NZ562949A - Protein purification using HCIC and ion exchange chromatography - Google Patents

Protein purification using HCIC and ion exchange chromatography

Info

Publication number
NZ562949A
NZ562949A NZ562949A NZ56294906A NZ562949A NZ 562949 A NZ562949 A NZ 562949A NZ 562949 A NZ562949 A NZ 562949A NZ 56294906 A NZ56294906 A NZ 56294906A NZ 562949 A NZ562949 A NZ 562949A
Authority
NZ
New Zealand
Prior art keywords
protein
chromatography
antibody
target protein
range
Prior art date
Application number
NZ562949A
Other languages
English (en)
Inventor
Alahari Arunakumari
Gisela Maria Marques Ferreira
Original Assignee
Medarex Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medarex Inc filed Critical Medarex Inc
Publication of NZ562949A publication Critical patent/NZ562949A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39516—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum from serum, plasma
    • A61K39/39525—Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2878—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
NZ562949A 2005-04-11 2006-03-21 Protein purification using HCIC and ion exchange chromatography NZ562949A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US67045705P 2005-04-11 2005-04-11
PCT/US2006/010412 WO2006110277A1 (en) 2005-04-11 2006-03-21 Protein purification using hcic amd ion exchange chromatography

Publications (1)

Publication Number Publication Date
NZ562949A true NZ562949A (en) 2009-05-31

Family

ID=36764783

Family Applications (1)

Application Number Title Priority Date Filing Date
NZ562949A NZ562949A (en) 2005-04-11 2006-03-21 Protein purification using HCIC and ion exchange chromatography

Country Status (11)

Country Link
US (3) US8129508B2 (https=)
EP (1) EP1869067A1 (https=)
JP (1) JP2008535913A (https=)
KR (1) KR20080006601A (https=)
CN (1) CN101218247A (https=)
AU (1) AU2006234909A1 (https=)
CA (1) CA2604877A1 (https=)
EA (1) EA013504B1 (https=)
MX (1) MX2007012499A (https=)
NZ (1) NZ562949A (https=)
WO (1) WO2006110277A1 (https=)

