CN101048512A - 抗淀粉样β肽抗体的生产 - Google Patents
抗淀粉样β肽抗体的生产 Download PDFInfo
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- CN101048512A CN101048512A CNA2005800368972A CN200580036897A CN101048512A CN 101048512 A CN101048512 A CN 101048512A CN A2005800368972 A CNA2005800368972 A CN A2005800368972A CN 200580036897 A CN200580036897 A CN 200580036897A CN 101048512 A CN101048512 A CN 101048512A
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- glutamine
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Abstract
Description
培养基1 | 培养基2 | 培养基3 | ||||
氨基酸 | mg/L | mM | mg/L | mM | mg/L | mM |
丙氨酸 | 96.03 | 1.08 | 17.80 | 0.20 | 24.87 | 0.28 |
精氨酸 | 1186.99 | 6.82 | 347.97 | 2.00 | 423.43 | 2.43 |
天冬酰胺一水合物 | 713.59 | 4.76 | 75.00 | 0.50 | 173.90 | 1.16 |
天冬氨酸 | 318.53 | 2.39 | 26.20 | 0.20 | 52.72 | 0.40 |
半胱氨酸盐酸盐一水合物 | 70.01 | 0.40 | 70.19 | 0.40 | 70.01 | 0.40 |
胱氨酸二盐酸盐 | 297.09 | 0.95 | 62.25 | 0.20 | 62.09 | 0.20 |
谷氨酸 | 158.59 | 1.08 | 29.40 | 0.20 | 41.08 | 0.28 |
谷氨酰胺 | 1892.40 | 12.96 | 1163.95 | 7.97 | 1162.40 | 7.96 |
甘氨酸 | 95.88 | 1.28 | 30.00 | 0.40 | 35.92 | 0.48 |
组氨酸盐酸盐一水合物 | 369.10 | 1.76 | 46.00 | 0.22 | 75.27 | 0.36 |
异亮氨酸 | 623.63 | 4.76 | 104.99 | 0.80 | 151.90 | 1.16 |
亮氨酸 | 852.31 | 6.51 | 104.99 | 0.80 | 172.69 | 1.32 |
盐酸赖氨酸 | 945.96 | 5.20 | 145.99 | 0.80 | 218.38 | 1.20 |
甲硫氨酸 | 291.82 | 1.96 | 29.80 | 0.20 | 53.55 | 0.36 |
苯丙氨酸 | 428.62 | 2.60 | 65.99 | 0.40 | 98.81 | 0.60 |
脯氨酸 | 372.25 | 3.24 | 68.99 | 0.60 | 96.40 | 0.84 |
丝氨酸 | 904.71 | 8.62 | 126.00 | 1.20 | 273.07 | 2.60 |
苏氨酸 | 513.39 | 4.31 | 94.99 | 0.80 | 132.81 | 1.12 |
色氨酸 | 159.32 | 0.78 | 16.00 | 0.08 | 28.99 | 0.14 |
二水合二钠酪氨酸 | 560.81 | 2.15 | 103.79 | 0.40 | 145.10 | 0.56 |
缬氨酸 | 505.36 | 4.32 | 93.99 | 0.80 | 131.17 | 1.12 |
维生素 | mg/L | μM | mg/L | μM | mg/L | μM |
生物素 | 2.00 | 8.21 | 0.20 | 0.82 | 0.36 | 1.49 |
泛酸钙 | 22.02 | 46.27 | 2.24 | 4.71 | 4.03 | 8.47 |
氯化胆碱 | 87.67 | 630.74 | 8.98 | 64.60 | 16.11 | 115.92 |
叶酸 | 25.95 | 58.84 | 2.65 | 6.01 | 4.76 | 10.80 |
肌醇 | 123.39 | 685.47 | 12.60 | 69.99 | 22.64 | 125.79 |
烟酰胺 | 19.60 | 160.70 | 2.02 | 16.56 | 3.61 | 29.62 |
盐酸吡哆醛 | 1.99 | 9.83 | 2.00 | 9.85 | 1.99 | 9.83 |
盐酸吡哆醇 | 18.06 | 87.67 | 0.03 | 0.15 | 1.67 | 8.10 |
核黄素 | 2.20 | 5.85 | 0.22 | 0.59 | 0.40 | 1.06 |
盐酸硫胺素(维生素B1) | 21.51 | 63.84 | 2.17 | 6.44 | 3.92 | 11.64 |
维生素B12 | 6.93 | 5.12 | 0.78 | 0.58 | 1.34 | 0.99 |
无机盐 | mg/L | mM | mg/L | mM | mg/L | mM |
CaCl2 | 115.78 | 1.04 | 116.09 | 1.05 | 115.78 | 1.04 |
KCl | 310.94 | 4.17 | 311.77 | 4.18 | 310.94 | 4.17 |
Na2HPO4 | 70.81 | 0.50 | 70.99 | 0.50 | 70.81 | 0.50 |
NaCl | 1104.96 | 18.92 | 5539.00 | 94.85 | 3704.96 | 63.44 |
NaH2PO4·H2O | 636.33 | 4.61 | 62.49 | 0.45 | 114.33 | 0.83 |
MgSO4 | 48.70 | 0.41 | 48.83 | 0.41 | 48.70 | 0.41 |
MgSO4·7H2O | 0.03 | 95.00 | 8.60 | 95.00 | ||
MgCl2 | 28.53 | 0.30 | 28.61 | 0.30 | 28.53 | 0.30 |
NaHCO3 | 2000.00 | 23.81 | 2440.00 | 29.04 | 2440.00 | 29.04 |
微量元素 | μg/L | nM | μg/L | nM | μg/L | nM |
亚硒酸钠 | 28.00 | 161.94 | 5.00 | 28.92 | 7.00 | 40.49 |
Fe(NO3)3·9H2O | 49.86 | 123.42 | 50.00 | 123.75 | 49.86 | 123.42 |
CuSO4 | 2.69 | 16.80 | 0.80 | 5.00 | 0.97 | 6.06 |
CuSO4·5H2O | 11.24 | 45.00 | 7.49 | 30.00 | ||
