CN101565727A - Complex neutralizer for disinfectant - Google Patents
Complex neutralizer for disinfectant Download PDFInfo
- Publication number
- CN101565727A CN101565727A CNA2008100934218A CN200810093421A CN101565727A CN 101565727 A CN101565727 A CN 101565727A CN A2008100934218 A CNA2008100934218 A CN A2008100934218A CN 200810093421 A CN200810093421 A CN 200810093421A CN 101565727 A CN101565727 A CN 101565727A
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- China
- Prior art keywords
- neutralizing agent
- agent
- sulfothiorine
- sterilizing agent
- soap base
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Abstract
The invention relates to a complex neutralizing agent for a disinfecting agent. The complex neutralizing agent is formed by special process by proportionally mixing the components of sodium hyposulfite, lecithin, tween-80, soap base and nutrient broth and is prepared by a special process through mixing the components according to a proportion. The complex neutralizing agent is applied to the disinfection effect of disinfectant products such as biological enzymes, carbamidines, and the like, all of which are used for evaluating disinfection experiments. The invention has the advantages that the corresponding disinfectant products such as the biological enzymes, the carbamidines, and the like has practical and reliable neutralizing effect; the products of reaction of the complex neutralizing agent and the disinfectant products such as the biological enzymes, the carbamidines, and the like do not kill experimental microorganism but inhibit the growth of the experimental microorganism; and the complex neutralizing agent causes no damage to culture ingredients and does not influence the physical properties of the culture ingredients.
Description
Technical field
The present invention relates to the compound neutralizing agent of a kind of sterilizing agent, its main component is that Sulfothiorine, Yelkin TTS, soil temperature-80, soap base, nutrient broth are mixed in proportion into mixture.The key problem of disinfection experiment is to estimate sterilization, antibacterial, the anti-microbial effect of disinfection factor.Estimate the effect of sterilizing agent, must strictly grasp action time.Sterilizing agent and microorganism touch after the preset time, must remove its effect immediately, so just can accurately obtain the data of sterilizing agent action effect; Simultaneously must not contain remaining sterilizing agent in the sample after taking to sterilize.To prevent that in recover cultivating microorganism from continuing to be subjected to the effect of sterilizing agent.Therefore, the experiment of any one evaluating chemical sterilizing agent all must have the reliable method of removing remaining sterilizing agent.Do not adopt the experiment of removing remaining sterilizing agent measure, should think serious mistake in design, the result who draws according to such experiment is insecure.The chemistry neutralisation is the sterilizing agent method of at present the most frequently used removal remnants, and its advantage is that method is simple, and is easy to use, reliable for effect.The compound neutralizing agent of sterilizing agent can be used for estimating the disinfection of biological enzyme, guanidine disinfectant.Existing thimerosal neutralizing agent kind is a lot, and common have acid, alkali, absorption, dilution, centrifugal a, filtration etc.The above-mentioned screening of all passing through strictness also can't not determined its working concentration; Corresponding sterilizing agent do not had solid neutralizing effect; Neutralizing agent itself or with the product of sterilizing agent reaction the experiment microorganism is had killing or suppress the effect of its growth; To cultivating composition destruction is arranged, also influence its physical behavior.Along with the sterilization progress of research, both at home and abroad to the research of neutralizing agent also in continuous development.The disinfection person wishes to have always and a kind ofly can overcome the insufficient neutralizing agent of above-mentioned neutralizing agent.
Background technology
The object of the present invention is to provide a kind ofly can overcome the insufficient compound neutralizing agent of existing sterilization neutralizing agent---the compound neutralizing agent of sterilizing agent, compare with other class neutralizing agent and possess following characteristics: corresponding biological enzyme, guanidine class sterilizing agent are had solid neutralizing effect; Neutralizing agent itself or with the product of biological enzyme, guanidine class sterilizing agent reaction the experiment microorganism is not had killing or suppress the effect of its growth; Do not have destruction to cultivating composition, also do not influence its physical behavior.
Summary of the invention
Main points of the present invention are to select suitable component, and rationally are mixed, through heat, dissolve, technology such as mixing, cooling, packing, high-temperature sterilization forms a kind of biological enzyme, guanidine disinfectant neutralizing agent.
The prescription following (component and weight percent % thereof) that the present invention selects: Sulfothiorine (0.1-0.5), Yelkin TTS (0.1-0.3), soil temperature-80 (0.1-0.3), soap base (0.1-0.3), nutrient broth (20-30), purifying distilled water add to 100.
