CN101044834B - Method for producing hybrid rice seeds of light-temperature sensitive male sterility line by using low-temperature or short-day low temperature - Google Patents
Method for producing hybrid rice seeds of light-temperature sensitive male sterility line by using low-temperature or short-day low temperature Download PDFInfo
- Publication number
- CN101044834B CN101044834B CN2006100659457A CN200610065945A CN101044834B CN 101044834 B CN101044834 B CN 101044834B CN 2006100659457 A CN2006100659457 A CN 2006100659457A CN 200610065945 A CN200610065945 A CN 200610065945A CN 101044834 B CN101044834 B CN 101044834B
- Authority
- CN
- China
- Prior art keywords
- temperature
- sterile
- low
- sensitive
- male
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Abstract
A method for culturing the seeds of photosensitive and thermo-sensitive male sterility line of low-temp sterile or short-day low-temp sterile rice mainly includes the following steps: 1) choosing male parent and female parent and sowing, wherein the photosensitive and thermo-sensitive sterility line of low-temp sterile or short-day low-temp sterile is taken as the female parent; 2) during young panicle fertility transform sensitive stage of female parent, irrigating with cold water to lower the temperature of panicle when the ambient temperature is higher than the critical temperature of fertility transform of female parent, in order to keep the young panicle at the temperature which is lower than critical temperature of fertility transform and maintain completely male sterility; 3) hybridizing male parent and female parent; 4) growing and harvesting. The invention can resolve the problems of sterility and instability generated during culturing the seeds of photosensitive and thermo-sensitive sterility line of low-temp sterile or short-day low-temp sterile type rice, and can artificially control sterility of the rice seeds. The invention can also improve the breeding efficiency of the good combining ability photosensitive and thermo-sensitive sterility line and the various types of strong-good hybridization combination.
Description
Technical field
The present invention relates to the breeding field.Particularly, the present invention relates to the producing method for seed of the sterile or short day sterile type rice photo-thermo-sensitive of low temperature genic male sterile line of a kind of low temperature.
Background technology
Paddy rice is a self-pollinated plant, and still, people find that already the paddy rice of hybrid has the hybrid vigour that comprises many merits.Therefore, male sterile line be owing to can exempt artificial emasculation, save manpower, reduce seed costs, guarantee seed purity, thereby paid attention to widely in the breeding field.Photoperiod-temperature sensitive genie male-sterile line is a comparatively general phenomenon of occurring in nature.Photo-thermo-sensitive genic male sterile line has tangible fertility transfer characteristic, and its Fertility Expression is subjected to light, the warm factor is regulated, and fertility-sensitive period is under some temperature light condition, and patrogenesis is sterile; Fertility-sensitive period is under other some temperature light condition, and patrogenesis is for educating.Found the photoperiod-temperature sensitive genie male-sterile line of various ways such as the sterile or long day high temperature of high temperature is sterile, and the sterile or short day low temperature of low temperature is sterile at present.High temperature or length day high temperature sterile (low temperature or short day low temperature can be educated) type light temperature-sensitive sterile line is to a certain degree sterile with the high temperature performance of time in fertility conversion sensitive periods chance, so generally utilize it to carry out the production of hybrid seeds in the summer of long day high temperature; Or meet to a certain degree and can educate with the low temperature performance of time, so sterile line propagation is carried out in general using Hainan winter and high altitude localities.Low temperature or short day sterile (high temperature or long day high temperature can be educated) the type light temperature-sensitive sterile line of low temperature (some people is called anti-temperature sensitive or reflective temp-sensing sterile line) to a certain degree can be educated with the high temperature performance of time in fertility conversion sensitive periods chance, or chance is to a certain degree sterile with the low temperature performance of time, is respectively applied for the breeding and the production of hybrid seeds.In present photo-thermo-sensitive genetic male sterile line, temperature is the key factor of control Fertility Expression, does not have to find pure photosensitive sterile line by illumination control, and they mostly are the single-gene recessive cytoblast sterile.
Photo-thermo-sensitive genetic male sterile line both can show as male-fertile, carried out self-reproduction; It is sterile to show holandry again, makes female parent and carries out the production of hybrid seeds with conventional incross, accomplishes that one is dual-purpose, is the main genetic tool of present crop two line method heterosis utilization, and professors Yuan Longping in 1986 propose the strategy imagination of double-hybrid rice strains breeding.Extensively having carried out all over the world is maternal crop two line method heterosis utilization research with the photo-thermo-sensitive genetic male sterile line.Paddy rice two line method heterosis utilization has distinguishing features such as the easy transformation of photo-thermo-sensitive genetic male sterile line, combo freedom, and all conventional rice kinds can both transformation be photo-thermo-sensitive genetic male sterile line in theory, and all conventional varieties can both be as recovering system.Thereby the research has the breeding potentiality of high-quality, super high-yielding.The two-line hybrid rice breeding has now obtained very ten-strike, has entered large-area applications on producing, and they are general than the Three-line Hybrid increasing production of rice 5-15% with the ripe phase, and the two-line hybrid rice development is promising.
In the ANOMALOUS VARIATIONS of production of hybrid seeds stage light and temperature condition, can make the photo-thermo-sensitive genic male sterile line sterility occur fluctuating and recover can educate.For example, midsummer, low temperature can cause that the sterile or long day sterile type light temperature-sensitive sterile line of high temperature of the high temperature of having bred recovers self-fertility to some extent, causes production of hybrid seeds failure.Research in recent years and production practices explanation, because the natural world Changes in weather is irresistible, climatic variation difference is big between year border, fluctuation appears in photo-thermo-sensitive genic male sterile line sterility easily, cause photo-thermo-sensitive genic male sterile line to recover self-fertility, the probability of production of hybrid seeds failure is bigger, can't artificially predict and control.Because Changes in weather generally is nationwide, photo-thermo-sensitive genic male sterile line production of hybrid seeds failure may will be nationwide also, so should be reduced to as far as possible little degree to the production of hybrid seeds risk of photo-thermo-sensitive genic male sterile line in the breeding.
