CN101698868A - Cysteine neutralizing and sampling liquid of plant disinfectant - Google Patents
Cysteine neutralizing and sampling liquid of plant disinfectant Download PDFInfo
- Publication number
- CN101698868A CN101698868A CN200710115728A CN200710115728A CN101698868A CN 101698868 A CN101698868 A CN 101698868A CN 200710115728 A CN200710115728 A CN 200710115728A CN 200710115728 A CN200710115728 A CN 200710115728A CN 101698868 A CN101698868 A CN 101698868A
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- Prior art keywords
- plant disinfectant
- tween
- halfcystine
- disinfectant
- cysteine
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Abstract
The invention relates to cysteine neutralizing and sampling liquid of a plant disinfectant, which is an agent prepared by mixing main ingredients including cysteine, tween-80, lecithin and glycin in a ratio, wherein the cysteine, the tween-80, the lecithin and the glycin can chemically react with the residual plant disinfectant to form neutral products that avoid producing killing or growth-inhibiting effects on experimental microorganisms, damages to culture ingredients and influences on physical properties, but have exact and reliable neutralization effect on the corresponding plant disinfectant. The liquid is suitable to be used for disinfection effect of the plant disinfectant. The invention the advantages that: the liquid has exact and reliable neutralization effect on the plant disinfectant; and the neutralizing agent or the products of the reaction of the neutralizing agent and the plant disinfectant avoid killing or inhibiting the growth of the experimental organisms, causing damages to culture ingredients and influencing the physical properties of the experimental organisms.
Description
Technical field the present invention relates in a kind of halfcystine plant disinfectant and sample solution, and its main component is halfcystine, tween-80, Yelkin TTS, glycine, is mixed in proportion into mixture.The key problem of disinfection experiment is to estimate the sterilization and the bacteriostatic action of disinfection factor.Estimate the effect of plant disinfectant, must strictly grasp action time.Plant disinfectant and microorganism touch after the preset time, must remove its effect immediately, so just can accurately obtain the data of plant disinfectant action effect; Simultaneously must not contain remaining plant disinfectant in the sample after taking to sterilize.To prevent that in recover cultivating microorganism from continuing to be subjected to the effect of plant disinfectant.Therefore, the experiment that any one estimates plant disinfectant all must have the reliable method of removing remaining plant disinfectant.Do not adopt the experiment of removing remaining thimerosal measure, should think serious mistake in design, the result who draws according to such experiment is insecure.The chemistry neutralisation is the plant disinfectant method of at present the most frequently used removal remnants, and its advantage is that method is simple, and is easy to use, reliable for effect.Can be used for estimating the disinfection of plant disinfectant in the halfcystine plant disinfectant with sample solution.Existing thimerosal neutralizing agent kind is a lot, and common have acid, alkali, absorption, dilution, centrifugal a, filtration etc.The above-mentioned screening of all passing through strictness also can't not determined its working concentration; Corresponding plant disinfectant do not had solid neutralizing effect; Neutralizing agent itself or with the product of thimerosal reaction the experiment microorganism is had killing or suppress the effect of its growth; To cultivating composition destruction is arranged, also influence its physical behavior.Along with the sterilization progress of research, both at home and abroad to the research of neutralizing agent also in continuous development.The disinfection person wishes to have always and a kind ofly can overcome the insufficient neutralizing agent of above-mentioned neutralizing agent.
Background technology the object of the present invention is to provide and a kind ofly can overcome in the insufficient halfcystine plant disinfectant of existing sterilization neutralizing agent and sample solution, compares with other class neutralizing agent to possess following characteristics: corresponding plant disinfectant is had solid neutralizing effect; Neutralizing agent itself or with the product of plant disinfectant reaction the experiment microorganism is not had killing or suppress the effect of its growth; Do not have destruction to cultivating composition, also do not influence its physical behavior.
Summary of the invention main points of the present invention are to select suitable component, and rationally are mixed, through heat, dissolve, technology such as mixing, cooling, packing, high-temperature sterilization forms in a kind of halfcystine plant disinfectant and sample solution.
The prescription following (component and weight percent % thereof) that the present invention selects: halfcystine (0.1-0.3); Tween-80 (1.0-3.0); Yelkin TTS (0.1-0.3); Glycine (0.1-0.3); Purified water adds to 100.
Halfcystine, tween-80, Yelkin TTS, glycine, can with the plant disinfectant generation chemical reaction of remnants, generate to the experiment microorganism do not kill or suppress its growth effect, do not have destruction to cultivating composition, physical behavior not have the neutralized reaction product that influences.Corresponding plant disinfectant had solid neutralizing effect.
The preparation method of product of the present invention is:
1), tween-80, Yelkin TTS are heated in proportion, dissolved.
2), glycine, halfcystine are dissolved in the pure water 54 ℃ of heating in proportion, cooling.
3), dissolved tween-80, Yelkin TTS are joined in cooling back glycine, the halfcystine solution by proportioning, mix, carry out packing after the cooling, 105 ℃ of sterilizations 15 minutes.Check, warehousing after passing.
The present invention has the following advantages: corresponding plant disinfectant is had solid neutralizing effect; Neutralizing agent itself or with the product of plant disinfectant reaction the experiment microorganism is not had killing or suppress the effect of its growth; Do not have destruction to cultivating composition, also do not influence its physical behavior.
The present invention is described in further detail below in conjunction with embodiment for embodiment:
Be configured according to following table institute column data (weight percent %) and described step thereof:
Claims (2)
1. the present invention relates in a kind of halfcystine plant disinfectant and sample solution, its main component is halfcystine, tween-80, Yelkin TTS, glycine, is mixed in proportion into mixture; It is characterized in that having following prescription (component and weight percent % thereof): halfcystine (0.1-0.3); Tween-80 (1.0-3.0); Yelkin TTS (0.1-0.3); Glycine (0.1-0.3); Purified water adds to 100.
2. the preparation method of the described plant disinfectant neutralizing agent of claim 1 is characterized in that having following step:
1), tween-80, Yelkin TTS are heated in proportion, dissolved;
2), glycine, halfcystine are dissolved in the pure water 54 ℃ of heating in proportion, cooling;
3), dissolved tween-80, Yelkin TTS are joined in cooling back glycine, the halfcystine solution by proportioning, mix, carry out packing after the cooling, 105 ℃ of sterilizations 15 minutes, check, warehousing after passing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710115728A CN101698868A (en) | 2007-12-18 | 2007-12-18 | Cysteine neutralizing and sampling liquid of plant disinfectant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710115728A CN101698868A (en) | 2007-12-18 | 2007-12-18 | Cysteine neutralizing and sampling liquid of plant disinfectant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101698868A true CN101698868A (en) | 2010-04-28 |
Family
ID=42147333
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200710115728A Pending CN101698868A (en) | 2007-12-18 | 2007-12-18 | Cysteine neutralizing and sampling liquid of plant disinfectant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101698868A (en) |
-
2007
- 2007-12-18 CN CN200710115728A patent/CN101698868A/en active Pending
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20100428 |