FeSO4·7H2O | 2503.85 | 9006.64 | 839.96 | 3021.45 | 1542.85 | 5549.81 |
ZnSO4·7H2O | 2734.77 | 9528.82 | 429.96 | 1498.12 | 1383.80 | 4821.59 |
MnSO4·H2O | 0.26 | 1.51 | 0.17 | 1.01 | ||
Na2SiO3·9H2O | 210.00 | 739.27 | 140.00 | 492.84 | ||
(NH4)6Mo7O24·4H2O | 1.86 | 1.50 | 1.24 | 1.00 | ||
NH4VO3 | 0.98 | 8.33 | 0.65 | 5.56 | ||
NiSO4·6H2O | 0.20 | 0.74 | 0.13 | 0.49 | ||
SnCl2·2H2O | 0.18 | 0.80 | 0.12 | 0.53 | ||
其它成分 | mg/L | μM | mg/L | μM | mg/L | μM |
氢化可的松 | 0.23 | 0.64 | 0.04 | 0.10 | 0.09 | 0.24 |
二盐酸腐胺 | 6.48 | 40.22 | 1.08 | 6.70 | 2.48 | 15.39 |
亚油酸 | 0.22 | 0.80 | 0.04 | 0.14 | 0.06 | 0.20 |
硫辛酸 | 0.56 | 2.73 | 0.10 | 0.49 | 0.14 | 0.69 |
D-葡萄糖(右旋糖) | 16039.43 | 89107.92 | 6150.72 | 34170.64 | 11042.24 | 61345.76 |
PVA | 2560.00 | 2400.00 | 2520.00 | |||
胰岛素 | 54.00 | 10.00 | 14.00 | |||
丙酮酸钠 | 54.85 | 498.63 | 55.00 | 499.95 | 54.85 | 498.63 |
培养基4A-1 | 培养基4B | 微量元素B | 微量元素D | |||||
氨基酸 | mg/L | mM | mg/L | mM | mg/L | mM | mg/L | mM |
丙氨酸 | 17.80 | 0.20 | ||||||
精氨酸 | 191.00 | 1.10 | ||||||
天冬酰胺一水合物 | 135.00 | 0.90 | ||||||
天冬氨酸 | 66.50 | 0.50 | ||||||
谷氨酸 | 29.40 | 0.20 | ||||||
甘氨酸 | 15.00 | 0.20 | ||||||
组氨酸盐酸盐一水合物 | 73.50 | 0.35 | ||||||
异亮氨酸 | 118.00 | 0.90 | ||||||
亮氨酸 | 170.00 | 1.30 | ||||||
盐酸赖氨酸 | 182.00 | 1.00 | ||||||
甲硫氨酸 | 59.60 | 0.40 | ||||||
苯丙氨酸 | 82.50 | 0.50 | ||||||
脯氨酸 | 69.00 | 0.60 | ||||||
丝氨酸 | 158.00 | 1.50 | ||||||
苏氨酸 | 95.20 | 0.80 | ||||||
色氨酸 | 32.60 | 0.16 | ||||||
二水合酪氨酸二钠 | 104.00 | 0.40 |
缬氨酸 | 93.60 | 0.80 | ||||||
维生素 | mg/L | μM | mg/L | μM | mg/L | mM | mg/L | mM |
生物素 | 0.41 | 1.68 | ||||||
泛酸钙 | 4.50 | 9.45 | ||||||
氯化胆碱 | 17.90 | 128.78 | ||||||
叶酸 | 5.30 | 12.02 | ||||||
肌醇 | 25.20 | 140.00 | ||||||
烟酰胺 | 4.00 | 32.79 | ||||||
盐酸吡哆醇 | 4.10 | 19.90 | ||||||
核黄素 | 0.45 | 1.20 | ||||||
盐酸硫胺素 | 4.40 | 13.06 | ||||||
维生素B12 | 1.40 | 1.03 | ||||||
微量元素 | μg/L | nM | mg/L | μM | μg/L | nM | μg/L | nM |
(NH4)6Mo7O24·4H2O | 1.24 | 1.00 | ||||||
CuSO4 | 0.43 | 2.69 | ||||||
CuSO4·5H2O | 7.49 | 30.00 | ||||||
FeSO4·7H2O | 834 | 3000 | ||||||
MnSO4·H2O | 0.17 | 1.01 | ||||||
Na2SiO3·9H2O | 140.00 | 492.84 | ||||||
NH4VO3 | 0.65 | 5.56 | ||||||
NiSO4·6H2O | 0.13 | 0.49 | ||||||
SnCl2·2H2O | 0.12 | 0.53 | ||||||
ZnSO4·7H2O | 230.00 | 801.39 | 863 | 3007 | ||||
其它成分 | μg/L | nM | μg/L | nM | μg/L | nM | μg/L | nM |
亚油酸 | 42.00 | 0.15 | ||||||
硫辛酸 | 105.00 | 0.51 | ||||||
D-葡萄糖(右旋糖) | 1000000 | 5555.56 |
部分 | 成分 | 含量 | 单位 |
I | 培养基4A-1 | 31.120 | g/L |
NucellinTM | 40.000 | ml/L | |
H/P贮存液 | 20.000 | ml/L | |
亚硒酸盐贮存液 | 2.000 | ml/L | |
PVA | 2.400 | g/L | |
NaH2PO4·H2O | 2.610 | g/L | |
MgSO4·7H2O | 0.430 | g/L | |
天冬氨酸 | 1.330 | g/L | |
谷氨酸 | 0.588 | g/L | |
亚油酸 | 0.840 | ml/L | |
硫辛酸 | 2.100 | ml/L | |
酪氨酸二钠盐(Mw 225) | 1.790 | g/L | |
1000×微量元素B | 6.000 | ml/L | |
葡萄糖 | 100.000 | g/L | |
谷氨酰胺 | 14.600 | g/L | |
pH至7.0 | |||
记录摩尔渗透压浓度 | 1064.000 | mOsm | |
II | 半胱氨酸(400mM) | 加入108ml微量元素D、0.25g FeSO4·7H2O |
至280ml半胱氨酸贮存液 | |||
III | 叶酸 | 720ml 6mM叶酸 |
部分 | 成分 | 含量 | 单位 |
I | 培养基4A-1 | 24.896 | g/L |
NucellinTM | 32.000 | ml/L | |
H/P贮存液 | 16.000 | ml/L | |
亚硒酸盐贮存液 | 1.600 | ml/L | |
PVA | 2.400 | g/L | |
NaH2PO4·H2O | 2.088 | g/L | |
