CN101698866A - Broth glucose neutralization sampling solution - Google Patents
Broth glucose neutralization sampling solution Download PDFInfo
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- CN101698866A CN101698866A CN200710115726A CN200710115726A CN101698866A CN 101698866 A CN101698866 A CN 101698866A CN 200710115726 A CN200710115726 A CN 200710115726A CN 200710115726 A CN200710115726 A CN 200710115726A CN 101698866 A CN101698866 A CN 101698866A
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- glucose
- sampling solution
- disinfectant
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- neutralization
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Abstract
The invention relates to broth glucose neutralization sampling solution. The broth glucose neutralization sampling solution is prepared by mixing main components of Tween-80, lecithin, sodium hyposulfite, glucose and tryptone in a certain ratio. The broth glucose neutralization sampling solution can be used for evaluating the hyperplasia culture of a fungi liquid medium after the disinfection of a compound disinfectant. The lecithin, the Tween-80 and the sodium hyposulfite can chemically react with the residual compound disinfectant to generate neutralized products which do not have the effects of killing laboratory microorganisms or inhibiting the growth of test microorganisms, damaging cultured components, or affecting physical characteristics. The broth glucose neutralization sampling solution has the concrete and reliable neutralizing effect on the compound disinfectant. The glucose and the tryptone are the nutrient substrate of the broth glucose neutralization sampling solution, and the laboratory fungi are enriched and bred in the liquid culture medium, and the residual disinfectant is also correspondingly neutralized. The sampling solution has the advantages that: (1) the sampling solution is a liquid neutralizing culture medium, and has the concrete and reliable effect of neutralizing the corresponding compound disinfectant; (2) the product generated when the sampling solution reacts with the disinfectant does not have the effect of killing the test microorganisms or inhibiting the growth of test microorganisms; (3) the product generated when the sampling solution reacts with the disinfectant does not have the effect of damaging the cultured components, or affecting the physical characteristics; and (4) the laboratory fungi are enriched and bred in the liquid culture medium, and the residual disinfectant is also correspondingly neutralized.
Description
Technical field the present invention relates in a kind of broth glucose and sample solution, and its main component is that soil temperature-80, Yelkin TTS, Sulfothiorine, glucose, Tryptones are mixed in proportion and form.The key problem of disinfection experiment is to estimate the sterilization and the bacteriostatic action of disinfection factor.Estimate the effect of compound chemical sterilizing agent, must strictly grasp action time.Compound chemical sterilizing agent and microorganism touch after the preset time, must remove its effect immediately, so just can accurately obtain the data of sterilizing agent action effect; Simultaneously must not contain remaining compound chemical sterilizing agent in the sample after taking to sterilize.To prevent that in recover cultivating microorganism from continuing to be subjected to the effect of sterilizing agent.Therefore, do not adopt the experiment of removing remaining sterilizing agent measure, should think serious mistake in design, the result who draws according to such experiment is insecure.The chemistry neutralisation is the sterilizing agent method of at present the most frequently used removal remnants, and its advantage is that method is simple, and is easy to use, reliable for effect.In the broth glucose and sample solution can be used for estimating in the fungi liquid after the compound disinfectant disinfection and the cultivation of sample solution.Existing thimerosal neutralizing agent kind is a lot, and common have acid, alkali, absorption, dilution, centrifugal a, filtration etc.The above-mentioned screening of all passing through strictness also can't not determined its working concentration; Corresponding sterilizing agent do not had solid neutralizing effect; Neutralizing agent itself or the experiment microbial bacterial is cultivated the effect that killing is arranged or suppress its growth with the product of sterilizing agent reaction; To cultivating composition destruction is arranged, also influence its physical behavior.Along with the sterilization progress of research, both at home and abroad to the research of neutralizing agent also in continuous development.The disinfection person wishes to have always and a kind ofly can overcome the insufficient neutralizing agent of above-mentioned neutralizing agent----in the broth glucose and sample solution.
Background technology the object of the present invention is to provide and a kind ofly can overcome in the insufficient broth glucose of existing sterilization neutralizing agent and sample solution.Compare with other class neutralizing agent and to possess following characteristics: corresponding compound disinfectant is had solid neutralizing effect; The product that reacts with sample solution itself and sterilizing agent in the broth glucose is to testing the effect that microorganism does not have killing or suppresses its growth; Do not have destruction to cultivating composition, also do not influence its physical behavior; The experiment fungi not only increases the bacterium breeding in this liquid nutrient medium, and the residual disinfectancy agent obtains corresponding neutralization.