Families Citing this family (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1869067A1 (en) 2005-04-11 2007-12-26 Medarex, Inc. Protein purification using hcic amd ion exchange chromatography
CN102887955A (zh) 2006-04-05 2013-01-23 艾博特生物技术有限公司 抗体纯化
SI2061803T2 (sl) * 2006-08-28 2023-01-31 Ares Trading S.A. Postopek za čiščenje proteinov, ki vsebujejo FC
FR2910786B1 (fr) * 2006-12-29 2017-08-11 Laboratoire Francais Du Fractionnement Et Des Biotechnologies (Lfb) "procede d'extraction d'une proteine presente dans du lait"
EP2102335B1 (en) * 2007-01-04 2012-02-22 Crucell Holland B.V. Purification of factor xi
KR100955470B1 (ko) * 2008-01-09 2010-04-30 대한민국(관리부서 질병관리본부장) 탄저 방어 항원의 제조 방법
WO2010031007A2 (en) 2008-09-12 2010-03-18 Genvault Corporation Matrices and media for storage and stabilization of biomolecules
BRPI0920572A8 (pt) 2008-10-20 2015-10-27 Abbott Lab Inativação viral durante a purificação dos anticorpos
MX2011004199A (es) * 2008-10-20 2011-05-24 Abbott Lab Anticuerpos que unen a il-18 y metodos para purificar los mismos.
NZ592095A (en) 2008-10-20 2013-01-25 Abbott Lab Isolation and purification of il-12 and tnf-alpha antibodies using protein a affinity chromatography
BRPI0919879A2 (pt) * 2008-10-29 2016-02-16 Wyeth Llc métodos para purificação de moléculas de ligação a antígeno de domínio único
WO2010151632A1 (en) 2009-06-25 2010-12-29 Bristol-Myers Squibb Company Protein purifacation by caprylic acid (octanoic acid ) precipitation
WO2011073235A1 (en) 2009-12-18 2011-06-23 Csl Ltd. Method of purifying polypeptides
KR101976853B1 (ko) 2010-05-25 2019-05-09 제넨테크, 인크. 폴리펩티드의 정제 방법
JP6010030B2 (ja) * 2010-09-20 2016-10-19 アッヴィ・インコーポレイテッド 疑似移動床クロマトグラフィーを使用する抗体の精製
CA2816190C (en) * 2010-10-27 2020-03-24 Philip Morris Products S.A. Methods for capturing virus like particles from plants using expanded bed chromatography
CN102127164B (zh) 2010-12-20 2013-01-30 武汉禾元生物科技有限公司 一种从水稻种子中提取重组人血清白蛋白的方法
KR101574864B1 (ko) * 2010-12-21 2015-12-11 에프. 호프만-라 로슈 아게 이소폼이 농축된 항체 제제 및 그의 제조 방법
CN102532254B (zh) * 2010-12-24 2015-06-24 武汉禾元生物科技股份有限公司 一种从水稻种子中分离纯化重组人血清白蛋白的方法
US9062106B2 (en) 2011-04-27 2015-06-23 Abbvie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
WO2013015738A1 (en) * 2011-07-22 2013-01-31 HJERTÉN, Maria Capture of pathogenic and non-pathogenic biopolymers and bioparticles
CN103028376B (zh) * 2011-09-29 2015-05-13 佛山市博新生物科技有限公司 一种用于清除血液毒素的血液净化吸附剂及其制备方法
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
WO2013176754A1 (en) 2012-05-24 2013-11-28 Abbvie Inc. Novel purification of antibodies using hydrophobic interaction chromatography
WO2014004103A1 (en) 2012-06-29 2014-01-03 Emd Millipore Corporation Methods for inactivating viruses during a protein purification process
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
HK1211981A1 (en) 2012-09-02 2016-06-03 Abbvie Inc. Methods to control protein heterogeneity
CN103880947B (zh) 2012-12-21 2016-01-13 武汉禾元生物科技股份有限公司 一种分离纯化高纯度重组人血清白蛋白的层析方法
EP2958995A4 (en) * 2013-02-22 2016-11-23 Agency Science Tech & Res CHROMATOGRAPHIC CLEANING OF VIRUS PREPARATIONS WITH NEGATIVELY LOADED PARTICLES
HK1207960A1 (en) 2013-03-12 2016-02-19 Abbvie Inc. Human antibodies that bind human tnf-alpha and methods of preparing the same
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
WO2014159579A1 (en) 2013-03-14 2014-10-02 Abbvie Inc. MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE
US9499614B2 (en) 2013-03-14 2016-11-22 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
KR101569783B1 (ko) 2013-06-05 2015-11-19 한화케미칼 주식회사 항체의 정제 방법
US10076749B2 (en) 2013-08-23 2018-09-18 Boehringer Ingelheim Rcv Gmbh & Co Kg Microparticles for cell disruption and/or biomolecule recovery
US20150093800A1 (en) * 2013-09-05 2015-04-02 Genentech, Inc. Method for chromatography reuse
