CN101380281A - Paeonol bletilla striata polysaccharide clathrate and preparation technique and use thereof - Google Patents
Paeonol bletilla striata polysaccharide clathrate and preparation technique and use thereof Download PDFInfo
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- CN101380281A CN101380281A CNA2008101571993A CN200810157199A CN101380281A CN 101380281 A CN101380281 A CN 101380281A CN A2008101571993 A CNA2008101571993 A CN A2008101571993A CN 200810157199 A CN200810157199 A CN 200810157199A CN 101380281 A CN101380281 A CN 101380281A
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Abstract
The invention pertains to the field of cosmetics, and discloses a paeonol-bletilla striata polysaccharide inclusion compound, a preparation process and the application thereof. Two components of the inclusion compound have the weight percentage as follows: 70%-95% of the bletilla striata polysaccharide and 5%-30% of the paeonol. The inclusion compound can be taken as a cosmetic additive, and has obvious effects of removing spot and whitening skin, delaying skin senility, moistening skin, resisting bacteria and relieving inflammation, and the like, after being added to relevant cosmetic products.
Description
Technical field
The invention belongs to cosmetic field, relate to paeonol bletilla striata polysaccharide clathrate and preparation technology thereof and application.
Background technology
The applicating history of natural plants aspect beauty and skin care is long, promptly occur period as far back as Qin Han, to the Tang Dynasty very in vogue.Material such as contained organic acid, oils and fats, alkaloid, quintessence oil, phlegmatic temperament, protein, aminoacid, vitamins, mineral, trace element, phytohormone all can be to the generation different effect and the influence of human body skin in the plant.Effect, saponin tool decontamination contaminant releasing effect, acid as tannin Bearberry Extract skin have anti-inflammation and sterilization effect, mucopolysaccharide that moisture-keeping function, aminoacid energy skin nutrition are arranged, and reduce wrinkle, and protein are also helpful to strengthening skin elasticity.Along with progress of science and technology, numerous active component also can be extracted and separate, and this has created condition for such plant in the application of natural household chemicals field.
At present, cosmetics industry in people life in occupation of considerable status, wherein functional component come from that the cosmetics of plant are safe in utilization because of it, action temperature and, significantly extremely consumer's favor of effect.A lot of medicinal plants are applied to functional cosmetics production successively, have produced good social and economic benefit.Therefore, using the compound cosmetics additive of various plants active component that the Chinese medicine scientific principle is discussed and modern science and technology prepare more and more comes into one's own in the day chemical industry.
Cortex Moutan is the root bark of Ranunculaceae Paeonia plant Paeonia suffruticosa (Paenoina S uf f ruticosaA ndr).Cold nature, bitter in the mouth, suffering.GUIXIN, liver, kidney channel.Be stated from Shennong's Herbal and classify middle product as, have clearing away heat and cooling blood, the effect of blood circulation promoting and blood stasis dispelling.The Heze, Shandong is the main producing region of Paeonia suffruticosa, and medicinal Paeonia suffruticosa is to introduce a fine variety " Paeonia ostii " from Tongling, Anhui the sixties in 20th century.Cortex Moutan is concocted and to be seen China's beam Dai Taohong scape the earliest and write in " variorum ".Think: " all mallet is broken, removes the heart." record " the Cortex Moutan core is not used as medicine, and need go it " in " Thunder God big gun moxibustion opinion ", the Compendium of Material Medica etc.Radix Cynanchi Paniculati derives from the dry root and rhizome of Mo Luoke plant Radix Cynanchi Paniculati (Cynanchumpaniculatum (Bge.) Kitag), is herbaceos perennial, have wild, the cultivation two kinds.By China's pharmacy monograph Shennong's Herbal is the earliest recorded.Radix Cynanchi Paniculati is warm in nature, and acrid in the mouth has effects such as the removing dampness of dispeling the wind, antalgesic-antipruritic.
