CN101029324A - Neutralizing glucose agar medium - Google Patents
Neutralizing glucose agar medium Download PDFInfo
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- CN101029324A CN101029324A CN 200610042441 CN200610042441A CN101029324A CN 101029324 A CN101029324 A CN 101029324A CN 200610042441 CN200610042441 CN 200610042441 CN 200610042441 A CN200610042441 A CN 200610042441A CN 101029324 A CN101029324 A CN 101029324A
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- glucose
- agar
- neutralizing
- agar medium
- tryptones
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Abstract
A neutralized glucose agar culture medium consists of Tween-80, lecithin, sodium hyposulfite, glucose, trypsatone and agar. The process is carried out by mixing proportionally, chemical reacting lecithin, Tween-80 and sodium hyposulfite with compound disinfectant and neutralizing. It has better neutralizing function, no inhibition for microbe and destructive role for nutrients. It can be used for fungus solid culture medium.
Description
Technical field the present invention relates to a kind of in and glucose agar medium, its main component is that soil temperature-80, Yelkin TTS, Sulfothiorine, glucose, Tryptones, agar are mixed in proportion and form.The key problem of disinfection experiment is to estimate the sterilization and the bacteriostatic action of disinfection factor.The effect of evaluating chemical sterilizing agent must strictly be grasped action time.Sterilizing agent and microorganism touch after the preset time, must remove its effect immediately, so just can accurately obtain the data of sterilizing agent action effect; Simultaneously must not contain remaining sterilizing agent in the sample after taking to sterilize.To prevent that in recover cultivating microorganism from continuing to be subjected to the effect of sterilizing agent.Therefore, do not adopt the experiment of removing remaining sterilizing agent measure, should think serious mistake in design, the result who draws according to such experiment is insecure.The chemistry neutralisation is the sterilizing agent method of at present the most frequently used removal remnants, and its advantage is that method is simple, and is easy to use, reliable for effect.In and glucose agar medium can be used for estimating fungus solids culture medium culturing after the compound disinfectant disinfection.Existing thimerosal neutralizing agent kind is a lot, and common have acid, alkali, absorption, dilution, centrifugal a, filtration etc.The above-mentioned screening of all passing through strictness also can't not determined its working concentration; Corresponding sterilizing agent do not had solid neutralizing effect; Neutralizing agent itself or the experiment microbial bacterial is cultivated the effect that killing is arranged or suppress its growth with the product of sterilizing agent reaction; To cultivating composition destruction is arranged, also influence its physical behavior.Along with the sterilization progress of research, both at home and abroad to the research of neutralizing agent also in continuous development.The disinfection person wishes to have always and a kind ofly can overcome in the insufficient neutralizing agent of above-mentioned neutralizing agent----and glucose agar medium.
Background technology the object of the present invention is to provide a kind of can overcome existing sterilization neutralizing agent insufficient in and glucose agar medium.Compare with other class neutralizing agent and to possess following characteristics: corresponding compound disinfectant is had solid neutralizing effect; In and the product of glucose agar medium itself and sterilizing agent reaction, to testing the effect that microorganism does not have killing or suppresses its growth; Do not have destruction to cultivating composition, also do not influence its physical behavior; The experiment fungi not only increases the bacterium breeding in this solid medium, and the residual disinfectancy agent obtains corresponding neutralization.
Summary of the invention main points of the present invention are to select suitable component, and rationally are mixed, through heat, dissolve, technology such as mixing, cooling, packing, high-temperature sterilization form a kind of in and glucose agar medium.
The prescription following (component and weight percent % thereof) that the present invention selects: Yelkin TTS (0.1-0.3); Soil temperature-80 (0.5-1.0); Sulfothiorine (0.1-1.0); Glucose (1.5-2.0); Tryptones (1.0-1.5); Agar (2.0-2.5); Distilled water adds to 100.
Yelkin TTS, soil temperature-80, Sulfothiorine can with the compound disinfectant generation chemical reaction of remnants, generate to the experiment microorganism do not kill or suppress its growth effect, do not have destruction to cultivating composition, physical behavior not have the neutralized reaction product that influences.Corresponding compound disinfectant had solid neutralizing effect.Glucose, Tryptones, agar are the nutraceutical matrix in the glucose agar medium, and the experiment fungi not only increases the bacterium breeding in this solid medium, and the residual disinfectancy agent obtains corresponding neutralization.
