CN102803289B - 用于高水平生产二十碳五烯酸的改善的优化的解脂耶氏酵母菌株 - Google Patents

用于高水平生产二十碳五烯酸的改善的优化的解脂耶氏酵母菌株 Download PDF

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Publication number
CN102803289B
CN102803289B CN201080026332.7A CN201080026332A CN102803289B CN 102803289 B CN102803289 B CN 102803289B CN 201080026332 A CN201080026332 A CN 201080026332A CN 102803289 B CN102803289 B CN 102803289B
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epa
gene
strain
strains
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CN201080026332.7A
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Chinese (zh)
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CN102803289A (zh
Inventor
S-P·洪
P·L·夏普
Z·薛
N·S·亚达夫
Q·朱
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EIDP Inc
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EI Du Pont de Nemours and Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201080026332.7A 2009-06-16 2010-06-14 用于高水平生产二十碳五烯酸的改善的优化的解脂耶氏酵母菌株 Expired - Fee Related CN102803289B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18736609P 2009-06-16 2009-06-16
US61/187366 2009-06-16
PCT/US2010/038535 WO2010147904A1 (en) 2009-06-16 2010-06-14 Improved optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production

Publications (2)

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CN102803289A CN102803289A (zh) 2012-11-28
CN102803289B true CN102803289B (zh) 2015-07-22

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CN201080026332.7A Expired - Fee Related CN102803289B (zh) 2009-06-16 2010-06-14 用于高水平生产二十碳五烯酸的改善的优化的解脂耶氏酵母菌株

Country Status (9)

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EP (1) EP2475679B1 (enExample)
JP (1) JP5893555B2 (enExample)
CN (1) CN102803289B (enExample)
AU (1) AU2010260231B2 (enExample)
BR (1) BRPI1009649A2 (enExample)
CA (1) CA2761589C (enExample)
CL (1) CL2011003162A1 (enExample)
DK (1) DK2475679T3 (enExample)
WO (1) WO2010147904A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120040076A1 (en) * 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company Aquaculture feed compositions
WO2013192007A1 (en) * 2012-06-19 2013-12-27 E. I. Du Pont De Nemours And Company MUTANT ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES
CN108330114B (zh) * 2018-03-21 2020-11-03 北京大学 一种利用epa的甘油二酯酰基转移酶及其应用
WO2020082221A1 (zh) * 2018-10-23 2020-04-30 利时雨 重组磷酸胆碱胞苷转移酶的粘红酵母携带外源多肽的活细胞脂质体及其应用
CN117568177A (zh) * 2023-11-16 2024-02-20 鲁东大学 一种解脂耶氏酵母提取物的制备方法及应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101018862A (zh) * 2004-04-22 2007-08-15 联邦科学技术研究组织 用重组细胞合成长链多不饱和脂肪酸
CN101111601A (zh) * 2004-11-04 2008-01-23 纳幕尔杜邦公司 解脂耶氏酵母的高二十碳五烯酸生产菌株
WO2009046248A1 (en) * 2007-10-03 2009-04-09 E. I. Du Pont De Nemours And Company Peroxisome biogenesis factor protein (pex) disruptions for altering the content of polyunsaturated fatty acids and the total lipid content in oleaginous eukaryotic organisms

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4670285A (en) 1982-08-06 1987-06-02 The University Of Toronto Innovations Foundation Infant formula
US5648564A (en) 1995-12-21 1997-07-15 Kemin Industries, Inc. Process for the formation, isolation and purification of comestible xanthophyll crystals from plants
US6797303B2 (en) 2001-09-04 2004-09-28 Lycored Natural Products Industries Ltd. Carotenoid extraction process
ATE517984T1 (de) * 2003-02-27 2011-08-15 Basf Plant Science Gmbh Verfahren zur herstellung mehrfach ungesättigter fettsäuren
EP3121269A1 (de) 2003-03-31 2017-01-25 University Of Bristol Neue pflanzliche acyltransferase spezifisch für langkettige mehrfach ungesättigte fettsäuren
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7259255B2 (en) 2003-06-25 2007-08-21 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US7504259B2 (en) 2003-11-12 2009-03-17 E. I. Du Pont De Nemours And Company Δ12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast
US7550651B2 (en) 2004-06-25 2009-06-23 E.I. Du Pont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
DE102004062294A1 (de) 2004-12-23 2006-07-06 Basf Plant Science Gmbh Verfahren zur Herstellung von mehrfach ungesättigten langkettigen Fettsäuren in transgenen Organismen
US7772444B2 (en) * 2005-05-19 2010-08-10 E. I. Du Pont De Nemours And Company Method for the production of resveratrol in a recombinant oleaginous microorganism
US7470532B2 (en) 2005-10-19 2008-12-30 E.I. Du Pont De Nemours And Company Mortierella alpina C16/18 fatty acid elongase
WO2007061742A1 (en) 2005-11-23 2007-05-31 E. I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
WO2008054565A1 (en) 2006-10-30 2008-05-08 E. I. Du Pont De Nemours And Company Δ17 desaturase and its use in making polyunsaturated fatty acids
US7709239B2 (en) 2006-12-07 2010-05-04 E.I. Du Pont De Nemours And Company Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
US8846374B2 (en) 2006-12-12 2014-09-30 E I Du Pont De Nemours And Company Carotenoid production in a recombinant oleaginous yeast
DK2129777T3 (en) * 2007-04-03 2016-01-25 Du Pont MULTIZYMER AND USE THEREOF FOR THE PRODUCTION OF polyunsaturated fatty acids
WO2009001315A2 (en) 2007-06-26 2008-12-31 Staahl Ulf Use of a class of genes encoding lysophospholipid acyl transferases for application in agriculture, biotechnology, and medicine
RU2496881C2 (ru) 2007-07-23 2013-10-27 Сантори Холдингз Лимитед Композиции жирных кислот, имеющие новую пропорцию жирных кислот
US8323935B2 (en) 2007-10-03 2012-12-04 E I Du Pont De Nemours And Company Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production
CA2744716C (en) 2008-12-18 2017-05-23 E.I. Du Pont De Nemours And Company Reducing byproduction of malonates in a fermentation process

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101018862A (zh) * 2004-04-22 2007-08-15 联邦科学技术研究组织 用重组细胞合成长链多不饱和脂肪酸
CN101111601A (zh) * 2004-11-04 2008-01-23 纳幕尔杜邦公司 解脂耶氏酵母的高二十碳五烯酸生产菌株
WO2009046248A1 (en) * 2007-10-03 2009-04-09 E. I. Du Pont De Nemours And Company Peroxisome biogenesis factor protein (pex) disruptions for altering the content of polyunsaturated fatty acids and the total lipid content in oleaginous eukaryotic organisms

Also Published As

Publication number Publication date
WO2010147904A1 (en) 2010-12-23
DK2475679T3 (da) 2016-05-09
CL2011003162A1 (es) 2012-07-20
AU2010260231A1 (en) 2011-11-10
EP2475679A4 (en) 2013-04-03
CN102803289A (zh) 2012-11-28
BRPI1009649A2 (pt) 2016-03-15
CA2761589C (en) 2017-12-19
CA2761589A1 (en) 2010-12-23
JP5893555B2 (ja) 2016-03-23
JP2012529913A (ja) 2012-11-29
AU2010260231B2 (en) 2017-03-02
EP2475679B1 (en) 2016-02-10
AU2010260231A8 (en) 2011-11-17
EP2475679A1 (en) 2012-07-18

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