CN101248750B - Paddy rice dual-purpose genic male sterile line single-season with regeneration cold water string irrigating seed breeding method - Google Patents
Paddy rice dual-purpose genic male sterile line single-season with regeneration cold water string irrigating seed breeding method Download PDFInfo
- Publication number
- CN101248750B CN101248750B CN2007103010039A CN200710301003A CN101248750B CN 101248750 B CN101248750 B CN 101248750B CN 2007103010039 A CN2007103010039 A CN 2007103010039A CN 200710301003 A CN200710301003 A CN 200710301003A CN 101248750 B CN101248750 B CN 101248750B
- Authority
- CN
- China
- Prior art keywords
- water
- string
- season
- temperature
- sterile line
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The invention belongs to the technical field of science and engineering, in particular to a propagation method for rice dual-purpose genic male sterile line seeds with the pattern of high-temperature sterile, low-temperature fertile or long day high-temperature sterile, short day low-temperature fertile. The technique of the method includes: (1) a propagation base is required not only to meet theneed of normal growing development of the conventional rice, but also to have sufficient water source at temperature of 17-19 DEG C and plant no other rice variety in the periphery of 300m; (2) the seeding dates of the first rice crop are arranged on March,15th-20th, (3) the water temperature of the field is maintained between 20-21 DEG C for continuous 15 days from the fourth period of the differentiation of the first young ears, an irrigation depth is based on young ears submergence and the growth process of the young ears to irrigate from shallow to deep until the depth is up to 20m; (4) the stubble height of rice stub after first harvestry is 20-25cm, the water temperature of the field is maintained between 20.5-21.5 DEG C for continuous 16-20 days from the fourth period of the differentiation of the second young ears, the irrigation depth is up to 28cm from shallow to deep; (5) abnormal plants and inferior plants are strictly removed. By specifying the technique, the purpose of producing high-yield, high-quality and high efficiency rice dual-purpose genic male sterile line seeds can be achieved.
Description
Technical field:
The invention belongs to seed scientific and engineering technical field, the seed propagation method that specifically a kind of high temperature is sterile, low temperature can be educated or long day high temperature is sterile, short day low temperature can be educated the paddy rice dual-purpose caryon sterile line of type.
Background technology:
The key problem in technology of double-hybrid rice strains be to cultivate a kind of can the safe production of hybrid seeds, again can numerous kind of successful dual purpose genic male sterile line.Current, the sterile conversion origin temp of the temperature sensitive dual purpose genic male sterile line of the light of China's seed selection is generally at 23-24 ℃, such male sterile line, even when carrying out hybrid seeding in full summer, still may be subjected to the short-term low temperature effect, fertility is fluctuateed, cause hybridization seed production purity to descend, even production of hybrid seeds failure.Therefore, the effective ways of raising two line method production of hybrid seeds safety are the dual purpose genic male sterile lines of cultivating critical temperature of sterility low (22-23 ℃).Yet, be subjected to the restriction of the critical low temperature of physiology, the educated temperature range of the dual purpose genic male sterile line fertility-sensitive period of this low critical temperature of sterility very narrow (19-21 ℃), under the natural conditions numerous kind very the difficulty.Usually adopt fertility-sensitive period low-temperature cold water string irrigation technology to carry out numerous kind of male sterile line.But the paddy rice dual-purpose caryon sterile line is very strict to the requirement of cold water string irrigating, careless slightlyly will cause numerous kind of failure.Therefore, the numerous kind of problem that solves this class dual purpose genic male sterile line just seems particularly important.
On the other hand, because ripening rate own is lower in the sterile line propagation process, storing in the rice stem stalk of harvesting back has a large amount of unspent nutriments, so the regeneration capacity of dual purpose genic male sterile line is strong especially.If can make full use of this characteristic, carry out again one season male sterile line regenerate numerous kind, then can improve numerous kind of benefit of dual purpose genic male sterile line greatly.
Summary of the invention:
The objective of the invention is to overcome numerous kind of difficulty of paddy rice dual-purpose caryon sterile line, utilize low temperature can recover male sterile line fertility and the strong characteristic of male sterile line regeneration capacity, propose a kind ofly to be suitable for that high temperature is sterile, low temperature can be educated or sterile, the short day low temperature of long day high temperature can be educated type male sterile line head season and regeneration equal stable and high yields of season, breeding technique efficiently, numerous kind output and benefit can be increased, the purity of breeding seed can be improved again.
