CN103891609A - Low-cadmium-in-seed rice dual-purpose sterile line screening method - Google Patents
Low-cadmium-in-seed rice dual-purpose sterile line screening method Download PDFInfo
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- CN103891609A CN103891609A CN201410071571.4A CN201410071571A CN103891609A CN 103891609 A CN103891609 A CN 103891609A CN 201410071571 A CN201410071571 A CN 201410071571A CN 103891609 A CN103891609 A CN 103891609A
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Abstract
The invention discloses a method for screening a low-cadmium-in-seed rice dual-purpose sterile line. The method comprises the following steps of (1) measuring the content of cadmium in soil, and selecting the soil with the cadmium content being greater than 0.3mg/Kg and uniform and consistent for test; (2) planting the dual-purpose sterile line, and managing the sterile line according to the conventional fertilizer-water ratio; (3) when the dual-purpose sterile line grows to a stage that pollen mother cells are in the meiophase, irrigating water at the temperature of 19.5-21.5 DEG C to enable seedlings to be in low-temperature water, and performing continuous treatment for 10-18 days; (4) harvesting seeds of the dual-purpose sterile line according to the species or the strain, husking the seeds after the seeds are dried, and pulverizing husked seeds into rice flour; (5) measuring the cadmium content of the rice flour; (6) taking the dual-purpose sterile line of which the cadmium content of the rice flour is lower than 0.2mg/Kg as a low-cadmium-in-seed dual-purpose sterile line; and (7) taking the low-cadmium-in-seed dual-purpose sterile line as a female parent resource for preparing low-cadmium hybrid rice.
Description
Technical field
The present invention relates to a kind of method of screening the low cadmium paddy rice dual-purpose of seed male sterile line.
Background technology
Cadmium (Cadmium, Cd) is one of China's farmland the most general contaminated heavy metal.According to Ministry of Agriculture statistics, China exceedes 10% arable land and is subject to cadmium pollution (being greater than 0.3 mg/Kg), and the grain of the heavy metal pollutions such as annual cadmium is up to 1,200 ten thousand tons.Cadmium pollution enters human body by food chain, and long-term edible meeting serious harm human health, even causes " Itai-itai diseases ".
In the rice that the cadmium content of 31 batches of the sampling observations of Guangdong Province in 2013 food peace office cloth exceeds standard, have 14 batches to come from Hunan, cadmium content is not from per kilogram 0.26 mg/Kg to 0.93 mg/Kg etc.In the face of this severe situation, promoting the low cadmium rice varieties of seed is the main path that changes this passive situation.At present, the high-yield rice of spread is mainly two line method hybrid combination, and the two-line hybrid rice of therefore cultivating the low cadmium of seed is very urgent, and the important technology approach of cultivating low cadmium two-line hybrid rice is to cultivate the dual-purpose male sterile line of the low cadmium of seed.But dual-purpose male sterile line is all acarpous in the general world naturally, cannot carry out the analysis of seed cadmium content, inventing a kind of technology that makes dual-purpose male sterile line realize high yield breeding under high cadmium soil condition is the prerequisite of carrying out the dual-purpose male sterile line of the screening low cadmium of seed, with the dual-purpose male sterile line of the low cadmium of seed be the basis of carrying out low cadmium breeding of hybridized rice as maternal resource.The present invention is exactly according to this great demand, a kind of method that dual-purpose male sterile line that screens the low cadmium of seed has been invented in research.
Summary of the invention
The object of the present invention is to provide a kind of method of the dual-purpose male sterile line that screens the low cadmium of seed, by being greater than the dual-purpose male sterile line under 0.3 mg/Kg soil condition to being planted in cadmium content, carry out water at low temperature processing in 10-18 days in pollen mother cell to meiosis stage in growth, after male sterile line is solid, analyze the cadmium content of seed, filter out the dual-purpose male sterile line of the low cadmium of seed, make female parent with the dual-purpose male sterile line of the low cadmium of seed, prepare low cadmium hybrid rice.
