BR112015014781A2 - célula microbiana recombinante e método de produção de um óleo microbiano - Google Patents
célula microbiana recombinante e método de produção de um óleo microbianoInfo
- Publication number
- BR112015014781A2 BR112015014781A2 BR112015014781A BR112015014781A BR112015014781A2 BR 112015014781 A2 BR112015014781 A2 BR 112015014781A2 BR 112015014781 A BR112015014781 A BR 112015014781A BR 112015014781 A BR112015014781 A BR 112015014781A BR 112015014781 A2 BR112015014781 A2 BR 112015014781A2
- Authority
- BR
- Brazil
- Prior art keywords
- acyltransferase
- cells
- coa
- recombinant microbial
- oil
- Prior art date
Links
- 230000000813 microbial effect Effects 0.000 title abstract 6
- 239000003921 oil Substances 0.000 abstract 3
- 102000040430 polynucleotide Human genes 0.000 abstract 3
- 108091033319 polynucleotide Proteins 0.000 abstract 3
- 239000002157 polynucleotide Substances 0.000 abstract 3
- 102100038805 Lysophospholipid acyltransferase 2 Human genes 0.000 abstract 2
- 235000020673 eicosapentaenoic acid Nutrition 0.000 abstract 2
- HOBAELRKJCKHQD-UHFFFAOYSA-N (8Z,11Z,14Z)-8,11,14-eicosatrienoic acid Natural products CCCCCC=CCC=CCC=CCCCCCCC(O)=O HOBAELRKJCKHQD-UHFFFAOYSA-N 0.000 abstract 1
- 108010052187 1-Acylglycerophosphocholine O-Acyltransferase Proteins 0.000 abstract 1
- 108010054662 2-acylglycerophosphate acyltransferase Proteins 0.000 abstract 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 abstract 1
- 235000021298 Dihomo-γ-linolenic acid Nutrition 0.000 abstract 1
- 102100026665 Malonate-CoA ligase ACSF3, mitochondrial Human genes 0.000 abstract 1
- 108030002650 Phospholipid:diacylglycerol acyltransferases Proteins 0.000 abstract 1
- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 108010022240 delta-8 fatty acid desaturase Proteins 0.000 abstract 1
- HOBAELRKJCKHQD-QNEBEIHSSA-N dihomo-γ-linolenic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/CCCCCCC(O)=O HOBAELRKJCKHQD-QNEBEIHSSA-N 0.000 abstract 1
- 230000003828 downregulation Effects 0.000 abstract 1
- 229960005135 eicosapentaenoic acid Drugs 0.000 abstract 1
- JAZBEHYOTPTENJ-UHFFFAOYSA-N eicosapentaenoic acid Natural products CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O JAZBEHYOTPTENJ-UHFFFAOYSA-N 0.000 abstract 1
- 108010089734 malonyl-CoA synthetase Proteins 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 230000035772 mutation Effects 0.000 abstract 1
- 235000020660 omega-3 fatty acid Nutrition 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 239000000600 sorbitol Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
- C12P7/6432—Eicosapentaenoic acids [EPA]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
- C12P7/6431—Linoleic acids [18:2[n-6]]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01023—1-Acylglycerophosphocholine O-acyltransferase (2.3.1.23), i.e. lysophosphatidylcholine acyltransferase or LPCAT
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
resumo célula microbiana recombinante e método de produção de um óleo microbiano células microbianas recombinantes são divulgadas que produzem um óleo que compreende pelo menos 28% de ácido eicosapentaenoico (epa) medido como uma porcentagem em peso do peso de célula seca. essas células podem compreender uma sequência polinucleotídica que codifica uma acil-coa:lisofosfatidilcolina aciltransferase (lpcat) que compreende pelo menos uma mutação de aminoácido em uma porção da o-aciltransferase ligada à membrana. além disso, as células podem compreender a infrarregulação de uma sequência polinucleotídica endógena que codifica a proteína de utilização do sorbitol sou2 e/ou um ou mais polinucleotídeos que codificam a fosfolipídio:diacilglicerol aciltransferase (pdat), a delta-12 dessaturase, uma multienzima do ácido di-homo-gama-linolênico sintase (dgla), a delta-8 dessaturase, malonil-coa sintetase (mcs) ou acil-coa:ácido lisofosfatídico aciltransferase (lpaat). também são divulgados métodos de uso das células microbianas recombinantes para produzir óleos contendo ácidos graxos ômega-3 poli-insaturados, como o epa.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261740502P | 2012-12-21 | 2012-12-21 | |
PCT/US2013/075896 WO2014100062A1 (en) | 2012-12-21 | 2013-12-18 | Recombinant microbial cells that produce at least 28% eicosapentaenoic acid as dry cell weight |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112015014781A2 true BR112015014781A2 (pt) | 2017-10-10 |
Family
ID=49956389
