CN101698878A - Neutralization sampling liquid for phenolic disinfectant - Google Patents
Neutralization sampling liquid for phenolic disinfectant Download PDFInfo
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- CN101698878A CN101698878A CN200710115732A CN200710115732A CN101698878A CN 101698878 A CN101698878 A CN 101698878A CN 200710115732 A CN200710115732 A CN 200710115732A CN 200710115732 A CN200710115732 A CN 200710115732A CN 101698878 A CN101698878 A CN 101698878A
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- phenolic disinfectant
- neutralization
- disinfectant
- phenolic
- polysorbate
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Abstract
The invention relates to neutralization sampling liquid for a phenolic disinfectant, which is prepared by mixing the main components of polysorbate-80, lecithin and histidine into a mixture in proportion. The polysorbate-80, the lecithin and the histidine can react with a residual phenolic disinfectant to generate a product of neutralization which has no action of killing an experimental microorganism or inhibiting the growth of the experimental microorganism, has no destructive effect on cultured components and has no influence on physical behaviour. The neutralization sampling liquid has a practical and reliable neutralization effect on the corresponding phenolic disinfectant. The neutralization sampling liquid for the phenolic disinfectant is suitable for evaluating the disinfection of the phenolic disinfectant, and has the following advantages that: the neutralization sampling liquid has the practical and reliable neutralization effect on the corresponding phenolic disinfectant; a neutralizing agent or a product generated by the reaction of the neutralizing agent and the phenolic disinfectant has no action of killing the experimental microorganism or inhibiting the growth of the experimental microorganism, has no destructive effect on the cultured components and has no influence on the physical behavior thereof.
Description
Technical field the present invention relates in a kind of phenolic disinfectant and sample solution, and its main component is that polysorbate-80, Yelkin TTS, Histidine are mixed in proportion into mixture.The key problem of disinfection experiment is to estimate the sterilization and the bacteriostatic action of disinfection factor.The effect of evaluating chemical phenolic disinfectant must strictly be grasped action time.Phenolic disinfectant and microorganism touch after the preset time, must remove its effect immediately, so just can accurately obtain the data of phenolic disinfectant action effect; Simultaneously must not contain remaining phenolic disinfectant in the sample after taking to sterilize.To prevent that in recover cultivating microorganism from continuing to be subjected to the effect of phenolic disinfectant.Therefore, the experiment of any one evaluating chemical phenolic disinfectant all must have the reliable method of removing remaining phenolic disinfectant.Do not adopt the experiment of removing remaining phenolic disinfectant measure, should think serious mistake in design, the result who draws according to such experiment is insecure.The chemistry neutralisation is the phenolic disinfectant method of at present the most frequently used removal remnants, and its advantage is that method is simple, and is easy to use, reliable for effect.Can be used for estimating the disinfection of phenolic disinfectant in the phenolic disinfectant with sample solution.Existing thimerosal neutralizing agent kind is a lot, and common have acid, alkali, absorption, dilution, centrifugal a, filtration etc.The above-mentioned screening of all passing through strictness also can't not determined its working concentration; The corresponding phenolic thimerosal do not had solid neutralizing effect; Neutralizing agent itself or with the product of phenolic disinfectant reaction the experiment microorganism is had killing or suppress the effect of its growth; To cultivating composition destruction is arranged, also influence its physical behavior.Along with the sterilization progress of research, both at home and abroad to the research of neutralizing agent also in continuous development.The disinfection person wishes to have always and a kind ofly can overcome the insufficient neutralizing agent sample solution of above-mentioned neutralizing agent.
Background technology the object of the present invention is to provide and a kind ofly can overcome in the insufficient phenolic disinfectant of existing sterilization neutralizing agent and sample solution, compares with other class neutralizing agent to possess following characteristics: the corresponding phenolic thimerosal is had solid neutralizing effect; Neutralizing agent itself or with the product of phenolic disinfectant reaction the experiment microorganism is not had killing or suppress the effect of its growth; Do not have destruction to cultivating composition, also do not influence its physical behavior.
Summary of the invention main points of the present invention are to select suitable component, and rationally are mixed, through heat, dissolve, technology such as mixing, cooling, packing, high-temperature sterilization forms in a kind of phenolic disinfectant and sample solution.
The prescription following (component and weight percent % thereof) that the present invention selects: polysorbate-80 (1.0-10.0); Yelkin TTS (0.1-0.3); Histidine (0.1-0.3); Purified water adds to 100.
Polysorbate-80, Yelkin TTS, Histidine can with the phenolic disinfectant generation chemical reaction of remnants, generate to the experiment microorganism do not kill or suppress its growth effect, do not have destruction to cultivating composition, physical behavior not have the neutralized reaction product that influences.The corresponding phenolic thimerosal had solid neutralizing effect.
The preparation method of product of the present invention is:
1), polysorbate-80, Yelkin TTS are heated in proportion, dissolved.
2), Histidine is dissolved in the pure water 54 ℃ of heating in proportion, cooling.
3), dissolved polysorbate-80, Yelkin TTS are joined in the Histidine solution of cooling back by proportioning, mix, carry out packing after the cooling, 105 ℃ of sterilizations 15 minutes.Check, warehousing after passing.
The present invention has the following advantages: the corresponding phenolic thimerosal is had solid neutralizing effect; Neutralizing agent itself or with the product of phenolic disinfectant reaction the experiment microorganism is not had killing or suppress the effect of its growth; Do not have destruction to cultivating composition, also do not influence its physical behavior.
The present invention is described in further detail below in conjunction with embodiment for embodiment:
Be configured according to following table institute column data (weight percent %) and described step thereof:
Claims (2)
1. the present invention relates in a kind of phenolic disinfectant and sample solution, its main component is that polysorbate-80, Yelkin TTS, Histidine are mixed in proportion into mixture; It is characterized in that having following prescription (component and weight percent % thereof): polysorbate-80 (1.0-10.0); Yelkin TTS (0.1-0.3); Histidine (0.1-0.3); Purified water adds to 100.
2. in the described phenolic disinfectant of claim 1 and the preparation method of sample solution, it is characterized in that having following step:
1), polysorbate-80, Yelkin TTS are heated in proportion, dissolved;
2), Histidine is dissolved in the pure water 54 ℃ of heating in proportion, cooling;
3), dissolved polysorbate-80, Yelkin TTS are joined in the Histidine solution of cooling back by proportioning, mix, carry out packing after the cooling, 105 ℃ of sterilizations 15 minutes.Check, warehousing after passing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710115732A CN101698878A (en) | 2007-12-18 | 2007-12-18 | Neutralization sampling liquid for phenolic disinfectant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710115732A CN101698878A (en) | 2007-12-18 | 2007-12-18 | Neutralization sampling liquid for phenolic disinfectant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101698878A true CN101698878A (en) | 2010-04-28 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200710115732A Pending CN101698878A (en) | 2007-12-18 | 2007-12-18 | Neutralization sampling liquid for phenolic disinfectant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101698878A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107151688A (en) * | 2017-05-23 | 2017-09-12 | 新乡医学院 | In a kind of public sanitary place air sampling and culture medium |
-
2007
- 2007-12-18 CN CN200710115732A patent/CN101698878A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107151688A (en) * | 2017-05-23 | 2017-09-12 | 新乡医学院 | In a kind of public sanitary place air sampling and culture medium |
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| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20100428 |