CL2015001673A1 - Células microbianas recombinantes que producen al menos 28% de ácido aicosapentaenoico como peso seco de la célula - Google Patents

Células microbianas recombinantes que producen al menos 28% de ácido aicosapentaenoico como peso seco de la célula

Info

Publication number
CL2015001673A1
CL2015001673A1 CL2015001673A CL2015001673A CL2015001673A1 CL 2015001673 A1 CL2015001673 A1 CL 2015001673A1 CL 2015001673 A CL2015001673 A CL 2015001673A CL 2015001673 A CL2015001673 A CL 2015001673A CL 2015001673 A1 CL2015001673 A1 CL 2015001673A1
Authority
CL
Chile
Prior art keywords
produce
dry cell
microbial cells
cell weight
recombinant microbial
Prior art date
Application number
CL2015001673A
Other languages
English (en)
Inventor
Quinn Qun Zhu
Seung-Pyo Hong
Pamela L Sharpe
Narendra S Yadav
Xiaochun Fan
Michael Dauner
Original Assignee
E O Du Pont De Nemours And Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by E O Du Pont De Nemours And Company filed Critical E O Du Pont De Nemours And Company
Publication of CL2015001673A1 publication Critical patent/CL2015001673A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6431—Linoleic acids [18:2[n-6]]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01023—1-Acylglycerophosphocholine O-acyltransferase (2.3.1.23), i.e. lysophosphatidylcholine acyltransferase or LPCAT
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
CL2015001673A 2012-12-21 2015-06-16 Células microbianas recombinantes que producen al menos 28% de ácido aicosapentaenoico como peso seco de la célula CL2015001673A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261740502P 2012-12-21 2012-12-21

Publications (1)

Publication Number Publication Date
CL2015001673A1 true CL2015001673A1 (es) 2015-10-23

Family

ID=49956389

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2015001673A CL2015001673A1 (es) 2012-12-21 2015-06-16 Células microbianas recombinantes que producen al menos 28% de ácido aicosapentaenoico como peso seco de la célula

Country Status (9)

Country Link
US (1) US9701989B2 (es)
EP (1) EP2935601B1 (es)
JP (1) JP2016502852A (es)
BR (1) BR112015014781A2 (es)
CA (1) CA2893409A1 (es)
CL (1) CL2015001673A1 (es)
DK (1) DK2935601T3 (es)
NO (1) NO2976143T3 (es)
WO (1) WO2014100062A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10513711B2 (en) 2014-08-13 2019-12-24 Dupont Us Holding, Llc Genetic targeting in non-conventional yeast using an RNA-guided endonuclease
EP3597740B1 (en) 2014-11-06 2022-03-23 DuPont US Holding, LLC Peptide-mediated delivery of rna-guided endonuclease into cells
DK3362560T3 (da) 2015-10-12 2022-11-07 Dupont Us Holding Llc Beskyttede dna-templater til genmodificering og forøget homolog rekombination i celler og fremgangsmåder til anvendelse
CN108330114B (zh) * 2018-03-21 2020-11-03 北京大学 一种利用epa的甘油二酯酰基转移酶及其应用
KR102536297B1 (ko) * 2021-02-08 2023-05-24 세종대학교산학협력단 식물체의 오메가 3 지방산 함량 증가 방법