Paddy rice dual-purpose caryon sterile line head season provided by the invention adds the regeneration cold water string irrigating breeding technique and comprises: the selection in suitable numerous kind of base, the requirement at cold-penetration water source, cold-penetration breeding irrigation and drainage facility, cold-penetration is arranged season, the cultivation management in paddy rice dual-purpose caryon sterile line head season and cold water string irrigating technology, the cultivation management and the cold water string irrigating technology in regeneration season.
Wherein said breeding base requires its peripheral 300m can not plant other paddy rice with interior, and perhaps fringe phase beginning of fringe phase beginning of other paddy rice and breeding male sterile lines differs more than the 25d in the multiplication plot.The illumination abundance, temperature is suitable, the area that can satisfy conventional rice normal growth, growth.
Wherein said cold-penetration water source requires to possess breeding field water inlet water temperature and reaches 17-19 ℃ sufficient water source.
Wherein said cold-penetration breeding irrigation and drainage facility requires to satisfy 17-19 ℃ of breeding field water inlet water temperature, and the delivery port temperature is controlled at and is lower than about 1 ℃ of breeding male sterile lines critical temperature of sterility.Many mouthfuls of water inlets are taked in breeding field piece list filling, single, the setting of multiple outlets, and the discharge outlet water outlet is introduced drainage ditch by branch canal and is discharged.Breeding field ridge requirement for height is higher than the fringe cusp height of dual purpose genic male sterile line ear differentiation VI phase, generally should be higher than 25cm.
Wherein said cold-penetration is arranged mating season, according to male sterile line breeding time length and the safe full heading time in regeneration season require to arrange sowing time, generally be arranged in seeding head season on March 15 to March 20 in the Hunan.
Wherein said season cultivation management, require male sterile line leaf age 5-6 leaf to transplant, the specification of planting of 16cm * 20cm or 13cm * 20cm is adopted in appropriate dense planting usually, the rice shoot of 1 paddy is inserted in every cave.Plant the row to the water (flow) direction basically identical, be beneficial to improve water velocity; Every about 10 row, stay the aisle, the unimpeded and farming operation of current when being beneficial to cold-penetration.Carry out when handling except that cold-penetration the irrigation of low temperature deep water, all the other cultivation managements carry out in the usual way.Omnidistance attention control damage by disease and insect is particularly strengthened banded sclerotial blight and rice fulgorid control in a later stage in season.Rob fine weather harvesting during grain maturity 85-90%.
The cold water irrigation management of season.Reach the IV phase (female stamen idiophase) from season colony's 5% ear differentiation and carry out low-temperature cold water and irrigate, kept 20～21 ℃ of field water temperature in continuous 15 days.Water irrigating depth is as the criterion to flood young fringe fully, and adds gradually according to young fringe development progress and to be deep to 20cm.Draining dry field immediately after season, cold water string irrigating finished is then in conjunction with irritating the every 667m of shallow water again
2Sealing fertilizer 5kg; Preceding 7-10 days of harvesting, every 667m
2Execute urea 15kg and make short bud fertilizer; Later stage keeps the field moistening.
Wherein said regeneration season cultivation management requires a season harvesting to stay stake height 20-25cm; Rehydration as early as possible after the harvesting, shallow water is diligent irritates; And the 2nd day every 667m after receiving in season
2Impose 20kg urea; Take the circumstances into consideration to prevent and treat field disease, insect pests such as banded sclerotial blight, rice fulgorid according to the field situation.
The management of regeneration season cold water irrigation.Regrowth ear differentiation when 10% is in IV and begins string during the phase and irritate cold water, water irrigating depth with regeneration fringe developmental process from shallow to deep, string is irritated the later stage water irrigating depth and is reached 28cm, string was irritated 16-20 days continuously.When the cold-penetration phase stops string and irritates when a certain period temperature is lower than 20 ℃, and daily mean temperature is lower than 21 ℃ of whole days and stops to irrigate, and the string filling phase requires the field water temperature to remain on 20.5-21.5 ℃.
Embodiment:
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
Embodiment 1: the paddy rice dual-purpose caryon sterile line C815S with applicant's seed selection is an example, and narration adds the embodiment of regeneration cold water string irrigating breeding technique a season.