Summary of the invention main points of the present invention are to select suitable component, and rationally are mixed, through heat, dissolve, technology such as mixing, cooling, packing, high-temperature sterilization forms in a kind of broth glucose and sample solution.
The prescription following (component and weight percent % thereof) that the present invention selects: Yelkin TTS (0.1-0.3); Soil temperature-80 (0.5-1.0); Sulfothiorine (0.1-1.0); Glucose (1.5-2.0); Tryptones (1.0-1.5); Distilled water adds to 100.
Yelkin TTS, soil temperature-80, Sulfothiorine can with the compound disinfectant generation chemical reaction of remnants, generate to the experiment microorganism do not kill or suppress its growth effect, do not have destruction to cultivating composition, physical behavior not have the neutralized reaction product that influences.Corresponding compound disinfectant had solid neutralizing effect.Glucose, Tryptones are in the broth glucose and the nutraceutical matrix in the sample solution, and the experiment fungi not only increases the bacterium breeding in this liquid nutrient medium, and the residual disinfectancy agent obtains corresponding neutralization.
The preparation method of product of the present invention is:
1), polysorbate-80, Sulfothiorine, Yelkin TTS are heated in proportion, dissolved.
2), with glucose, Tryptones in proportion heating for dissolving in distilled water, be chilled to 54 ℃.
3), with dissolved 2) mixed solution joins dissolved 2) in the solution, mix, the cooling back transfers PH6.1-6.3.
4), after the packing, 115 ℃ of pressuresteam sterilizations, 20-30min.
The present invention has the following advantages: (1) is in the liquid and substratum, and corresponding compound disinfectant is had solid neutralizing effect; (2) product that reacts with sample solution itself and sterilizing agent in the broth glucose is to testing the effect that microorganism does not have killing or suppresses its growth; (3) do not have destruction to cultivating composition, also do not influence its physical behavior; (4) the experiment fungi not only increases the bacterium breeding in this liquid nutrient medium, and the residual disinfectancy agent obtains corresponding neutralization.
The present invention is described in further detail below in conjunction with embodiment for embodiment:
Be configured according to following table institute column data (weight percent %) and described step thereof:
Claims (2)
1. the present invention relates in a kind of broth glucose and sample solution, its main component is that soil temperature-80, Yelkin TTS, Sulfothiorine, glucose, Tryptones are mixed in proportion and form; It is characterized in that having following prescription (component and weight percent % thereof): Yelkin TTS (0.1-0.3); Soil temperature-80 (0.5-1.0); Sulfothiorine (0.1-1.0); Glucose (1.5-2.0); Tryptones (1.0-1.5); Distilled water adds to 100.
2. in the described broth glucose of claim 1 and the preparation method of sample solution, it is characterized in that having following step:
1), polysorbate-80, Sulfothiorine, Yelkin TTS are heated in proportion, dissolved;
2), with glucose, Tryptones in proportion heating for dissolving in distilled water, be chilled to 54 ℃;
3), with dissolved 1) mixed solution joins dissolved 2) in the solution, mix, the cooling back transfers PH6.1-6.3;
4), after the packing, 115 ℃ of pressuresteam sterilizations, 20-30min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710115726A CN101698866A (en) | 2007-12-18 | 2007-12-18 | Broth glucose neutralization sampling solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710115726A CN101698866A (en) | 2007-12-18 | 2007-12-18 | Broth glucose neutralization sampling solution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101698866A true CN101698866A (en) | 2010-04-28 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200710115726A Pending CN101698866A (en) | 2007-12-18 | 2007-12-18 | Broth glucose neutralization sampling solution |
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| CN (1) | CN101698866A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107151688A (en) * | 2017-05-23 | 2017-09-12 | 新乡医学院 | In a kind of public sanitary place air sampling and culture medium |
-
2007
- 2007-12-18 CN CN200710115726A patent/CN101698866A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107151688A (en) * | 2017-05-23 | 2017-09-12 | 新乡医学院 | In a kind of public sanitary place air sampling and culture medium |
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| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20100428 |