Under given conditions, induce photo-thermo-sensitive genetic male sterile line can educate, perhaps turn to complete sterile specified temp to be referred to as critical point temperature of fertility alteration by partly educating by complete sterile knuckle section.In the practice, by the male sterile line interval sowing, male sterile line is eared at different times, the observation fertility-sensitive period is in the fertility performance under the different ambient temperature conditions, can observe the critical temperature of male sterile line fertility conversion.Also can interiorly in the controlled environment chamber simulate extraneous natural conditions, by the phytotron treatment of different temperature, obtain the critical temperature of male sterile line fertility conversion at fertility-sensitive period.The critical temperature of fertility conversion is the important indicator of photo-thermo-sensitive genic male sterile line seed selection.In the fertility-sensitive period that ear differentiation is grown, when temperature was lower than critical male sterile temperature, the sterile type light temperature-sensitive sterile line of the sterile or short day low temperature of low temperature kept holandry sterile, can the safe production of hybrid seeds; When temperature was higher than critical temperature, fluctuation appearred in the fertility of the sterile or short day sterile type light temp-sensing sterile line of low temperature of low temperature, until recovering fully and can educating, can cause production of hybrid seeds failure.In the fertility-sensitive period that ear differentiation is grown, when temperature is lower than critical male sterile temperature, the sterile or long day sterile type light temp-sensing sterile line of high temperature of high temperature will show as male portion and can educate or can educate fully, also can cause production of hybrid seeds failure.Because in production of hybrid seeds fertility-sensitive period in season, the probability that generally meets microthermal climate is less than the probability that meets hot weather, produce at present go up use mostly be the sterile or long day sterile type light temperature-sensitive sterile line of high temperature of high temperature.Sterile or the short day sterile type light temperature-sensitive sterile line of low temperature of low temperature only suits in the lower area of the fertility-sensitive period temperature of rice young panicle differentiation and development (as the area of the height above sea level) production of hybrid seeds, should use to be very limited.So the production of hybrid seeds risk that the sterile type rice photo-thermo-sensitive of low temperature male sterile line was present in sterile or short day owing to low temperature is the sterile or long day sterile photo-thermo-sensitive genic male sterile line of high temperature greater than high temperature, also seldom is applied at present, their production of hybrid seeds safety problem needs solution.
Summary of the invention
The present invention is based on and solves the sterile or short day low temperature sterile type photo-thermo-sensitive genic male sterile rice line production of hybrid seeds safety problem of low temperature and propose and finish.
The producing method for seed that the purpose of this invention is to provide the sterile type light study on temperature sensitive male sterility of the sterile or short day low temperature of a kind of low temperature system.
Producing method for seed according to the present invention may further comprise the steps:
1) choose male parent and female parent is sowed, wherein with low temperature the sterile or short day sterile photo-thermo-sensitive genetic male sterile line of low temperature as female parent;
2) change sensitive periods in the young fringe fertility of female parent, when outside air temperature is higher than the critical temperature of maternal fertility conversion, carry out cold water irrigation, reduce panicle temperature, so that maternal young fringe is under the condition of the critical temperature that is lower than the fertility conversion, keep holandry sterile, and the temperature that can avoid the external world to be higher than the critical temperature of maternal fertility conversion cause maternal young fringe to be converted to partially or completely male-fertile;
3) make male parent and hybridization of female parent;
4) growth and results.
Preferably as wild goose farming S, 2610S low temperature is sterile or the short day sterile type light temp-sensing sterile line of low temperature can be used method of the present invention and carry out the production of hybrid seeds.
Particularly, the present invention is that the fertility inversion temperature sensitive part of the sterile or short day sterile type light temp-sensing sterile line of low temperature according to low temperature is the principle at rice strain base portion, one fertility-sensitive period that is enclosed within the sterile or short day sterile type light temp-sensing sterile line of low temperature of low temperature is provided, by the string comparatively constant cold water of temperature of pouring water, keep the regular hour and reach and make the sterile type light temp-sensing sterile line of the sterile or short day low temperature of low temperature keep sterile producing method for seed fully, and adopt supporting cultivation technique measure to improve the output and the seed purity of male sterile line hybrid seeding.The fertility-sensitive period of growing at young fringe begins (to be generally fourth grain husk the 3rd phase of method,), when outside air temperature is higher than the critical temperature of male sterile line fertility conversion, carry out cold water string irrigating, the string temperature of pouring water in field remains on (preferable to be between 18 ℃-26 ℃) under the male sterile line critical point temperature of fertility alteration, as required and weather determine cold water string irrigating fate (maintaining between 1-20 days), guarantee field children's fringe sensitive periods all under low temperature (under the critical point temperature of fertility alteration) condition, outside air temperature can not gone here and there filling cold water under critical point temperature of fertility alteration the time.When all young fringe fertility-sensitive periods finish (growth enters 7 after dates), no longer string is irritated cold water, and adopts supporting cultivation management technology measure to carry out the male sterile line hybrid seeding.
The present invention adopts supporting cultivation management technology measure mainly to comprise the following aspects:
1, when arranging production of hybrid seeds ground, fertility-sensitive period is in the easier treatment effect that reaches of weather conditions of lower temperature, and China South China is spring early, and SOUTHERN OF HAINAN winter, the middle and high Altitude Regions production of hybrid seeds is better, and other cold water string irrigatings area easily also can carry out.
2, begin sowing in good time: the sensitive periods that this class photo-thermo-sensitive genic male sterile line fertility changes, grown the 3-6 phase (Ding Yingfa) at young fringe, when arranging sowing time, generally fertility-sensitive period is arranged under the relatively mild weather conditions, the temperature of heading flowering then requires more than 22 ℃.