MgSO4·7H2O | 0.344 | g/L | |
天冬氨酸 | 1.064 | g/L |
谷氨酸 | 0.470 | g/L | |
亚油酸 | 0.672 | ml/L | |
硫辛酸 | 1.680 | ml/L | |
酪氨酸二钠盐(Mw 225) | 1.432 | g/L | |
1000×微量元素B | 9.000 | ml/L | |
谷氨酰胺 | 6.280 | g/L | |
pH至7.0 | |||
记录摩尔渗透压浓度 | 295.000 | mOsm | |
II | 半胱氨酸(400mM) | 加入108ml微量元素D、0.25g FeSO4·7H2O至280ml半胱氨酸贮存液 | |
III | 叶酸 | 720ml 6mM叶酸 |
部分 | 成分 | 含量 | 单位 |
I | 培养基4A-1 | 24.896 | g/L |
NucellinTM | 32.000 | ml/L |
H/P贮存液 | 16.000 | ml/L | |
亚硒酸盐贮存液 | 1.600 | ml/L | |
PVA | 2.400 | g/L | |
NaH2PO4·H2O | 2.088 | g/L | |
MgSO4·7H2O | 0.344 | g/L | |
天冬氨酸 | 1.064 | g/L | |
谷氨酸 | 0.470 | g/L | |
亚油酸 | 0.672 | ml/L | |
硫辛酸 | 1.680 | ml/L | |
酪氨酸二钠盐(Mw 225) | 1.432 | g/L | |
1000×微量元素B | 9.000 | ml/L | |
葡萄糖 | 50.000 | g/L | |
谷氨酰胺 | 7.300 | g/L | |
pH至7.0 | |||
FeSO4·7H2O(1mM贮存液) | 15.000 | ml/L | |
记录摩尔渗透压浓度 | 607.000 | mOsm | |
II | 叶酸 | 720ml 6mM叶酸 | |
III | 半胱氨酸(400mM) | 加入108ml微量元素D、0.25g FeSO4·7H2O至280ml半胱氨酸贮存液 |
成分 | 含量 | 单位 |
培养基4A-1 | 24.896 | g/L |
NucellinTM | 32.000 | ml/L |
H/P贮存液 | 16.000 | ml/L |
亚硒酸盐贮存液 | 1.600 | ml/L |
PVA | 2.400 | g/L |
NaH2PO4·H2O | 2.088 | g/L |
MgSO4·7H2O | 0.344 | g/L |
天冬氨酸 | 1.064 | g/L |
谷氨酸 | 0.470 | g/L |
亚油酸 | 0.672 | ml/L |
硫辛酸 | 1.680 | ml/L |
酪氨酸二钠盐(Mw 225) | 1.432 | g/L |
葡萄糖 | 66.700 | g/L |
谷氨酰胺 | 7.300 | g/L |
叶酸 | 70.560 | mg/L |
酸性半胱氨酸(400mM) | 6.250 | ml/L |
FeSO4贮存液(1mM) | 23.000 | ml/L |
1000×微量元素B | 9.000 | ml/L |
1000×微量元素D | 3.300 | ml/L |
预期pH 6.11 | 调为7.0 | |
记录摩尔渗透压浓度 | 724.000 | mOsm |
成分 | 含量 | 单位 |
培养基4A-1 | 18.672 | g/L |
NucellinTM | 24.000 | Ml/L |
H/P贮存液 | 12.000 | Ml/L |
亚硒酸盐贮存液 | 1.200 | Ml/L |
PVA | 2.400 | g/L |
天冬酰胺一水合物 | 1.620 | g/L |
NaH2PO4·H2O | 1.566 | g/L |
MgSO4·7H2O | 0.258 | g/L |
谷氨酰胺 | 5.475 | g/L |
硫胺素 | 0.040 | g/L |
预溶的培养基4B和葡萄糖 | ~175 | ml/L |
酸性半胱氨酸(400mM) | 4.688 | ml/L |
记录pH | ||
用5N盐酸调节pH为7.2 |
FeSO4贮存液(1mM) | 17.250 | ml/L |
1000×微量元素B | 6.750 | ml/L |
1000×微量元素D | 2.475 | ml/L |
记录pH(预计为7.18) | ||
记录Osm | 566.000 | |
预溶的培养基4B和葡萄糖*(用于1L补料培养基) | ||
水 | 150ml | |
将培养基4B(14.5g)与葡萄糖(38.3g)混合 | 加入 | |
用25%NaOH调节pH值直至溶解(pH约为10.25) |
对照方法 | 无谷氨酰胺补料方法 | |
起始培养基谷氨酰胺(mM) | 13mM | 13mM |
谷氨酰胺补料 | 第4天,5mM | 不加谷氨酰胺 |
补料培养基 | 培养基5(含37.5mM谷氨酰胺) | 不含谷氨酰胺的培养基5 |
补料方案 | 从第5天每天4% | 从第5天每天4% |
温度调为31℃ | 第4天 | 第5天 |
对照方法 | 低谷氨酰胺方法 | |
起始培养基谷氨酰胺(mM) | 13mM | 4mM |
谷氨酰胺补料 | 第4天,5mM | 不补加谷氨酰胺 |
补料培养基 | 培养基5(含37.5mM | 不含谷氨酰胺的培养基5 |
谷氨酰胺) | ||
补料方案 | 从第5天起每日4% | 从第5天起每日4% |
温度调为31℃ | 第4天 | 第5天 |
天数 | 9040-44 | 9040-56 | 9040-64 | |||
谷氨酸1 | 谷氨酰胺1 | 谷氨酸2 | 谷氨酰胺2 | 谷氨酸3 | 谷氨酰胺3 | |
0 | ||||||
1 | ||||||
2 | ||||||
3 | ||||||
4 | 5mM gln | 5mM gln | 3g/L gluc3 | 7.7mM gln2.9g/l gluc | ||
5 | 3.6g/ gluc | 5mM gln5.5g/L gluc | 3.5g/L gluc | 5mM gln,6g/L gluc | 3g/L gluc | 3g/L gluc |
6 | 12%16×培养基4 | 12%16×培养基4 | 17%培养基5 | 17%16×培养基4 | 29%培养基5 | 29%培养基5 |
7 | 4mM gln | |||||
8 | ||||||
9 | 2.5g/L gluc | |||||
10 | 10%16×培养基4 | 10%16×培养基4 | 8%培养基5 | 5%16×培养基4 | ||
11 | 1g/L gluc | |||||
12 | ||||||
13 | 1g/L gluc |
氨基酸 | mg/L | mM | 微量元素 | μg/L | nM |
丙氨酸 | 142.48 | 1.60 | 亚硒酸钠 | 40.00 | 231.35 |
精氨酸 | 1528.84 | 8.79 | CuSO4 | 3.44 | 21.51 |
天冬酰胺一水合物 | 1080.60 | 7.20 | CuSO4·5H2O | 7.49 | 30.00 |
天冬氨酸 | 532.40 | 4.00 | FeSO4·7H2O | 2534 | 9115 |
