CN101233231A

CN101233231A - 抗肥胖剂及抗肥胖食品

Info

Publication number: CN101233231A
Application number: CNA2006800273816A
Authority: CN
Inventors: 森田英利; 政冈俊夫; 铃木武人
Original assignee: SCHOOL Corp AZABU VETERINARY MEDICINE EDUCATIONAL INSTITUTION
Current assignee: Nestle Purina PetCare Co
Priority date: 2005-07-26
Filing date: 2006-07-25
Publication date: 2008-07-30
Anticipated expiration: 2026-07-25
Also published as: CN102225078B; CN101370729A; ES2532481T3; US20100216212A1; EP1918373A4; JP2011206057A; EP2604689B1; PL2604689T3; US8637001B2; EP1918373A1; WO2007013438A1; KR20080034474A; CN102225078A; CN101370729B; KR20110095929A; CN101233231B; KR101411144B1; EP1918373B1; US20110311501A1; KR20140043145A

Abstract

本发明提供一种抗肥胖剂，其以属于罗伊氏乳杆菌的微生物为有效成分，该微生物产生由序列表1、3、5所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的脂肪酶。该抗肥胖剂可以在摄取正常饮食的情况下，防止成为肥胖很大原因的脂质被吸收到体内。

Description

抗肥胖剂及抗肥胖食品

技术领域

本发明涉及抗肥胖剂和抗肥胖食品，更详细地说，本发明涉及可以抑制脂质从消化管的摄入、预防肥胖的抗肥胖剂和抗肥胖食品。

背景技术

肥胖是以体脂肪过剩为特征的体重控制体系疾病。现代社会中，由于运动不足和卡路里过剩的饮食导致中性脂肪在体内蓄积并被判定为肥胖的人不断增加，成为很大的问题。

为了预防肥胖，首要的是进行对消耗所摄取的脂肪来说充分的运动，但实际上进行运动较为困难，需要减少脂肪的摄取。

但是，想通过过度的节食来抑制肥胖时，其它必需营养的摄取量不足或平衡被破坏，结果还会对身体造成不良影响。可以说这与摄取被称为减肥食品的营养少但可以带来饱涨感的食品的情况相同。

发明内容

发明要解决的问题

因此，强烈要求开发摄取正常饮食的同时可防止成为肥胖较大原因的脂质(三酰甘油)被吸收到体内的方法。

用于解决问题的方法

本发明人着眼于脂质被吸收到体内的机理，对于肥胖的抑制方法进行了研究，结果注意到，如果肠内的微生物群落(菌群)将脂质摄取分解，则人摄取的脂质减少，自然可以预防肥胖。于是，对于这样的可摄取分解脂质的微生物进行了深入研究，结果发现属于乳酸菌的微生物中具有这种作用的菌，进而完成了本发明。

即，本发明为抗肥胖剂，其以属于罗伊氏乳杆菌的微生物为有效成分，该微生物产生由以下(1)～(3)的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的脂肪酶。这里所说的1个或多个氨基酸缺失、置换或增加的氨基酸序列是指与这些原本的序列等价的序列、依然保持脂肪酶活性的序列。作为这种氨基酸序列可以举出显示80％以上同源性的序列。

(1)M V K L M T I H E L A N N P T L S G Q V R L I E N I V Y G A

M D G E A L H M S I L A P W T Q R F P K Q Y Q T E P R P L I

V F V Q G S S W R T P K M G E E I P Q L V Q F V R A G Y I V

A T V Q H R S S I D S H P F P A F L Q D V K T A I R F L R A

N A Q K Y A I D P Q Q V A I W G T S S G A N A A M L V G L T

G D D P R Y K V D L Y Q D E S D A V D A V V S C F A P M D V

E K T F E Y D A N V P G N K L L Q Y C L L G P D V S K W P E

I E K Q M S P L Y Q V K D G Q N Y P P F L L F H G D A D K V

V P Y E Q M E K M Y M R L K D N G N S V E A Y R V K G A N H

E R D F W S P T I Y N I V Q K F L G D Q F K

(2)L I Y V L K D L C N T I A E V Y G K S I L K G V F I M K H T

L K V D Q V R D G L W L D S D I T Y T Q V P G W L G N T T R

D L K L S V I R H F Q T N D D T R Y P V I F W F A G G G W M

D T D H N V H L P N L V D F A R H G Y I V V G V E Y R D S N

K V Q F P G Q L E D A K A A I R Y M R A N A K R F Q A D P N

R F I V M G E S A G G H M A S M L G V T N G L N Q F D K G A

N L D Y S S D V Q V A V P F Y G V V D P L T A K T G S A S N

D F D F V Y R N L L G A E P E N A P E L D S A A N P L T Y V

N S N S T P F L I F H G T E D V V V P I K D S E K L Y D A L

V E N N V P A E L Y E I E G A S H M D V K F L Q P Q V F K I

V M D F L D K Y L T R S

(3)M E I K S V N L D Q P Y S S L D I Y H S N T D K A L P G L V

I L P G G S Y N Q I M E R D S E R V A L T F A T H A W Q T F

V V R Y P V V E H K N Y E E A K I A V H Q A F E Y I V N H A

A E L D V D A D R L G I I G F S A G G Q I A A A Y S N E K L

T H A R F A A L G Y P V I Q P L I D E R M G V T T E N V A K

L V N P Q T P P T F M W G S A K D E L T P F V D H L Q V Y A

D A L I K N D I P Y E L H E F G T G G H G I A L A N E Y T G

I V N N D R V D N H M G K W F P L F L E W L T E L N L I

另外，本发明为抗肥胖饮食品，其以属于罗伊氏乳杆菌的微生物为有效成分，该微生物产生由上述(1)～(3)的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的脂肪酶。

进而，本发明为由下述(5)～(7)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的亚基所构成的甘油分解酶。这里所说的1个或多个氨基酸缺失、置换或增加的氨基酸序列是指与这些原本的序列等价的序列，依然保持甘油分解活性的序列。作为这种氨基酸序列可以举出显示80％以上同源性的序列。

(5)M K R Q K R F E E L E K R P I H Q D T F V K E W P E E G F V

A M M G P N D P K P S V K V E N G K I V E M D G K K L E D F

D L I D L Y I A K Y G I N I D N V E K V M N M D S T K I A R

M L V D P N V S R D E I I E I T S A L T P A K A E E I I S K

L D F G E M I M A V K K M R P R R K P D N Q C H V T N T V D

N P V Q I A A D A A D A A L R G F P E Q E T T T A V A R Y A

P F N A I S I L I G A Q T G R P G V L T Q C S V E E A T E L

Q L G M R G F T A Y A E T I S V Y G T D R V F T D G D D T P

W S K G F L A S C Y A S R G L K M R F T S G A G S E V L M G

Y P E G K S M L Y L E A R C I L L T K A S G V Q G L Q N G A

V S C I E I P G A V P N G I R E V L G E N L L C M M C D I E

C A S G C D Q A Y S H S D M R R T E R F I G Q F I A G T D Y

I N S G Y S S T P N Y D N T F A G S N T D A M D Y D D M Y V

M E R D L G Q Y Y G I H P V K E E T I I K A R N K A A K A L

Q A V F E D L G L P K I T D E E V E A A T Y A N T H D D M P

K R D M V A D M K A A Q D M M D R G I T A I D I I K A L Y N

H G F K D V A E A I L N L Q K Q K V V G D Y L Q T S S I F D

K D W N V T S A V N D G N D Y Q G P G T G Y R L Y E D K E E

W D R I K D L P F A L D P E H L E L

(6)M A D I D E N L L R K I V K E V L S E T N Q I D T K I D F D

K S N D S T A T A T Q E V Q Q P N S K A V P E K K L D W F Q

P V G E A K P G Y S K D E V V I A V G P A F A T V L D K T E

T G I P H K E V L R Q V I A G I E E E G L K A R V V K V Y R

S S D V A F C A V Q G D H L S G S G I A I G I Q S K G T T V

I H Q K D Q D P L G N L E L F P Q A P V L T P E T Y R A I G

K N A A M Y A K G E S P E P V P A K N D Q L A R I H Y Q A I

S A I M H I R E T H Q V V V G K P E E E I K V T F D

(7)M S E V D D L V A K I M A Q M G N S S S A N S S T G T S T A

S T S K E M T A D D Y P L Y Q K H R D L V K T P K G H N L D

D I N L Q K V V N N Q V D P K E L R I T P E A L K L Q G E I

A A N A G R P A I Q K N L Q R A A E L T R V P D E R V L E M

Y D A L R P F R S T K Q E L L N I A K E L R D K Y D A N V C

A A W F E E A A D Y Y E S R K K L K G D N

进而，本发明为由下述(8)所示的氨基酸序列或在该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的肠道黏附性蛋白质。这里所说的1个或多个氨基酸缺失、置换或增加的氨基酸序列是指与这些原本的序列等价的序列，依然保持使乳酸菌黏附在肠道粘膜细胞附近的活性的序列。作为这种氨基酸序列可以举出显示80％以上同源性的序列。

(8)M F G H D G R I V T K V Y Q W A G T Y Y Y F D P N T Y L R V

D N D Y R Q S Q W G D W Y M F G P D G R I V T G L K E W Y G

S Y Y Y F D P T T Y L K V T N K W I D N K Y F G P A G Q Q A

I S R F E R L D N K Y Y Y F D A N G A V L N I H D Q F K N I

D N H T Y Y F G A D G A C Y T S Q F L N K D G K Q Y Y F D N

D G I M L T D Q E K I I D G K F Y H F N V N G E A I Q V N D

P S E I

发明效果

通过摄取本发明的抗肥胖剂和抗肥胖饮食品，可以进行正常饮食但防止脂质由肠道的吸收。而且，乳酸菌自古就用于酸奶或发酵乳等发酵食品的制造中，安全性极高，因此可以放心地摄取。

附图说明

图1为表示罗伊氏乳杆菌JCM1112T代谢图谱的图。

图2为表示投与罗伊氏乳杆菌JCM1112T的大鼠组随时间的体重增加与比较组和对照组相比较的图。

具体实施方式

作为本发明的抗肥胖剂和抗肥胖食品(以下有时称为“抗肥胖剂等”)的有效成分的微生物，属于乳酸菌的罗伊氏乳杆菌(Lactobacillus reuteri)，是可以产生由上述(1)～(3)的氨基酸序列(分别对应于序列表的1、3、5)或在该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的脂肪酶(以下称为“本发明脂肪酶类”)的乳酸菌(以下称为“本发明乳酸菌”)。

该本发明的脂肪酶类将存在于肠道粘膜细胞附近的脂质(三酰甘油)分解为脂肪酸和甘油，摄入到本发明乳酸菌中。而且，如图1所示，所摄入的脂肪酸作为菌体构成要素被利用，甘油经过各种代谢途径成为二氧化碳、丙酮、乙醇、乳酸盐或罗伊氏素(reuterin)。

本发明乳酸菌除了具有产生上述脂肪酶类的能力，即具有编码上述脂肪酶的核苷酸序列(基因)之外，优选具有以下(4)所示的核苷酸序列(对应于序列表的8)作为编码运载蛋白的基因。另外，本发明还包括与下述(4)所示核苷酸序列的同源性为80％以上、且能在严格条件下与编码具有甘油转运活性的蛋白质的核苷酸序列和下述(4)所示核苷酸序列杂交、且编码具有甘油转运活性的蛋白质的核苷酸序列。

(4)ATGCATGGATTTATTGGCGAATTTTTTGGCACCATGGTTTTAATCCTATTAGGAGCAGGA

TGTTGTGCTGGTAATAGTTTGAATAAAACATATGGGAAACAAAGTGGCTGGTGGTTTATC

TGTATTTCATGGGGCTTAGCAGTTACAATGGGAGTTTATGTTGCAGGATTTCTGGGTTCA

TTAGGGCACTTAAATCCCGCTGTAACAATTCCTTTTGCTATTTTTGGCTTATTCCCATGG

AGTAACGTTATACCTTACTTACTTGGTCAATTTCTTGGTGCGTTTGTTGGTGCAGTATTA

GTAATTATTCAATTCTATCCACAATTTAAAGCAACCCCAAATGAAGAAGAAGGAAATAAT

GTTGGTATTTTTGCTACTCGTCCAGCGATAAATAGTCCAATTTTTAACTTTTTCTCAGAA

GTGATTGCGACCTTTGCATTTATTTTCATCTTATTAAATCTTGGCAACTTTACACAGGGA

TTGAAGCCATTTATCGTAGGAATGGTTATTGCAGTTGTTGGTACATGTCTCGGGACAACT

ACTGGCTTTGCATTAAACCCAGCTCGTGATTGGTCACCACGTTTAGCATATACTATTTTG

CCAATTCCTAATAAGGGTGTTTCAGAATGGTGGTATGCATGGGTTCCAATGTGTGGCCCA

ATTGTTGGGGGCCTTCTTGCTTGTGCTTTACAAACGGCACTAGTTTAG

该基因编码将甘油摄入到本发明乳酸菌的细胞内的运载蛋白，如图1所示，所摄入的甘油通过乳酸菌内的酶代谢。

进而，本发明乳酸菌优选具有由上述式(5)～(7)所示的氨基酸序列(分别对应于序列表的序列号9、11、13)或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的亚基所构成的甘油分解酶。该亚基(5)～(7)所构成的甘油分解酶为在pdu(propanediol utilization)操纵子中发挥作用的甘油脱水酶(glycerol dehydratase)，可以高效地代谢通过本发明脂肪酶的作用所产生的甘油。

另外，可通过基因工程学方法将编码该亚基(5)～(7)的核苷酸序列(9)～(11)(分别对应于序列表的序列号10、12、14)整合到其它乳酸菌等中，由此获得甘油分解性高的乳酸菌。本发明还包括与下述(9)～(11)所示的核苷酸序列的同源性为80％以上、且在严格条件下与编码具有甘油分解活性的蛋白质的核苷酸序列和下述(9)～(11)所示的核苷酸序列杂交、且编码具有甘油分解活性的蛋白质的核苷酸序列。

(9)ATGAAACGTCAAAAACGATTTGAAGAACTAGAAAAACGGCCAATTCATCAAGATACATTT

GTTAAAGAATGGCCAGAAGAAGGTTTCGTTGCAATGATGGGGCCTAATGACCCTAAGCCT

AGTGTAAAAGTTGAAAATGGCAAGATCGTAGAGATGGATGGTAAAAAGCTCGAAGATTTT

GATTTGATTGACTTGTACATTGCTAAGTATGGAATCAATATTGACAACGTTGAAAAAGTT

ATGAATATGGATTCTACCAAGATTGCACGGATGCTTGTTGATCCTAATGTTTCTCGTGAT

GAAATTATTGAAATTACATCAGCTTTGACTCCTGCTAAGGCTGAAGAGATCATCAGTAAG

CTTGATTTTGGTGAAATGATTATGGCTGTCAAGAAGATGCGCCCACGTCGTAAGCCTGAC

AACCAGTGTCACGTTACCAATACTGTTGATAACCCAGTTCAAATTGCTGCTGATGCTGCT

GATGCCGCTCTTCGTGGATTTCCAGAACAAGAAACCACGACAGCTGTGGCACGTTATGCA

CCATTCAATGCTATTTCAATTTTAATTGGTGCACAAACAGGTCGCCCTGGTGTATTGACA

CAATGTTCTGTTGAAGAAGCTACTGAATTGCAATTAGGTATGCGTGGTTTTACCGCATAT

GCTGAAACCATTTCAGTTTACGGTACTGATCGTGTATTTACCGATGGTGATGATACTCCA

TGGTCTAAAGGCTTCTTGGCATCTTGTTATGCATCACGTGGTTTGAAGATGCGATTTACT

TCAGGTGCCGGTTCAGAAGTTTTGATGGGTTATCCAGAAGGTAAGTCAATGCTTTACCTT

GAAGCGCGTTGTATTTTACTTACTAAGGCTTCAGGTGTTCAAGGACTTCAAAATGGTGCC

GTAAGTTGTATTGAAATTCCTGGTGCTGTTCCTAATGGTATTCGTGAAGTTCTCGGTGAA

AACTTGTTATGTATGATGTGTGACATCGAATGTGCTTCTGGTTGTGACCAAGCATACTCA

CACTCCGATATGCGGCGGACTGAACGGTTTATTGGTCAATTTATTGCCGGTACTGATTAT

ATTAACTCTGGTTACTCATCAACTCCTAACTACGATAATACCTTCGCTGGTTCAAACACT

GATGCTATGGACTACGATGATATGTATGTTATGGAACGTGACTTGGGTCAATATTATGGT

ATTCACCCTGTTAAGGAAGAAACCATTATTAAGGCACGTAATAAGGCCGCTAAAGCCCTT

CAAGCAGTATTTGAAGATCTTGGATTACCAAAGATTACTGATGAAGAGGTCGAAGCAGCA

ACGTATGCTAACACCCATGATGACATGCCAAAGCGGGATATGGTTGCAGATATGAAGGCT

GCTCAAGATATGATGGATCGTGGAATTACTGCTATTGATATTATCAAGGCATTGTACAAC

CACGGATTTAAAGATGTCGCTGAAGCAATTTTGAACCTTCAAAAACAAAAAGTTGTTGGT

GATTACCTTCAAACATCTTCTATTTTTGATAAAGATTGGAACGTCACTTCTGCTGTTAAC

GACGGAAATGATTATCAAGGACCAGGTACTGGATACCGTCTATATGAAGACAAGGAAGAA

TGGGATCGGATTAAAGACTTACCATTCGCCCTTGATCCAGAACATTTGGAACTGTAG

(10)ATGGCTGATATTGATGAAAACTTATTACGTAAAATCGTTAAAGAAGTTTTAAGCGAAACT

AATCAAATCGATACTAAGATTGACTTTGATAAAAGTAATGATAGTACTGCAACAGCAACT

CAAGAGGTGCAACAACCAAATAGTAAAGCTGTTCCAGAAAAGAAACTTGACTGGTTCCAA

CCAGTTGGAGAAGCAAAACCTGGATATTCTAAGGATGAAGTTGTAATTGCAGTCGGTCCT

GCATTCGCAACTGTTCTTGATAAGACAGAAACTGGTATTCCTCATAAAGAAGTGCTTCGT

CAAGTTATTGCTGGTATTGAAGAAGAAGGGCTTAAGGCGCGGGTAGTTAAAGTTTACCGG

AGTTCAGATGTAGCATTCTGTGCTGTCCAAGGTGATCACCTTTCTGGTTCAGGAATTGCT

ATTGGTATCCAATCAAAAGGGACGACAGTTATTCACCAAAAGGATCAAGACCCTCTTGGT

AACCTTGAGTTATTCCCACAAGCGCCAGTACTTACTCCCGAAACTTATCGTGCAATTGGT

AAGAATGCCGCTATGTATGCTAAGGGTGAATCTCCAGAACCAGTTCCAGCTAAAAACGAT

CAACTTGCTCGTATTCACTATCAAGCTATTTCAGCAATTATGCATATTCGTGAAACTCAC

CAAGTTGTTGTTGGTAAGCCTGAAGAAGAAATTAAGGTTACGTTTGATTAA

(11)ATGAGTGAAGTTGATGATTTAGTAGCAAAGATCATGGCTCAGATGGGAAACAGTTCATCT

GCTAATAGCTCTACAGGTACTTCAACTGCAAGTACTAGTAAGGAAATGACAGCAGATGAT

TACCCACTTTATCAAAAGCACCGTGATTTAGTAAAAACACCAAAAGGACACAATCTTGAT

GACATCAATTTACAAAAAGTAGTAAATAATCAAGTTGATCCTAAGGAATTACGGATTACA

CCAGAAGCATTGAAACTTCAAGGTGAAATTGCAGCTAATGCTGGCCGTCCAGCTATTCAA

AAGAATCTTCAACGAGCTGCAGAATTAACACGAGTACCTGACGAACGGGTTCTTGAAATG

TATGATGCATTGCGTCCTTTCCGTTCAACTAAGCAAGAATTATTGAACATTGCAAAGGAA

TTACGGGACAAGTATGACGCTAATGTTTGCGCAGCATGGTTTGAAGAAGCTGCTGATTAT

TATGAAAGTCGTAAGAAGCTAAAGGGCGATAACTAA

本发明乳酸菌优选进一步还保有下述(8)的氨基酸序列(对应于序列表的序列号15)或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的肠道黏附性蛋白质。

(8)M F G H D G R I V T K V Y Q W A G T Y Y Y F D P N T Y L R V

D N D Y R Q S Q W G D W Y M F G P D G R I V T G L K E W Y G

S Y Y Y F D P T T Y L K V T N K W I D N K Y F G P A G Q Q A

I S R F E R L D N K Y Y Y F D A N G A V L N I H D Q F K N I

D N H T Y Y F G A D G A C Y T S Q F L N K D G K Q Y Y F D N

D G I M L T D Q E K I I D G K F Y H F N V N G E A I Q V N D

P S E I

该肠道黏附性蛋白质具有使乳酸菌黏附在肠道粘膜细胞附近的作用，具有该蛋白质的本发明乳酸菌在肠道粘膜附近存在一定期间，可以稳定地摄入脂质。另外，可通过公知的方法将编码该蛋白质的下述(12)所示的基因(对应于序列表的序列号16)整合到其它乳酸菌中，由此获得在肠道内滞留时间长的乳酸菌。另外，本发明还包括与下述(12)所示的核苷酸序列的同源性为80％以上、且在严格条件下与编码具有使乳酸菌黏附在肠道粘膜细胞附近的活性的蛋白质的核苷酸序列和下述(12)所示的核苷酸序列杂交、且编码具有使乳酸菌黏附在肠道粘膜细胞附近的活性的蛋白质的核苷酸序列。

(12)ATGTTCGGTCACGATGGCCGCATTGTTACTAAAGTTTACCAATGGGCTGGCACGTATTAC

TACTTTGATCCGAATACTTATTTGCGAGTAGATAATGATTACCGTCAATCTCAGTGGGGC

GATTGGTATATGTTTGGCCCAGATGGTCGTATCGTTACAGGGTTAAAGGAATGGTACGGT

AGTTATTATTACTTTGATCCGACGACTTACTTAAAAGTAACTAATAAGTGGATAGATAAT

AAGTACTTTGGTCCAGCTGGTCAGCAAGCTATTTCACGCTTTGAGAGACTTGATAATAAG

TATTACTATTTCGATGCTAATGGGGCAGTTCTTAATATCCATGATCAATTTAAGAATATT

GATAACCACACTTATTACTTTGGAGCTGATGGTGCTTGTTATACCAGTCAATTCTTAAAT

AAGGATGGTAAACAGTATTATTTCGATAATGATGGAATTATGCTCACTGATCAAGAGAAG

ATCATTGACGGTAAATTCTATCATTTCAATGTTAATGGTGAAGCAATCCAAGTAAATGAT

CCTTCTGAAATTTGA

另外，本发明乳酸菌优选具有由下述式(16)～(20)任一个所示的氨基酸序列(分别对应于序列表的序列号17、19、21、23、25)或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的甘油分解酶。该甘油分解酶为醇脱氢酶(alcohol dehydrogenase，图1中的ADH(8))，可以高效地代谢由本发明脂肪酶的作用所产生的甘油。这里所说的1个或多个氨基酸缺失、置换或增加的氨基酸序列是指与这些原本的序列等价的序列，依然保持甘油分解活性的序列。作为这种氨基酸序列，可以举出显示80％以上同源性的序列。

(16)M K A A V I N D P V D G F V T V K D V Q L R D L K P G E A L

V D M E Y C G L C H T D L H V A A G D F G K K P G R I I G H

E G V G R V S K V A P G V T S L K V G D R V S I A W F F K G

C G H C E Y C L T G R E T L C R N V L N A G Y T A D G A M A

E Q C I V P A D Y A V K V P E G L D P V E A T S L T C A G V

T M Y K A L K V A D I K P G Q W V S I V G A G G L G N L G I

Q L A H N V F G A H V I A V D G N P D K L E A A K K N G A E

I L I N R H D G D V D K Q I Q E K V G G V H A A V V T A V S

A S A F D Q A V D S L R P D G K L V A V A L P Q G D M K L N

I A K T V L D G I I V A G S L V G T R Q D L A E C F Q F G A

E G K V H P I V K T R K L S E I N D M I Q E L K D N K V V G

R N V V D F V H N D N D

(17)M E K R E N A I P K T M K A W A V T T P G P I D G K E S P I

E F T E K P V P T P K R G E V L V K V I T C G V C H T D L H

V T E G D L P V H H E H V T P G H E I V G K V V G F G P E T

Q R F K F G E R I G I P W F R H A C G V C K F C R S G H E N

L C P H S L Y T G W D H D G G Y A E Y V T V P E G F A Y R L

P E K F D S L E A A P L L C A G I I G Y R A F E R A N V P A

G G R L G L Y G F G G S A H I T A Q I A L A Q G I E V H V F

T R G E D A K K F A L E L G C A S V Q G S Y D P A P V P L D

S S I I F A P V G D M V L P A L A S L V P G G T L A L A G I

H M T D I P T M N Y Q K E I F H E K T L T S V E S N T R R D

G E E F L T L A D R L N I H P E V H E Y P L A K A D E A L R

Y V K H G D I K G A C V L R V S E D

(18)M Q I K A A L A T K P N A D L E I Q T V E L D E P K E N E V

L I K I A S T G F C H T D I V G R S G A T T P L P V V L G H

E G A G V V Q K V G A N V T D V K P G D H V V L S F S Y C G

H C Y N C T H N H Q G L C E N F N Q L N F E G K T Y D G T H

R L H L D D G T P V S V F F G Q S S F A T Y V T A N V H N I

V K V D Q D V D L N L L G P L G C G M Q T G A G T V L N Y I

K P A P E D A I A V F G A G A V G L A A I M A A K I A G V K

H I I A I N R N G N H L D L A K E L G A T E T I N N T A E D

P V K A I K E I V P R G V T Y A I D T T G N T G V I K S A I

D S L A T A G E C V L L G V G G D I T L D L M N D I L S E S

K K I S G V V E G D S N P Q E F I P Q L V K Y Y K Q S K F P

L D K L V K Y Y D F A D I N Q V I A D S T N G K V I K P I I

K I D P E L A K L P L T N D G S N V Q K M V A E A G L A D Q

I T I D S A G T S N I A E G S P A D S R T K A I L D K Y H I

K D D G M I A R Q L Q D R D Y Y D A D Y I I A M D Q M N V R

D A K D M A P A G L E N K V H G I F E A T P G K E N C Y I V

D P W I T H

(19)M K K A I F E K A G Q M K I V D V D R P T I E K P D D V I I

K V V R T C V C G S D L W N F R G I N P V E K D S E N S G H

E A I G I V E E V G E D I T T V K P G D F V I A P F T H G C

G H C A A C R A G F D G S C Q S H N D N F S S G V Q A Q Y V

R F Q H G Q W A L V K V P G K P S D Y S E G M L K S L L T L

A D V M A T G Y H A A R V A N V S D G D T V V V M G D G A V

G L C A I I A A K M R G A K K I I S T S R H A D R Q A L A K

E F G A T D N V A E R S D E A V Q K I M E L T N G A G A D A

V L E C V G T E Q S T D T A M K V G R P G T I V G R V G L P

H T P K M D M T V L F Y N N T I V G G G P A S V T T Y D K D

V L L K A V L D G D I N P G K V F T K S F D L D Q I Q E A Y

E A M D K R E A I K S Y I I M D G F E R D

(20)M G R L D N K V A I I T G G S K G I G A A V A K K F I E E G

A K V V L T A R K M D E G Q K V A D Q L G D N A I F I Q Q D

V A R K G D W D R V I R Q T V Q V F G K L N I V V N N A G I

A E Y A D V E K T D A E I W D K T I A V N L T G T M W G T K

L G I E A M K N N G E K N S I I N M S S I E G L I G D P D L

F A Y N A S K G G V R L L T K S A A L D C A R K G Y D I R V

N T I H P G Y I S T P L V D N L V K D D P K A E G H L E S L

H P L G R L G K P E E I A N L A L Y L A S D E S S F S T G S

E F V A D G G Y T A Q

另外，还可以通过基因工程学的方法将编码该甘油分解酶(16)～(20)的下述核苷酸序列(32)～(36)(分别对应于序列表的序列号18、20、22、24、26)整合到其它乳酸菌等，由此获得甘油分解性高的乳酸菌。另外，本发明还包括与下述(32)～(36)任一个所示的核苷酸序列的同源性为80％以上、且在严格条件下与编码具有甘油分解活性的蛋白质的核苷酸序列和下述(32)～(36)任一个所示的核苷酸序列杂交、且编码具有甘油分解活性的蛋白质的核苷酸序列。

(32)ATGAAAGCTGCTGTTATTAATGATCCAGTAGACGGTTTTGTTACTGTTAAAGATGTTCAA

CTTCGGGATTTGAAGCCCGGTGAAGCTTTAGTTGACATGGAATATTGTGGTCTTTGTCAC

ACTGATCTACACGTTGCTGCTGGGGACTTTGGTAAGAAGCCCGGTCGTATTATCGGTCAC

GAAGGGGTTGGTCGTGTATCTAAGGTTGCCCCTGGCGTTACTTCCTTGAAAGTTGGCGAC

CGTGTATCAATTGCATGGTTCTTCAAGGGCTGTGGACACTGTGAATATTGTTTAACTGGT

CGTGAAACTCTTTGTCGGAACGTTCTTAATGCGGGTTACACTGCTGACGGTGCAATGGCT

GAACAATGTATCGTACCAGCTGACTACGCTGTTAAGGTTCCAGAAGGTCTTGATCCTGTT

GAAGCTACTTCATTAACTTGTGCTGGTGTTACGATGTACAAGGCATTAAAGGTTGCTGAC

ATCAAGCCAGGTCAATGGGTATCAATCGTTGGTGCTGGTGGTTTAGGTAACTTGGGTATT

CAACTTGCTCACAACGTATTTGGTGCTCATGTTATCGCTGTTGATGGTAATCCTGATAAG

CTTGAAGCCGCTAAGAAGAATGGTGCTGAAATTTTAATTAACCGTCATGACGGTGATGTT

GATAAGCAAATTCAAGAAAAGGTTGGCGGTGTTCACGCTGCTGTAGTAACAGCTGTTTCT

GCCTCTGCATTCGACCAAGCAGTTGATTCACTTCGCCCAGATGGTAAGCTTGTTGCCGTT

GCGCTTCCACAAGGTGACATGAAGCTTAACATTGCTAAGACTGTTCTTGATGGTATCATT

GTTGCTGGTTCATTAGTTGGTACCCGTCAAGACTTAGCTGAATGTTTCCAATTTGGTGCA

GAAGGTAAGGTTCACCCAATTGTTAAGACTCGTAAGTTAAGCGAAATTAATGATATGATC

CAAGAACTTAAGGATAACAAGGTTGTTGGTCGGAATGTTGTTGATTTTGTTCACAACGAT

AACGACTAA

(33)ATGGAAAAACGCGAAAATGCTATTCCGAAAACAATGAAGGCTTGGGCAGTCACAACTCCT

GGGCCGATTGATGGTAAGGAATCACCAATCGAATTTACCGAAAAGCCTGTGCCGACTCCT

AAACGGGGAGAAGTCCTTGTTAAGGTAATAACGTGTGGAGTATGTCATACGGACTTGCAC

GTGACTGAAGGAGACTTGCCGGTTCACCACGAACACGTTACTCCTGGTCATGAAATTGTT

GGTAAAGTTGTCGGCTTTGGACCAGAGACACAACGATTTAAGTTTGGTGAGCGAATTGGG

ATTCCATGGTTTCGGCATGCTTGTGGTGTATGCAAGTTTTGCCGATCAGGTCATGAGAAT

CTCTGTCCTCATTCACTTTATACCGGTTGGGATCATGATGGCGGTTATGCAGAATATGTC

ACAGTTCCAGAAGGATTTGCATATCGGCTTCCAGAAAAGTTTGATTCCCTAGAGGCAGCT

CCGTTATTATGTGCAGGGATTATTGGTTATCGGGCCTTTGAACGTGCCAATGTTCCGGCT

GGCGGTCGCCTAGGATTATATGGCTTCGGTGGTTCAGCTCATATTACAGCTCAAATTGCA

CTTGCTCAGGGAATTGAAGTGCATGTCTTTACGCGTGGTGAGGATGCCAAGAAATTCGCC

CTAGAATTAGGTTGTGCTTCTGTTCAGGGCTCCTATGACCCAGCACCAGTTCCTTTGGAT

TCATCAATCATTTTTGCGCCGGTTGGTGATATGGTCTTGCCGGCTTTAGCTAGTTTAGTT

CCAGGGGGGACATTAGCATTAGCCGGTATTCATATGACTGATATTCCAACAATGAATTAC

CAAAAAGAAATATTCCACGAAAAGACATTAACGAGTGTTGAGAGTAATACTCGTCGTGAT

GGGGAAGAATTCTTAACATTAGCTGATCGTCTTAATATCCATCCTGAAGTCCACGAATAT

CCCCTAGCAAAGGCTGACGAAGCATTACGCTATGTTAAGCACGGTGATATTAAGGGAGCT

TGTGTATTACGTGTTAGTGAGGACTAA

(34)ATGCAAATTAAAGCTGCTCTTGCAACCAAACCTAACGCTGATTTAGAGATTCAAACCGTC

GAATTGGATGAACCAAAAGAAAATGAAGTATTAATAAAAATTGCTTCAACAGGTTTTTGT

CATACAGATATTGTTGGTCGAAGCGGTGCCACTACCCCTCTCCCCGTTGTCCTCGGGCAT

GAAGGTGCGGGCGTCGTCCAAAAAGTAGGAGCTAACGTTACGGACGTTAAACCCGGCGAC

CATGTTGTTCTATCATTTAGCTACTGTGGCCATTGCTATAACTGTACTCATAATCATCAA

GGCTTATGCGAAAACTTCAATCAGCTAAACTTTGAAGGAAAAACCTATGATGGTACTCAC

CGCCTGCACTTAGATGATGGCACGCCAGTCAGTGTCTTTTTTGGTCAGTCTTCCTTTGCG

ACCTATGTAACAGCCAATGTCCATAATATTGTTAAAGTTGATCAAGATGTTGATCTTAAC

TTATTAGGGCCACTCGGTTGTGGAATGCAAACAGGTGCTGGAACCGTTCTAAATTATATT

AAACCTGCTCCTGAAGATGCAATTGCCGTTTTCGGTGCTGGTGCTGTTGGCTTAGCCGCA

ATTATGGCTGCTAAAATTGCTGGAGTTAAACATATTATTGCGATTAATCGTAACGGTAAC

CACCTTGACCTGGCGAAGGAATTGGGCGCTACTGAAACGATTAATAATACGGCTGAAGAT

CCCGTCAAAGCAATTAAAGAAATCGTTCCGCGTGGTGTAACTTATGCAATCGATACTACC

GGAAACACCGGTGTAATTAAATCAGCAATTGATAGTCTTGCCACCGCTGGAGAATGTGTC

CTCTTAGGAGTTGGCGGCGATATTACCTTAGACTTAATGAATGATATCTTATCAGAATCT

AAGAAAATCTCTGGGGTTGTCGAAGGAGATAGCAATCCCCAAGAGTTTATTCCTCAACTA

GTTAAGTACTACAAGCAAAGCAAGTTCCCCCTTGATAAGCTTGTTAAGTACTACGATTTT

GCTGATATTAACCAAGTTATCGCTGACTCAACAAACGGAAAGGTTATTAAGCCAATCATC

AAAATTGATCCTGAATTAGCTAAATAATTGCCGCTCACCAATGACGGAAGCAATGTTCAA

AAAATGGTTGCAGAAGCTGGCCTTGCTGATCAAATTACTATTGATTCAGCCGGAACAAGT

AACATTGCAGAAGGTTCACCTGCTGATAGTCGAACAAAAGCCATTCTCGATAAATATCAC

ATTAAAGACGACGGAATGATTGCCCGTCAATTGCAGGACAGGGATTATTATGATGCCGAT

TATATTATCGCAATGGATCAGATGAATGTCCGGGACGCAAAAGATATGGCACCAGCTGGG

TTAGAAAATAAGGTTCATGGAATCTTTGAAGCTACCCCAGGAAAAGAAAATTGCTATATC

GTTGACCCCTGGATCACTCACTGA

(35)ATGAAAAAAGCTATTTTTGAAAAGGCGGGTCAAATGAAGATTGTTGATGTTGACCGTCCA

ACAATTGAAAAGCCTGATGACGTAATTATTAAGGTAGTGCGGACCTGTGTTTGTGGTTCT

GACCTATGGAACTTCCGAGGAATTAATCCGGTTGAAAAAGATTCTGAAAACTCTGGCCAT

GAAGCAATTGGAATTGTTGAAGAAGTTGGTGAAGATATCACTACTGTCAAACCTGGGGAC

TTTGTGATTGCTCCATTTACTCATGGATGTGGGCACTGTGCTGCTTGTCGCGCGGGCTTC

GATGGTTCTTGCCAAAGTCACAACGATAACTTTAGCTCTGGTGTGCAAGCTCAATACGTT

CGGTTCCAACACGGTCAATGGGCGCTTGTTAAAGTTCCGGGCAAGCCAAGTGACTACAGT

GAAGGAATGCTTAAGTCCCTCTTAACCCTTGCTGATGTTATGGCTACTGGTTACCACGCT

GCACGAGTTGCTAACGTTAGTGATGGTGATACAGTTGTTGTAATGGGTGACGGTGCTGTT

GGCCTTTGTGCGATTATTGCTGCTAAGATGCGGGGCGCTAAGAAGATCATTTCTACTAGT

CGCCATGCTGACCGTCAAGCCCTTGCTAAGGAATTTGGTGCTACTGACAATGTTGCTGAA

CGTAGTGACGAAGCGGTTCAAAAGATCATGGAACTCACTAACGGTGCCGGTGCTGATGCT

GTCCTTGAATGCGTTGGTACTGAACAATCAACTGATACTGCCATGAAAGTTGGCCGTCCA

GGTACCATCGTTGGTCGGGTTGGCTTACCTCATACCCCAAAGATGGACATGACGGTGCTA

TTCTACAACAACACTATTGTCGGCGGTGGTCCAGCATCAGTAACCACTTACGACAAGGAC

GTATTGTTGAAGGCTGTTCTTGATGGTGACATTAACCCTGGTAAGGTCTTTACTAAGAGC

TTCGACCTTGACCAAATTCAAGAAGCTTATGAAGCAATGGATAAGCGTGAAGCAATCAAG

TCTTACATTATTATGGATGGCTTTGAACGCGATTAA

(36)ATGGGTCGTTTAGATAATAAAGTTGCAATTATTACTGGTGGTTCTAAAGGAATTGGAGCT

GCTGTCGCAAAAAAGTTTATCGAAGAAGGCGCAAAGGTTGTTTTAACCGCTCGGAAGATG

GATGAGGGACAAAAAGTCGCTGACCAACTAGGTGACAATGCGATCTTTATCCAACAAGAC

GTTGCTCGGAAAGGAGACTGGGACCGGGTAATCCGCCAAACTGTCCAAGTCTTTGGGAAG

CTCAATATTGTGGTTAACAATGCGGGAATTGCCGAATACGCCGATGTTGAGAAGACGGAC

GCTGAAATTTGGGATAAAACAATTGCCGTTAACCTTACCGGTACGATGTGGGGAACTAAG

CTCGGTATTGAAGCAATGAAGAACAACGGGGAAAAGAATTCAATCATCAATATGTCATCC

ATTGAAGGACTAATTGGTGATCCTGATCTCTTTGCATACAATGCTTCTAAGGGTGGTGTC

CGCCTCTTAACTAAGTCCGCTGCGCTTGATTGTGCCCGGAAAGGCTATGACATCCGTGTA

AATACAATTCATCCTGGTTATATCTCAACTCCACTAGTTGATAATTTGGTCAAGGATGAT

CCAAAAGCAGAAGGACACCTAGAAAGCCTTCATCCCCTTGGCCGTCTTGGAAAGCCAGAA

GAGATTGCTAACCTCGCTTTATACCTTGCTTCAGATGAATCAAGCTTTAGTACTGGTTCG

GAATTTGTCGCTGATGGTGGCTATACGGCTCAATAA

本发明乳酸菌优选具有由下述式(21)或(22)所示的氨基酸序列(分别对应于序列表的序列号27、29)或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的甘油分解酶。该甘油分解酶为醇脱氢酶(alcohol dehydrogenase，图1中的ADH(8))，可以高效地代谢由本发明的脂肪酶作用所产生的甘油。这里所说的1个或多个氨基酸缺失、置换或增加的氨基酸序列是指与这些原本的序列等价的序列，依然保持甘油分解活性的序列。作为这种氨基酸序列，可以举出显示80％以上同源性的序列。