CN120904322A (zh) 2013-09-13 2025-11-07 豪夫迈·罗氏有限公司 包含纯化的重组多肽的方法和组合物
RU2016107435A (ru) * 2013-09-13 2017-10-18 Дженентек, Инк. Композиции и способы обнаружения и количественного определения белка клеток-хозяев в клеточных линиях и рекомбинантные полипептидные продукты
JPWO2015041218A1 (ja) 2013-09-17 2017-03-02 株式会社カネカ 新規抗体精製方法及びそれから得られる抗体（ＮｏｖｅｌＡｎｔｉｂｏｄｙＰｕｒｉｆｉｃａｔｉｏｎＭｅｔｈｏｄａｎｄＡｎｔｉｂｏｄｙｏｂｔａｉｎｅｄｔｈｅｒｅｆｒｏｍ）、並びに陽イオン交換基を用いた新規抗体精製法及びそれから得られる抗体（ＮｏｖｅｌＡｎｔｉｂｏｄｙＰｕｒｉｆｉｃａｔｉｏｎｍｅｔｈｏｄｕｓｉｎｇＣａｔｉｏｎＥｘｃｈａｎｇｅｒａｎｄＡｎｔｉｂｏｄｙｏｂｔａｉｎｅｄｔｈｅｒｅｆｒｏｍ）
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
AU2015249902B2 (en) * 2014-04-23 2019-01-31 Alexion Pharmaceuticals, Inc. Egg white processing
CN105296433B (zh) 2014-08-01 2018-02-09 中山康方生物医药有限公司 一种ctla4抗体、其药物组合物及其用途
US10738078B2 (en) 2014-11-03 2020-08-11 Bristol-Myers Squibb Company Use of caprylic acid precipitation for protein purification
ES3006358T3 (en) * 2015-03-13 2025-03-18 Novimmune Sa Methods of purifying bispecific antibodies
CN105777896B (zh) * 2015-03-19 2019-08-16 广东东阳光药业有限公司 一种抗体酸性峰的纯化方法
WO2018035710A1 (en) 2016-08-23 2018-03-01 Akeso Biopharma, Inc. Anti-ctla4 antibodies
CN107413312A (zh) * 2017-07-18 2017-12-01 天津工业大学 一种用于抗体纯化的混合模式蛋白吸附膜及制备方法
MA49962A (fr) 2017-08-22 2020-07-01 Biogen Ma Inc Procédés de purification d'anticorps présentant des agrégats réduits à poids moléculaire élevé
KR102753090B1 (ko) * 2017-12-21 2025-01-14 젠자임 코포레이션 단백질 a 크로마토그래피 동안 불순물의 향상된 제거를 위한 방법
CN108239146A (zh) * 2018-03-26 2018-07-03 江苏中新医药有限公司 一种高纯度rhNGF的制备方法
CN109021066A (zh) * 2018-08-23 2018-12-18 上海青平药业有限公司 一种发酵液提取谷胱甘肽的方法
WO2020067027A1 (ja) * 2018-09-28 2020-04-02 一般財団法人阪大微生物病研究会 Ｖｌｐ発現ｃｈｏ細胞株の構築
CN110348090B (zh) * 2019-06-28 2021-05-04 浙江大学 基于人工神经网络实现多柱连续流层析设计及分析的方法
KR102170372B1 (ko) 2019-08-13 2020-10-27 주식회사 세이포드 외이도 내 인체 조직에 음향 전달을 위한 사운드 앵커 및 이를 구비한 반이식형 보청기
WO2022018619A1 (en) * 2020-07-20 2022-01-27 Purolite (China) Co., Ltd. Methods for chromatographic protein extraction and purification
CN111991571B (zh) * 2020-08-12 2022-04-05 湖州师范学院 一种柱上低pH病毒灭活的方法
CN112094816B (zh) * 2020-09-23 2021-06-04 绿城农科检测技术有限公司 分泌2-巯基苯并咪唑单克隆抗体的杂交瘤细胞株、单克隆抗体及应用
CA3247416A1 (en) * 2022-04-08 2023-10-12 Csl Behring Ag Methods of sanitizing and/or regenerating a chromatography medium
CN119113574B (zh) * 2024-09-10 2025-04-18 宝利化（南京）制药有限公司 一种熊去氧胆酸制备纯化装置、方法及其应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7138262B1 (en) * 2000-08-18 2006-11-21 Shire Human Genetic Therapies, Inc. High mannose proteins and methods of making high mannose proteins
US20040018194A1 (en) * 2000-11-28 2004-01-29 Francisco Joseph A. Recombinant anti-CD30 antibodies and uses thereof
EP2295081B1 (en) * 2001-06-26 2018-10-31 Amgen Inc. Antibodies to OPGL
WO2003102132A2 (en) * 2002-04-26 2003-12-11 Genetech, Inc. Non-affinity purification of proteins
HUE033623T2 (en) * 2002-09-11 2017-12-28 Genentech Inc protein purification
DK1689777T3 (da) 2003-11-05 2007-06-11 Ares Trading Sa Fremgangsmåde til oprensning af il18-bindende protein
EP1869067A1 (en) 2005-04-11 2007-12-26 Medarex, Inc. Protein purification using hcic amd ion exchange chromatography
CA2645739A1 (en) * 2006-03-20 2007-09-27 Medarex, Inc. Protein purification

Also Published As

Publication number Publication date
KR20080006601A (ko) 2008-01-16
CA2604877A1 (en) 2006-10-19
US20090105465A1 (en) 2009-04-23
EP1869067A1 (en) 2007-12-26
CN101218247A (zh) 2008-07-09
US8697847B2 (en) 2014-04-15
WO2006110277A1 (en) 2006-10-19
US20120165511A1 (en) 2012-06-28
EA200702210A1 (ru) 2008-04-28
US8129508B2 (en) 2012-03-06
AU2006234909A1 (en) 2006-10-19
MX2007012499A (es) 2007-12-06
JP2008535913A (ja) 2008-09-04
EA013504B1 (ru) 2010-06-30
US20140179904A1 (en) 2014-06-26

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