胱氨酸二盐酸盐 | 473.00 | 1.51 | ZnSO4·7H2O | 2704 | 9421 |
谷氨酸 | 235.38 | 1.60 | MnSO4·H2O | 0.17 | 1.01 |
谷氨酰胺 | 4820.00 | 33.01 | Na2SiO3·9H2O | 140 | 492.84 |
甘氨酸 | 120.07 | 1.60 | (NH4)6Mo7O24·4H2O | 1.24 | 1.00 |
组氨酸盐酸盐一水合物 | 588.32 | 2.80 | NH4VO3 | 0.65 | 5.56 |
异亮氨酸 | 944.52 | 7.21 | NiSO4·6H2O | 0.13 | 0.49 |
亮氨酸 | 1360.75 | 10.39 | SnCl2·2H2O | 0.12 | 0.53 |
盐酸赖氨酸 | 1456.80 | 8.00 | AlCl3·6H2O | 1.20 | 4.97 |
甲硫氨酸 | 477.06 | 3.20 | AgNO3 | 0.17 | 1.00 |
苯丙氨酸 | 660.36 | 4.00 | Ba(C2H3O2)2 | 2.55 | 9.98 |
脯氨酸 | 552.31 | 4.80 | KBr | 0.12 | 1.01 |
丝氨酸 | 1264.70 | 12.04 | CdCl2·2.5H2O | 2.28 | 9.99 |
苏氨酸 | 762.02 | 6.40 | CoCl2·6H2O | 2.38 | 10.00 |
色氨酸 | 260.94 | 1.28 | CrCl3 | 0.32 | 2.02 |
二水合二钠酪氨酸 | 832.62 | 3.19 | NaF | 4.20 | 100.02 |
缬氨酸 | 749.21 | 6.40 | GeO2 | 0.53 | 5.07 |
KI | 0.17 | 1.02 | |||
维生素 | mg/L | mM | RbCl | 1.21 | 10.01 |
生物素 | 3.28 | 0.01 | ZrOCl2·8H2O | 3.22 | 9.99 |
泛酸钙 | 36.02 | 0.08 | |||
氯化胆碱 | 143.28 | 1.03 | 其它成分 | μg/L | nM |
叶酸 | 42.43 | 0.10 | 氢化可的松 | 288 | 0.79 |
肌醇 | 201.71 | 1.12 | 二盐酸腐胺 | 8000 | 49.66 |
烟酰胺 | 32.02 | 0.26 | 亚油酸 | 336.25 | 1.20 |
盐酸吡哆醇 | 32.82 | 0.16 | 硫辛酸 | 840.63 | 4.08 |
核黄素 | 3.60 | 0.01 | |||
盐酸硫胺素 | 35.22 | 0.10 | 其它成分 | mg/L | mM |
维生素B12 | 11.21 | 0.01 | D-葡萄糖(右旋糖) | 33005.99 | 183.37 |
PVA | 2400.00 | ||||
无机盐 | mg/L | mM | NucellinTM | 80.00 | |
KH2PO4 | 1635.00 | 12.02 | |||
MgSO4·7H2O | 171.98 | 0.70 |
GluGln | 改良培养基6+43g/L葡萄糖+4.82g/L谷氨酰胺(对照) |
GluGlu | 改良培养基6+43g/L葡萄糖+4.82g/L谷氨酸 |
GluAsp | 改良培养基6+43g/L葡萄糖+4.36g/L天冬酰胺 |
GluGlyGln | 改良培养基6+43g/L葡萄糖+6.71g/L甘氨酰谷氨酰胺 |
GalGlu | 改良培养基6+43g/L半乳糖+4.82g/L谷氨酰胺 |
GalGln | 改良培养基6+43g/L半乳糖+4.82g/L谷氨酸 |
GalAsp | 改良培养基6+43g/L半乳糖+4.36g/L天冬酰胺 |
GalGlyGln | 改良培养基6+43g/L半乳糖+6.71g/L甘氨酰谷氨酰胺 |
Glu | 培养基6+43g/L葡萄糖 |
分子量 | 培养基7 | 培养基8 | 分批培养基 | |
氨基酸 | mM | mM | mM | |
L-丙氨酸 | 89.0 | 1.08 | 2.4 | 0.2 |
L-精氨酸 | 174.0 | 6.84 | 13.2 | 4 |
L-天冬酰胺一水合物 | 150.0 | 4.76 | 21.4 | 7.5 |
L-天冬氨酸 | 133.0 | 2.40 | 6 | 1.65 |
L-半胱氨酸盐酸盐一水合物 | 176.0 | 0.40 | 0 | 0.4 |
L-胱氨酸二盐酸盐 | 313 | 0.95 | 1.875 | 1 |
L-谷氨酸 | 147.0 | 1.08 | 2.4 | 1.08 |
L-谷氨酰胺 | 146.0 | 13.00 | 37.5 | 4 |
甘氨酸 | 75.0 | 1.28 | 2.4 | 1.54 |
L-组氨酸盐酸盐一水合物 | 210.0 | 1.76 | 4.2 | 1.76 |
L-异亮氨酸 | 131.0 | 4.76 | 10.8 | 2.83 |
L-亮氨酸 | 131.0 | 6.52 | 15.6 | 4.7 |
盐酸赖氨酸 | 182.0 | 5.20 | 12 | 5.2 |
L-甲硫氨酸 | 149.0 | 1.96 | 4.8 | 2.6 |
L-苯丙氨酸 | 165.0 | 2.60 | 6 | 2.2 |
L-脯氨酸 | 115.0 | 3.24 | 7.2 | 4.1 |
L-丝氨酸 | 105.0 | 8.60 | 18 | 8.6 |
L-苏氨酸 | 119.0 | 4.32 | 9.6 | 3.2 |
L-色氨酸 | 204.0 | 0.78 | 1.92 | 1.04 |
二水合L-酪氨酸二钠 | 261.0 | 2.16 | 4.8 | 1.75 |
L-缬氨酸 | 117.0 | 4.32 | 9.6 | 4 |
维生素 | uM | uM | uM | |
生物素 | 244.0 | 8.31 | 20.4 | 11 |
D-泛酸钙 | 476.0 | 46.06 | 112.8 | 46.06 |
氯化胆碱 | 139.0 | 632.2 | 1548 | 840 |
叶酸 | 441.0 | 58.8 | 144 | 58.8 |
I-肌醇 | 180.0 | 686 | 1680 | 911 |
烟酰胺 | 122.0 | 161.7 | 396 | 215 |
盐酸吡哆醇 | 206.0 | 88.15 | 240 | 88 |
盐酸吡哆醛 | 203.0 | 10 | 0 | 10 |
核黄素 | 376.0 | 5.37 | 13.2 | 1.1 |
盐酸硫胺素 | 337.0 | 63.7 | 274.7 | 117 |
维生素B12 | 1355.0 | 4.9 | 12 | 7.8 |
无机盐 | ||||
NaCl | 58.5 | 18.8mM | ||
KCl | 74.6 | 4.2mM | 4.19mM | |
CaCl2 | 111 | 1.05mM | 1.05mM | |
亚硒酸钠 | 173 | 27ug/L | 60ug/L | 60ug/L |
NaH2PO4·H2O | 142 | 4.68mM | 11mM | 4.68mM |