Technical scheme provided by the invention is: a kind of method of the dual-purpose male sterile line that screens the low cadmium of seed, comprises the following steps:
(1) measure Cadmium in Soil content, guarantee that cadmium content is greater than 0.3 mg/Kg, and for examination Cadmium in Soil content uniformity;
(2) plant dual-purpose male sterile line 20 strains in cadmium pollution soil more than;
(3) Fertilization Level is executed N, P, the K chemical fertilizer without cadmium pollution routinely;
(4) irrigate the water without cadmium pollution, grow pollen mother cell to meiosis stage at described dual-purpose male sterile line, irrigate the water of 19.5-21.5 DEG C, make young fringe in water at low temperature, process continuously 10-18 days, use up into reduction division and enter the pollen development stage as the morphological index that stops water at low temperature processing to divide fringe base portion;
(5) seed that divides kind or strain to gather in the crops described dual-purpose male sterile line, seed is also shelling after drying, and the seed shelling is worn into ground rice;
(6) measure the cadmium content of described ground rice;
(7) in described ground rice, cadmium content is the dual-purpose male sterile line of the low cadmium of seed lower than the dual-purpose sterile of 0.2 mg/Kg.
The dual-purpose male sterile line of low cadmium of screening can be used as female parent, with paternal hybrid, prepares low cadmium hybrid rice.Technical scheme of the present invention is also applicable to determine that whether known dual-purpose male sterile line is low cadmium, thereby the dual-purpose male sterile line of low cadmium of determining can be used as female parent, with paternal hybrid, prepares low cadmium hybrid rice.
The technical problem that the present invention solves can be released by description below bright.So-called male sterile line is exactly can not self-fruitful paddy rice under general nature condition, solid because not having, therefore up to the present, and nobody expects screening low cadmium male sterile line.The inventor has invented high yield under artificial preparation water at low temperature condition and has bred the patent (authorizing) of dual-purpose male sterile line fertility, and further invention utilizes the combination of high cadmium soil condition and the dual-purpose male sterile line condition of breeding to realize the screening to the low cadmium rice sterile line of seed.Because male sterile line is not directly to produce paddy, be only a kind of material of crossbreeding, be by just likely cultivating the hybrid rice of low cadmium with low cadmium paternal hybrid, therefore male sterile line there is no need to determine for different cadmium pollution soils one by one.As long as to determine low cadmium resource with cadmium content higher than 0.3 mg/Kg soil just passable, the hybrid combination of allotting with low cadmium male sterile line be applicable to planting soil that what contains cadmium level just needs compare qualification (relating to the latter in another part of patent application that the applicant submits on the same day) for different cadmium pollution soils.
Embodiment
The present embodiment compares and analyzes with 5 parts of paddy rice dual-purpose male sterile lines and 1 conventional Rice, the dual-purpose male sterile line of the low cadmium of screening seed, and step is as follows:
(1) measure Cadmium in Soil content, guarantee that cadmium content is greater than 0.3 mg/Kg, and for examination Cadmium in Soil content uniformity;
(2), more than each dual-purpose male sterile line is planted 20 strains, Fertilization Level is executed N, P, the K chemical fertilizer without cadmium pollution routinely;
(3) irrigate the water without cadmium pollution, grow pollen mother cell to meiosis stage at dual-purpose male sterile line, irrigate the water of 19.5-21.5 DEG C, make young fringe in water at low temperature, process continuously 10-18 days, make male sterile line normally solid, use up into reduction division and enter the pollen development stage as the morphological index that stops water at low temperature processing to divide fringe base portion;
(4) point kind or strain are gathered in the crops the seed of dual-purpose male sterile line, and seed is also shelling after drying, and the seed shelling is worn into ground rice;
(5) adopt the standard method of the mensuration cadmium content of the national regulations such as graphite furnace method to measure the cadmium content of ground rice;
(6) ground rice cadmium content is the dual-purpose male sterile line of the low cadmium of seed lower than the dual-purpose sterile of 0.2 mg/Kg.
