CN101218247A - 利用hcic和离子交换层析的蛋白质纯化 - Google Patents

利用hcic和离子交换层析的蛋白质纯化 Download PDF

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Publication number
CN101218247A
CN101218247A CNA2006800166104A CN200680016610A CN101218247A CN 101218247 A CN101218247 A CN 101218247A CN A2006800166104 A CNA2006800166104 A CN A2006800166104A CN 200680016610 A CN200680016610 A CN 200680016610A CN 101218247 A CN101218247 A CN 101218247A
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antibody
target protein
chromatography
electric charge
mixture
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CNA2006800166104A
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English (en)
Chinese (zh)
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A·阿鲁纳库马里
G·M·M·费赖拉
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ER Squibb and Sons LLC
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Medarex LLC
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39516—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum from serum, plasma
    • A61K39/39525—Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2878—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CNA2006800166104A 2005-04-11 2006-03-21 利用hcic和离子交换层析的蛋白质纯化 Pending CN101218247A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US67045705P 2005-04-11 2005-04-11
US60/670,457 2005-04-11

Publications (1)

Publication Number Publication Date
CN101218247A true CN101218247A (zh) 2008-07-09

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CNA2006800166104A Pending CN101218247A (zh) 2005-04-11 2006-03-21 利用hcic和离子交换层析的蛋白质纯化

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Country Link
US (3) US8129508B2 (https=)
EP (1) EP1869067A1 (https=)
JP (1) JP2008535913A (https=)
KR (1) KR20080006601A (https=)
CN (1) CN101218247A (https=)
AU (1) AU2006234909A1 (https=)
CA (1) CA2604877A1 (https=)
EA (1) EA013504B1 (https=)
MX (1) MX2007012499A (https=)
NZ (1) NZ562949A (https=)
WO (1) WO2006110277A1 (https=)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103028376A (zh) * 2011-09-29 2013-04-10 佛山市博新生物科技有限公司 一种用于清除血液毒素的血液净化吸附剂及其制备方法
CN105777896A (zh) * 2015-03-19 2016-07-20 广东东阳光药业有限公司 一种抗体酸性峰的纯化方法
CN107413312A (zh) * 2017-07-18 2017-12-01 天津工业大学 一种用于抗体纯化的混合模式蛋白吸附膜及制备方法
CN107922476A (zh) * 2015-03-13 2018-04-17 诺夫免疫股份有限公司 纯化双特异性抗体的方法
CN108239146A (zh) * 2018-03-26 2018-07-03 江苏中新医药有限公司 一种高纯度rhNGF的制备方法
CN110348090A (zh) * 2019-06-28 2019-10-18 浙江大学 基于人工神经网络实现多柱连续流层析设计及分析的方法
CN111712510A (zh) * 2017-12-21 2020-09-25 建新公司 用于在蛋白a色谱法期间增强杂质去除的方法
CN111991571A (zh) * 2020-08-12 2020-11-27 湖州师范学院 一种柱上低pH病毒灭活的方法
CN119113574A (zh) * 2024-09-10 2024-12-13 宝利化（南京）制药有限公司 一种熊去氧胆酸制备纯化装置、方法及其应用

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CN102887955A (zh) 2006-04-05 2013-01-23 艾博特生物技术有限公司 抗体纯化
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FR2910786B1 (fr) * 2006-12-29 2017-08-11 Laboratoire Francais Du Fractionnement Et Des Biotechnologies (Lfb) "procede d'extraction d'une proteine presente dans du lait"
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JP6010030B2 (ja) * 2010-09-20 2016-10-19 アッヴィ・インコーポレイテッド 疑似移動床クロマトグラフィーを使用する抗体の精製
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CN102127164B (zh) 2010-12-20 2013-01-30 武汉禾元生物科技有限公司 一种从水稻种子中提取重组人血清白蛋白的方法
KR101574864B1 (ko) * 2010-12-21 2015-12-11 에프. 호프만-라 로슈 아게 이소폼이 농축된 항체 제제 및 그의 제조 방법
CN102532254B (zh) * 2010-12-24 2015-06-24 武汉禾元生物科技股份有限公司 一种从水稻种子中分离纯化重组人血清白蛋白的方法
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KR101569783B1 (ko) 2013-06-05 2015-11-19 한화케미칼 주식회사 항체의 정제 방법
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CN120904322A (zh) 2013-09-13 2025-11-07 豪夫迈·罗氏有限公司 包含纯化的重组多肽的方法和组合物
RU2016107435A (ru) * 2013-09-13 2017-10-18 Дженентек, Инк. Композиции и способы обнаружения и количественного определения белка клеток-хозяев в клеточных линиях и рекомбинантные полипептидные продукты
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Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103028376A (zh) * 2011-09-29 2013-04-10 佛山市博新生物科技有限公司 一种用于清除血液毒素的血液净化吸附剂及其制备方法
CN114249831A (zh) * 2015-03-13 2022-03-29 诺夫免疫股份有限公司 纯化双特异性抗体的方法
CN107922476A (zh) * 2015-03-13 2018-04-17 诺夫免疫股份有限公司 纯化双特异性抗体的方法
CN105777896B (zh) * 2015-03-19 2019-08-16 广东东阳光药业有限公司 一种抗体酸性峰的纯化方法
CN105777896A (zh) * 2015-03-19 2016-07-20 广东东阳光药业有限公司 一种抗体酸性峰的纯化方法
CN107413312A (zh) * 2017-07-18 2017-12-01 天津工业大学 一种用于抗体纯化的混合模式蛋白吸附膜及制备方法
CN111712510A (zh) * 2017-12-21 2020-09-25 建新公司 用于在蛋白a色谱法期间增强杂质去除的方法
CN111712510B (zh) * 2017-12-21 2024-06-14 建新公司 用于在蛋白a色谱法期间增强杂质去除的方法
US12528835B2 (en) 2017-12-21 2026-01-20 Genzyme Corporation Methods for enhanced removal of impurities during protein a chromatography
CN108239146A (zh) * 2018-03-26 2018-07-03 江苏中新医药有限公司 一种高纯度rhNGF的制备方法
CN110348090A (zh) * 2019-06-28 2019-10-18 浙江大学 基于人工神经网络实现多柱连续流层析设计及分析的方法
CN111991571A (zh) * 2020-08-12 2020-11-27 湖州师范学院 一种柱上低pH病毒灭活的方法
CN119113574A (zh) * 2024-09-10 2024-12-13 宝利化（南京）制药有限公司 一种熊去氧胆酸制备纯化装置、方法及其应用
CN119113574B (zh) * 2024-09-10 2025-04-18 宝利化（南京）制药有限公司 一种熊去氧胆酸制备纯化装置、方法及其应用

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KR20080006601A (ko) 2008-01-16
CA2604877A1 (en) 2006-10-19
US20090105465A1 (en) 2009-04-23
EP1869067A1 (en) 2007-12-26
US8697847B2 (en) 2014-04-15
WO2006110277A1 (en) 2006-10-19
US20120165511A1 (en) 2012-06-28
NZ562949A (en) 2009-05-31
EA200702210A1 (ru) 2008-04-28
US8129508B2 (en) 2012-03-06
AU2006234909A1 (en) 2006-10-19
MX2007012499A (es) 2007-12-06
JP2008535913A (ja) 2008-09-04
EA013504B1 (ru) 2010-06-30
US20140179904A1 (en) 2014-06-26

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