Na2HPO4 | 138 | 0.5mM | 0.3986mM | |
MgSO4 | 120 | 1.15mM | 1.05mM | 1.15mM |
MgCl2 | 95 | 0.3mM | 0.3mM | |
FeSO4·7H2O | 278 | 9uM | 24.675uM | 9uM |
Fe(NO3)3·9H2O | 404 | 0.125uM | 0.124um | |
ZnSO4·7H2O | 287 | 9.2uM | 17uM | 9.2um |
CuSO4 | 160 | 0.05uM | 0.074uM | 0.064um |
NaHCO3 | 84 | 23.8mM | 23.8mM | |
其它 | ||||
葡萄糖 | 180 | 16g/L | 38.3g/L | 15g/L |
聚乙烯醇 | 2.56g/L | 2.4g/L | 2.56g/L | |
氢化可的松 | 363 | 0.23mg/L | 0.43mg/L | 0.28mg/L |
二盐酸腐胺 | 161 | 6.4mg/L | 12mg/L | 7.7mg/L |
丙酮酸钠 | 110 | 500uM | 500uM | |
亚油酸 | 280 | 0.81uM | 1.8uM | 0.81uM |
硫辛酸 | 206 | 2.7uM | 6uM | 2.7uM |
NucellinTM | 54mg/L | 120mg/L | 50mg/L | |
1000×微量元素B | 1.5ml/L | 6.75ml/L | 1.5ml/L |
氨基酸 | mg/L | mM | 微量元素 | μg/L | nM |
丙氨酸 | 17.80 | 0.20 | 亚硒酸钠 | 69.16 | 400.00 |
精氨酸 | 696.00 | 4.00 | Fe(NO3)3·9H2O | 50.00 | 123.76 |
天冬酰胺一水合物 | 3000.00 | 20.00 | CuSO4 | 10.24 | 64.00 |
天冬氨酸 | 219.45 | 1.65 | CuSO4·5H2O | 99.88 | 400.00 |
半胱氨酸盐酸盐一水合物 | 70.40 | 0.40 | FeSO4·7H2O | 4170 | 15000 |
二盐酸合半胱氨酸 | 468.75 | 1.50 | ZnSO4·7H2O | 2640 | 9200 |
谷氨酸一钠 | 33.80 | 0.20 | MnSO4·H2O | 33.80 | 200.00 |
谷氨酰胺 | 584.00 | 4.00 | Na2SiO3·9H2O | 284.07 | 1000 |
甘氨酸 | 115.50 | 1.54 | (NH4)6Mo7O24·4H2O | 247.20 | 200.00 |
组氨酸盐酸盐一水合物 | 474.60 | 2.26 | NH4VO3 | 2.34 | 20.00 |
异亮氨酸 | 570.73 | 4.36 | NiSO4·6H2O | 5.26 | 20.00 |
亮氨酸 | 1030.70 | 7.87 | SnCl2·2H2O | 0.90 | 4.00 |
盐酸赖氨酸 | 1401.40 | 7.70 | AlCl3·6H2O | 0.97 | 4.00 |
甲硫氨酸 | 387.40 | 2.60 | KBr | 0.48 | 4.00 |
苯丙氨酸 | 507.00 | 3.07 | CrCl3 | 15.83 | 100.00 |
脯氨酸 | 539.50 | 4.69 | NaF | 0.17 | 4.00 |
丝氨酸 | 1052.00 | 10.02 | GeO2 | 0.42 | 4.00 |
苏氨酸 | 564.80 | 4.75 | KI | 33.20 | 200.00 |
色氨酸 | 274.16 | 1.34 | RbCl | 0.48 | 4.00 |
二水合酪氨酸二钠 | 745.75 | 2.86 | H3BO3 | 12.37 | 200.00 |
缬氨酸 | 749.00 | 6.40 | LiCl | 0.17 | 4.00 |
维生素 | mg/L | mM | 其它成分 | μg/L | nM |
生物素 | 2.68 | 0.01 | 氢化可的松 | 540.00 | 1.49 |
泛酸钙 | 21.92 | 0.05 | 二盐酸腐胺 | 15000 | 93.11 |
氯化胆碱 | 158.46 | 1.14 | 亚油酸 | 290.00 | 1.04 |
叶酸 | 25.93 | 0.06 | 硫辛酸 | 716.00 | 3.48 |
肌醇 | 163.98 | 0.91 | |||
烟酰胺 | 26.23 | 0.22 | 其它成分 | mg/L | mM |
盐酸吡哆醛 | 2.03 | 0.01 | D-葡萄糖(右旋糖) | 15000.00 | 83.33 |
盐酸吡哆醇 | 36.13 | 0.18 | PVA | 2560.00 | |
核黄素 | 2.41 | 0.01 | NucellinTM | 50.00 | |
盐酸硫胺素 | 39.43 | 0.12 | 丙酮酸钠 | 55.00 | 0.50 |
维生素B12 | 21.17 | 0.02 | |||
无机盐 | mg/L | mM | |||
CaCl2 | 116.55 | 1.05 | |||
KCl | 312.90 | 4.19 | |||
Na2HPO4 | 56.60 | 0.40 | |||
NaCl | 1100.00 | 18.80 | |||
NaH2PO4·H2O | 645.84 | 4.68 | |||
MgSO4 | 138.00 | 1.15 | |||
MgCl2 | 28.50 | 0.30 | |||
NaHCO3 | 2000.00 | 23.81 |
1号反应器 | 2号反应器 | 3号反应器 | 4号反应器 | 5号反应器 | 6号反应器 | |
细胞系 | 抗GDF-8 | |||||
培养基 | 分批培养基(培养基9) | |||||
谷氨酰胺水平 | 1mM | 1mM | 1mM | 4mM | 4mM | 4mM |
天冬酰胺水平 | 8mM | 12mM | 20mM | 8mM | 12mM | 20mM |
播种密度(×106/ml) | 0.3至0.35 | |||||
补料培养基 | 培养基5-谷氨酰胺,第7天加5% | |||||
培养天数 | 12 | |||||
温度改变(37-31℃) | 第6天 | 第6天 | 第6天 | 第5天 | 第5天 | 第4天 |
1号反应器 | 2号反应器 | 3号反应器 | 4号反应器 | 5号反应器 | 6号反应器 | |
细胞系 | 抗GDF-8 | |||||
培养基 | 分批培养基(培养基9) | |||||
谷氨酰胺水平 | 4mM | 4mM | 13mM | 13mM | 13mM | 13mM |
天冬酰胺水平 | 20mM | 20mM | 20mM | 12mM | 12mM | 8mM |
播种密度(×106/ml) | 0.3至0.35 | |||||
补料培养基 | 培养基5(不含谷氨酰胺),第7天加5% | |||||
培养天数 | 12 | |||||
温度改变(37-31℃) | 第4天 | 第4天 | 第4天 | 第4天 | 第4天 | 第4天 |