Paeonol (paeonol) is one of main active of Chinese medicine Cortex Moutan and Chinese medicine Radix Cynanchi Paniculati.Chemistry 2-hydroxyl by name-4-methoxyacetophenone, pure product are colourless acicular crystal, slightly soluble water can volatilize with steam, is dissolved in ethanol, ether, acetone and other organic solvent.Paeonol has multiple pharmacological effect such as anti-inflammation, antioxidation, antiallergic.Owing to paeonol tryrosinase is had obvious inhibitory action simultaneously, and then can suppress the synthetic of dermal melanin, therefore, have purposes widely at cosmetic field.
The Chinese medicine Pseudobulbus Bletillae (Rhizoma Bletillae) is the dry tuber of the orchid Pseudobulbus Bletillae (Rhizoma Bletillae) (Blettila striata (Thunb.) Reichb.f.), the Pseudobulbus Bletillae (Rhizoma Bletillae) has astringing to arrest bleeding, the effect of detumescence and promoting granulation is widely used in diseases such as treatment hemoptysis, haematemesis, traumatic hemorrhage, sore swollen toxin, pulmonary tuberculosis hemoptysis, ulcer haemorrhage clinically.Major part is a Pseudobulbus Bletillae (Rhizoma Bletillae) glue in the Pseudobulbus Bletillae (Rhizoma Bletillae) tuber, and the main component of Pseudobulbus Bletillae (Rhizoma Bletillae) glue is neutral Pseudobulbus Bletillae polysaccharose.Pseudobulbus Bletillae polysaccharose mean molecule quantity 2800000, form by mannose and glucose polymerisation, the ratio of two kinds of glycosyls is approximately 4:1, three kinds of glycosidic bond: β-1 are arranged, 4-glucose residue (Glc), β-1,4-mannose residue (Man), α-1,6-glucose residue (Glc) are generally based on β type glycosidic bond.Wherein, a plurality of β-1,4-Man can form helical structure, has the potential effect that forms enclose content medicine.The filming performance of Pseudobulbus Bletillae polysaccharose is better than sodium carboxymethyl cellulose and polyvinyl alcohol, is a kind of good natural thickener, is applied to various cosmetics, can substitute various chemical thickening agents, reduces the zest harm to skin.Pseudobulbus Bletillae (Rhizoma Bletillae) rubber powder and Pseudobulbus Bletillae (Rhizoma Bletillae) glucomannoglycan glue are the pharmaceutic adjuvant and the quite promising biomedical materials of medical material, performance brilliance safely and efficiently.
Because paeonol dissolubility in water is very low, the character instability is volatile, sees the easy oxidation Decomposition of light.For water solublity and the stability of improving paeonol, many researcheres have carried out physics, chemical modification to it.Physical modification is meant the chemical constitution that does not change paeonol, with special material it is sealed, and the chemical constitution with respect to the transformation material of chemical modification adds other functional group, and physical modification is more safe and simple, is difficult for producing toxic side effect.
The research report that at present relevant Pseudobulbus Bletillae polysaccharose is a main body enclose paeonol is not seen research as yet.
Summary of the invention
The purpose of this invention is to provide a kind of bioactive substance and stablized, can be used for developing the paeonol bletilla striata polysaccharide clathrate that has bactericidal antiphlogistic, freckle removing and whitening, crease-resistant cosmetics of preserving moisture concurrently.
Another object of the present invention provides the preparation technology of above-mentioned paeonol bletilla striata polysaccharide clathrate.
A further object of the present invention provides the application of above-mentioned paeonol bletilla striata polysaccharide clathrate in cosmetics.
Purpose of the present invention can realize by following measures:
Paeonol bletilla striata polysaccharide clathrate, the weight percent of two kinds of compositions is in this clathrate:
Pseudobulbus Bletillae polysaccharose 70%～95%, paeonol 5%～30%.
Described paeonol bletilla striata polysaccharide clathrate, this clathrate prepares by following method:
A, dissolving: paeonol volume ratio (g/ml, down together) by weight adds 1～3 (w/v) dissolution with solvents doubly, is made into paeonol solution;
B, enclose: it is that 20 ℃～60 ℃ weight percent concentration is 0.5%～20% Pseudobulbus Bletillae polysaccharose aqueous solution that paeonol solution is dropwise added homothermic temperature, stir 1～8h, mixing speed 50rpm～1000rpm, the weight ratio of paeonol and Pseudobulbus Bletillae polysaccharose is 1:2～1:10;
C, drying: be cooled to room temperature, drying gets solid matter;
D, washing, drying: with solid matter by weight volume ratio (g/ml) adopt 1～5 (w/v) solvent wash doubly, centrifugal, precipitation is dry.
