CN102498997B - Method of screening nucleic sterile plants of photo-thermo-sensitive nucleic sterile rice and method of propagating core seeds - Google Patents
Method of screening nucleic sterile plants of photo-thermo-sensitive nucleic sterile rice and method of propagating core seeds Download PDFInfo
- Publication number
- CN102498997B CN102498997B CN201110356878.5A CN201110356878A CN102498997B CN 102498997 B CN102498997 B CN 102498997B CN 201110356878 A CN201110356878 A CN 201110356878A CN 102498997 B CN102498997 B CN 102498997B
- Authority
- CN
- China
- Prior art keywords
- sterile
- nucleic
- temperature
- core
- sensitive
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Disclosed are a method of screening nucleic sterile plants of photo-thermo-sensitive nucleic male sterile rice and a method of propagating nucleic seeds. The method of screening nucleic sterile plants of photo-thermo-sensitive nucleic male sterile rice includes: using artificial circulating water at the certain constant temperature within a range of from 22.5 DEG C to 23.5 DEG C to irrigate large-group photo-thermo-sensitive nucleic male sterile rice at a fertility sensitive stage; and screening sterility-stable nucleic sterile plants with zero fertile pollen. The method of propagating nucleic seeds includes: allowing nucleic sterile plants with ears cut off to regenerate and tiller into a fertility sensitive stage; and then using the artificial circulating water at the certain constant temperature within a range of from 19.5 DEG C to 21.5 DEG C to irrigate so as to lead the plants to self and seed to propagate nucleic seeds. Using the methods solves the serious problem that hybrid seed production fails due to the fact that part of sterile plants restores fertility occasionally at low temperature when the critical sterility temperature is too high in hybrid seed production. By propagating the nucleic seeds, the scale of the nucleic plants is widened to meet practical production requirements, and significance on promoting industrial development of 'two-line' hybrid rice is achieved.
Description
Technical field
This invention relates to a kind of method of screening the sterile strain of photoperiod-temperature sensitive genie male-sterile line paddy rice core and breeding core seeds.
Background technology
1981, Shi Mingsong is reported in land-reclaimable 58 colonies of paddy rice and has found a strain male sterile plant, studies have shown that this male sterile plant has dual-purpose feature, be that before September 3, heading is male sterile, can be used as male sterile line for hybrid seeding, the pollen of earing after September 3 can be educated and self-fertility, for breeding male sterile lines seed.By research called after Hubei photosensitive-sensitive sterile line Nongken 58S.Within 1987, Deng Huafeng invents temperature sensitive dual-purpose male sterile line peace agriculture S-1 under the guidance of Yuan Longping, Li Bihu.Thereby make hybrid rice develop into " two line method " by " three line method ".But in the research of " two line method " hybrid rice and application, find, the fertility of photosensitive-sensitive sterile line is temperature influence also; No matter temperature sensitive or photosensitive dual-purpose male sterile line, increases with propagation generation, and between each strain, critical sterile temperature occurs separated, partial sterility strain when hybrid seeding is because critical sterile excess Temperature, under accidental low temperature, recover fertility, cause hybrid seeding failure, cause heavy economic losses.Therefore, how to filter out critical sterile temperature low, be not subject to unexpected low temperature effect, core strain that sterility is stable and the key technology that becomes the stable development of promotion " two line method " hybrid rice with core strain breeding core seeds.
Summary of the invention
Object of the present invention has two: one is to provide a kind of method of screening the sterile strain of photoperiod-temperature sensitive genie male-sterile line paddy rice core, to solve when the hybrid seeding partial sterility strain because critical sterile excess Temperature, under accidental low temperature, recover fertility, cause the serious problems of hybrid seeding failure; Two are to provide a kind of method of breeding core seeds, for expanding the colony of core strain, meet the needs of large-scale production male-sterile seed.
The method of the sterile strain of screening photoperiod-temperature sensitive genie male-sterile line paddy rice core provided by the invention, the large group photoperiod-temperature sensitive genie male-sterile line paddy rice in fertility-sensitive period to be carried out to artificial cycle water under natural lighting condition irrigate 6～12 days, water irrigating depth is as the criterion to flood young fringe, water temperature is constant is certain value within the scope of 22.5 ℃～23.5 ℃, irrigation finishes rear recovery natural daylight temperature cultivation condition, filters out fertile pollen and be 0 the stable sterile strain of core of sterility.