(21)M T N V P T V K L N N G V E M P T L G F E V F Q V P D L S Q

A E Q A V T D A L E V G Y R L I D T A A A Y Q N E E A V G K

A I K N S S V N R E D V F V T S K L W V S D F N Y K R A K A

G I D A S L Q K L G L D Y M D L Y L L H Q P Y G D T M G A W

R A L Q E A Q K E G K I R A I G V S N F Y A D Q L K D L E L

T M P V K P A V N Q I E V N P W Y Q Q D Q E V K F A Q S E D

I R V E A W A P F A E G K H D I F T N E I I A E I A A K Y G

K S N G Q V I L R W L L Q R G I T V I P K S V H K N R M E E

N I D V F D F E L S N D D M K K I A S L N K K E S Q F F D H

R D P V T I E Q I F G S S L K M V Q D D E K

(22)M I L D E T I T L N S G V K I P K F A L G T W M I D D D Q A

A E A V R N A I K M G Y R H I D T A Q A Y D N E R G V G E G

V R T A G I D R D K I F V T S K I A A E H K D Y D V T K K S

I D E T L E K M G L D Y I D M M L I H S P Q P W K E V N Q S

D N R Y L E G N L A A W R A M E D A V N E G K I R T I G V S

N F K K A D L E N I I K N S D T V P A V D Q V L A H I G H T

P F N L L S F T H E H D I A V E A Y S P V A H G A A L D N P

V I E K M A K K Y N V S V P Q L C I R Y D W Q I G M I V L P

K T T N P E H M K E N T E I D F E I S E A D M D L L R R V K

P L D Y G D F D I Y P V Y G G K M

另外，还可以通过基因工程学的方法将编码该甘油分解酶(21)或(22)的下述核苷酸序列(37)或(38)(分别对应于序列表的序列号28、30)整合到其它乳酸菌等，由此获得甘油分解性高的乳酸菌。另外，本发明还包括与下述(37)或(38)所示的核苷酸序列的同源性为80％以上、且在严格条件下与编码具有甘油分解活性的蛋白质的核苷酸序列和下述(37)或(38)所示的核苷酸序列杂交、且编码具有甘油分解活性的蛋白质的核苷酸序列。

(37)ATGACAAATGTACCAACAGTAAAATTAAATAACGGAGTAGAAATGCCAACCCTTGGATTT

GAAGTATTCCAAGTTCCAGACTTAAGCCAAGCTGAACAAGCAGTTACCGATGCTCTTGAA

GTCGGCTATCGTTTAATCGATACTGCTGCTGCTTACCAAAATGAAGAAGCAGTTGGAAAG

GCAATTAAGAATAGTAGTGTAAACCGTGAAGATGTCTTTGTAACTTCTAAGTTATGGGTG

TCTGATTTTAACTATAAGCGGGCTAAAGCAGGGATTGACGCTTCACTGCAAAAACTTGGC

CTTGATTACATGGATCTTTACCTTCTCCATCAACCATATGGCGATACAATGGGGGCTTGG

CGAGCATTACAAGAAGCACAGAAAGAAGGTAAGATTCGCGCAATCGGTGTATCGAACTTC

TACGCTGATCAACTAAAGGATCTTGAATTAACAATGCCTGTTAAGCCAGCGGTCAACCAA

ATTGAAGTTAACCCTTGGTACCAGCAAGATCAAGAGGTTAAGTTTGCGCAAAGTGAAGAT

ATTCGTGTTGAAGCATGGGCACCATTTGCGGAAGGTAAGCATGATATTTTTACCAACGAA

ATAATTGCGGAAATTGCTGCCAAGTATGGCAAGAGCAATGGTCAAGTAATTCTTCGCTGG

CTTTTACAACGGGGTATTACTGTCATTCCAAAGTCAGTCCACAAGAACCGGATGGAAGAA

AATATCGATGTCTTTGATTTTGAACTTTCCAATGATGATATGAAAAAGATAGCTAGTCTT

AACAAGAAGGAAAGCCAATTCTTTGACCACCGTGATCCGGTTACGATTGAACAAATCTTT

GGCTCCAGCTTAAAGATGGTTCAAGATGACGAAAAATAA

(38)ATGATTTTAGATGAGACAATTACTCTTAATAGTGGTGTGAAAATTCCAAAGTTTGCATTA

GGAACCTGGATGATTGATGATGACCAAGCAGCCGAAGCAGTTCGGAATGCGATTAAGATG

GGATATCGGCACATCGATACAGCTCAGGCTTATGATAATGAGCGGGGAGTCGGTGAAGGT

GTACGAACAGCCGGTATTGATCGGGATAAAATCTTTGTTACTTCAAAGATCGCTGCTGAA

CACAAAGATTATGATGTAACTAAAAAGTCGATTGACGAGACTCTTGAAAAGATGGGTCTT

GATTATATCGACATGATGCTTATTCATAGTCCTCAACCATGGAAAGAAGTAAATCAATCT

GATAATCGTTACCTTGAAGGAAATCTCGCTGCTTGGCGAGCCATGGAAGATGCCGTTAAC

GAAGGTAAGATTCGAACAATTGGCGTTTCTAATTTCAAAAAAGCCGATCTTGAAAATATT

ATTAAGAATAGCGATACCGTTCCCGCTGTTGATCAAGTTTTAGCTCATATTGGTCATACT

CCATTCAATCTTTTATCATTTACTCATGAACATGACATTGCGGTTGAAGCATATTCACCA

GTTGCTCACGGCGCTGCTTTAGACAACCCCGTAATTGAAAAGATGGCTAAAAAGTACAAC

GTTTCAGTCCCACAATTGTGCATTCGGTATGATTGGCAAATAGGAATGATCGTCTTACCA

AAGACTACTAATCCAGAACACATGAAGGAAAACACTGAAATTGATTTTGAAATTTCTGAA

GCTGATATGGACCTATTGCGGCGAGTAAAGCCATTAGACTATGGCGATTTTGATATCTAC

CCTGTTTACGGTGGAAAAATGTAA

另外，本发明乳酸菌优选具有由下述式(23)～(25)任一个所示的氨基酸序列(分别对应于序列表的序列号31、33、35)或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的醛脱氢酶(aldehyde dehydrogenase)。该醛脱氢酶为图1中所示的aldehyde dehydrogenase(9)，可以高效地代谢由本发明脂肪酶的作用而产生的甘油。这里所说的1个或多个氨基酸缺失、置换或增加的氨基酸序列是指与这些原本的序列等价的序列，依然保持醛脱氢酶活性的序列。作为这种氨基酸序列可以举出显示80％以上同源性的序列。

(23)M P A N N K K Q V E K K E L T A E E K K Q N A Q K L V D D L

M T K S Q A A F E K L R Y Y S Q E Q V D K I C Q A M A L A A

E E H H M D L A V D A A N E T G R G V A E D K A I K N I Y A

S E Y I W N N I R H D K T V G I I E D N D E D Q T I K I A D

P L G V I A G I V P V T N P T S T T I F K S I I S A K T R N

T I I F S F H R Q A M K S S I K T A K I L Q E A A E K A G A

P K N M I Q W L P E S T R E N T T A L L Q H P N T A T I L A

T G G P S L V K A A Y S S G N P A L G V G P G N G P A Y I E

K T A N I E R S V Y D I V L S K T F D N G M I C A T E N S V

V V D E E I Y D K V K E E F Q K W N C Y F L K P N E I D K F

T D G F I D P D R H Q V R G P I A G R S A N A I A D M C G I

K V P D N T K V I I A E Y E G V G D K Y P L S A E K L S P V

L T M Y K A T S H E N A F D I C A Q L L H Y G G E G H T A A

I H T L D D D L A T K Y G L E M R A S R I I V N S P S G I G

G I G N I Y N N M T P S L T L G T G S Y G S N S I S H N V T

D W D L L N I K T I A K R R E N R Q W V K I P P K V Y F Q R

N S L K E L Q D I P N I N R A F I V T G P G M S K R G Y V Q

R V I D Q L R Q R Q N N T A F L V F D D V E E D P S T N T V

E K G V A M M N D F K P D T I I A L G G G S P M D A A K A M

W M F Y E H P E T S W Y G V M Q K Y L D I R K R A Y Q I K K

P T K S Q L I G I P T T S G T G S E V T P F A V I T D S K T

H V K Y P L A D Y A L T P N I A I V D S Q F V E T V P A K T

T A W T G L D V L C H A T E S Y V S V M A T D Y T R G W S L

Q T I K G V M E N L P K S V Q G D K L A R R K M H D F S T M

A G M A F G Q A F L G I N H S L A H K M G G A F G L P H G L

L I A I A M P Q V I R F N A K R P Q K L A L W P H Y E T Y H

A T K D Y A D I A R F I G L K G N T D E E L A E A Y A K K V

I E L A H E C G V K L S L K D N G V T R E E F D K A V D D L

A R L A Y E D Q C T T T N P V E P L V S Q L K E L L E R C Y

D G T G V E E K

(24)M A Y Q S I N P F T N Q V E K T F E N T T D E E L E Q T L T

T A H Q L Y L D W R K Y N D L E E R K R Q I L K L G Q I L R

E R R V E Y A T V M S K E M G K L I S E A E G E V D L C A S

F C D Y Y A A H A D E F L Q P K I I A T T S G R A K V L K Q

S L G I L V A V E P W N F P F Y Q I A R V F I P N F I A G N

P M I L K D A S N C P A S A Q A F N D A V K E A G A P A G S

L T N L F L S Y D Q V N K A I A D K R V A G V C L T G S E R

G G A T V A K E A G A N L K K S T L E L G G N D A F I I L D

D A D W D L V E K V A P A A R L Y N A G Q V C T S S K R F I

V L E K D Y D R F L K M M K D A F S K V K M G D P L D P L T

T L A P L S S K K A K E K L Q Q Q V A T A V E N G A K V Y Y

G N K P V D M E G Q F F M P T I L T D I T P D N P I F D T E

M F G P V A S V Y K V S S E E E A I E L A N N S S Y G L G N

T I F S N D S E H A E R V A A K I E T G M S W I N A G W A S

L P E L P F G G V K N S G Y G R E L S S Y G I D E F T N K H

L I Y E A R Q

(25)M Q I N D I E S A V R K I L A E E L D N A S S S S A N V A A

T T D N G H R G I F T N V N D A I A A A K A A Q E I Y R D K

P I A V R Q Q V I D A I K E G F R P Y I E K M A K D I K E E

T G M G T V E A K I A K L N N A L Y N T P G P E I L E P V V

E N G D G G M V M Y E R L P Y G V I G A V G P S T N P S E T

V I A N A I M M L A G G N T L Y F G A H P G A K N V T R W T

I E K M N D F I A D A T G L H N L V V S I E T P T I E S V Q

Q M M K H P D I A M L A V T G G P A V V H Q A M T S G K K A

V G A G P G N P P A M V D A T A D I D L A A H N I I T S A S

F D N D I L C T A E K E V V A E S S I K D E L I R K M Q D E

G A F V V N R E Q A D K L A D M C I Q E N G A P D R K F V G

K D A T Y I L D Q A N I P Y T G H P V E I I C E L P K E H P

L V M T E M L M P I L P V V S C P T F D D V L K T A V E V E

K G N H H T A T I H S N N L K H I N N A A H R M Q C S I F V

V N G P S Y V G T G V A D N G A H S G A S A L T I A T P T G

E G T C T A R T F T R R V R L N S P Q G F S V R N W Y

另外，还可以通过基因工程学的方法将编码该醛脱氢酶(23)～(25)的下述核苷酸序列(39)～(41)(分别对应于序列表的序列号32、34、36)整合到其它乳酸菌等，由此获得甘油分解性高的乳酸菌。另外，本发明还包括与下述(39)～(41)任一个所示的核苷酸序列的同源性为80％以上、且在严格条件下与编码具有醛脱氢酶活性的蛋白质的核苷酸序列和下述(39)～(41)任一个所示的核苷酸序列杂交、且编码具有醛脱氢酶活性的蛋白质的核苷酸序列。

(39)ATGCCTGCTAACAACAAGAAACAAGTTGAAAAGAAAGAATTAACTGCTGAAGAAAAAAAG

CAAAACGCCCAAAAGCTAGTTGACGATTTAATGACTAAGAGTCAAGCTGCTTTTGAAAAG

TTACGTTACTATTCACAAGAACAAGTTGACAAGATTTGTCAGGCAATGGCTCTCGCTGCC

GAAGAACACCACATGGACTTAGCTGTTGATGCTGCTAACGAAACTGGTCGTGGGGTTGCT

GAAGATAAGGCTATCAAGAACATCTACGCAAGTGAATACATTTGGAACAACATCCGTCAC

GATAAGACTGTTGGTATTATCGAAGACAATGATGAAGACCAAACTATCAAAATTGCTGAT

CCACTTGGTGTCATTGCCGGAATTGTTCCAGTTACTAACCCTACTTCAACAACGATCTTC

AAATCAATCATTAGTGCTAAGACACGGAATACAATCATCTTTTCTTTCCACCGTCAAGCA

ATGAAGTCATCTATCAAGACTGCAAAGATTCTCCAAGAAGCTGCTGAAAAAGCCGGTGCG

CCAAAGAACATGATTCAATGGCTCCCTGAAAGTACCCGCGAAAACACTACCGCATTACTC

CAACACCCTAATACTGCTACTATTTTAGCAACCGGTGGTCCTTCATTAGTTAAGGCTGCC

TACAGTTCTGGTAACCCTGCTCTTGGTGTTGGTCCTGGTAACGGTCCTGCTTACATCGAA

AAAACTGCCAACATCGAACGTTCTGTTTACGACATCGTTCTTTCTAAGACATTCGATAAC

GGTATGATTTGTGCCACTGAAAACTCAGTTGTTGTTGATGAAGAAATCTACGACAAGGTT

AAAGAAGAATTCCAAAAGTGGAACTGTTACTTCTTGAAGCCAAACGAAATTGATAAATTT

ACTGATGGCTTTATTGACCCAGATCGTCATCAAGTTCGTGGTCCAATCGCTGGTCGTTCA

GCTAATGCTATTGCTGACATGTGTGGTATTAAAGTACCTGACAACACTAAGGTTATCATT

GCTGAATACGAAGGTGTTGGTGACAAGTACCCACTTTCAGCTGAAAAGCTTTCACCAGTA

TTAACAATGTACAAGGCAACCTCTCACGAAAATGCCTTTGATATCTGTGCTCAATTATTA

CACTACGGTGGTGAAGGTCACACTGCTGCTATTCACACCCTTGATGATGATTTAGCTACT

AAGTACGGTCTTGAAATGCGTGCTTCACGGATCATTGTTAACTCCCCATCTGGTATTGGT

GGTATTGGTAACATCTACAACAACATGACTCCATCCCTTACTTTAGGTACTGGTTCATAC

GGTAGTAACTCAATTTCTCACAACGTTACTGATTGGGACCTCTTAAACATCAAAACAATT

GCAAAGCGGCGTGAAAACCGTCAATGGGTTAAGATTCCCCCAAAAGTATACTTTCAACGC

AACTCACTAAAAGAATTGCAAGATATTCCAAACATTAACCGGGCATTCATCGTTACTGGT

CCTGGAATGAGCAAGCGTGGTTACGTTCAACGTGTTATCGATCAATTGCGTCAACGCCAA

AACAACACTGCTTTCTTAGTATTTGATGACGTTGAAGAAGATCCATCAACAAACACTGTT

GAAAAAGGTGTTGCCATGATGAATGACTTCAAACCTGATACAATTATTGCTCTTGGTGGT

GGTTCACCAATGGATGCTGCTAAGGCTATGTGGATGTTCTATGAGCACCCAGAAACTTCA

TGGTATGGGGTTATGCAAAAGTACCTTGATATTCGGAAGCGTGCTTACCAAATCAAGAAG

CCTACTAAGTCTCAACTTATTGGTATCCCTACTACATCAGGTACTGGTTCAGAAGTTACT

CCATTTGCGGTTATTACCGATTCAAAAACTCATGTTAAGTACCCACTTGCTGACTACGCC

TTAACACCAAACATTGCAATCGTTGACTCACAATTCGTTGAAACTGTTCCAGCAAAAACT

ACTGCTTGGACTGGACTAGATGTTTTATGTCACGCTACTGAATCATATGTTTCTGTTATG

GCAACTGACTACACTCGTGGTTGGTCACTACAAACCATCAAGGGTGTTATGGAAAACCTT

CCTAAGTCAGTTCAAGGTGATAAGTTAGCTCGTCGTAAGATGCACGACTTCTCAACAATG

GCCGGGATGGCATTTGGTCAAGCCTTCTTAGGAATTAACCACTCCCTTGCCCACAAGATG

GGTGGAGCATTCGGTCTTCCTCACGGTTTGCTTATCGCTATTGCAATGCCACAAGTAATT

CGCTTTAACGCAAAACGTCCACAAAAGCTTGCTCTCTGGCCTCACTATGAGACTTACCAT

GCAACTAAGGACTACGCTGACATTGCACGGTTCATTGGTTTGAAAGGCAACACTGATGAA

GAATTAGCTGAAGCATATGCTAAGAAAGTTATCGAACTTGCTCACGAATGTGGTGTTAAG

CTTAGTCTTAAGGACAATGGTGTTACACGTGAAGAATTTGATAAGGCGGTTGACGATCTT

GCTCGCTTAGCTTACGAAGATCAATGTACTACTACTAACCCAGTTGAACCACTTGTTAGC

CAACTCAAGGAATTACTTGAACGTTGCTACGATGGTACTGGCGTTGAAGAAAAATAA

(40)ATGGCATATCAAAGTATCAATCCATTTACGAACCAAGTAGAAAAAACGTTTGAAAATACA

ACTGATGAAGAATTAGAACAAACATTAACTACGGCGCATCAATTATATTTAGATTGGCGG

AAGTATAATGACCTTGAAGAACGGAAACGGCAAATTTTAAAGTTAGGTCAAATATTACGT

GAACGGCGTGTTGAATATGCGACAGTTATGAGTAAGGAAATGGGAAAATTAATTAGCGAA

GCAGAAGGCGAGGTTGACCTTTGTGCTTCTTTCTGTGATTATTATGCAGCCCATGCAGAT

GAATTTCTGCAACCAAAAATTATTGCGACAACGAGTGGACGCGCCAAAGTTTTGAAGCAA

TCATTAGGAATTTTAGTTGCAGTTGAACCTTGGAATTTCCCATTCTATCAAATTGCCCGG

GTATTTATTCCCAACTTTATTGCAGGAAACCCCATGATCTTGAAGGATGCGTCGAATTGT

CCAGCATCCGCCCAAGCATTTAACGATGCCGTTAAGGAAGCTGGTGCGCCAGCCGGCAGT

TTAACTAATTTATTCCTTTCATATGACCAAGTAAATAAGGCAATTGCTGATAAGCGGGTA

GCCGGCGTTTGTCTTACTGGTTCTGAACGTGGTGGTGCAACCGTTGCTAAAGAGGCTGGT

GCTAATTTGAAGAAGAGCACTTTGGAACTTGGTGGTAATGATGCCTTTATTATCTTAGAC

GATGCAGATTGGGATCTTGTCGAAAAAGTTGCCCCGGCAGCCCGTCTGTATAATGCTGGA

CAAGTATGTACATCATCAAAACGTTTTATTGTCCTTGAAAAGGATTATGATCGTTTCTTA

AAGATGATGAAAGATGCGTTCTCGAAAGTTAAAATGGGTGATCCCCTTGATCCATTAACA

ACTCTGGCACCATTATCATCTAAGAAAGCAAAAGAAAAGCTCCAACAGCAAGTCGCAACA

GCAGTAGAAAATGGGGCCAAAGTTTACTATGGTAATAAGCCGGTTGACATGGAAGGTCAA

TTCTTTATGCCAACGATCTTAACTGATATCACTCCAGATAACCCAATATTTGATACGGAA

ATGTTTGGGCCAGTGGCTTCGGTTTATAAGGTTAGTTCCGAAGAGGAAGCAATCGAACTG

GCTAATAATTCAAGCTATGGGTTAGGAAACACTATCTTTAGCAATGATTCCGAACATGCG

GAACGAGTAGCAGCGAAGATCGAAACTGGAATGAGTTGGATTAATGCCGGCTGGGCTTCA

TTACCAGAATTACCATTTGGTGGTGTTAAGAATTCAGGTTACGGTCGTGAACTCAGCAGT

TACGGAATTGATGAATTTACTAACAAACATCTAATTTACGAAGCACGACAATAA

(41)ATGCAGATTAATGATATTGAAAGTGCTGTACGCAAAATTCTTGCCGAAGAACTAGATAAT

GCCAGCTCTTCAAGTGCAAACGTTGCAGCTACTACTGATAATGGTCATCGCGGAATTTTC

ACTAATGTCAATGATGCAATTGCTGCTGCAAAAGCTGCTCAAGAAATATATCGGGATAAG

CCAATTGCTGTTCGCCAACAAGTGATTGATGCCATTAAGGAAGGATTCCGCCCATATATT

GAAAAAATGGCTAAAGATATCAAAGAAGAAACAGGAATGGGAACAGTAGAGGCCAAAATT

GCTAAGTTAAACAATGCCTTGTACAACACTCCTGGTCCCGAGATTCTTGAACCAGTTGTA

GAAAACGGTGACGGTGGGATGGTTATGTATGAACGGTTACCATATGGTGTTATTGGTGCG

GTTGGCCCAAGTACAAACCCTTCAGAAACTGTAATTGCTAATGCGATCATGATGCTTGCC

GGTGGTAATACTCTTTACTTTGGTGCTCACCCTGGCGCAAAGAATGTTACTCGCTGGACA

ATTGAAAAGATGAACGATTTTATTGCAGATGCAACAGGCCTTCATAATTTAGTTGTAAGT

ATTGAAACACCAACAATTGAATCAGTTCAACAAATGATGAAGCACCCCGACATTGCAATG

TTAGCAGTAACTGGTGGCCCAGCTGTTGTTCACCAAGCAATGACCAGTGGTAAGAAAGCG

GTTGGTGCTGGTCCTGGTAATCCTCCTGCAATGGTTGATGCTACTGCTGATATTGATTTA

GCTGCTCATAATATCATTACATCTGCTTCATTTGATAATGATATTTTATGTACTGCTGAA

AAGGAAGTAGTTGCAGAAAGTAGCATTAAAGATGAATTAATTCGTAAGATGCAAGATGAA

GGTGCCTTTGTAGTTAACCGTGAACAAGCCGATAAATTAGCTGATATGTGTATCCAAGAA

AATGGTGCTCCTGATCGTAAATTTGTTGGTAAGGATGCAACTTATATCTTAGACCAAGCT

AATATTCCTTACACAGGCCACCCAGTTGAAATTATTTGTGAACTTCCTAAGGAACATCCA

TTAGTAATGACTGAAATGTTAATGCCAATTTTACCAGTTGTTTCTTGTCCAACATTTGAT

GATGTTTTGAAGACTGCTGTTGAAGTTGAAAAAGGTAACCATCACACAGCTACTATTCAT

TCCAATAACCTTAAGCATATTAATAATGCTGCTCACCGGATGCAATGTTCAATCTTTGTT

GTTAATGGCCCATCCTATGTTGGTACAGGTGTTGCAGATAATGGAGCTCACTCAGGTGCT

TCAGCATTAACAATTGCTACGCCAACTGGTGAAGGAACATGTACTGCACGAACATTTACT

CGTCGGGTTCGTTTGAACTCACCACAAGGATTCTCAGTACGTAACTGGTATTAA

本发明乳酸菌优选具有由下述式(26)所示的氨基酸序列(对应于序列表的序列号37)或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的甘油酸激酶(glycerate kinase)。该甘油酸激酶为图1中所示的glyceratekinase(10)，可以高效地代谢由本发明脂肪酶的作用而产生的甘油。这里所说的1个或多个氨基酸缺失、置换或增加的氨基酸序列是指与这些原本的序列等价的序列，依然保持甘油酸激酶活性的序列。作为这种氨基酸序列可以举出显示80％以上同源性的序列。

(26)M K F V I A P D S F K G G L T A K E A A N V M A E G I K R V

F P N A E Y A L V P M A D G G E G T V Q S L V D A T N G Q K

M I A K V H N P L N K L V N A E Y G I L G D G E T A V I E M

A A A S G L Q F V N K E T A N P L I T T T Y G T G E L I K D

A L D H N I K K I I I G I G G S A T V D G G A G M A Q A L G

A R L L D A D N H E I G L G G G E L A S L E Q V D F G G L D

P R L K N V D I Q I A S D V T N P L T G K N G A A P V F G P

Q K G A D E E M V N I L D K N L H H Y A R K I V A A G G P D

V E Q T A G A G A A G G L G A G L I A F T G A T M K R G V E

L V I E A T Q L Q K K A V G A D Y V F T G E G G I D F Q T K

F G K T P Y G V A K A T K E V A P T A P V I V L A G N I G K

G V N D L Y S S T A I D A I F A T P E G A K P L K T A L A D

A P I D I A Q T A E N V A R L I K V S H V S N

另外，还可以通过基因工程学的方法将编码该甘油酸激酶(26)的下述核苷酸序列(42)(对应于序列表的序列号38)整合到其它乳酸菌等，由此获得甘油分解性高的乳酸菌。另外，本发明还包括与下述(42)所示的核苷酸序列的同源性为80％以上、且在严格条件下与编码具有甘油酸激酶活性的蛋白质的核苷酸序列和下述(42)所示的核苷酸序列杂交、且编码具有甘油酸激酶活性的蛋白质的核苷酸序列。

(42)ATGAAATTTGTAATTGCTCCAGATTCATTTAAAGGCGGATTAACAGCAAAAGAAGCAGCA

AATGTGATGGCAGAAGGAATCAAAAGAGTGTTTCCGAATGCCGAGTATGCTTTAGTTCCA

ATGGCTGATGGAGGAGAGGGGACTGTTGAATCCTTAGTTGATGCGACTAACGGTCAAAAA

ATGATTGCTAAAGTCCACAACCCATTAAATAAATTAGTTAATGCTGAGTACGGAATATTA

GGTGATGGGGAAACGGCAGTGATTGAGATGGCGGCGGCAAGTGGCCTTCAATTTGTTAAT

AAGGAGACTGCGAACCCGCTTATTACAACTACATATGGTACCGGCGAGTTAATTAAGGAT

GCTCTTGACCATAACATTAAAAAAATAATTATTGGAATTGGTGGAAGTGCAACCGTTGAT

GGCGGAGCGGGGATGGCCCAAGCACTTGGAGCACGTTTATTGGATGCTGATAATCATGAA

ATTGGTTTAGGCGGTGGTGAGTTAGCAAGTTTAGAGCAAGTAGATTTTGGAGGATTAGAT

CCTCGCTTAAAAAATGTAGATATTCAGATTGCATCAGACGTAACCAACCCATTAACAGGA

AAAAATGGGGCAGCCCCAGTATTTGGCCCGCAAAAAGGAGCTGATGAAGAAATGGTGAAC

ATCTTGGACAAAAATCTTCATCATTATGCCCGAAAAATAGTTGCAGCTGGTGGGCCAGAC

GTTGAACAAACGGCAGGTGCAGGGGCAGCCGGTGGTTTAGGAGCCGGGTTGATAGCATTT

ACCGGTGCGACAATGAAGCGAGGAGTAGAATTAGTGATTGAAGCAACTCAACTACAAAAA

AAGGCAGTTGGCGCTGATTATGTTTTTACTGGTGAAGGAGGAATTGATTTCCAGACTAAA

TTTGGTAAAACGCCATATGGAGTCGCTAAGGCAACTAAAGAGGTGGCTCCAACTGCTCCG

GTAATTGTGTTGGCTGGAAATATTGGTAAAGGCGTAAATGATCTATATTCATCCACGGCC

ATTGATGCAATTTTTGCAACTCCTGAAGGGGCTAAACCATTAAAAACAGCATTAGCAGAT

GCACCTATTGATATTGCTCAAACAGCGGAAAACGTTGCACGTTTAATTAAAGTGAGTCAT

GTTAGTAATTAA

本发明乳酸菌优选具有由下述式(27)所示的氨基酸序列(对应于序列表的序列号39)或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的甘油激酶(glycerolkinase)。该甘油激酶为图1中所示的GK(5)，可以高效地代谢由本发明脂肪酶的作用而产生的甘油。这里所说的1个或多个氨基酸缺失、置换或增加的氨基酸序列是指与这些原本的序列等价的序列，依然保持甘油激酶活性的序列。作为这种氨基酸序列可以举出显示80％以上同源性的序列。

(27)L S E Q Q Y I M A I D Q G T T S S R A I I F D H D G N K V A

I S Q Q E F P Q Y F P Q P G W V E H D P L E I W D S V Q S V

I S N V M I K S Q I K P Y K I A A I G I T N Q R E T T V I W

D R H T G K P I Y N A I V W Q S K Q T S D I A E Q L I K D G

Y K D M I H Q K T G L V I D S Y F A A T K I K W I L D H V P

G A R E K A A K G D L M F G T I D T W L L W N L S G R R V H

A T D V T N A S R T M L F N I H T L D W D Q D I L D L L D I

P Q S L L P V V K P S S A I Y G Y T G D Y H F Y G V Q I P I

A G I A G D Q Q A A L F G Q A A Y D K G S I K N T Y G T G A

F I V M N T G L K P T L S D N G L L T T I A Y G L D G Q T H

Y A L E G S I F V A G S A V Q W L R D G L K M F D K A S E S

E Q M A V D A K T T G G V Y V V P A F T G L G A P Y W D Q E

V R G A M F G L T R G T E R G H I I R A T L E A I A Y Q T K

D V V D T M V K D T Q L P L T A L T V N G G A S R N N F M M

Q F Q A D I L Q T P I K R A A M E E T T A L G A A F L A G L

A V D F W E D Q D E L R K L S R I G D Q F D P Q M D P Q K A

A D L Y R G W Q R A I A A A Q F Y G K D

另外，还可以通过基因工程学的方法将编码该甘油激酶(27)的下述核苷酸序列(43)(对应于序列表的序列号40)整合到其它乳酸菌等，由此获得甘油分解性高的乳酸菌。另外，本发明还包括与下述(43)所示的核苷酸序列的同源性为80％以上、且在严格条件下与编码具有甘油激酶活性的蛋白质的核苷酸序列和下述(43)所示的核苷酸序列杂交、且编码具有甘油激酶活性的蛋白质的核苷酸序列。

(43)TTGAGTGAACAACAATATATCATGGCGATTGACCAGGGAACGACGAGCTCACGGGCGATT

ATCTTTGACCATGACGGAAATAAGGTTGCGATCAGTCAGCAGGAATTTCCCCAATACTTC

CCGCAGCCGGGGTGGGTTGAACATGATCCTCTAGAGATTTGGGATAGCGTTCAATCAGTG

ATTTCAAATGTAATGATTAAGTCCCAGATCAAGCCCTATAAGATTGCGGCAATTGGGATT

ACTAACCAACGGGAGACGACGGTTATTTGGGATCGCCATACCGGTAAGCCGATTTATAAC

GCAATTGTCTGGCAATCGAAGCAAACGAGCGACATCGCCGAACAATTGATTAAAGATGGT

TATAAGGATATGATCCACCAGAAGACTGGCTTGGTGATTGATTCGTATTTCGCGGCCACT

AAGATCAAGTGGATCCTTGACCATGTTCCTGGTGCCCGGGAAAAAGCAGCAAAGGGAGAC

TTGATGTTTGGGACTATCGATACTTGGTTACTATGGAAHTATCGGGACGGCGGGTCCAC

GCAACGGATGTGACCAATGCCAGCCGGACGATGCTTTTTAATATCCATACCCTCGACTGG

GATCAAGATATCCTTGACCTGCTTGATATTCCCCAGTCGCTTTTGCCAGTAGTAAAGCCA

AGTTCAGCCATTTACGGTTATACTGGCGACTACCACTTCTATGGGGTGCAGATTCCAATT

GCCGGGATTGCAGGTGACCAACAAGCAGCCCTCTTTGGTCAAGCAGCCTATGATAAAGGT

TCAATCAAGAACACCTATGGGACTGGAGCCTTCATCGTCATGAATACGGGACTAAAACCC

ACGCTTTCGGATAACGGCTTGTTGACGACGATTGCGTATGGCCTGGACGGGCAAACTCAT

TACGCGCTTGAAGGAAGTATCTTTGTGGCCGGTTCTGCCGTTCAATGGTTGCGGGATGGT

CTCAAGATGTTTGATAAGGCAAGCGAGTCCGAACAAATGGCTGTCGATGCCAAGACAACT

GGCGGCGTTTATGTCGTCCCCGCCTTTACAGGATTAGGCGCACCGTACTGGGATCAAGAA

GTGCGGGGCGCAATGTTTGGCCTTACCCGTGGAACTGAACGGGGACATATCATCCGTGCA

ACTTTGGAAGCCATTGCCTACCAGACCAAAGATGTTGTCGATACGATGGTCAAGGACACC

CAATTACCACTAACAGCACTAACGGTTAACGGGGGCGCTTCACGGAACAACTTCATGATG

CAGTTCCAGGCCGATATCTTACAAACGCCAATCAAGCGGGCAGCAATGGAAGAGACAACC

GCGCTGGGAGCAGCCTTTCTCGCTGGATTGGCCGTTGATTTCTGGGAAGACCAGGATGAG

TTACGGAAGCTATCACGGATTGGCGACCAGTTTGATCCACAAATGGATCCGCAAAAGGCA

GCTGACTTGTATCGGGGATGGCAACGGGCCATTGCAGCTGCGCAGTTTTATGGCAAAGAT

TAA

本发明乳酸菌优选具有由下述式(28)所示的氨基酸序列(对应于序列表的序列号41)或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的甘油-3-磷酸脱氢酶(glycerol-3-phosphate dehydrogenase)。该甘油-3-磷酸脱氢酶为图1中所示的GPD(6)，可以高效地代谢由本发明脂肪酶的作用而产生的甘油。这里所说的1个或多个氨基酸缺失、置换或增加的氨基酸序列是指与这些原本的序列等价的序列，依然保持甘油-3-磷酸脱氢酶活性的序列。作为这种氨基酸序列可以举出显示80％以上同源性的序列。

(28)M A E K I A V L G A G S W G S V L A N M L T E N G H D V T L

W S R N E E Q V K Q L N T E H T N P R Y M K D F V Y S T N L

T A T T D M K K A V K G A S V V L I V I P T K G L R E V A K

Q L N A I L T E L H Q K P L V I H A T K G L E Q N T Y K R P

S E M L S E D I S P E N R Q A I V V L S G P S H A E D V A I

K D M T A V T A A C E D L A S A K K A Q K L F S N S Y F R V

Y T N D D V I G A E F G A A L K N I I A I G A G A I Q G L G

Y H D N A R A A L I T R G L A E I R R L G V A F G A N P M T

F I G L S G V G D L V V T A T S K N S R N W R A G Y Q L G Q

G K K L Q D V I D N M G M V I E G V Y T T K A A Y E L S R K

R Q V Q M P I T E A L Y R V L Y E G E D I K T A I S Q L M D

R D L T S E N E

另外，还可以通过基因工程学的方法将编码该甘油-3-磷酸脱氢酶(28)的下述核苷酸序列(44)(对应于序列表的序列号42)整合到其它乳酸菌等，由此获得甘油分解性高的乳酸菌。另外，本发明还包括与下述(44)所示的核苷酸序列的同源性为80％以上、且在严格条件下与编码具有甘油-3-磷酸脱氢酶活性的蛋白质的核苷酸序列和下述(44)所示的核苷酸序列杂交、且编码具有甘油-3-磷酸脱氢酶活性的蛋白质的核苷酸序列。

(44)ATGGCAGAAAAAATTGCTGTTTTAGGTGCTGGTTCGTGGGGCAGTGTTTTAGCAAACATG

CTTACAGAAAATGGCCACGATGTAACATTATGGTCTCGTAATGAGGAACAAGTTAAGCAA

TTAAATACTGAACATACAAATCCTCGCTATATGAAAGATTTTGTTTATTCTACTAACTTA

ACAGCAACAACGGACATGAAAAAAGCTGTTAAGGGTGCCAGTGTGGTCCTGATTGTAATT

CCAACAAAGGGTCTTCGTGAAGTTGCTAAGCAATTAAATGCAATTTTGACTGAATTACAT

CAAAAACCGCTAGTTATTCACGCAACGAAAGGCTTAGAACAAAATACTTATAAGCGGCCA

TCGGAAATGCTTAGCGAAGATATTTCTCCTGAAAACCGTCAGGCAATTGTTGTTTTATCA

GGTCCGAGTCATGCTGAAGATGTGGCGATTAAAGATATGACAGCTGTAACCGCAGCTTGT

GAGGACCTGGCCAGTGCTAAAAAGGCGCAGAAGTTATTTAGTAATTCTTATTTCCGTGTG

TACACTAATGACGATGTAATTGGTGCCGAATTTGGCGCAGCCTTAAAGAACATTATTGCA

ATTGGTGCTGGAGCTATTCAGGGACTTGGTTATCATGATAATGCTCGGGCAGCGTTAATT

ACTCGTGGACTTGCAGAAATTCGCCGATTGGGAGTTGCTTTTGGTGCCAACCCGATGACT

TTTATTGGTCTTTCTGGGGTTGGTGACCTTGTTGTTACTGCTACCAGTAAAAATTCTCGA

AATTGGCGTGCTGGCTATCAATTGGGGCAAGGAAAAAAGCTTCAAGATGTAATTGATAAT

ATGGGAATGGTTATCGAAGGTGTCTATACTACCAAAGCCGCTTATGAATTAAGTCGTAAA

CGACAAGTACAGATGCCAATTACCGAAGCTCTTTACCGTGTTTTGTATGAAGGCGAAGAT

ATTAAAACTGCAATTTCTCAATTAATGGACCGAGATCTTACTTCAGAAAACGAATAA

本发明乳酸菌优选具有由下述式(29)～(31)任一个所示的氨基酸序列(对应于序列表的序列号43、45、47)或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的磷酸丙糖异构酶(triosephosphate isomerase)。该磷酸丙糖异构酶为图1中所示的triosephosphate isomerase(15)，可以高效地代谢由本发明脂肪酶的作用而产生的甘油。这里所说的1个或多个氨基酸缺失、置换或增加的氨基酸序列是指与这些原本的序列等价的序列，依然保持磷酸丙糖异构酶活性的序列。作为这种氨基酸序列可以举出显示80％以上同源性的序列。

(29)M R K P F I A A N W K M H K N V Q E S V E F V D A I K G K L

P D P Q E V E V G I A A Q A F A L P S M V Q A A D D S G L K

I I A Q N A A A E Y S G A F T G E I S L R G L A D A G V S Y

V M L G H I E R R H L F H E D N E L V N R K V L A A L Q M G

V T P I I C T D E T M V Q K E V N G E I H Y V F Q Q L M S V

L R G V S L D Q I K N V V V S Y E P S W A V G Y G Q H A N P

V L A E E G C R Q I R R T I A D N Y T Y E I A D K I R I L Y

G G S V N P D N I G M I M N K P D V D G V L I G R A S L D V

D N F L R M V N Y L K N D Q E K

(30)M R K P F I I A N W K M N K N V H E S V A F V K A I K E K L

P A D K E I G I A A Q A V S L Y N M K K V A S S S N L Q I I

A Q N A S A E L E G P Y T G E I S M R S L A D A G V T Y V M

L G H L E R R R L F N E S N D S I N Q K V L A A L N A G I I

P I I C T D E E M V Q T E V N G Q I H Y V F R Q L K S V L K

G V P A N K L S Q I V I S Y E P S W A V G S T H Q A N P D I

A E E G C Q A I R Q S L V E M Y G N E I G E Q V R I L Y G G

S V N P E N I G Q I M S K P N V D G A L I G R A S L E I E S

F L Q M I N Y I E L A S K Q K L Q V I

(31)M R V P I I A G N W K M H K D V Q E A V S F I E K V K N Q L

P P A D Q L E T A I A A P T L C L V P M V K A A E E S P L K

I M A E N C Y Y K N E G A Y T G E T S P Y A L Y Q A G I H H

V I L G H S E R R T Y F N E T D E L I N K K V K A A L V N G

L C P I V C C D D T M R R R V A G K K V H W V V S R I L A D

L H G L T N D E I C H V T V A Y E P S W A I G T G E S A D P

E Q A A E G C Y L I R Q T I S D M Y G D E V A N N V R I L Y

G G S V T T S N I N A L M A K N D I D G V L V G A A S L N P

E T F L Q L V H H

另外，还可以通过基因工程学的方法将编码该磷酸丙糖异构酶(29)～(31)的下述核苷酸序列(45)～(47)(对应于序列表的序列号44、46、48)整合到其它乳酸菌等，由此获得甘油分解性高的乳酸菌。另外，本发明还包括与下述(45)～(47)任一个所示的核苷酸序列的同源性为80％以上、且在严格条件下与编码具有磷酸丙糖异构酶活性的蛋白质的核苷酸序列和下述(45)～(47)任一个所示的核苷酸序列杂交、且编码具有磷酸丙糖异构酶活性的蛋白质的核苷酸序列。

(45)ATGCGCAAACCCTTTATTGCTGCTAATTGGAAGATGCATAAGAATGTCCAAGAATCGGTT

GAATTTGTGGATGCAATTAAAGGAAAGCTACCAGATCCGCAAGAAGTTGAAGTCGGAATT

GCAGCCCAAGCTTTTGCATTACCCAGTATGGTTCAAGCCGCTGATGATTCAGGATTAAAG

ATAATCGCGCAAAACGCGGCGGCTGAATATTCGGGAGCTTTCACTGGTGAAATTAGCTTA

CGAGGTTTAGCTGACGCCGGTGTTTCATATGTAATGTTAGGACATATTGAACGGCGCCAT

TTATTCCACGAGGATAATGAGTTGGTTAATCGGAAAGTGTTGGCAGCCCTTCAAATGGGA

GTTACCCCGATAATTTGTACGGATGAAACGATGGTCCAGAAAGAAGTTAATGGTGAAATT

CACTACGTTTTCCAGCAATTGATGAGCGTATTGAGGGGCGTTTCTCTTGATCAAATTAAA

AATGTAGTTGTTTCCTATGAACCAAGTTGGGCAGTTGGATATGGTCAGCATGCTAATCCA

GTTCTTGCTGAAGAAGGATGCCGTCAAATTCGGCGAACGATTGCTGATAACTACACTTAT

GAGATTGCTGATAAGATCAGGATTCTTTATGGGGGCAGTGTCAATCCAGATAATATCGGA

ATGATTATGAACAAGCCAGATGTAGATGGGGTATTAATCGGTCGGGCAAGTTTAGATGTT

GATAATTTTTTGCGAATGGTCAATTATTTAAAAAATGATCAAGAAAAATAA

(46)ATGCGCAAACCGTTTATTATTGCGAACTGGAAAATGAATAAAAACGTTCATGAATCTGTT

GCGTTTGTTAAAGCAATTAAAGAAAAGCTCCCGGCAGATAAAGAAATTGGGATCGCCGCG

CAAGCAGTTTCGCTATATAACATGAAAAAAGTGGCGAGCTCTTCCAACTTACAAATTATT

GCTCAAAATGCATCTGCTGAGTTAGAGGGACCATATACTGGAGAAATTAGCATGCGAAGT

TTAGCAGATGCGGGCGTGACATACGTGATGCTAGGCCATTTAGAGCGCCGACGCCTTTTT

AACGAGAGTAATGATTCAATTAATCAAAAAGTTTTAGCAGCCCTCAATGCTGGTATTATT

CCAATCATTTGTACGGATGAAGAGATGGTCCAAACAGAAGTTAACGGACAAATTCATTAT

GTATTTCGCCAACTAAAAAGCGTCCTTAAAGGGGTACCAGCTAATAAACTATCACAGATT

GTTATTTCGTATGAACCAAGTTGGGCCGTTGGGAGCACGCATCAAGCAAATCCAGACATT

GCGGAAGAGGGATGTCAGGCAATTCGTCAAAGCCTGGTTGAAATGTATGGTAATGAGATT

GGCGAGCAAGTCCGAATACTCTATGGTGGCAGCGTTAATCCCGAGAACATTGGTCAAATT

ATGAGTAAACCAAATGTTGATGGGGCGCTAATCGGTCGCGCAAGTCTCGAGATTGAAAGT

TTCTTACAAATGATTAATTATATCGAATTAGCGAGCAAGCAGAAGTTACAGGTAATTTAG

(47)ATGAGAGTACCGATTATTGCTGGTAATTGGAAAATGCATAAGGATGTACAAGAAGCTGTC

TCTTTTATCGAAAAAGTAAAAAATCAGCTTCCGCCTGCCGACCAACTTGAAACAGCAATT

GCTGCTCCTACTCTTTGTTTAGTACCAATGGTTAAAGCAGCTGAAGAATCCCCGTTAAAA

ATAATGGCAGAAAACTGCTACTATAAGAATGAGGGAGCTTATACTGGTGAAACAAGTCCA

TATGCTTTATACCAAGCAGGAATCCATCATGTGATTTTAGGCCATTCTGAACGCCGAACT

TACTTTAATGAAACTGATGAATTAATTAATAAAAAAGTGAAGGCAGCATTAGTAAATGGG

TTATGTCCGATTGTTTGTTGTGATGATACTATGCGTCGACGAGTTGCTGGAAAGAAAGTT

CATTGGGTGGTGAGCCGAATTCTCGCTGACCTTCATGGATTGACCAATGACGAAATTTGT

CATGTTACGGTTGCTTATGAACCAAGTTGGGCGATTGGAACAGGCGAGAGTGCTGATCCA

GAACAAGCGGCGGAAGGTTGTTACCTTATTCGGCAAACGATTAGTGATATGTATGGCGAT

GAAGTTGCAAATAACGTTCGAATTCTCTATGGCGGAAGTGTGACAACTTCTAATATCAAT

GCACTAATGGCAAAAAATGATATTGATGGTGTTTTAGTCGGAGCGGCGAGCTTAAATCCA

GAAACATTTTTACAATTAGTTCACCATTAG

以上说明的本发明乳酸菌可以根据常规方法对属于罗伊氏乳杆菌的微生物进行基因分析而获得。例如，通过对多个属于罗伊氏乳杆菌的微生物，研究是否存在与编码脂肪酶(1)～(3)的下述基因(13)～(15)(分别对应于序列表的序列号2、4、6)同源性高的核苷酸序列，从而可以获得目标的本发明乳酸菌。

(13)ATGGTGAAATTGATGACAATACACGAATTAGCAAATAACCCAACGTTAAGCGGCCAAGTA

CGCTTGATTGAAAATATTGTTTATGGTGCGATGGATGGTGAGGCATTACATATGTCGATC

TTAGCACCGTGGACGCAACGTTTCCCGAAACAATATCAAACTGAACCTCGACCATTGATT

GTCTTTGTTCAAGGAAGCTCGTGGCGAACACCAAAAATGGGAGAAGAAATTCCACAACTG

GTTCAATTTGTTCGGGCCGGTTATATTGTAGCGACTGTTCAACACCGTAGTTCAATTGAT

AGCCACCCATTTCCTGCCTTTTTGCAAGATGTTAAGACTGCCATTCGTTTCTTACGGGCC

AATGCGCAAAAATATGCAATTGATCCGCAACAGGTTGCAATTTGGGGGACTTCCTCTGGA

GCCAATGCGGCAATGCTAGTCGGCTTAACGGGTGATGATCCGCGCTATAAAGTTGACCTT

TATCAAGACGAATCGGATGCAGTAGATGCTGTGGTTAGTTGTTTTGCCCCAATGGACGTG

GAGAAGACGTTTGAGTATGATGCTAATGTTCCAGGAAATAAGTTACTGCAATATTGCTTA

TTAGGGCCTGATGTATCAAAGTGGCCAGAAATTGAAAAGCAAATGAGTCCCTTATATCAA

GTCAAAGATGGGCAAAACTACCCACCATTCTTATTGTTCCACGGAGATGCTGATAAAGTT

GTTCCATATGAACAGATGGAAAAAATGTATATGCGGTTGAAGGATAATGGAAATTCTGTT

GAAGCGTACCGGGTTAAGGGTGCGAACCATGAACGAGATTTCTGGAGTCCAACAATTTAT

AATATTGTGCAGAAGTTTCTTGGCGATCAATTTAAATAA

(14)TTGATTTATGTTTTAAAAGATTTATGTAATACTATTGCTGAAGTCTATGGCAAAAGTATT

TTAAAAGGAGTTTTTATCATGAAACATACGCTTAAAGTTGATCAAGTACGTGACGGTTTA

TGGCTAGATTCAGATATTACGTATACGCAAGTTCCTGGATGGCTTGGTAATACAACGCGA

GATTTGAAGCTTTCAGTCATTCGACATTTTCAAACTAATGATGATACACGTTATCCAGTA

ATTTTTTGGTTTGCTGGTGGCGGCTGGATGGATACTGACCACAATGTTCATCTGCCGAAT

TTGGTTGATTTTGCTCGGCATGGTTACATTGTTGTCGGCGTCGAATATCGTGATAGCAAC

AAAGTTCAGTTTCCTGGGCAATTAGAAGATGCTAAGGCTGCTATTCGTTATATGAGAGCT

AATGCCAAGCGCTTCCAAGCTGATCCTAATCGGTTTATTGTGATGGGAGAATCGGCCGGT

GGACATATGGCAAGTATGCTAGGTGTTACTAACGGCCTTAACCAATTTGACAAAGGTGCT

AATTTAGATTACTCCAGTGATGTTCAAGTAGCAGTTCCTTTTTATGGTGTGGTTGATCCC

TTAACCGCTAAAACAGGAAGTGCATCAAACGATTTTGATTTTGTTTACCGTAACTTGCTT

GGTGCTGAGCCTGAAAACGCTCCTGAGCTTGATTCTGCCGCAAATCCCCTCACCTATGTA

AATTCTAATTCTACGCCCTTTCTTATCTTTCATGGGACAGAAGATGTCGTTGTTCCAATT

AAAGATAGTGAAAAGCTTTATGATGCATTAGTTGAAAACAACGTTCCTGCTGAATTATAC

GAAATCGAAGGCGCAAGTCACATGGATGTGAAATTCCTTCAACCACAGGTATTTAAGATT

GTGATGGACTTTTTAGATAAGTATTTAACTCGGTCATAG

(15)ATGGAAATTAAAAGTGTTAACTTAGATCAACCATATTCGTCTCTAGATATTTATCATAGT

AATACTGATAAAGCTTTGCCCGGTCTTGTTATTTTACCAGGAGGCAGTTATAACCAGATC

ATGGAGCGAGATTCTGAACGGGTGGCATTAACGTTTGCAACCCATGCATGGCAAACATTT

GTTGTACGATATCCGGTAGTTGAGCATAAGAATTATGAAGAAGCCAAAATAGCGGTTCAC

CAAGCATTTGAATATATCGTCAACCATGCAGCTGAATTAGATGTTGACGCTGATCGGTTG

GGGATTATTGGCTTTTCTGCAGGAGGCCAAATTGCCGCTGCATATAGTAATGAAAAACTA

ACACACGCTAGATTCGCCGCATTAGGATATCCTGTTATTCAACCCTTGATTGATGAACGT

ATGGGGGTTACAACAGAGAATGTAGCGAAATTAGTAAATCCGCAAACACCACCAACCTTT

ATGTGGGGATCGGCAAAAGATGAACTGACTCCCTTTGTTGATCACCTTCAAGTATATGCA

GATGCGTTAATTAAGAATGATATTCCATATGAATTACATGAGTTTGGCACTGGGGGACAT

GGAATCGCGTTAGCTAACGAATATACTGGTATTGTTAATAATGATCGGGTAGATAATCAT

ATGGGAAAGTGGTTCCCGCTATTTCTTGAGTGGTTAACTGAACTGAATTTAATTTAG

本发明中，所谓的“严格条件”可以列举如下条件：例如在含有6×SSC(1×SSC的组成：0.15M NaCl、0.015M的柠檬酸钠、pH7.0)、0.5％SDS、5×Denhardt、100μg/mL的热变性鲑鱼精子DNA的溶液中，与探针一起在50～65℃的温度下保存一晚使其杂交。

进而，具有运载蛋白基因(4)的本发明乳酸菌，具有编码甘油分解酶亚基的基因(9)～(11)、编码甘油分解酶的基因(32)～(38)、编码醛脱氢酶的基因(39)～(41)、编码甘油酸激酶的基因(42)、编码甘油激酶的基因(43)、编码甘油-3-磷酸脱氢酶的基因(44)、编码磷酸丙糖异构酶的基因(45)～(47)或编码肠道黏附性蛋白质的基因(12)的本发明乳酸菌，也可以与上述同样地获得。

作为上述本发明乳酸菌的代表性菌，可以举出罗伊氏乳杆菌JCM1112T，其为理化学研究所的标准株。

本发明的抗肥胖剂可以通过将上述本发明乳酸菌制成可以口服且以活的状态到达肠道的活菌剂而调制。对于其剂型并无特别限定，例如可以为粉末剂、颗粒、片剂、胶囊剂等固体，凝胶、糊剂等半固体状，悬浮液，糖浆等液体状。这些各剂型可以通过制药领域中公知的方法制得。

配合在上述抗肥胖剂中的本发明乳酸菌可以应用公知的乳酸菌培养方法进行培养。作为该培养方法，可以根据常规方法将本发明乳酸菌进行液体培养，直接利用所得的培养物，或者通过离心分离等方法从该培养物中收集菌体后使用，还可以将培养物冷冻干燥制成粉末状后使用。

作为固体状的本发明抗肥胖剂的通常的制造方法，可以举出将本发明乳酸菌与水、淀粉、微晶纤维素、面粉、砂糖等载体一起配合，制成所需形态的方法。上述载体也是公知的，可以根据使用形态进行适当选择后使用。更具体地说，可以将通过常规方法培养获得的本发明乳酸菌的菌体制成冷冻干燥粉末，将其与砂糖混合，从而调制粉末剂。另外，还可以将本发明乳酸菌的菌体与适当的片剂用载体混合，根据常规方法进行压片，获得片剂。而且，还可以将本发明乳酸菌的湿菌体悬浮在糖浆中制成糖浆剂。调制本发明的抗肥胖剂时，还可以根据需要含有其它成分，例如其它微生物或有效成分、或者甜味料、香料、着色剂等。

如此获得的抗肥胖剂的给予量可以考虑对象的身体状况、例如健康状态、体重、年龄、病史、所使用的其它成分等适当地决定，一般来说，作为本发明乳酸菌的菌数，一个成人大致为10⁸～10⁹CFU/天左右。

使用本发明乳酸菌调制抗肥胖饮食品时，可以利用目前公知的乳酸菌的培养方法制成可以口服摄取的发酵食品。具体地说，可以制成酸奶等发酵乳、乳酸菌饮料、发酵香肠等食品，这些食品的制造可以通过使部分或全部所用乳酸菌采用本发明乳酸菌来进行。另外，还可以形成较多地含有本发明乳酸菌的形态，制成健康食品和功能性食品。在调制该抗肥胖食品时，当然并非仅含有本发明乳酸菌，还可以含有其它的乳酸菌，另外，还可以添加食品添加剂或调味品等。

本发明乳酸菌如后述实施例所示，具有显著的体重增加抑制效果(瘦身效果)，认为其理由如下所示。

即，如图1所示，本发明乳酸菌通过3个脂肪酶(脂肪酶(1)～(3))的作用，将消化道内的脂肪成分分解为甘油和脂肪酸。然后，所分解的甘油被本发明乳酸菌所具有的运载蛋白(PduF；(4)的核苷酸编码)摄入到菌体内，进而被菌体内的甘油分解酶基因(PduCDE；由亚基(5)～(7)构成)代谢，产生罗伊氏素或转变为本菌自身的能量源。

另一方面，脂肪酸作为本菌的菌体成分被利用，而不会被吸收入生物体内。而且，(8)的肽具有本发明乳酸菌固定在人或哺乳动物肠道的功能，使本发明乳酸菌可以稳定地在肠道内存在一定期间。

如上所述，本发明乳酸菌由于稳定地存在于肠道内、积极地分解脂肪、且利用或进一步分解其代谢物，因此可以抑制脂质从肠道被吸收入体内、即便摄取通常的饮食也可以预防肥胖、维持及提高瘦身效果。

另外，本发明的一个方式包括本发明乳酸菌用于预防或治疗肥胖症的应用，还包括本发明乳酸菌用于制造抗肥胖剂的应用。

进而，本发明的又一个方式包括以向肥胖症患者给予本发明乳酸菌为特征的肥胖症治疗方法，还包括以给予本发明抗肥胖剂为特征的肥胖症治疗方法。

实施例

以下举出实施例，更加详细地说明本发明，但本发明并不局限于这些实施例。

实施例1

罗伊氏乳杆菌JCM1112T的抗肥胖效果试验：

通过下述材料和方法研究罗伊氏乳杆菌JCM1112T的抗肥胖效果。

使用材料和试验方法：

(1)使用的实验动物

使用体重约180～200g的SPF级Wistar系大鼠(8周龄-雄；日本SLC株式会社)，从放入实验室开始设置7天的驯化期，开始实验。饲养环境设定为温度22±1℃、湿度55±5％、照明时间12小时(8点～20点)，大鼠为1只/笼，利用给水瓶使其自由摄取灭菌蒸馏水、使用给饵器使其自由摄取大鼠用放射线灭菌固体样品^*(CE-2，CLEA Japan Inc.)。所使用的饲养器具均使用通过高压蒸汽灭菌器灭菌的器具。

^*饲养繁殖用(粗脂肪4.6％)

(2)待测菌液的调制

作为待测菌，使用罗伊氏乳杆菌JCM1112T(理化学研究所的标准菌、从该所购买)和鼠李糖乳杆菌(L.rhamnosus)ATCC53103(GG株)。这些待测菌接种于MRS液体培养基(Oxid)，在37℃下培养一晚，制成预培养液。将该预培养液添加到新鲜的MRS液体培养基中达到1％量，在37℃下培养18小时，将其作为待测菌液。

(3)待测菌液的给予

将上述实验动物分为各待测菌给予组和对照组(1组各5只)，对待测菌给予组，将上述待测菌液通过口服给予。待测菌的给予使用探针强制进行。给予菌液在使用时调制，给予菌量为每只大鼠10⁹CFU。另外，对对照组给予相同容量的PBS。

(4)体重测定和一般症状的观察

使用体重计每天测定实验动物的体重。每天均观察一般症状。对每个个体记录症状，用表现个体数来表示观察到显著变化的症状项目。

(5)结果

图2示意出分别给予上述乳杆菌属细菌的大鼠组和未给予的大鼠组的体重随时间增加的状况。由该图可知，与未给予组相比，罗伊氏乳杆菌JCM1112T给予组显著抑制了体重的增加，其程度大于鼠李糖乳杆菌ATCC53103给予组。

另外，实验动物的健康状态良好是由良好的体重增加的进展来显示的同时，由各组大鼠的诊察来确认的。

实施例2

罗伊氏乳杆菌JCM1112T的基因组解析：

使用下述药品，通过以下方法从罗伊氏乳杆菌JCM1112T中获取DNA，进行基因组解析。

DNA获取方法：

(药品)

核裂解液(Wizard基因组DNA纯化试剂盒；Promega公司生产)

生理盐水

50mM EDTA(pH7.0)

50mg/ml溶菌酶溶液(在使用当日，用TE缓冲液、0.25MTris-HCl(pH8.0)或10mM Tris-HCl(pH8.0)/10mMEDTA/0.5％SDS溶解规定量的溶菌酶进行调制)

2mg/ml EDTA

苯酚·氯仿·异戊醇(25∶24∶1)混合液(以下简称为“PCI”)

氯仿·异戊醇(24∶1)混合液(以下简称为“CIA”)

99％乙醇

70％乙醇

TE缓冲液

(方法)

1.在静置条件、37℃下培养罗伊氏乳杆菌JCM1112T 24小时，在3500rpm下离心分离所得培养液(50ml)15分钟后，利用生理盐水悬浮。

2.在3500rpm下离心分离上述1所得的悬浮液15分钟，舍去上清，利用5ml 50mM EDTA悬浮。

3.在上述2所得的悬浮液中加入200μl 50mg/ml溶菌酶溶液，在37℃下培养60分钟。(此时使用空气培养箱时，培养时间为2～3小时)

4.培养后，在3500rpm下离心分离15分钟，舍去上清。

5.在4所得的沉淀物中加入核裂解液5ml，在80℃下培养10分钟。

6.加入1μl 2mg/ml RNase A，在37℃下培养45分钟。

7.加入10ml PCI并混合，在3500rpm下离心分离15分钟。

8.将上清移至新的试管中，再加入10ml PCI并混合。

9.共增加3次同样的操作。(直至变得不能识别蛋白质层)

10.加入10ml CIA，缓缓混合后，在3500rpm下离心分离15分钟。(除去苯酚)

11.将上清移至新的试管中，进行乙醇沉淀。

12.将11所得的沉淀物溶解在1ml TE缓冲液中，获得乳酸菌DNA。

基因组解析方法：

对于如上获得的DNA进行结构基因预测和解析。联合使用GENOMEGAMBLER(Sakiyama，T.Takami，H.Ogasawara，N.Kuhara.S.Kozuki，T.Doga，K.Ohyama，A.Horikoshi，K.Anauromated system for genome analysis to support microbial whole-genome shotgun sequencing.Biosci.Biotechnol.Bichem.64：670-673.2000.)、GLIMMER 2.0(Salzberg，SL.Delcher，AL.Kasif，S.and White，O.Microbial gene identification usinginterpolated Markov models.Nucleic.Acid.Res.26：544-548.1998.)和BLAST program blastp(Altschul，SF.Gish，W.Miller，W.Myers，EW.and Lipman，DJ.Basic local alignment searchtool.J.Mol.Biol.215：403-410.1990)的结果来进行该结构基因预测等。

另外，域结构的解析使用INTERPRO(Mulder，NJ.Apweiler，R.Attwood，TK.Bairoch，A.Barrell，D.Bateman，A.Binns，D.Biswas，M.Bradley，P.Bork，P.Bucher，P.Copley，PR.Courcelle，E.Das，U.Durbin，R.Falquet，L.Fleischmann，W.Griffiths-Johns，S.Haft，D.Harte，N.Hulo，N.Kahn，D.Silventoinen，V.Orchard，SE.Pagni，M.Peyruc，D，，Ponting，CP.Selengut，JD.Servant，F.Sigrist，CJ.Vaughan，R.andZdobnov，EM.The InterPro Database，2003 brings increasedcoverage and new features.Nucleic.Acids.Res.31：315-318.2003，；http://www.ebj.ac.uk/interpro)进行；分子系统树的制作中使用了CLUSTALW(Thompson，JD.Higgins，DG.and Gibson，TJ.CLUSTAL W：improving the sensitivity of progressivemultiple sequence alignment through sequence weighting，position-specific gap penalties and weight matrix choice.Nucleic.Acids.Res.22：4673-4680.1994.http://clustalw.Genome.ad.jp/)。

进而，所使用的DNA和氨基酸序列由National Center ofBiological Information(NCBI，http://www.ncibi.nlm.nih.gov)和KEGG database(Ogata，H.Goto，S.Sato，K.Fujibuchi，W.Bono，H.and Kanehisa，M.KEGG：Kyoto Encuclopedia ofGenes and Genomes.Nucleic，Acids.Res.27：29-34.1999.http://www.genome.ad.jp/kegg/kegg2.html)获得，乳杆菌属细菌的序列草图由DOE JOINT GENOME INSTITUTE(JGI；http://www.jgi.doe.gov/JGI_microbial/html/index.html)获得。

根据以上信息可知，(13)～(15)所示的基因分别为编码脂肪酶的基因、(4)所示的基因为编码运载蛋白的基因、(9)、(10)和(11)所示的基因为编码甘油分解酶的基因、(12)所示的基因为黏附性基因。另外可知，(32)～(38)所示的基因为编码甘油分解酶的基因、(39)～(41)所示的基因为编码醛脱氢酶的基因、基因(42)所示的基因为编码甘油酸激酶的基因、(43)所示的基因为编码甘油激酶的基因、(44)所示的基因为编码甘油-3-磷酸脱氢酶的基因、(45)～(47)所示基因为编码磷酸丙糖异构酶的基因。

实施例3

甘油分解基因的扩增：

将实施例2中精制的DNA作为模板，将以下核苷酸序列作为引物，使用下述反应液进行PCR。PCR条件也示于下。

(使用的引物)

pduCDE(F)：

CACCATGAAACGTCAAAAACGATTT

pduCDE(R)：

AAAAGCTTAGTTATCGCCCTTTAGC

(PCR反应液)

模板DNA：1μl

KOD-plus：1μl

10×KOD-plus缓冲液：5μl

dNTP(各2mM)：5μl

引物(20mm)：各1μl

MgSO4(25mm)：2μl

去离子水(D.W.)：34μl

(PCR条件)

(1)在94℃下保持3分钟。

(2)在94℃下保持15秒钟。

(3)在56℃(Tm)下保持30秒钟。

(4)在68℃下保持3分30秒钟。

(5)将(2)～(4)进行30个循环。

(6)在4℃下保存

实施例4

罗伊氏乳杆菌来源的脂肪酶基因和黏附性基因的扩增：

除了将引物换成下述引物之外，与实施例3同样地进行PCR，扩增脂肪酶基因和黏附性基因。

(使用的引物)

脂肪酶(1)

5’-ATGGTGAAATTGATGACAAT

5’-TTATTTAAATTGATCGCCAA

脂肪酶(2)

5’-TTGATTTATGTTTTAAAAGA

5’-CTATGACCGAGTTAAATACT

脂肪酶(3)

5’-ATGGAAATTAAAAGTGTTAA

5’-CTAAATTAAATTCAGTTCAG

黏附性基因

5’-ATGTTCGGTCACGATGGCCG

5’-TCAAATTTCAGAAGGATCAT

产业实用性

由使用了作为本发明乳酸菌代表的罗伊氏乳杆菌JCM1112T的上述实施例的结果可知，通过该微生物的给予，可以抑制体重的增加，而不损害实验动物的健康、尤其是对营养摄取没有限制。

因此，仅摄取利用了本发明乳酸菌的抗肥胖剂和抗肥胖饮食品，不需要特殊治疗、处置即可预防肥胖、获得瘦身效果。

通过利用公知的方法将编码由本发明乳酸菌发现的甘油分解酶亚基的基因和编码肠道黏附性蛋白质的基因整合在其它乳酸菌中，可以获得甘油分解性高的乳酸菌和在肠道内的停留时间长的乳酸菌，可以有效地用于其它有用的乳酸菌改性中。

序列表

<110>学校法人麻布兽医学园(School Corporation，Azabu Veterinary Medicine

Educational Institution)

<120>抗肥胖剂及抗肥胖食品

<130>PF-060017-WO

<150>JP2005-215895

<151>2005-07-26

<160>48

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Met Gly Pro Asn Asp Pro Lys Pro Ser Val Lys Val Glu Asn Gly Lys

35 40 45

Ile Val Glu Met Asp Gly Lys Lys Leu Glu Asp Phe Asp Leu Ile Asp

50 55 60

Leu Tyr Ile Ala Lys Tyr Gly Ile Asn Ile Asp Asn Val Glu Lys Val

65 70 75 80

Met Asn Met Asp Ser Thr Lys Ile Ala Arg Met Leu Val Asp Pro Asn

85 90 95

Val Ser Arg Asp Glu Ile Ile Glu Ile Thr Ser Ala Leu Thr Pro Ala

100 105 110

Lys Ala Glu Glu Ile Ile Ser Lys Leu Asp Phe Gly Glu Met Ile Met

115 120 125

Ala Val Lys Lys Met Arg Pro Arg Arg Lys Pro Asp Asn Gln Cys His

130 135 140

Val Thr Asn Thr Val Asp Asn Pro Val Gln Ile Ala Ala Asp Ala Ala

145 150 155 160

Asp Ala Ala Leu Arg Gly Phe Pro Glu Gln Glu Thr Thr Thr Ala Val

165 170 175

Ala Arg Tyr Ala Pro Phe Asn Ala Ile Ser Ile Leu Ile Gly Ala Gln

180 185 190

Thr Gly Arg Pro Gly Val Leu Thr Gln Cys Ser Val Glu Glu Ala Thr

195 200 205

Glu Leu Gln Leu Gly Met Arg Gly Phe Thr Ala Tyr Ala Glu Thr Ile

210 215 220

Ser Val Tyr Gly Thr Asp Arg Val Phe Thr Asp Gly Asp Asp Thr Pro

225 230 235 240

Trp Ser Lys Gly Phe Leu Ala Ser Cys Tyr Ala Ser Arg Gly Leu Lys

245 250 255

Met Arg Phe Thr Ser Gly Ala Gly Ser Glu Val Leu Met Gly Tyr Pro

260 265 270

Glu Gly Lys Ser Met Leu Tyr Leu Glu Ala Arg Cys Ile Leu Leu Thr

275 280 285

Lys Ala Ser Gly Val Gln Gly Leu Gln Asn Gly Ala Val Ser Cys Ile

290 295 300

Glu Ile Pro Gly Ala Val Pro Asn Gly Ile Arg Glu Val Leu Gly Glu

305 310 315 320

Asn Leu Leu Cys Met Met Cys Asp Ile Glu Cys Ala Ser Gly Cys Asp

325 330 335

Gln Ala Tyr Ser His Ser Asp Met Arg Arg Thr Glu Arg Phe Ile Gly

340 345 350

Gln Phe Ile Ala Gly Thr Asp Tyr Ile Asn Ser Gly Tyr Ser Ser Thr

355 360 365

Pro Asn Tyr Asp Asn Thr Phe Ala Gly Ser Asn Thr Asp Ala Met Asp

370 375 380

Tyr Asp Asp Met Tyr Val Met Glu Arg Asp Leu Gly Gln Tyr Tyr Gly

385 390 395 400

Ile His Pro Val Lys Glu Glu Thr Ile Ile Lys Ala Arg Asn Lys Ala

405 410 415

Ala Lys Ala Leu Gln Ala Val Phe Glu Asp Leu Gly Leu Pro Lys Ile

420 425 430

Thr Asp Glu Glu Val Glu Ala Ala Thr Tyr Ala Asn Thr His Asp Asp

435 440 445

Met Pro Lys Arg Asp Met Val Ala Asp Met Lys Ala Ala Gln Asp Met

450 455 460

Met Asp Arg Gly Ile Thr Ala Ile Asp Ile Ile Lys Ala Leu Tyr Asn

465 470 475 480

His Gly Phe Lys Asp Val Ala Glu Ala Ile Leu Asn Leu Gln Lys Gln

485 490 495

Lys Val Val Gly Asp Tyr Leu Gln Thr Ser Ser Ile Phe Asp Lys Asp

500 505 510

Trp Asn Val Thr Ser Ala Val Asn Asp Gly Asn Asp Tyr Gln Gly Pro

515 520 525

Gly Thr Gly Tyr Arg Leu Tyr Glu Asp Lys Glu Glu Trp Asp Arg Ile

530 535 540

Lys Asp Leu Pro Phe Ala Leu Asp Pro Glu His Leu Glu Leu

545 550 555

<210>10

<211>1677

<212>DNA

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>10

atgaaacgtc aaaaacgatt tgaagaacta gaaaaacggc caattcatca agatacattt 60

gttaaagaat ggccagaaga aggtttcgtt gcaatgatgg ggcctaatga ccctaagcct 120

agtgtaaaag ttgaaaatgg caagatcgta gagatggatg gtaaaaagct cgaagatttt 180

gatttgattg acttgtacat tgctaagtat ggaatcaata ttgacaacgt tgaaaaagtt 240

atgaatatgg attctaccaa gattgcacgg atgcttgttg atcctaatgt ttctcgtgat 300

gaaattattg aaattacatc agctttgact cctgctaagg ctgaagagat catcagtaag 360

cttgattttg gtgaaatgat tatggctgtc aagaagatgc gcccacgtcg taagcctgac 420

aaccagtgtc acgttaccaa tactgttgat aacccagttc aaattgctgc tgatgctgct 480

gatgccgctc ttcgtggatt tccagaacaa gaaaccacga cagctgtggc acgttatgca 540

ccattcaatg ctatttcaat tttaattggt gcacaaacag gtcgccctgg tgtattgaca 600

caatgttctg ttgaagaagc tactgaattg caattaggta tgcgtggttt taccgcatat 660

gctgaaacca tttcagttta cggtactgat cgtgtattta ccgatggtga tgatactcca 720

tggtctaaag gcttcttggc atcttgttat gcatcacgtg gtttgaagat gcgatttact 780

tcaggtgccg gttcagaagt tttgatgggt tatccagaag gtaagtcaat gctttacctt 840

gaagcgcgtt gtattttact tactaaggct tcaggtgttc aaggacttca aaatggtgcc 900

gtaagttgta ttgaaattcc tggtgctgtt cctaatggta ttcgtgaagt tctcggtgaa 960

aacttgttat gtatgatgtg tgacatcgaa tgtgcttctg gttgtgacca agcatactca 1020

cactccgata tgcggcggac tgaacggttt attggtcaat ttattgccgg tactgattat 1080

attaactctg gttactcatc aactcctaac tacgataata ccttcgctgg ttcaaacact 1140

gatgctatgg actacgatga tatgtatgtt atggaacgtg acttgggtca atattatggt 1200

attcaccctg ttaaggaaga aaccattatt aaggcacgta ataaggccgc taaagccctt 1260

caagcagtat ttgaagatct tggattacca aagattactg atgaagaggt cgaagcagca 1320

acgtatgcta acacccatga tgacatgcca aagcgggata tggttgcaga tatgaaggct 1380

gctcaagata tgatggatcg tggaattact gctattgata ttatcaaggc attgtacaac 1440

cacggattta aagatgtcgc tgaagcaatt ttgaaccttc aaaaacaaaa agttgttggt 1500

gattaccttc aaacatcttc tatttttgat aaagattgga acgtcacttc tgctgttaac 1560

gacggaaatg attatcaagg accaggtact ggataccgtc tatatgaaga caaggaagaa 1620

tgggatcgga ttaaagactt accattcgcc cttgatccag aacatttgga actgtag 1677

<210>11

<211>236

<212>PRT

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>11

Met Ala Asp Ile Asp Glu Asn Leu Leu Arg Lys Ile Val Lys Glu Val

1 5 10 15

Leu Ser Glu Thr Asn Gln Ile Asp Thr Lys Ile Asp Phe Asp Lys Ser

20 25 30

Asn Asp Ser Thr Ala Thr Ala Thr Gln Glu Val Gln Gln Pro Asn Ser

35 40 45

Lys Ala Val Pro Glu Lys Lys Leu Asp Trp Phe Gln Pro Val Gly Glu

50 55 60

Ala Lys Pro Gly Tyr Ser Lys Asp Glu Val Val Ile Ala Val Gly Pro

65 70 75 80

Ala Phe Ala Thr Val Leu Asp Lys Thr Glu Thr Gly Ile Pro His Lys

85 90 95

Glu Val Leu Arg Gln Val Ile Ala Gly Ile Glu Glu Glu Gly Leu Lys

100 105 110

Ala Arg Val Val Lys Val Tyr Arg Ser Ser Asp Val Ala Phe Cys Ala

115 120 125

Val Gln Gly Asp Hi s Leu Ser Gly Ser GlyIle Ala Ile Gly Ile Gln

130 135 140

Ser Lys Gly Thr Thr Val Ile His Gln Lys Asp Gln Asp Pro Leu Gly

145 150 155 160

Asn Leu Glu Leu Phe Pro Gln Ala Pro Val Leu Thr Pro Glu Thr Tyr

165 170 175

Arg Ala Ile Gly Lys Asn Ala Ala Met Tyr Ala Lys Gly Glu Ser Pro

180 185 190

Glu Pro Val Pro Ala Lys Asn Asp Gln Leu Ala Arg Ile His Tyr Gln

195 200 205

Ala Ile Ser Ala Ile Met His Ile Arg Glu Thr His Gln Val Val Val

210 215 220

Gly Lys Pro Glu Glu Glu Ile Lys Val Thr Phe Asp

225 230 235

<210>12

<211>711

<212>DNA

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>12

atggctgata ttgatgaaaa cttattacgt aaaatcgtta aagaagtttt aagcgaaact 60

aatcaaatcg atactaagat tgactttgat aaaagtaatg atagtactgc aacagcaact 120

caagaggtgc aacaaccaaa tagtaaagct gttccagaaa agaaacttga ctggttccaa 180

ccagttggag aagcaaaacc tggatattct aaggatgaag ttgtaattgc agtcggtcct 240

gcattcgcaa ctgttcttga taagacagaa actggtattc ctcataaaga agtgcttcgt 300

caagttattg ctggtattga agaagaaggg cttaaggcgc gggtagttaa agtttaccgg 360

agttcagatg tagcattctg tgctgtccaa ggtgatcacc tttctggttc aggaattgct 420

attggtatcc aatcaaaagg gacgacagtt attcaccaaa aggatcaaga ccctcttggt 480

aaccttgagt tattcccaca agcgccagta cttactcccg aaacttatcg tgcaattggt 540

aagaatgccg ctatgtatgc taagggtgaa tctccagaac cagttccagc taaaaacgat 600

caacttgctc gtattcacta tcaagctatt tcagcaatta tgcatattcg tgaaactcac 660

caagttgttg ttggtaagcc tgaagaagaa attaaggtta cgtttgatta a 711

<210>13

<211>171

<212>PRT

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>13

Met Ser Glu Val Asp Asp Leu Val Ala Lys Ile Met Ala Gln Met Gly

1 5 10 15

Asn Ser Ser Ser Ala Asn Ser Ser Thr Gly Thr Ser Thr Ala Ser Thr

20 25 30

Ser Lys Glu Met Thr Ala Asp Asp Tyr Pro Leu Tyr Gln Lys His Arg

35 40 45

Asp Leu Val Lys Thr Pro Lys Gly His Asn Leu Asp Asp Ile Asn Leu

50 55 60

Gln Lys Val Val Asn Asn Gln Val Asp Pro Lys Glu Leu Arg Ile Thr

65 70 75 80

Pro Glu Ala Leu Lys Leu Gln Gly Glu Ile Ala Ala Asn Ala Gly Arg

85 90 95

Pro Ala Ile Gln Lys Asn Leu Gln Arg Ala Ala Glu Leu Thr Arg Val

100 105 110

Pro Asp Glu Arg Val Leu Glu Met Tyr Asp Ala Leu Arg Pro Phe Arg

115 120 125

Ser Thr Lys Gln Glu Leu Leu Asn Ile Ala Lys Glu Leu Arg Asp Lys

130 135 140

Tyr Asp Ala Asn Val Cys Ala Ala Trp Phe Glu Glu Ala Ala Asp Tyr

145 150 155 160

Tyr Glu Ser Arg Lys Lys Leu Lys Gly Asp Asn

165 170

<210>14

<211>516

<212>DNA

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>14

atgagtgaag ttgatgattt agtagcaaag atcatggctc agatgggaaa cagttcatct 60

gctaatagct ctacaggtac ttcaactgca agtactagta aggaaatgac agcagatgat 120

tacccacttt atcaaaagca ccgtgattta gtaaaaacac caaaaggaca caatcttgat 180

gacatcaatt tacaaaaagt agtaaataat caagttgatc ctaaggaatt acggattaca 240

ccagaagcat tgaaacttca aggtgaaatt gcagctaatg ctggccgtcc agctattcaa 300

aagaatcttc aacgagctgc agaattaaca cgagtacctg acgaacgggt tcttgaaatg 360

tatgatgcat tgcgtccttt ccgttcaact aagcaagaat tattgaacat tgcaaaggaa 420

ttacgggaca agtatgacgc taatgtttgc gcagcatggt ttgaagaagc tgctgattat 480

tatgaaagtc gtaagaagct aaagggcgat aactaa 516

<210>15

<211>184

<212>PRT

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>15

Met Phe Gly His Asp Gly Arg Ile Val Thr Lys Val Tyr Gln Trp Ala

1 5 10 15

Gly Thr Tyr Tyr Tyr Phe Asp Pro Asn Thr Tyr Leu Arg Val Asp Asn

20 25 30

Asp Tyr Arg Gln Ser Gln Trp Gly Asp Trp Tyr Met Phe Gly Pro Asp

35 40 45

Gly Arg Ile Val Thr Gly Leu Lys Glu Trp Tyr Gly Ser Tyr Tyr Tyr

50 55 60

Phe Asp Pro Thr Thr Tyr Leu Lys Val Thr Asn Lys Trp Ile Asp Asn

65 70 75 80

Lys Tyr Phe Gly Pro Ala Gly Gln Gln Ala Ile Ser Arg Phe Glu Arg

85 90 95

Leu Asp Asn Lys Tyr Tyr Tyr Phe Asp Ala Asn Gly Ala Val Leu Asn

100 105 110

Ile His Asp Gln Phe Lys Asn Ile Asp Asn His Thr Tyr Tyr Phe Gly

115 120 125

Ala Asp Gly Ala Cys Tyr Thr Ser Gln Phe Leu Asn Lys Asp Gly Lys

130 135 140

Gln Tyr Tyr Phe Asp Asn Asp Gly Ile Met Leu Thr Asp Gln Glu Lys

145 150 155 160

Ile Ile Asp Gly Lys Phe Tyr His Phe Asn Val Asn Gly Glu Ala Ile

165 170 175

Gln Val Asn Asp Pro Ser Glu Ile

180

<210>16

<211>555

<212>DNA

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>16

atgttcggtc acgatggccg cattgttact aaagtttacc aatgggctgg cacgtattac 60

tactttgatc cgaatactta tttgcgagta gataatgatt accgtcaatc tcagtggggc 120

gattggtata tgtttggccc agatggtcgt atcgttacag ggttaaagga atggtacggt 180

agttattatt actttgatcc gacgacttac ttaaaagtaa ctaataagtg gatagataat 240

aagtactttg gtccagctgg tcagcaagct atttcacgct ttgagagact tgataataag 300

tattactatt tcgatgctaa tggggcagtt cttaatatcc atgatcaatt taagaatatt 360

gataaccaca cttattactt tggagctgat ggtgcttgtt ataccagtca attcttaaat 420

aaggatggta aacagtatta tttcgataat gatggaatta tgctcactga tcaagagaag 480

atcattgacg gtaaattcta tcatttcaat gttaatggtg aagcaatcca agtaaatgat 540

ccttctgaaa tttga 555

<210>17

<211>342

<212>PRT

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>17

Met Lys Ala Ala Val Ile Asn Asp Pro Val Asp Gly Phe Val Thr Val

1 5 10 15

Lys Asp Val Gln Leu Arg Asp Leu Lys Pro Gly Glu Ala Leu Val Asp

20 25 30

Met Glu Tyr Cys Gly Leu Cys His Thr Asp Leu His Val Ala Ala Gly

35 40 45

Asp Phe Gly Lys Lys Pro Gly Arg Ile Ile Gly His Glu Gly Val Gly

50 55 60

Arg Val Ser Lys Val Ala Pro Gly Val Thr Ser Leu Lys Val Gly Asp

65 70 75 80

Arg Val Ser Ile Ala Trp Phe Phe Lys Gly Cys Gly His Cys Glu Tyr

85 90 95

Cys Leu Thr Gly Arg Glu Thr Leu Cys Arg Asn Val Leu Asn Ala Gly

100 105 110

Tyr Thr Ala Asp Gly Ala Met Ala Glu Gln Cys Ile Val Pro Ala Asp

115 120 125

Tyr Ala Val Lys Val Pro Glu Gly Leu Asp Pro Val Glu Ala Thr Ser

130 135 140

Leu Thr Cys Ala Gly Val Thr Met Tyr Lys Ala Leu Lys Val Ala Asp

145 150 155 160

Ile Lys Pro Gly Gln Trp Val Ser Ile Val Gly Ala Gly Gly Leu Gly

165 170 175

Asn Leu Gly Ile Gln Leu Ala His Asn Val Phe Gly Ala His Val Ile

180 185 190

Ala Val Asp Gly Asn Pro Asp Lys Leu Glu Ala Ala Lys Lys Asn Gly

195 200 205

Ala Glu Ile Leu Ile Asn Arg His Asp Gly Asp Val Asp Lys Gln Ile

210 215 220

Gln Glu Lys Val Gly Gly Val His Ala Ala Val Val Thr Ala Val Ser

225 230 235 240

Ala Ser Ala Phe Asp Gln Ala Val Asp Ser Leu Arg Pro Asp Gly Lys

245 250 255

Leu Val Ala Val Ala Leu Pro Gln Gly Asp Met Lys Leu Asn Ile Ala

260 265 270

Lys Thr Val Leu Asp Gly Ile Ile Val Ala Gly Ser Leu Val Gly Thr

275 280 285

Arg Gln Asp Leu Ala Glu Cys Phe Gln Phe Gly Ala Glu Gly Lys Val

290 295 300

His Pro Ile Val Lys Thr Arg Lys Leu Ser Glu Ile Asn Asp Met Ile

305 310 315 320

Gln Glu Leu Lys Asp Asn Lys Val Val Gly Arg Asn Val Val Asp Phe

325 330 335

Val His Asn Asp Asn Asp

340

<210>18

<211>1029

<212>DNA

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>18

atgaaagctg ctgttattaa tgatccagta gacggttttg ttactgttaa agatgttcaa 60

cttcgggatt tgaagcccgg tgaagcttta gttgacatgg aatattgtgg tctttgtcac 120

actgatctac acgttgctgc tggggacttt ggtaagaagc ccggtcgtat tatcggtcac 180

gaaggggttg gtcgtgtatc taaggttgcc cctggcgtta cttccttgaa agttggcgac 240

cgtgtatcaa ttgcatggtt cttcaagggc tgtggacact gtgaatattg tttaactggt 300

cgtgaaactc tttgtcggaa cgttcttaat gcgggttaca ctgctgacgg tgcaatggct 360

gaacaatgta tcgtaccagc tgactacgct gttaaggttc cagaaggtct tgatcctgtt 420

gaagctactt cattaacttg tgctggtgtt acgatgtaca aggcattaaa ggttgctgac 480

atcaagccag gtcaatgggt atcaatcgtt ggtgctggtg gtttaggtaa cttgggtatt 540

caacttgctc acaacgtatt tggtgctcat gttatcgctg ttgatggtaa tcctgataag 600

cttgaagccg ctaagaagaa tggtgctgaa attttaatta accgtcatga cggtgatgtt 660

gataagcaaa ttcaagaaaa ggttggcggt gttcacgctg ctgtagtaac agctgtttct 720

gcctctgcat tcgaccaagc agttgattca cttcgcccag atggtaagct tgttgccgtt 780

gcgcttccac aaggtgacat gaagcttaac attgctaaga ctgttcttga tggtatcatt 840

gttgctggtt cattagttgg tacccgtcaa gacttagctg aatgtttcca atttggtgca 900

gaaggtaagg ttcacccaat tgttaagact cgtaagttaa gcgaaattaa tgatatgatc 960

caagaactta aggataacaa ggttgttggt cggaatgttg ttgattttgt tcacaacgat 1020

aacgactaa 1029

<210>19

<211>348

<212>PRT

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>19

Met Glu Lys Arg Glu Asn Ala Ile Pro Lys Thr Met Lys Ala Trp Ala

1 5 10 15

Val Thr Thr Pro Gly Pro Ile Asp Gly Lys Glu Ser Pro Ile Glu Phe

20 25 30

Thr Glu Lys Pro Val Pro Thr Pro Lys Arg Gly Glu Val Leu Val Lys

35 40 45

Val Ile Thr Cys Gly Val Cys His Thr Asp Leu His Val Thr Glu Gly

50 55 60

Asp Leu Pro Val His His Glu His Val Thr Pro Gly His Glu Ile Val

65 70 75 80

Gly Lys Val Val Gly Phe Gly Pro Glu Thr Gln Arg Phe Lys Phe Gly

85 90 95

Glu Arg Ile Gly Ile Pro Trp Phe Arg His Ala Cys Gly Val Cys Lys

100 105 110

Phe Cys Arg Ser Gly His Glu Asn Leu Cys Pro His Ser Leu Tyr Thr

115 120 125

Gly Trp Asp His Asp Gly Gly Tyr Ala Glu Tyr Val Thr Val Pro Glu

130 135 140

Gly Phe Ala Tyr Arg Leu Pro Glu Lys Phe Asp Ser Leu Glu Ala Ala

145 150 155 160

Pro Leu Leu Cys Ala Gly Ile Ile Gly Tyr Arg Ala Phe Glu Arg Ala

165 170 175

Asn Val Pro Ala Gly Gly Arg Leu Gly Leu Tyr Gly Phe Gly Gly Ser

180 185 190

Ala His Ile Thr Ala Gln Ile Ala Leu Ala Gln Gly Ile Glu Val His

195 200 205

Val Phe Thr Arg Gly Glu Asp Ala Lys Lys Phe Ala Leu Glu Leu Gly

210 215 220

Cys Ala Ser Val Gln Gly Ser Tyr Asp Pro Ala Pro Val Pro Leu Asp

225 230 235 240

Ser Ser Ile Ile Phe Ala Pro Val Gly Asp Met Val Leu Pro Ala Leu

245 250 255

Ala Ser Leu Val Pro Gly Gly Thr Leu Ala Leu Ala Gly Ile His Met

260 265 270

Thr Asp Ile Pro Thr Met Asn Tyr Gln Lys Glu Ile Phe His Glu Lys

275 280 285

Thr Leu Thr Ser Val Glu Ser Asn Thr Arg Arg Asp Gly Glu Glu Phe

290 295 300

Leu Thr Leu Ala Asp Arg Leu Asn Ile His Pro Glu Val His Glu Tyr

305 310 315 320

Pro Leu Ala Lys Ala Asp Glu Ala Leu Arg Tyr Val Lys His Gly Asp

325 330 335

Ile Lys Gly Ala Cys Val Leu Arg Val Ser Glu Asp

340 345

<210>20

<211>1047

<212>DNA

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>20

atggaaaaac gcgaaaatgc tattccgaaa acaatgaagg cttgggcagt cacaactcct 60

gggccgattg atggtaagga atcaccaatc gaatttaccg aaaagcctgt gccgactcct 120

aaacggggag aagtccttgt taaggtaata acgtgtggag tatgtcatac ggacttgcac 180

gtgactgaag gagacttgcc ggttcaccac gaacacgtta ctcctggtca tgaaattgtt 240

ggtaaagttg tcggctttgg accagagaca caacgattta agtttggtga gcgaattggg 300

attccatggt ttcggcatgc ttgtggtgta tgcaagtttt gccgatcagg tcatgagaat 360

ctctgtcctc attcacttta taccggttgg gatcatgatg gcggttatgc agaatatgtc 420

acagttccag aaggatttgc atatcggctt ccagaaaagt ttgattccct agaggcagct 480

ccgttattat gtgcagggat tattggttat cgggcctttg aacgtgccaa tgttccggct 540

ggcggtcgcc taggattata tggcttcggt ggttcagctc atattacagc tcaaattgca 600

cttgctcagg gaattgaagt gcatgtcttt acgcgtggtg aggatgccaa gaaattcgcc 660

ctagaattag gttgtgcttc tgttcagggc tcctatgacc cagcaccagt tcctttggat 720

tcatcaatca tttttgcgcc ggttggtgat atggtcttgc cggctttagc tagtttagtt 780

ccagggggga cattagcatt agccggtatt catatgactg atattccaac aatgaattac 840

caaaaagaaa tattccacga aaagacatta acgagtgttg agagtaatac tcgtcgtgat 900

ggggaagaat tcttaacatt agctgatcgt cttaatatcc atcctgaagt ccacgaatat 960

cccctagcaa aggctgacga agcattacgc tatgttaagc acggtgatat taagggagct 1020

tgtgtattac gtgttagtga ggactaa 1047

<210>21

<211>486

<212>PRT

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>21

Met Gln Ile Lys Ala Ala Leu Ala Thr Lys Pro Asn Ala Asp Leu Glu

1 5 10 15

Ile Gln Thr Val Glu Leu Asp Glu Pro Lys Glu Asn Glu Val Leu Ile

20 25 30

Lys Ile Ala Ser Thr Gly Phe Cys His Thr Asp Ile Val Gly Arg Ser

35 40 45

Gly Ala Thr Thr Pro Leu Pro Val Val Leu Gly His Glu Gly Ala Gly

50 55 60

Val Val Gln Lys Val Gly Ala Asn Val Thr Asp Val Lys Pro Gly Asp

65 70 75 80

His Val Val Leu Ser Phe Ser Tyr Cys Gly His Cys Tyr Asn Cys Thr

85 90 95

His Asn His Gln Gly Leu Cys Glu Asn Phe Asn Gln Leu Asn Phe Glu

100 105 110

Gly Lys Thr Tyr Asp Gly Thr His Arg Leu His Leu Asp Asp Gly Thr

115 120 125

Pro Val Ser Val Phe Phe Gly Gln Ser Ser Phe Ala Thr Tyr Val Thr

130 135 140

Ala Asn Val His Asn Ile Val Lys Val Asp Gln Asp Val Asp Leu Asn

145 150 155 160

Leu Leu Gly Pro Leu Gly Cys Gly Met Gln Thr Gly Ala Gly Thr Val

165 170 175

Leu Asn Tyr Ile Lys Pro Ala Pro Glu Asp Ala Ile Ala Val Phe Gly

180 185 190

Ala Gly Ala Val Gly Leu Ala Ala Ile Met Ala Ala Lys Ile Ala Gly

195 200 205

Val Lys His Ile Ile Ala Ile Asn Arg Asn Gly Asn His Leu Asp Leu

210 215 220

Ala Lys Glu Leu Gly Ala Thr Glu Thr Ile Asn Asn Thr Ala Glu Asp

225 230 235 240

Pro Val Lys Ala Ile Lys Glu Ile Val Pro Arg Gly Val Thr Tyr Ala

245 250 255

Ile Asp Thr Thr Gly Asn Thr Gly Val Ile Lys Ser Ala Ile Asp Ser

260 265 270

Leu Ala Thr Ala Gly Glu Cys Val Leu Leu Gly Val Gly Gly Asp Ile

275 280 285

Thr Leu Asp Leu Met Asn Asp Ile Leu Ser Glu Ser Lys Lys Ile Ser

290 295 300

Gly Val Val Glu Gly Asp Ser Asn Pro Gln Glu Phe Ile Pro Gln Leu

305 310 315 320

Val Lys Tyr Tyr Lys Gln Ser Lys Phe Pro Leu Asp Lys Leu Val Lys

325 330 335

Tyr Tyr Asp Phe Ala Asp Ile Asn Gln Val Ile Ala Asp Ser Thr Asn

340 345 350

Gly Lys Val Ile Lys Pro Ile Ile Lys Ile Asp Pro Glu Leu Ala Lys

355 360 365

Leu Pro Leu Thr Asn Asp Gly Ser Asn Val Gln Lys Met Val Ala Glu

370 375 380

Ala Gly Leu Ala Asp Gln Ile Thr Ile Asp Ser Ala Gly Thr Ser Asn

385 390 395 400

Ile Ala Glu Gly Ser Pro Ala Asp Ser Arg Thr Lys Ala Ile Leu Asp

405 410 415

Lys Tyr His Ile Lys Asp Asp Gly Met Ile Ala Arg Gln Leu Gln Asp

420 425 430

Arg Asp Tyr Tyr Asp Ala Asp Tyr Ile Ile Ala Met Asp Gln Met Asn

435 440 445

Val Arg Asp Ala Lys Asp Met Ala Pro Ala Gly Leu Glu Asn Lys Val

450 455 460

His Gly Ile Phe Glu Ala Thr Pro Gly Lys Glu Asn Cys Tyr Ile Val

465 470 475 480

Asp Pro Trp Ile Thr His

485

<210>22

<211>1464

<212>DNA

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>22

atgcaaatta aagctgctct tgcaaccaaa cctaacgctg atttagagat tcaaaccgtc 60

gaattggatg aaccaaaaga aaatgaagta ttaataaaaa ttgcttcaac aggtttttgt 120

catacagata ttgttggtcg aagcggtgcc actacccctc tccccgttgt cctcgggcat 180

gaaggtgcgg gcgtcgtcca aaaagtagga gctaacgtta cggacgttaa acccggcgac 240

catgttgttc tatcatttag ctactgtggc cattgctata actgtactca taatcatcaa 300

ggcttatgcg aaaacttcaa tcagctaaac tttgaaggaa aaacctatga tggtactcac 360

cgcctgcact tagatgatgg cacgccagtc agtgtctttt ttggtcagtc ttcctttgcg 420

acctatgtaa cagccaatgt ccataatatt gttaaagttg atcaagatgt tgatcttaac 480

ttattagggc cactcggttg tggaatgcaa acaggtgctg gaaccgttct aaattatatt 540

aaacctgctc ctgaagatgc aattgccgtt ttcggtgctg gtgctgttgg cttagccgca 600

attatggctg ctaaaattgc tggagttaaa catattattg cgattaatcg taacggtaac 660

caccttgacc tggcgaagga attgggcgct actgaaacga ttaataatac ggctgaagat 720

cccgtcaaag caattaaaga aatcgttccg cgtggtgtaa cttatgcaat cgatactacc 780

ggaaacaccg gtgtaattaa atcagcaatt gatagtcttg ccaccgctgg agaatgtgtc 840

ctcttaggag ttggcggcga tattacctta gacttaatga atgatatctt atcagaatct 900

aagaaaatct ctggggttgt cgaaggagat agcaatcccc aagagtttat tcctcaacta 960

gttaagtact acaagcaaag caagttcccc cttgataagc ttgttaagta ctacgatttt 1020

gctgatatta accaagttat cgctgactca acaaacggaa aggttattaa gccaatcatc 1080

aaaattgatc ctgaattagc taaataattg ccgctcacca atgacggaag caatgttcaa 1140

aaaatggttg cagaagctgg ccttgctgat caaattacta ttgattcagc cggaacaagt 1200

aacattgcag aaggttcacc tgctgatagt cgaacaaaag ccattctcga taaatatcac 1260

attaaagacg acggaatgat tgcccgtcaa ttgcaggaca gggattatta tgatgccgat 1320

tatattatcg caatggatca gatgaatgtc cgggacgcaa aagatatggc accagctggg 1380

ttagaaaata aggttcatgg aatctttgaa gctaccccag gaaaagaaaa ttgctatatc 1440

gttgacccct ggatcactca ctga 1464

<210>23

<211>351

<212>PRT

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>23

Met Lys Lys Ala Ile Phe Glu Lys Ala Gly Gln Met Lys Ile Val Asp

1 5 10 15

Val Asp Arg Pro Thr Ile Glu Lys Pro Asp Asp Val Ile Ile Lys Val

20 25 30

Val Arg Thr Cys Val Cys Gly Ser Asp Leu Trp Asn Phe Arg Gly Ile

35 40 45

Asn Pro Val Glu Lys Asp Ser Glu Asn Ser Gly His Glu Ala Ile Gly

50 55 60

Ile Val Glu Glu Val Gly Glu Asp Ile Thr Thr Val Lys Pro Gly Asp

65 70 75 80

Phe Val Ile Ala Pro Phe Thr His Gly Cys Gly His Cys Ala Ala Cys

85 90 95

Arg Ala Gly Phe Asp Gly Ser Cys Gln Ser His Asn Asp Asn Phe Ser

100 105 110

Ser Gly Val Gln Ala Gln Tyr Val Arg Phe Gln His Gly Gln Trp Ala

115 120 125

Leu Val Lys Val Pro Gly Lys Pro Ser Asp Tyr Ser Glu Gly Met Leu

130 135 140

Lys Ser Leu Leu Thr Leu Ala Asp Val Met Ala Thr Gly Tyr His Ala

145 150 155 160

Ala Arg Val Ala Asn Val Ser Asp Gly Asp Thr Val Val Val Met Gly

165 170 175

Asp Gly Ala Val Gly Leu Cys Ala Ile Ile Ala Ala Lys Met Arg Gly

180 185 190

Ala Lys Lys Ile Ile Ser Thr Ser Arg His Ala Asp Arg Gln Ala Leu

195 200 205

Ala Lys Glu Phe Gly Ala Thr Asp Asn Val Ala Glu Arg Ser Asp Glu

210 215 220

Ala Val Gln Lys Ile Met Glu Leu Thr Asn Gly Ala Gly Ala Asp Ala

225 230 235 240

Val Leu Glu Cys Val Gly Thr Glu Gln Ser Thr Asp Thr Ala Met Lys

245 250 255

Val Gly Arg Pro Gly Thr Ile Val Gly Arg Val Gly Leu Pro His Thr

260 265 270

Pro Lys Met Asp Met Thr Val Leu Phe Tyr Asn Asn Thr Ile Val Gly

275 280 285

Gly Gly Pro Ala Ser Val Thr Thr Tyr Asp Lys Asp Val Leu Leu Lys

290 295 300

Ala Val Leu Asp Gly Asp Ile Asn Pro Gly Lys Val Phe Thr Lys Ser

305 310 315 320

Phe Asp Leu Asp Gln Ile Gln Glu Ala Tyr Glu Ala Met Asp Lys Arg

325 330 335

Glu Ala Ile Lys Ser Tyr Ile Ile Met Asp Gly Phe Glu Arg Asp

340 345 350

<210>24

<211>1056

<212>DNA

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>24

atgaaaaaag ctatttttga aaaggcgggt caaatgaaga ttgttgatgt tgaccgtcca 60

acaattgaaa agcctgatga cgtaattatt aaggtagtgc ggacctgtgt ttgtggttct 120

gacctatgga acttccgagg aattaatccg gttgaaaaag attctgaaaa ctctggccat 180

gaagcaattg gaattgttga agaagttggt gaagatatca ctactgtcaa acctggggac 240

tttgtgattg ctccatttac tcatggatgt gggcactgtg ctgcttgtcg cgcgggcttc 300

gatggttctt gccaaagtca caacgataac tttagctctg gtgtgcaagc tcaatacgtt 360

cggttccaac acggtcaatg ggcgcttgtt aaagttccgg gcaagccaag tgactacagt 420

gaaggaatgc ttaagtccct cttaaccctt gctgatgtta tggctactgg ttaccacgct 480

gcacgagttg ctaacgttag tgatggtgat acagttgttg taatgggtga cggtgctgtt 540

ggcctttgtg cgattattgc tgctaagatg cggggcgcta agaagatcat ttctactagt 600

cgccatgctg accgtcaagc ccttgctaag gaatttggtg ctactgacaa tgttgctgaa 660

cgtagtgacg aagcggttca aaagatcatg gaactcacta acggtgccgg tgctgatgct 720

gtccttgaat gcgttggtac tgaacaatca actgatactg ccatgaaagt tggccgtcca 780

ggtaccatcg ttggtcgggt tggcttacct cataccccaa agatggacat gacggtgcta 840

ttctacaaca acactattgt cggcggtggt ccagcatcag taaccactta cgacaaggac 900

gtattgttga aggctgttct tgatggtgac attaaccctg gtaaggtctt tactaagagc 960

ttcgaccttg accaaattca agaagcttat gaagcaatgg ataagcgtga agcaatcaag 1020

tcttacatta ttatggatgg ctttgaacgc gattaa 1056

<210>25

<211>251

<212>PRT

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>25

Met Gly Arg Leu Asp Asn Lys Val Ala Ile Ile Thr Gly Gly Ser Lys

1 5 10 15

Gly Ile Gly Ala Ala Val Ala Lys Lys Phe Ile Glu Glu Gly Ala Lys

20 25 30

Val Val Leu Thr Ala Arg Lys Met Asp Glu Gly Gln Lys Val Ala Asp

35 40 45

Gln Leu Gly Asp Asn Ala Ile Phe Ile Gln Gln Asp Val Ala Arg Lys

50 55 60

Gly Asp Trp Asp Arg Val Ile Arg Gln Thr Val Gln Val Phe Gly Lys

65 70 75 80

Leu Asn Ile Val Val Asn Asn Ala Gly Ile Ala Glu Tyr Ala Asp Val

85 90 95

Glu Lys Thr Asp Ala Glu Ile Trp Asp Lys Thr Ile Ala Val Asn Leu

100 105 110

Thr Gly Thr Met Trp Gly Thr Lys Leu Gly Ile Glu Ala Met Lys Asn

115 120 125

Asn Gly Glu Lys Asn Ser Ile Ile Asn Met Ser Ser Ile Glu Gly Leu

130 135 140

Ile Gly Asp Pro Asp Leu Phe Ala Tyr Asn Ala Ser Lys Gly Gly Val

145 150 155 160

Arg Leu Leu Thr Lys Ser Ala Ala Leu Asp Cys Ala Arg Lys Gly Tyr

165 170 175

Asp Ile Arg Val Asn Thr Ile His Pro Gly Tyr Ile Ser Thr Pro Leu

180 185 190

Val Asp Asn Leu Val Lys Asp Asp Pro Lys Ala Glu Gly His Leu Glu

195 200 205

Ser Leu His Pro Leu Gly Arg Leu Gly Lys Pro Glu Glu Ile Ala Asn

210 215 220

Leu Ala Leu Tyr Leu Ala Ser Asp Glu Ser Ser Phe Ser Thr Gly Ser

225 230 235 240

Glu Phe Val Ala Asp Gly Gly Tyr Thr Ala Gln

245 250

<210>26

<211>756

<212>DNA

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>26

atgggtcgtt tagataataa agttgcaatt attactggtg gttctaaagg aattggagct 60

gctgtcgcaa aaaagtttat cgaagaaggc gcaaaggttg ttttaaccgc tcggaagatg 120

gatgagggac aaaaagtcgc tgaccaacta ggtgacaatg cgatctttat ccaacaagac 180

gttgctcgga aaggagactg ggaccgggta atccgccaaa ctgtccaagt ctttgggaag 240

ctcaatattg tggttaacaa tgcgggaatt gccgaatacg ccgatgttga gaagacggac 300

gctgaaattt gggataaaac aattgccgtt aaccttaccg gtacgatgtg gggaactaag 360

ctcggtattg aagcaatgaa gaacaacggg gaaaagaatt caatcatcaa tatgtcatcc 420

attgaaggac taattggtga tcctgatctc tttgcataca atgcttctaa gggtggtgtc 480

cgcctcttaa ctaagtccgc tgcgcttgat tgtgcccgga aaggctatga catccgtgta 540

aatacaattc atcctggtta tatctcaact ccactagttg ataatttggt caaggatgat 600

ccaaaagcag aaggacacct agaaagcctt catccccttg gccgtcttgg aaagccagaa 660

gagattgcta acctcgcttt ataccttgct tcagatgaat caagctttag tactggttcg 720

gaatttgtcg ctgatggtgg ctatacggct caataa 756

<210>27

<211>292

<212>PRT

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>27

Met Thr Asn Val Pro Thr Val Lys Leu Asn Asn Gly Val Glu Met Pro

1 5 10 15

Thr Leu Gly Phe Glu Val Phe Gln Val Pro Asp Leu Ser Gln Ala Glu

20 25 30

Gln Ala Val Thr Asp Ala Leu Glu Val Gly Tyr Arg Leu Ile Asp Thr

35 40 45

Ala Ala Ala Tyr Gln Asn Glu Glu Ala Val Gly Lys Ala Ile Lys Asn

50 55 60

Ser Ser Val Asn Arg Glu Asp Val Phe Val Thr Ser Lys Leu Trp Val

65 70 75 80

Ser Asp Phe Asn Tyr Lys Arg Ala Lys Ala Gly Ile Asp Ala Ser Leu

85 90 95

Gln Lys Leu Gly Leu Asp Tyr Met Asp Leu Tyr Leu Leu His Gln Pro

100 105 110

Tyr Gly Asp Thr Met Gly Ala Trp Arg Ala Leu Gln Glu Ala Gln Lys

115 120 125

Glu Gly Lys Ile Arg Ala Ile Gly Val Ser Asn Phe Tyr Ala Asp Gln

130 135 140

Leu Lys Asp Leu Glu Leu Thr Met Pro Val Lys Pro Ala Val Asn Gln

145 150 155 160

Ile Glu Val Asn Pro Trp Tyr Gln Gln Asp Gln Glu Val Lys Phe Ala

165 170 175

Gln Ser Glu Asp Ile Arg Val Glu Ala Trp Ala Pro Phe Ala Glu Gly

180 185 190

Lys His Asp Ile Phe Thr Asn Glu Ile Ile Ala Glu Ile Ala Ala Lys

195 200 205

Tyr Gly Lys Ser Asn Gly Gln Val Ile Leu Arg Trp Leu Leu Gln Arg

210 215 220

Gly Ile Thr Val Ile Pro Lys Ser Val His Lys Asn Arg Met Glu Glu

225 230 235 240

Asn Ile Asp Val Phe Asp Phe Glu Leu Ser Asn Asp Asp Met Lys Lys

245 250 255

Ile Ala Ser Leu Asn Lys Lys Glu Ser Gln Phe Phe Asp His Arg Asp

260 265 270

Pro Val Thr Ile Glu Gln Ile Phe Gly Ser Ser Leu Lys Met Val Gln

275 280 285

Asp Asp Glu Lys

290

<210>28

<211>879

<212>DNA

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>28

atgacaaatg taccaacagt aaaattaaat aacggagtag aaatgccaac ccttggattt 60

gaagtattcc aagttccaga cttaagccaa gctgaacaag cagttaccga tgctcttgaa 120

gtcggctatc gtttaatcga tactgctgct gcttaccaaa atgaagaagc agttggaaag 180

gcaattaaga atagtagtgt aaaccgtgaa gatgtctttg taacttctaa gttatgggtg 240

tctgatttta actataagcg ggctaaagca gggattgacg cttcactgca aaaacttggc 300

cttgattaca tggatcttta ccttctccat caaccatatg gcgatacaat gggggcttgg 360

cgagcattac aagaagcaca gaaagaaggt aagattcgcg caatcggtgt atcgaacttc 420

tacgctgatc aactaaagga tcttgaatta acaatgcctg ttaagccagc ggtcaaccaa 480

attgaagtta acccttggta ccagcaagat caagaggtta agtttgcgca aagtgaagat 540

attcgtgttg aagcatgggc accatttgcg gaaggtaagc atgatatttt taccaacgaa 600

ataattgcgg aaattgctgc caagtatggc aagagcaatg gtcaagtaat tcttcgctgg 660

cttttacaac ggggtattac tgtcattcca aagtcagtcc acaagaaccg gatggaagaa 720

aatatcgatg tctttgattt tgaactttcc aatgatgata tgaaaaagat agctagtctt 780

aacaagaagg aaagccaatt ctttgaccac cgtgatccgg ttacgattga acaaatcttt 840

ggctccagct taaagatggt tcaagatgac gaaaaataa 879

<210>29

<211>287

<212>PRT

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>29

Met Ile Leu Asp Glu Thr Ile Thr Leu Asn Ser Gly Val Lys Ile Pro

1 5 10 15

Lys Phe Ala Leu Gly Thr Trp Met Ile Asp Asp Asp Gln Ala Ala Glu

20 25 30

Ala Val Arg Asn Ala Ile Lys Met Gly Tyr Arg His Ile Asp Thr Ala

35 40 45

Gln Ala Tyr Asp Asn Glu Arg Gly Val Gly Glu Gly Val Arg Thr Ala

50 55 60

Gly Ile Asp Arg Asp Lys Ile Phe Val Thr Ser Lys Ile Ala Ala Glu

65 70 75 80

His Lys Asp Tyr Asp Val Thr Lys Lys Ser Ile Asp Glu Thr Leu Glu

85 90 95

Lys Met Gly Leu Asp Tyr Ile Asp Met Met Leu Ile His Ser Pro Gln

100 105 110

Pro Trp Lys Glu Val Asn Gln Ser Asp Asn Arg Tyr Leu Glu Gly Asn

115 120 125

Leu Ala Ala Trp Arg Ala Met Glu Asp Ala Val Asn Glu Gly Lys Ile

130 135 140

Arg Thr Ile Gly Val Ser Asn Phe Lys Lys Ala Asp Leu Glu Asn Ile

145 150 155 160

Ile Lys Asn Ser Asp Thr Val Pro Ala Val Asp Gln Val Leu Ala His

165 170 175

Ile Gly His Thr Pro Phe Asn Leu Leu Ser Phe Thr His Glu His Asp

180 185 190

Ile Ala Val Glu Ala Tyr Ser Pro Val Ala His Gly Ala Ala Leu Asp

195 200 205

Asn Pro Val Ile Glu Lys Met Ala Lys Lys Tyr Asn Val Ser Val Pro

210 215 220

Gln Leu Cys Ile Arg Tyr Asp Trp Gln Ile Gly Met Ile Val Leu Pro

225 230 235 240

Lys Thr Thr Asn Pro Glu His Met Lys Glu Asn Thr Glu Ile Asp Phe

245 250 255

Glu Ile Ser Glu Ala Asp Met Asp Leu Leu Arg Arg Val Lys Pro Leu

260 265 270

Asp Tyr Gly Asp Phe Asp Ile Tyr Pro Val Tyr Gly Gly Lys Met

275 280 285

<210>30

<211>864

<212>DNA

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>30

atgattttag atgagacaat tactcttaat agtggtgtga aaattccaaa gtttgcatta 60

ggaacctgga tgattgatga tgaccaagca gccgaagcag ttcggaatgc gattaagatg 120

ggatatcggc acatcgatac agctcaggct tatgataatg agcggggagt cggtgaaggt 180

gtacgaacag ccggtattga tcgggataaa atctttgtta cttcaaagat cgctgctgaa 240

cacaaagatt atgatgtaac taaaaagtcg attgacgaga ctcttgaaaa gatgggtctt 300

gattatatcg acatgatgct tattcatagt cctcaaccat ggaaagaagt aaatcaatct 360

gataatcgtt accttgaagg aaatctcgct gcttggcgag ccatggaaga tgccgttaac 420

gaaggtaaga ttcgaacaat tggcgtttct aatttcaaaa aagccgatct tgaaaatatt 480

attaagaata gcgataccgt tcccgctgtt gatcaagttt tagctcatat tggtcatact 540

ccattcaatc ttttatcatt tactcatgaa catgacattg cggttgaagc atattcacca 600

gttgctcacg gcgctgcttt agacaacccc gtaattgaaa agatggctaa aaagtacaac 660

gtttcagtcc cacaattgtg cattcggtat gattggcaaa taggaatgat cgtcttacca 720

aagactacta atccagaaca catgaaggaa aacactgaaa ttgattttga aatttctgaa 780

gctgatatgg acctattgcg gcgagtaaag ccattagact atggcgattt tgatatctac 840

cctgtttacg gtggaaaaat gtaa 864

<210>31

<211>878

<212>PRT

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>31

Met Pro Ala Asn Asn Lys Lys Gln Val Glu Lys Lys Glu Leu Thr Ala

1 5 10 15

Glu Glu Lys Lys Gln Asn Ala Gln Lys Leu Val Asp Asp Leu Met Thr

20 25 30

Lys Ser Gln Ala Ala Phe Glu Lys Leu Arg Tyr Tyr Ser Gln Glu Gln

35 40 45

Val Asp Lys Ile Cys Gln Ala Met Ala Leu Ala Ala Glu Glu His His

50 55 60

Met Asp Leu Ala Val Asp Ala Ala Asn Glu Thr Gly Arg Gly Val Ala

65 70 75 80

Glu Asp Lys Ala Ile Lys Asn Ile Tyr Ala Ser Glu Tyr Ile Trp Asn

85 90 95

Asn Ile Arg His Asp Lys Thr Val Gly Ile Ile Glu Asp Asn Asp Glu

100 105 110

Asp Gln Thr Ile Lys Ile Ala Asp Pro Leu Gly Val Ile Ala Gly Ile

115 120 125

Val Pro Val Thr Asn Pro Thr Ser Thr Thr Ile Phe Lys Ser Ile Ile

130 135 140

Ser Ala Lys Thr Arg Asn Thr Ile Ile Phe Ser Phe His Arg Gln Ala

145 150 155 160

Met Lys Ser Ser Ile Lys Thr Ala Lys Ile Leu Gln Glu Ala Ala Glu

165 170 175

Lys Ala Gly Ala Pro Lys Asn Met Ile Gln Trp Leu Pro Glu Ser Thr

180 185 190

Arg Glu Asn Thr Thr Ala Leu Leu Gln His Pro Asn Thr Ala Thr Ile

195 200 205

Leu Ala Thr Gly Gly Pro Ser Leu Val Lys Ala Ala Tyr Ser Ser Gly

210 215 220

Asn Pro Ala Leu Gly Val Gly Pro Gly Asn Gly Pro Ala Tyr Ile Glu

225 230 235 240

Lys Thr Ala Asn Ile Glu Arg Ser Val Tyr Asp Ile Val Leu Ser Lys

245 250 255

Thr Phe Asp Asn Gly Met Ile Cys Ala Thr Glu Asn Ser Val Val Val

260 265 270

Asp Glu Glu Ile Tyr Asp Lys Val Lys Glu Glu Phe Gln Lys Trp Asn

275 280 285

Cys Tyr Phe Leu Lys Pro Asn Glu Ile Asp Lys Phe Thr Asp Gly Phe

290 295 300

Ile Asp Pro Asp Arg His Gln Val Arg Gly Pro Ile Ala Gly Arg Ser

305 310 315 320

Ala Asn Ala Ile Ala Asp Met Cys Gly Ile Lys Val Pro Asp Asn Thr

325 330 335

Lys Val Ile Ile Ala Glu Tyr Glu Gly Val Gly Asp Lys Tyr Pro Leu

340 345 350

Ser Ala Glu Lys Leu Ser Pro Val Leu Thr Met Tyr Lys Ala Thr Ser

355 360 365

His Glu Asn Ala Phe AspIle Cys Ala Gln Leu Leu Hi s Tyr Gly Gly

370 375 380

Glu Gly His Thr Ala Ala Ile His Thr Leu Asp Asp Asp Leu Ala Thr

385 390 395 400

Lys Tyr Gly Leu Glu Met Arg Ala Ser Arg Ile Ile Val Asn Ser Pro

405 410 415

Ser Gly Ile Gly Gly Ile Gly Asn Ile Tyr Asn Asn Met Thr Pro Ser

420 425 430

Leu Thr Leu Gly Thr Gly Ser Tyr Gly Ser Asn Ser Ile Ser His Asn

435 440 445

Val Thr Asp Trp Asp Leu Leu Asn Ile Lys Thr Ile Ala Lys Arg Arg

450 455 460

Glu Asn Arg Gln Trp Val Lys Ile Pro Pro Lys Val Tyr Phe Gln Arg

465 470 475 480

Asn Ser Leu Lys Glu Leu Gln Asp Ile Pro Asn Ile Asn Arg Ala Phe

485 490 495

Ile Val Thr Gly Pro Gly Met Ser Lys Arg Gly Tyr Val Gln Arg Val

500 505 510

Ile Asp Gln Leu Arg Gln Arg Gln Asn Asn Thr Ala Phe Leu Val Phe

515 520 525

Asp Asp Val Glu Glu Asp Pro Ser Thr Asn Thr Val Glu Lys Gly Val

530 535 540

Ala Met Met Asn Asp Phe Lys Pro Asp Thr Ile Ile Ala Leu Gly Gly

545 550 555 560

Gly Ser Pro Met Asp Ala Ala Lys Ala Met Trp Met Phe Tyr Glu His

565 570 575

Pro Glu Thr Ser Trp Tyr Gly Val Met Gln Lys Tyr Leu Asp Ile Arg

580 585 590

Lys Arg Ala Tyr Gln Ile Lys Lys Pro Thr Lys Ser Gln Leu Ile Gly

595 600 605

Ile Pro Thr Thr Ser Gly Thr Gly Ser Glu Val Thr Pro Phe Ala Val

610 615 620

Ile Thr Asp Ser Lys Thr His Val Lys Tyr Pro Leu Ala Asp Tyr Ala

625 630 635 640

Leu Thr Pro Asn Ile Ala Ile Val Asp Ser Gln Phe Val Glu Thr Val

645 650 655

Pro Ala Lys Thr Thr Ala Trp Thr Gly Leu Asp Val Leu Cys His Ala

660 665 670

Thr Glu Ser Tyr Val Ser Val Met Ala Thr Asp Tyr Thr Arg Gly Trp

675 680 685

Ser Leu Gln Thr Ile Lys Gly Val Met Glu Asn Leu Pro Lys Ser Val

690 695 700

Gln Gly Asp Lys Leu Ala Arg Arg Lys Met His Asp Phe Ser Thr Met

705 710 715 720

Ala Gly Met Ala Phe Gly Gln Ala Phe Leu Gly Ile Asn His Ser Leu

725 730 735

Ala His Lys Met Gly Gly Ala Phe Gly Leu Pro His Gly Leu Leu Ile

740 745 750

Ala Ile Ala Met Pro Gln Val Ile Arg Phe Asn Ala Lys Arg Pro Gln

755 760 765

Lys Leu Ala Leu Trp Pro His Tyr Glu Thr Tyr His Ala Thr Lys Asp

770 775 780

Tyr Ala Asp Ile Ala Arg Phe Ile Gly Leu Lys Gly Asn Thr Asp Glu

785 790 795 800

Glu Leu Ala Glu Ala Tyr Ala Lys Lys Val Ile Glu Leu Ala His Glu

805 810 815

Cys Gly Val Lys Leu Ser Leu Lys Asp Asn Gly Val Thr Arg Glu Glu

820 825 830

Phe Asp Lys Ala Val Asp Asp Leu Ala Arg Leu Ala Tyr Glu Asp Gln

835 840 845

Cys Thr Thr Thr Asn Pro Val Glu Pro Leu Val Ser Gln Leu Lys Glu

850 855 860

Leu Leu Glu Arg Cys Tyr Asp Gly Thr Gly Val Glu Glu Lys

865 870 875

<210>32

<211>2637

<212>DNA

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>32

atgcctgcta acaacaagaa acaagttgaa aagaaagaat taactgctga agaaaaaaag 60

caaaacgccc aaaagctagt tgacgattta atgactaaga gtcaagctgc ttttgaaaag 120

ttacgttact attcacaaga acaagttgac aagatttgtc aggcaatggc tctcgctgcc 180

gaagaacacc acatggactt agctgttgat gctgctaacg aaactggtcg tggggttgct 240

gaagataagg ctatcaagaa catctacgca agtgaataca tttggaacaa catccgtcac 300

gataagactg ttggtattat cgaagacaat gatgaagacc aaactatcaa aattgctgat 360

ccacttggtg tcattgccgg aattgttcca gttactaacc ctacttcaac aacgatcttc 420

aaatcaatca ttagtgctaa gacacggaat acaatcatct tttctttcca ccgtcaagca 480

atgaagtcat ctatcaagac tgcaaagatt ctccaagaag ctgctgaaaa agccggtgcg 540

ccaaagaaca tgattcaatg gctccctgaa agtacccgcg aaaacactac cgcattactc 600

caacacccta atactgctac tattttagca accggtggtc cttcattagt taaggctgcc 660

tacagttctg gtaaccctgc tcttggtgtt ggtcctggta acggtcctgc ttacatcgaa 720

aaaactgcca acatcgaacg ttctgtttac gacatcgttc tttctaagac attcgataac 780

ggtatgattt gtgccactga aaactcagtt gttgttgatg aagaaatcta cgacaaggtt 840

aaagaagaat tccaaaagtg gaactgttac ttcttgaagc caaacgaaat tgataaattt 900

actgatggct ttattgaccc agatcgtcat caagttcgtg gtccaatcgc tggtcgttca 960

gctaatgcta ttgctgacat gtgtggtatt aaagtacctg acaacactaa ggttatcatt 1020

gctgaatacg aaggtgttgg tgacaagtac ccactttcag ctgaaaagct ttcaccagta 1080

ttaacaatgt acaaggcaac ctctcacgaa aatgcctttg atatctgtgc tcaattatta 1140

cactacggtg gtgaaggtca cactgctgct attcacaccc ttgatgatga tttagctact 1200

aagtacggtc ttgaaatgcg tgcttcacgg atcattgtta actccccatc tggtattggt 1260

ggtattggta acatctacaa caacatgact ccatccctta ctttaggtac tggttcatac 1320

ggtagtaact caatttctca caacgttact gattgggacc tcttaaacat caaaacaatt 1380

gcaaagcggc gtgaaaaccg tcaatgggtt aagattcccc caaaagtata ctttcaacgc 1440

aactcactaa aagaattgca agatattcca aacattaacc gggcattcat cgttactggt 1500

cctggaatga gcaagcgtgg ttacgttcaa cgtgttatcg atcaattgcg tcaacgccaa 1560

aacaacactg ctttcttagt atttgatgac gttgaagaag atccatcaac aaacactgtt 1620

gaaaaaggtg ttgccatgat gaatgacttc aaacctgata caattattgc tcttggtggt 1680

ggttcaccaa tggatgctgc taaggctatg tggatgttct atgagcaccc agaaacttca 1740

tggtatgggg ttatgcaaaa gtaccttgat attcggaagc gtgcttacca aatcaagaag 1800

cctactaagt ctcaacttat tggtatccct actacatcag gtactggttc agaagttact 1860

ccatttgcgg ttattaccga ttcaaaaact catgttaagt acccacttgc tgactacgcc 1920

ttaacaccaa acattgcaat cgttgactca caattcgttg aaactgttcc agcaaaaact 1980

actgcttgga ctggactaga tgttttatgt cacgctactg aatcatatgt ttctgttatg 2040

gcaactgact acactcgtgg ttggtcacta caaaccatca agggtgttat ggaaaacctt 2100

cctaagtcag ttcaaggtga taagttagct cgtcgtaaga tgcacgactt ctcaacaatg 2160

gccgggatgg catttggtca agccttctta ggaattaacc actcccttgc ccacaagatg 2220

ggtggagcat tcggtcttcc tcacggtttg cttatcgcta ttgcaatgcc acaagtaatt 2280

cgctttaacg caaaacgtcc acaaaagctt gctctctggc ctcactatga gacttaccat 2340

gcaactaagg actacgctga cattgcacgg ttcattggtt tgaaaggcaa cactgatgaa 2400

gaattagctg aagcatatgc taagaaagtt atcgaacttg ctcacgaatg tggtgttaag 2460

cttagtctta aggacaatgg tgttacacgt gaagaatttg ataaggcggt tgacgatctt 2520

gctcgcttag cttacgaaga tcaatgtact actactaacc cagttgaacc acttgttagc 2580

caactcaagg aattacttga acgttgctac gatggtactg gcgttgaaga aaaataa 2637

<210>33

<211>457

<212>PRT

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>33

Met Ala Tyr Gln Ser Ile Asn Pro Phe Thr Asn Gln Val Glu Lys Thr

1 5 10 15

Phe Glu Asn Thr Thr Asp Glu Glu Leu Glu Gln Thr Leu Thr Thr Ala

20 25 30

His Gln Leu Tyr Leu Asp Trp Arg Lys Tyr Asn Asp Leu Glu Glu Arg

35 40 45

Lys Arg Gln Ile Leu Lys Leu Gly Gln Ile Leu Arg Glu Arg Arg Val

50 55 60

Glu Tyr Ala Thr Val Met Ser Lys Glu Met Gly Lys Leu Ile Ser Glu

65 70 75 80

Ala Glu Gly Glu Val Asp Leu Cys Ala Ser Phe Cys Asp Tyr Tyr Ala

85 90 95

Ala His Ala Asp Glu Phe Leu Gln Pro Lys Ile Ile Ala Thr Thr Ser

100 105 110

Gly Arg Ala Lys Val Leu Lys Gln Ser Leu Gly Ile Leu Val Ala Val

115 120 125

Glu Pro Trp Asn Phe Pro Phe Tyr Gln Ile Ala Arg Val Phe Ile Pro

130 135 140

Asn Phe Ile Ala Gly Asn Pro Met Ile Leu Lys Asp Ala Ser Asn Cys

145 150 155 160

Pro Ala Ser Ala Gln Ala Phe Asn Asp Ala Val Lys Glu Ala Gly Ala

165 170 175

Pro Ala Gly Ser Leu Thr Asn Leu Phe Leu Ser Tyr Asp Gln Val Asn

180 185 190

Lys Ala Ile Ala Asp Lys Arg Val Ala Gly Val Cys Leu Thr Gly Ser

195 200 205

Glu Arg Gly Gly Ala Thr Val Ala Lys Glu Ala Gly Ala Asn Leu Lys

210 215 220

Lys Ser Thr Leu Glu Leu Gly Gly Asn Asp Ala Phe Ile Ile Leu Asp

225 230 235 240

Asp Ala Asp Trp Asp Leu Val Glu Lys Val Ala Pro Ala Ala Arg Leu

245 250 255

Tyr Asn Ala Gly Gln Val Cys Thr Ser Ser Lys Arg Phe Ile Val Leu

260 265 270

Glu Lys Asp Tyr Asp Arg Phe Leu Lys Met Met Lys Asp Ala Phe Ser

275 280 285

Lys Val Lys Met Gly Asp Pro Leu Asp Pro Leu Thr Thr Leu Ala Pro

290 295 300

Leu Ser Ser Lys Lys Ala Lys Glu Lys Leu Gln Gln Gln Val Ala Thr

305 310 315 320

Ala Val Glu Asn Gly Ala Lys Val Tyr Tyr Gly Asn Lys Pro Val Asp

325 330 335

Met Glu Gly Gln Phe Phe Met Pro Thr Ile Leu Thr Asp Ile Thr Pro

340 345 350

Asp Asn Pro Ile Phe Asp Thr Glu Met Phe Gly Pro Val Ala Ser Val

355 360 365

Tyr Lys Val Ser Ser Glu Glu Glu Ala Ile Glu Leu Ala Asn Asn Ser

370 375 380

Ser Tyr Gly Leu Gly Asn Thr Ile Phe Ser Asn Asp Ser Glu His Ala

385 390 395 400

Glu Arg Val Ala Ala Lys Ile Glu Thr Gly Met Ser Trp Ile Asn Ala

405 410 415

Gly Trp Ala Ser Leu Pro Glu Leu Pro Phe Gly Gly Val Lys Asn Ser

420 425 430

Gly Tyr Gly Arg Glu Leu Ser Ser Tyr Gly Ile Asp Glu Phe Thr Asn

435 440 445

Lys His Leu Ile Tyr Glu Ala Arg Gln

450 455

<210>34

<211>1374

<212>DNA

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>34

atggcatatc aaagtatcaa tccatttacg aaccaagtag aaaaaacgtt tgaaaataca 60

actgatgaag aattagaaca aacattaact acggcgcatc aattatattt agattggcgg 120

aagtataatg accttgaaga acggaaacgg caaattttaa agttaggtca aatattacgt 180

gaacggcgtg ttgaatatgc gacagttatg agtaaggaaa tgggaaaatt aattagcgaa 240

gcagaaggcg aggttgacct ttgtgcttct ttctgtgatt attatgcagc ccatgcagat 300

gaatttctgc aaccaaaaat tattgcgaca acgagtggac gcgccaaagt tttgaagcaa 360

tcattaggaa ttttagttgc agttgaacct tggaatttcc cattctatca aattgcccgg 420

gtatttattc ccaactttat tgcaggaaac cccatgatct tgaaggatgc gtcgaattgt 480

ccagcatccg cccaagcatt taacgatgcc gttaaggaag ctggtgcgcc agccggcagt 540

ttaactaatt tattcctttc atatgaccaa gtaaataagg caattgctga taagcgggta 600

gccggcgttt gtcttactgg ttctgaacgt ggtggtgcaa ccgttgctaa agaggctggt 660

gctaatttga agaagagcac tttggaactt ggtggtaatg atgcctttat tatcttagac 720

gatgcagatt gggatcttgt cgaaaaagtt gccccggcag cccgtctgta taatgctgga 780

caagtatgta catcatcaaa acgttttatt gtccttgaaa aggattatga tcgtttctta 840

aagatgatga aagatgcgtt ctcgaaagtt aaaatgggtg atccccttga tccattaaca 900

actctggcac cattatcatc taagaaagca aaagaaaagc tccaacagca agtcgcaaca 960

gcagtagaaa atggggccaa agtttactat ggtaataagc cggttgacat ggaaggtcaa 1020

ttctttatgc caacgatctt aactgatatc actccagata acccaatatt tgatacggaa 1080

atgtttgggc cagtggcttc ggtttataag gttagttccg aagaggaagc aatcgaactg 1140

gctaataatt caagctatgg gttaggaaac actatcttta gcaatgattc cgaacatgcg 1200

gaacgagtag cagcgaagat cgaaactgga atgagttgga ttaatgccgg ctgggcttca 1260

ttaccagaat taccatttgg tggtgttaag aattcaggtt acggtcgtga actcagcagt 1320

tacggaattg atgaatttac taacaaacat ctaatttacg aagcacgaca ataa 1374

<210>35

<211>477

<212>PRT

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>35

Met Gln Ile Asn Asp Ile Glu Ser Ala Val Arg Lys Ile Leu Ala Glu

1 5 10 15

Glu Leu Asp Asn Ala Ser Ser Ser Ser Ala Asn Val Ala Ala Thr Thr

20 25 30

Asp Asn Gly His Arg Gly Ile Phe Thr Asn Val Asn Asp Ala Ile Ala

35 40 45

Ala Ala Lys Ala Ala Gln Glu Ile Tyr Arg Asp Lys Pro Ile Ala Val

50 55 60

Arg Gln Gln Val Ile Asp Ala Ile Lys Glu Gly Phe Arg Pro Tyr Ile

65 70 75 80

Glu Lys Met Ala Lys Asp Ile Lys Glu Glu Thr Gly Met Gly Thr Val

85 90 95

Glu Ala Lys Ile Ala Lys Leu Asn Asn Ala Leu Tyr Asn Thr Pro Gly

100 105 110

Pro Glu Ile Leu Glu Pro Val Val Glu Asn Gly Asp Gly Gly Met Val

115 120 125

Met Tyr Glu Arg Leu Pro Tyr Gly Val Ile Gly Ala Val Gly Pro Ser

130 135 140

Thr Asn Pro Ser Glu Thr Val Ile Ala Asn Ala Ile Met Met Leu Ala

145 150 155 160

Gly Gly Asn Thr Leu Tyr Phe Gly Ala His Pro Gly Ala Lys Asn Val

165 170 175

Thr Arg Trp Thr Ile Glu Lys Met Asn Asp Phe Ile Ala Asp Ala Thr

180 185 190

Gly Leu His Asn Leu Val Val Ser Ile Glu Thr Pro Thr Ile Glu Ser

195 200 205

Val Gln Gln Met Met Lys His Pro Asp Ile Ala Met Leu Ala Val Thr

210 215 220

Gly Gly Pro Ala Val Val His Gln Ala Met Thr Ser Gly Lys Lys Ala

225 230 235 240

Val Gly Ala Gly Pro Gly Asn Pro Pro Ala Met Val Asp Ala Thr Ala

245 250 255

Asp Ile Asp Leu Ala Ala His Asn Ile Ile Thr Ser Ala Ser Phe Asp

260 265 270

Asn Asp Ile Leu Cys Thr Ala Glu Lys Glu Val Val Ala Glu Ser Ser

275 280 285

Ile Lys Asp Glu Leu Ile Arg Lys Met Gln Asp Glu Gly Ala Phe Val

290 295 300

Val Asn Arg Glu Gln Ala Asp Lys Leu Ala Asp Met Cys Ile Gln Glu

305 310 315 320

Asn Gly Ala Pro Asp Arg Lys Phe Val Gly Lys Asp Ala Thr Tyr Ile

325 330 335

Leu Asp Gln Ala Asn Ile Pro Tyr Thr Gly His Pro Val Glu Ile Ile

340 345 350

Cys Glu Leu Pro Lys Glu His Pro Leu Val Met Thr Glu Met Leu Met

355 360 365

Pro Ile Leu Pro Val Val Ser Cys Pro Thr Phe Asp Asp Val Leu Lys

370 375 380

Thr Ala Val Glu Val Glu Lys Gly Asn His His Thr Ala Thr Ile His

385 390 395 400

Ser Asn Asn Leu Lys His Ile Asn Asn Ala Ala His Arg Met Gln Cys

405 410 415

Ser Ile Phe Val Val Asn Gly Pro Ser Tyr Val Gly Thr Gly Val Ala

420 425 430

Asp Asn Gly Ala His Ser Gly Ala Ser Ala Leu Thr Ile Ala Thr Pro

435 440 445

Thr Gly Glu Gly Thr Cys Thr Ala Arg Thr Phe Thr Arg Arg Val Arg

450 455 460

Leu Asn Ser Pro Gln Gly Phe Ser Val Arg Asn Trp Tyr

465 470 475

<210>36

<211>1434

<212>DNA

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>36

atgcagatta atgatattga aagtgctgta cgcaaaattc ttgccgaaga actagataat 60

gccagctctt caagtgcaaa cgttgcagct actactgata atggtcatcg cggaattttc 120

actaatgtca atgatgcaat tgctgctgca aaagctgctc aagaaatata tcgggataag 180

ccaattgctg ttcgccaaca agtgattgat gccattaagg aaggattccg cccatatatt 240

gaaaaaatgg ctaaagatat caaagaagaa acaggaatgg gaacagtaga ggccaaaatt 300

gctaagttaa acaatgcctt gtacaacact cctggtcccg agattcttga accagttgta 360

gaaaacggtg acggtgggat ggttatgtat gaacggttac catatggtgt tattggtgcg 420

gttggcccaa gtacaaaccc ttcagaaact gtaattgcta atgcgatcat gatgcttgcc 480

ggtggtaata ctctttactt tggtgctcac cctggcgcaa agaatgttac tcgctggaca 540

attgaaaaga tgaacgattt tattgcagat gcaacaggcc ttcataattt agttgtaagt 600

attgaaacac caacaattga atcagttcaa caaatgatga agcaccccga cattgcaatg 660

ttagcagtaa ctggtggccc agctgttgtt caccaagcaa tgaccagtgg taagaaagcg 720

gttggtgctg gtcctggtaa tcctcctgca atggttgatg ctactgctga tattgattta 780

gctgctcata atatcattac atctgcttca tttgataatg atattttatg tactgctgaa 840

aaggaagtag ttgcagaaag tagcattaaa gatgaattaa ttcgtaagat gcaagatgaa 900

ggtgcctttg tagttaaccg tgaacaagcc gataaattag ctgatatgtg tatccaagaa 960

aatggtgctc ctgatcgtaa atttgttggt aaggatgcaa cttatatctt agaccaagct 1020

aatattcctt acacaggcca cccagttgaa attatttgtg aacttcctaa ggaacatcca 1080

ttagtaatga ctgaaatgtt aatgccaatt ttaccagttg tttcttgtcc aacatttgat 1140

gatgttttga agactgctgt tgaagttgaa aaaggtaacc atcacacagc tactattcat 1200

tccaataacc ttaagcatat taataatgct gctcaccgga tgcaatgttc aatctttgtt 1260

gttaatggcc catcctatgt tggtacaggt gttgcagata atggagctca ctcaggtgct 1320

tcagcattaa caattgctac gccaactggt gaaggaacat gtactgcacg aacatttact 1380

cgtcgggttc gtttgaactc accacaagga ttctcagtac gtaactggta ttaa 1434

<210>37

<211>383

<212>PRT

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>37

Met Lys Phe Val Ile Ala Pro Asp Ser Phe Lys Gly Gly Leu Thr Ala

1 5 10 15

Lys Glu Ala Ala Asn Val Met Ala Glu Gly Ile Lys Arg Val Phe Pro

20 25 30

Asn Ala Glu Tyr Ala Leu Val Pro Met Ala Asp Gly Gly Glu Gly Thr

35 40 45

Val Gln Ser Leu Val Asp Ala Thr Asn Gly Gln Lys Met Ile Ala Lys

50 55 60

Val His Asn Pro Leu Asn Lys Leu Val Asn Ala Glu Tyr Gly Ile Leu

65 70 75 80

Gly Asp Gly Glu Thr Ala Val Ile Glu Met Ala Ala Ala Ser Gly Leu

85 90 95

Gln Phe Val Asn Lys Glu Thr Ala Asn Pro Leu Ile Thr Thr Thr Tyr

100 105 110

Gly Thr Gly Glu Leu Ile Lys Asp Ala Leu Asp His Asn Ile Lys Lys

115 120 125

Ile Ile Ile Gly Ile Gly Gly Ser Ala Thr Val Asp Gly Gly Ala Gly

130 135 140

Met Ala Gln Ala Leu Gly Ala Arg Leu Leu Asp Ala Asp Asn His Glu

145 150 155 160

Ile Gly Leu Gly Gly Gly Glu Leu Ala Ser Leu Glu Gln Val Asp Phe

165 170 175

Gly Gly Leu Asp Pro Arg Leu Lys Asn Val Asp Ile Gln Ile Ala Ser

180 185 190

Asp Val Thr Asn Pro Leu Thr Gly Lys Asn Gly Ala Ala Pro Val Phe

195 200 205

Gly Pro Gln Lys Gly Ala Asp Glu Glu Met Val Asn Ile Leu Asp Lys

210 215 220

Asn Leu His His Tyr Ala Arg Lys Ile Val Ala Ala Gly Gly Pro Asp

225 230 235 240

Val Glu Gln Thr Ala Gly Ala Gly Ala Ala Gly Gly Leu Gly Ala Gly

245 250 255

Leu Ile Ala Phe Thr Gly Ala Thr Met Lys Arg Gly Val Glu Leu Val

260 265 270

Ile Glu Ala Thr Gln Leu Gln Lys Lys Ala Val Gly Ala Asp Tyr Val

275 280 285

Phe Thr Gly Glu Gly Gly Ile Asp Phe Gln Thr Lys Phe Gly Lys Thr

290 295 300

Pro Tyr Gly Val Ala Lys Ala Thr Lys Glu Val Ala Pro Thr Ala Pro

305 310 315 320

Val Ile Val Leu Ala Gly Asn Ile Gly Lys Gly Val Asn Asp Leu Tyr

325 330 335

Ser Ser Thr Ala Ile Asp Ala Ile Phe Ala Thr Pro Glu Gly Ala Lys

340 345 350

Pro Leu Lys Thr Ala Leu Ala Asp Ala Pro Ile Asp Ile Ala Gln Thr

355 360 365

Ala Glu Asn Val Ala Arg Leu Ile Lys Val Ser His Val Ser Asn

370 375 380

<210>38

<211>1152

<212>DNA

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>38

atgaaatttg taattgctcc agattcattt aaaggcggat taacagcaaa agaagcagca 60

aatgtgatgg cagaaggaat caaaagagtg tttccgaatg ccgagtatgc tttagttcca 120

atggctgatg gaggagaggg gactgttcaa tccttagttg atgcgactaa cggtcaaaaa 180

atgattgcta aagtccacaa cccattaaat aaattagtta atgctgagta cggaatatta 240

ggtgatgggg aaacggcagt gattgagatg gcggcggcaa gtggccttca atttgttaat 300

aaggagactg cgaacccgct tattacaact acatatggta ccggcgagtt aattaaggat 360

gctcttgacc ataacattaa aaaaataatt attggaattg gtggaagtgc aaccgttgat 420

ggcggagcgg ggatggccca agcacttgga gcacgtttat tggatgctga taatcatgaa 480

attggtttag gcggtggtga gttagcaagt ttagagcaag tagattttgg aggattagat 540

cctcgcttaa aaaatgtaga tattcagatt gcatcagacg taaccaaccc attaacagga 600

aaaaatgggg cagccccagt atttggcccg caaaaaggag ctgatgaaga aatggtgaac 660

atcttggaca aaaatcttca tcattatgcc cgaaaaatag ttgcagctgg tgggccagac 720

gttgaacaaa cggcaggtgc aggggcagcc ggtggtttag gagccgggtt gatagcattt 780

accggtgcga caatgaagcg aggagtagaa ttagtgattg aagcaactca actacaaaaa 840

aaggcagttg gcgctgatta tgtttttact ggtgaaggag gaattgattt ccagactaaa 900

tttggtaaaa cgccatatgg agtcgctaag gcaactaaag aggtggctcc aactgctccg 960

gtaattgtgt tggctggaaa tattggtaaa ggcgtaaatg atctatattc atccacggcc 1020

attgatgcaa tttttgcaac tcctgaaggg gctaaaccat taaaaacagc attagcagat 1080

gcacctattg atattgctca aacagcggaa aacgttgcac gtttaattaa agtgagtcat 1140

gttagtaatt aa 1152

<210>39

<211>500

<212>PRT

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>39

Leu Ser Glu Gln Gln Tyr Ile Met Ala Ile Asp Gln Gly Thr Thr Ser

1 5 10 15

Ser Arg Ala Ile Ile Phe Asp His Asp Gly Asn Lys Val Ala Ile Ser

20 25 30

Gln Gln Glu Phe Pro Gln Tyr Phe Pro Gln Pro Gly Trp Val Glu His

35 40 45

Asp Pro Leu Glu Ile Trp Asp Ser Val Gln Ser Val Ile Ser Asn Val

50 55 60

Met Ile Lys Ser Gln Ile Lys Pro Tyr Lys Ile Ala Ala Ile Gly Ile

65 70 75 80

Thr Asn Gln Arg Glu Thr Thr Val Ile Trp Asp Arg His Thr Gly Lys

85 90 95

Pro Ile Tyr Asn Ala Ile Val Trp Gln Ser Lys Gln Thr Ser Asp Ile

100 105 110

Ala Glu Gln Leu Ile Lys Asp Gly Tyr Lys Asp Met Ile His Gln Lys

115 120 125

Thr Gly Leu Val Ile Asp Ser Tyr Phe Ala Ala Thr Lys Ile Lys Trp

130 135 140

Ile Leu Asp His Val Pro Gly Ala Arg Glu Lys Ala Ala Lys Gly Asp

145 150 155 160

Leu Met Phe Gly Thr Ile Asp Thr Trp Leu Leu Trp Asn Leu Ser Gly

165 170 175

Arg Arg Val His Ala Thr Asp Val Thr Asn Ala Ser Arg Thr Met Leu

180 185 190

Phe Asn Ile His Thr Leu Asp Trp Asp Gln Asp Ile Leu Asp Leu Leu

195 200 205

Asp Ile Pro Gln Ser Leu Leu Pro Val Val Lys Pro Ser Ser Ala Ile

210 215 220

Tyr Gly Tyr Thr Gly Asp Tyr His Phe Tyr Gly Val Gln Ile Pro Ile

225 230 235 240

Ala Gly Ile Ala Gly Asp Gln Gln Ala Ala Leu Phe Gly Gln Ala Ala

245 250 255

Tyr Asp Lys Gly Ser Ile Lys Asn Thr Tyr Gly Thr Gly Ala Phe Ile

260 265 270

Val Met Asn Thr Gly Leu Lys Pro Thr Leu Ser Asp Asn Gly Leu Leu

275 280 285

Thr Thr Ile Ala Tyr Gly Leu Asp Gly Gln Thr His Tyr Ala Leu Glu

290 295 300

Gly Ser Ile Phe Val Ala Gly Ser Ala Val Gln Trp Leu Arg Asp Gly

305 310 315 320

Leu Lys Met Phe Asp Lys Ala Ser Glu Ser Glu Gln Met Ala Val Asp

325 330 335

Ala Lys Thr Thr Gly Gly Val Tyr Val Val Pro Ala Phe Thr Gly Leu

340 345 350

Gly Ala Pro Tyr Trp Asp Gln Glu Val Arg Gly Ala Met Phe Gly Leu

355 360 365

Thr Arg Gly Thr Glu Arg Gly His Ile Ile Arg Ala Thr Leu Glu Ala

370 375 380

Ile Ala Tyr Gln Thr Lys Asp Val Val Asp Thr Met Val Lys Asp Thr

385 390 395 400

Gln Leu Pro Leu Thr Ala Leu Thr Val Asn Gly Gly Ala Ser Arg Asn

405 410 415

Asn Phe Met Met Gln Phe Gln Ala Asp Ile Leu Gln Thr Pro Ile Lys

420 425 430

Arg Ala Ala Met Glu Glu Thr Thr Ala Leu Gly Ala Ala Phe Leu Ala

435 440 445

Gly Leu Ala Val Asp Phe Trp Glu Asp Gln Asp Glu Leu Arg Lys Leu

450 455 460

Ser Arg Ile Gly Asp Gln Phe Asp Pro Gln Met Asp Pro Gln Lys Ala

465 470 475 480

Ala Asp Leu Tyr Arg Gly Trp Gln Arg Ala Ile Ala Ala Ala Gln Phe

485 490 495

Tyr Gly Lys Asp

500

<210>40

<211>1503

<212>DNA

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>40

ttgagtgaac aacaatatat catggcgatt gaccagggaa cgacgagctc acgggcgatt 60

atctttgacc atgacggaaa taaggttgcg atcagtcagc aggaatttcc ccaatacttc 120

ccgcagccgg ggtgggttga acatgatcct ctagagattt gggatagcgt tcaatcagtg 180

atttcaaatg taatgattaa gtcccagatc aagccctata agattgcggc aattgggatt 240

actaaccaac gggagacgac ggttatttgg gatcgccata ccggtaagcc gatttataac 300

gcaattgtct ggcaatcgaa gcaaacgagc gacatcgccg aacaattgat taaagatggt 360

tataaggata tgatccacca gaagactggc ttggtgattg attcgtattt cgcggccact 420

aagatcaagt ggatccttga ccatgttcct ggtgcccggg aaaaagcagc aaagggagac 480

ttgatgtttg ggactatcga tacttggtta ctatggaatt tatcgggacg gcgggtccac 540

gcaacggatg tgaccaatgc cagccggacg atgcttttta atatccatac cctcgactgg 600

gatcaagata tccttgacct gcttgatatt ccccagtcgc ttttgccagt agtaaagcca 660

agttcagcca tttacggtta tactggcgac taccacttct atggggtgca gattccaatt 720

gccgggattg caggtgacca acaagcagcc ctctttggtc aagcagccta tgataaaggt 780

tcaatcaaga acacctatgg gactggagcc ttcatcgtca tgaatacggg actaaaaccc 840

acgctttcgg ataacggctt gttgacgacg attgcgtatg gcctggacgg gcaaactcat 900

tacgcgcttg aaggaagtat ctttgtggcc ggttctgccg ttcaatggtt gcgggatggt 960

ctcaagatgt ttgataaggc aagcgagtcc gaacaaatgg ctgtcgatgc caagacaact 1020

ggcggcgttt atgtcgtccc cgcctttaca ggattaggcg caccgtactg ggatcaagaa 1080

gtgcggggcg caatgtttgg ccttacccgt ggaactgaac ggggacatat catccgtgca 1140

actttggaag ccattgccta ccagaccaaa gatgttgtcg atacgatggt caaggacacc 1200

caattaccac taacagcact aacggttaac gggggcgctt cacggaacaa cttcatgatg 1260

cagttccagg ccgatatctt acaaacgcca atcaagcggg cagcaatgga agagacaacc 1320

gcgctgggag cagcctttct cgctggattg gccgttgatt tctgggaaga ccaggatgag 1380

ttacggaagc tatcacggat tggcgaccag tttgatccac aaatggatcc gcaaaaggca 1440

gctgacttgt atcggggatg gcaacgggcc attgcagctg cgcagtttta tggcaaagat 1500

taa 1503

<210>41

<211>338

<212>PRT

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>41

Met Ala Glu Lys Ile Ala Val Leu Gly Ala Gly Ser Trp Gly Ser Val

1 5 10 15

Leu Ala Asn Met Leu Thr Glu Asn Gly His Asp Val Thr Leu Trp Ser

20 25 30

Arg Asn Glu Glu Gln Val Lys Gln Leu Asn Thr Glu His Thr Asn Pro

35 40 45

Arg Tyr Met Lys Asp Phe Val Tyr Ser Thr Asn Leu Thr Ala Thr Thr

50 55 60

Asp Met Lys Lys Ala Val Lys Gly Ala Ser Val Val Leu Ile Val Ile

65 70 75 80

Pro Thr Lys Gly Leu Arg Glu Val Ala Lys Gln Leu Asn Ala Ile Leu

85 90 95

Thr Glu Leu His Gln Lys Pro Leu Val Ile His Ala Thr Lys Gly Leu

100 105 110

Glu Gln Asn Thr Tyr Lys Arg Pro Ser Glu Met Leu Ser Glu Asp Ile

115 120 125

Ser Pro Glu Asn Arg Gln Ala Ile Val Val Leu Ser Gly Pro Ser His

110 135 140

Ala Glu Asp Val Ala Ile Lys Asp Met Thr Ala Val Thr Ala Ala Cys

145 150 155 160

Glu Asp Leu Ala Ser Ala Lys Lys Ala Gln Lys Leu Phe Ser Asn Ser

165 170 175

Tyr Phe Arg Val Tyr Thr Asn Asp Asp Val Ile Gly Ala Glu Phe Gly

180 185 190

Ala Ala Leu Lys Asn Ile Ile Ala Ile Gly Ala Gly Ala Ile Gln Gly

195 200 205

Leu Gly Tyr His Asp Asn Ala Arg Ala Ala Leu Ile Thr Arg Gly Leu

210 215 220

Ala Glu Ile Arg Arg Leu Gly Val Ala Phe Gly Ala Asn Pro Met Thr

225 230 235 240

Phe Ile Gly Leu Ser Gly Val Gly Asp Leu Val Val Thr Ala Thr Ser

245 250 255

Lys Asn Ser Arg Asn Trp Arg Ala Gly Tyr Gln Leu Gly Gln Gly Lys

260 265 270

Lys Leu Gln Asp Val Ile Asp Asn Met Gly Met Val Ile Glu Gly Val

275 280 285

Tyr Thr Thr Lys Ala Ala Tyr Glu Leu Ser Arg Lys Arg Gln Val Gln

290 295 300

Met Pro Ile Thr Glu Ala Leu Tyr Arg Val Leu Tyr Glu Gly Glu Asp

305 310 315 320

Ile Lys Thr Ala Ile Ser Gln Leu Met Asp Arg Asp Leu Thr Ser Glu

325 330 335

Asn Glu

<210>42

<211>1017

<212>DNA

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>42

atggcagaaa aaattgctgt tttaggtgct ggttcgtggg gcagtgtttt agcaaacatg 60

cttacagaaa atggccacga tgtaacatta tggtctcgta atgaggaaca agttaagcaa 120

ttaaatactg aacatacaaa tcctcgctat atgaaagatt ttgtttattc tactaactta 180

acagcaacaa cggacatgaa aaaagctgtt aagggtgcca gtgtggtcct gattgtaatt 240

ccaacaaagg gtcttcgtga agttgctaag caattaaatg caattttgac tgaattacat 300

caaaaaccgc tagttattca cgcaacgaaa ggcttagaac aaaatactta taagcggcca 360

tcggaaatgc ttagcgaaga tatttctcct gaaaaccgtc aggcaattgt tgttttatca 420

ggtccgagtc atgctgaaga tgtggcgatt aaagatatga cagctgtaac cgcagcttgt 480

gaggacctgg ccagtgctaa aaaggcgcag aagttattta gtaattctta tttccgtgtg 540

tacactaatg acgatgtaat tggtgccgaa tttggcgcag ccttaaagaa cattattgca 600

attggtgctg gagctattca gggacttggt tatcatgata atgctcgggc agcgttaatt 660

actcgtggac ttgcagaaat tcgccgattg ggagttgctt ttggtgccaa cccgatgact 720

tttattggtc tttctggggt tggtgacctt gttgttactg ctaccagtaa aaattctcga 780

aattggcgtg ctggctatca attggggcaa ggaaaaaagc ttcaagatgt aattgataat 840

atgggaatgg ttatcgaagg tgtctatact accaaagccg cttatgaatt aagtcgtaaa 900

cgacaagtac agatgccaat taccgaagct ctttaccgtg ttttgtatga aggcgaagat 960

attaaaactg caatttctca attaatggac cgagatctta cttcagaaaa cgaataa 1017

<210>43

<211>256

<212>PRT

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>43

Met Arg Lys Pro Phe Ile Ala Ala Asn Trp Lys Met His Lys Asn Val

1 5 10 15

Gln Glu Ser Val Glu Phe Val Asp Ala Ile Lys Gly Lys Leu Pro Asp

20 25 30

Pro Gln Glu Val Glu Val Gly Ile Ala Ala Gln Ala Phe Ala Leu Pro

35 40 45

Ser Met Val Gln Ala Ala Asp Asp Ser Gly Leu Lys Ile Ile Ala Gln

50 55 60

Asn Ala Ala Ala Glu Tyr Ser Gly Ala Phe Thr Gly Glu Ile Ser Leu

65 70 75 80

Arg Gly Leu Ala Asp Ala Gly Val Ser Tyr Val Met Leu Gly His Ile

85 90 95

Glu Arg Arg His Leu Phe His Glu Asp Asn Glu Leu Val Asn Arg Lys

100 105 110

Val Leu Ala Ala Leu Gln Met Gly Val Thr Pro Ile Ile Cys Thr Asp

115 120 125

Glu Thr Met Val Gln Lys Glu Val Asn Gly Glu Ile His Tyr Val Phe

130 135 140

Gln Gln Leu Met Ser Val Leu Arg Gly Val Ser Leu Asp Gln Ile Lys

145 150 155 160

Asn Val Val Val Ser Tyr Glu Pro Ser Trp Ala Val Gly Tyr Gly Gln

165 170 175

His Ala Asn Pro Val Leu Ala Glu Glu Gly Cys Arg Gln Ile Arg Arg

180 185 190

Thr Ile Ala Asp Asn Tyr Thr Tyr Glu Ile Ala Asp Lys Ile Arg Ile

195 200 205

Leu Tyr Gly Gly Ser Val Asn Pro Asp Asn Ile Gly Met Ile Met Asn

210 215 220

Lys Pro Asp Val Asp Gly Val Leu Ile Gly Arg Ala Ser Leu Asp Val

225 230 235 240

Asp Asn Phe Leu Arg Met Val Asn Tyr Leu Lys Asn Asp Gln Glu Lys

245 250 255

<210>44

<211>771

<212>DNA

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>44

atgcgcaaac cctttattgc tgctaattgg aagatgcata agaatgtcca agaatcggtt 60

gaatttgtgg atgcaattaa aggaaagcta ccagatccgc aagaagttga agtcggaatt 120

gcagcccaag cttttgcatt acccagtatg gttcaagccg ctgatgattc aggattaaag 180

ataatcgcgc aaaacgcggc ggctgaatat tcgggagctt tcactggtga aattagctta 240

cgaggtttag ctgacgccgg tgtttcatat gtaatgttag gacatattga acggcgccat 300

ttattccacg aggataatga gttggttaat cggaaagtgt tggcagccct tcaaatggga 360

gttaccccga taatttgtac ggatgaaacg atggtccaga aagaagttaa tggtgaaatt 420

cactacgttt tccagcaatt gatgagcgta ttgaggggcg tttctcttga tcaaattaaa 480

aatgtagttg tttcctatga accaagttgg gcagttggat atggtcagca tgctaatcca 540

gttcttgctg aagaaggatg ccgtcaaatt cggcgaacga ttgctgataa ctacacttat 600

gagattgctg ataagatcag gattctttat gggggcagtg tcaatccaga taatatcgga 660

atgattatga acaagccaga tgtagatggg gtattaatcg gtcgggcaag tttagatgtt 720

gataattttt tgcgaatggt caattattta aaaaatgatc aagaaaaata a 771

<210>45

<211>259

<212>PRT

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>45

Met Arg Lys Pro Phe Ile Ile Ala Asn Trp Lys Met Asn Lys Asn Val

1 5 10 15

His Glu Ser Val Ala Phe Val Lys Ala Ile Lys Glu Lys Leu Pro Ala

20 25 30

Asp Lys Glu Ile Gly Ile Ala Ala Gln Ala Val Ser Leu Tyr Asn Met

35 40 45

Lys Lys Val Ala Ser Ser Ser Asn Leu Gln Ile Ile Ala Gln Asn Ala

50 55 60

Ser Ala Glu Leu Glu Gly Pro Tyr Thr Gly Glu Ile Ser Met Arg Ser

65 70 75 80

Leu Ala Asp Ala Gly Val Thr Tyr Val Met Leu Gly His Leu Glu Arg

85 90 95

Arg Arg Leu Phe Asn Glu Ser Asn Asp Ser Ile Asn Gln Lys Val Leu

100 105 110

Ala Ala Leu Asn Ala Gly Ile Ile Pro Ile Ile Cys Thr Asp Glu Glu

115 120 125

Met Val Gln Thr Glu Val Asn Gly Gln Ile His Tyr Val Phe Arg Gln

130 135 140

Leu Lys Ser Val Leu Lys Gly Val Pro Ala Asn Lys Leu Ser Gln Ile

145 150 155 160

Val Ile Ser Tyr Glu Pro Ser Trp Ala Val Gly Ser Thr His Gln Ala

165 170 175

Asn Pro Asp Ile Ala Glu Glu Gly Cys Gln Ala Ile Arg Gln Ser Leu

180 185 190

Val Glu Met Tyr Gly Asn Glu Ile Gly Glu Gln Val Arg Ile Leu Tyr

195 200 205

Gly Gly Ser Val Asn Pro Glu Asn Ile Gly Gln Ile Met Ser Lys Pro

210 215 220

Asn Val Asp Gly Ala Leu Ile Gly Arg Ala Ser Leu Glu Ile Glu Ser

225 230 235 240

Phe Leu Gln Met Ile Asn Tyr Ile Glu Leu Ala Ser Lys Gln Lys Leu

245 250 255

Gln Val Ile

<210>46

<211>780

<212>DNA

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>46

atgcgcaaac cgtttattat tgcgaactgg aaaatgaata aaaacgttca tgaatctgtt 60

gcgtttgtta aagcaattaa agaaaagctc ccggcagata aagaaattgg gatcgccgcg 120

caagcagttt cgctatataa catgaaaaaa gtggcgagct cttccaactt acaaattatt 180

gctcaaaatg catctgctga gttagaggga ccatatactg gagaaattag catgcgaagt 240

ttagcagatg cgggcgtgac atacgtgatg ctaggccatt tagagcgccg acgccttttt 300

aacgagagta atgattcaat taatcaaaaa gttttagcag ccctcaatgc tggtattatt 360

ccaatcattt gtacggatga agagatggtc caaacagaag ttaacggaca aattcattat 420

gtatttcgcc aactaaaaag cgtccttaaa ggggtaccag ctaataaact atcacagatt 480

gttatttcgt atgaaccaag ttgggccgtt gggagcacgc atcaagcaaa tccagacatt 540

gcggaagagg gatgtcaggc aattcgtcaa agcctggttg aaatgtatgg taatgagatt 600

ggcgagcaag tccgaatact ctatggtggc agcgttaatc ccgagaacat tggtcaaatt 660

atgagtaaac caaatgttga tggggcgcta atcggtcgcg caagtctcga gattgaaagt 720

ttcttacaaa tgattaatta tatcgaatta gcgagcaagc agaagttaca ggtaatttag 780

<210>47

<211>249

<212>PRT

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>47

Met Arg Val Pro Ile Ile Ala Gly Asn Trp Lys Met His Lys Asp Val

1 5 10 15

Gln Glu Ala Val Ser Phe Ile Glu Lys Val Lys Asn Gln Leu Pro Pro

20 25 30

Ala Asp Gln Leu Glu Thr Ala Ile Ala Ala Pro Thr Leu Cys Leu Val

35 40 45

Pro Met Val Lys Ala Ala Glu Glu Ser Pro Leu Lys Ile Met Ala Glu

50 55 60

Asn Cys Tyr Tyr Lys Asn Glu Gly Ala Tyr Thr Gly Glu Thr Ser Pro

65 70 75 80

Tyr Ala Leu Tyr Gln Ala Gly Ile His His Val Ile Leu Gly His Ser

85 90 95

Glu Arg Arg Thr Tyr Phe Asn Glu Thr Asp Glu Leu Ile Asn Lys Lys

100 105 110

Val Lys Ala Ala Leu Val Asn Gly Leu Cys Pro Ile Val Cys Cys Asp

115 120 125

Asp Thr Met Arg Arg Arg Val Ala Gly Lys Lys Val His Trp Val Val

130 135 140

Ser Arg Ile Leu Ala Asp Leu His Gly Leu Thr Asn Asp Glu Ile Cys

145 150 155 160

His Val Thr Val Ala Tyr Glu Pro Ser Trp Ala Ile Gly Thr Gly Glu

165 170 175

Ser Ala Asp Pro Glu Gln Ala Ala Glu Gly Cys Tyr Leu Ile Arg Gln

180 185 190

Thr Ile Ser Asp Met Tyr Gly Asp Glu Val Ala Asn Asn Val Arg Ile

195 200 205

Leu Tyr Gly Gly Ser Val Thr Thr Ser Asn Ile Asn Ala Leu Met Ala

210 215 220

Lys Asn Asp Ile Asp Gly Val Leu Val Gly Ala Ala Ser Leu Asn Pro

225 230 235 240

Glu Thr Phe Leu Gln Leu Val His His

245

<210>48

<211>750

<212>DNA

<213>罗伊氏乳杆菌(Lactobacillus reuteri)

<400>48

atgagagtac cgattattgc tggtaattgg aaaatgcata aggatgtaca agaagctgtc 60

tcttttatcg aaaaagtaaa aaatcagctt ccgcctgccg accaacttga aacagcaatt 120

gctgctccta ctctttgttt agtaccaatg gttaaagcag ctgaagaatc cccgttaaaa 180

ataatggcag aaaactgcta ctataagaat gagggagctt atactggtga aacaagtcca 240

tatgctttat accaagcagg aatccatcat gtgattttag gccattctga acgccgaact 300

tactttaatg aaactgatga attaattaat aaaaaagtga aggcagcatt agtaaatggg 360

ttatgtccga ttgtttgttg tgatgatact atgcgtcgac gagttgctgg aaagaaagtt 420

cattgggtgg tgagccgaat tctcgctgac cttcatggat tgaccaatga cgaaatttgt 480

catgttacgg ttgcttatga accaagttgg gcgattggaa caggcgagag tgctgatcca 540

gaacaagcgg cggaaggttg ttaccttatt cggcaaacga ttagtgatat gtatggcgat 600

gaagttgcaa ataacgttcg aattctctat ggcggaagtg tgacaacttc taatatcaat 660

gcactaatgg caaaaaatga tattgatggt gttttagtcg gagcggcgag cttaaatcca 720

gaaacatttt tacaattagt tcaccattag 750

Claims

1.一种抗肥胖剂，其以属于罗伊氏乳杆菌的微生物为有效成分，该微生物产生由以下(1)～(3)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的脂肪酶。

(1)M V K L M T I H E L A N N P T L S G Q V R L I E N I V Y G A

M D G E A L H M S I L A P W T Q R F P K Q Y Q T E P R P L I

V F V Q G S S W R T P K M G E E I P Q L V Q F V R A G Y I V

A T V Q H R S S I D S H P F P A F L Q D V K T A I R F L R A

N A Q K Y A I D P Q Q V A I W G T S S G A N A A M L V G L T

G D D P R Y K V D L Y Q D E S D A V D A V V S C F A P M D V

E K T F E Y D A N V P G N K L L Q Y C L L G P D V S K W P E

I E K Q M S P L Y Q V K D G Q N Y P P F L L F H G D A D K V

V P Y E Q M E K M Y M R L K D N G N S V E A Y R V K G A N H

E R D F W S P T I Y N I V Q K F L G D Q F K

(2)L I Y V L K D L C N T I A E V Y G K S I L K G V F I M K H T

L K V D Q V R D G L W L D S D I T Y T Q V P G W L G N T T R

D L K L S V I R H F Q T N D D T R Y P V I F W F A G G G W M

D T D H N V H L P N L V D F A R H G Y I V V G V E Y R D S N

K V Q F P G Q L E D A K A A I R Y M R A N A K R F Q A D P N

R F I V M G E S A G G H M A S M L G V T N G L N Q F D K G A

N L D Y S S D V Q V A V P F Y G V V D P L T A K T G S A S N

D F D F V Y R N L L G A E P E N A P E L D S A A N P L T Y V

N S N S T P F L I F H G T E D V V V P I K D S E K L Y D A L

V E N N V P A E L Y E I E G A S H M D V K F L Q P Q V F K I

V M D F L D K Y L T R S

(3)M E I K S V N L D Q P Y S S L D I Y H S N T D K A L P G L V

I L P G G S Y N Q I M E R D S E R V A L T F A T H A W Q T F

V V R Y P V V E H K N Y E E A K I A V H Q A F E Y I V N H A

A E L D V D A D R L G I I G F S A G G Q I A A A Y S N E K L

T H A R F A A L G Y P V I Q P L I D E R M G V T T E N V A K

L V N P Q T P P T F M W G S A K D E L T P F V D H L Q V Y A

D A L I K N D I P Y E L H E F G T G G H G I A L A N E Y T G

I V N N D R V D N H M G K W F P L F L E W L T E L N L I

2.根据权利要求1所述的抗肥胖剂，其中，属于罗伊氏乳杆菌的微生物具有编码上述(1)～(3)的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列的核苷酸序列以及下述式(4)所示的核苷酸序列。

(4)ATGCATGGATTTATTGGCGAATTTTTTGGCACCATGGTTTTAATCCTATTAGGAGCAGGA

TGTTGTGCTGGTAATAGTTTGAATAAAACATATGGGAAACAAAGTGGCTGGTGGTTTATC

TGTATTTCATGGGGCTTAGCAGTTACAATGGGAGTTTATGTTGCAGGATTTCTGGGTTCA

TTAGGGCACTTAAATCCCGCTGTAACAATTCCTTTTGCTATTTTTGGCTTATTCCCATGG

AGTAACGTTATACCTTACTTACTTGGTCAATTTCTTGGTGCGTTTGTTGGTGCAGTATTA

GTAATTATTCAATTCTATCCACAATTTAAAGCAACCCCAAATGAAGAAGAAGGAAATAAT

GTTGGTATTTTTGCTACTCGTCCAGCGATAAATAGTCCAATTTTTAACTTTTTCTCAGAA

GTGATTGCGACCTTTGCATTTATTTTCATCTTATTAAATCTTGGCAACTTTACACAGGGA

TTGAAGCCATTTATCGTAGGAATGGTTATTGCAGTTGTTGGTACATGTCTCGGGACAACT

ACTGGCTTTGCATTAAACCCAGCTCGTGATTGGTCACCACGTTTAGCATATACTATTTTG

CCAATTCCTAATAAGGGTGTTTCAGAATGGTGGTATGCATGGGTTCCAATGTGTGGCCCA

ATTGTTGGGGGCCTTCTTGCTTGTGCTTTACAAACGGCACTAGTTTAG

3.根据权利要求1或2所述的抗肥胖剂，其中，属于罗伊氏乳杆菌的微生物进一步产生由下述(5)～(7)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的亚基所构成的甘油分解酶。

(5)M K R Q K R F E E L E K R P I H Q D T F V K E W P E E G F V

A M M G P N D P K P S V K V E N G K I V E M D G K K L E D F

D L I D L Y I A K Y G I N I D N V E K V M N M D S T K I A R

M L V D P N V S R D E I I E I T S A L T P A K A E E I I S K

L D F G E M I M A V K K M R P R R K P D N Q C H V T N T V D

N P V Q I A A D A A D A A L R G F P E Q E T T T A V A R Y A

P F N A I S I L I G A Q T G R P G V L T Q C S V E E A T E L

Q L G M R G F T A Y A E T I S V Y G T D R V F T D G D D T P

W S K G F L A S C Y A S R G L K M R F T S G A G S E V L M G

Y P E G K S M L Y L E A R C I L L T K A S G V Q G L Q N G A

V S C I E I P G A V P N G I R E V L G E N L L C M M C D I E

C A S G C D Q A Y S H S D M R R T E R F I G Q F I A G T D Y

I N S G Y S S T P N Y D N T F A G S N T D A M D Y D D M Y V

M E R D L G Q Y Y G I H P V K E E T I I K A R N K A A K A L

Q A V F E D L G L P K I T D E E V E A A T Y A N T H D D M P

K R D M V A D M K A A Q D M M D R G I T A I D I I K A L Y N

H G F K D V A E A I L N L Q K Q K V V G D Y L Q T S S I F D

K D W N V T S A V N D G N D Y Q G P G T G Y R L Y E D K E E

W D R I K D L P F A L D P E H L E L

(6)M A D I D E N L L R K I V K E V L S E T N Q I D T K I D F D

K S N D S T A T A T Q E V Q Q P N S K A V P E K K L D W F Q

P V G E A K P G Y S K D E V V I A V G P A F A T V L D K T E

T G I P H K E V L R Q V I A G I E E E G L K A R V V K V Y R

S S D V A F C A V Q G D H L S G S G I A I G I Q S K G T T V

I H Q K D Q D P L G N L E L F P Q A P V L T P E T Y R A I G

K N A A M Y A K G E S P E P V P A K N D Q L A R I H Y Q A I

S A I M H I R E T H Q V V V G K P E E E I K V T F D

(7)M S E V D D L V A K I M A Q M G N S S S A N S S T G T S T A

S T S K E M T A D D Y P L Y Q K H R D L V K T P K G H N L D

D I N L Q K V V N N Q V D P K E L R I T P E A L K L Q G E I

A A N A G R P A I Q K N L Q R A A E L T R V P D E R V L E M

Y D A L R P F R S T K Q E L L N I A K E L R D K Y D A N V C

A A W F E E A A D Y Y E S R K K L K G D N

4.根据权利要求1～3任一项所述的抗肥胖剂，其中，属于罗伊氏乳杆菌的微生物进一步还产生由下述(8)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的肠道黏附性蛋白质。

(8)M F G H D G R I V T K V Y Q W A G T Y Y Y F D P N T Y L R V

D N D Y R Q S Q W G D W Y M F G P D G R I V T G L K E W Y G

S Y Y Y F D P T T Y L K V T N K W I D N K Y F G P A G Q Q A

I S R F E R L D N K Y Y Y F D A N G A V L N I H D Q F K N I

D N H T Y Y F G A D G A C Y T S Q F L N K D G K Q Y Y F D N

D G I M L T D Q E K I I D G K F Y H F N V N G E A I Q V N D

P S E I

5.根据权利要求1～4任一项所述的抗肥胖剂，其中，属于罗伊氏乳杆菌的微生物进一步产生由下述(16)～(20)任一个所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的甘油分解酶。

(16)M K A A V I N D P V D G F V T V K D V Q L R D L K P G E A L

V D M E Y C G L C H T D L H V A A G D F G K K P G R I I G H

E G V G R V S K V A P G V T S L K V G D R V S I A W F F K G

C G H C E Y C L T G R E T L C R N V L N A G Y T A D G A M A

E Q C I V P A D Y A V K V P E G L D P V E A T S L T C A G V

T M Y K A L K V A D I K P G Q W V S I V G A G G L G N L G I

Q L A H N V F G A H V I A V D G N P D K L E A A K K N G A E

I L I N R H D G D V D K Q I Q E K V G G V H A A V V T A V S

A S A F D Q A V D S L R P D G K L V A V A L P Q G D M K L N

I A K T V L D G I I V A G S L V G T R Q D L A E C F Q F G A

E G K V H P I V K T R K L S E I N D M I Q E L K D N K V V G

R N V V D F V H N D N D

(17)M E K R E N A I P K T M K A W A V T T P G P I D G K E S P I

E F T E K P V P T P K R G E V L V K V I T C G V C H T D L H

V T E G D L P V H H E H V T P G H E I V G K V V G F G P E T

Q R F K F G E R I G I P W F R H A C G V C K F C R S G H E N

L C P H S L Y T G W D H D G G Y A E Y V T V P E G F A Y R L

P E K F D S L E A A P L L C A G I I G Y R A F E R A N V P A

G G R L G L Y G F G G S A H I T A Q I A L A Q G I E V H V F

T R G E D A K K F A L E L G C A S V Q G S Y D P A P V P L D

S S I I F A P V G D M V L P A L A S L V P G G T L A L A G I

H M T D I P T M N Y Q K E I F H E K T L T S V E S N T R R D

G E E F L T L A D R L N I H P E V H E Y P L A K A D E A L R

Y V K H G D I K G A C V L R V S E D

(18)M Q I K A A L A T K P N A D L E I Q T V E L D E P K E N E V

L I K I A S T G F C H T D I V G R S G A T T P L P V V L G H

E G A G V V Q K V G A N V T D V K P G D H V V L S F S Y C G

H C Y N C T H N H Q G L C E N F N Q L N F E G K T Y D G T H

R L H L D D G T P V S V F F G Q S S F A T Y V T A N V H N I

V K V D Q D V D L N L L G P L G C G M Q T G A G T V L N Y I

K P A P E D A I A V F G A G A V G L A A I M A A K I A G V K

H I I A I N R N G N H L D L A K E L G A T E T I N N T A E D

P V K A I K E I V P R G V T Y A I D T T G N T G V I K S A I

D S L A T A G E C V L L G V G G D I T L D L M N D I L S E S

K K I S G V V E G D S N P Q E F I P Q L V K Y Y K Q S K F P

L D K L V K Y Y D F A D I N Q V I A D S T N G K V I K P I I

K I D P E L A K L P L T N D G S N V Q K M V A E A G L A D Q

I T I D S A G T S N I A E G S P A D S R T K A I L D K Y H I

K D D G M I A R Q L Q D R D Y Y D A D Y I I A M D Q M N V R

D A K D M A P A G L E N K V H G I F E A T P G K E N C Y I V

D P W I T H

(19)M K K A I F E K A G Q M K I V D V D R P T I E K P D D V I I

K V V R T C V C G S D L W N F R G I N P V E K D S E N S G H

E A I G I V E E V G E D I T T V K P G D F V I A P F T H G C

G H C A A C R A G F D G S C Q S H N D N F S S G V Q A Q Y V

R F Q H G Q W A L V K V P G K P S D Y S E G M L K S L L T L

A D V M A T G Y H A A R V A N V S D G D T V V V M G D G A V

G L C A I I A A K M R G A K K I I S T S R H A D R Q A L A K

E F G A T D N V A E R S D E A V Q K I M E L T N G A G A D A

V L E C V G T E Q S T D T A M K V G R P G T I V G R V G L P

H T P K M D M T V L F Y N N T I V G G G P A S V T T Y D K D

V L L K A V L D G D I N P G K V F T K S F D L D Q I Q E A Y

E A M D K R E A I K S Y I I M D G F E R D

(20)M G R L D N K V A I I T G G S K G I G A A V A K K F I E E G

A K V V L T A R K M D E G Q K V A D Q L G D N A I F I Q Q D

V A R K G D W D R V I R Q T V Q V F G K L N I V V N N A G I

A E Y A D V E K T D A E I W D K T I A V N L T G T M W G T K

L G I E A M K N N G E K N S I I N M S S I E G L I G D P D L

F A Y N A S K G G V R L L T K S A A L D C A R K G Y D I R V

N T I H P G Y I S T P L V D N L V K D D P K A E G H L E S L

H P L G R L G K P E E I A N L A L Y L A S D E S S F S T G S

E F V A D G G Y T A Q

6.根据权利要求1～5任一项所述的抗肥胖剂，其中，属于罗伊氏乳杆菌的微生物进一步产生由下述(21)或(22)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的甘油分解酶。

(21)M T N V P T V K L N N G V E M P T L G F E V F Q V P D L S Q

A E Q A V T D A L E V G Y R L I D T A A A Y Q N E E A V G K

A I K N S S V N R E D V F V T S K L W V S D F N Y K R A K A

G I D A S L Q K L G L D Y M D L Y L L H Q P Y G D T M G A W

R A L Q E A Q K E G K I R A I G V S N F Y A D Q L K D L E L

T M P V K P A V N Q I E V N P W Y Q Q D Q E V K F A Q S E D

I R V E A W A P F A E G K H D I F T N E I I A E I A A K Y G

K S N G Q V I L R W L L Q R G I T V I P K S V H K N R M E E

N I D V F D F E L S N D D M K K I A S L N K K E S Q F F D H

R D P V T I E Q I F G S S L K M V Q D D E K

(22)M I L D E T I T L N S G V K I P K F A L G T W M I D D D Q A

A E A V R N A I K M G Y R H I D T A Q A Y D N E R G V G E G

V R T A G I D R D K I F V T S K I A A E H K D Y D V T K K S

I D E T L E K M G L D Y I D M M L I H S P Q P W K E V N Q S

D N R Y L E G N L A A W R A M E D A V N E G K I R T I G V S

N F K K A D L E N I I K N S D T V P A V D Q V L A H I G H T

P F N L L S F T H E H D I A V E A Y S P V A H G A A L D N P

V I E K M A K K Y N V S V P Q L C I R Y D W Q I G M I V L P

K T T N P E H M K E N T E I D F E I S E A D M D L L R R V K

P L D Y G D F D I Y P V Y G G K M

7.根据权利要求1～6任一项所述的抗肥胖剂，其中，属于罗伊氏乳杆菌的微生物进一步产生由下述(23)～(25)任一个所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的醛脱氢酶。

(23)M P A N N K K Q V E K K E L T A E E K K Q N A Q K L V D D L

M T K S Q A A F E K L R Y Y S Q E Q V D K I C Q A M A L A A

E E H H M D L A V D A A N E T G R G V A E D K A I K N I Y A

S E Y I W N N I R H D K T V G I I E D N D E D Q T I K I A D

P L G V I A G I V P V T N P T S T T I F K S I I S A K T R N

T I I F S F H R Q A M K S S I K T A K I L Q E A A E K A G A

P K N M I Q W L P E S T R E N T T A L L Q H P N T A T I L A

T G G P S L V K A A Y S S G N P A L G V G P G N G P A Y I E

K T A N I E R S V Y D I V L S K T F D N G M I C A T E N S V

V V D E E I Y D K V K E E F Q K W N C Y F L K P N E I D K F

T D G F I D P D R H Q V R G P I A G R S A N A I A D M C G I

K V P D N T K V I I A E Y E G V G D K Y P L S A E K L S P V

L T M Y K A T S H E N A F D I C A Q L L H Y G G E G H T A A

I H T L D D D L A T K Y G L E M R A S R I I V N S P S G I G

G I G N I Y N N M T P S L T L G T G S Y G S N S I S H N V T

D W D L L N I K T I A K R R E N R Q W V K I P P K V Y F Q R

N S L K E L Q D I P N I N R A F I V T G P G M S K R G Y V Q

R V I D Q L R Q R Q N N T A F L V F D D V E E D P S T N T V

E K G V A M M N D F K P D T I I A L G G G S P M D A A K A M

W M F Y E H P E T S W Y G V M Q K Y L D I R K R A Y Q I K K

P T K S Q L I G I P T T S G T G S E V T P F A V I T D S K T

H V K Y P L A D Y A L T P N I A I V D S Q F V E T V P A K T

T A W T G L D V L C H A T E S Y V S V M A T D Y T R G W S L

Q T I K G V M E N L P K S V Q G D K L A R R K M H D F S T M

A G M A F G Q A F L G I N H S L A H K M G G A F G L P H G L

L I A I A M P Q V I R F N A K R P Q K L A L W P H Y E T Y H

A T K D Y A D I A R F I G L K G N T D E E L A E A Y A K K V

I E L A H E C G V K L S L K D N G V T R E E F D K A V D D L

A R L A Y E D Q C T T T N P V E P L V S Q L K E L L E R C Y

D G T G V E E K

(24)M A Y Q S I N P F T N Q V E K T F E N T T D E E L E Q T L T

T A H Q L Y L D W R K Y N D L E E R K R Q I L K L G Q I L R

E R R V E Y A T V M S K E M G K L I S E A E G E V D L C A S

F C D Y Y A A H A D E F L Q P K I I A T T S G R A K V L K Q

S L G I L V A V E P W N F P F Y Q I A R V F I P N F I A G N

P M I L K D A S N C P A S A Q A F N D A V K E A G A P A G S

L T N L F L S Y D Q V N K A I A D K R V A G V C L T G S E R

G G A T V A K E A G A N L K K S T L E L G G N D A F I I L D

D A D W D L V E K V A P A A R L Y N A G Q V C T S S K R F I

V L E K D Y D R F L K M M K D A F S K V K M G D P L D P L T

T L A P L S S K K A K E K L Q Q Q V A T A V E N G A K V Y Y

G N K P V D M E G Q F F M P T I L T D I T P D N P I F D T E

M F G P V A S V Y K V S S E E E A I E L A N N S S Y G L G N

T I F S N D S E H A E R V A A K I E T G M S W I N A G W A S

L P E L P F G G V K N S G Y G R E L S S Y G I D E F T N K H

L I Y E A R Q

(25)M Q I N D I E S A V R K I L A E E L D N A S S S S A N V A A

T T D N G H R G I F T N V N D A I A A A K A A Q E I Y R D K

P I A V R Q Q V I D A I K E G F R P Y I E K M A K D I K E E

T G M G T V E A K I A K L N N A L Y N T P G P E I L E P V V

E N G D G G M V M Y E R L P Y G V I G A V G P S T N P S E T

V I A N A I M M L A G G N T L Y F G A H P G A K N V T R W T

I E K M N D F I A D A T G L H N L V V S I E T P T I E S V Q

Q M M K H P D I A M L A V T G G P A V V H Q A M T S G K K A

V G A G P G N P P A M V D A T A D I D L A A H N I I T S A S

F D N D I L C T A E K E V V A E S S I K D E L I R K M Q D E

G A F V V N R E Q A D K L A D M C I Q E N G A P D R K F V G

K D A T Y I L D Q A N I P Y T G H P V E I I C E L P K E H P

L V M T E M L M P I L P V V S C P T F D D V L K T A V E V E

K G N H H T A T I H S N N L K H I N N A A H R M Q C S I F V

V N G P S Y V G T G V A D N G A H S G A S A L T I A T P T G

E G T C T A R T F T R R V R L N S P Q G F S V R N W Y

8.根据权利要求1～7任一项所述的抗肥胖剂，其中，属于罗伊氏乳杆菌的微生物进一步产生由下述(26)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的甘油酸激酶。

(26)M K F V I A P D S F K G G L T A K E A A N V M A E G I K R V

F P N A E Y A L V P M A D G G E G T V Q S L V D A T N G Q K

M I A K V H N P L N K L V N A E Y G I L G D G E T A V I E M

A A A S G L Q F V N K E T A N P L I T T T Y G T G E L I K D

A L D H N I K K I I I G I G G S A T V D G G A G M A Q A L G

A R L L D A D N H E I G L G G G E L A S L E Q V D F G G L D

P R L K N V D I Q I A S D V T N P L T G K N G A A P V F G P

Q K G A D E E M V N I L D K N L H H Y A R K I V A A G G P D

V E Q T A G A G A A G G L G A G L I A F T G A T M K R G V E

L V I E A T Q L Q K K A V G A D Y V F T G E G G I D F Q T K

F G K T P Y G V A K A T K E V A P T A P V I V L A G N I G K

G V N D L Y S S T A I D A I F A T P E G A K P L K T A L A D

A P I D I A Q T A E N V A R L I K V S H V S N

9.根据权利要求1～8任一项所述的抗肥胖剂，其中，属于罗伊氏乳杆菌的微生物进一步产生由下述(27)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的甘油激酶。

(27)L S E Q Q Y I M A I D Q G T T S S R A I I F D H D G N K V A

I S Q Q E F P Q Y F P Q P G W V E H D P L E I W D S V Q S V

I S N V M I K S Q I K P Y K I A A I G I T N Q R E T T V I W

D R H T G K P I Y N A I V W Q S K Q T S D I A E Q L I K D G

Y K D M I H Q K T G L V I D S Y F A A T K I K W I L D H V P

G A R E K A A K G D L M F G T I D T W L L W N L S G R R V H

A T D V T N A S R T M L F N I H T L D W D Q D I L D L L D I

P Q S L L P V V K P S S A I Y G Y T G D Y H F Y G V Q I P I

A G I A G D Q Q A A L F G Q A A Y D K G S I K N T Y G T G A

F I V M N T G L K P T L S D N G L L T T I A Y G L D G Q T H

Y A L E G S I F V A G S A V Q W L R D G L K M F D K A S E S

E Q M A V D A K T T G G V Y V V P A F T G L G A P Y W D Q E

V R G A M F G L T R G T E R G H I I R A T L E A I A Y Q T K

D V V D T M V K D T Q L P L T A L T V N G G A S R N N F M M

Q F Q A D I L Q T P I K R A A M E E T T A L G A A F L A G L

A V D F W E D Q D E L R K L S R I G D Q F D P Q M D P Q K A

A D L Y R G W Q R A I A A A Q F Y G K D

10.根据权利要求1～9任一项所述的抗肥胖剂，其中，属于罗伊氏乳杆菌的微生物进一步产生由下述(28)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的甘油-3-磷酸脱氢酶。

(28)M A E K I A V L G A G S W G S V L A N M L T E N G H D V T L

W S R N E E Q V K Q L N T E H T N P R Y M K D F V Y S T N L

T A T T D M K K A V K G A S V V L I V I P T K G L R E V A K

Q L N A I L T E L H Q K P L V I H A T K G L E Q N T Y K R P

S E M L S E D I S P E N R Q A I V V L S G P S H A E D V A I

K D M T A V T A A C E D L A S A K K A Q K L F S N S Y F R V

Y T N D D V I G A E F G A A L K N I I A I G A G A I Q G L G

Y H D N A R A A L I T R G L A E I R R L G V A F G A N P M T

F I G L S G V G D L V V T A T S K N S R N W R A G Y Q L G Q

G K K L Q D V I D N M G M V I E G V Y T T K A A Y E L S R K

R Q V Q M P I T E A L Y R V L Y E G E D I K T A I S Q L M D

R D L T S E N E

11.根据权利要求1～10任一项所述的抗肥胖剂，其中，属于罗伊氏乳杆菌的微生物进一步产生由下述(29)～(31)任一个所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的磷酸丙糖异构酶。

(29)M R K P F I A A N W K M H K N V Q E S V E F V D A I K G K L

P D P Q E V E V G I A A Q A F A L P S M V Q A A D D S G L K

I I A Q N A A A E Y S G A F T G E I S L R G L A D A G V S Y

V M L G H I E R R H L F H E D N E L V N R K V L A A L Q M G

V T P I I C T D E T M V Q K E V N G E I H Y V F Q Q L M S V

L R G V S L D Q I K N V V V S Y E P S W A V G Y G Q H A N P

V L A E E G C R Q I R R T I A D N Y T Y E I A D K I R I L Y

G G S V N P D N I G M I M N K P D V D G V L I G R A S L D V

D N F L R M V N Y L K N D Q E K

(30)M R K P F I I A N W K M N K N V H E S V A F V K A I K E K L

P A D K E I G I A A Q A V S L Y N M K K V A S S S N L Q I I

A Q N A S A E L E G P Y T G E I S M R S L A D A G V T Y V M

L G H L E R R R L F N E S N D S I N Q K V L A A L N A G I I

P I I C T D E E M V Q T E V N G Q I H Y V F R Q L K S V L K

G V P A N K L S Q I V I S Y E P S W A V G S T H Q A N P D I

A E E G C Q A I R Q S L V E M Y G N E I G E Q V R I L Y G G

S V N P E N I G Q I M S K P N V D G A L I G R A S L E I E S

F L Q M I N Y I E L A S K Q K L Q V I

(31)M R V P I I A G N W K M H K D V Q E A V S F I E K V K N Q L

P P A D Q L E T A I A A P T L C L V P M V K A A E E S P L K

I M A E N C Y Y K N E G A Y T G E T S P Y A L Y Q A G I H H

V I L G H S E R R T Y F N E T D E L I N K K V K A A L V N G

L C P I V C C D D T M R R R V A G K K V H W V V S R I L A D

L H G L T N D E I C H V T V A Y E P S W A I G T G E S A D P

E Q A A E G C Y L I R Q T I S D M Y G D E V A N N V R I L Y

G G S V T T S N I N A L M A K N D I D G V L V G A A S L N P

E T F L Q L V H H

12.根据权利要求1～11任一项所述的抗肥胖剂，其中，属于罗伊氏乳杆菌的微生物为罗伊氏乳杆菌JCM1112T。

13.一种抗肥胖饮食品，其以属于罗伊氏乳杆菌的微生物为有效成分，该微生物产生由以下(1)～(3)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的脂肪酶。

(1)M V K L M T I H E L A N N P T L S G Q V R L I E N I V Y G A

M D G E A L H M S I L A P W T Q R F P K Q Y Q T E P R P L I

V F V Q G S S W R T P K M G E E I P Q L V Q F V R A G Y I V

A T V Q H R S S I D S H P F P A F L Q D V K T A I R F L R A

N A Q K Y A I D P Q Q V A I W G T S S G A N A A M L V G L T

G D D P R Y K V D L Y Q D E S D A V D A V V S C F A P M D V

E K T F E Y D A N V P G N K L L Q Y C L L G P D V S K W P E

I E K Q M S P L Y Q V K D G Q N Y P P F L L F H G D A D K V

V P Y E Q M E K M Y M R L K D N G N S V E A Y R V K G A N H

E R D F W S P T I Y N I V Q K F L G D Q F K

(2)L I Y V L K D L C N T I A E V Y G K S I L K G V F I M K H T

L K V D Q V R D G L W L D S D I T Y T Q V P G W L G N T T R

D L K L S V I R H F Q T N D D T R Y P V I F W F A G G G W M

D T D H N V H L P N L V D F A R H G Y I V V G V E Y R D S N

K V Q F P G Q L E D A K A A I R Y M R A N A K R F Q A D P N

R F I V M G E S A G G H M A S M L G V T N G L N Q F D K G A

N L D Y S S D V Q V A V P F Y G V V D P L T A K T G S A S N

D F D F V Y R N L L G A E P E N A P E L D S A A N P L T Y V

N S N S T P F L I F H G T E D V V V P I K D S E K L Y D A L

V E N N V P A E L Y E I E G A S H M D V K F L Q P Q V F K I

V M D F L D K Y L T R S

(3)M E I K S V N L D Q P Y S S L D I Y H S N T D K A L P G L V

I L P G G S Y N Q I M E R D S E R V A L T F A T H A W Q T F

V V R Y P V V E H K N Y E E A K I A V H Q A F E Y I V N H A

A E L D V D A D R L G I I G F S A G G Q I A A A Y S N E K L

T H A R F A A L G Y P V I Q P L I D E R M G V T T E N V A K

L V N P Q T P P T F M W G S A K D E L T P F V D H L Q V Y A

D A L I K N D I P Y E L H E F G T G G H G I A L A N E Y T G

I V N N D R V D N H M G K W F P L F L E W L T E L N L I

14.根据权利要求13所述的抗肥胖饮食品，其中，属于罗伊氏乳杆菌的微生物具有编码上述(1)～(3)的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列的核苷酸序列以及下述式(4)所示的核苷酸序列。

(4)ATGCATGGATTTATTGGCGAATTTTTTGGCACCATGGTTTTAATCCTATTAGGAGCAGGA

TGTTGTGCTGGTAATAGTTTGAATAAAACATATGGGAAACAAAGTGGCTGGTGGTTTATC

TGTATTTCATGGGGCTTAGCAGTTACAATGGGAGTTTATGTTGCAGGATTTCTGGGTTCA

TTAGGGCACTTAAATCCCGCTGTAACAATTCCTTTTGCTATTTTTGGCTTATTCCCATGG

AGTAACGTTATACCTTACTTACTTGGTCAATTTCTTGGTGCGTTTGTTGGTGCAGTATTA

GTAATTATTCAATTCTATCCACAATTTAAAGCAACCCCAAATGAAGAAGAAGGAAATAAT

GTTGGTATTTTTGCTACTCGTCCAGCGATAAATAGTCCAATTTTTAACTTTTTCTCAGAA

GTGATTGCGACCTTTGCATTTATTTTCATCTTATTAAATCTTGGCAACTTTACACAGGGA

TTGAAGCCATTTATCGTAGGAATGGTTATTGCAGTTGTTGGTACATGTCTCGGGACAACT

ACTGGCTTTGCATTAAACCCAGCTCGTGATTGGTCACCACGTTTAGCATATACTATTTTG

CCAATTCCTAATAAGGGTGTTTCAGAATGGTGGTATGCATGGGTTCCAATGTGTGGCCCA

ATTGTTGGGGGCCTTCTTGCTTGTGCTTTACAAACGGCACTAGTTTAG

15.根据权利要求13或14所述的抗肥胖饮食品，其中，属于罗伊氏乳杆菌的微生物进一步产生由下述(5)～(7)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的亚基所构成的甘油分解酶。

(5)M K R Q K R F E E L E K R P I H Q D T F V K E W P E E G F V

A M M G P N D P K P S V K V E N G K I V E M D G K K L E D F

D L I D L Y I A K Y G I N I D N V E K V M N M D S T K I A R

M L V D P N V S R D E I I E I T S A L T P A K A E E I I S K

L D F G E M I M A V K K M R P R R K P D N Q C H V T N T V D

N P V Q I A A D A A D A A L R G F P E Q E T T T A V A R Y A

P F N A I S I L I G A Q T G R P G V L T Q C S V E E A T E L

Q L G M R G F T A Y A E T I S V Y G T D R V F T D G D D T P

W S K G F L A S C Y A S R G L K M R F T S G A G S E V L M G

Y P E G K S M L Y L E A R C I L L T K A S G V Q G L Q N G A

V S C I E I P G A V P N G I R E V L G E N L L C M M C D I E

C A S G C D Q A Y S H S D M R R T E R F I G Q F I A G T D Y

I N S G Y S S T P N Y D N T F A G S N T D A M D Y D D M Y V

M E R D L G Q Y Y G I H P V K E E T I I K A R N K A A K A L

Q A V F E D L G L P K I T D E E V E A A T Y A N T H D D M P

K R D M V A D M K A A Q D M M D R G I T A I D I I K A L Y N

H G F K D V A E A I L N L Q K Q K V V G D Y L Q T S S I F D

K D W N V T S A V N D G N D Y Q G P G T G Y R L Y E D K E E

W D R I K D L P F A L D P E H L E L

(6)M A D I D E N L L R K I V K E V L S E T N Q I D T K I D F D

K S N D S T A T A T Q E V Q Q P N S K A V P E K K L D W F Q

P V G E A K P G Y S K D E V V I A V G P A F A T V L D K T E

T G I P H K E V L R Q V I A G I E E E G L K A R V V K V Y R

S S D V A F C A V Q G D H L S G S G I A I G I Q S K G T T V

I H Q K D Q D P L G N L E L F P Q A P V L T P E T Y R A I G

K N A A M Y A K G E S P E P V P A K N D Q L A R I H Y Q A I

S A I M H I R E T H Q V V V G K P E E E I K V T F D

(7)M S E V D D L V A K I M A Q M G N S S S A N S S T G T S T A

S T S K E M T A D D Y P L Y Q K H R D L V K T P K G H N L D

D I N L Q K V V N N Q V D P K E L R I T P E A L K L Q G E I

A A N A G R P A I Q K N L Q R A A E L T R V P D E R V L E M

Y D A L R P F R S T K Q E L L N I A K E L R D K Y D A N V C

A A W F E E A A D Y Y E S R K K L K G D N

16.根据权利要求13～15任一项所述的抗肥胖饮食品，其中，属于罗伊氏乳杆菌的微生物进一步产生由下述(8)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的肠道黏附性蛋白质。

(8)M F G H D G R I V T K V Y Q W A G T Y Y Y F D P N T Y L R V

D N D Y R Q S Q W G D W Y M F G P D G R I V T G L K E W Y G

S Y Y Y F D P T T Y L K V T N K W I D N K Y F G P A G Q Q A

I S R F E R L D N K Y Y Y F D A N G A V L N I H D Q F K N I

D N H T Y Y F G A D G A C Y T S Q F L N K D G K Q Y Y F D N

D G I M L T D Q E K I I D G K F Y H F N V N G E A I Q V N D

P S E I

17.根据权利要求13～16任一项所述的抗肥胖饮食品，其中，属于罗伊氏乳杆菌的微生物进一步产生由下述(16)～(20)任一个所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的甘油分解酶。

(16)M K A A V I N D P V D G F V T V K D V Q L R D L K P G E A L

V D M E Y C G L C H T D L H V A A G D F G K K P G R I I G H

E G V G R V S K V A P G V T S L K V G D R V S I A W F F K G

C G H C E Y C L T G R E T L C R N V L N A G Y T A D G A M A

E Q C I V P A D Y A V K V P E G L D P V E A T S L T C A G V

T M Y K A L K V A D I K P G Q W V S I V G A G G L G N L G I

Q L A H N V F G A H V I A V D G N P D K L E A A K K N G A E

I L I N R H D G D V D K Q I Q E K V G G V H A A V V T A V S

A S A F D Q A V D S L R P D G K L V A V A L P Q G D M K L N

I A K T V L D G I I V A G S L V G T R Q D L A E C F Q F G A

E G K V H P I V K T R K L S E I N D M I Q E L K D N K V V G

R N V V D F V H N D N D

(17)M E K R E N A I P K T M K A W A V T T P G P I D G K E S P I

E F T E K P V P T P K R G E V L V K V I T C G V C H T D L H

V T E G D L P V H H E H V T P G H E I V G K V V G F G P E T

Q R F K F G E R I G I P W F R H A C G V C K F C R S G H E N

L C P H S L Y T G W D H D G G Y A E Y V T V P E G F A Y R L

P E K F D S L E A A P L L C A G I I G Y R A F E R A N V P A

G G R L G L Y G F G G S A H I T A Q I A L A Q G I E V H V F

T R G E D A K K F A L E L G C A S V Q G S Y D P A P V P L D

S S I I F A P V G D M V L P A L A S L V P G G T L A L A G I

H M T D I P T M N Y Q K E I F H E K T L T S V E S N T R R D

G E E F L T L A D R L N I H P E V H E Y P L A K A D E A L R

Y V K H G D I K G A C V L R V S E D

(18)M Q I K A A L A T K P N A D L E I Q T V E L D E P K E N E V

L I K I A S T G F C H T D I V G R S G A T T P L P V V L G H

E G A G V V Q K V G A N V T D V K P G D H V V L S F S Y C G

H C Y N C T H N H Q G L C E N F N Q L N F E G K T Y D G T H

R L H L D D G T P V S V F F G Q S S F A T Y V T A N V H N I

V K V D Q D V D L N L L G P L G C G M Q T G A G T V L N Y I

K P A P E D A I A V F G A G A V G L A A I M A A K I A G V K

H I I A I N R N G N H L D L A K E L G A T E T I N N T A E D

P V K A I K E I V P R G V T Y A I D T T G N T G V I K S A I

D S L A T A G E C V L L G V G G D I T L D L M N D I L S E S

K K I S G V V E G D S N P Q E F I P Q L V K Y Y K Q S K F P

L D K L V K Y Y D F A D I N Q VIA D S T N G K V I K P I I

K I D P E L A K L P L T N D G S N V Q K M V A E A G L A D Q

I T I D S A G T S N I A E G S P A D S R T K A I L D K Y H I

K D D G M I A R Q L Q D R D Y Y D A D Y I I A M D Q M N V R

D A K D M A P A G L E N K V H G I F E A T P G K E N C Y I V

D P W I T H

(19)M K K A I F E K A G Q M K I V D V D R P T I E K P D D V I I

K V V R T C V C G S D L W N F R G I N P V E K D S E N S G H

E A I G I V E E V G E D I T T V K P G D F V I A P F T H G C

G H C A A C R A G F D G S C Q S H N D N F S S G V Q A Q Y V

R F Q H G Q W A L V K V P G K P S D Y S E G M L K S L L T L

A D V M A T G Y H A A R V A N V S D G D T V V V M G D G A V

G L C A I I A A K M R G A K K I I S T S R H A D R Q A L A K

E F G A T D N V A E R S D E A V Q K I M E L T N G A G A D A

V L E C V G T E Q S T D T A M K V G R P G T I V G R V G L P

H T P K M D M T V L F Y N N T I V G G G P A S V T T Y D K D

V L L K A V L D G D I N P G K V F T K S F D L D Q I Q E A Y

E A M D K R E A I K S Y I I M D G F E R D

(20)M G R L D N K V A I I T G G S K G I G A A V A K K F I E E G

A K V V L T A R K M D E G Q K V A D Q L G D N A I F I Q Q D

V A R K G D W D R V I R Q T V Q V F G K L N I V V N N A G I

A E Y A D V E K T D A E I W D K T I A V N L T G T M W G T K

L G I E A M K N N G E K N S I I N M S S I E G L I G D P D L

F A Y N A S K G G V R L L T K S A A L D C A R K G Y D I R V

N T I H P G Y I S T P L V D N L V K D D P K A E G H L E S L

H P L G R L G K P E E I A N L A L Y L A S D E S S F S T G S

E F V A D G G Y T A Q

18.根据权利要求13～17任一项所述的抗肥胖饮食品，其中，属于罗伊氏乳杆菌的微生物进一步产生由下述(21)或(22)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的甘油分解酶。