Sulfothiorine, Yelkin TTS, soil temperature-80, soap base, meat soup can with biological enzyme, the guanidine disinfectant generation chemical reaction of remnants, generate to the experiment microorganism do not kill, suppress its growth effect, do not have destruction to cultivating composition, physical behavior not have the neutralized reaction product that influences.Corresponding biological enzyme, guanidine class sterilizing agent had solid neutralizing effect.
The preparation method of product of the present invention is:
1, nutrient broth is heated, is dissolved configuration in proportion;
2, soap base is carried out in proportion, heats dissolving;
3, Sulfothiorine, soil temperature-80, Yelkin TTS are dissolved in proportion 54 ℃ of the pure middle heating of the two aquae destillatas of purifying, cooling;
3, dissolved soap base, nutrient broth are joined by proportioning in the mixing solutions such as cooled Sulfothiorine, remix evenly, carry out packing after the cooling, 105 ℃ of sterilizations 15 minutes.Steriling test, warehousing after passing.
The present invention has the following advantages: corresponding biological enzyme, guanidine class sterilizing agent are had solid neutralizing effect; The product of neutralizing agent itself and biological enzyme, the reaction of guanidine class sterilizing agent is to testing the effect that microorganism does not have killing, suppresses its growth; Do not have destruction to cultivating composition, also do not influence its physical behavior, it is clear to count.
Embodiment
The present invention is described in further detail below in conjunction with embodiment:
Be configured according to following table institute column data (weight percent %) and described step thereof:
Prescription one:
Sulfothiorine 0.1-0.5
Yelkin TTS 0.1-0.3
Soil temperature-80 0.1-0.3
Soap base 0.1-0.3
Nutrient broth 20.0-30.0
The two aquae destillatas of purifying add to 100
Prescription two:
Sulfothiorine 0.1-0.3
Yelkin TTS 0.1-0.2
Soil temperature-80 0.1-0.2
Soap base 0.1-0.2
Nutrient broth 20.0-25.0
The two aquae destillatas of purifying add to 100
Prescription three:
Sulfothiorine 0.2-0.5
Yelkin TTS 0.2-0.3
Soil temperature-80 0.2-0.3
Soap base 0.2-0.3
Nutrient broth 22.0-30.0
The two aquae destillatas of purifying add to 100
Prescription four:
Sulfothiorine 0.1-0.4
Yelkin TTS 0.2-0.3
Soil temperature-80 0.1-0.2
Soap base 0.1-0.3
Nutrient broth 20.0-30.0
The two aquae destillatas of purifying add to 100
Claims (2)
1, the compound neutralizing agent of a kind of sterilizing agent of the present invention is sterilization, antibacterial, the anti-microbial effect that is used for disinfection experiment, estimates biological enzyme, guanidine class sterilizing agent disinfection factor; It is characterized in that having following prescription (component and weight percent % thereof): Sulfothiorine (0.1-0.5); Yelkin TTS (0.1-0.3); Soil temperature-80 (0.1-0.3); Soap base (0.1-0.3); Nutrient broth (20-30); Purified water adds to 100.
2, the preparation method of the compound neutralizing agent of the described sterilizing agent of a kind of claim 1 is characterized in that having following step:
1), nutrient broth is heated, is dissolved configuration in proportion;
2), soap base is carried out in proportion, heats dissolving;
3), Sulfothiorine, soil temperature-80, Yelkin TTS are dissolved in 54 ℃ of the pure middle heating of the two aquae destillatas of purifying, cooling in proportion;
4), dissolved soap base, nutrient broth joined by proportioning in the mixing solutions such as cooled Sulfothiorine, remix evenly, carry out packing after the cooling, 105 ℃ of sterilizations 15 minutes, steriling test, warehousing after passing.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNA2008100934218A CN101565727A (en) | 2008-04-21 | 2008-04-21 | Complex neutralizer for disinfectant |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNA2008100934218A CN101565727A (en) | 2008-04-21 | 2008-04-21 | Complex neutralizer for disinfectant |
Publications (1)
Publication Number | Publication Date |
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CN101565727A true CN101565727A (en) | 2009-10-28 |
Family
ID=41282091
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CNA2008100934218A Pending CN101565727A (en) | 2008-04-21 | 2008-04-21 | Complex neutralizer for disinfectant |
Country Status (1)
Country | Link |
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CN (1) | CN101565727A (en) |
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2008
- 2008-04-21 CN CNA2008100934218A patent/CN101565727A/en active Pending
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Open date: 20091028 |