3, father and mother's in-group early sends out length fast, and growth is neat, and strengthens the male parent cultivation.Cultivate strong seedling, form many tillers in strong sprout, early send out by the short Honda of rich water regulation measure and tiller, urge growth fast, growth neatly so that reduce the influence of cold water string irrigating to parents'growth, and can reduce the cold water string irrigating fate between short strain.
4, the young fringe development progress of prediction carries out cold water string irrigating on time.Grow and entered for the 3rd phase when being checked through young fringe, when extraneous daily mean temperature is higher than the critical temperature of male sterile line fertility conversion, begins to go here and there and irritate temperature and be in constant cold water between 18-26 ℃.Require evenly to flow, flow is big, to make full water in field temperature roughly balanced, not influenced by too high temperature.The water temperature of delivery port is generally below 26 ℃, and the water temperature of water inlet is generally more than 18 ℃, and the lower limit water temperature can not depart from 18 ℃ too far away, in order to avoid injury from low temperature children fringe or stop young fringe and grow.When young fringe all entered for 7 phases, just no longer string was irritated cold water.The depth of water requires to flood young fringe fully.If temperature is low to moderate below the male sterile line critical point temperature of fertility alteration, can stops string and irritate cold water.If certain daily mean temperature is low to moderate below the male sterile line critical point temperature of fertility alteration, can not go here and there filling cold water the same day.
5, supplement the nutrients, promote young fringe to grow.Behind the cold water irrigation, growing slows down, and occurs blade flavescence phenomenon sometimes, in time mend and execute the compound fertilizer that contains various nutrient elements.Accomplish fluently the suitable heavy dressing of gibberellin during heading, not only can promote to create the high outcrossing rate of the production of hybrid seeds, and can make spike of rice that indivedual later stages do not experience low temperature after using gibberellin, seedlings of cereal crops excessive growth, can not ear sheath, in order to impurity elimination.
6, removal of impurities in time, indivedual spikes of rice may not experienced low temperature, when heading is bloomed, if can educate, in time dispel.Other mix biology hybrids etc. also and will dispel as early as possible.
7, gather in the crops in good time, guarantee seed quality.About the neat fringe 20-25 of female parent days, rob fine harvesting.Prevent the slow growing seedling of male sterile line, back or the little tiller of regenerating on the plant, normally grouting is solid to meet high temperature, avoids self mixing.
Advantage of the present invention and good effect are as follows.
The two line method heterosis utilization that the sterile type light temp-sensing sterile line of low temperature was a genetic tool in sterile or short day with low temperature and extensive use at present with nucleo-cytoplasmic interreaction male sterility system, maintenance line and recovery be the basis three line method relatively, mainly contain following 3 superiority:
1, male sterile line can one be dual-purpose, under cryogenic conditions, be used for the production of hybrid seeds, under hot conditions, breed self, thereby can exempt maintenance line, thereby simplified production routine.
2, combo freedom, the probability of choosing fine combination greatly surpasses three line method.
3, sterile cell verify heterotic negative effect and cytoplasmic unification have been avoided.
Super hybridized rice on producing at present belongs to the two line method combination more, owing to improved the combo degree of freedom, thus improved paddy rice cross breeding advantage level.But utilizing more at present is the sterile or long day sterile photo-thermo-sensitive genic male sterile line of high temperature of high temperature.There is the sterility instability problem in they, the ear differentiation developmental stage meets microthermal climate in production of hybrid seeds season, the conversion of generation fertility, cause self-pollination solid and reduce hybrid seed purity easily, usually cause large tracts of land production of hybrid seeds failure, seriously restrict the healthy and rapid development of two-line hybrid rice, improved the most critical issue that the sterile stability of male sterile line is the two-line hybrid rice development.
Usually, the critical point temperature of fertility alteration of the sterile or short day sterile type rice photo-thermo-sensitive of low temperature male sterile line of various low temperature is between 27-30 ℃, and it shows as complete male row sterility when being lower than critical point temperature of fertility alteration.Because in production of hybrid seeds season; the low temperature sterile type rice photo-thermo-sensitive of low temperature male sterile line ear differentiation in sterile or short day developmental stage meets the hot weather of temperature on critical point temperature of fertility alteration easily; can cause male sterile line to produce normal pollen and self-fertility, so the sterile or short day sterile type rice photo-thermo-sensitive of low temperature male sterile line of low temperature also seldom used at present.Utilize the sterile type light temp-sensing sterile line of the sterile or short day low temperature of low temperature cold water string irrigating seeding technique can solve the sterility stability problem of the sterile or short day sterile type rice photo-thermo-sensitive of low temperature male sterile line of low temperature, its sterility is carried out artificial control.Production of hybrid seeds risk becomes very little, and hybrid seed yield is reliable and stable, is comparatively desirable two line method heterosis utilization mode.
Under field conditions (factors), the sterile or short day sterile type rice photo-thermo-sensitive of the low temperature sterile lines seed making of low temperature that generally has only critical point temperature of fertility alteration to reach more than 30 ℃ could be safer, but almost do not have this good male sterile line at present, and its seed selection difficulty is very big.And employing the present invention, by the cold water string irrigating seeding technique, 26 ℃ of critical point temperature of fertility alteration and the above sterile or short day sterile type rice photo-thermo-sensitive of low temperature male sterile line of low temperature thereof all can utilize.In the breeding practice, the critical point temperature of fertility alteration of the sterile or short day sterile type rice photo-thermo-sensitive of low temperature male sterile line of most of low temperature can be up to more than 27 ℃, so because the present invention arranged, the seed selection of the sterile or short day sterile type rice photo-thermo-sensitive of low temperature male sterile line of the low temperature efficient that will become is very high.Acquired character is good easily, and coordinate force is good, and the sterile or short day sterile type rice photo-thermo-sensitive of low temperature male sterile line of all types of low temperature that outcrossing rate is high obtains all types of strong advantage hybrid rice combinations easily.