(21)M T N V P T V K L N N G V E M P T L G F E V F Q V P D L S Q

A E Q A V T D A L E V G Y R L I D T A A A Y Q N E E A V G K

A I K N S S V N R E D V F V T S K L W V S D F N Y K R A K A

G I D A S L Q K L G L D Y M D L Y L L H Q P Y G D T M G A W

R A L Q E A Q K E G K I R A I G V S N F Y A D Q L K D L E L

T M P V K P A V N Q I E V N P W Y Q Q D Q E V K F A Q S E D

I R V E A W A P F A E G K H D I F T N E I I A E I A A K Y G

K S N G Q V I L R W L L Q R G I T V I P K S V H K N R M E E

N I D V F D F E L S N D D M K K I A S L N K K E S Q F F D H

R D P V T I E Q I F G S S L K M V Q D D E K

(22)M I L D E T I T L N S G V K I P K F A L G T W M I D D D Q A

A E A V R N A I K M G Y R H I D T A Q A Y D N E R G V G E G

V R T A G I D R D K I F V T S K I A A E H K D Y D V T K K S

I D E T L E K M G L D Y I D M M L I H S P Q P W K E V N Q S

D N R Y L E G N L A A W R A M E D A V N E G K I R T I G V S

N F K K A D L E N I I K N S D T V P A V D Q V L A H I G H T

P F N L L S F T H E H D I A V E A Y S P V A H G A A L D N P

V I E K M A K K Y N V S V P Q L C I R Y D W Q I G M I V L P

K T T N P E H M K E N T E I D F E I S E A D M D L L R R V K

P L D Y G D F D I Y P V Y G G K M

19.根据权利要求13～18任一项所述的抗肥胖饮食品，其中，属于罗伊氏乳杆菌的微生物进一步产生由下述(23)～(25)任一个所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的醛脱氢酶。

(23)M P A N N K K Q V E K K E L T A E E K K Q N A Q K L V D D L

M T K S Q A A F E K L R Y Y S Q E Q V D K I C Q A M A L A A

E E H H M D L A V D A A N E T G R G V A E D K A I K N I Y A

S E Y I W N N I R H D K T V G I I E D N D E D Q T I K I A D

P L G V I A G I V P V T N P T S T T I F K S I I S A K T R N

T I I F S F H R Q A M K S S I K T A K I L Q E A A E K A G A

P K N M I Q W L P E S T R E N T T A L L Q H P N T A T I L A

T G G P S L V K A A Y S S G N P A L G V G P G N G P A Y I E

K T A N I E R S V Y D I V L S K T F D N G M I C A T E N S V

V V D E E I Y D K V K E E F Q K W N C Y F L K P N E I D K F

T D G F I D P D R H Q V R G P I A G R S A N A I A D M C G I

K V P D N T K V I I A E Y E G V G D K Y P L S A E K L S P V

L T M Y K A T S H E N A F D I C A Q L L H Y G G E G H T A A

I H T L D D D L A T K Y G L E M R A S R I I V N S P S G I G

G I G N I Y N N M T P S L T L G T G S Y G S N S I S H N V T

D W D L L N I K T I A K R R E N R Q W V K I P P K V Y F Q R

N S L K E L Q D I P N I N R A F I V T G P G M S K R G Y V Q

R V I D Q L R Q R Q N N T A F L V F D D V E E D P S T N T V

E K G V A M M N D F K P D T I I A L G G G S P M D A A K A M

W M F Y E H P E T S W Y G V M Q K Y L D I R K R A Y Q I K K

P T K S Q L I G I P T T S G T G S E V T P F A V I T D S K T

H V K Y P L A D Y A L T P N I A I V D S Q F V E T V P A K T

T A W T G L D V L C H A T E S Y V S V M A T D Y T R G W S L

Q T I K G V M E N L P K S V Q G D K L A R R K M H D F S T M

A G M A F G Q A F L G I N H S L A H K M G G A F G L P H G L

L I A I A M P Q V I R F N A K R P Q K L A L W P H Y E T Y H

A T K D Y A D I A R F I G L K G N T D E E L A E A Y A K K V

I E L A H E C G V K L S L K D N G V T R E E F D K A V D D L

A R L A Y E D Q C T T T N P V E P L V S Q L K E L L E R C Y

D G T G V E E K

(24)M A Y Q S I N P F T N Q V E K T F E N T T D E E L E Q T L T

T A H Q L Y L D W R K Y N D L E E R K R Q I L K L G Q I L R

E R R V E Y A T V M S K E M G K L I S E A E G E V D L C A S

F C D Y Y A A H A D E F L Q P K I I A T T S G R A K V L K Q

S L G I L V A V E P W N F P F Y Q I A R V F I P N F I A G N

P M I L K D A S N C P A S A Q A F N D A V K E A G A P A G S

L T N L F L S Y D Q V N K A I A D K R V A G V C L T G S E R

G G A T V A K E A G A N L K K S T L E L G G N D A F I I L D

D A D W D L V E K V A P A A R L Y N A G Q V C T S S K R F I

V L E K D Y D R F L K M M K D A F S K V K M G D P L D P L T

T L A P L S S K K A K E K L Q Q Q V A T A V E N G A K V Y Y

G N K P V D M E G Q F F M P T I L T D I T P D N P I F D T E

M F G P V A S V Y K V S S E E E A I E L A N N S S Y G L G N

T I F S N D S E H A E R V A A K I E T G M S W I N A G W A S

L P E L P F G G V K N S G Y G R E L S S Y G I D E F T N K H

L I Y E A R Q

(25)M Q I N D I E S A V R K I L A E E L D N A S S S S A N V A A

T T D N G H R G I F T N V N D A I A A A K A A Q E I Y R D K

P I A V R Q Q V I D A I K E G F R P Y I E K M A K D I K E E

T G M G T V E A K I A K L N N A L Y N T P G P E I L E P V V

E N G D G G M V M Y E R L P Y G V I G A V G P S T N P S E T

V I A N A I M M L A G G N T L Y F G A H P G A K N V T R W T

I E K M N D F I A D A T G L H N L V V S I E T P T I E S V Q

Q M M K H P D I A M L A V T G G P A V V H Q A M T S G K K A

V G A G P G N P P A M V D A T A D I D L A A H N I I T S A S

F D N D I L C T A E K E V V A E S S I K D E L I R K M Q D E

G A F V V N R E Q A D K L A D M C I Q E N G A P D R K F V G

K D A T Y I L D Q A N I P Y T G H P V E I I C E L P K E H P

L V M T E M L M P I L P V V S C P T F D D V L K T A V E V E

K G N H H T A T I H S N N L K H I N N A A H R M Q C S I F V

V N G P S Y V G T G V A D N G A H S G A S A L T I A T P T G

E G T C T A R T F T R R V R L N S P Q G F S V R N W Y

20.根据权利要求13～19任一项所述的抗肥胖饮食品，其中，属于罗伊氏乳杆菌的微生物进一步产生由下述(26)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的甘油酸激酶。

(26)M K F V I A P D S F K G G L T A K E A A N V M A E G I K R V

F P N A E Y A L V P M A D G G E G T V Q S L V D A T N G Q K

M I A K V H N P L N K L V N A E Y G I L G D G E T A V I E M

A A A S G L Q F V N K E T A N P L I T T T Y G T G E L I K D

A L D H N I K K I I I G I G G S A T V D G G A G M A Q A L G

A R L L D A D N H E I G L G G G E L A S L E Q V D F G G L D

P R L K N V D I Q I A S D V T N P L T G K N G A A P V F G P

Q K G A D E E M V N I L D K N L H H Y A R K I V A A G G P D

V E Q T A G A G A A G G L G A G L I A F T G A T M K R G V E

L V I E A T Q L Q K K A V G A D Y V F T G E G G I D F Q T K

F G K T P Y G V A K A T K E V A P T A P V I V L A G N I G K

G V N D L Y S S T A I D A I F A T P E G A K P L K T A L A D

A P I D I A Q T A E N V A R L I K V S H V S N

21.根据权利要求13～20任一项所述的抗肥胖饮食品，其中，属于罗伊氏乳杆菌的微生物进一步产生由下述(27)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的甘油激酶。

(27)L S E Q Q Y I M A I D Q G T T S S R A I I F D H D G N K V A

I S Q Q E F P Q Y F P Q P G W V E H D P L E I W D S V Q S V

I S N V M I K S Q I K P Y K I A A I G I T N Q R E T T V I W

D R H T G K P I Y N A I V W Q S K Q T S D I A E Q L I K D G

Y K D M I H Q K T G L V I D S Y F A A T K I K W I L D H V P

G A R E K A A K G D L M F G T I D T W L L W N L S G R R V H

A T D V T N A S R T M L F N I H T L D W D Q D I L D L L D I

P Q S L L P V V K P S S A I Y G Y T G D Y H F Y G V Q I P I

A G I A G D Q Q A A L F G Q A A Y D K G S I K N T Y G T G A

F I V M N T G L K P T L S D N G L L T T I A Y G L D G Q T H

Y A L E G S I F V A G S A V Q W L R D G L K M F D K A S E S

E Q M A V D A K T T G G V Y V V P A F T G L G A P Y W D Q E

V R G A M F G L T R G T E R G H I I R A T L E A I A Y Q T K

D V V D T M V K D T Q L P L T A L T V N G G A S R N N F M M

Q F Q A D I L Q T P I K R A A M E E T T A L G A A F L A G L

A V D F W E D Q D E L R K L S R I G D Q F D P Q M D P Q K A

A D L Y R G W Q R A I A A A Q F Y G K D

22.根据权利要求13～21任一项所述的抗肥胖饮食品，其中，属于罗伊氏乳杆菌的微生物进一步产生由下述(28)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的甘油-3-磷酸脱氢酶。

(28)M A E K I A V L G A G S W G S V L A N M L T E N G H D V T L

W S R N E E Q V K Q L N T E H T N P R Y M K D F V Y S T N L

T A T T D M K K A V K G A S V V L I V I P T K G L R E V A K

Q L N A I L T E L H Q K P L V I H A T K G L E Q N T Y K R P

S E M L S E D I S P E N R Q A I V V L S G P S H A E D V A I

K D M T A V T A A C E D L A S A K K A Q K L F S N S Y F R V

Y T N D D V I G A E F G A A L K N I I A I G A G A I Q G L G

Y H D N A R A A L I T R G L A E I R R L G V A F G A N P M T

F I G L S G V G D L V V T A T S K N S R N W R A G Y Q L G Q

G K K L Q D V I D N M G M V I E G V Y T T K A A Y E L S R K

R Q V Q M P I T E A L Y R V L Y E G E D I K T A I S Q L M D

R D L T S E N E

23.根据权利要求1 3～22任一项所述的抗肥胖饮食品，其中，属于罗伊氏乳杆菌的微生物进一步产生由下述(29)～(31)任一个所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的磷酸丙糖异构酶。

(29)M R K P F I A A N W K M H K N V Q E S V E F V D A I K G K L

P D P Q E V E V G I A A Q A F A L P S M V Q A A D D S G L K

I I A Q N A A A E Y S G A F T G E I S L R G L A D A G V S Y

V M L G H I E R R H L F H E D N E L V N R K V L A A L Q M G

V T P I I C T D E T M V Q K E V N G E I H Y V F Q Q L M S V

L R G V S L D Q I K N V V V S Y E P S W A V G Y G Q H A N P

V L A E E G C R Q I R R T I A D N Y T Y E I A D K I R I L Y

G G S V N P D N I G M I M N K P D V D G V L I G R A S L D V

D N F L R M V N Y L K N D Q E K

(30)M R K P F I I A N W K M N K N V H E S V A F V K A I K E K L

P A D K E I G I A A Q A V S L Y N M K K V A S S S N L Q I I

A Q N A S A E L E G P Y T G E I S M R S L A D A G V T Y V M

L G H L E R R R L F N E S N D S I N Q K V L A A L N A G I I

P I I C T D E E M V Q T E V N G Q I H Y V F R Q L K S V L K

G V P A N K L S Q I V I S Y E P S W A V G S T H Q A N P D I

A E E G C Q A I R Q S L V E M Y G N E I G E Q V R I L Y G G

S V N P E N I G Q I M S K P N V D G A L I G R A S L E I E S

F L Q M I N Y I E L A S K Q K L Q V I

(31)M R V P I I A G N W K M H K D V Q E A V S F I E K V K N Q L

P P A D Q L E T A I A A P T L C L V P M V K A A E E S P L K

I M A E N C Y Y K N E G A Y T G E T S P Y A L Y Q A G I H H

V I L G H S E R R T Y F N E T D E L I N K K V K A A L V N G

L C P I V C C D D T M R R R V A G K K V H W V V S R I L A D

L H G L T N D E I C H V T V A Y E P S W A I G T G E S A D P

E Q A A E G C Y L I R Q T I S D M Y G D E V A N N V R I L Y

G G S V T T S N I N A L M A K N D I D G V L V G A A S L N P

E T F L Q L V H H

24.根据权利要求13～23任一项所述的抗肥胖饮食品，其中，属于罗伊氏乳杆菌的微生物为罗伊氏乳杆菌JCM1112T。

25.一种甘油分解酶，其由下述(5)～(7)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示的亚基构成。