培养基 | Gln(mM) | Asn(mM) | 总Gln(mM) | 总Asn(mM) | 补料 | |
培养基1 | 13 | 5 | 29 | 11 | 培养基5,总30% | 5mM Gln,第4天 |
培养基1 | 4 | 5 | 4 | 11 | 培养基5-Gln,总30% | |
分批培养基(培养基9) | 4 | 20 | 4 | 21 | 培养基5-Gln,5%第7天 | |
培养基1+5mM Asn+0.5mM半胱氨酸 | 13 | 10 | 29 | 16 | 培养基5,总30% | 5mM Gln,第4天 |
分批培养基(培养基9) | 13 | 20 | 29 | 21 | 培养基5,总30% | 5mM Gln,第4天 |
培养基 | Gln(mM) | Asn(mM) | 补料 | 第5天 | 第7天 | 第10天 | 第11天 |
培养基1 | 13 | 5 | 培养基5总30%,5mM Gln,第4天 | 8%培养基5 | 12%培养基5 | 8%培养基5 | |
培养基1+AA | 13 | 15 | 培养基5总30%5mM Gln,第4天 | 8%培养基5 | 12%培养基5 | 8%培养基5 | |
培养基1+Vit,H/P,E,Fe | 13 | 5 | 培养基5总30%,5mM Gln,第4天 | 8%培养基5 | 12%培养基5 | 8%培养基5 | 4g/L葡萄糖 |
培养基1+所有 | 13 | 15 | 培养基5总30%,5mM Gln,第4天 | 8%培养基5 | 12%培养基5 | 8%培养基5 | 4g/L葡萄糖 |
含有13mM谷氨酰胺的培养基9 | 13 | 20 | 培养基5总30%,5mM Gln,第4天 | 8%培养基5 | 12%培养基5 | 8%培养基5 |
微量元素 | μg/L | nM |
(NH4)6Mo7O24·4H2O | 123.60 | 100.00 |
AlCl3·6H2O | 0.48 | 2.00 |
H3BO3 | 6.18 | 100.00 |
CrCl3 | 7.92 | 50.00 |
CuSO4·5H2O | 49.94 | 200.00 |
GeO2 | 0.21 | 2.00 |
KBr | 0.24 | 2.00 |
KI | 16.60 | 100.00 |
LiCl | 0.08 | 2.00 |
MnSO4·H2O | 16.90 | 100.00 |
Na2SiO3·9H2O | 142.03 | 500.00 |
NaF | 0.08 | 2.00 |
NH4VO3 | 1.17 | 10.00 |
NiSO4·6H2O | 2.63 | 10.00 |
RbCl | 0.24 | 2.00 |
SnCl2·2H2O | 0.45 | 2.00 |
亚硒酸钠 | 34.58 | 200.00 |
培养基 | Gln(mM) | Asn(mM) | 补料 | 温度变化 | 第4天 | 第5天 | 第7天 | 第10天 |
培养基1+Fe | 13 | 15 | 培养基5总30% | 第4天 | 5mM Gln,第4天 | 8%培养基5 | 12%培养基5 | 8%培养基5 |
培养基1+E | 13 | 15 | 培养基5总30% | 第4天 | 5mM Gln,第4天 | 8%培养基5 | 12%培养基5 | 8%培养基5 |
培养基1+Vit | 13 | 15 | 培养基5总30% | 第4天 | 5mM Gln,第4天 | 8%培养基5 | 12%培养基5 | 8%培养基5 |
培养基1+Fe+E | 13 | 15 | 培养基5总30% | 第4天 | 5mM Gln,第4天 | 8%培养基5 | 12%培养基5 | 8%培养基5 |
培养基1+Fe+Vit | 13 | 15 | 培养基5总30% | 第4天 | 5mM Gln,第4天 | 8%培养基5 | 12%培养基5 | 8%培养基5 |
培养基1+E+Vit | 13 | 15 | 培养基5总30% | 第4天 | 5mM Gln,第4天 | 8%培养基5 | 12%培养基5 | 8%培养基5 |
含13mM谷氨酰胺的培养基9 | 13 | 20 | 培养基5总30% | 第4天 | 5mM Gln,第4天 | 8%培养基5 | 12%培养基5 | 8%培养基5 |
补料 | |||||||||
培养基 | Asn | Gln | 第3天 | 第4天 | 第5天 | 第6天 | 第7天 | 第10天 | 第11天 |
培养基3 | 14mM | 4mM | 3.3%培养基5-Gln | 3.3%培养基5-Gln | 3.3%培养基5-Gln | 3.3%培养基5-Gln | 10%培养基5-Gln | 3.3%培养基5-Gln | 3.3%培养基5-Gln |
培养基3 | 14mM | 4mM | 3.3%培养基5-Gln | 3.3%培养基5-Gln | 3.3%培养基5-Gln | 3.3%培养基5-Gln | 10%培养基5-Gln | 3.3%培养基5-Gln | 3.3%培养基5-Gln |
培养基1 | 14mM | 4mM | 8%培养基5-Gln | 12%培养基5-Gln | 8%培养基5-Gln | ||||
培养基9 | 20mM | 4mM | 5%培养基5-Gln |
培养基 | Asn | Gln | 补料 | 氨基酸(起始)/(总量) | 离子/总氨基酸 |
3培养基 | 14mM | 4mM | 34%培养基5-Gln | 34mM/64mM | 1.75 |
培养基9 | 20mM | 4mM | 5%培养基5-Gln | 91.4mM/94.3mM | .72 |
培养基1 | 14mM | 4mM | 31.6%培养基5-Gln | 78mM/96.4mM | .74 |
培养基 | Gln(mM) | Asn(mM) | 变温天数 | 补料 | 滴度(ug/ml) | 滴度/1200 | 总Gln | 总Asn | 总Gln+Asn |
培养基9 | 1 | 8 | 6 | 5%培养基5-Gln | 615.2 | 0.51 | 1 | 9 | 10 |
培养基9 | 1 | 8 | 6 | 5%培养基5-Gln | 857.1 | 0.71 | 4 | 9 | 13 |
培养基9 | 1 | 12 | 6 | 5%培养基5-Gln | 947 | 0.79 | 1 | 13 | 14 |
培养基9 | 4 | 12 | 4 | 5%培养基5-Gln | 1184 | 0.99 | 4 | 13 | 17 |
培养基9 | 4 | 20 | 4 | 5%培养基5-Gln | 769.6 | 0.64 | 1 | 21 | 22 |
培养基9 | 4 | 8 | 5 | 5%培养基5-Gln | 1262.6 | 1.05 | 13 | 9 | 22 |
培养基9 | 4 | 20 | 4 | 5%培养基5-Gln | 1198 | 1.00 | 4 | 21 | 25 |
培养基9 | 4 | 20 | 4 | 5%培养基5-Gln | 1321.1 | 1.10 | 4 | 21 | 25 |
培养基9 | 4 | 20 | 4 | 5%培养基5-Gln | 1162.4 | 0.97 | 4 | 21 | 25 |