At present, China early rice, japonica rice area account for more than 40% of rice growing area, because male sterile line heredity is single, lack the Japonica Hybrid combination of high yield and high quality productions in and be fit to ripe combination in morning of Yangtze river basin work pair season early rice, late rice output then presents the situation of pacing up and down in the hybridization.Utilize the present invention to help cultivating strong advantage hybrid early, Japonica Hybrid, can more easily solve Japonica Hybrid and hybrid early problem.Help hybrid rice and develop by leaps and bounds again, form the hybrid rice technology that China has independent intellectual property right, keep the leading position of China, play a significant role hybrid rice.Its extensive use may make the crop heterosis utilization more universal, brings into play significant role in the foundation of Food Security from now on.
Embodiment
Embodiment 1, the sterile type caryon sterile line of low temperature wild goose farming S and male parent 9311 hybrid seedings
Maternal: wild goose farming S (Hunan product careful calendar year 2001 No. 313; Hybrid rice 1998,13 (4): 37-38), breed the sterile type photo-thermo-sensitive genetic male sterile line of authorizing by the Hunan Province of low temperature for Hengyang City, the Hunan institute of agricultural sciences.28-29 ℃ of wild goose farming S critical point temperature of fertility alteration.
Male parent: breeding of hybrid rice is used male parent 9311 always, and (state examines rice 2001001 to extensive use two-line rice hybrids two line system in the production; Jiangsu agricultural journal, 2000,16 (4): male parent 193～196).
Determine production of hybrid seeds base.Select pouring water under the big-and-middle-sized reservoir in Changsha be easily sleeve configuration sunny, damage by disease and insect is few, the large tracts of land paddy field in blocks of fertile soil, traffic convenience, requirement can guarantee cold feed, in 100 meters, except that male parent, there is not other rice varieties around the seed farm.
Select best heading flowering period.Daily mean temperature 24-30 ℃, when flower 28-32 ℃ of temperature, relative moisture 70%-90% does not have continuous three days of rain daytime, and is calm or gentle breeze arranged.Preferable with August 10 to August 20.
By leaf age difference phase predication method, time difference predication method and temperature difference predication method are calculated this sowing of father and mother difference phase accurately.Male parent 9311 generally should be than wild goose farming S early sowing 23-25 days in heading on August 10 to August 20.The general time broadcast 9311 May 8, broadcast wild goose farming S on June 2.
Cultivate the strong seedling of of the right age many tillers.Before the seed soaking seed is tedded 1～2 day.The screening seed is removed the germination grain that mixes in seed, clod, infected seed etc.Earlier with sorting seed by salt water, carry out presoaking and germinating again after cleaning with clear water then during seed soaking.Father and mother's vernalization originally requires to accomplish " fast, even, strong ".Choose the rice seedling bed, following vola fertilizer, light seeding is even broadcasts plastic mulching.Grow to two leaves heavy dressing tillering fertilizer wholeheartedly the time, transplant to execute to send in preceding 5～7 days and transfer fertilizer.A leaf wholeheartedly to two leaves wholeheartedly during per hectare spray 750 kilograms of promotions of paclobutrazol solution of 2/10000ths. five and tiller.
Basic seedling is participated in rational close planting.Father and mother's one's own profession is than 2: 12,13～20 centimetres of the spacing in the rows of male parent, 33～40 centimetres of line-spacings; 13～17 centimetres of maternal spacing in the rows, 13～17 centimetres of line-spacings.Father and mother originally all are advisable to insert two paddy seedlings.
Enhance field management, put up high yield seedling frame, strengthen the male parent cultivation.Suitable nitrogen, high phosphorus, potassium cultivation, the ratio of nitrogen, phosphorus, potassium is about 1: 1: 1.5.Male parent is carried out fertile partially management, and male parent was transplanted the back 4～6 days, and per hectare is executed 45～60 kilograms in urea, maternally transplanted the back 4～6 days, and per hectare imposes 150 kilograms in urea, promoted early to send out steady long, reached big panicle many grains per panicle, total grain husk and how spent and the big purpose of pollen amount.Male parent is looked the growth of cereal crop seedlings and is executed ball fertilizer partially one time before late tillering state ear differentiation.Total requirement is thin water cutting seedling on the water starch management, open field behind the root and stem of certain plants of living, and shallow water is diligent irritates, alternation of wetting and drying,, meta joint low to promote tillered.Can be in conjunction with intertill and clean tillage, see that seedling sees weather with seeing, the draining open field to strengthen the oxygen in the soil, inspires new root moderately and at the right moment, promotes that the seedlings of cereal crops steadily and surely grow.To do the prediction of disease worm simultaneously well, early control.Attention control rice fulgorid, snout moth's larva, line are withered, bacterial leaf spot, rice blast etc.
Predict young fringe development progress, carry out cold water string irrigating on time.Grow when entering for the 3rd phase when being checked through maternal young fringe, daily mean temperature is higher than 27 ℃, begins to go here and there the constant cold water of irritating 19-24 ℃.Require evenly to flow, flow is big, and the water at low temperature water layer floods young fringe at least.Want to make full water in field temperature roughly balanced, not influenced by too high temperature, the local everywhere water temperature of seed farm is not higher than 26 ℃.The water temperature of delivery port is below 26 ℃, and the water temperature of water inlet is more than 18 ℃, and the lower limit water temperature can not depart from 18 ℃ too far away, in order to avoid injury from low temperature children fringe or stop young fringe and grow.Wild goose farming S critical point temperature of fertility alteration is higher than 27 ℃, if the same day, certain period temperature was low to moderate below 27 ℃, can stop cold water string irrigating.If certain daily mean temperature is low to moderate below 27 ℃, can not go here and there filling cold water the same day.String is irritated low-temperature cold water after a couple of days, all grows when entering for 7 phases when young fringe, stops string and irritates.
Supplement the nutrients, promote young fringe to grow.Behind the cold water irrigation, growing slows down, and occurs blade flavescence phenomenon sometimes, in time mend and execute the compound fertilizer that contains various nutrient elements.
