BR112014001174A2 - métodos para aumento de rendimentos de produto - Google Patents

métodos para aumento de rendimentos de produto

Info

Publication number
BR112014001174A2
BR112014001174A2 BR112014001174A BR112014001174A BR112014001174A2 BR 112014001174 A2 BR112014001174 A2 BR 112014001174A2 BR 112014001174 A BR112014001174 A BR 112014001174A BR 112014001174 A BR112014001174 A BR 112014001174A BR 112014001174 A2 BR112014001174 A2 BR 112014001174A2
Authority
BR
Brazil
Prior art keywords
acetyl
coa
naturally occurring
sufficient
occurring microbial
Prior art date
Application number
BR112014001174A
Other languages
English (en)
Inventor
P Burgard Anthony
Sun Jun
Pharkya Priti
E Osterhout Robin
Original Assignee
Genomatica Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genomatica Inc filed Critical Genomatica Inc
Publication of BR112014001174A2 publication Critical patent/BR112014001174A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

abstract of the disclosure a non-naturally occurring microbial organism includes a microbial organism having a reductive tca or wood-ljungdahl pathway in which at least one exogenous nucleic acid encoding these pathway enzymes is expressed in a sufficient amount to enhance carbon flux through acetyl-coa. a method for enhancing carbon flux through acetyl-coa includes culturing theses non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce a product having acetyl-coa as a building block. another non-naturally occurring microbial organism includes at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock. a method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen includes culturing this organism for a sufficient period of time to produce a product. tradução do resumo resumo patente de invenção: "métodos para aumento de rendimentos de produto". um organismo microbiano que não ocorre naturalmente inclui um organismo microbiano tendo uma via de tca redutiva ou via de wood-ljungdahl na qual pelo menos um ácido nucleico exógeno que codifica enzimas destas vias é expresso em uma quantidade suficiente para aumentar o fluxo de carbono através de acetil-coa. um método para intensificação de fluxo de carbono através de acetil-coa inclui cultura desses organismos microbianos que não ocorrem naturalmente sob condições e durante um período de tempo suficiente para produzir um produto tendo acetil-coa como um bloco de construção. outro organismo microbiano que não ocorre naturalmente inclui pelo menos um ácido nucleico exógeno que codifica uma enzima expressa em uma quantidade suficiente para aumentar a disponibilidade de equivalentes de redução na presença de monóxido de carbono ou hidrogênio, desse modo, aumentando o rendimento de produtos redox-limitados via uma matéria-prima de carbono com base em carboidrato. um método para intensificação da disponibilidade de equivalentes de redução na presença de monóxido de carbono ou hidrogênio inclui cultura desse organismo durante um período de tempo suficiente para produzir um produto.
BR112014001174A 2011-07-20 2012-07-19 métodos para aumento de rendimentos de produto BR112014001174A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161510066P 2011-07-20 2011-07-20
US201161512376P 2011-07-27 2011-07-27
PCT/US2012/047278 WO2013012975A1 (en) 2011-07-20 2012-07-19 Methods for increasing product yields

Publications (1)

Publication Number Publication Date
BR112014001174A2 true BR112014001174A2 (pt) 2017-02-21

Family

ID=47558462

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014001174A BR112014001174A2 (pt) 2011-07-20 2012-07-19 métodos para aumento de rendimentos de produto

Country Status (3)

Country Link
EP (1) EP2734627A4 (pt)
BR (1) BR112014001174A2 (pt)
WO (1) WO2013012975A1 (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102097065B1 (ko) * 2013-08-23 2020-04-03 삼성전자주식회사 4-히드록시부티레이트 생산 균주 및 이를 이용한 4-히드록시부티레이트의 혐기적 생산 방법
SI3077501T1 (sl) 2013-12-03 2022-02-28 Genomatica, Inc. Mikroorganizmi in metode za izboljšanje donosa izdelkov z uporabo sinteze acetil-coa
EP3744830B1 (en) 2013-12-27 2023-11-22 Genomatica, Inc. Methods and organisms with increased carbon flux efficiencies
US20170298363A1 (en) 2014-09-18 2017-10-19 Genomatica, Inc Non-natural microbial organisms with improved energetic efficiency
CA2977593A1 (en) 2015-02-27 2016-09-01 White Dog Labs, Inc. Mixotrophic fermentation method for making acetone, isopropanol, butyric acid and other bioproducts, and mixtures thereof
CN108026214B (zh) 2015-05-30 2021-03-02 基因组股份公司 乙烯基异构酶-脱水酶、烯醇脱水酶、芳樟醇脱水酶和/或巴豆醇脱水酶及其制备和使用
US20180148743A1 (en) * 2015-07-29 2018-05-31 Evonik Degussa Gmbh Production of 3-hydroxybutyrate
CN115322261A (zh) 2015-10-30 2022-11-11 基因组股份公司 甲醇脱氢酶融合蛋白
WO2018150377A2 (en) * 2017-02-17 2018-08-23 Industrial Microbes, Inc. Culture modified to convert methane or methanol to 3-hydroxyproprionate
US20210079334A1 (en) 2018-01-30 2021-03-18 Genomatica, Inc. Fermentation systems and methods with substantially uniform volumetric uptake rate of a reactive gaseous component
WO2020006058A2 (en) 2018-06-26 2020-01-02 Genomatica, Inc. Engineered microorganisms with g3p---> 3pg enzyme and/or fructose-1,6-bisphosphatase including those having synthetic or enhanced methylotrophy

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009062190A2 (en) * 2007-11-10 2009-05-14 Joule Biotechnologies, Inc. Hyperphotosynthetic organisms
EP2285948B1 (en) * 2008-03-03 2014-01-08 Joule Unlimited Technologies, Inc. Engineered co2 fixing microorganisms producing carbon-based products of interest
JP5964747B2 (ja) * 2009-06-04 2016-08-03 ゲノマチカ， インク． １，４−ブタンジオールの生成のための微生物体及び関連する方法
WO2011031897A1 (en) * 2009-09-09 2011-03-17 Genomatica, Inc. Microorganisms and methods for the co-production of isopropanol with primary alcohols, diols and acids

Also Published As

Publication number Publication date
EP2734627A4 (en) 2015-07-22
EP2734627A1 (en) 2014-05-28
WO2013012975A1 (en) 2013-01-24

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Legal Events

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B08K Lapse as no evidence of payment of the annual fee has been furnished to inpi (acc. art. 87)