(5)M K R Q K R F E E L E K R P I H Q D T F V K E W P E E G F V

A M M G P N D P K P S V K V E N G K I V E M D G K K L E D F

D L I D L Y I A K Y G I N I D N V E K V M N M D S T K I A R

M L V D P N V S R D E I I E I T S A L T P A K A E E I I S K

L D F G E M I M A V K K M R P R R K P D N Q C H V T N T V D

N P V Q I A A D A A D A A L R G F P E Q E T T T A V A R Y A

P F N A I S I L I G A Q T G R P G V L T Q C S V E E A T E L

Q L G M R G F T A Y A E T I S V Y G T D R V F T D G D D T P

W S K G F L A S C Y A S R G L K M R F T S G A G S E V L M G

Y P E G K S M L Y L E A R C I L L T K A S G V Q G L Q N G A

V S C I E I P G A V P N G I R E V L G E N L L C M M C D I E

C A S G C D Q A Y S H S D M R R T E R F I G Q F I A G T D Y

I N S G Y S S T P N Y D N T F A G S N T D A M D Y D D M Y V

M E R D L G Q Y Y G I H P V K E E T I I K A R N K A A K A L

Q A V F E D L G L P K I T D E E V E A A T Y A N T H D D M P

K R D M V A D M K A A Q D M M D R G I T A I D I I K A L Y N

H G F K D V A E A I L N L Q K Q K V V G D Y L Q T S S I F D

K D W N V T S A V N D G N D Y Q G P G T G Y R L Y E D K E E

W D R I K D L P F A L D P E H L E L

(6)M A D I D E N L L R K I V K E V L S E T N Q I D T K I D F D

K S N D S T A T A T Q E V Q Q P N S K A V P E K K L D W F Q

P V G E A K P G Y S K D E V V I A V G P A F A T V L D K T E

T G I P H K E V L R Q V I A G I E E E G L K A R V V K V Y R

S S D V A F C A V Q G D H L S G S G I A I G I Q S K G T T V

I H Q K D Q D P L G N L E L F P Q A P V L T P E T Y R A I G

K N A A M Y A K G E S P E P V P A K N D Q L A R I H Y Q A I

S A I M H I R E T H Q V V V G K P E E E I K V T F D

(7)M S E V D D L V A K I M A Q M G N S S S A N S S T G T S T A

S T S K E M T A D D Y P L Y Q K H R D L V K T P K G H N L D

D I N L Q K V V N N Q V D P K E L R I T P E A L K L Q G E I

A A N A G R P A I Q K N L Q R A A E L T R V P D E R V L E M

Y D A L R P F R S T K Q E L L N I A K E L R D K Y D A N V C

A A W F E E A A D Y Y E S R K K L K G D N

26.核苷酸序列，其由下述(9)～(11)所表示，并编码权利要求25所述的甘油分解酶亚基。

(9)ATGAAACGTCAAAAACGATTTGAAGAACTAGAAAAACGGCCAATTCATCAAGATACATTT

GTTAAAGAATGGCCAGAAGAAGGTTTCGTTGCAATGATGGGGCCTAATGACCCTAAGCCT

AGTGTAAAAGTTGAAAATGGCAAGATCGTAGAGATGGATGGTAAAAAGCTCGAAGATTTT

GATTTGATTGACTTGTACATTGCTAAGTATGGAATCAATATTGACAACGTTGAAAAAGTT

ATGAATATGGATTCTACCAAGATTGCACGGATGCTTGTTGATCCTAATGTTTCTCGTGAT

GAAATTATTGAAATTACATCAGCTTTGACTCCTGCTAAGGCTGAAGAGATCATCAGTAAG

CTTGATTTTGGTGAAATGATTATGGCTGTCAAGAAGATGCGCCCACGTCGTAAGCCTGAC

AACCAGTGTCACGTTACCAATACTGTTGATAACCCAGTTCAAATTGCTGCTGATGCTGCT

GATGCCGCTCTTCGTGGATTTCCAGAACAAGAAACCACGACAGCTGTGGCACGTTATGCA

CCATTCAATGCTATTTCAATTTTAATTGGTGCACAAACAGGTCGCCCTGGTGTATTGACA

CAATGTTCTGTTGAAGAAGCTACTGAATTGCAATTAGGTATGCGTGGTTTTACCGCATAT

GCTGAAACCATTTCAGTTTACGGTACTGATCGTGTATTTACCGATGGTGATGATACTCCA

TGGTCTAAAGGCTTCTTGGCATCTTGTTATGCATCACGTGGTTTGAAGATGCGATTTACT

TCAGGTGCCGGTTCAGAAGTTTTGATGGGTTATCCAGAAGGTAAGTCAATGCTTTACCTT

GAAGCGCGTTGTATTTTACTTACTAAGGCTTCAGGTGTTCAAGGACTTCAAAATGGTGCC

GTAAGTTGTATTGAAATTCCTGGTGCTGTTCCTAATGGTATTCGTGAAGTTCTCGGTGAA

AACTTGTTATGTATGATGTGTGACATCGAATGTGCTTCTGGTTGTGACCAAGCATACTCA

CACTCCGATATGCGGCGGACTGAACGGTTTATTGGTCAATTTATTGCCGGTACTGATTAT

ATTAACTCTGGTTACTCATCAACTCCTAACTACGATAATACCTTCGCTGGTTCAAACACT

GATGCTATGGACTACGATGATATGTATGTTATGGAACGTGACTTGGGTCAATATTATGGT

ATTCACCCTGTTAAGGAAGAAACCATTATTAAGGCACGTAATAAGGCCGCTAAAGCCCTT

CAAGCAGTATTTGAAGATCTTGGATTACCAAAGATTACTGATGAAGAGGTCGAAGCAGCA

ACGTATGCTAACACCCATGATGACATGCCAAAGCGGGATATGGTTGCAGATATGAAGGCT

GCTCAAGATATGATGGATCGTGGAATTACTGCTATTGATATTATCAAGGCATTGTACAAC

CACGGATTTAAAGATGTCGCTGAAGCAATTTTGAACCTTCAAAAACAAAAAGTTGTTGGT

GATTACCTTCAAACATCTTCTATTTTTGATAAAGATTGGAACGTCACTTCTGCTGTTAAC

GACGGAAATGATTATCAAGGACCAGGTACTGGATACCGTCTATATGAAGACAAGGAAGAA

TGGGATCGGATTAAAGACTTACCATTCGCCCTTGATCCAGAACATTTGGAACTGTAG

(10)ATGGCTGATATTGATGAAAACTTATTACGTAAAATCGTTAAAGAAGTTTTAAGCGAAACT

AATCAAATCGATACTAAGATTGACTTTGATAAAAGTAATGATAGTACTGCAACAGCAACT

CAAGAGGTGCAACAACCAAATAGTAAAGCTGTTCCAGAAAAGAAACTTGACTGGTTCCAA

CCAGTTGGAGAAGCAAAACCTGGATATTCTAAGGATGAAGTTGTAATTGCAGTCGGTCCT

GCATTCGCAACTGTTCTTGATAAGACAGAAACTGGTATTCCTCATAAAGAAGTGCTTCGT

CAAGTTATTGCTGGTATTGAAGAAGAAGGGCTTAAGGCGCGGGTAGTTAAAGTTTACCGG

AGTTCAGATGTAGCATTCTGTGCTGTCCAAGGTGATCACCTTTCTGGTTCAGGAATTGCT

ATTGGTATCCAATCAAAAGGGACGACAGTTATTCACCAAAAGGATCAAGACCCTCTTGGT

AACCTTGAGTTATTCCCACAAGCGCCAGTACTTACTCCCGAAACTTATCGTGCAATTGGT

AAGAATGCCGCTATGTATGCTAAGGGTGAATCTCCAGAACCAGTTCCAGCTAAAAACGAT

CAACTTGCTCGTATTCACTATCAAGCTATTTCAGCAATTATGCATATTCGTGAAACTCAC

CAAGTTGTTGTTGGTAAGCCTGAAGAAGAAATTAAGGTTACGTTTGATTAA

(11)ATGAGTGAAGTTGATGATTTAGTAGCAAAGATCATGGCTCAGATGGGAAACAGTTCATCT

GCTAATAGCTCTACAGGTACTTCAACTGCAAGTACTAGTAAGGAAATGACAGCAGATGAT

TACCCACTTTATCAAAAGCACCGTGATTTAGTAAAAACACCAAAAGGACACAATCTTGAT

GACATCAATTTACAAAAAGTAGTAAATAATCAAGTTGATCCTAAGGAATTACGGATTACA

CCAGAAGCATTGAAACTTCAAGGTGAAATTGCAGCTAATGCTGGCCGTCCAGCTATTCAA

AAGAATCTTCAACGAGCTGCAGAATTAACACGAGTACCTGACGAACGGGTTCTTGAAATG

TATGATGCATTGCGTCCTTTCCGTTCAACTAAGCAAGAATTATTGAACATTGCAAAGGAA

TTACGGGACAAGTATGACGCTAATGTTTGCGCAGCATGGTTTGAAGAAGCTGCTGATTAT

TATGAAAGTCGTAAGAAGCTAAAGGGCGATAACTAA

27.肠道黏附性蛋白质，其由下述(8)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示。

(8)M F G H D G R I V T K V Y Q W A G T Y Y Y F D P N T Y L R V

D N D Y R Q S Q W G D W Y M F G P D G R I V T G L K E W Y G

S Y Y Y F D P T T Y L K V T N K W I D N K Y F G P A G Q Q A

I S R F E R L D N K Y Y Y F D A N G A V L N I H D Q F K N I

D N H T Y Y F G A D G A C Y T S Q F L N K D G K Q Y Y F D N

D G I M L T D Q E K I I D G K F Y H F N V N G E A I Q V N D

P S E I

28.核苷酸序列，其由下述(12)所表示，并编码权利要求27所述的肠道黏附性蛋白质。

(12)ATGTTCGGTCACGATGGCCGCATTGTTACTAAAGTTTACCAATGGGCTGGCACGTATTAC

TACTTTGATCCGAATACTTATTTGCGAGTAGATAATGATTACCGTCAATCTCAGTGGGGC

GATTGGTATATGTTTGGCCCAGATGGTCGTATCGTTACAGGGTTAAAGGAATGGTACGGT

AGTTATTATTACTTTGATCCGACGACTTACTTAAAAGTAACTAATAAGTGGATAGATAAT

AAGTACTTTGGTCCAGCTGGTCAGCAAGCTATTTCACGCTTTGAGAGACTTGATAATAAG

TATTACTATTTCGATGCTAATGGGGCAGTTCTTAATATCCATGATCAATTTAAGAATATT

GATAACCACACTTATTACTTTGGAGCTGATGGTGCTTGTTATACCAGTCAATTCTTAAAT

AAGGATGGTAAACAGTATTATTTCGATAATGATGGAATTATGCTCACTGATCAAGAGAAG

ATCATTGACGGTAAATTCTATCATTTCAATGTTAATGGTGAAGCAATCCAAGTAAATGAT

CCTTCTGAAATTTGA

29.甘油分解酶，其由下述(16)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示。

(16)M K A A V I N D P V D G F V T V K D V Q L R D L K P G E A L

V D M E Y C G L C H T D L H V A A G D F G K K P G R I I G H

E G V G R V S K V A P G V T S L K V G D R V S I A W F F K G

C G H C E Y C L T G R E T L C R N V L N A G Y T A D G A M A

E Q C I V P A D Y A V K V P E G L D P V E A T S L T C A G V

T M Y K A L K V A D I K P G Q W V S I V G A G G L G N L G I

Q L A H N V F G A H V I A V D G N P D K L E A A K K N G A E

I L I N R H D G D V D K Q I Q E K V G G V H A A V V T A V S

A S A F D Q A V D S L R P D G K L V A V A L P Q G D M K L N

I A K T V L D G I I V A G S L V G T R Q D L A E C F Q F G A

E G K V H P I V K T R K L S E I N D M I Q E L K D N K V V G

R N V V D F V H N D N D

30.核苷酸序列，其由下述(32)所表示，并编码权利要求29所述的甘油分解酶。

(32)ATGAAAGCTGCTGTTATTAATGATCCAGTAGACGGTTTTGTTACTGTTAAAGATGTTCAA

CTTCGGGATTTGAAGCCCGGTGAAGCTTTAGTTGACATGGAATATTGTGGTCTTTGTCAC

ACTGATCTACACGTTGCTGCTGGGGACTTTGGTAAGAAGCCCGGTCGTATTATCGGTCAC

GAAGGGGTTGGTCGTGTATCTAAGGTTGCCCCTGGCGTTACTTCCTTGAAAGTTGGCGAC

CGTGTATCAATTGCATGGTTCTTCAAGGGCTGTGGACACTGTGAATATTGTTTAACTGGT

CGTGAAACTCTTTGTCGGAACGTTCTTAATGCGGGTTACACTGCTGACGGTGCAATGGCT

GAACAATGTATCGTACCAGCTGACTACGCTGTTAAGGTTCCAGAAGGTCTTGATCCTGTT

GAAGCTACTTCATTAACTTGTGCTGGTGTTACGATGTACAAGGCATTAAAGGTTGCTGAC

ATCAAGCCAGGTCAATGGGTATCAATCGTTGGTGCTGGTGGTTTAGGTAACTTGGGTATT

CAACTTGCTCACAACGTATTTGGTGCTCATGTTATCGCTGTTGATGGTAATCCTGATAAG

CTTGAAGCCGCTAAGAAGAATGGTGCTGAAATTTTAATTAACCGTCATGACGGTGATGTT

GATAAGCAAATTCAAGAAAAGGTTGGCGGTGTTCACGCTGCTGTAGTAACAGCTGTTTCT

GCCTCTGCATTCGACCAAGCAGTTGATTCACTTCGCCCAGATGGTAAGCTTGTTGCCGTT

GCGCTTCCACAAGGTGACATGAAGCTTAACATTGCTAAGACTGTTCTTGATGGTATCATT

GTTGCTGGTTCATTAGTTGGTACCCGTCAAGACTTAGCTGAATGTTTCCAATTTGGTGCA

GAAGGTAAGGTTCACCCAATTGTTAAGACTCGTAAGTTAAGCGAAATTAATGATATGATC

CAAGAACTTAAGGATAACAAGGTTGTTGGTCGGAATGTTGTTGATTTTGTTCACAACGAT

AACGACTAA

31.甘油分解酶，其由下述(17)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示。

(17)M E K R E N A I P K T M K A W A V T T P G P I D G K E S P I

E F T E K P V P T P K R G E V L V K V I T C G V C H T D L H

V T E G D L P V H H E H V T P G H E I V G K V V G F G P E T

Q R F K F G E R I G I P W F R H A C G V C K F C R S G H E N

L C P H S L Y T G W D H D G G Y A E Y V T V P E G F A Y R L

P E K F D S L E A A P L L C A G I I G Y R A F E R A N V P A

G G R L G L Y G F G G S A H I T A Q I A L A Q G I E V H V F

T R G E D A K K F A L E L G C A S V Q G S Y D P A P V P L D

S S I I F A P V G D M V L P A L A S L V P G G T L A L A G I

H M T D I P T M N Y Q K E I F H E K T L T S V E S N T R R D

G E E F L T L A D R L N I H P E V H E Y P L A K A D E A L R

Y V K H G D I K G A C V L R V S E D

32.核苷酸序列，其由下述(33)所表示，并编码权利要求31所述的甘油分解酶。

(33)ATGGAAAAACGCGAAAATGCTATTCCGAAAACAATGAAGGCTTGGGCAGTCACAACTCCT

GGGCCGATTGATGGTAAGGAATCACCAATCGAATTTACCGAAAAGCCTGTGCCGACTCCT

AAACGGGGAGAAGTCCTTGTTAAGGTAATAACGTGTGGAGTATGTCATACGGACTTGCAC

GTGACTGAAGGAGACTTGCCGGTTCACCACGAACACGTTACTCCTGGTCATGAAATTGTT

GGTAAAGTTGTCGGCTTTGGACCAGAGACACAACGATTTAAGTTTGGTGAGCGAATTGGG

ATTCCATGGTTTCGGCATGCTTGTGGTGTATGCAAGTTTTGCCGATCAGGTCATGAGAAT

CTCTGTCCTCATTCACTTTATACCGGTTGGGATCATGATGGCGGTTATGCAGAATATGTC

ACAGTTCCAGAAGGATTTGCATATCGGCTTCCAGAAAAGTTTGATTCCCTAGAGGCAGCT

CCGTTATTATGTGCAGGGATTATTGGTTATCGGGCCTTTGAACGTGCCAATGTTCCGGCT

GGCGGTCGCCTAGGATTATATGGCTTCGGTGGTTCAGCTCATATTACAGCTCAAATTGCA

CTTGCTCAGGGAATTGAAGTGCATGTCTTTACGCGTGGTGAGGATGCCAAGAAATTCGCC

CTAGAATTAGGTTGTGCTTCTGTTCAGGGCTCCTATGACCCAGCACCAGTTCCTTTGGAT

TCATCAATCATTTTTGCGCCGGTTGGTGATATGGTCTTGCCGGCTTTAGCTAGTTTAGTT

CCAGGGGGGACATTAGCATTAGCCGGTATTCATATGACTGATATTCCAACAATGAATTAC

CAAAAAGAAATATTCCACGAAAAGACATTAACGAGTGTTGAGAGTAATACTCGTCGTGAT

GGGGAAGAATTCTTAACATTAGCTGATCGTCTTAATATCCATCCTGAAGTCCACGAATAT

CCCCTAGCAAAGGCTGACGAAGCATTACGCTATGTTAAGCACGGTGATATTAAGGGAGCT

TGTGTATTACGTGTTAGTGAGGACTAA

33.甘油分解酶，其由下述(18)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示。

(18)M Q I K A A L A T K P N A D L E I Q T V E L D E P K E N E V

L I K I A S T G F C H T D I V G R S G A T T P L P V V L G H

E G A G V V Q K V G A N V T D V K P G D H V V L S F S Y C G

H C Y N C T H N H Q G L C E N F N Q L N F E G K T Y D G T H

R L H L D D G T P V S V F F G Q S S F A T Y V T A N V H N I

V K V D Q D V D L N L L G P L G C G M Q T G A G T V L N Y I

K P A P E D A I A V F G A G A V G L A A I M A A K I A G V K

H I I A I N R N G N H L D L A K E L G A T E T I N N T A E D

P V K A I K E I V P R G V T Y A I D T T G N T G V I K S A I

D S L A T A G E C V L L G V G G D I T L D L M N D I L S E S

K K I S G V V E G D S N P Q E F I P Q L V K Y Y K Q S K F P

L D K L V K Y Y D F A D I N Q V I A D S T N G K V I K P I I

K I D P E L A K L P L T N D G S N V Q K M V A E A G L A D Q

I T I D S A G T S N I A E G S P A D S R T K A I L D K Y H I

K D D G M I A R Q L Q D R D Y Y D A D Y I I A M D Q M N V R

D A K D M A P A G L E N K V H G I F E A T P G K E N C Y I V

D P W I T H

34.核苷酸序列，其由下述(34)所表示，并编码权利要求33所述的甘油分解酶。

(34)ATGCAAATTAAAGCTGCTCTTGCAACCAAACCTAACGCTGATTTAGAGATTCAAACCGTC

GAATTGGATGAACCAAAAGAAAATGAAGTATTAATAAAAATTGCTTCAACAGGTTTTTGT

CATACAGATATTGTTGGTCGAAGCGGTGCCACTACCCCTCTCCCCGTTGTCCTCGGGCAT

GAAGGTGCGGGCGTCGTCCAAAAAGTAGGAGCTAACGTTACGGACGTTAAACCCGGCGAC

CATGTTGTTCTATCATTTAGCTACTGTGGCCATTGCTATAACTGTACTCATAATCATCAA

GGCTTATGCGAAAACTTCAATCAGCTAAACTTTGAAGGAAAAACCTATGATGGTACTCAC

CGCCTGCACTTAGATGATGGCACGCCAGTCAGTGTCTTTTTTGGTCAGTCTTCCTTTGCG

ACCTATGTAACAGCCAATGTCCATAATATTGTTAAAGTTGATCAAGATGTTGATCTTAAC

TTATTAGGGCCACTCGGTTGTGGAATGCAAACAGGTGCTGGAACCGTTCTAAATTATATT

AAACCTGCTCCTGAAGATGCAATTGCCGTTTTCGGTGCTGGTGCTGTTGGCTTAGCCGCA

ATTATGGCTGCTAAAATTGCTGGAGTTAAACATATTATTGCGATTAATCGTAACGGTAAC

CACCTTGACCTGGCGAAGGAATTGGGCGCTACTGAAACGATTAATAATACGGCTGAAGAT

CCCGTCAAAGCAATTAAAGAAATCGTTCCGCGTGGTGTAACTTATGCAATCGATACTACC

GGAAACACCGGTGTAATTAAATCAGCAATTGATAGTCTTGCCACCGCTGGAGAATGTGTC

CTCTTAGGAGTTGGCGGCGATATTACCTTAGACTTAATGAATGATATCTTATCAGAATCT

AAGAAAATCTCTGGGGTTGTCGAAGGAGATAGCAATCCCCAAGAGTTTATTCCTCAACTA

GTTAAGTACTACAAGCAAAGCAAGTTCCCCCTTGATAAGCTTGTTAAGTACTACGATTTT

GCTGATATTAACCAAGTTATCGCTGACTCAACAAACGGAAAGGTTATTAAGCCAATCATC

AAAATTGATCCTGAATTAGCTAAATAATTGCCGCTCACCAATGACGGAAGCAATGTTCAA

AAAATGGTTGCAGAAGCTGGCCTTGCTGATCAAATTACTATTGATTCAGCCGGAACAAGT

AACATTGCAGAAGGTTCACCTGCTGATAGTCGAACAAAAGCCATTCTCGATAAATATCAC

ATTAAAGACGACGGAATGATTGCCCGTCAATTGCAGGACAGGGATTATTATGATGCCGAT

TATATTATCGCAATGGATCAGATGAATGTCCGGGACGCAAAAGATATGGCACCAGCTGGG

TTAGAAAATAAGGTTCATGGAATCTTTGAAGCTACCCCAGGAAAAGAAAATTGCTATATC

GTTGACCCCTGGATCACTCACTGA

35.甘油分解酶，其由下述(19)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示。

(19)M K K A I F E K A G Q M K I V D V D R P T I E K P D D V I I

K V V R T C V C G S D L W N F R G I N P V E K D S E N S G H

E A I G I V E E V G E D I T T V K P G D F V I A P F T H G C

G H C A A C R A G F D G S C Q S H N D N F S S G V Q A Q Y V

R F Q H G Q W A L V K V P G K P S D Y S E G M L K S L L T L

A D V M A T G Y H A A R V A N V S D G D T V V V M G D G A V

G L C A I I A A K M R G A K K I I S T S R H A D R Q A L A K

E F G A T D N V A E R S D E A V Q K I M E L T N G A G A D A

V L E C V G T E Q S T D T A M K V G R P G T I V G R V G L P

H T P K M D M T V L F Y N N T I V G G G P A S V T T Y D K D

V L L K A V L D G D I N P G K V F T K S F D L D Q I Q E A Y

E A M D K R E A I K S Y I I M D G F E R D

36.核苷酸序列，其由下述(35)所表示，并编码权利要求35所述的甘油分解酶。

(35)ATGAAAAAAGCTATTTTTGAAAAGGCGGGTCAAATGAAGATTGTTGATGTTGACCGTCCA

ACAATTGAAAAGCCTGATGACGTAATTATTAAGGTAGTGCGGACCTGTGTTTGTGGTTCT

GACCTATGGAACTTCCGAGGAATTAATCCGGTTGAAAAAGATTCTGAAAACTCTGGCCAT

GAAGCAATTGGAATTGTTGAAGAAGTTGGTGAAGATATCACTACTGTCAAACCTGGGGAC

TTTGTGATTGCTCCATTTACTCATGGATGTGGGCACTGTGCTGCTTGTCGCGCGGGCTTC

GATGGTTCTTGCCAAAGTCACAACGATAACTTTAGCTCTGGTGTGCAAGCTCAATACGTT

CGGTTCCAACACGGTCAATGGGCGCTTGTTAAAGTTCCGGGCAAGCCAAGTGACTACAGT

GAAGGAATGCTTAAGTCCCTCTTAACCCTTGCTGATGTTATGGCTACTGGTTACCACGCT

GCACGAGTTGCTAACGTTAGTGATGGTGATACAGTTGTTGTAATGGGTGACGGTGCTGTT

GGCCTTTGTGCGATTATTGCTGCTAAGATGCGGGGCGCTAAGAAGATCATTTCTACTAGT

CGCCATGCTGACCGTCAAGCCCTTGCTAAGGAATTTGGTGCTACTGACAATGTTGCTGAA

CGTAGTGACGAAGCGGTTCAAAAGATCATGGAACTCACTAACGGTGCCGGTGCTGATGCT

GTCCTTGAATGCGTTGGTACTGAACAATCAACTGATACTGCCATGAAAGTTGGCCGTCCA

GGTACCATCGTTGGTCGGGTTGGCTTACCTCATACCCCAAAGATGGACATGACGGTGCTA

TTCTACAACAACACTATTGTCGGCGGTGGTCCAGCATCAGTAACCACTTACGACAAGGAC

GTATTGTTGAAGGCTGTTCTTGATGGTGACATTAACCCTGGTAAGGTCTTTACTAAGAGC

TTCGACCTTGACCAAATTCAAGAAGCTTATGAAGCAATGGATAAGCGTGAAGCAATCAAG

TCTTACATTATTATGGATGGCTTTGAACGCGATTAA

37.甘油分解酶，其由下述(20)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示。

(20)M G R L D N K V A I I T G G S K G I G A A V A K K F I E E G

A K V V L T A R K M D E G Q K V A D Q L G D N A I F I Q Q D

V A R K G D W D R V I R Q T V Q V F G K L N I V V N N A G I

A E Y A D V E K T D A E I W D K T I A V N L T G T M W G T K

L G I E A M K N N G E K N S I I N M S S I E G L I G D P D L

F A Y N A S K G G V R L L T K S A A L D C A R K G Y D I R V

N T I H P G Y I S T P L V D N L V K D D P K A E G H L E S L

H P L G R L G K P E E I A N L A L Y L A S D E S S F S T G S

E F V A D G G Y T A Q

38.核苷酸序列，其由下述(36)所表示，并编码权利要求37所述的甘油分解酶。

(36)ATGGGTCGTTTAGATAATAAAGTTGCAATTATTACTGGTGGTTCTAAAGGAATTGGAGCT

GCTGTCGCAAAAAAGTTTATCGAAGAAGGCGCAAAGGTTGTTTTAACCGCTCGGAAGATG

GATGAGGGACAAAAAGTCGCTGACCAACTAGGTGACAATGCGATCTTTATCCAACAAGAC

GTTGCTCGGAAAGGAGACTGGGACCGGGTAATCCGCCAAACTGTCCAAGTCTTTGGGAAG

CTCAATATTGTGGTTAACAATGCGGGAATTGCCGAATACGCCGATGTTGAGAAGACGGAC

GCTGAAATTTGGGATAAAACAATTGCCGTTAACCTTACCGGTACGATGTGGGGAACTAAG

CTCGGTATTGAAGCAATGAAGAACAACGGGGAAAAGAATTCAATCATCAATATGTCATCC

ATTGAAGGACTAATTGGTGATCCTGATCTCTTTGCATACAATGCTTCTAAGGGTGGTGTC

CGCCTCTTAACTAAGTCCGCTGCGCTTGATTGTGCCCGGAAAGGCTATGACATCCGTGTA

AATACAATTCATCCTGGTTATATCTCAACTCCACTAGTTGATAATTTGGTCAAGGATGAT

CCAAAAGCAGAAGGACACCTAGAAAGCCTTCATCCCCTTGGCCGTCTTGGAAAGCCAGAA

GAGATTGCTAACCTCGCTTTATACCTTGCTTCAGATGAATCAAGCTTTAGTACTGGTTCG

GAATTTGTCGCTGATGGTGGCTATACGGCTCAATAA

39.甘油分解酶，其由下述(21)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示。

(21)M T N V P T V K L N N G V E M P T L G F E V F Q V P D L S Q

A E Q A V T D A L E V G Y R L I D T A A A Y Q N E E A V G K

A I K N S S V N R E D V F V T S K L W V S D F N Y K R A K A

G I D A S L Q K L G L D Y M D L Y L L H Q P Y G D T M G A W

R A L Q E A Q K E G K I R A I G V S N F Y A D Q L K D L E L

T M P V K P A V N Q I E V N P W Y Q Q D Q E V K F A Q S E D

I R V E A W A P F A E G K H D I F T N E I I A E I A A K Y G

K S N G Q V I L R W L L Q R G I T V I P K S V H K N R M E E

N I D V F D F E L S N D D M K K I A S L N K K E S Q F F D H

R D P V T I E Q I F G S S L K M V Q D D E K

40.核苷酸序列，其由下述(37)所表示，并编码权利要求39所述的甘油分解酶。

(37)ATGACAAATGTACCAACAGTAAAATTAAATAACGGAGTAGAAATGCCAACCCTTGGATTT

GAAGTATTCCAAGTTCCAGACTTAAGCCAAGCTGAACAAGCAGTTACCGATGCTCTTGAA

GTCGGCTATCGTTTAATCGATACTGCTGCTGCTTACCAAAATGAAGAAGCAGTTGGAAAG

GCAATTAAGAATAGTAGTGTAAACCGTGAAGATGTCTTTGTAACTTCTAAGTTATGGGTG

TCTGATTTTAACTATAAGCGGGCTAAAGCAGGGATTGACGCTTCACTGCAAAAACTTGGC

CTTGATTACATGGATCTTTACCTTCTCCATCAACCATATGGCGATACAATGGGGGCTTGG

CGAGCATTACAAGAAGCACAGAAAGAAGGTAAGATTCGCGCAATCGGTGTATCGAACTTC

TACGCTGATCAACTAAAGGATCTTGAATTAACAATGCCTGTTAAGCCAGCGGTCAACCAA

ATTGAAGTTAACCCTTGGTACCAGCAAGATCAAGAGGTTAAGTTTGCGCAAAGTGAAGAT

ATTCGTGTTGAAGCATGGGCACCATTTGCGGAAGGTAAGCATGATATTTTTACCAACGAA

ATAATTGCGGAAATTGCTGCCAAGTATGGCAAGAGCAATGGTCAAGTAATTCTTCGCTGG

CTTTTACAACGGGGTATTACTGTCATTCCAAAGTCAGTCCACAAGAACCGGATGGAAGAA

AATATCGATGTCTTTGATTTTGAACTTTCCAATGATGATATGAAAAAGATAGCTAGTCTT

AACAAGAAGGAAAGCCAATTCTTTGACCACCGTGATCCGGTTACGATTGAACAAATCTTT

GGCTCCAGCTTAAAGATGGTTCAAGATGACGAAAAATAA

41.甘油分解酶，其由下述(22)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示。

(22)M I L D E T I T L N S G V K I P K F A L G T W M I D D D Q A

A E A V R N A I K M G Y R H I D T A Q A Y D N E R G V G E G

V R T A G I D R D K I F V T S K I A A E H K D Y D V T K K S

I D E T L E K M G L D Y I D M M L I H S P Q P W K E V N Q S

D N R Y L E G N L A A W R A M E D A V N E G K I R T I G V S

N F K K A D L E N I I K N S D T V P A V D Q V L A H I G H T

P F N L L S F T H E H D I A V E A Y S P V A H G A A L D N P

V I E K M A K K Y N V S V P Q L C I R Y D W Q I G M I V L P

K T T N P E H M K E N T E I D F E I S E A D M D L L R R V K

P L D Y G D F D I Y P V Y G G K M

42.核苷酸序列，其由下述(38)所表示，并编码权利要求41所述的甘油分解酶。

(38)ATGATTTTAGATGAGACAATTACTCTTAATAGTGGTGTGAAAATTCCAAAGTTTGCATTA

GGAACCTGGATGATTGATGATGACCAAGCAGCCGAAGCAGTTCGGAATGCGATTAAGATG

GGATATCGGCACATCGATACAGCTCAGGCTTATGATAATGAGCGGGGAGTCGGTGAAGGT

GTACGAACAGCCGGTATTGATCGGGATAAAATCTTTGTTACTTCAAAGATCGCTGCTGAA

CACAAAGATTATGATGTAACTAAAAAGTCGATTGACGAGACTCTTGAAAAGATGGGTCTT

GATTATATCGACATGATGCTTATTCATAGTCCTCAACCATGGAAAGAAGTAAATCAATCT

GATAATCGTTACCTTGAAGGAAATCTCGCTGCTTGGCGAGCCATGGAAGATGCCGTTAAC

GAAGGTAAGATTCGAACAATTGGCGTTTCTAATTTCAAAAAAGCCGATCTTGAAAATATT

ATTAAGAATAGCGATACCGTTCCCGCTGTTGATCAAGTTTTAGCTCATATTGGTCATACT

CCATTCAATCTTTTATCATTTACTCATGAACATGACATTGCGGTTGAAGCATATTCACCA

GTTGCTCACGGCGCTGCTTTAGACAACCCCGTAATTGAAAAGATGGCTAAAAAGTACAAC

GTTTCAGTCCCACAATTGTGCATTCGGTATGATTGGCAAATAGGAATGATCGTCTTACCA

AAGACTACTAATCCAGAACACATGAAGGAAAACACTGAAATTGATTTTGAAATTTCTGAA

GCTGATATGGACCTATTGCGGCGAGTAAAGCCATTAGACTATGGCGATTTTGATATCTAC

CCTGTTTACGGTGGAAAAATGTAA

43.醛脱氢酶，其由下述(23)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示。

(23)M P A N N K K Q V E K K E L T A E E K K Q N A Q K L V D D L

M T K S Q A A F E K L R Y Y S Q E Q V D K I C Q A M A L A A

E E H H M D L A V D A A N E T G R G V A E D K A I K N I Y A

S E Y I W N N I R H D K T V G I I E D N D E D Q T I K I A D

P L G V I A G I V P V T N P T S T T I F K S I I S A K T R N

T I I F S F H R Q A M K S S I K T A K I L Q E A A E K A G A

P K N M I Q W L P E S T R E N T T A L L Q H P N T A T I L A

T G G P S L V K A A Y S S G N P A L G V G P G N G P A Y I E

K T A N I E R S V Y D I V L S K T F D N G M I C A T E N S V

V V D E E I Y D K V K E E F Q K W N C Y F L K P N E I D K F

T D G F I D P D R H Q V R G P I A G R S A N A I A D M C G I

K V P D N T K V I I A E Y E G V G D K Y P L S A E K L S P V

L T M Y K A T S H E N A F D I C A Q L L H Y G G E G H T A A

I H T L D D D L A T K Y G L E M R A S R I I V N S P S G I G

G I G N I Y N N M T P S L T L G T G S Y G S N S I S H N V T

D W D L L N I K T I A K R R E N R Q W V K I P P K V Y F Q R

N S L K E L Q D I P N I N R A F I V T G P G M S K R G Y V Q

R V I D Q L R Q R Q N N T A F L V F D D V E E D P S T N T V

E K G V A M M N D F K P D T I I A L G G G S P M D A A K A M

W M F Y E H P E T S W Y G V M Q K Y L D I R K R A Y Q I K K

P T K S Q L I G I P T T S G T G S E V T P F A V I T D S K T

H V K Y P L A D Y A L T P N I A I V D S Q F V E T V P A K T

T A W T G L D V L C H A T E S Y V S V M A T D Y T R G W S L

Q T I K G V M E N L P K S V Q G D K L A R R K M H D F S T M

A G M A F G Q A F L G I N H S L A H K M G G A F G L P H G L

L I A I A M P Q V I R F N A K R P Q K L A L W P H Y E T Y H

A T K D Y A D I A R F I G L K G N T D E E L A E A Y A K K V

I E L A H E C G V K L S L K D N G V T R E E F D K A V D D L

A R L A Y E D Q C T T T N P V E P L V S Q L K E L L E R C Y

D G T G V E E K

44.核苷酸序列，其由下述(39)所表示，并编码权利要求43所述的醛脱氢酶。

(39)ATGCCTGCTAACAACAAGAAACAAGTTGAAAAGAAAGAATTAACTGCTGAAGAAAAAAAG

CAAAACGCCCAAAAGCTAGTTGACGATTTAATGACTAAGAGTCAAGCTGCTTTTGAAAAG

TTACGTTACTATTCACAAGAACAAGTTGACAAGATTTGTCAGGCAATGGCTCTCGCTGCC

GAAGAACACCACATGGACTTAGCTGTTGATGCTGCTAACGAAACTGGTCGTGGGGTTGCT

GAAGATAAGGCTATCAAGAACATCTACGCAAGTGAATACATTTGGAACAACATCCGTCAC

GATAAGACTGTTGGTATTATCGAAGACAATGATGAAGACCAAACTATCAAAATTGCTGAT

CCACTTGGTGTCATTGCCGGAATTGTTCCAGTTACTAACCCTACTTCAACAACGATCTTC

AAATCAATCATTAGTGCTAAGACACGGAATACAATCATCTTTTCTTTCCACCGTCAAGCA

ATGAAGTCATCTATCAAGACTGCAAAGATTCTCCAAGAAGCTGCTGAAAAAGCCGGTGCG

CCAAAGAACATGATTCAATGGCTCCCTGAAAGTACCCGCGAAAACACTACCGCATTACTC

CAACACCCTAATACTGCTACTATTTTAGCAACCGGTGGTCCTTCATTAGTTAAGGCTGCC

TACAGTTCTGGTAACCCTGCTCTTGGTGTTGGTCCTGGTAACGGTCCTGCTTACATCGAA

AAAACTGCCAACATCGAACGTTCTGTTTACGACATCGTTCTTTCTAAGACATTCGATAAC

GGTATGATTTGTGCCACTGAAAACTCAGTTGTTGTTGATGAAGAAATCTACGACAAGGTT

AAAGAAGAATTCCAAAAGTGGAACTGTTACTTCTTGAAGCCAAACGAAATTGATAAATTT

ACTGATGGCTTTATTGACCCAGATCGTCATCAAGTTCGTGGTCCAATCGCTGGTCGTTCA

GCTAATGCTATTGCTGACATGTGTGGTATTAAAGTACCTGACAACACTAAGGTTATCATT

GCTGAATACGAAGGTGTTGGTGACAAGTACCCACTTTCAGCTGAAAAGCTTTCACCAGTA

TTAACAATGTACAAGGCAACCTCTCACGAAAATGCCTTTGATATCTGTGCTCAATTATTA

CACTACGGTGGTGAAGGTCACACTGCTGCTATTCACACCCTTGATGATGATTTAGCTACT

AAGTACGGTCTTGAAATGCGTGCTTCACGGATCATTGTTAACTCCCCATCTGGTATTGGT

GGTATTGGTAACATCTACAACAACATGACTCCATCCCTTACTTTAGGTACTGGTTCATAC

GGTAGTAACTCAATTTCTCACAACGTTACTGATTGGGACCTCTTAAACATCAAAACAATT

GCAAAGCGGCGTGAAAACCGTCAATGGGTTAAGATTCCCCCAAAAGTATACTTTCAACGC

AACTCACTAAAAGAATTGCAAGATATTCCAAACATTAACCGGGCATTCATCGTTACTGGT

CCTGGAATGAGCAAGCGTGGTTACGTTCAACGTGTTATCGATCAATTGCGTCAACGCCAA

AACAACACTGCTTTCTTAGTATTTGATGACGTTGAAGAAGATCCATCAACAAACACTGTT

GAAAAAGGTGTTGCCATGATGAATGACTTCAAACCTGATACAATTATTGCTCTTGGTGGT

GGTTCACCAATGGATGCTGCTAAGGCTATGTGGATGTTCTATGAGCACCCAGAAACTTCA

TGGTATGGGGTTATGCAAAAGTACCTTGATATTCGGAAGCGTGCTTACCAAATCAAGAAG

CCTACTAAGTCTCAACTTATTGGTATCCCTACTACATCAGGTACTGGTTCAGAAGTTACT

CCATTTGCGGTTATTACCGATTCAAAAACTCATGTTAAGTACCCACTTGCTGACTACGCC

TTAACACCAAACATTGCAATCGTTGACTCACAATTCGTTGAAACTGTTCCAGCAAAAACT

ACTGCTTGGACTGGACTAGATGTTTTATGTCACGCTACTGAATCATATGTTTCTGTTATG

GCAACTGACTACACTCGTGGTTGGTCACTACAAACCATCAAGGGTGTTATGGAAAACCTT

CCTAAGTCAGTTCAAGGTGATAAGTTAGCTCGTCGTAAGATGCACGACTTCTCAACAATG

GCCGGGATGGCATTTGGTCAAGCCTTCTTAGGAATTAACCACTCCCTTGCCCACAAGATG

GGTGGAGCATTCGGTCTTCCTCACGGTTTGCTTATCGCTATTGCAATGCCACAAGTAATT

CGCTTTAACGCAAAACGTCCACAAAAGCTTGCTCTCTGGCCTCACTATGAGACTTACCAT

GCAACTAAGGACTACGCTGACATTGCACGGTTCATTGGTTTGAAAGGCAACACTGATGAA

GAATTAGCTGAAGCATATGCTAAGAAAGTTATCGAACTTGCTCACGAATGTGGTGTTAAG

CTTAGTCTTAAGGACAATGGTGTTACACGTGAAGAATTTGATAAGGCGGTTGACGATCTT

GCTCGCTTAGCTTACGAAGATCAATGTACTACTACTAACCCAGTTGAACCACTTGTTAGC

CAACTCAAGGAATTACTTGAACGTTGCTACGATGGTACTGGCGTTGAAGAAAAATAA

45.醛脱氢酶，其由下述(24)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示。

(24)M A Y Q S I N P F T N Q V E K T F E N T T D E E L E Q T L T

T A H Q L Y L D W R K Y N D L E E R K R Q I L K L G Q I L R

E R R V E Y A T V M S K E M G K L I S E A E G E V D L C A S

F C D Y Y A A H A D E F L Q P K I I A T T S G R A K V L K Q

S L G I L V A V E P W N F P F Y Q I A R V F I P N F I A G N

P M I L K D A S N C P A S A Q A F N D A V K E A G A P A G S

L T N L F L S Y D Q V N K A I A D K R V A G V C L T G S E R

G G A T V A K E A G A N L K K S T L E L G G N D A F I I L D

D A D W D L V E K V A P A A R L Y N A G Q V C T S S K R F I

V L E K D Y D R F L K M M K D A F S K V K M G D P L D P L T

T L A P L S S K K A K E K L Q Q Q V A T A V E N G A K V Y Y

G N K P V D M E G Q F F M P T I L T D I T P D N P I F D T E

M F G P V A S V Y K V S S E E E A I E L A N N S S Y G L G N

T I F S N D S E H A E R V A A K I E T G M S W I N A G W A S

L P E L P F G G V K N S G Y G R E L S S Y G I D E F T N K H

L I Y E A R Q

46.核苷酸序列，其由下述(40)所表示，并编码权利要求45所述的醛脱氢酶。

(40)ATGGCATATCAAAGTATCAATCCATTTACGAACCAAGTAGAAAAAACGTTTGAAAATACA

ACTGATGAAGAATTAGAACAAACATTAACTACGGCGCATCAATTATATTTAGATTGGCGG

AAGTATAATGACCTTGAAGAACGGAAACGGCAAATTTTAAAGTTAGGTCAAATATTACGT

GAACGGCGTGTTGAATATGCGACAGTTATGAGTAAGGAAATGGGAAAATTAATTAGCGAA

GCAGAAGGCGAGGTTGACCTTTGTGCTTCTTTCTGTGATTATTATGCAGCCCATGCAGAT

GAATTTCTGCAACCAAAAATTATTGCGACAACGAGTGGACGCGCCAAAGTTTTGAAGCAA

TCATTAGGAATTTTAGTTGCAGTTGAACCTTGGAATTTCCCATTCTATCAAATTGCCCGG

GTATTTATTCCCAACTTTATTGCAGGAAACCCCATGATCTTGAAGGATGCGTCGAATTGT

CCAGCATCCGCCCAAGCATTTAACGATGCCGTTAAGGAAGCTGGTGCGCCAGCCGGCAGT

TTAACTAATTTATTCCTTTCATATGACCAAGTAAATAAGGCAATTGCTGATAAGCGGGTA

GCCGGCGTTTGTCTTACTGGTTCTGAACGTGGTGGTGCAACCGTTGCTAAAGAGGCTGGT

GCTAATTTGAAGAAGAGCACTTTGGAACTTGGTGGTAATGATGCCTTTATTATCTTAGAC

GATGCAGATTGGGATCTTGTCGAAAAAGTTGCCCCGGCAGCCCGTCTGTATAATGCTGGA

CAAGTATGTACATCATCAAAACGTTTTATTGTCCTTGAAAAGGATTATGATCGTTTCTTA

AAGATGATGAAAGATGCGTTCTCGAAAGTTAAAATGGGTGATCCCCTTGATCCATTAACA

ACTCTGGCACCATTATCATCTAAGAAAGCAAAAGAAAAGCTCCAACAGCAAGTCGCAACA

GCAGTAGAAAATGGGGCCAAAGTTTACTATGGTAATAAGCCGGTTGACATGGAAGGTCAA

TTCTTTATGCCAACGATCTTAACTGATATCACTCCAGATAACCCAATATTTGATACGGAA

ATGTTTGGGCCAGTGGCTTCGGTTTATAAGGTTAGTTCCGAAGAGGAAGCAATCGAACTG

GCTAATAATTCAAGCTATGGGTTAGGAAACACTATCTTTAGCAATGATTCCGAACATGCG

GAACGAGTAGCAGCGAAGATCGAAACTGGAATGAGTTGGATTAATGCCGGCTGGGCTTCA

TTACCAGAATTACCATTTGGTGGTGTTAAGAATTCAGGTTACGGTCGTGAACTCAGCAGT

TACGGAATTGATGAATTTACTAACAAACATCTAATTTACGAAGCACGACAATAA

47.醛脱氢酶，其由下述(25)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示。

(25)M Q I N D I E S A V R K I L A E E L D N A S S S S A N V A A

T T D N G H R G I F T N V N D A I A A A K A A Q E I Y R D K

P I A V R Q Q V I D A I K E G F R P Y I E K M A K D I K E E

T G M G T V E A K I A K L N N A L Y N T P G P E I L E P V V

E N G D G G M V M Y E R L P Y G V I G A V G P S T N P S E T

V I A N A I M M L A G G N T L Y F G A H P G A K N V T R W T

I E K M N D F I A D A T G L H N L V V S I E T P T I E S V Q

Q M M K H P D I A M L A V T G G P A V V H Q A M T S G K K A

V G A G P G N P P A M V D A T A D I D L A A H N I I T S A S

F D N D I L C T A E K E V V A E S S I K D E L I R K M Q D E

G A F V V N R E Q A D K L A D M C I Q E N G A P D R K F V G

K D A T Y I L D Q A N I P Y T G H P V E I I C E L P K E H P

L V M T E M L M P I L P V V S C P T F D D V L K T A V E V E

K G N H H T A T I H S N N L K H I N N A A H R M Q C S I F V

V N G P S Y V G T G V A D N G A H S G A S A L T I A T P T G

E G T C T A R T F T R R V R L N S P Q G F S V R N W Y

48.核苷酸序列，其由下述(41)所表示，并编码权利要求47所述的醛脱氢酶。

(41)ATGCAGATTAATGATATTGAAAGTGCTGTACGCAAAATTCTTGCCGAAGAACTAGATAAT

GCCAGCTCTTCAAGTGCAAACGTTGCAGCTACTACTGATAATGGTCATCGCGGAATTTTC

ACTAATGTCAATGATGCAATTGCTGCTGCAAAAGCTGCTCAAGAAATATATCGGGATAAG

CCAATTGCTGTTCGCCAACAAGTGATTGATGCCATTAAGGAAGGATTCCGCCCATATATT

GAAAAAATGGCTAAAGATATCAAAGAAGAAACAGGAATGGGAACAGTAGAGGCCAAAATT

GCTAAGTTAAACAATGCCTTGTACAACACTCCTGGTCCCGAGATTCTTGAACCAGTTGTA

GAAAACGGTGACGGTGGGATGGTTATGTATGAACGGTTACCATATGGTGTTATTGGTGCG

GTTGGCCCAAGTACAAACCCTTCAGAAACTGTAATTGCTAATGCGATCATGATGCTTGCC

GGTGGTAATACTCTTTACTTTGGTGCTCACCCTGGCGCAAAGAATGTTACTCGCTGGACA

ATTGAAAAGATGAACGATTTTATTGCAGATGCAACAGGCCTTCATAATTTAGTTGTAAGT

ATTGAAACACCAACAATTGAATCAGTTCAACAAATGATGAAGCACCCCGACATTGCAATG

TTAGCAGTAACTGGTGGCCCAGCTGTTGTTCACCAAGCAATGACCAGTGGTAAGAAAGCG

GTTGGTGCTGGTCCTGGTAATCCTCCTGCAATGGTTGATGCTACTGCTGATATTGATTTA

GCTGCTCATAATATCATTACATCTGCTTCATTTGATAATGATATTTTATGTACTGCTGAA

AAGGAAGTAGTTGCAGAAAGTAGCATTAAAGATGAATTAATTCGTAAGATGCAAGATGAA

GGTGCCTTTGTAGTTAACCGTGAACAAGCCGATAAATTAGCTGATATGTGTATCCAAGAA

AATGGTGCTCCTGATCGTAAATTTGTTGGTAAGGATGCAACTTATATCTTAGACCAAGCT

AATATTCCTTACACAGGCCACCCAGTTGAAATTATTTGTGAACTTCCTAAGGAACATCCA

TTAGTAATGACTGAAATGTTAATGCCAATTTTACCAGTTGTTTCTTGTCCAACATTTGAT

GATGTTTTGAAGACTGCTGTTGAAGTTGAAAAAGGTAACCATCACACAGCTACTATTCAT

TCCAATAACCTTAAGCATATTAATAATGCTGCTCACCGGATGCAATGTTCAATCTTTGTT

GTTAATGGCCCATCCTATGTTGGTACAGGTGTTGCAGATAATGGAGCTCACTCAGGTGCT

TCAGCATTAACAATTGCTACGCCAACTGGTGAAGGAACATGTACTGCACGAACATTTACT

CGTCGGGTTCGTTTGAACTCACCACAAGGATTCTCAGTACGTAACTGGTATTAA

49.甘油酸激酶，其由下述(26)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示。

(26)M K F V I A P D S F K G G L T A K E A A N V M A E G I K R V

F P N A E Y A L V P M A D G G E G T V Q S L V D A T N G Q K

M I A K V H N P L N K L V N A E Y G I L G D G E T A V I E M

A A A S G L Q F V N K E T A N P L I T T T Y G T G E L I K D

A L D H N I K K I I I G I G G S A T V D G G A G M A Q A L G

A R L L D A D N H E I G L G G G E L A S L E Q V D F G G L D

P R L K N V D I Q I A S D V T N P L T G K N G A A P V F G P

Q K G A D E E M V N I L D K N L H H Y A R K I V A A G G P D

V E Q T A G A G A A G G L G A G L I A F T G A T M K R G V E

L V I E A T Q L Q K K A V G A D Y V F T G E G G I D F Q T K

F G K T P Y G V A K A T K E V A P T A P V I V L A G N I G K

G V N D L Y S S T A I D A I F A T P E G A K P L K T A L A D

A P I D I A Q T A E N V A R L I K V S H V S N

50.核苷酸序列，其由下述(42)所表示，并编码权利要求49所述的甘油酸激酶。

(42)ATGAAATTTGTAATTGCTCCAGATTCATTTAAAGGCGGATTAACAGCAAAAGAAGCAGCA

AATGTGATGGCAGAAGGAATCAAAAGAGTGTTTCCGAATGCCGAGTATGCTTTAGTTCCA

ATGGCTGATGGAGGAGAGGGGACTGTTCAATCCTTAGTTGATGCGACTAACGGTCAAAAA

ATGATTGCTAAAGTCCACAACCCATTAAATAAATTAGTTAATGCTGAGTACGGAATATTA

GGTGATGGGGAAACGGCAGTGATTGAGATGGCGGCGGCAAGTGGCCTTCAATTTGTTAAT

AAGGAGACTGCGAACCCGCTTATTACAACTACATATGGTACCGGCGAGTTAATTAAGGAT

GCTCTTGACCATAACATTAAAAAAATAATTATTGGAATTGGTGGAAGTGCAACCGTTGAT

GGCGGAGCGGGGATGGCCCAAGCACTTGGAGCACGTTTATTGGATGCTGATAATCATGAA

ATTGGTTTAGGCGGTGGTGAGTTAGCAAGTTTAGAGCAAGTAGATTTTGGAGGATTAGAT

CCTCGCTTAAAAAATGTAGATATTCAGATTGCATCAGACGTAACCAACCCATTAACAGGA

AAAAATGGGGCAGCCCCAGTATTTGGCCCGCAAAAAGGAGCTGATGAAGAAATGGTGAAC

ATCTTGGACAAAAATCTTCATCATTATGCCCGAAAAATAGTTGCAGCTGGTGGGCCAGAC

GTTGAACAAACGGCAGGTGCAGGGGCAGCCGGTGGTTTAGGAGCCGGGTTGATAGCATTT

ACCGGTGCGACAATGAAGCGAGGAGTAGAATTAGTGATTGAAGCAACTCAACTACAAAAA

AAGGCAGTTGGCGCTGATTATGTTTTTACTGGTGAAGGAGGAATTGATTTCCAGACTAAA

TTTGGTAAAACGCCATATGGAGTCGCTAAGGCAACTAAAGAGGTGGCTCCAACTGCTCCG

GTAATTGTGTTGGCTGGAAATATTGGTAAAGGCGTAAATGATCTATATTCATCCACGGCC

ATTGATGCAATTTTTGCAACTCCTGAAGGGGCTAAACCATTAAAAACAGCATTAGCAGAT

GCACCTATTGATATTGCTCAAACAGCGGAAAACGTTGCACGTTTAATTAAAGTGAGTCAT

GTTAGTAATTAA

51.甘油激酶，其由下述(27)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示。

(27)L S E Q Q Y I M A I D Q G T T S S R A I I F D H D G N K V A

I S Q Q E F P Q Y F P Q P G W V E H D P L E I W D S V Q S V

I S N V M I K S Q I K P Y K I A A I G I T N Q R E T T V I W

D R H T G K P I Y N A I V W Q S K Q T S D I A E Q L I K D G

Y K D M I H Q K T G L V I D S Y F A A T K I K W I L D H V P

G A R E K A A K G D L M F G T I D T W L L W N L S G R R V H

A T D V T N A S R T M L F N I H T L D W D Q D I L D L L D I

P Q S L L P V V K P S S A I Y G Y T G D Y H F Y G V Q I P I

A G I A G D Q Q A A L F G Q A A Y D K G S I K N T Y G T G A

F I V M N T G L K P T L S D N G L L T T I A Y G L D G Q T H

Y A L E G S I F V A G S A V Q W L R D G L K M F D K A S E S

E Q M A V D A K T T G G V Y V V P A F T G L G A P Y W D Q E

V R G A M F G L T R G T E R G H I I R A T L E A I A Y Q T K

D V V D T M V K D T Q L P L T A L T V N G G A S R N N F M M

Q F Q A D I L Q T P I K R A A M E E T T A L G A A F L A G L

A V D F W E D Q D E L R K L S R I G D Q F D P Q M D P Q K A

A D L Y R G W Q R A I A A A Q F Y G K D

52.核苷酸序列，其由下述(43)所表示，并编码权利要求51所述的甘油激酶。

(43)TTGAGTGAACAACAATATATCATGGCGATTGACCAGGGAACGACGAGCTCACGGGCGATT

ATCTTTGACCATGACGGAAATAAGGTTGCGATCAGTCAGCAGGAATTTCCCCAATACTTC

CCGCAGCCGGGGTGGGTTGAACATGATCCTCTAGAGATTTGGGATAGCGTTCAATCAGTG

ATTTCAAATGTAATGATTAAGTCCCAGATCAAGGCCTATAAGATTGCGGCAATTGGGATT

ACTAACCAACGGGAGACGACGGTTATTTGGGATCGCCATACCGGTAAGCCGATTTATAAC

GCAATTGTCTGGCAATCGAAGCAAACGAGCGACATCGCCGAACAATTGATTAAAGATGGT

TATAAGGATATGATCCACCAGAAGACTGGCTTGGTGATTGATTCGTATTTCGCGGCCACT

AAGATCAAGTGGATCCTTGACCATGTTCCTGGTGCCCGGGAAAAAGCAGCAAAGGGAGAC

TTGATGTTTGGGACTATCGATACTTGGTTACTATGGAATTTATCGGGACGGCGGGTCCAC

GCAACGGATGTGACCAATGCCAGCCGGACGATGCTTTTTAATATCCATACCCTCGACTGG

GATCAAGATATCCTTGACCTGCTTGATATTCCCCAGTCGCTTTTGCCAGTAGTAAAGCCA

AGTTCAGCCATTTACGGTTATACTGGCGACTACCACTTCTATGGGGTGCAGATTCCAATT

GCCGGGATTGCAGGTGACCAACAAGCAGCCCTCTTTGGTCAAGCAGCCTATGATAAAGGT

TCAATCAAGAACACCTATGGGACTGGAGCCTTCATCGTCATGAATACGGGACTAAAACCC

ACGCTTTCGGATAACGGCTTGTTGACGACGATTGCGTATGGCCTGGACGGGCAAACTCAT

TACGCGCTTGAAGGAAGTATCTTTGTGGCCGGTTCTGCCGTTCAATGGTTGCGGGATGGT

CTCAAGATGTTTGATAAGGCAAGCGAGTCCGAACAAATGGCTGTCGATGCCAAGACAACT

GGCGGCGTTTATGTCGTCCCCGCCTTTACAGGATTAGGCGCACCGTACTGGGATCAAGAA

GTGCGGGGCGCAATGTTTGGCCTTACCCGTGGAACTGAACGGGGACATATCATCCGTGCA

ACTTTGGAAGCCATTGCCTACCAGACCAAAGATGTTGTCGATACGATGGTCAAGGACACC

CAATTACCACTAACAGCACTAACGGTTAACGGGGGCGCTTCACGGAACAACTTCATGATG

CAGTTCCAGGCCGATATCTTACAAACGCCAATCAAGCGGGCAGCAATGGAAGAGACAACC

GCGCTGGGAGCAGCCTTTCTCGCTGGATTGGCCGTTGATTTCTGGGAAGACCAGGATGAG

TTACGGAAGCTATCACGGATTGGCGACCAGTTTGATCCACAAATGGATCCGCAAAAGGCA

GCTGACTTGTATCGGGGATGGCAACGGGCCATTGCAGCTGCGCAGTTTTATGGCAAAGAT

TAA

53.甘油-3-磷酸脱氢酶，其由下述(28)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示。

(28)M A E K I A V L G A G S W G S V L A N M L T E N G H D V T L

W S R N E E Q V K Q L N T E H T N P R Y M K D F V Y S T N L

T A T T D M K K A V K G A S V V L I V I P T K G L R E V A K

Q L N A I L T E L H Q K P L V I H A T K G L E Q N T Y K R P

S E M L S E D I S P E N R Q A I V V L S G P S H A E D V A I

K D M T A V T A A C E D L A S A K K A Q K L F S N S Y F R V

Y T N D D V I G A E F G A A L K N I I A I G A G A I Q G L G

Y H D N A R A A L I T R G L A E I R R L G V A F G A N P M T

F I G L S G V G D L V V T A T S K N S R N W R A G Y Q L G Q

G K K L Q D V I D N M G M V I E G V Y T T K A A Y E L S R K

R Q V Q M P I T E A L Y R V L Y E G E D I K T A I S Q L M D

R D L T S E N E

54.核苷酸序列，其由下述(44)所表示，并编码权利要求53所述的甘油-3-磷酸脱氢酶。

(44)ATGGCAGAAAAAATTGCTGTTTTAGGTGCTGGTTCGTGGGGCAGTGTTTTAGCAAACATG

CTTACAGAAAATGGCCACGATGTAACATTATGGTCTCGTAATGAGGAACAAGTTAAGCAA

TTAAATACTGAACATACAAATCCTCGCTATATGAAAGATTTTGTTTATTCTACTAACTTA

ACAGCAACAACGGACATGAAAAAAGCTGTTAAGGGTGCCAGTGTGGTCCTGATTGTAATT

CCAACAAAGGGTCTTCGTGAAGTTGCTAAGCAATTAAATGCAATTTTGACTGAATTACAT

CAAAAACCGCTAGTTATTCACGCAACGAAAGGCTTAGAACAAAATACTTATAAGCGGCCA

TCGGAAATGCTTAGCGAAGATATTTCTCCTGAAAACCGTCAGGCAATTGTTGTTTTATCA

GGTCCGAGTCATGCTGAAGATGTGGCGATTAAAGATATGACAGCTGTAACCGCAGCTTGT

GAGGACCTGGCCAGTGCTAAAAAGGCGCAGAAGTTATTTAGTAATTCTTATTTCCGTGTG

TACACTAATGACGATGTAATTGGTGCCGAATTTGGCGCAGCCTTAAAGAACATTATTGCA

ATTGGTGCTGGAGCTATTCAGGGACTTGGTTATCATGATAATGCTCGGGCAGCGTTAATT

ACTCGTGGACTTGCAGAAATTCGCCGATTGGGAGTTGCTTTTGGTGCCAACCCGATGACT

TTTATTGGTCTTTCTGGGGTTGGTGACCTTGTTGTTACTGCTACCAGTAAAAATTCTCGA

AATTGGCGTGCTGGCTATCAATTGGGGCAAGGAAAAAAGCTTCAAGATGTAATTGATAAT

ATGGGAATGGTTATCGAAGGTGTCTATACTACCAAAGCCGCTTATGAATTAAGTCGTAAA

CGACAAGTACAGATGCCAATTACCGAAGCTCTTTACCGTGTTTTGTATGAAGGCGAAGAT

ATTAAAACTGCAATTTCTCAATTAATGGACCGAGATCTTACTTCAGAAAACGAATAA

55.磷酸丙糖异构酶，其由下述(29)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示。

(29)M R K P F I A A N W K M H K N V Q E S V E F V D A I K G K L

P D P Q E V E V G I A A Q A F A L P S M V Q A A D D S G L K

I I A Q N A A A E Y S G A F T G E I S L R G L A D A G V S Y

V M L G H I E R R H L F H E D N E L V N R K V L A A L Q M G

V T P I I C T D E T M V Q K E V N G E I H Y V F Q Q L M S V

L R G V S L D Q I K N V V V S Y E P S W A V G Y G Q H A N P

V L A E E G C R Q I R R T I A D N Y T Y E I A D K I R I L Y

G G S V N P D N I G M I M N K P D V D G V L I G R A S L D V

D N F L R M V N Y L K N D Q E K

56.核苷酸序列，其由下述(45)所表示，并编码权利要求55所述的磷酸丙糖异构酶。

(45)ATGCGCAAACCCTTTATTGCTGCTAATTGGAAGATGCATAAGAATGTCCAAGAATCGGTT

GAATTTGTGGATGCAATTAAAGGAAAGCTACCAGATCCGCAAGAAGTTGAAGTCGGAATT

GCAGCCCAAGCTTTTGCATTACCCAGTATGGTTCAAGCCGCTGATGATTCAGGATTAAAG

ATAATCGCGCAAAACGCGGCGGCTGAATATTCGGGAGCTTTCACTGGTGAAATTAGCTTA

CGAGGTTTAGCTGACGCCGGTGTTTCATATGTAATGTTAGGACATATTGAACGGCGCCAT

TTATTCCACGAGGATAATGAGTTGGTTAATCGGAAAGTGTTGGCAGCCCTTCAAATGGGA

GTTACCCCGATAATTTGTACGGATGAAACGATGGTCCAGAAAGAAGTTAATGGTGAAATT

CACTACGTTTTCCAGCAATTGATGAGCGTATTGAGGGGCGTTTCTCTTGATCAAATTAAA

AATGTAGTTGTTTCCTATGAACCAAGTTGGGCAGTTGGATATGGTCAGCATGCTAATCCA

GTTCTTGCTGAAGAAGGATGCCGTCAAATTCGGCGAACGATTGCTGATAACTACACTTAT

GAGATTGCTGATAAGATCAGGATTCTTTATGGGGGCAGTGTCAATCCAGATAATATCGGA

ATGATTATGAACAAGCCAGATGTAGATGGGGTATTAATCGGTCGGGCAAGTTTAGATGTT

GATAATTTTTTGCGAATGGTCAATTATTTAAAAAATGATCAAGAAAAATAA

57.磷酸丙糖异构酶，其由下述(30)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示。

(30)M R K P F I I A N W K M N K N V H E S V A F V K A I K E K L

P A D K E I G I A A Q A V S L Y N M K K V A S S S N L Q I I

A Q N A S A E L E G P Y T G E I S M R S L A D A G V T Y V M

L G H L E R R R L F N E S N D S I N Q K V L A A L N A G I I

P I I C T D E E M V Q T E V N G Q I H Y V F R Q L K S V L K

G V P A N K L S Q I V I S Y E P S W A V G S T H Q A N P D I

A E E G C Q A I R Q S L V E M Y G N E I G E Q V R I L Y G G

S V N P E N I G Q I M S K P N V D G A L I G R A S L E I E S

F L Q M I N Y I E L A S K Q K L Q V I

58.核苷酸序列，其由下述(46)所表示，并编码权利要求57所述的磷酸丙糖异构酶。

(46)ATGCGCAAACCGTTTATTATTGCGAACTGGAAAATGAATAAAAACGTTCATGAATCTGTT

GCGTTTGTTAAAGCAATTAAAGAAAAGCTCCCGGCAGATAAAGAAATTGGGATCGCCGCG

CAAGCAGTTTCGCTATATAACATGAAAAAAGTGGCGAGCTCTTCCAACTTACAAATTATT

GCTCAAAATGCATCTGCTGAGTTAGAGGGACCATATACTGGAGAAATTAGCATGCGAAGT

TTAGCAGATGCGGGCGTGACATACGTGATGCTAGGCCATTTAGAGCGCCGACGCCTTTTT

AACGAGAGTAATGATTCAATTAATCAAAAAGTTTTAGCAGCCCTCAATGCTGGTATTATT

CCAATCATTTGTACGGATGAAGAGATGGTCCAAACAGAAGTTAACGGACAAATTCATTAT

GTATTTCGCCAACTAAAAAGCGTCCTTAAAGGGGTACCAGCTAATAAACTATCACAGATT

GTTATTTCGTATGAACCAAGTTGGGCCGTTGGGAGCACGCATCAAGCAAATCCAGACATT

GCGGAAGAGGGATGTCAGGCAATTCGTCAAAGCCTGGTTGAAATGTATGGTAATGAGATT

GGCGAGCAAGTCCGAATACTCTATGGTGGCAGCGTTAATCCCGAGAACATTGGTCAAATT

ATGAGTAAACCAAATGTTGATGGGGCGCTAATCGGTCGCGCAAGTCTCGAGATTGAAAGT

TTCTTACAAATGATTAATTATATCGAATTAGCGAGCAAGCAGAAGTTACAGGTAATTTAG

59.磷酸丙糖异构酶，其由下述(31)所示的氨基酸序列或该氨基酸序列中1个或多个氨基酸缺失、置换或增加的氨基酸序列所表示。

(31)M R V P I I A G N W K M H K D V Q E A V S F I E K V K N Q L

P P A D Q L E T A I A A P T L C L V P M V K A A E E S P L K

I M A E N C Y Y K N E G A Y T G E T S P Y A L Y Q A G I H H

V I L G H S E R R T Y F N E T D E L I N K K V K A A L V N G

L C P I V C C D D T M R R R V A G K K V H W V V S R I L A D

L H G L T N D E I C H V T V A Y E P S W A I G T G E S A D P

E Q A A E G C Y L I R Q T I S D M Y G D E V A N N V R I L Y

G G S V T T S N I N A L M A K N D I D G V L V G A A S L N P

E T F L Q L V H H

60.核苷酸序列，其由下述(47)所表示，并编码权利要求59所述的磷酸丙糖异构酶。

(47)ATGAGAGTACCGATTATTGCTGGTAATTGGAAAATGCATAAGGATGTACAAGAAGCTGTC

TCTTTTATCGAAAAAGTAAAAAATCAGCTTCCGCCTGCCGACCAACTTGAAACAGCAATT

GCTGCTCCTACTCTTTGTTTAGTACCAATGGTTAAAGCAGCTGAAGAATCCCCGTTAAAA

ATAATGGCAGAAAACTGCTACTATAAGAATGAGGGAGCTTATACTGGTGAAACAAGTCCA

TATGCTTTATACCAAGCAGGAATCCATCATGTGATTTTAGGCCATTCTGAACGCCGAACT

TACTTTAATGAAACTGATGAATTAATTAATAAAAAAGTGAAGGCAGCATTAGTAAATGGG

TTATGTCCGATTGTTTGTTGTGATGATACTATGCGTCGACGAGTTGCTGGAAAGAAAGTT

CATTGGGTGGTGAGCCGAATTCTCGCTGACCTTCATGGATTGACCAATGACGAAATTTGT

CATGTTACGGTTGCTTATGAACCAAGTTGGGCGATTGGAACAGGCGAGAGTGCTGATCCA

GAACAAGCGGCGGAAGGTTGTTACCTTATTCGGCAAACGATTAGTGATATGTATGGCGAT

GAAGTTGCAAATAACGTTCGAATTCTCTATGGCGGAAGTGTGACAACTTCTAATATCAAT

GCACTAATGGCAAAAAATGATATTGATGGTGTTTTAGTCGGAGCGGCGAGCTTAAATCCA

GAAACATTTTTACAATTAGTTCACCATTAG