CN101698865A - Neutralizing elution sample solution for chloral disinfection - Google Patents
Neutralizing elution sample solution for chloral disinfection Download PDFInfo
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- CN101698865A CN101698865A CN200710115725A CN200710115725A CN101698865A CN 101698865 A CN101698865 A CN 101698865A CN 200710115725 A CN200710115725 A CN 200710115725A CN 200710115725 A CN200710115725 A CN 200710115725A CN 101698865 A CN101698865 A CN 101698865A
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- chloral
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Abstract
The invention relates to neutralizing elution sample solution for chloral disinfection, which comprises the main components of poly-sorbitol-80, lecithin, sodium thiosulfate, glucose and tryptone which are mixed together in proportion. The neutralizing elution sample solution for the chloral disinfection can be used for evaluating the bacterial culture after chloral disinfection. The lecithin, the poly-sorbitol-80 and the sodium thiosulfate can perform chemical reaction with the residual chloral disinfection to generate a neutralization product which cannot kill or inhibit the growth of experimental microorganisms, has no destructive effect on culture components and has no influence on physical properties. The neutralizing elution sample solution has a practical and reliable neutralizing effect on a corresponding chloral disinfectant. The glucose and the tryptone are nutrition foundation solution in a glucose bouillon culture medium, and the experimental microorganisms in the culture medium not only increase the bacteria and propagate, but also correspondingly neutralize the residual chloral disinfectant.
Description
Technical field the present invention relates in a kind of chloral disinfection and elution sample solution, and its main component is that polysorbate-80, Yelkin TTS, Sulfothiorine, glucose, Tryptones are mixed in proportion and form.The key problem of disinfection experiment is to estimate the sterilization and the bacteriostatic action of disinfection factor.The effect of evaluating chemical chloral disinfection must strictly be grasped action time.Chloral disinfection liquid and microorganism touch after the preset time, must remove its effect immediately, so just can accurately obtain the data of chloral disinfection liquid action effect; Simultaneously must not contain remaining chloral disinfection liquid in the sample after taking to sterilize.To prevent that in recover cultivating microorganism from continuing to be subjected to the effect of chloral disinfection.Therefore, do not adopt the experiment of removing remaining chloral disinfection liquid measure, should think serious mistake in design, the result who draws according to such experiment is insecure.The chemistry neutralisation is the chloral disinfection liquid method of at present the most frequently used removal remnants, and its advantage is that method is simple, and is easy to use, reliable for effect.In the chloral disinfection and elution sample solution can be used for estimating microbial culture after the chloral disinfection disinfection.Existing thimerosal neutralizing agent kind is a lot, and common have acid, alkali, absorption, dilution, centrifugal a, filtration etc.The above-mentioned screening of all passing through strictness also can't not determined its working concentration; Corresponding chloral disinfection liquid do not had solid neutralizing effect; Neutralizing agent itself or the experiment microbial bacterial is cultivated the effect that killing is arranged or suppress its growth with the product of chloral disinfection liquid reaction; To cultivating composition destruction is arranged, also influence its physical behavior.Along with the sterilization progress of research, both at home and abroad to the research of neutralizing agent also in continuous development.The disinfection person wishes to have always and a kind ofly can overcome the insufficient neutralizing agent of above-mentioned neutralizing agent----in the chloral disinfection and elution sample solution.
Background technology the object of the present invention is to provide and a kind ofly can overcome in the insufficient chloral disinfection of existing sterilization neutralizing agent and elution sample solution.Compare with other class neutralizing agent and to possess following characteristics: corresponding chloral disinfection liquid is had solid neutralizing effect; The product that reacts with elution sample solution itself and chloral disinfection liquid in the chloral disinfection is to testing the effect that microorganism does not have killing or suppresses its growth; Do not have destruction to cultivating composition, also do not influence its physical behavior; The experiment fungi not only increases the bacterium breeding in this substratum, and residual chloral thimerosal obtains corresponding neutralization.
Summary of the invention main points of the present invention are to select suitable component, and rationally are mixed, through heat, dissolve, technology such as mixing, cooling, packing, high-temperature sterilization forms in a kind of chloral disinfection and elution sample solution.
The prescription following (component and weight percent % thereof) that the present invention selects: Yelkin TTS (0.1-0.3); Polysorbate-80 (0.5-1.0); Sulfothiorine (0.1-1.0); Glucose (1.5-2.0); Tryptones (1.0-1.5); Distilled water adds to 100.
Yelkin TTS, polysorbate-80, Sulfothiorine can with the chloral disinfection generation chemical reaction of remnants, generate to the experiment microorganism do not kill or suppress its growth effect, do not have destruction to cultivating composition, physical behavior not have the neutralized reaction product that influences.Corresponding chloral disinfection liquid had solid neutralizing effect.Glucose, Tryptones are the nutrition base fluid in the glucose broth, and the experiment microorganism not only increases the bacterium breeding in this substratum, and residual chloral thimerosal obtains corresponding neutralization.
The preparation method of product of the present invention is:
1), polysorbate-80, Sulfothiorine, Yelkin TTS are heated in proportion, dissolved.
2), glucose, Tryptones are dissolved in heating for dissolving in the distilled water in proportion, cooling.
3), with dissolved 1) mixed solution joins cooled 2) in the solution, mix, the cooling back transfers PH6.1-6.3.
4), after the packing, 115 ℃ of pressuresteam sterilizations, 20-30min.
The present invention has the following advantages: (1) has solid neutralizing effect to corresponding chloral disinfection liquid; (2) product that reacts with elution sample solution itself and chloral disinfection liquid in the chloral disinfection is to testing the effect that microorganism does not have killing or suppresses its growth; (3) do not have destruction to cultivating composition, also do not influence its physical behavior; (4) the experiment bacterium not only increases the bacterium breeding in this substratum, and residual chloral thimerosal obtains corresponding neutralization.
The present invention is described in further detail below in conjunction with embodiment for embodiment:
Be configured according to following table institute column data (weight percent %) and described step thereof:
Claims (2)
1. the present invention relates in a kind of chloral disinfection and elution sample solution, its main component is that polysorbate-80, Yelkin TTS, Sulfothiorine, glucose, Tryptones are mixed in proportion and form; It is characterized in that having following prescription (component and weight percent % thereof): Yelkin TTS (0.1-0.3); Polysorbate-80 (0.5-1.0); Sulfothiorine (0.1-1.0); Glucose (1.5-2.0); Tryptones (1.0-1.5); Distilled water adds to 100.
2. in the described chloral disinfection of claim 1 and the preparation method of elution sample solution, it is characterized in that having following step:
1), polysorbate-80, Sulfothiorine, Yelkin TTS are heated in proportion, dissolved;
2), glucose, Tryptones are dissolved in heating for dissolving in the distilled water in proportion, cooling;
3), with dissolved 1) mixed solution joins cooled 2) in the solution, mix, the cooling back transfers PH6.1-6.3;
4), after the packing, 115 ℃ of pressuresteam sterilizations, 20-30min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710115725A CN101698865A (en) | 2007-12-18 | 2007-12-18 | Neutralizing elution sample solution for chloral disinfection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710115725A CN101698865A (en) | 2007-12-18 | 2007-12-18 | Neutralizing elution sample solution for chloral disinfection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101698865A true CN101698865A (en) | 2010-04-28 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200710115725A Pending CN101698865A (en) | 2007-12-18 | 2007-12-18 | Neutralizing elution sample solution for chloral disinfection |
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| CN (1) | CN101698865A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105101776A (en) * | 2013-03-28 | 2015-11-25 | 诺维信生物农业公司 | Compositions and methods for enhancing microbial stability |
| CN115253144A (en) * | 2022-08-09 | 2022-11-01 | 青岛嘉智生物技术有限公司 | Neutralizing agent for glutaraldehyde disinfectant and preparation method thereof |
-
2007
- 2007-12-18 CN CN200710115725A patent/CN101698865A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105101776A (en) * | 2013-03-28 | 2015-11-25 | 诺维信生物农业公司 | Compositions and methods for enhancing microbial stability |
| CN105101776B (en) * | 2013-03-28 | 2019-04-02 | 诺维信生物农业公司 | For enhancing the composition and method of microbial stability |
| CN115253144A (en) * | 2022-08-09 | 2022-11-01 | 青岛嘉智生物技术有限公司 | Neutralizing agent for glutaraldehyde disinfectant and preparation method thereof |
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Open date: 20100428 |