Described paeonol bletilla striata polysaccharide clathrate, wherein the dissolution process solvent load be paeonol 0.5～3 (w/v) doubly, solvent is methanol, ethanol, propylene glycol or glycerol.
Described paeonol bletilla striata polysaccharide clathrate, wherein the clathrate process mixing time is 2～6h, mixing speed 100rpm～800rpm, the weight ratio of paeonol and Pseudobulbus Bletillae polysaccharose is 1:2～1:8;
Described paeonol bletilla striata polysaccharide clathrate, wherein drying process adopts lyophilization or spray drying; Adopt spray drying air outlet temperature to be controlled at 65 ℃～85 ℃.
Described paeonol bletilla striata polysaccharide clathrate wherein washs drying process, adopts solid matter 1～5 (w/v) solvent wash doubly, and solvent is petroleum ether, acetone or ether, and is centrifugal, the precipitation lyophilization.
Described paeonol bletilla striata polysaccharide clathrate, wherein Pseudobulbus Bletillae polysaccharose obtains by following preparation technology: Pseudobulbus Bletillae (Rhizoma Bletillae) powder is broken to 20 orders～40 orders and adds water 1:8～1:20 (w/w) in 40 ℃～70 ℃ extraction 2～8h, filter, concentrate, the Pseudobulbus Bletillae (Rhizoma Bletillae) crude polysaccharides that obtains with 75%～95% ethanol precipitation, again through organic solvent washing, the refining Pseudobulbus Bletillae polysaccharose that Protease Treatment obtains; Organic solvent is selected from one or more in ethanol, ethyl acetate, acetone, the ether, and protease is one or more in neutral protease, alkaline protease, papain, the compound protease.
The preparation method of described paeonol bletilla striata polysaccharide clathrate comprises the following steps:
A, dissolving: paeonol adds 1～3 (w/v) dissolution with solvents doubly, is made into paeonol solution; (unit: g/ml, down together)
B, enclose: it is that 20 ℃～60 ℃ weight percent concentration is 0.5%～20% Pseudobulbus Bletillae polysaccharose aqueous solution that paeonol solution is dropwise added homothermic temperature, stir 1～8h, mixing speed 50rpm～1000rpm, the weight ratio of paeonol and Pseudobulbus Bletillae polysaccharose is 1:2～1:10;
C, drying: be cooled to room temperature, drying gets solid matter;
D, washing, drying: solid matter is adopted 1～5 (w/v) solvent wash doubly, and centrifugal, precipitation is dry.
The application of described paeonol bletilla striata polysaccharide clathrate in cosmetics.Wherein, described cosmetics are skin type cream cream cosmetic or washing class cosmetics.
Described application, wherein the percentage by weight of paeonol bletilla striata polysaccharide clathrate in skin type cream cream cosmetic is 0.1%～10%.
Described application, wherein paeonol bletilla striata polysaccharide clathrate shared percentage by weight in washing class cosmetics is 0.5%～15%.
Advantage of the present invention:
The present invention carries out enclose with Pseudobulbus Bletillae polysaccharose to paeonol, utilizes Pseudobulbus Bletillae polysaccharose as main body enclose paeonol, not only can improve the stability and the bioavailability of paeonol, and the biological effect of the two is superposeed to some extent, makes the with better function of clathrate.
1, clathrate process is a physical process, does not change the chemical constitution of paeonol, improves the stability and the water solublity of paeonol.
2, Pseudobulbus Bletillae polysaccharose paeonol clathrate has the removing the necrotic tissue and promoting granulation of anti-inflammation, inhibition melanin and the Pseudobulbus Bletillae (Rhizoma Bletillae) of paeonol simultaneously, and the skin-moisturizing, skin biological activity produces synergistic function.After adding relevant cosmetic product spot dispelling whitening, delaying decrepitude of skin, skin moistening are preserved moisture, anti-inflammation etc. all have remarkable effect.
Bacteriostatic activity is measured
(1) inhibitory action of plant pathogenetic bacteria: adopt inhibition zone method, make plating medium, the bacterium liquid of quantitative debita spissitudo is coated with in culture medium, the filter paper of taking-up behind dry heat sterilization, place the sample of variable concentrations respectively, after soaking 2min, put on the flat board that contains bacterium according to the requirement of sterile working.Put into 37 ℃ of cultivations of the biochemical incubator of electronics.With vernier caliper measurement antibacterial circle diameter D (mm).Repeat for three times.
(2) inhibitory action of plant pathogenic fungi: adopt inhibition zone method, phytopathogen mixes respectively with PDA culture medium and medicine and is prepared into the flat board that pastille is respectively 6,25,100,400 μ g/mL, is contrast with the pure PDA flat board that does not connect bacterium.After the inoculation, put 28 ℃ of cultivations in the biochemical incubator of electronics, survey colony diameter with slide gauge, continuous measurement 6d, each concentration repeats 3 times, gets the mean value calculation suppression ratio of the numerical value of last observation.
Experimental studies results shows (table 1,2): paeonol and clathrate thereof have inhibitory action to plant pathogenic fungi, and the interspecific difference opposite sex is arranged.The inhibitory effect of paeonol and clathrate thereof all presents certain dosage effect, and promptly along with the increase of concentration, inhibitory action is obvious more.We also can see the ratio of enclose experimental group, and enclose experimental group fungistatic effect is not higher.At 400mgL
-1The D-B processed group will exceed 5.1% for the effect of Fructus Solani melongenae sclerotium than the DA processed group with concentration.Equally, at 400mgL
-1The D-B processed group will exceed 10.1% for the effect of N8 than the DA processed group with concentration.
Table 1 paeonol and clathrate thereof to the inhibitory action of Fructus Solani melongenae sclerotium (X ± S, n=9)
Description of drawings
Fig. 1 is the differential thermogram of paeonol, Pseudobulbus Bletillae polysaccharose, paeonol and Pseudobulbus Bletillae polysaccharose physical mixture, paeonol and bletilla striata polysaccharide clathrate.
The specific embodiment
The invention will be further elaborated by the following examples.
Pseudobulbus Bletillae polysaccharose among the embodiment prepares by following method:
Pseudobulbus Bletillae (Rhizoma Bletillae) crude polysaccharides: Pseudobulbus Bletillae (Rhizoma Bletillae) powder is broken to 20 orders～40 orders, adds 1:8～1:20 (w/w) water doubly and extracts 2～8h in 40 ℃～70 ℃, filters, concentrates, the Pseudobulbus Bletillae (Rhizoma Bletillae) crude polysaccharides that obtains with 75%～95% ethanol precipitation.
Refining Pseudobulbus Bletillae polysaccharose: take by weighing Pseudobulbus Bletillae (Rhizoma Bletillae) crude polysaccharides, adding concentration by weight of material volume ratio 1:4 is 70% alcoholic solution (being Pseudobulbus Bletillae (Rhizoma Bletillae) crude polysaccharides 100g, 70% ethanol 400ml), sets bath temperature to 70 ℃, heating 1h, cooling then.Vacuum filtration is 95% washing with alcohol three times with concentration.Accurately take by weighing weighing apparatus dry weight Pseudobulbus Bletillae (Rhizoma Bletillae) crude polysaccharides sample after the washing with alcohol, ratio in 30ml water/g washing back weighing apparatus dry weight Pseudobulbus Bletillae (Rhizoma Bletillae) crude polysaccharides adds entry, place 50 ℃ of water-baths, constantly stir, make it abundant dissolving, add neutral protease by 0.5% of washing back weighing apparatus dry weight Pseudobulbus Bletillae (Rhizoma Bletillae) crude polysaccharides weight, regulate pH6.5-7.0,50 ℃ of complete hydrolysis 20h.70 ℃ of enzyme denaturing of enzymolysis solution are handled 10min, use High speed refrigerated centrifuge 6000r/min, centrifugal 15min.Inclining supernatant, adds 95% ethanol of 4 times of supernatant volumes, precipitate with ethanol 4h final vacuum sucking filtration, and the Pseudobulbus Bletillae (Rhizoma Bletillae) glue purity behind the vacuum filtration is 95% washing with alcohol 2 times, washing with acetone 3 times, ether washing 3 times, filter is done, and stores after drying 2h for 60 ℃.
Protease also can adopt following protease to handle:
Enzyme class optimum temperature (℃) pH scope consumption (%)
Papain 55 5.5 ± 0.5 0.2
Alkaline protease 65 7.0-8.0 0.6
Compound protease 48 8.0 ± 0.5 0.15
The organic solvent of washing usefulness can adopt one or more in ethanol, ethyl acetate, acetone, the ether.
Embodiment 1:
Get paeonol 50g,, dropwise add the 4L of 40 ℃ of constant temperature with the anhydrous alcohol solution of 100mL, in the 5wt% Pseudobulbus Bletillae polysaccharose aqueous solution, 300rpm stirs 5h, is cooled to room temperature, lyophilization, get the white powder thing, with the petroleum ether of 2 times of volumes of white powder thing, the centrifugal supernatant of abandoning, precipitation lyophilization, get Powdered thing 181.8g, be clathrate.Recovery rate is 72.7%, and envelop rate is 30.2%.
Embodiment 2:
Get paeonol 50g,, dropwise add the 3L of 55 ℃ of constant temperature with the absolute methanol dissolving of 80mL, in the 10wt% splendid achnatherum polysaccharide solution, 600rpm stirs 3h, is cooled to room temperature, spray drying, the air outlet temperature is controlled at 65 ℃～70 ℃, get the white powder thing, with the washing with acetone of 3 times of volumes of white powder thing, the centrifugal supernatant of abandoning, the precipitation lyophilization gets Powdered thing 267.1g and is clathrate.Recovery rate is 76.3%, and envelop rate is 32.4%.
The evaluation of paeonol and clathrate differential analysis instrument thereof:
Get paeonol, Pseudobulbus Bletillae polysaccharose, physical mixture, clathrate totally 4 kinds of samples carry out differential scanning calorimeter at differential thermal analyzer (CDR-4P type): take by weighing 5.0mg left and right sides sample, range is ± 50 μ V, the intensification scope is 40 ℃-400 ℃, and heating rate is 10 ℃/min, with Al
2O
3Be reference, make the DTA collection of illustrative plates.
As can be seen from Figure 1: the mixture collection of illustrative plates of Pseudobulbus Bletillae polysaccharose paeonol also is the stack of unification compound collection of illustrative plates basically, variation has all taken place on the clathrate collection of illustrative plates, different with the collection of illustrative plates of Pseudobulbus Bletillae polysaccharose, there are not tangible paeonol endothermic peak and fusion-decomposition peak, so formed clathrate yet.
Embodiment 3: contain using method and the ratio of paeonol bletilla striata polysaccharide clathrate cosmetics additive in skin type cream frost
Skin cream prescription: (ratio is a percentage by weight)
Material title ratio composition classification and function
A phase: octadecanol 2%～5% high fatty alcohol
Single sweet vinegar 1%～2% high fatty alcohol
Angle huge legendary turtle alkane 2%～4% hydrocarbons
White oil 2%～4% hydrocarbons
Palm fibre is put isopropyl propionate 0.5%～1.5% Ester
The stearic alcohol ether of polyoxyethylene (2) (Brij72) 0.15%～0.3% surfactant
The stearic alcohol ether of polyoxyethylene (21) (Brij721) 0.1%～0.2% surfactant
2,6-di-tert-butyl-4-methy phenol (BHT) 0.01%～0.02% antioxidant
Nipalgin third 0.003%～0.005% antiseptic
Dimethicone 0.5%～1.5%
Isopropyl stearate 0.5%～1.5% Ester
B phase: propylene glycol 2%～4% wetting agent
Glycerol 4%～6% wetting agent
Paeonol bletilla striata polysaccharide clathrate 3%～6% active component
Metagin 0.01%～0.02% antiseptic
C phase: essence 0.01%～0.02%
Triethanolamine (TEA) 0.01%～0.02% pH regulator agent
The deionized water surplus
Production technology:
A is heated with stirring to 90 ℃ mutually, is incubated 10 minutes.B is heated with stirring to 50 ℃ mutually, and A, B merge mutually, and emulsifying is 30 minutes in emulsifying device, is cooled to 60 ℃, adds C phase material, after stirring, and is chilled to 40 ℃, discharging.Chemically examine after testing qualified after, pack.
Embodiment 4: contain using method and the ratio of paeonol bletilla striata polysaccharide clathrate cosmetics additive in washing class cosmetics
The prescription of child's cleaning product (ratio is a percentage by weight):
Paeonol bletilla striata polysaccharide clathrate 5%～10%
Natural surfactant (tea saponin) 5%～10%
Ester (palm fibre is put isopropyl propionate) 2%～4%
Wetting agent (glycerol) 2%～4%
Antiseptic (nipalgin third) 0.003%～0.005%
Essence 0.005%～0.01%
The deionized water surplus
Production technology:
Formula components 50 ℃ of dissolvings evenly outgases, and slowly cools to 30 ℃, discharging.Chemically examine after testing qualified after, pack.
(product that paeonol bletilla striata polysaccharide clathrate among the embodiment 3 and 4 adopts embodiment 1 or 2 all can)
Claims (10)
1, paeonol bletilla striata polysaccharide clathrate is characterized in that the weight percent of two kinds of compositions in this clathrate is:
Pseudobulbus Bletillae polysaccharose 70%～95%, paeonol 5%～30%.
2, paeonol bletilla striata polysaccharide clathrate according to claim 1 is characterized in that this clathrate prepares by following method:
A, dissolving: paeonol volume ratio (g/ml) by weight adds 1～3 (w/v) dissolution with solvents doubly, is made into paeonol solution;
B, enclose: it is that 20 ℃～60 ℃ weight percent concentration is 0.5%～20% Pseudobulbus Bletillae polysaccharose aqueous solution that paeonol solution is dropwise added homothermic temperature, stir 1～8h, mixing speed 50rpm～1000rpm, the weight ratio of paeonol and Pseudobulbus Bletillae polysaccharose is 1:2～1:10;
C, drying: be cooled to room temperature, drying gets solid matter;
D, washing, drying: with solid matter by weight volume ratio (g/ml) adopt 1～5 (w/v) solvent wash doubly, centrifugal, precipitation is dry.
3, paeonol bletilla striata polysaccharide clathrate according to claim 2, it is characterized in that the dissolution process solvent load be paeonol 0.5～3 (w/v) doubly, solvent is methanol, ethanol, propylene glycol or glycerol; The clathrate process mixing time is 2～6h, mixing speed 100rpm～800rpm, and the weight ratio of paeonol and Pseudobulbus Bletillae polysaccharose is 1:2～1:8;
4, paeonol bletilla striata polysaccharide clathrate according to claim 2 is characterized in that drying process adopts lyophilization or spray drying; Adopt spray drying air outlet temperature to be controlled at 65 ℃～85 ℃.
5, paeonol bletilla striata polysaccharide clathrate according to claim 2 is characterized in that washing drying process, adopts solid matter 1～5 (w/v) solvent wash doubly, and solvent is petroleum ether, acetone or ether, and is centrifugal, the precipitation lyophilization.
6, paeonol bletilla striata polysaccharide clathrate according to claim 1, it is characterized in that Pseudobulbus Bletillae polysaccharose obtains by following preparation technology: Pseudobulbus Bletillae (Rhizoma Bletillae) powder is broken to 20 orders～40 orders and adds water 1:8～1:20 (w/w) in 40 ℃～70 ℃ extraction 2～8h, filter, concentrate, the Pseudobulbus Bletillae (Rhizoma Bletillae) crude polysaccharides that obtains with 75%～95% ethanol precipitation, again through organic solvent washing, the refining Pseudobulbus Bletillae polysaccharose that Protease Treatment obtains; Wherein organic solvent is selected from one or more in ethanol, ethyl acetate, acetone, the ether, and protease is one or more in neutral protease, alkaline protease, papain, the compound protease.