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0407138A (pt) 2003-02-27 2006-01-10 Basf Plant Science Gmbh Sequência de ácido nucleico isolada, sequência de aminoácido, construção de gene, vetor, organismo transgênico não humano, processo para produzir ácidos graxos poliinsaturados, óleo, lipìdeo, ou um ácido graxo poliinsaturado ou uma fração dos mesmos, composições de óleo, de lipìdeos, ou de ácido graxo, e, uso do óleo, lipìdeos ou ácidos graxos ou de composições de óleo, de lipìdeos ou de ácido graxo
CA2520795C (en) * 2003-03-31 2015-06-23 University Of Bristol Novel plant acyltransferases specific for long-chained, multiply unsaturated fatty acids
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US20110059496A1 (en) 2003-06-25 2011-03-10 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US7267976B2 (en) 2003-07-02 2007-09-11 E.I. Du Pont De Nemours And Company Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts
US7189559B2 (en) * 2004-11-04 2007-03-13 E. I. Du Pont De Nemours And Company Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US20060094102A1 (en) 2004-11-04 2006-05-04 Zhixiong Xue Ammonium transporter promoter for gene expression in oleaginous yeast
US7588931B2 (en) 2004-11-04 2009-09-15 E. I. Du Pont De Nemours And Company High arachidonic acid producing strains of Yarrowia lipolytica
DE102004062294A1 (de) * 2004-12-23 2006-07-06 Basf Plant Science Gmbh Verfahren zur Herstellung von mehrfach ungesättigten langkettigen Fettsäuren in transgenen Organismen
US7732155B2 (en) 2006-12-13 2010-06-08 National Research Council Of Canada Methods for identifying lysophosphatidylcholine acyltransferases
JP5711968B2 (ja) 2007-10-03 2015-05-07 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 高エイコサペンタエン酸を生成するためのヤロウィア・リポリティカ（ＹａｒｒｏｗｉａＬｉｐｏｌｙｔｉｃａ）の最適化株
WO2010110375A1 (ja) * 2009-03-26 2010-09-30 サントリーホールディングス株式会社 新規なリゾリン脂質アシル基転移酵素
CA2760326A1 (en) * 2009-05-13 2010-11-18 Basf Plant Science Company Gmbh Acyltransferases and uses thereof in fatty acid production
US8524485B2 (en) 2009-06-16 2013-09-03 E I Du Pont De Nemours And Company Long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-CoA lysophospholipid acyltransferases
AU2010333827A1 (en) * 2009-12-24 2012-06-28 E. I. Du Pont De Nemours And Company Plant membrane bound O-acyl transferase (MBOAT) family protein sequences and their uses for altering fatty acid compositions
NO2585603T3 (es) * 2010-06-25 2018-05-19
CN103249834B (zh) * 2010-08-26 2016-10-26 纳幕尔杜邦公司 生产高水平二十碳五烯酸的重组微生物宿主细胞
WO2012135773A1 (en) 2011-03-31 2012-10-04 E. I. Du Pont De Nemours And Company Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast
WO2012135777A1 (en) 2011-04-01 2012-10-04 E. I. Du Pont De Nemours And Company Yarrowia esterase/lipase promoter regions for gene expression in yeast
EP2694657A1 (en) 2011-04-05 2014-02-12 E. I. Du Pont de Nemours and Company Yarrowia n-alkane-hydroxylating cytochrome p450 promoter regions for gene expression in yeast
EP2702148A1 (en) 2011-04-07 2014-03-05 E.I. Du Pont De Nemours And Company Yarrowia peroxisomal 2,4-dienoyl-coa reductase promoter regions for gene expression in yeast
PL2861059T3 (pl) * 2012-06-15 2017-10-31 Commw Scient Ind Res Org Wytwarzanie długołańcuchowych wielonienasyconych kwasów tłuszczowych w komórkach roślinnych
US9416382B2 (en) * 2012-06-19 2016-08-16 E I Du Pont De Nemours And Company Production of polyunsaturated fatty acids by coexpression of acyl-CoA:lysophosphatidylcholine acyltransferases and phospholipid:diacylglycerol acyltransferases
US9005940B2 (en) * 2012-12-21 2015-04-14 E I Du Pont De Nemours And Company Down-regulation of a polynucleotide encoding a Sou2 sorbitol utilization protein to modify lipid production in microbial cells

Also Published As

Publication number Publication date
US20140186906A1 (en) 2014-07-03
WO2014100062A1 (en) 2014-06-26
DK2935601T3 (en) 2018-06-18
NO2976143T3 (es) 2018-08-04
BR112015014781A2 (pt) 2017-10-10
US9701989B2 (en) 2017-07-11
JP2016502852A (ja) 2016-02-01
CA2893409A1 (en) 2014-06-26
EP2935601A1 (en) 2015-10-28
EP2935601B1 (en) 2018-03-21

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