CN104542267A - Method for reducing critical temperature of fertility alteration of japonica photo-thermo sensitive genic male sterile line L6S by utilizing conditions of high altitude and special climate of Hainan - Google Patents
Method for reducing critical temperature of fertility alteration of japonica photo-thermo sensitive genic male sterile line L6S by utilizing conditions of high altitude and special climate of Hainan Download PDFInfo
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- CN104542267A CN104542267A CN201410837706.3A CN201410837706A CN104542267A CN 104542267 A CN104542267 A CN 104542267A CN 201410837706 A CN201410837706 A CN 201410837706A CN 104542267 A CN104542267 A CN 104542267A
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Abstract
The invention applies to reduction of the critical temperature of fertility alteration of a japonica photo-thermo sensitive genic male sterile line L6S by utilizing conditions of high altitude and special climate of Hainan, provides a breeding method applicable to a japonica rice thermoperiod-sensitive genie male sterile line, and belongs to the technical field of sterile line propagation. The method comprises technical steps as follows: 1, sowing and transplanting are performed at different altitudes and different stages in season in Baoshan, and a single plant with better sterile degree is selected and harvested and is brought to Hainan in October and November; 2, transplanting is performed in Hainan by stages, and the best grain-filling stage is determined; 3, fertility evaluation is performed on plant planted in season in Baoshan; 4, if the group sterility is better, the grain-filling stage in the step 2 is the best, seeds subjected to refrigerated storage can be planted at the sowing stage next year in Hainan, the seeds grow under the strict isolation conditions and are harvested in time, and seed production and application are performed in the adaptive area; 5, if the fertility is unstable, single plant with better sterility is selected, is subjected to root cutting and then is brought to Hainan for continuous transplanting by stages, the best grain-filling stage is determined, and step 2-step 4 are repeated.
Description
Technical field
The invention belongs to Japonica rice photo-thermo-sensitive genic male sterile line propagation technique field.Namely a kind of high temperature-induced sterility, low temperature can educate or long day high temperature-induced sterility, short day the propagation method of Japonica rice photo-thermo-sensitive genic male sterile line seed that can educate of low temperature.
Background technology
Two be caryon sterile line also known as photo-thermo-sensitive genic male sterile line, its Sterility variation controls by booting light and temperature condition in period.Generally under high temperature (> about 23 DEG C), long-day (> about 13.5h) condition, show as pollen abortion, carry out hybrid seeding as male sterile line with this understanding.And under low temperature short-day condition, fertility can be restored to a certain extent again and carry out self-reproduction.The development of bilinear hybrid use of advantage is based on photosensitive and temperature-sensitive sterile line, and male sterile line does not restrict by Rescued virus, and freely, the potential advantages such as output, meter Zhi, resistance of hybrid combination are larger for combo; Male sterile line can one be dual-purpose, does not need maintainer, and production routine simplifies; Sterility mainly controls by nucleic genetic material, has nothing to do with cytoplasm; Photosensitive and temperature-sensitive sterile line is subject to light and temperature condition impact, and crossbreeding exists certain risk.
Summary of the invention
The object of the invention is application High aititude and add Hainan special climate condition reduction round-grained rice type photo-thermo-sensitive genic male sterile line L6S Fertility al-teration origin temp, a kind of propagation method being applicable to Japonica rice low-temperature-sensitive caryon sterile line is provided.
Utilize and come from National Meteorological Bureau's more than 800 meteorological site meteorological data of 50 years, the temperature index of (not occurring for 16 days for three days on end higher than the weather of male sterile line origin temp and daily mean temperature >=18 DEG C) and heading poplar bloassom phase safety (not occurring in 8 days that daily mean temperature is lower than the weather of 20 DEG C for three days on end) can be educated according to different critical temperature of sterility dual purpose genic male sterile line fertility-sensitive period, and 2 safe periods are the time period, adopt slip account form day by day that sensitive periods is started safely that day in day in maximum time as the best fertility-sensitive period time started, filtering out critical temperature of sterility is that to meet probability at Baoshan, Yunnan fertility-sensitive period be 91.67% for the dual purpose genic male sterile line of 22.0 DEG C, heading flowering period meets probability 90.91%, 2 safe periods meet probability 83.34% simultaneously, best August 8 time started sensitive periods.
First find suitable height above sea level at Baoshan City and broadcast slotting period, the individual plant being applicable to male sterile line stable fertility in results proving ground is gathered in the crops.Good for results fertility seed is taken to Hainan and carry out breeding qualification.
For realizing above object, technical scheme steps of the present invention is as follows:
1., in order to the height above sea level that finds the most applicable L6S to breed at Baoshan City and base, arranged breeding test 3 different height above sea levels, 1340m and 1560m respectively plants 0.01hm in 2 bases
2, 1760m base plantation 0.02hm
2, 4 sowing time are divided in 3 different height above sea level bases, each sowing time 4 days, interval.When after heading, first microscopy pollen sterility degree and cover hybridization bag inspection cross-fertile situation, be applicable to the individual plant of male sterile line stable fertility in results proving ground.Is brought to Hainan the seed 10-11 month of results and carry out breeding qualification.
2. good for sterility in step 1 individual plant is broadcast in Hainan slotting by stages, make panicle spike primordium differentiation stage as far as possible cover Hainan cool period, will transplant that rear solid best individual plant seed is mixed to be received by stages, and this period is decided to be carries out record solid best period.The seed part of results takes back Baoshan City, another part stored refrigerated.
3. the seed part of results in step 2 is taken back Baoshan City, plant 1000 group of hill bodies positive season, method is with 1.Observe colony's fertility.
4. if colony's stable fertility, then in step 2, the period of record is best productive phase, the seed that Hainan refrigerates can be planted as the seed of breeding stock sowing time by Second Year, broadcasts the slotting phase, and make it grow under strict isolation condition by best productive phase backstepping arrangement.If colony's fertility instability, then select the good individual plant of sterility to cut the root and stem of certain plants and bring to Hainan, repeat step 2, reselect the period that ripening rate is best.
5. the seed in step 4 is gathered in the crops in time, production of hybrid seeds application can be carried out in target ecotope.
Superiority of the present invention is:
Advantage in the ground that the present invention utilizes Baoshan City uniqueness, ensures that selected male sterile line Fertility al-teration origin temp is 22.5 DEG C; In addition, the special weather conditions in Hainan (Sanya, Hainan 1-2 month, average minimum temperature was at about 20 DEG C) are made full use of; Technical scheme of the present invention is utilized to breed round-grained rice type photo-thermo-sensitive genic male sterile line original seed, its seed purity is all more than 99.9%, and carry out 3-4 breeding cycle continuously and the critical temperature of sterility of male sterile line can be settled out, by the original seed Cord blood produced, the original seed taking out some every year has and is multiplied into the production of hybrid seeds kind, does not need to carry out seed production every year.Overcoming under round-grained rice type photo-thermo-sensitive genic male sterile line cryogenic conditions can the difficult problem of numerous property difference.
Embodiment
Example 1 is bred for L6S in 2012, illustrate utilize Baoshan City High aititude add Hainan special climate carry out round-grained rice type photo-thermo-sensitive genic male sterile line breeding technology.
1. Baoshan City positive season Japonica rice photo-thermo-sensitive genic male sterile line L6S is carried out point height above sea level and sowing in period, object is the special geographical conditions of Baoshan City, ensures that safety cultivates the individual plant of stable fertility.The height above sea level finding the most applicable L6S to breed at Baoshan City and base, arranged breeding test 3 different height above sea levels, 1340m and 1560m respectively plants 0.01hm in 2 bases
2, 1760m base plantation 0.02hm
2, 4 sowing time are divided in 3 different height above sea level bases, each sowing time 4 days, interval.Select the colony of neat and consistent to transplant to Honda, after transplanting, make it grow under strict rich water quality management condition.When after heading, first microscopy pollen sterility degree and cover hybridization bag inspection cross-fertile situation, be applicable to the individual plant of male sterile line stable fertility in results proving ground.October 18 brought to Hainan.
2. good for sterility in step 1 20 individual plants are carried out individual plant in Hainan broadcasts slotting by stages, Different Transplanting Periods is respectively October 19, October 24, October 29, November 4, November 9, November 14, it is February 10 according to solid situation record best productive phase, and by solid grain harvest, seed 0.35kg can be gathered in the crops.Above-mentioned results seed fraction takes back Baoshan City, and another part is for subsequent use in Hainan stored refrigerated.
3. 1000 seeds of results in step 2 are taken back Baoshan City, plant 1000 group of hill bodies positive season, and bagging observes fertility, through qualification, better, the productive phase namely in step 2 is best productive phase to colony's sterility performance.
4. cryopreserved for Hainan in step 2 seed is carried out broadcasting the slotting phase according to best productive phase backstepping to arrange, and make it grow under strict isolation condition, breeding breeder's stock 0.4 mu, every mu of seeding 875 jin, gathers in the crops 350 jin, seed altogether.
5. the breeder's stock of results is carried out the production of hybrid seeds in suitable ecological district.
