CN101698864A - Air sampling neutralization culture medium in public sanitary place - Google Patents
Air sampling neutralization culture medium in public sanitary place Download PDFInfo
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- CN101698864A CN101698864A CN200710115724A CN200710115724A CN101698864A CN 101698864 A CN101698864 A CN 101698864A CN 200710115724 A CN200710115724 A CN 200710115724A CN 200710115724 A CN200710115724 A CN 200710115724A CN 101698864 A CN101698864 A CN 101698864A
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- sanitary place
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Abstract
The invention relates to an air sampling neutralization culture medium in a public sanitary place, which comprises the following main components: soil temperature-80, lecithin, sodium thiosulfate, glucose, tryptone and agar which are mixed in a ratio. The air sampling neutralization culture medium in the public sanitary place can be used for evaluating cultivation of a bacterium solid culture medium after disinfection of an air disinfectant in the public sanitary place. The lecithin, Tween-80 and the sodium thiosulfate can generate chemical reaction with the residual air disinfectant in the public sanitary place to generate a neutralization product which has no effects of killing experimental microorganisms and inhibiting growth of the experimental microorganisms, or destructive effect on cultivated components or influence on physical properties. The culture medium has practical and reliable neutralization for the corresponding compound air disinfectant in the public sanitary place. The glucose, the tryptone and the agar are nutritive substrates in a glucose and agar culture medium, experimental bacteria are enriched and bred in the solid neutralization culture medium, and the residual air disinfectant in the public sanitary place is correspondingly neutralized. The culture medium has the advantages that: (1) the culture medium is the solid culture medium, and has practical and reliable neutralization for the corresponding air disinfectant in the public sanitary place; (2) a reaction product of the air sampling neutralization culture medium in the public sanitary place and the air disinfectant in the public sanitary place has no effects of killing the experimental microorganisms and inhibiting growth of the experimental microorganisms; (3) the culture medium has no destructive effect on the cultivated components or influence on the physical properties; and (4) the experimental bacteria are enriched and bred in the solid culture medium.
Description
Technical field the present invention relates in a kind of public sanitary place air sampling and substratum, and its main component is that soil temperature-80, Yelkin TTS, Sulfothiorine, glucose, Tryptones, agar are mixed in proportion and form.The key problem of disinfection experiment is to estimate the sterilization and the bacteriostatic action of disinfection factor.Estimate the effect of public sanitary place air, must strictly grasp action time.Public sanitary place air sanitizer and microorganism touch after the preset time, must remove its effect immediately, so just can accurately obtain the data of public sanitary place air sanitizer action effect; Simultaneously must not contain remaining public sanitary place air sanitizer in the sample after taking to sterilize.To prevent that in recover cultivating microorganism from continuing to be subjected to the effect of public sanitary place air sanitizer.Therefore, do not adopt the experiment of removing remaining public sanitary place air sanitizer measure, should think serious mistake in design, the result who draws according to such experiment is insecure.The chemistry neutralisation is the public sanitary place sterilizing air agent method of at present the most frequently used removal remnants, and its advantage is that method is simple, and is easy to use, reliable for effect.In the public sanitary place air sampling and the substratum bacterium solid culture medium that can be used for estimating after the disinfection of public sanitary place air sanitizer cultivate.Existing thimerosal neutralizing agent kind is a lot, and common have acid, alkali, absorption, dilution, centrifugal a, filtration etc.The above-mentioned screening of all passing through strictness also can't not determined its working concentration; Corresponding public sanitary place air sanitizer do not had solid neutralizing effect; Neutralizing agent itself or the experiment microbial bacterial is cultivated the effect that killing is arranged or suppress its growth with the product of public sanitary place air sanitizer reaction; To cultivating composition destruction is arranged, also influence its physical behavior.Along with the sterilization progress of research, both at home and abroad to the research of neutralizing agent also in continuous development.The disinfection person wishes to have always and a kind ofly can overcome in the air sampling of the insufficient neutralizing agent of above-mentioned neutralizing agent----public sanitary place and substratum.
Background technology the object of the present invention is to provide and a kind ofly can overcome in the insufficient public sanitary place air sampling of existing sterilization neutralizing agent and substratum.Compare with other class neutralizing agent and to possess following characteristics: corresponding public sanitary place air sanitizer is had solid neutralizing effect; The product that reacts with substratum itself and public sanitary place air sanitizer in the public sanitary place air sampling is to testing the effect that microorganism does not have killing or suppresses its growth; Do not have destruction to cultivating composition, also do not influence its physical behavior; The experiment bacterium not only increases the bacterium breeding in this solid medium, and residual public sanitary place air sanitizer obtains corresponding neutralization.
Summary of the invention main points of the present invention are to select suitable component, and rationally are mixed, through heat, dissolve, technology such as mixing, cooling, packing, high-temperature sterilization forms in a kind of public sanitary place air sampling and substratum.
The prescription following (component and weight percent % thereof) that the present invention selects: Yelkin TTS (0.1-0.3); Soil temperature-80 (0.5-1.0); Sulfothiorine (0.1-1.0); Glucose (1.5-2.0); Tryptones (1.0-1.5); Agar (2.0-2.5); Distilled water adds to 100.
Yelkin TTS, soil temperature-80, Sulfothiorine can with the public sanitary place air sanitizer generation chemical reaction of remnants, generate to the experiment microorganism do not kill or suppress its growth effect, do not have destruction to cultivating composition, physical behavior not have the neutralized reaction product that influences.Corresponding compound public sanitary place air sanitizer had solid neutralizing effect.Glucose, Tryptones, agar are the nutraceutical matrix in the glucose agar medium, and the experiment bacterium not only increases the bacterium breeding in this solid medium, and residual public sanitary place air sanitizer obtains corresponding neutralization.
The preparation method of product of the present invention is:
1), polysorbate-80, Sulfothiorine, Yelkin TTS are heated in proportion, dissolved.
2), with glucose, Tryptones, agar in proportion heating for dissolving in distilled water, be chilled to 54 ℃.
3), with dissolved 2) mixed solution joins dissolved 2) in the solution, mix, the cooling back transfers PH6.1-6.3.
4), after the packing, 115 ℃ of pressuresteam sterilizations, 20-30min.
The present invention has the following advantages: (1) is in the solid and substratum, and corresponding public sanitary place air sanitizer is had solid neutralizing effect; (2) product that reacts with substratum itself and public sanitary place air sanitizer in the public sanitary place air sampling is to testing the effect that microorganism does not have killing or suppresses its growth; (3) do not have destruction to cultivating composition, also do not influence its physical behavior; (4) the experiment bacterium not only increases the bacterium breeding in this solid medium, and residual public sanitary place air sanitizer obtains corresponding neutralization.
The present invention is described in further detail below in conjunction with embodiment for embodiment:
Be configured according to following table institute column data (weight percent %) and described step thereof:
Claims (2)
1. the present invention relates in a kind of public sanitary place air sampling and substratum, its main component is that soil temperature-80, Yelkin TTS, Sulfothiorine, glucose, Tryptones, agar are mixed in proportion and form.It is characterized in that having following prescription (component and weight percent % thereof): Yelkin TTS (0.1-0.3); Soil temperature-80 (0.5-1.0); Sulfothiorine (0.1-1.0); Glucose (1.5-2.0); Tryptones (1.0-1.5); Agar (2.0-2.5); Distilled water adds to 100.
2. in the described public sanitary place air sampling of claim 1 and the preparation method of substratum, it is characterized in that having following step:
1), polysorbate-80, Sulfothiorine, Yelkin TTS are heated in proportion, dissolved;
2), with glucose, Tryptones, agar in proportion heating for dissolving in distilled water, be chilled to 54 ℃;
3), with dissolved 1) mixed solution joins dissolved 2) in the solution, mix, the cooling back transfers PH6.1-6.3;
4), after the packing, 115 ℃ of pressuresteam sterilizations, 20-30min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710115724A CN101698864A (en) | 2007-12-18 | 2007-12-18 | Air sampling neutralization culture medium in public sanitary place |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710115724A CN101698864A (en) | 2007-12-18 | 2007-12-18 | Air sampling neutralization culture medium in public sanitary place |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101698864A true CN101698864A (en) | 2010-04-28 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200710115724A Pending CN101698864A (en) | 2007-12-18 | 2007-12-18 | Air sampling neutralization culture medium in public sanitary place |
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| CN (1) | CN101698864A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106350575A (en) * | 2016-08-31 | 2017-01-25 | 山东新华医疗器械股份有限公司 | Embossing method for bacterium sampling |
| CN107151688A (en) * | 2017-05-23 | 2017-09-12 | 新乡医学院 | In a kind of public sanitary place air sampling and culture medium |
-
2007
- 2007-12-18 CN CN200710115724A patent/CN101698864A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106350575A (en) * | 2016-08-31 | 2017-01-25 | 山东新华医疗器械股份有限公司 | Embossing method for bacterium sampling |
| CN107151688A (en) * | 2017-05-23 | 2017-09-12 | 新乡医学院 | In a kind of public sanitary place air sampling and culture medium |
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Open date: 20100428 |