Adopt aggregate measures, improve maternal outcrossing seed-setting rate.Science is used " gibberellin.Make the elongation of maternal ear stem, increase maternal sword-like leave and stem angle, remove maternal " bag neck ", improve fringe grain exposing ratio, increase grain husk the flowers are in blossom clever angle, prolong and close the clever time, improve maternal stigma exposing ratio.Ear 5 percent to 10 at female parent, per hectare is sprayed father and mother originally simultaneously with gibberellin 30 grams; Maternal heading 30 20 percent to percent o'clock, per hectare is maternal with gibberellin 120 gram sprays; Maternal heading 50 percent o'clock, per hectare is sprayed father and mother originally simultaneously with gibberellin 75 grams.Adopt the manually auxiliary powder of catching up with of bamboo bar.
In time prescind assorted strain.Indivedual spikes of rice may not experienced low temperature, can show and can educate, and when heading is bloomed, in time dispel.Simultaneously at whole growing stage, assorted strain that repeated multiple times will sneak into thoroughly, pull out neatly.
Later stage diseases and pests controlling, water and fertilizer management etc. can be with reference to the management methods of the series of three-series hybrid rice production of hybrid seeds, but want the attention control rice kernel smut especially.
Results are guaranteed seed quality in good time.About 25 days, rob fine harvesting at the neat fringe of female parent, make indivedual later stages not experience the seedling of low temperature, regrowth, tillering for a short time, it is solid normally to be in the milk.
Production of hybrid seeds result of study shows, handles through cold water string irrigating, and wild goose farming s performance holandry is sterile, and flower pesticide does not ftracture, no self-fertility, and hybrid seed purity reaches 98.7%, and hybrid seed yield is up to 2820 kilograms/hectare simultaneously.The concurrent control wild goose farming S self-fruitful rate of handling without cold water string irrigating reaches 42.9%, can not be used for the production of hybrid seeds.
The sterile type caryon sterile line of the embodiment 2 low temperature wild goose farming S and extensive 299 production of hybrid seeds in male parent Hunan
Maternal: wild goose farming S (Hunan product careful calendar year 2001 No. 313; Hybrid rice 1998,13 (4): 37-38), breed the sterile type photo-thermo-sensitive genetic male sterile line of authorizing by the Hunan Province of low temperature for Hengyang City, the Hunan institute of agricultural sciences.28-29 ℃ of wild goose farming S critical point temperature of fertility alteration.
Male parent: extensive 299 (the new variety of agricultural plant protection notification number CNA000930E) in Hunan, the series of three-series hybrid rice system of recovering commonly used.The male parent of the hybrid rice of extensive use combination Feng Yuan excellent 299 (rice 2004011 is examined in Hunan) in the production.
Determine production of hybrid seeds base.Select pouring water under the big-and-middle-sized reservoir in Changsha be easily sleeve configuration sunny, damage by disease and insect is few, the large tracts of land paddy field in blocks of fertile soil, traffic convenience, requirement can guarantee cold feed, around the seed farm in 100 meters, except that male parent, there is not other rice varieties
Select best heading flowering period.Preferable with June 28 to July 6.General Hunan extensive 299 should be than wild goose farming S early sowing 5-7 days.The general time was broadcast wild goose farming S April 1, broadcast Hunan extensive 299 on March 25.
Cultivate the strong seedling of of the right age many tillers.Seed soaking fore worker seed selection is removed germination grain, clod, infected seed etc.Earlier with sorting seed by salt water, carry out presoaking and germinating again after cleaning with clear water then during seed soaking." fast, even, strong " accomplished in father and mother's vernalization originally.Choose the rice seedling bed, following vola fertilizer, light seeding is even broadcasts plastic mulching.Male parent rice seedling bed seeding quantity is the 25-35 kg/ha, and is maternal about 45 kg/ha.Grow to two leaves heavy dressing tillering fertilizer wholeheartedly the time, transplant to execute to send in preceding 5～7 days and transfer fertilizer.A leaf wholeheartedly to two leaves wholeheartedly during per hectare spray 750 kilograms of promotions of paclobutrazol solution of 2/10000ths. five and tiller.
Basic seedling is participated in rational close planting.Father and mother's one's own profession is than 2: 12,16～20 centimetres of the spacing in the rows of male parent, 33～36 centimetres of line-spacings; 10～13 centimetres of maternal spacing in the rows, 13～17 centimetres of line-spacings.Father and mother originally all insert two paddy seedlings.
Enhance field management, put up high yield seedling frame, strengthen the male parent cultivation.Suitable nitrogen, high phosphorus, potassium cultivation, the ratio of nitrogen, phosphorus, potassium is about 1: 1: 1.5.Male parent is carried out fertile partially management, and male parent was transplanted the back 4～6 days, and per hectare is executed 45～60 kilograms in urea, maternally transplanted the back 4～6 days, and per hectare imposes 150 kilograms in urea, promoted early to send out steady long, reached big panicle many grains per panicle, total grain husk and how spent and the big purpose of pollen amount.Male parent is looked the growth of cereal crop seedlings and is executed ball fertilizer partially one time before late tillering state ear differentiation.Total requirement is thin water cutting seedling on the water starch management, open field behind the root and stem of certain plants of living, and shallow water is diligent irritates, alternation of wetting and drying,, meta joint low to promote tillered.Can be in conjunction with intertill and clean tillage, see that seedling sees weather with seeing, the draining open field to strengthen the oxygen in the soil, inspires new root moderately and at the right moment, promotes that the seedlings of cereal crops steadily and surely grow.To do the prediction of disease worm simultaneously well, early control.Attention control rice fulgorid, snout moth's larva, line are withered, bacterial leaf spot, rice blast etc.