1. male sterile line characteristic
C815S is the paddy rice dual-purpose caryon sterile line that paddy rice Science Institute of Agricultural University Of Hunan breeds, 22.0 ℃ of its critical temperature of sterility.Handle more than 7 days at 20-21 ℃ of low-temperature cold water of fertility-sensitive period daily mean temperature, can make its fertility recover normal substantially.
2. a season adds the regeneration cold water string irrigating breeding technique
1) a season cultivation: carried out the cold water string irrigating breeding of C815S in 2006 in Chenzhou, Hunan.The cavern water sewage farming piece of selecting 18 ℃ of water temperatures all the year round is as numerous kind of base.Sowing on March 25, male sterile line leaf age 5-6 leaf is transplanted, and adopts the specification of planting of 16cm * 20cm, and the rice shoot of 1 paddy is inserted in every cave.Plant the row to the water (flow) direction basically identical, every 10 the row about stay the aisle.Reach the IV phase (female stamen idiophase) from a season 5% ear differentiation, used cold water string irrigating continuously 15 days, the field water temperature remains on 20～21 ℃.Water irrigating depth is deepened to 20cm gradually according to young fringe development progress.Draining open field immediately after cold-penetration finishes, and in conjunction with irritating the every 667m of shallow water again
2Impose composite fertilizer 5kg; 7-10 days every 667m before the harvesting
2Execute urea 15kg and make short bud fertilizer, alternately to keep the field moistening by doing temperature thereafter; Begin harvesting during the grain maturity 85-90% of colony; Omnidistance prevention and elimination of disease and pests is particularly strengthened banded sclerotial blight and rice fulgorid control in a later stage in season.Respectively at carrying out the field removal of impurities before tillering stage, booting stage, heading stage, stage of yellow ripeness and the harvesting.
2) regeneration season cultivation: stake height 20cm is stayed in a season harvesting; Receive the 2nd day every 667m in back
2Impose 20kg urea; Take the circumstances into consideration to prevent and treat banded sclerotial blight and rice fulgorid according to the field situation.Enter ear differentiation IV at 10% regeneration fringe and begin cold water string irrigating during the phase, water irrigating depth is deepened to 28cm gradually according to young fringe development progress, and string was irritated 18 days continuously.
3. implementation result
General every 667m of season
2Output reaches 250kg, regeneration every 667m of season
2Output reaches 150kg, and two seasons added up to every 667m
2Output can reach more than the 400kg.Compare with current common sterile line propagation, the one, regeneration season less investment has significantly reduced production cost; The 2nd, during regeneration season cold-penetration, avoided the paddy rice water use peak, the cold water resource is fully used; The 3rd, regeneration numerous kind of season can effectively be avoided peripheral external paddy pollen string powder, and through the strict removal of impurities of excessive season, improved seed purity; The 4th, the fertility-sensitive period of dual purpose genic male sterile line in regeneration season with light sensitive characteristic is under the shorter illumination condition, its critical temperature of sterility can raise to some extent, the part dual purpose genic male sterile line has regeneration and educates characteristic again in addition, so the temperature sensitive dual purpose genic male sterile line of light generally can be obtained higher regeneration reproductive output.
Claims (6)
1. a paddy rice dual-purpose caryon sterile line head season adds the regeneration cold water string irrigating breeding method, comprise the selection in suitable numerous kind of base, the requirement at cold-penetration water source, cold-penetration breeding irrigation and drainage facility, cold-penetration is arranged season, the cultivation management in paddy rice dual-purpose caryon sterile line head season and cold water string irrigating technology, the cultivation management and the cold water string irrigating technology in regeneration season is characterized in that:
Described paddy rice dual-purpose caryon sterile line is C815S;
The cultivation management in described paddy rice dual-purpose caryon sterile line head season and the concrete operations of cold water string irrigating technology are: when head season 5% ear differentiation of paddy rice dual-purpose caryon sterile line reaches the IV phase, be that female stamen is during the idiophase, begin string and irritate cold water, water irrigating depth from shallow to deep, be as the criterion to flood young fringe, irritate the later stage to string, water irrigating depth reaches 20cm, and string was irritated 15 days continuously; When string was irritated, many mouthfuls of water inlets will be taken the circumstances into consideration when multiple outlets, temperature of irrigation water are lower than 18 ℃ to stop to irritate, and water temperature is low more, and it is long more to stop the filling time, makes whole water in field temperature remain on 20～21 ℃; Season, cold water string irrigating answered the draining open field, every 667m when irritating shallow water again after finishing
2Sealing fertilizer 5kg; Gather in preceding 7～10 days every 667m
2Impose and make short bud fertilizer about urea 15kg; Season, late growth stage kept the field moistening;
The cultivation management in described regeneration season and the concrete operations of cold water string irrigating technology are: the regrowth ear differentiation when 10% is in the IV phase, be that female stamen is during the idiophase, begin string and irritate cold water, water irrigating depth with regeneration fringe developmental process from shallow to deep, string filling later stage water irrigating depth reaches 28cm, and string was irritated 16～20 days continuously, stops the string filling when a certain period temperature is lower than 20 ℃ when the cold-penetration phase, and daily mean temperature is lower than 21 ℃ of whole days to be stopped to irrigate, and the string filling phase requires the field water temperature to remain on 20.5～21.5 ℃.