The method of breeding core seeds provided by the invention, that the sterile strain of photoperiod-temperature sensitive genie male-sterile line paddy rice core filtering out is prescinded to spike of rice, divide the root and stem of certain plants to transplant, when the sterile strain of core regeneration tiller development is when starting to enter fertility-sensitive period, under natural lighting condition, carry out artificial cycle water irrigation 15～20 days, water irrigating depth is as the criterion to flood young fringe, water temperature is constant is certain value within the scope of 19.5 ℃～21.5 ℃, irrigation finishes rear recovery natural daylight temperature cultivation condition, makes it self-fertility, breeding core seeds.
The method of the sterile strain of screening photoperiod-temperature sensitive genie male-sterile line paddy rice core provided by the invention and breeding core seeds, can continue, stable filter out critical sterile temperature low, be not subject to unexpected low temperature effect, core strain that sterility is stable, and breed core seeds with this core strain.Solved partial sterility strain when hybrid seeding because critical sterile excess Temperature; under accidental low temperature, recover fertility, cause the serious problems of hybrid seeding failure, simultaneously by breeding core seeds; for expanding the colony of core strain, meet the needs of large-scale production male-sterile seed.To promoting " two line method " hybrid rice industry development tool significance.
Embodiment
Embodiment mono-
500～1000 individual plants of plantation photoperiod-temperature sensitive genie male-sterile line paddy rice P88S, when the main fringe of photoperiod-temperature sensitive genie male-sterile line Rice Population is grown while starting to enter fertility-sensitive period, with the cycle water that temperature constant is 23.5 ℃, irrigate 6～10 days, when male sterile rice heading is bloomed, the flower pesticide of getting 3 flowers from each individual plant continuous 5 day every day is placed in slide liquor kalii iodide and makes pollen staining sheet, examine under a microscope the sterile plant of superseded occurred fertile pollen.The sterile strain that fertile pollen is 0, sterility is stable is the sterile strain of core.Prescind the spike of rice of the sterile strain of core, minute root and stem of certain plants is transplanted.When regeneration tiller development is when starting to enter fertility-sensitive period, with constant 19.5 ℃～21.5 ℃ preferably water circulation irrigations 15～20 days of 21 ℃.When the sterile strain regeneration of core, tiller when heading is bloomed and divide individual plant bagging with white paper bag, during the loose powder of blooming, beat white paper bag every day, promotes that loose powder makes it self-fertility.The timely sowing of seed yellow maturity time-division individual plant, this seed is male sterile line core seeds.
Embodiment 2
500～1000 individual plants of plantation photoperiod-temperature sensitive genie male-sterile line paddy rice Peiai 64S, when the main fringe of photoperiod-temperature sensitive genie male-sterile line Rice Population is grown while starting to enter fertility-sensitive period, with the cycle water that temperature constant is 23.5 ℃, irrigate 10～12 days, when male sterile rice heading is bloomed, the flower pesticide of getting 3 flowers from each individual plant continuous 5 day every day is placed in slide liquor kalii iodide and makes pollen staining sheet, examine under a microscope the sterile plant of superseded occurred fertile pollen.The sterile strain that fertile pollen is 0, sterility is stable is the sterile strain of core.Prescind the spike of rice of the sterile strain of core, minute root and stem of certain plants is transplanted.When regeneration tiller development is when starting to enter fertility-sensitive period, with constant 21.0 ℃ of water, carry out cyclic irrigation 15～20 days.When the sterile strain regeneration of core, tiller when heading is bloomed and divide individual plant bagging with white paper bag, during the loose powder of blooming, beat white paper bag every day, promotes that loose powder makes it self-fertility.The timely sowing of seed yellow maturity time-division individual plant, this seed is male sterile line core seeds.
Embodiment 3
500～1000 individual plants of plantation photoperiod-temperature sensitive genie male-sterile line paddy rice Y58S, when the main fringe of photoperiod-temperature sensitive genie male-sterile line Rice Population is grown while starting to enter fertility-sensitive period, with the cycle water that temperature constant is 23.0 ℃, irrigate 10 days, when male sterile rice heading is bloomed, the flower pesticide of getting 3 flowers from each individual plant continuous 5 day every day is placed in slide liquor kalii iodide and makes pollen staining sheet, examine under a microscope the sterile plant of superseded occurred fertile pollen.The sterile strain that fertile pollen is 0, sterility is stable is the sterile strain of core.Prescind the spike of rice of the sterile strain of core, minute root and stem of certain plants is transplanted.When regeneration tiller development is when starting to enter fertility-sensitive period, with constant 21.0 ℃ of water, carry out cyclic irrigation 15～18 days.When the sterile strain regeneration of core, tiller when heading is bloomed and divide individual plant bagging with white paper bag, during the loose powder of blooming, beat white paper bag every day, promotes that loose powder makes it self-fertility.The timely sowing of seed yellow maturity time-division individual plant, this seed is male sterile line core seeds.