Predict young fringe development progress, carry out cold water string irrigating on time.Grow when entering for the 3rd phase when being checked through maternal young fringe, daily mean temperature is higher than 27 ℃, begins to go here and there the constant cold water of irritating 19-24 ℃.Require evenly to flow, flow is big, and the water at low temperature water layer floods young fringe at least.Want to make full water in field temperature roughly balanced, not influenced by too high temperature, the local everywhere water temperature of seed farm is not higher than 26 ℃.The water temperature of delivery port is below 26 ℃, and the water temperature of water inlet is more than 18 ℃, and the lower limit water temperature can not depart from 18 ℃ too far away, in order to avoid injury from low temperature children fringe or stop young fringe and grow.Wild goose farming S critical point temperature of fertility alteration is higher than 27 ℃, if the same day, certain period temperature was low to moderate below 27 ℃, can stop cold water string irrigating.If certain daily mean temperature is low to moderate below 27 ℃, can not go here and there filling cold water the same day.String is irritated low-temperature cold water after a couple of days, all grows when entering for 7 phases when young fringe, stops string and irritates.
Supplement the nutrients, promote young fringe to grow.Behind the cold water irrigation, growing slows down, and occurs blade flavescence phenomenon sometimes, in time mend and execute the compound fertilizer that contains various nutrient elements.
Adopt aggregate measures, improve maternal outcrossing seed-setting rate.Science is used gibberellin.Make the elongation of maternal ear stem, increase maternal sword-like leave and stem angle, remove maternal " bag neck ", improve fringe grain exposing ratio, increase grain husk the flowers are in blossom clever angle, prolong and close the clever time, improve maternal stigma exposing ratio.Ear 5 percent to 10 at female parent, per hectare is sprayed father and mother originally simultaneously with gibberellin 30 grams; Maternal heading 30 20 percent to percent o'clock, per hectare is maternal with gibberellin 120 gram sprays; Maternal heading 50 percent o'clock, per hectare is sprayed father and mother originally simultaneously with gibberellin 75 grams.Adopt the manually auxiliary powder of catching up with of bamboo bar.
In time prescind assorted strain.Indivedual spikes of rice may not experienced low temperature, can show and can educate, and when heading is bloomed, in time dispel.Simultaneously at whole growing stage, assorted strain that repeated multiple times will sneak into thoroughly, pull out neatly.
Later stage diseases and pests controlling, water and fertilizer management etc. can be with reference to the management methods of the series of three-series hybrid rice production of hybrid seeds, but want the attention control rice kernel smut especially.
Results are guaranteed seed quality in good time.About 25 days, rob fine harvesting at the neat fringe of female parent, make indivedual later stages not experience the seedling of low temperature, regrowth, tillering for a short time, it is solid normally to be in the milk.
Production of hybrid seeds result of study shows, handles through cold water string irrigating, and wild goose farming S performance holandry is sterile, and flower pesticide does not ftracture, no self-fertility, and hybrid seed purity reaches 98.4%, and hybrid seed yield is up to 2612 kilograms/hectare simultaneously.The contrast wild goose farming S self-fruitful rate of handling without cold water string irrigating reaches 21.3%.
Embodiment 3 low temperature sterile type caryon sterile line 2610S and male parent 9311 hybrid seedings
Maternal: 2610S, the sterile type photo-thermo-sensitive genetic male sterile line of in fragrant 125S, finding for Sun Mei unit of Hunan Research Centre for Hybrid Rice of low temperature (commercially available getting).28-29 ℃ of 2610S critical point temperature of fertility alteration.
Male parent: breeding of hybrid rice is used male parent 9311 always, and (state examines rice 2001001 to extensive use two-line rice hybrids two line system in the production; Jiangsu agricultural journal, 2000,16 (4): male parent 193～196).
Determine production of hybrid seeds base.Select that the big-and-middle-sized reservoir in Changsha pouring water down is sunny easily, damage by disease and insect is few, the large tracts of land paddy field in blocks of fertile soil, traffic convenience, requirement can guarantee cold feed, seed farm in 100 meters, except that male parent, does not have other rice varieties on every side,
Select best heading flowering period.Daily mean temperature 24-30 ℃, when flower 28-32 ℃ of temperature, relative moisture 70%-90% does not have continuous three days of rain daytime, and is calm or gentle breeze arranged.Preferable with August 10 to August 20.
By leaf age difference phase predication method, time difference predication method and temperature difference predication method are calculated this sowing of father and mother difference phase accurately.9311 in heading on August 10 to August 20, generally should be than 2610S early sowing 35-37 days, and the general time broadcast 9311 about May 7, broadcast 2610S about June 12.
Cultivate the strong seedling of of the right age many tillers.Before the seed soaking seed is tedded 1～2 day.The screening seed is removed the germination grain that mixes in seed, clod, infected seed etc.Earlier with sorting seed by salt water, carry out presoaking and germinating again after cleaning with clear water then during seed soaking.Father and mother's vernalization originally requires to accomplish " fast, even, strong ".Choose the rice seedling bed, following vola fertilizer, light seeding is even to be broadcast.Grow to two leaves heavy dressing tillering fertilizer wholeheartedly the time, transplant to execute to send in preceding 5～7 days and transfer fertilizer.A leaf wholeheartedly to two leaves wholeheartedly during per hectare spray 750 kilograms of 2/10000ths. five paclobutrazol solution, tiller promoting.
Basic seedling is participated in rational close planting.Father and mother's one's own profession is than 2: 14,13～20 centimetres of the spacing in the rows of male parent, 33～40 centimetres of line-spacings; 10～13 centimetres of maternal spacing in the rows, 13～17 centimetres of line-spacings.Father and mother originally all are advisable to insert two paddy seedlings.