The results are shown in Table 1, find the cadmium content significant difference of dissimilar dual-purpose male sterile line seed, T62S, T56-2S and B13S seed cadmium content are lower than 0.2 mg/Kg, and these 3 sterile is the dual-purpose male sterile line of the low cadmium of seed, is suitable for preparing the maternal resource of low cadmium hybrid rice.。
Table 1
| Dual-purpose male sterile line | Seed cadmium content (mg/Kg) | Dual-purpose male sterile line | Seed cadmium content (mg/Kg) |
| T62S | 0．026 | B10-2S | 0．300 |
| T56-2S | 0．130 | B59S | 0．520 |
| B13S | 0．190 | No. 1, Hunan Xian in evening | 0.580 |
Claims (2)
1. a method of screening the low cadmium paddy rice dual-purpose of seed male sterile line, is characterized in that comprising the steps:
(1) measure Cadmium in Soil content, select Cadmium in Soil content to be greater than 0.3 mg/Kg, and the confession examination soil of cadmium content uniformity;
(2) in cadmium pollution soil, plant dual-purpose male sterile line;
(3) Fertilization Level is executed N, P, the K chemical fertilizer without cadmium pollution routinely;
(4) irrigate the water without cadmium pollution, grow pollen mother cell to meiosis stage at described dual-purpose male sterile line, irrigate the water of 19.5-21.5 DEG C, make young fringe in water at low temperature, process continuously 10-18 days, use up into reduction division and enter the pollen development stage as the morphological index that stops water at low temperature processing to divide fringe base portion;
(5) seed that divides kind or strain to gather in the crops described dual-purpose male sterile line, seed is also shelling after drying, and the seed shelling is worn into ground rice;
(6) measure the cadmium content of described ground rice;
(7) in described ground rice, cadmium content is the dual-purpose male sterile line of the low cadmium of seed lower than the dual-purpose sterile of 0.2 mg/Kg.
2. method according to claim 1, is characterized in that: in step (2), more than each described dual-purpose male sterile line is planted 20 strains.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410071571.4A CN103891609A (en) | 2014-03-03 | 2014-03-03 | Low-cadmium-in-seed rice dual-purpose sterile line screening method |
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| CN201410071571.4A CN103891609A (en) | 2014-03-03 | 2014-03-03 | Low-cadmium-in-seed rice dual-purpose sterile line screening method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105052641A (en) * | 2015-07-24 | 2015-11-18 | 湖南省水稻研究所 | Screening method of low-cadmium rice and method for breeding low-cadmium conventional late rice variety |
| CN105165596A (en) * | 2015-07-30 | 2015-12-23 | 中国水稻研究所 | Breeding method for rice varieties with grains in low accumulation of heavy metal cadmium |
| CN106613916A (en) * | 2017-01-11 | 2017-05-10 | 四川邡牌种业有限公司 | Three-line hybrid rice combination breeding method |
| CN108142267A (en) * | 2018-02-06 | 2018-06-12 | 湖南师范大学 | Quickly screen the sand culture method of low cadmium seed rice varieties |
| CN111837941A (en) * | 2020-08-10 | 2020-10-30 | 四川省农业科学院水稻高粱研究所 | Breeding method of rice hybrid variety with low cadmium content |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102498997A (en) * | 2011-11-11 | 2012-06-20 | 湖南师范大学 | Method of screening nucleic sterile plants of photo-thermo-sensitive nucleic male sterile rice and method of propagating nucleic seeds |
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2014
- 2014-03-03 CN CN201410071571.4A patent/CN103891609A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102498997A (en) * | 2011-11-11 | 2012-06-20 | 湖南师范大学 | Method of screening nucleic sterile plants of photo-thermo-sensitive nucleic male sterile rice and method of propagating nucleic seeds |
Non-Patent Citations (2)
| Title |
|---|
| 徐燕玲等: ""低镉累积水稻品种的筛选方法研究——品种与类型"", 《农业环境科学学报》, vol. 28, no. 7, 31 December 2009 (2009-12-31), pages 1346 - 1352 * |
| 曾翔: ""水稻镉积累和耐性机理及其品种间差异研究"", 《中国博士学位论文全文数据库 农业科技辑》, no. 4, 15 April 2008 (2008-04-15), pages 047 - 1 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105052641A (en) * | 2015-07-24 | 2015-11-18 | 湖南省水稻研究所 | Screening method of low-cadmium rice and method for breeding low-cadmium conventional late rice variety |
| CN105165596A (en) * | 2015-07-30 | 2015-12-23 | 中国水稻研究所 | Breeding method for rice varieties with grains in low accumulation of heavy metal cadmium |
| CN105165596B (en) * | 2015-07-30 | 2017-07-28 | 中国水稻研究所 | A kind of selection of the rice varieties of the low heavy metal cadmium accumulation of seed |
| CN106613916A (en) * | 2017-01-11 | 2017-05-10 | 四川邡牌种业有限公司 | Three-line hybrid rice combination breeding method |
| CN108142267A (en) * | 2018-02-06 | 2018-06-12 | 湖南师范大学 | Quickly screen the sand culture method of low cadmium seed rice varieties |
| CN111837941A (en) * | 2020-08-10 | 2020-10-30 | 四川省农业科学院水稻高粱研究所 | Breeding method of rice hybrid variety with low cadmium content |
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