The preparation method of product of the present invention is:
1), polysorbate-80, Sulfothiorine, Yelkin TTS are heated in proportion, dissolved.
2), with glucose, Tryptones, agar in proportion heating for dissolving in distilled water, be chilled to 54 ℃.
3), with dissolved 2) mixed solution joins dissolved 2) in the solution, mix, the cooling back transfers PH6.1-6.3.
4), after the packing, 115 ℃ of pressuresteam sterilizations, 20-30min.
The present invention has the following advantages: (1) is in the solid and substratum, and corresponding compound disinfectant is had solid neutralizing effect; (2) product that reacts with glucose agar medium itself and sterilizing agent in is to testing the effect that microorganism does not have killing or suppresses its growth; (3) do not have destruction to cultivating composition, also do not influence its physical behavior; (4) the experiment fungi not only increases the bacterium breeding in this solid medium, and the residual disinfectancy agent obtains corresponding neutralization.
The present invention is described in further detail below in conjunction with embodiment for embodiment:
Be configured according to following table institute column data (weight percent %) and described step thereof:
Prescription one:
Yelkin TTS 0.1-0.3
Polysorbate-80 0.5-1.0
Sulfothiorine 0.1-1.0
Glucose 1.5-2.0
Tryptones 1.0-1.5
Agar 2.0-2.5
Distilled water adds to 100
Prescription two:
Yelkin TTS 0.2-0.3
Polysorbate-80 0.6-1.0
Sulfothiorine 0.5-1.0
Glucose 1.6-2.0
Tryptones 1.3-1.5
Agar 2.2-2.5
Distilled water adds to 100
Prescription three:
Yelkin TTS 0.1-0.2
Polysorbate-80 0.5-0.8
Sulfothiorine 0.1-0.7
Glucose 1.5-1.8
Tryptones 1.0-1.3
Agar 2.0-2.4
Distilled water adds to 100
Prescription four:
Yelkin TTS 0.2-0.3
Polysorbate-80 0.6-0.8
Sulfothiorine 0.5-0.6
Glucose 1.6-1.8
Tryptones 1.3-1.4
Agar 2.2-2.4
Distilled water adds to 100
Claims (2)
1, a kind of with soil temperature-80, Yelkin TTS, Sulfothiorine, glucose, Tryptones, agar be main ingredient in and glucose agar medium.It is characterized in that having following prescription (component and weight percent % thereof): Yelkin TTS (0.1-0.3); Soil temperature-80 (0.5-1.0); Sulfothiorine (0.1-1.0); Glucose (1.5-2.0); Tryptones (1.0-1.5); Agar (2.0-2.5); Distilled water adds to 100.
2, a kind of claim 1 described in and the preparation method of glucose agar medium, it is characterized in that having following step:
1), polysorbate-80, Sulfothiorine, Yelkin TTS are heated in proportion, dissolved.
2), with glucose, Tryptones, agar in proportion heating for dissolving in distilled water, be chilled to 54 ℃.
3), with dissolved 2) mixed solution joins dissolved 2) in the solution, mix, the cooling back transfers PH6.1-6.3.
4), after the packing, 115 ℃ of pressuresteam sterilizations, 20-30min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610042441 CN101029324A (en) | 2006-02-27 | 2006-02-27 | Neutralizing glucose agar medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610042441 CN101029324A (en) | 2006-02-27 | 2006-02-27 | Neutralizing glucose agar medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101029324A true CN101029324A (en) | 2007-09-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610042441 Pending CN101029324A (en) | 2006-02-27 | 2006-02-27 | Neutralizing glucose agar medium |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107151688A (en) * | 2017-05-23 | 2017-09-12 | 新乡医学院 | In a kind of public sanitary place air sampling and culture medium |
-
2006
- 2006-02-27 CN CN 200610042441 patent/CN101029324A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107151688A (en) * | 2017-05-23 | 2017-09-12 | 新乡医学院 | In a kind of public sanitary place air sampling and culture medium |
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