Enhance field management, put up high yield seedling frame, strengthen the male parent cultivation.Suitable nitrogen, high phosphorus, potassium cultivation, the ratio of nitrogen, phosphorus, potassium is about 1: 1: 1.5.Male parent is carried out fertile partially management, and male parent was transplanted the back 4～6 days, and per hectare is executed 45～60 kilograms in urea, maternally transplanted the back 4～6 days, and per hectare imposes 180 kilograms in urea, promoted early to send out steady long, reached big panicle many grains per panicle, total grain husk and how spent and the big purpose of pollen amount.Male parent is looked the growth of cereal crop seedlings and is executed ball fertilizer partially one time before late tillering state ear differentiation.Total requirement is thin water cutting seedling on the water starch management, open field behind the root and stem of certain plants of living, and shallow water is diligent irritates, alternation of wetting and drying,, meta joint low to promote tillered.Can be in conjunction with intertill and clean tillage, see that seedling sees weather with seeing, the draining open field to strengthen the oxygen in the soil, inspires new root moderately and at the right moment, promotes that the seedlings of cereal crops steadily and surely grow.To do the prediction of disease worm simultaneously well, early control.Attention control rice fulgorid, snout moth's larva, line are withered, bacterial leaf spot, rice blast etc.
Predict young fringe development progress, carry out cold water string irrigating on time.Grow when entering for the 3rd phase when being checked through maternal young fringe, daily mean temperature is higher than 27 ℃, begins to go here and there the constant cold water of irritating 19-24 ℃.Require evenly to flow, flow is big, and the water at low temperature water layer floods young fringe at least.Want to make full water in field temperature roughly balanced, not influenced by too high temperature, the local everywhere water temperature of seed farm is not higher than 26 ℃.The water temperature of delivery port is below 26 ℃, and the water temperature of water inlet is more than 18 ℃, and the lower limit water temperature can not depart from 18 ℃ too far away, in order to avoid injury from low temperature children fringe or stop young fringe and grow.The 2610S critical point temperature of fertility alteration is higher than 27 ℃, if the same day, certain period temperature was low to moderate below 27 ℃, can stop cold water string irrigating.If certain daily mean temperature is low to moderate below 27 ℃, can not go here and there filling cold water the same day.String is irritated low-temperature cold water after a couple of days, all grows when entering for 7 phases when young fringe, stops string and irritates.
Supplement the nutrients, promote young fringe to grow.Behind the cold water irrigation, growing slows down, and occurs blade flavescence phenomenon sometimes, in time mend and execute the compound fertilizer that contains various nutrient elements.
Adopt aggregate measures, improve maternal outcrossing seed-setting rate.Science is used " 920 ".Make the elongation of maternal ear stem, increase maternal sword-like leave and stem angle, remove maternal " bag neck ", improve fringe grain exposing ratio, increase grain husk the flowers are in blossom clever angle, prolong and close the clever time, improve maternal stigma exposing ratio.Ear 5 percent to 10 at female parent, per hectare is sprayed father and mother originally simultaneously with " 920 " 30 grams; Maternal heading 30 20 percent to percent o'clock, per hectare is maternal with " 920 " 120 gram sprays; Maternal heading 50 percent o'clock, per hectare is sprayed father and mother originally simultaneously with " 920 " 75 grams.Adopt the manually auxiliary powder of catching up with of bamboo bar.
In time prescind assorted strain.Indivedual spikes of rice may not experienced low temperature, can show and can educate, and when heading is bloomed, in time dispel.At whole growing stage, assorted strain that repeated multiple times will sneak into thoroughly, pull out neatly.
Later stage diseases and pests controlling, water and fertilizer management etc. can be with reference to the management methods of the series of three-series hybrid rice production of hybrid seeds, but want the attention control rice kernel smut especially.
Results are guaranteed seed quality in good time.About 25 days, rob fine harvesting at the neat fringe of female parent, make indivedual later stages not experience the seedling of low temperature, regrowth, tillering for a short time, it is solid normally to be in the milk.
Production of hybrid seeds result of study shows, handles through cold water string irrigating, and 2610S performance holandry is sterile, and flower pesticide does not ftracture, no self-fertility, and hybrid seed purity reaches 99.2%, and hybrid seed yield is up to 2406 kilograms/hectare simultaneously.The concurrent control 2610S self-fruitful rate of handling without cold water string irrigating reaches 46.7%, can not be used for the production of hybrid seeds.
Claims (2)
1. the producing method for seed of sterile or short day low temperature male sterile rice photoperiod-temperature sensitive male sterility system of a low temperature is characterized in that, said method comprising the steps of:
1) choose male parent and female parent is sowed, wherein with low temperature the sterile or short day sterile photo-thermo-sensitive genetic male sterile line of low temperature as female parent;
2) change sensitive periods in the young fringe fertility of female parent, entered for the 3rd phase when being checked through maternal young fringe growth, when extraneous daily mean temperature is higher than the critical temperature of male sterile line fertility conversion, begin to go here and there and irritate temperature and be in constant cold water between 18-26 ℃, require evenly to flow, flow is big, make full water in field temperature balanced, not influenced by too high temperature, the water temperature of delivery port is below 26 ℃, and the water temperature of water inlet is more than 18 ℃, when young fringe all entered for 7 phases, no longer string is irritated cold water, the depth of water requires to flood young fringe fully, if temperature is low to moderate below the male sterile line critical point temperature of fertility alteration, stops string and irritates cold water, if certain daily mean temperature is low to moderate below the male sterile line critical point temperature of fertility alteration, do not go here and there filling cold water the same day;
3) make male parent and hybridization of female parent;
4) growth and results.