7, the preparation method of paeonol bletilla striata polysaccharide clathrate as claimed in claim 1 is characterized in that comprising the following steps:
A, dissolving: paeonol volume ratio (g/ml) by weight adds 1～3 (w/v) dissolution with solvents doubly, is made into paeonol solution;
B, enclose: it is that 20 ℃～60 ℃ weight percent concentration is 0.5%～20% Pseudobulbus Bletillae polysaccharose aqueous solution that paeonol solution is dropwise added homothermic temperature, stir 1～8h, mixing speed 50rpm～1000rpm, the weight ratio of paeonol and Pseudobulbus Bletillae polysaccharose is 1:2～1:10;
C, drying: be cooled to room temperature, drying gets solid matter;
D, washing, drying: with solid matter by weight volume ratio (g/ml) adopt 1～5 (w/v) solvent wash doubly, centrifugal, precipitation is dry.
8, the application of the described paeonol bletilla striata polysaccharide clathrate of claim 1 in cosmetics.
9, application according to claim 8 is characterized in that cosmetics are skin type cream cream cosmetic or washing class cosmetics.
10, application according to claim 9 is characterized in that the percentage by weight of paeonol bletilla striata polysaccharide clathrate in skin type cream cream cosmetic is 0.1%～10%; Paeonol bletilla striata polysaccharide clathrate shared percentage by weight in washing class cosmetics is 0.5%～15%.
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| CN103156783A (en) * | 2011-12-15 | 2013-06-19 | 中华全国供销合作总社南京野生植物综合利用研究院 | Formula and preparation technology of skin care product containing plant silk protein |
| CN103203208A (en) * | 2013-04-22 | 2013-07-17 | 中华全国供销合作总社南京野生植物综合利用研究所 | Preparation technology of paeonol and bletilla polysaccharide microcapsules, and application of prepared product thereof |
| CN105581964A (en) * | 2016-02-04 | 2016-05-18 | 姚蓉 | Product for rejuvenating and beautifying and preparation method thereof |
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| CN105640797A (en) * | 2016-02-04 | 2016-06-08 | 向君民 | Application of dihydromyricetin and bletilla striata polysaccharide composition in beautifying and whitening |
| CN105663000A (en) * | 2016-02-04 | 2016-06-15 | 湖南农业大学 | Rhizome bletillae moisturizing gel product and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103156783A (en) * | 2011-12-15 | 2013-06-19 | 中华全国供销合作总社南京野生植物综合利用研究院 | Formula and preparation technology of skin care product containing plant silk protein |
| CN103203208A (en) * | 2013-04-22 | 2013-07-17 | 中华全国供销合作总社南京野生植物综合利用研究所 | Preparation technology of paeonol and bletilla polysaccharide microcapsules, and application of prepared product thereof |
| CN103203208B (en) * | 2013-04-22 | 2014-12-31 | 中华全国供销合作总社南京野生植物综合利用研究所 | Preparation technology of paeonol and bletilla polysaccharide microcapsules, and application of prepared product thereof |
| CN105581964A (en) * | 2016-02-04 | 2016-05-18 | 姚蓉 | Product for rejuvenating and beautifying and preparation method thereof |
| CN105640792A (en) * | 2016-02-04 | 2016-06-08 | 王婧 | Application of dihydromyricetin in beautifying and freckle removing products |
| CN105640797A (en) * | 2016-02-04 | 2016-06-08 | 向君民 | Application of dihydromyricetin and bletilla striata polysaccharide composition in beautifying and whitening |
| CN105663000A (en) * | 2016-02-04 | 2016-06-15 | 湖南农业大学 | Rhizome bletillae moisturizing gel product and preparation method thereof |
| CN105640797B (en) * | 2016-02-04 | 2018-07-17 | 广州金纯化妆品有限公司 | Dihydromyricetin and application of the bletilla polysaccharide composition in beautifying whitening |
| CN105640792B (en) * | 2016-02-04 | 2018-08-10 | 长沙爱扬医药科技有限公司 | Dihydromyricetin is in beauty except the application in spot product |
| CN105997964A (en) * | 2016-04-18 | 2016-10-12 | 中华全国供销合作总社南京野生植物综合利用研究院 | Efficacy type sterilization and disinfection liquid containing natural citral-bletilla striata polysaccharide polymer and preparation method thereof |
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