Embodiment 4
500～1000 individual plants of plantation photoperiod-temperature sensitive genie male-sterile line paddy rice strain 1S, when the main fringe of photoperiod-temperature sensitive genie male-sterile line Rice Population is grown while starting to enter fertility-sensitive period, with the cycle water that temperature constant is 22.5 ℃, irrigate 12 days, when male sterile rice heading is bloomed, the flower pesticide of getting 3 flowers from each individual plant continuous 5 day every day is placed in slide liquor kalii iodide and makes pollen staining sheet, examine under a microscope the sterile plant of superseded occurred fertile pollen.The sterile strain that fertile pollen is 0, sterility is stable is the sterile strain of core.Prescind the spike of rice of the sterile strain of core, minute root and stem of certain plants is transplanted.When regeneration tiller development is when starting to enter fertility-sensitive period, with constant 21.0 ℃ of water, carry out cyclic irrigation 16 days.When the sterile strain regeneration of core, tiller when heading is bloomed and divide individual plant bagging with white paper bag, during the loose powder of blooming, beat white paper bag every day, promotes that loose powder makes it self-fertility.The timely sowing of seed yellow maturity time-division individual plant, this seed is male sterile line core seeds.
Claims (3)
1. a method of screening the sterile strain of photoperiod-temperature sensitive genie male-sterile line paddy rice core, it is characterized in that the large group photoperiod-temperature sensitive genie male-sterile line paddy rice in fertility-sensitive period is carried out to artificial cycle water under natural lighting condition irrigates 6～12 days, water irrigating depth is as the criterion to flood young fringe, water temperature is constant is certain value, irrigation finishes rear recovery natural daylight temperature cultivation condition, filter out fertile pollen and be 0 the stable sterile strain of core of sterility, wherein P88S water temperature is constant is 23.5 ℃, Peiai 64S water temperature is constant is 23.5 ℃, Y58S water temperature is constant is 23.0 ℃, and strain 1S water temperature is constant is 22.5 ℃.
2. the method with the sterile strain of core described in claim 1 breeding core seeds, it is characterized in that the sterile strain of photoperiod-temperature sensitive genie male-sterile line paddy rice core filtering out to prescind spike of rice, divide the root and stem of certain plants to transplant, when the sterile strain of core regeneration tiller development is when starting to enter fertility-sensitive period, under natural lighting condition, carrying out artificial cycle water irrigates 15～20 days, water irrigating depth is as the criterion to flood young fringe, coolant-temperature gage constant within the scope of 19.5 ℃～21.5 ℃ certain value, irrigation finishes rear recovery natural daylight temperature cultivation condition, make it self-fertility, breeding core seeds.
3. breed according to claim 2 the method for core seeds, it is characterized in that circulating water temperature constant be 21.0 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110356878.5A CN102498997B (en) | 2011-11-11 | 2011-11-11 | Method of screening nucleic sterile plants of photo-thermo-sensitive nucleic sterile rice and method of propagating core seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110356878.5A CN102498997B (en) | 2011-11-11 | 2011-11-11 | Method of screening nucleic sterile plants of photo-thermo-sensitive nucleic sterile rice and method of propagating core seeds |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102498997A CN102498997A (en) | 2012-06-20 |
| CN102498997B true CN102498997B (en) | 2014-02-26 |
Family
ID=46211179
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110356878.5A Expired - Fee Related CN102498997B (en) | 2011-11-11 | 2011-11-11 | Method of screening nucleic sterile plants of photo-thermo-sensitive nucleic sterile rice and method of propagating core seeds |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102498997B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103891609A (en) * | 2014-03-03 | 2014-07-02 | 湖南师范大学 | Low-cadmium-in-seed rice dual-purpose sterile line screening method |
| CN105695501B (en) * | 2014-11-28 | 2020-06-19 | 上海师范大学 | Method for creating photo-thermo-sensitive sterile line and application of photo-thermo-sensitive sterile line in plant breeding |
| CN105393863A (en) * | 2015-12-02 | 2016-03-16 | 温州科技职业学院 | Paddy rice photo-thermal-sensitive sterile line generation-adding method |