2. the method for claim 1 is characterized in that, is suitable for using the sterile or short day sterile photo-thermo-sensitive genetic male sterile line of low temperature of low temperature of this method production of hybrid seeds to be wild goose farming S.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006100659457A CN101044834B (en) | 2006-03-27 | 2006-03-27 | Method for producing hybrid rice seeds of light-temperature sensitive male sterility line by using low-temperature or short-day low temperature |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006100659457A CN101044834B (en) | 2006-03-27 | 2006-03-27 | Method for producing hybrid rice seeds of light-temperature sensitive male sterility line by using low-temperature or short-day low temperature |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101044834A CN101044834A (en) | 2007-10-03 |
| CN101044834B true CN101044834B (en) | 2011-04-06 |
Family
ID=38770010
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2006100659457A Expired - Fee Related CN101044834B (en) | 2006-03-27 | 2006-03-27 | Method for producing hybrid rice seeds of light-temperature sensitive male sterility line by using low-temperature or short-day low temperature |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101044834B (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102668969A (en) * | 2012-01-15 | 2012-09-19 | 河南科技大学 | Breeding method for high-temperature sterile type wheat photo-thermo sensitive genic male sterile line |
| CN102972285B (en) * | 2012-12-05 | 2014-03-26 | 湖南杂交水稻研究中心 | Breeding method of hybrid crops |
| CN104170719A (en) * | 2013-12-19 | 2014-12-03 | 国家粳稻工程技术研究中心 | Method for safely breeding japonica rice light and temperature sensitive genic sterile line L39S by using Hainan climate conditions |
| CN104186306A (en) * | 2013-12-19 | 2014-12-10 | 国家粳稻工程技术研究中心 | Japonica photoperiod-temperature sensitive genetic male sterile line L62S breeding technology by utilization of string irrigation with cold water and special climate in Hainan |
| CN104186302A (en) * | 2013-12-19 | 2014-12-10 | 国家粳稻工程技术研究中心 | Technical method for multi-genotype heterosis utilization by utilizing photoperiod non-sensitive genetic male-sterile line |
| CN104170727A (en) * | 2013-12-19 | 2014-12-03 | 国家粳稻工程技术研究中心 | Method for safely breeding light and temperature sensitive genic sterile line by using Hainan climate conditions |
| CN104542267A (en) * | 2014-12-30 | 2015-04-29 | 天津天隆农业科技有限公司 | Method for reducing critical temperature of fertility alteration of japonica photo-thermo sensitive genic male sterile line L6S by utilizing conditions of high altitude and special climate of Hainan |
| CN106332770A (en) * | 2015-12-29 | 2017-01-18 | 湖南杂交水稻研究中心 | Cold region hybrid japonica rice breeding method |
| CN106857231A (en) * | 2017-03-01 | 2017-06-20 | 湖南杂交水稻研究中心 | A kind of breeding method of utilization paddy rice F2 community superiorities |
| CN110663543A (en) * | 2019-10-29 | 2020-01-10 | 甘肃农业大学 | Breeding method of temperature-sensitive dual-purpose sterile line of strong winter cabbage type winter rape |
| CN111602592B (en) * | 2020-05-25 | 2021-11-16 | 湖南师范大学 | Breeding method and application of rice low temperature sensitive genic male sterile line with high sterility critical temperature of high and low temperature resistance |
-
2006
- 2006-03-27 CN CN2006100659457A patent/CN101044834B/en not_active Expired - Fee Related
Non-Patent Citations (4)
| Title |
|---|
| 张玉烛等.两系杂交晚籼新组合雁两优921.杂交水稻17 1.2002,17(1),56. |
| 张玉烛等.两系杂交晚籼新组合雁两优921.杂交水稻17 1.2002,17(1),56. * |
| 蔡义东等.反温敏核不育水稻Go543S育性敏感部位.湖南农业大学学报(自然科学版)30 4.2004,30(4),310-311. |
| 蔡义东等.反温敏核不育水稻Go543S育性敏感部位.湖南农业大学学报(自然科学版)30 4.2004,30(4),310-311. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101044834A (en) | 2007-10-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101044834B (en) | Method for producing hybrid rice seeds of light-temperature sensitive male sterility line by using low-temperature or short-day low temperature | |
| CN107624573A (en) | A kind of cultural method of high yield and high quality rice | |
| CN103535270B (en) | Remove No. 7 wheat hybridizing breedings of wheat and propagation method | |
| CN101044835B (en) | Method for producing hybrid rice seeds of light-temperature sensitive male sterility line under higher temperature or long-day high temperature | |
| WO2014190756A1 (en) | Rice ridge-type plant-hole position-setting cultivation method | |
| CN107896798A (en) | A kind of cultural method of winter jujube | |
| CN104160949B (en) | A kind of hybrid rice opens the selection of excellent No. 18 | |
| CN100403881C (en) | Forced double cropping cultivation of fresh Jingwei jujube | |
| CN108668808A (en) | A kind of method of rice cropping | |
| CN101124886B (en) | Hybrid seed production method using cotton sterile male line and recovery line to processing ratoon regeneration | |
| CN103782896B (en) | One utilizes greenhouse by solar heat sword lily breeding method | |
| CN108293769A (en) | A kind of Protectorate cultivation method of green precious small pumpkin | |
| CN104839009B (en) | A kind of method for improving white turnip breeding quality | |
| CN104303765B (en) | The high-yield planting method of the stem of noble dendrobium | |
| CN101044833A (en) | Method for reproducing and production of hybrid seeds of rice karyoplasmic interaction type incomplete male sterility line | |
| CN107493930A (en) | A kind of autumn culture method of green precious small pumpkin | |
| CN108293770A (en) | A kind of open field culture method of green precious small pumpkin | |
| CN1094562A (en) | Method for cultivation of overyear regrowing glutinous rice | |
| CN107853171A (en) | One kind food leaf grass and its implantation methods | |
| CN109566403B (en) | Method for producing seeds by hybridizing pumpkins in two seasons in one year | |
| CN1054260C (en) | Ternary hybrid rape seed breeding technique inhibiting micropollen | |
| CN105284590A (en) | Breeding method of early-maturing girdled loofah gourd | |
| CN104221841A (en) | Chinese cabbage selfing breeding method | |
| CN110050658A (en) | A kind of thick-skinned melon, late water water chestnut rotation cropping method | |
| CN103609430A (en) | Method for selecting and reproducing early-flowering late japonica rice sterile line Zhe japonica rice 4A with high stigma exposure rate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110406 Termination date: 20130327 |