CN101698877A - Sampling solution for neutralizing marine organism disinfecting solution - Google Patents
Sampling solution for neutralizing marine organism disinfecting solution Download PDFInfo
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- CN101698877A CN101698877A CN200710115721A CN200710115721A CN101698877A CN 101698877 A CN101698877 A CN 101698877A CN 200710115721 A CN200710115721 A CN 200710115721A CN 200710115721 A CN200710115721 A CN 200710115721A CN 101698877 A CN101698877 A CN 101698877A
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Abstract
The invention relates to a sampling solution for neutralizing a marine organism disinfecting solution, which is prepared into a mixture by mixing the main components of polysorbate-80, lecithin and agar in proportions. The key problem of the disinfecting experiment is the evaluation of the bactericidal and bacteriostatic actions of disinfecting factors. The polysorbate-80, the lecithin and the agar can perform chemical reaction with the residual marine organism disinfecting solution to generate a neutralized product which has no killing effect on the experimental micro-organisms or no inhibiting effect on the growth of the experimental micro-organisms, no damaging effect on the cultivating components, and no influence on the physical behavior. The polysorbate-80, the lecithin and the agar have practical and reliable neutralizing effect on the corresponding marine organism disinfecting solution. The invention is suitable for evaluating the disinfecting effect of the marine organism disinfecting solution and has the advantages of practical and reliable neutralizing effect on the corresponding marine organism disinfecting solution, no damaging effect on the cultivating components and no influence on the physical behavior, and also has the advantage that the neutralizer or the product of reaction thereof with the marine organism disinfecting solution has no killing effect on the experimental micro-organisms or no inhibiting effect on the growth of the experimental micro-organisms.
Description
Technical field the present invention relates to a kind of in and the sample solution of marine biological disinfectant, its main component is that polysorbate-80, Yelkin TTS, agar are mixed in proportion into mixture.The key problem of disinfection experiment is to estimate the sterilization and the bacteriostatic action of disinfection factor.The effect of evaluating chemical thimerosal must strictly be grasped action time.Thimerosal and microorganism touch after the preset time, must remove its effect immediately, so just can accurately obtain the data of thimerosal action effect; Simultaneously must not contain remaining thimerosal in the sample after taking to sterilize.To prevent that in recover cultivating microorganism from continuing to be subjected to the effect of thimerosal.Therefore, the experiment of any one evaluating chemical thimerosal all must have the reliable method of removing remaining thimerosal.Do not adopt the experiment of removing remaining thimerosal measure, should think serious mistake in design, the result who draws according to such experiment is insecure.The chemistry neutralisation is the thimerosal method of at present the most frequently used removal remnants, and its advantage is that method is simple, and is easy to use, reliable for effect.In and the sample solution of marine biological disinfectant can be used for estimating the disinfection of marine biological disinfectant.Existing thimerosal neutralizing agent kind is a lot, and common have acid, alkali, absorption, dilution, centrifugal a, filtration etc.The above-mentioned screening of all passing through strictness also can't not determined its working concentration; Corresponding thimerosal do not had solid neutralizing effect; Neutralizing agent itself or with the product of thimerosal reaction the experiment microorganism is had killing or suppress the effect of its growth; To cultivating composition destruction is arranged, also influence its physical behavior.Along with the sterilization progress of research, both at home and abroad to the research of neutralizing agent also in continuous development.The disinfection person wishes to have always and a kind ofly can overcome the insufficient neutralizing agent of above-mentioned neutralizing agent.
Background technology the object of the present invention is to provide and a kind ofly can overcome the insufficient marine organisms liquid of existing sterilization neutralizing agent neutralizing agent, compares with other class neutralizing agent to possess following characteristics: corresponding marine biological disinfectant is had solid neutralizing effect; Neutralizing agent itself or with the product of marine biological disinfectant reaction the experiment microorganism is not had killing or suppress the effect of its growth; Do not have destruction to cultivating composition, also do not influence its physical behavior.
Summary of the invention main points of the present invention are to select suitable component, and rationally are mixed, through heat, dissolve, technology such as mixing, cooling, packing, high-temperature sterilization form a kind of in and the sample solution of marine biological disinfectant.
The prescription following (component and weight percent % thereof) that the present invention selects: polysorbate-80 (1.0-3.0); Yelkin TTS (0.1-0.3); Agar (0.5-1.5); Purified water adds to 100.
Polysorbate-80, Yelkin TTS, agar can with the marine biological disinfectant generation chemical reaction of remnants, generate to the experiment microorganism do not kill or suppress its growth effect, do not have destruction to cultivating composition, physical behavior not have the neutralized reaction product that influences.Corresponding marine biological disinfectant had solid neutralizing effect.
The preparation method of product of the present invention is:
1), polysorbate-80, Yelkin TTS are heated in proportion, dissolved.
2), agar is dissolved in the pure water 100 ℃ of heating in proportion, dissolving.Be cooled to 60 ℃.
3), dissolved polysorbate-80, Yelkin TTS are joined in the agar-agar soln of cooling back by proportioning, mix, carry out packing after the cooling, 105 ℃ of sterilizations 15 minutes.Check, warehousing after passing.
The present invention has the following advantages: corresponding marine biological disinfectant is had solid neutralizing effect; Neutralizing agent itself or with the product of marine biological disinfectant reaction the experiment microorganism is not had killing or suppress the effect of its growth; Do not have destruction to cultivating composition, also do not influence its physical behavior.
The present invention is described in further detail below in conjunction with embodiment for embodiment:
Be configured according to following table institute column data (weight percent %) and described step thereof:
Claims (2)
1. the sample solution of the marine biological disinfectant that neutralizes, its main component are that polysorbate-80, Yelkin TTS, agar are mixed in proportion and form; It is characterized in that having following prescription (component and weight percent % thereof): polysorbate-80 (1.0-3.0); Yelkin TTS (0.1-0.3); Agar (0.5-1.5); Purified water adds to 100.
A claim 1 described in and the preparation method of the sample solution of marine biological disinfectant, it is characterized in that having following step:
1), polysorbate-80, Yelkin TTS are heated in proportion, dissolved;
2), agar is dissolved in the pure water 100 ℃ of heating in proportion, dissolving.Be cooled to 60 ℃;
3), dissolved polysorbate-80, Yelkin TTS are joined in the agar-agar soln of cooling back by proportioning, mix, carry out packing after the cooling, 105 ℃ of sterilizations 15 minutes, check, warehousing after passing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710115721A CN101698877A (en) | 2007-12-18 | 2007-12-18 | Sampling solution for neutralizing marine organism disinfecting solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710115721A CN101698877A (en) | 2007-12-18 | 2007-12-18 | Sampling solution for neutralizing marine organism disinfecting solution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101698877A true CN101698877A (en) | 2010-04-28 |
Family
ID=42147342
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200710115721A Pending CN101698877A (en) | 2007-12-18 | 2007-12-18 | Sampling solution for neutralizing marine organism disinfecting solution |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101698877A (en) |
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2007
- 2007-12-18 CN CN200710115721A patent/CN101698877A/en active Pending
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20100428 |