CL2011003162A1 - Celula huesped recombinante de yarrowia sp. que produce un aceite que comprende al menos 50% en peso de acido eicosapentaenoico (epa) y tiene una relacion epa: acido linoleico de al menos 3,1:1; y metodo para elaborar un aceite microbiano que comprende epa. - Google Patents

Celula huesped recombinante de yarrowia sp. que produce un aceite que comprende al menos 50% en peso de acido eicosapentaenoico (epa) y tiene una relacion epa: acido linoleico de al menos 3,1:1; y metodo para elaborar un aceite microbiano que comprende epa.

Info

Publication number
CL2011003162A1
CL2011003162A1 CL2011003162A CL2011003162A CL2011003162A1 CL 2011003162 A1 CL2011003162 A1 CL 2011003162A1 CL 2011003162 A CL2011003162 A CL 2011003162A CL 2011003162 A CL2011003162 A CL 2011003162A CL 2011003162 A1 CL2011003162 A1 CL 2011003162A1
Authority
CL
Chile
Prior art keywords
epa
delta
oil
desatures
tfa
Prior art date
Application number
CL2011003162A
Other languages
English (en)
Inventor
Seung-Pyo Hong
Pamela L Sharpe
Zhixiong Xue
Narendra S Yadav
Qun Zhu
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of CL2011003162A1 publication Critical patent/CL2011003162A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

SE DESCRIBE CEPAS MODIFICADAS DE LA LEVADURA OLEAGINOSA YARROWIA LIPOLYTICA CON LA CAPACIDAD DE PRODUCIR UN ACEITE QUE COMPRENDE MÁS DEL 50 POR CIENTO EN PESO DE ÁCIDO EICOSAPENTAENOICO ["EPA"], UN ÁCIDO GRASO POLIINSATURADO W-3, QUE SE DETERMINA COMO UN PESO PORCENTUAL DEL TOTAL DE ÁCIDOS GRASOS ["% DEL TFA"] Y QUE TIENE UNA RELACIÓN DE POR LO MENOS 3.1 DE % DE EPA DEL TFA Y ÁCIDO LINOLEICO DETERMINADO COMO % DEL TFA. ESTAS CEPAS SOBREEXPRESAN POR LO MENOS UNA MULTIENZIMA DELTA 9  ELONGASA/ DELTA 8 DESATURASA, ADEMÁS DE OTRAS DELTA 9 ELONGASAS, DELTA 8 DESATURASAS,DELTA 5 DESATURASAS, DELTA 17 DESATURASAS,DELTA 12 DESATURASAS, C16/18 ELONGASAS HETERÓLOGAS Y, OPCIONALMENTE, SOBREEXPRESAN DIACILGLICEROL COLINOFOSFOTRANSFERASA, MALONIL COA SINTETASA Y/O ACIL-COA LISOFOSFOLIPIDO ACILTRANSFERASA. SE REGULA LA BAJA LA EXPRESIÓN DE POR LO MENOS UNA PROTEÍNA DEL FACTOR DE BIOGÉNESIS DE PEROXISOMAS. SE REIVINDICA LOS MÉTODOS PARA PRODUCIR EPA DENTRO DE LAS CÉLULAS HUÉSPED, LOS ACEITES OBTENIDOS DE LAS CÉLULAS Y LOS PRODUCTOS DE ESTOS.
CL2011003162A 2009-06-16 2011-12-15 Celula huesped recombinante de yarrowia sp. que produce un aceite que comprende al menos 50% en peso de acido eicosapentaenoico (epa) y tiene una relacion epa: acido linoleico de al menos 3,1:1; y metodo para elaborar un aceite microbiano que comprende epa. CL2011003162A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US18736609P 2009-06-16 2009-06-16

Publications (1)

Publication Number Publication Date
CL2011003162A1 true CL2011003162A1 (es) 2012-07-20

Family

ID=43356704

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2011003162A CL2011003162A1 (es) 2009-06-16 2011-12-15 Celula huesped recombinante de yarrowia sp. que produce un aceite que comprende al menos 50% en peso de acido eicosapentaenoico (epa) y tiene una relacion epa: acido linoleico de al menos 3,1:1; y metodo para elaborar un aceite microbiano que comprende epa.

