CN101463377A - Biological enzyme neutralization agent - Google Patents
Biological enzyme neutralization agent Download PDFInfo
- Publication number
- CN101463377A CN101463377A CNA2007101157611A CN200710115761A CN101463377A CN 101463377 A CN101463377 A CN 101463377A CN A2007101157611 A CNA2007101157611 A CN A2007101157611A CN 200710115761 A CN200710115761 A CN 200710115761A CN 101463377 A CN101463377 A CN 101463377A
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- biological enzyme
- neutralizing agent
- sulfothiorine
- proportion
- soap base
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Abstract
The invention relates to a biological enzyme neutralizing agent. The main components comprise sodium thiosulfate, lecithin, tween-80, genuine soap and nutrient bouillon which are mixed in proportion and are processed to obtain the neutralizing agent by special technology. The biological enzyme neutralizing agent is suitable for evaluating the disinfection effect of a biological enzyme disinfection product in a disinfection experiment. The biological enzyme neutralizing agent has the advantages as follows: the biological enzyme neutralizing agent has practical and reliable neutralizing effect on the corresponding biological enzyme disinfection product; the neutralizing agent or a product produced in the reaction of the neutralizing agent and the biological enzyme disinfection product does not have effect of killing or restraining the growth of experimental microorganism; and the biological enzyme neutralizing agent does not damage the culture component and also does not affect the physical properties.
Description
Technical field the present invention relates to a kind of biological enzyme neutralizing agent, and its main component is that Sulfothiorine, Yelkin TTS, soil temperature-80, soap base, nutrient broth are mixed in proportion into mixture.The key problem of disinfection experiment is to estimate sterilization, antibacterial, the anti-microbial effect of disinfection factor.Estimate the effect of sterilizing agent, must strictly grasp action time.Sterilizing agent and microorganism touch after the preset time, must remove its effect immediately, so just can accurately obtain the data of sterilizing agent action effect; Simultaneously must not contain remaining sterilizing agent in the sample after taking to sterilize.To prevent that in recover cultivating microorganism from continuing to be subjected to the effect of sterilizing agent.Therefore, the experiment of any one evaluating chemical sterilizing agent all must have the reliable method of removing remaining sterilizing agent.Do not adopt the experiment of removing remaining sterilizing agent measure, should think serious mistake in design, the result who draws according to such experiment is insecure.The chemistry neutralisation is the sterilizing agent method of at present the most frequently used removal remnants, and its advantage is that method is simple, and is easy to use, reliable for effect.The biological enzyme neutralizing agent can be used for estimating the disinfection of biological enzyme thimerosal.Existing thimerosal neutralizing agent kind is a lot, and common have acid, alkali, absorption, dilution, centrifugal a, filtration etc.The above-mentioned screening of all passing through strictness also can't not determined its working concentration; Corresponding sterilizing agent do not had solid neutralizing effect; Neutralizing agent itself or with the product of sterilizing agent reaction the experiment microorganism is had killing or suppress the effect of its growth; To cultivating composition destruction is arranged, also influence its physical behavior.Along with the sterilization progress of research, both at home and abroad to the research of neutralizing agent also in continuous development.The disinfection person wishes to have always and a kind ofly can overcome the insufficient neutralizing agent of above-mentioned neutralizing agent.
Background technology the object of the present invention is to provide and a kind ofly can overcome the insufficient compound neutralizing agent of existing sterilization neutralizing agent---biological enzyme neutralizing agent, compares with other class neutralizing agent to possess following characteristics: corresponding biological enzyme sterilizing agent is had solid neutralizing effect; Neutralizing agent itself or with the product of biological enzyme sterilizing agent reaction the experiment microorganism is not had killing or suppress the effect of its growth; Do not have destruction to cultivating composition, also do not influence its physical behavior.
Summary of the invention main points of the present invention are to select suitable component, and rationally are mixed, through heat, dissolve, technology such as mixing, cooling, packing, high-temperature sterilization forms a kind of biological enzyme thimerosal neutralizing agent.
The prescription following (component and weight percent % thereof) that the present invention selects: Sulfothiorine (0.1-0.5), Yelkin TTS (0.1-0.3), soil temperature-80 (0.1-0.3), soap base (0.1-0.3), nutrient broth (20-30), purifying distilled water add to 100.
Sulfothiorine, Yelkin TTS, soil temperature-80, soap base, meat soup can with the biological enzyme thimerosal generation chemical reaction of remnants, generate to the experiment microorganism do not kill, suppress its growth effect, do not have destruction to cultivating composition, physical behavior not have the neutralized reaction product that influences.Corresponding biological enzyme sterilizing agent had solid neutralizing effect.
The preparation method of product of the present invention is:
1, nutrient broth is heated, is dissolved configuration in proportion;
2, soap base is carried out in proportion, heats dissolving;
3, Sulfothiorine, soil temperature-80, Yelkin TTS are dissolved in proportion 54 ℃ of the pure middle heating of the two aquae destillatas of purifying, cooling;
4, dissolved soap base, nutrient broth are joined by proportioning in the mixing solutions such as cooled Sulfothiorine, remix evenly, carry out packing after the cooling, 105 ℃ of sterilizations 15 minutes.Steriling test, warehousing after passing.
The present invention has the following advantages: corresponding biological enzyme sterilizing agent is had solid neutralizing effect; The product of neutralizing agent itself and the reaction of biological enzyme sterilizing agent is to testing the effect that microorganism does not have killing, suppresses its growth; Do not have destruction to cultivating composition, also do not influence its physical behavior, it is clear to count.
The present invention is described in further detail below in conjunction with embodiment for embodiment:
Be configured according to following table institute column data (weight percent %) and described step thereof:
Prescription one:
Sulfothiorine 0.1-0.5
Yelkin TTS 0.1-0.3
Soil temperature-80 0.1-0.3
Soap base 0.1-0.3
Nutrient broth 20.0-30.0
The two aquae destillatas of purifying add to 100
Prescription two:
Sulfothiorine 0.1-0.3
Yelkin TTS 0.1-0.2
Soil temperature-80 0.1-0.2
Soap base 0.1-0.2
Nutrient broth 20.0-25.0
The two aquae destillatas of purifying add to 100
Prescription three:
Sulfothiorine 0.2-0.5
Yelkin TTS 0.2-0.3
Soil temperature-80 0.2-0.3
Soap base 0.2-0.3
Nutrient broth 22.0-30.0
The two aquae destillatas of purifying add to 100
Prescription four:
Sulfothiorine 0.1-0.4
Yelkin TTS 0.2-0.3
Soil temperature-80 0.1-0.2
Soap base 0.1-0.3
Nutrient broth 20.0-30.0
The two aquae destillatas of purifying add to 100
Claims (2)
1, a kind of biological enzyme neutralizing agent of the present invention, a kind of biological enzyme neutralizing agent, its main component is that Sulfothiorine, Yelkin TTS, soil temperature-80, soap base, nutrient broth are mixed in proportion; It is characterized in that having following prescription (component and weight percent % thereof): Sulfothiorine (0.1-0.5); Yelkin TTS (0.1-0.3); Soil temperature-80 (0.1-0.3); Soap base (0.1-0.3); Nutrient broth (20-30); Purified water adds to 100.
2, the preparation method of the described biological enzyme neutralizing agent of a kind of claim 1 is characterized in that having following step:
1), nutrient broth is heated, is dissolved configuration in proportion;
2), soap base is carried out in proportion, heats dissolving;
3), Sulfothiorine, soil temperature-80, Yelkin TTS are dissolved in 54 ℃ of the pure middle heating of the two aquae destillatas of purifying, cooling in proportion;
4), dissolved soap base, nutrient broth joined by proportioning in the mixing solutions such as cooled Sulfothiorine, remix evenly, carry out packing after the cooling, 105 ℃ of sterilizations 15 minutes, steriling test, warehousing after passing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101157611A CN101463377A (en) | 2007-12-18 | 2007-12-18 | Biological enzyme neutralization agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101157611A CN101463377A (en) | 2007-12-18 | 2007-12-18 | Biological enzyme neutralization agent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101463377A true CN101463377A (en) | 2009-06-24 |
Family
ID=40804167
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007101157611A Withdrawn CN101463377A (en) | 2007-12-18 | 2007-12-18 | Biological enzyme neutralization agent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101463377A (en) |
-
2007
- 2007-12-18 CN CNA2007101157611A patent/CN101463377A/en not_active Withdrawn
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| Date | Code | Title | Description |
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| C57 | Notification of unclear or unknown address | ||
| DD01 | Delivery of document by public notice |
Addressee: Zhang Hongtao Document name: Notification of Passing Preliminary Examination of the Application for Invention |
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| C04 | Withdrawal of patent application after publication (patent law 2001) | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| WW01 | Invention patent application withdrawn after publication |