CL2011003162A1 - Celula huesped recombinante de yarrowia sp. que produce un aceite que comprende al menos 50% en peso de acido eicosapentaenoico (epa) y tiene una relacion epa: acido linoleico de al menos 3,1:1; y metodo para elaborar un aceite microbiano que comprende epa. - Google Patents

Celula huesped recombinante de yarrowia sp. que produce un aceite que comprende al menos 50% en peso de acido eicosapentaenoico (epa) y tiene una relacion epa: acido linoleico de al menos 3,1:1; y metodo para elaborar un aceite microbiano que comprende epa.

Info

Publication number
CL2011003162A1
CL2011003162A1 CL2011003162A CL2011003162A CL2011003162A1 CL 2011003162 A1 CL2011003162 A1 CL 2011003162A1 CL 2011003162 A CL2011003162 A CL 2011003162A CL 2011003162 A CL2011003162 A CL 2011003162A CL 2011003162 A1 CL2011003162 A1 CL 2011003162A1
Authority
CL
Chile
Prior art keywords
epa
delta
oil
desatures
tfa
Prior art date
Application number
CL2011003162A
Other languages
English (en)
Spanish (es)
Inventor
Seung-Pyo Hong
Pamela L Sharpe
Zhixiong Xue
Narendra S Yadav
Qun Zhu
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of CL2011003162A1 publication Critical patent/CL2011003162A1/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436Fatty acid esters
    • C12P7/6445Glycerides
    • C12P7/6472Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436Fatty acid esters
    • C12P7/6445Glycerides
    • C12P7/6458Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CL2011003162A 2009-06-16 2011-12-15 Celula huesped recombinante de yarrowia sp. que produce un aceite que comprende al menos 50% en peso de acido eicosapentaenoico (epa) y tiene una relacion epa: acido linoleico de al menos 3,1:1; y metodo para elaborar un aceite microbiano que comprende epa. CL2011003162A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US18736609P 2009-06-16 2009-06-16

Publications (1)

Publication Number Publication Date
CL2011003162A1 true CL2011003162A1 (es) 2012-07-20

Family

ID=43356704

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2011003162A CL2011003162A1 (es) 2009-06-16 2011-12-15 Celula huesped recombinante de yarrowia sp. que produce un aceite que comprende al menos 50% en peso de acido eicosapentaenoico (epa) y tiene una relacion epa: acido linoleico de al menos 3,1:1; y metodo para elaborar un aceite microbiano que comprende epa.

Country Status (9)

Country Link
EP (1) EP2475679B1 (enExample)
JP (1) JP5893555B2 (enExample)
CN (1) CN102803289B (enExample)
AU (1) AU2010260231B2 (enExample)
BR (1) BRPI1009649A2 (enExample)
CA (1) CA2761589C (enExample)
CL (1) CL2011003162A1 (enExample)
DK (1) DK2475679T3 (enExample)
WO (1) WO2010147904A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120040076A1 (en) * 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company Aquaculture feed compositions
WO2013192007A1 (en) * 2012-06-19 2013-12-27 E. I. Du Pont De Nemours And Company MUTANT ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES
CN108330114B (zh) * 2018-03-21 2020-11-03 北京大学 一种利用epa的甘油二酯酰基转移酶及其应用
WO2020082221A1 (zh) * 2018-10-23 2020-04-30 利时雨 重组磷酸胆碱胞苷转移酶的粘红酵母携带外源多肽的活细胞脂质体及其应用
CN117568177A (zh) * 2023-11-16 2024-02-20 鲁东大学 一种解脂耶氏酵母提取物的制备方法及应用