| CN111602592B (en) * | 2020-05-25 | 2021-11-16 | 湖南师范大学 | Breeding method and application of rice low temperature sensitive genic male sterile line with high sterility critical temperature of high and low temperature resistance |
| CN114830936B (en) * | 2022-04-24 | 2023-07-21 | 河南师范大学 | Method for thoroughly sterility of pollen of different fertility populations of BNS series thermosensitive genic male sterile wheat |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02200130A (en) * | 1989-01-28 | 1990-08-08 | Motoji Ishida | Culture of rice plant by cold-water irrigation |
| CN1041589C (en) * | 1994-06-28 | 1999-01-13 | 湖南杂交水稻研究中心 | Low-temp.-sensitive karyosterile line of rice and seed-breeding process thereof |
| JPH09154406A (en) * | 1995-12-06 | 1997-06-17 | Takanobu Taniyama | Cultivation of paddy rice using azolla |
| JP2000191408A (en) * | 1998-12-25 | 2000-07-11 | Chisso Corp | Coated granular agrochemical, seedling bed using the same and culture of paddy rice with the seedling bed |
| CN1435082A (en) * | 2002-01-31 | 2003-08-13 | 广东省农业科学院水稻研究所 | Method for breeding temp.-sensitive caryon sterile line seed of rice |
| CN101248750B (en) * | 2007-12-10 | 2010-11-03 | 湖南农业大学 | Paddy rice dual-purpose genic male sterile line single-season with regeneration cold water string irrigating seed breeding method |
| CN101999302B (en) * | 2010-10-19 | 2011-12-28 | 湖北省农业科学院粮食作物研究所 | Breeding method of japonica photoperiod/thermo-sensitive genic male sterile lines |
| CN102119640A (en) * | 2010-11-19 | 2011-07-13 | 湖南农业大学 | Method for producing rice dual-purpose nucleic sterile line original seed |
-
2011
- 2011-11-11 CN CN201110356878.5A patent/CN102498997B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN102498997A (en) | 2012-06-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101999302B (en) | Breeding method of japonica photoperiod/thermo-sensitive genic male sterile lines | |
| CN102498997B (en) | Method of screening nucleic sterile plants of photo-thermo-sensitive nucleic sterile rice and method of propagating core seeds | |
| CN102498998B (en) | Method for purification, retainment and propagation of seeds of rice photo-thermo-sensitive male sterile line | |
| CN104429440B (en) | A kind of cuttage implantation methods of bamboo | |
| CN103749302A (en) | Induced acclimation and cultivation method for salt-tolerant bamboo reed seedlings | |
| CN107135950A (en) | A kind of breeding method of quick acquisition black fruit fructus lycii regrowth | |
| CN101637090B (en) | Method of cultivating nutrition pot seedling by tillering and transplanting large-scale sympodial bamboos | |
| CN105165340A (en) | Greenhouse strawberry planting method | |
| CN103535240A (en) | Breeding method for peach seedlings | |
| CN102017895B (en) | Method for producing potato virus A (PVA)-free seedling by utilizing variable temperature detoxification technology | |
| CN103988764B (en) | The method of the root induction of a kind of potato detoxicating plantlet in vitro and hardening | |
| CN106259208B (en) | A kind of method that tobacco plant repeatedly breeds aphidius gifuensis | |
| CN103120121B (en) | Method for determining thermo sensitive genetic male sterile line through sowing at different stages in cold water treatment period | |
| CN101124886B (en) | Hybrid seed production method using cotton sterile male line and recovery line to processing ratoon regeneration | |
| CN104381036A (en) | Method for adjusting flowering stage of shengguang chrysanthemum | |
| CN103430831B (en) | Method for obtaining apomixis flax seeds | |
| CN105340731A (en) | Photo-thermo-sensitive genic male-sterile rice creation method | |
| CN107646373A (en) | A kind of grafting cultivation method of tomato and water planting potato | |
| CN104082144A (en) | Wheat ear in-vitro culture method through wheat and corn hybridization and haploidembryo induction | |
| CN105794509A (en) | SNP chemical regulation method for increasing thermo-sensitive genic male sterile wheat BNS maturing rate | |
| CN104871963A (en) | Seed preparation method for blooming time overslip two-line rice hybridization | |
| CN104823790A (en) | Rapid juglans sigillata cutting propagation method | |
| CN103875389B (en) | A kind of four seasons honey longan cultivation method | |
| CN105532426A (en) | Method for outbreeding Mimai No.3 wheat | |
| CN106234312B (en) | A kind of save space, low cost, the aphidius gifuensis of economical and efficient for kind of a method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140226 Termination date: 20201111 |