培养基9 | 13 | 20 | 4 | 5%培养基5-Gln | 1436.6 | 1.20 | 4 | 21 | 25 |
培养基9 | 15 | 12 | 4 | 5%培养基5-Gln | 1638.6 | 1.37 | 13 | 13 | 26 |
培养基9 | 13 | 12 | 4 | 5%培养基5-Gln | 1606.7 | 1.34 | 13 | 13 | 26 |
培养基9 | 13 | 20 | 4 | 5%培养基5-Gln | 1075.91 | 0.90 | 13 | 21 | 34 |
培养基9 | 13 | 20 | 4 | 5%培养基5-Gln | 1058.4 | 0.88 | 13 | 21 | 34 |
培养基9 | 13 | 20 | 4 | 5%培养基5-Gln | 1075.91 | 0.90 | 15 | 21 | 36 |
培养基9 | 13 | 5 | 4 | Asn、Gln、5%培养基5-Gln | 974.52 | 0.81 | 28.5 | 11 | 39.5 |
培养基9 | 13 | 20 | 4 | Asn、Gln、5%培养基5-Gln | 831.81 | 0.69 | 28.5 | 26 | 54.5 |
培养基9 | 13 | 20 | 4 | 第4天,共30%培养基5,5mM Gln | 975.4 | 0.81 | 28.5 | 26 | 54.5 |
培养基9 | 13 | 20 | 4 | 第4天,共30%培养基5,5mM Gln | 973.5 | 0.81 | 28.5 | 26 | 54.5 |
标准化滴度 | Gln>15,Gln<2 | 2<Gln<15 |
平均值 | 0.724649917 | 1.033147493 |
方差 | 0.013326655 | 0.036834109 |
观测数量 | 7 | 12 |
合并方差 | 0.028537361 | |
假设均值差异 | 0 | |
df | 17 | |
t统计量 | -3.839791986 | |
单尾P(T<=t) | 0.000656219 | |
单尾t临界 | 1.739606432 | |
双尾P(T<=t) | 0.001312438 | |
双尾t临界 | 2.109818524 |
标准化滴度 | Asn+Gln>36,Asn+Gln<16 | 16<Asn+Gln<36 |
平均值 | 0.735066584 | 1.027071104 |
方差 | 0.012061148 | 0.041504987 |
观测数量 | 7 | 12 |
合并方差 | 0.031113044 | |
假设均值差异 | 0 | |
df | 17 | |
t统计量 | -3.480816823 | |
单尾P(T<=t) | 0.001430281 | |
单尾t临界 | 1.739606432 |
双尾P(T<=t) | 0.002860561 | |
双尾t临界 | 2.109818524 |
批号 | 描述 | 起始培养基 | 补料量 | Gln补料? | 接种来源 | 接种密度(活细胞/ml) |
1 | 1期 | 培养基10 | 38%* | 是 | 摇摆袋 | 0.2×106 |
2 | 富集培养基,高Gln(1) | 培养基1 | 16% | 是 | 摇摆袋 | 0.2×106 |
3 | 富集培养基,高Gln(2) | 培养基1 | 16% | 是 | 摇摆袋 | 0.2×106 |
4 | 富集培养基,低Gln | 培养基1 | 15% | 否 | 摇摆袋 | 0.2×106 |
5 | 平衡培养基,低Gln(1) | 培养基12 | 10% | 否 | 摇摆袋 | 0.2×106 |
6 | 平衡培养基,低Gln(2) | 培养基12 | 9% | 否 | 摇摆袋 | 0.2×106 |
7 | 平衡培养基,低Gln,密集接种 | 培养基12 | 5% | 否 | 高密度生物反应器 | 2.0×106 |
培养基10 | 培养基11 | 培养基12 | ||||
氨基酸 | mg/L | mM | mg/L | mM | mg/L | mM |
丙氨酸 | 24.87 | 0.28 | 142.48 | 1.60 | 17.80 | 0.20 |
精氨酸 | 423.43 | 2.43 | 1528.84 | 8.79 | 696.00 | 4.00 |
天冬酰胺一水合物 | 173.90 | 1.16 | 1080.60 | 7.20 | 1500.00 | 10.00 |
天冬氨酸 | 52.72 | 0.40 | 532.40 | 4.00 | 219.45 | 1.65 |
半胱氨酸盐酸盐一水合物 | 70.01 | 0.40 | 70.40 | 0.40 | ||
半胱氨酸二盐酸盐 | 62.09 | 0.20 | 470.00 | 1.50 | 312.50 | 1.00 |
谷氨酸 | 41.08 | 0.28 | 235.38 | 1.60 | ||
谷氨酸一钠 | 33.80 | 0.20 | ||||
谷氨酰胺 | 1162.40 | 7.96 | 6000.00 | 41.10 | 584.00 | 4.00 |
甘氨酸 | 35.92 | 0.48 | 120.07 | 1.60 | 115.50 | 1.54 |
组氨酸盐酸盐一水合物 | 75.27 | 0.36 | 588.32 | 2.80 | 369.60 | 1.76 |
异亮氨酸 | 151.90 | 1.16 | 944.52 | 7.21 | 370.73 | 2.83 |
亮氨酸 | 172.69 | 1.32 | 1360.75 | 10.39 | 615.70 | 4.70 |
盐酸赖氨酸 | 218.38 | 1.20 | 1456.80 | 8.00 | 946.40 | 5.20 |
甲硫氨酸 | 53.55 | 0.36 | 477.06 | 3.20 | 387.40 | 2.60 |
苯丙氨酸 | 98.81 | 0.60 | 660.36 | 4.00 | 363.00 | 2.20 |
脯氨酸 | 96.40 | 0.84 | 552.31 | 4.80 | 471.50 | 4.10 |
丝氨酸 | 273.07 | 2.60 | 1264.70 | 12.04 | 903.00 | 8.60 |
苏氨酸 | 132.81 | 1.12 | 762.02 | 6.40 | 380.80 | 3.20 |
色氨酸 | 28.99 | 0.14 | 260.94 | 1.28 | 212.16 | 1.04 |
二水合酪氨酸二钠 | 145.10 | 0.56 | 832.62 | 3.19 | 456.75 | 1.75 |
缬氨酸 | 131.17 | 1.12 | 749.21 | 6.40 | 468.00 | 4.00 |
维生素 | mg/L | μM | mg/L | μM | mg/L | μM |
生物素 | 0.36 | 1.49 | 3.28 | 13.45 | 2.68 | 11.00 |
泛酸钙 | 4.03 | 8.47 | 36.02 | 75.67 | 21.93 | 46.06 |
氯化胆碱 | 16.11 | 115.92 | 143.28 | 1030 | 116.76 | 840.00 |