Claims (1)
1. apply High aititude and add Hainan special climate condition reduction round-grained rice type photo-thermo-sensitive genic male sterile line L6S Fertility al-teration origin temp, a kind of propagation method being applicable to Japonica rice low-temperature-sensitive caryon sterile line is provided.It is characterized in that:
Example 1 is bred for L6S in 2012, illustrate utilize Baoshan City High aititude add Hainan special climate carry out round-grained rice type photo-thermo-sensitive genic male sterile line breeding technology.
(1) Baoshan City positive season Japonica rice photo-thermo-sensitive genic male sterile line L6S is carried out point height above sea level and sowing in period, object is the special geographical conditions of Baoshan City, ensures that safety cultivates the individual plant of stable fertility.The height above sea level finding the most applicable L6S to breed at Baoshan City and base, arranged breeding test 3 different height above sea levels, 1340m and 1560m respectively plants 0.01hm in 2 bases
2, 1760m base plantation 0.02hm
2, 4 sowing time are divided in 3 different height above sea level bases, each sowing time 4 days, interval.Select the colony of neat and consistent to transplant to Honda, after transplanting, make it grow under strict rich water quality management condition.When after heading, first microscopy pollen sterility degree and cover hybridization bag inspection cross-fertile situation, be applicable to the individual plant of male sterile line stable fertility in results proving ground.October 18 brought to Hainan.
(2) good for sterility in step 1 20 individual plants are carried out individual plant in Hainan broadcasts slotting by stages, Different Transplanting Periods is respectively October 19, October 24, October 29, November 4, November 9, November 14, it is February 10 according to solid situation record best productive phase, and by solid grain harvest, seed 0.35kg can be gathered in the crops.Above-mentioned results seed fraction takes back Baoshan City, and another part is for subsequent use in Hainan stored refrigerated.
(3) 1000 seeds of results in step 2 are taken back Baoshan City, plant 1000 group of hill bodies positive season, and bagging observes fertility, through qualification, better, the productive phase namely in step 2 is best productive phase to colony's sterility performance.
(4) cryopreserved for Hainan in step 2 seed is carried out broadcasting the slotting phase according to best productive phase backstepping to arrange, and make it grow under strict isolation condition, breeding breeder's stock 0.4 mu, every mu of seeding 875 jin, gathers in the crops 350 jin, seed altogether.
(5) breeder's stock of results is carried out the production of hybrid seeds in suitable ecological district.
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| CN201410837706.3A CN104542267A (en) | 2014-12-30 | 2014-12-30 | Method for reducing critical temperature of fertility alteration of japonica photo-thermo sensitive genic male sterile line L6S by utilizing conditions of high altitude and special climate of Hainan |
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Cited By (1)
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| CN110100672A (en) * | 2019-06-05 | 2019-08-09 | 湖南隆平种业有限公司 | A kind of method of quick production rice photo-thermo-sensitive sterile line original seed |
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| CN1565154A (en) * | 2003-06-24 | 2005-01-19 | 华中农业大学 | Conservation and breeding method for rice photosensitive and temperature-sensitive sterile line |
| CN101044834A (en) * | 2006-03-27 | 2007-10-03 | 湖南杂交水稻研究中心 | Method for producing hybrid rice seeds of light-temperature sensitive male sterility line by using low-temperature or short-day low temperature |
| CN101999302A (en) * | 2010-10-19 | 2011-04-06 | 湖北省农业科学院粮食作物研究所 | Breeding method of japonica photoperiod/thermo-sensitive genic male sterile lines |
| CN102648690A (en) * | 2011-02-28 | 2012-08-29 | 云南金瑞种业有限公司 | Method for technically propagating dual-purpose genic male sterile rice at high altitudes |
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Patent Citations (4)
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| CN1565154A (en) * | 2003-06-24 | 2005-01-19 | 华中农业大学 | Conservation and breeding method for rice photosensitive and temperature-sensitive sterile line |
| CN101044834A (en) * | 2006-03-27 | 2007-10-03 | 湖南杂交水稻研究中心 | Method for producing hybrid rice seeds of light-temperature sensitive male sterility line by using low-temperature or short-day low temperature |
| CN101999302A (en) * | 2010-10-19 | 2011-04-06 | 湖北省农业科学院粮食作物研究所 | Breeding method of japonica photoperiod/thermo-sensitive genic male sterile lines |
| CN102648690A (en) * | 2011-02-28 | 2012-08-29 | 云南金瑞种业有限公司 | Method for technically propagating dual-purpose genic male sterile rice at high altitudes |
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| CN110100672A (en) * | 2019-06-05 | 2019-08-09 | 湖南隆平种业有限公司 | A kind of method of quick production rice photo-thermo-sensitive sterile line original seed |
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Application publication date: 20150429 |