adds to 100
Prescription four:
Yelkin TTS 0.2-0.3
Polysorbate-80 0.6-0.7
Sulfothiorine 0.5-0.8
Extractum carnis 0.5-0.6
Tryptones 1.3-1.4
Sodium-chlor 0.5-0.6
Distilled water adds to 100
。
Claims (2)
1, a kind of with polysorbate-80, Yelkin TTS, Sulfothiorine, extractum carnis, Tryptones, sodium-chlor be main ingredient in and nutrient broth medium.It is characterized in that having following prescription (component and weight percent % thereof): Yelkin TTS (0.1-0.3); Polysorbate-80 (0.5-1.0); Sulfothiorine (0.1-1.0); Extractum carnis (0.5-0.6); Tryptones (1.0-1.5); Sodium-chlor (0.5-0.6); Distilled water adds to 100.
2, a kind of claim 1 described in and the preparation method of nutrient broth medium, it is characterized in that having following step:
1), polysorbate-80, Sulfothiorine, Yelkin TTS are heated in proportion, dissolved.
2), extractum carnis, Tryptones, sodium-chlor are dissolved in heating for dissolving in the distilled water in proportion, cooling.
3), with dissolved 1) mixed solution joins cooled 2) in the solution, mix, the cooling back transfers PH6.1-6.3.
4), after the packing, 115 ℃ of pressuresteam sterilizations, 20-30min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610042538 CN101029325A (en) | 2006-02-27 | 2006-02-27 | Neutralizing nutrient broth medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610042538 CN101029325A (en) | 2006-02-27 | 2006-02-27 | Neutralizing nutrient broth medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101029325A true CN101029325A (en) | 2007-09-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610042538 Pending CN101029325A (en) | 2006-02-27 | 2006-02-27 | Neutralizing nutrient broth medium |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105177106A (en) * | 2015-09-06 | 2015-12-23 | 北京陆桥技术有限责任公司 | Chlorine-containing disinfectant resistant bacteria count culture medium |
-
2006
- 2006-02-27 CN CN 200610042538 patent/CN101029325A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105177106A (en) * | 2015-09-06 | 2015-12-23 | 北京陆桥技术有限责任公司 | Chlorine-containing disinfectant resistant bacteria count culture medium |
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