叶酸 | 4.76 | 10.80 | 42.43 | 96.22 | 25.93 | 58.80 |
肌醇 | 22.64 | 125.79 | 201.71 | 1120 | 163.98 | 911.00 |
烟酰胺 | 3.61 | 29.62 | 32.02 | 262.44 | 26.23 | 215.00 |
盐酸吡哆醛 | 1.99 | 9.83 | 2.03 | 10.00 | ||
盐酸吡哆醇 | 1.67 | 8.10 | 32.82 | 159.31 | 18.13 | 88.00 |
核黄素 | 0.40 | 1.06 | 3.60 | 9.58 | 0.41 | 1.10 |
盐酸硫胺素 | 3.92 | 11.64 | 35.22 | 104.51 | 39.43 | 117.00 |
维生素B12 | 1.34 | 0.99 | 11.21 | 8.27 | 10.57 | 7.80 |
无机盐 | mg/L | mM | mg/L | mM | mg/L | mM |
CaCl2 | 115.78 | 1.04 | 113.27 | 1.02 | 116.55 | 1.05 |
KCl | 310.94 | 4.17 | 312.90 | 4.19 |
KH2PO4 | 1640.00 | 12.06 | ||||
Na2HPO4 | 70.81 | 0.50 | 56.60 | 0.40 | ||
NaCl | 3704.96 | 63.44 | ||||
NaH2PO4·H2O | 114.53 | 0.83 | 645.84 | 4.68 | ||
MgSO4 | 48.70 | 0.41 | 138.00 | 1.15 | ||
MgSO4·7H2O | 8.60 | 0.03 | 170.00 | 0.69 | ||
MgCl2 | 28.53 | 0.30 | 28.50 | 0.30 | ||
NaHCO3 | 1220.00 | 14.52 | 2000.00 | 23.81 | ||
微量元素 | μg/L | nM | μg/L | nM | μg/L | nM |
亚硒酸钠 | 7.00 | 40.49 | 40.00 | 231.35 | 53.65 | 310.27 |
Fe(NO3)3·9H2O | 49.86 | 123.42 | 50.00 | 123.76 | ||
CuSO4 | 0.97 | 6.06 | 3.44 | 21.51 | 10.00 | 62.50 |
CuSO4·5H2O | 7.49 | 30.00 | 7.49 | 30.00 | 49.94 | 200.00 |
FeSO4·7H2O | 1542 | 5549 | 2534 | 9115 | 3366 | 12000 |
ZnSO4·7H2O | 1383 | 4821 | 2704 | 9421 | 2640 | 9198 |
MnSO4·H2O | 0.17 | 1.01 | 0.17 | 1.01 | 16.90 | 100.00 |
Na2SiO3·9H2O | 140 | 492.84 | 140.00 | 492.84 | 142.03 | 500.00 |
(NH4)6Mo7O24·4H2O | 1.24 | 1.00 | 1.24 | 1.00 | 123.60 | 100.00 |
NH4VO3 | 0.65 | 5.56 | 0.65 | 5.56 | 1.17 | 10.00 |
NiSO4·6H2O | 0.13 | 0.49 | 0.13 | 0.49 | 2.63 | 10.00 |
SnCl2·2H2O | 0.12 | 0.53 | 0.12 | 0.53 | 0.45 | 2.00 |
AlCl3·6H2O | 1.20 | 4.97 | 0.48 | 2.00 | ||
AgNO3 | 0.17 | 1.00 | ||||
Ba(C2H3O2)2 | 2.55 | 9.98 | ||||
KBr | .12 | 1.01 | 0.24 | 2.00 | ||
CdCl2·2.5H2O | 2.28 | 9.99 |
CoCl2·6H2O | 2.38 | 10.00 | ||||
CrCl3 | 0.32 | 2.02 | 7.92 | 50.00 | ||
NaF | 4.20 | 100.02 | 0.08 | 2.00 | ||
GeO2 | 0.53 | 5.07 | 0.21 | 2.00 | ||
KI | 0.17 | 1.02 | 16.60 | 100 | ||
RbCl | 1.21 | 10.01 | 0.24 | 2.00 | ||
ZrOCl2·8H2O | 3.22 | 9.99 | ||||
H3BO3 | 6.18 | 100.00 | ||||
LiCl | 0.08 | 2.00 | ||||
其它成分 | μg/L | μM | μg/L | μM | μg/L | μM |
氢化可的松 | 86.40 | .24 | 288.00 | 0.79 | 360.00 | 0.99 |
二盐酸腐胺 | 2480 | 15.39 | 8000 | 49.66 | 10000 | 62.07 |
亚油酸 | 56.69 | 0.20 | 336.25 | 1.20 | 290.00 | 1.04 |
硫辛酸 | 141.71 | 0.69 | 840.63 | 4.08 | 716.00 | 3.48 |
其它成分 | mg/L | mM | mg/L | mM | mg/L | mM |
D-葡萄糖(右旋糖) | 11042.24 | 61.35 | 43005.99 | 238.92 | 15000.00 | 83.33 |
PVA | 2520.00 | 2400.00 | 2560.00 | |||
NucellinTM | 14.00 | 80.00 | 50.00 | |||
丙酮酸钠 | 54.85 | 0.50 | 55.00 | 0.50 |
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Cited By (5)
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CN105567627A (zh) * | 2007-04-26 | 2016-05-11 | 中外制药株式会社 | 使用含高浓度氨基酸的培养基的细胞培养方法 |
CN105567627B (zh) * | 2007-04-26 | 2020-09-29 | 中外制药株式会社 | 使用含高浓度氨基酸的培养基的细胞培养方法 |
CN107988166A (zh) * | 2011-07-01 | 2018-05-04 | 安姆根有限公司 | 哺乳动物细胞培养 |
CN110382687A (zh) * | 2017-03-09 | 2019-10-25 | 赢创德固赛有限公司 | 包含二肽的培养基 |
CN110382687B (zh) * | 2017-03-09 | 2023-05-09 | 赢创运营有限公司 | 包含二肽的培养基 |
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