2. breeding method according to claim 1 is characterized in that male sterile line is arranged in 3 months sowing time, the last ten-days period, and water is educated or non-irrigated seedling, and film covers, about 30 days length of time rice seedlings grow, and transplanting specification 16cm * 20cm or 13cm * 20cm.
3. breeding method according to claim 1 it is characterized in that breeding spot periphery 300m can not plant other rice varieties with interior place, or other kinds and the male sterile line of plantation staggers more than 25 days flowering stage.
4. 1 described breeding method as requested, the pour water cold water of rice dual purpose genic male sterile line of it is characterized in that being used to go here and there is large reservoir bottom water, cavern water or well water, water temperature requires 17～19 ℃.
5. breeding method according to claim 1 is characterized in that first rice crop grain maturity reaches at 85% o'clock and begins harvesting, stays stake height 20～25cm; Harvested the 2nd day, every 667m
2Execute the urea 20kg bud fertilizer of going bail for.
6. breeding method according to claim 1, before the beginning fringe, according to strain shape, Ye Se and growing way impurity elimination once, fringe phase beginning rogues 3～4 times according to particle shape, bran point look, the continuous removal of impurities of fertility, carries out an impurity elimination before the harvesting again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007103010039A CN101248750B (en) | 2007-12-10 | 2007-12-10 | Paddy rice dual-purpose genic male sterile line single-season with regeneration cold water string irrigating seed breeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007103010039A CN101248750B (en) | 2007-12-10 | 2007-12-10 | Paddy rice dual-purpose genic male sterile line single-season with regeneration cold water string irrigating seed breeding method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101248750A CN101248750A (en) | 2008-08-27 |
| CN101248750B true CN101248750B (en) | 2010-11-03 |
Family
ID=39952496
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007103010039A Expired - Fee Related CN101248750B (en) | 2007-12-10 | 2007-12-10 | Paddy rice dual-purpose genic male sterile line single-season with regeneration cold water string irrigating seed breeding method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101248750B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102067796A (en) * | 2010-10-18 | 2011-05-25 | 曾翔 | New method for regulating and controlling water temperature in breeding process of low temperature sensitive sterile line of paddy |
| CN102119640A (en) * | 2010-11-19 | 2011-07-13 | 湖南农业大学 | Method for producing rice dual-purpose nucleic sterile line original seed |
| CN102498997B (en) * | 2011-11-11 | 2014-02-26 | 湖南师范大学 | Method of screening nucleic sterile plants of photo-thermo-sensitive nucleic sterile rice and method of propagating core seeds |
| CN104186306A (en) * | 2013-12-19 | 2014-12-10 | 国家粳稻工程技术研究中心 | Japonica photoperiod-temperature sensitive genetic male sterile line L62S breeding technology by utilization of string irrigation with cold water and special climate in Hainan |
| CN104170727A (en) * | 2013-12-19 | 2014-12-03 | 国家粳稻工程技术研究中心 | Method for safely breeding light and temperature sensitive genic sterile line by using Hainan climate conditions |
| CN103828647B (en) * | 2014-01-03 | 2016-05-11 | 上海市农业科学院 | The ecological control method of a kind of auxiliary insecticide control white backed planthopper |
| CN105028075A (en) * | 2015-07-27 | 2015-11-11 | 云南省农业科学院粮食作物研究所 | Rapid generation-adding method for rice breeding |
-
2007
- 2007-12-10 CN CN2007103010039A patent/CN101248750B/en not_active Expired - Fee Related
Non-Patent Citations (8)
| Title |
|---|
| 何强等.水稻光温敏核不育系C815S的繁殖特性研究.杂交水稻21 4.2006,21(4),29-30. |
| 何强等.水稻光温敏核不育系C815S的繁殖特性研究.杂交水稻21 4.2006,21(4),29-30. * |