Country Status (9)

Country Link
EP (1) EP2475679B1 (es)
JP (1) JP5893555B2 (es)
CN (1) CN102803289B (es)
AU (1) AU2010260231B2 (es)
BR (1) BRPI1009649A2 (es)
CA (1) CA2761589C (es)
CL (1) CL2011003162A1 (es)
DK (1) DK2475679T3 (es)
WO (1) WO2010147904A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120040076A1 (en) * 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company Aquaculture feed compositions
EP2861728B1 (en) * 2012-06-19 2017-12-27 E. I. du Pont de Nemours and Company Improved production of polyunsaturated fatty acids by coexpression of acyl-coa: lysophosphatidylcholine acyltransferases and phospholipid: diacylglycerol acyltransferases
TWI571510B (zh) * 2016-06-23 2017-02-21 行政院原子能委員會核能研究所 酵母菌菌株的馴化方法及其馴化酵母菌菌株
CN108330114B (zh) * 2018-03-21 2020-11-03 北京大学 一种利用epa的甘油二酯酰基转移酶及其应用
WO2020082221A1 (zh) * 2018-10-23 2020-04-30 利时雨 重组磷酸胆碱胞苷转移酶的粘红酵母携带外源多肽的活细胞脂质体及其应用

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US4670285A (en) 1982-08-06 1987-06-02 The University Of Toronto Innovations Foundation Infant formula
US5648564A (en) 1995-12-21 1997-07-15 Kemin Industries, Inc. Process for the formation, isolation and purification of comestible xanthophyll crystals from plants
US6797303B2 (en) 2001-09-04 2004-09-28 Lycored Natural Products Industries Ltd. Carotenoid extraction process
CA2517253C (en) * 2003-02-27 2018-07-03 Basf Plant Science Gmbh Method for the production of polyunsaturated fatty acids
EP2365063B1 (de) 2003-03-31 2015-10-14 University Of Bristol Neue pflanzliche Acyltransferase spezifisch für langkettige mehrfach ungesättigte Fettsäuren
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7259255B2 (en) 2003-06-25 2007-08-21 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US7504259B2 (en) 2003-11-12 2009-03-17 E. I. Du Pont De Nemours And Company Δ12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast
CN101018862B (zh) * 2004-04-22 2013-10-09 联邦科学技术研究组织 用重组细胞合成长链多不饱和脂肪酸
WO2006012325A1 (en) 2004-06-25 2006-02-02 E.I. Dupont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
US7550286B2 (en) * 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
DE102004062294A1 (de) 2004-12-23 2006-07-06 Basf Plant Science Gmbh Verfahren zur Herstellung von mehrfach ungesättigten langkettigen Fettsäuren in transgenen Organismen
WO2006125000A2 (en) * 2005-05-19 2006-11-23 E.I. Du Pont De Nemours And Company Method for the production of resveratrol in a recombinant oleaginous microorganism
US7470532B2 (en) 2005-10-19 2008-12-30 E.I. Du Pont De Nemours And Company Mortierella alpina C16/18 fatty acid elongase
KR20080071190A (ko) 2005-11-23 2008-08-01 이 아이 듀폰 디 네모아 앤드 캄파니 델타-９ 일롱가제, 및 다중불포화 지방산 생성에 있어서의이들의 용도
US7556949B2 (en) 2006-10-30 2009-07-07 E.I. Du Pont De Nemours And Company Δ17 desaturase and its use in making polyunsaturated fatty acids
US7709239B2 (en) 2006-12-07 2010-05-04 E.I. Du Pont De Nemours And Company Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
US8846374B2 (en) 2006-12-12 2014-09-30 E I Du Pont De Nemours And Company Carotenoid production in a recombinant oleaginous yeast
RS20090413A (en) * 2007-04-03 2010-06-30 E. I. Du Pont De Nemours And Company Multizymes and their use in making polyunsaturated fatty acids
WO2009001315A2 (en) 2007-06-26 2008-12-31 Staahl Ulf Use of a class of genes encoding lysophospholipid acyl transferases for application in agriculture, biotechnology, and medicine
WO2009014140A1 (ja) 2007-07-23 2009-01-29 Suntory Holdings Limited 新規な脂肪酸組成を有する脂肪酸組成物
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CN101883843B (zh) * 2007-10-03 2016-03-02 纳幕尔杜邦公司 破坏过氧化物酶体生物合成因子蛋白(pex)以改变含油真核生物中多不饱和脂肪酸和总脂质含量
EP2358862B1 (en) 2008-12-18 2017-08-02 E. I. du Pont de Nemours and Company Reducing byproduction of malonates in a fermentation process

Also Published As

Publication number Publication date
CN102803289B (zh) 2015-07-22
AU2010260231B2 (en) 2017-03-02
CN102803289A (zh) 2012-11-28
JP5893555B2 (ja) 2016-03-23
AU2010260231A8 (en) 2011-11-17
WO2010147904A1 (en) 2010-12-23
CA2761589A1 (en) 2010-12-23
JP2012529913A (ja) 2012-11-29
AU2010260231A1 (en) 2011-11-10
DK2475679T3 (da) 2016-05-09
BRPI1009649A2 (pt) 2016-03-15
EP2475679A4 (en) 2013-04-03
EP2475679A1 (en) 2012-07-18
EP2475679B1 (en) 2016-02-10
CA2761589C (en) 2017-12-19

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