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112015014781A BR112015014781A2 (pt) | 2012-12-21 | 2013-12-18 | célula microbiana recombinante e método de produção de um óleo microbiano |
Country Status (9)
Country | Link |
---|---|
US (1) | US9701989B2 (pt) |
EP (1) | EP2935601B1 (pt) |
JP (1) | JP2016502852A (pt) |
BR (1) | BR112015014781A2 (pt) |
CA (1) | CA2893409A1 (pt) |
CL (1) | CL2015001673A1 (pt) |
DK (1) | DK2935601T3 (pt) |
NO (1) | NO2976143T3 (pt) |
WO (1) | WO2014100062A1 (pt) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10513711B2 (en) | 2014-08-13 | 2019-12-24 | Dupont Us Holding, Llc | Genetic targeting in non-conventional yeast using an RNA-guided endonuclease |
EA038321B1 (ru) | 2014-11-06 | 2021-08-09 | Е.И. Дюпон Де Немур Энд Компани | Опосредуемая пептидом доставка направляемой рнк эндонуклеазы в клетки |
EP4144844A1 (en) | 2015-10-12 | 2023-03-08 | DuPont US Holding, LLC | Protected dna templates for gene modification and increased homologous recombination in cells and methods of use |
CN108330114B (zh) * | 2018-03-21 | 2020-11-03 | 北京大学 | 一种利用epa的甘油二酯酰基转移酶及其应用 |
KR102536297B1 (ko) * | 2021-02-08 | 2023-05-24 | 세종대학교산학협력단 | 식물체의 오메가 3 지방산 함량 증가 방법 |
Family Cites Families (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BRPI0407138A (pt) | 2003-02-27 | 2006-01-10 | Basf Plant Science Gmbh | Sequência de ácido nucleico isolada, sequência de aminoácido, construção de gene, vetor, organismo transgênico não humano, processo para produzir ácidos graxos poliinsaturados, óleo, lipìdeo, ou um ácido graxo poliinsaturado ou uma fração dos mesmos, composições de óleo, de lipìdeos, ou de ácido graxo, e, uso do óleo, lipìdeos ou ácidos graxos ou de composições de óleo, de lipìdeos ou de ácido graxo |
ES2421138T3 (es) * | 2003-03-31 | 2013-08-29 | University Of Bristol | Nuevas aciltransferasas vegetales específicas para ácidos grasos poliinsaturados de cadena larga |
US7238482B2 (en) | 2003-05-07 | 2007-07-03 | E. I. Du Pont De Nemours And Company | Production of polyunsaturated fatty acids in oleaginous yeasts |
US20110059496A1 (en) | 2003-06-25 | 2011-03-10 | E. I. Du Pont De Nemours And Company | Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast |
US7267976B2 (en) | 2003-07-02 | 2007-09-11 | E.I. Du Pont De Nemours And Company | Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts |
US7189559B2 (en) * | 2004-11-04 | 2007-03-13 | E. I. Du Pont De Nemours And Company | Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms |
US7588931B2 (en) | 2004-11-04 | 2009-09-15 | E. I. Du Pont De Nemours And Company | High arachidonic acid producing strains of Yarrowia lipolytica |
US20060094102A1 (en) | 2004-11-04 | 2006-05-04 | Zhixiong Xue | Ammonium transporter promoter for gene expression in oleaginous yeast |
DE102004062294A1 (de) * | 2004-12-23 | 2006-07-06 | Basf Plant Science Gmbh | Verfahren zur Herstellung von mehrfach ungesättigten langkettigen Fettsäuren in transgenen Organismen |
US7732155B2 (en) | 2006-12-13 | 2010-06-08 | National Research Council Of Canada | Methods for identifying lysophosphatidylcholine acyltransferases |
CA2701094A1 (en) | 2007-10-03 | 2009-04-09 | E. I. Du Pont De Nemours And Company | Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production |
US8790906B2 (en) * | 2009-03-26 | 2014-07-29 | Suntory Holdings Limited | Lysophospholipid acyltransferase |
AU2010247438B2 (en) * | 2009-05-13 | 2015-01-29 | Basf Plant Science Company Gmbh | Acyltransferases and uses thereof in fatty acid production |
US8524485B2 (en) | 2009-06-16 | 2013-09-03 | E I Du Pont De Nemours And Company | Long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-CoA lysophospholipid acyltransferases |
IN2012DN05162A (pt) * | 2009-12-24 | 2015-10-23 | Du Pont | |
WO2011161093A1 (en) * | 2010-06-25 | 2011-12-29 | Basf Plant Science Company Gmbh | Acyltransferases and uses therof in fatty acid production |
KR20130138760A (ko) * | 2010-08-26 | 2013-12-19 | 이 아이 듀폰 디 네모아 앤드 캄파니 | 고농도의 에이코사펜타엔산 생성을 위한 재조합 미생물 숙주 세포 |
WO2012135773A1 (en) | 2011-03-31 | 2012-10-04 | E. I. Du Pont De Nemours And Company | Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast |
US8906650B2 (en) | 2011-04-01 | 2014-12-09 | E I Du Pont De Nemours And Company | Yarrowia esterase/lipase promoter regions for gene expression in yeast |
EP2694657A1 (en) | 2011-04-05 | 2014-02-12 | E. I. Du Pont de Nemours and Company | Yarrowia n-alkane-hydroxylating cytochrome p450 promoter regions for gene expression in yeast |