| 向关伦等.水稻两用核不育系再生复育及利用评价.贵州农业科学28 3.2000,28(3),10-11. |
| 向关伦等.水稻两用核不育系再生复育及利用评价.贵州农业科学28 3.2000,28(3),10-11. * |
| 杨文才等.水稻低温敏两用不育系陆18S冷灌繁殖技术.种子23 11.2004,23(11),79-80. |
| 杨文才等.水稻低温敏两用不育系陆18S冷灌繁殖技术.种子23 11.2004,23(11),79-80. * |
| 贺治洲等.水稻籼型两用核不育系种子繁殖研究进展.作物研究 5.2006,(5),383-392. |
| 贺治洲等.水稻籼型两用核不育系种子繁殖研究进展.作物研究 5.2006,(5),383-392. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101248750A (en) | 2008-08-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101248750B (en) | Paddy rice dual-purpose genic male sterile line single-season with regeneration cold water string irrigating seed breeding method | |
| CN104186270B (en) | A kind of facility grape Forcing cultivation method | |
| CN104186149B (en) | Muskmelon lateralbine regeneration continuous fruit setting method | |
| JP2011142903A (en) | Method for raising seedlings of peony | |
| CN101044834B (en) | Method for producing hybrid rice seeds of light-temperature sensitive male sterility line by using low-temperature or short-day low temperature | |
| CN100571503C (en) | A kind of elaeagnus mollis asexual reproduction method | |
| CN105724012A (en) | Rice field-upland field rotation method for achieving no-tillage double cropping of late rice in spring maize field | |
| CN103718783A (en) | Greenhouse watermelon cultivation method | |
| CN101697703B (en) | Method for bickiron inoculation of buds symbiotic to root fungi for culturing seedlings of Chinese chestnut | |
| CN109588239A (en) | A kind of multiple rice regeneration rice breeding method | |
| CN109429853A (en) | A kind of Propagation of Actinidia Arguta By Green Cutting method | |
| CN101044835B (en) | Method for producing hybrid rice seeds of light-temperature sensitive male sterility line under higher temperature or long-day high temperature | |
| CN107006366A (en) | A kind of method that big mass screening cultivates high temperature resistance paddy rice | |
| CN106234126A (en) | The cultural method of booth Fructus Pruni pseudocerasi | |
| CN113348996A (en) | Cultivation method for improving seedling rate and yield of ratoon rice in hilly area | |
| CN101124886B (en) | Hybrid seed production method using cotton sterile male line and recovery line to processing ratoon regeneration | |
| CN101828523B (en) | Seed production method of hybrid rice | |
| CN107155447A (en) | A kind of method of shallot fast breeding | |
| CN104303765B (en) | The high-yield planting method of the stem of noble dendrobium | |
| CN105993509A (en) | Interplanting method for cucumbers and corn | |
| CN113207537B (en) | Grafting breeding method for mountain pepper seedlings Shan Yasui | |
| CN104871922A (en) | Method for cultivating kiwi fruits | |
| CN108496680A (en) | A kind of High-quality Cultivation method of Kiwi berry | |
| CN114568274A (en) | Double-fork seedling growing method for solanaceous fruit crops | |
| CN104303744A (en) | Cultivation and management method for square watermelons |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: HUNAN AGRICULTURAL UNIVERSITY Free format text: FORMER OWNER: CHEN LIYUN Effective date: 20100803 Free format text: FORMER OWNER: XIAO YINGHUI TANG WENBANG |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 410128 RICE RESEARCH INSTITUTE, HUNAN AGRICULTURAL UNIVERSITY, FURONG DISTRICT, CHANGSHA CITY, HUNAN PROVINCE TO: 410128 HUNAN AGRICULTURAL UNIVERSITY, FURONG DISTRICT, CHANGSHA CITY, HUNAN PROVINCE |
|
| TA01 | Transfer of patent application right |
Effective date of registration: 20100803 Address after: 410128 Furong district, Hunan, Hunan Agricultural University, Changsha Applicant after: Hunan Agricultural University Address before: 410128 Institute of rice science, Hunan Agricultural University, Furong district, Hunan, Changsha Applicant before: Chen Liyun Co-applicant before: Xiao Yinghui Co-applicant before: Tang Wenbang |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20101103 Termination date: 20121210 |