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4670285A (en) 1982-08-06 1987-06-02 The University Of Toronto Innovations Foundation Infant formula
US5648564A (en) 1995-12-21 1997-07-15 Kemin Industries, Inc. Process for the formation, isolation and purification of comestible xanthophyll crystals from plants
US6797303B2 (en) 2001-09-04 2004-09-28 Lycored Natural Products Industries Ltd. Carotenoid extraction process
ATE517984T1 (de) * 2003-02-27 2011-08-15 Basf Plant Science Gmbh Verfahren zur herstellung mehrfach ungesättigter fettsäuren
EP3121269A1 (de) 2003-03-31 2017-01-25 University Of Bristol Neue pflanzliche acyltransferase spezifisch für langkettige mehrfach ungesättigte fettsäuren
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7259255B2 (en) 2003-06-25 2007-08-21 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US7504259B2 (en) 2003-11-12 2009-03-17 E. I. Du Pont De Nemours And Company Δ12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast
CN101018862B (zh) * 2004-04-22 2013-10-09 联邦科学技术研究组织 用重组细胞合成长链多不饱和脂肪酸
US7550651B2 (en) 2004-06-25 2009-06-23 E.I. Du Pont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
US7550286B2 (en) * 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
DE102004062294A1 (de) 2004-12-23 2006-07-06 Basf Plant Science Gmbh Verfahren zur Herstellung von mehrfach ungesättigten langkettigen Fettsäuren in transgenen Organismen
US7772444B2 (en) * 2005-05-19 2010-08-10 E. I. Du Pont De Nemours And Company Method for the production of resveratrol in a recombinant oleaginous microorganism
US7470532B2 (en) 2005-10-19 2008-12-30 E.I. Du Pont De Nemours And Company Mortierella alpina C16/18 fatty acid elongase
WO2007061742A1 (en) 2005-11-23 2007-05-31 E. I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
WO2008054565A1 (en) 2006-10-30 2008-05-08 E. I. Du Pont De Nemours And Company Δ17 desaturase and its use in making polyunsaturated fatty acids
US7709239B2 (en) 2006-12-07 2010-05-04 E.I. Du Pont De Nemours And Company Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
US8846374B2 (en) 2006-12-12 2014-09-30 E I Du Pont De Nemours And Company Carotenoid production in a recombinant oleaginous yeast
DK2129777T3 (en) * 2007-04-03 2016-01-25 Du Pont MULTIZYMER AND USE THEREOF FOR THE PRODUCTION OF polyunsaturated fatty acids
WO2009001315A2 (en) 2007-06-26 2008-12-31 Staahl Ulf Use of a class of genes encoding lysophospholipid acyl transferases for application in agriculture, biotechnology, and medicine
RU2496881C2 (ru) 2007-07-23 2013-10-27 Сантори Холдингз Лимитед Композиции жирных кислот, имеющие новую пропорцию жирных кислот
CA2701130A1 (en) 2007-10-03 2009-04-09 E.I. Du Pont De Nemours And Company Peroxisome biogenesis factor protein (pex) disruptions for altering the content of polyunsaturated fatty acids and the total lipid content in oleaginous eukaryotic organisms
US8323935B2 (en) 2007-10-03 2012-12-04 E I Du Pont De Nemours And Company Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production
CA2744716C (en) 2008-12-18 2017-05-23 E.I. Du Pont De Nemours And Company Reducing byproduction of malonates in a fermentation process

Also Published As

Publication number Publication date
WO2010147904A1 (en) 2010-12-23
DK2475679T3 (da) 2016-05-09
CN102803289B (zh) 2015-07-22
AU2010260231A1 (en) 2011-11-10
EP2475679A4 (en) 2013-04-03
CN102803289A (zh) 2012-11-28
BRPI1009649A2 (pt) 2016-03-15
CA2761589C (en) 2017-12-19
CA2761589A1 (en) 2010-12-23
JP5893555B2 (ja) 2016-03-23
JP2012529913A (ja) 2012-11-29
AU2010260231B2 (en) 2017-03-02
EP2475679B1 (en) 2016-02-10
AU2010260231A8 (en) 2011-11-17
EP2475679A1 (en) 2012-07-18

Similar Documents

Publication Publication Date Title
CO6400156A2 (es) Producciòn de aceites específicos en microorganismos heterotrofos
MX339639B (es) Aceites especificos producidos a partir de microorganismos heterotrofos recombinantes.
Ma et al. Physiological and biochemical changes reveal stress-associated photosynthetic carbon partitioning into triacylglycerol in the oleaginous marine alga Nannochloropsis oculata
CL2009001061A1 (es) Microorganismo thraustochytrio aislado que produce acidos grasos que comprenden al menos 40% en peso de acido docosahexaenoico y 0,5% a 6% en peso de ácido n-6 docosapentaenoico en la fraccion de triglicéridos; biomasa y cultivo que comprende dicho microorganismo; aceite microbiano y producto alimenticio; uso del microorganismo en un metodo para producir aceite microbiano, util para tratar la inflamacion.
BR112013004351A2 (pt) célula hospedeira microbiana recombinante e método para a produção de um óleo microbiano compreendendo ácido eicosapentaenócio.
Shen et al. Biosynthesis of high yield fatty acids from Chlorella vulgaris NIES-227 under nitrogen starvation stress during heterotrophic cultivation
MX344012B (es) Aceites adaptados producidos a partir de microorganismos oleaginosos recombinantes.
CL2019002477A1 (es) Producción de ácidos graso poliinsaturados de cadena larga en células vegetales. (divisional solicitud 201403402)
PH12019502112A1 (en) Processes for producing industrial products from plant lipids
Hu et al. The influence of cultivation period on growth and biodiesel properties of microalga Nannochloropsis gaditana 1049
Cheng et al. Statistical optimization of culture media for growth and lipid production of Chlorella protothecoides UTEX 250
CL2011003162A1 (es) Celula huesped recombinante de yarrowia sp. que produce un aceite que comprende al menos 50% en peso de acido eicosapentaenoico (epa) y tiene una relacion epa: acido linoleico de al menos 3,1:1; y metodo para elaborar un aceite microbiano que comprende epa.
CL2019002663A1 (es) Producción de ácidos grasos poliinsaturados de cadena larga en células vegetales. (divisional solicitud 201702448)
BR112013011039A8 (pt) Óleos microbianos com pontos de escorrimento reduzidos, fluidos dielétricos produzidos dos mesmos, e métodos relacionados
BR112018005976A2 (pt) óleos de triglicerídeo tendo moléculas de triglicerídeo assimétricas
EP2649887A3 (en) High eicosapentaenoic acid producing strains of Yarrowia lipolytica
BR112012025585A2 (pt) método e sistemas para produzir biocombustível
BR112013020346A2 (pt) concentrado de ácido eicosapentaenoico, produto farmac~eutico, método de elaboração de um concentrado de ácido eicosapentaenoico e uso de óleo microbiano
EA201400151A1 (ru) Микроорганизмы, продуцирующие эйкозапентаеновую кислоту, композиции жирных кислот, способы их получения и применения
WO2016044779A3 (en) Acyl-acp thioesterases and mutants thereof
PE20180456A1 (es) Acidos grasos poliinsaturados de cadena muy larga a partir de aceites naturales
CL2011003161A1 (es) Celula huesped microbiana oleaginosa recombinante para la produccion mejorada de acido eicosapentaenoico, la que codifica un polipeptido con actividad acil coa lisofosfolipido, metodo para elaborar aceite que comprende dicho acido graso de cadena larga.
JP2010539985A5 (enExample)
CL2011003163A1 (es) Celula huesped microbiana oleaginosa recombinante para la produccion mejorada de al menos un acido graso poliinsaturado de cadena larga que codifica un polipeptido con actividad acilcoa: lisofosfolipido; metodo para elaborar aceite que comprende acido eicosapentaenoico y/o acido docosahexaenoico.
BR112015014781A2 (pt) célula microbiana recombinante e método de produção de um óleo microbiano