US8609369B2 (en) | 2011-04-07 | 2013-12-17 | E I Du Pont De Nemours And Company | Yarrowia peroxisomal 2,4-dienoyl-CoA reductase promoter regions for gene expression in yeast |
MY187101A (en) * | 2012-06-15 | 2021-08-31 | Commw Scient Ind Res Org | Production of long chain polyunsaturated fatty acids in plant cells |
US9347075B2 (en) * | 2012-06-19 | 2016-05-24 | E I Du Pont De Nemours And Company | Nucleic acids encoding mutant acyl-CoA:lysophosphatidylcholine acyltransferases |
BR112015014844A2 (pt) * | 2012-12-21 | 2019-01-15 | Du Pont | célula microbiana recombinante e método de produção de um óleo microbiano |
-
2013
- 2013-12-18 BR BR112015014781A patent/BR112015014781A2/pt not_active Application Discontinuation
- 2013-12-18 DK DK13821271.7T patent/DK2935601T3/en active
- 2013-12-18 CA CA2893409A patent/CA2893409A1/en not_active Abandoned
- 2013-12-18 JP JP2015549594A patent/JP2016502852A/ja active Pending
- 2013-12-18 US US14/132,022 patent/US9701989B2/en active Active
- 2013-12-18 EP EP13821271.7A patent/EP2935601B1/en not_active Not-in-force
- 2013-12-18 WO PCT/US2013/075896 patent/WO2014100062A1/en active Application Filing
-
2014
- 2014-03-24 NO NO14712604A patent/NO2976143T3/no unknown
-
2015
- 2015-06-16 CL CL2015001673A patent/CL2015001673A1/es unknown
Also Published As
Publication number | Publication date |
---|---|
EP2935601B1 (en) | 2018-03-21 |
US9701989B2 (en) | 2017-07-11 |
CL2015001673A1 (es) | 2015-10-23 |
DK2935601T3 (en) | 2018-06-18 |
US20140186906A1 (en) | 2014-07-03 |
WO2014100062A1 (en) | 2014-06-26 |
NO2976143T3 (pt) | 2018-08-04 |
EP2935601A1 (en) | 2015-10-28 |
JP2016502852A (ja) | 2016-02-01 |
CA2893409A1 (en) | 2014-06-26 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
BR112015014781A2 (pt) | célula microbiana recombinante e método de produção de um óleo microbiano | |
Martin et al. | Lipid profile remodeling in response to nitrogen deprivation in the microalgae Chlorella sp.(Trebouxiophyceae) and Nannochloropsis sp.(Eustigmatophyceae) | |
Hishikawa et al. | Diversity and function of membrane glycerophospholipids generated by the remodeling pathway in mammalian cells | |
BRPI1006435A8 (pt) | Molécula de ácido nucleico recombinante e célula hospedeira microbiana | |
Khozin-Goldberg | Lipid metabolism in microalgae | |
BRPI0412052A (pt) | moléculas isoladas de ácido nucléico, polipeptìdeos, gene quimérico, célula hospedeira transformada, métodos para aumentar o teor de triacil glicerol e o teor de ácidos graxos (omega)-3 ou (omega)-6 de triacilgliceróis em uma célula hospedeira transformada | |
Han et al. | Metabolic remodeling of membrane glycerolipids in the microalga Nannochloropsis oceanica under nitrogen deprivation | |
BR112014001907A2 (pt) | produção de dha e outros lc-pufas em plantas | |
BR112013004351A2 (pt) | célula hospedeira microbiana recombinante e método para a produção de um óleo microbiano compreendendo ácido eicosapentaenócio. | |
JP2013128497A5 (pt) | ||
BR112018005976A2 (pt) | óleos de triglicerídeo tendo moléculas de triglicerídeo assimétricas | |
BR112017005370A2 (pt) | acil-acp tioesterases e mutantes das mesmas | |
MX2019013686A (es) | Aceites adaptables. | |
Fan et al. | Biosynthesis of nervonic acid and perspectives for its production by microalgae and other microorganisms | |
MY165098A (en) | Production of dha and other lc-pufas in plants | |
BR112019006856A2 (pt) | aciltransferases, tioesterases variantes e usos das mesmas | |
MX353601B (es) | Aceites adaptables. | |
EP2821492A3 (en) | Acyltransferases and uses thereof in fatty acid production | |
MX2017012800A (es) | Microalgas oleaginosas que tienen una ablación de lpaat. | |
BRPI0909611B8 (pt) | Método de aumento do teor total de ácido graxo de uma célula de oleaginosa, ácido nucleico recombinante, construção de dna recombinante e método de produção de uma semente oleaginosa | |
BR112014001174A2 (pt) | métodos para aumento de rendimentos de produto | |
WO2012087982A3 (en) | Modified photosynthetic microorganisms for producing lipids | |
BR112016029391A2 (pt) | métodos, reagentes e células para biossintetizar compostos | |
BR112014025265A2 (pt) | produção de ácidos graxos poli-insaturados ômega-3 de cadeia longa | |
BR112015005782A2 (pt) | variantes de enzima com propriedades aprimoradas de éster-sintase |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
B11B | Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements |