DK2475679T3 - Forbedrede, optimerede stammer af yarrowia lipolytica til fremstilling af højkoncentreret eicosapentaensyre - Google Patents
Forbedrede, optimerede stammer af yarrowia lipolytica til fremstilling af højkoncentreret eicosapentaensyre Download PDFInfo
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- DK2475679T3 DK2475679T3 DK10790007.8T DK10790007T DK2475679T3 DK 2475679 T3 DK2475679 T3 DK 2475679T3 DK 10790007 T DK10790007 T DK 10790007T DK 2475679 T3 DK2475679 T3 DK 2475679T3
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- 235000021283 resveratrol Nutrition 0.000 description 1
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- 238000012216 screening Methods 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
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- 238000004088 simulation Methods 0.000 description 1
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- PRWXGRGLHYDWPS-UHFFFAOYSA-L sodium malonate Chemical compound [Na+].[Na+].[O-]C(=O)CC([O-])=O PRWXGRGLHYDWPS-UHFFFAOYSA-L 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
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- JIWBIWFOSCKQMA-UHFFFAOYSA-N stearidonic acid Natural products CCC=CCC=CCC=CCC=CCCCCC(O)=O JIWBIWFOSCKQMA-UHFFFAOYSA-N 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 235000021092 sugar substitutes Nutrition 0.000 description 1
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- 239000000516 sunscreening agent Substances 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
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- 235000020357 syrup Nutrition 0.000 description 1
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- 230000008685 targeting Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- 229960003495 thiamine Drugs 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
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- 150000003595 thromboxanes Chemical class 0.000 description 1
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- DJJCXFVJDGTHFX-XVFCMESISA-N uridine 5'-monophosphate Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]1N1C(=O)NC(=O)C=C1 DJJCXFVJDGTHFX-XVFCMESISA-N 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- IBIDRSSEHFLGSD-UHFFFAOYSA-N valinyl-arginine Natural products CC(C)C(N)C(=O)NC(C(O)=O)CCCN=C(N)N IBIDRSSEHFLGSD-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US18736609P | 2009-06-16 | 2009-06-16 | |
| PCT/US2010/038535 WO2010147904A1 (en) | 2009-06-16 | 2010-06-14 | Improved optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2475679T3 true DK2475679T3 (da) | 2016-05-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| DK10790007.8T DK2475679T3 (da) | 2009-06-16 | 2010-06-14 | Forbedrede, optimerede stammer af yarrowia lipolytica til fremstilling af højkoncentreret eicosapentaensyre |
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|---|---|
| EP (1) | EP2475679B1 (enExample) |
| JP (1) | JP5893555B2 (enExample) |
| CN (1) | CN102803289B (enExample) |
| AU (1) | AU2010260231B2 (enExample) |
| BR (1) | BRPI1009649A2 (enExample) |
| CA (1) | CA2761589C (enExample) |
| CL (1) | CL2011003162A1 (enExample) |
| DK (1) | DK2475679T3 (enExample) |
| WO (1) | WO2010147904A1 (enExample) |
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| US20120040076A1 (en) * | 2010-08-11 | 2012-02-16 | E. I. Du Pont De Nemours And Company | Aquaculture feed compositions |
| WO2013192007A1 (en) * | 2012-06-19 | 2013-12-27 | E. I. Du Pont De Nemours And Company | MUTANT ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES |
| CN108330114B (zh) * | 2018-03-21 | 2020-11-03 | 北京大学 | 一种利用epa的甘油二酯酰基转移酶及其应用 |
| WO2020082221A1 (zh) * | 2018-10-23 | 2020-04-30 | 利时雨 | 重组磷酸胆碱胞苷转移酶的粘红酵母携带外源多肽的活细胞脂质体及其应用 |
| CN117568177A (zh) * | 2023-11-16 | 2024-02-20 | 鲁东大学 | 一种解脂耶氏酵母提取物的制备方法及应用 |
Family Cites Families (24)
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| US4670285A (en) | 1982-08-06 | 1987-06-02 | The University Of Toronto Innovations Foundation | Infant formula |
| US5648564A (en) | 1995-12-21 | 1997-07-15 | Kemin Industries, Inc. | Process for the formation, isolation and purification of comestible xanthophyll crystals from plants |
| US6797303B2 (en) | 2001-09-04 | 2004-09-28 | Lycored Natural Products Industries Ltd. | Carotenoid extraction process |
| ATE517984T1 (de) * | 2003-02-27 | 2011-08-15 | Basf Plant Science Gmbh | Verfahren zur herstellung mehrfach ungesättigter fettsäuren |
| EP3121269A1 (de) | 2003-03-31 | 2017-01-25 | University Of Bristol | Neue pflanzliche acyltransferase spezifisch für langkettige mehrfach ungesättigte fettsäuren |
| US7238482B2 (en) | 2003-05-07 | 2007-07-03 | E. I. Du Pont De Nemours And Company | Production of polyunsaturated fatty acids in oleaginous yeasts |
| US7259255B2 (en) | 2003-06-25 | 2007-08-21 | E. I. Du Pont De Nemours And Company | Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast |
| US7504259B2 (en) | 2003-11-12 | 2009-03-17 | E. I. Du Pont De Nemours And Company | Δ12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast |
| CN101018862B (zh) * | 2004-04-22 | 2013-10-09 | 联邦科学技术研究组织 | 用重组细胞合成长链多不饱和脂肪酸 |
| US7550651B2 (en) | 2004-06-25 | 2009-06-23 | E.I. Du Pont De Nemours And Company | Delta-8 desaturase and its use in making polyunsaturated fatty acids |
| US7550286B2 (en) * | 2004-11-04 | 2009-06-23 | E. I. Du Pont De Nemours And Company | Docosahexaenoic acid producing strains of Yarrowia lipolytica |
| DE102004062294A1 (de) | 2004-12-23 | 2006-07-06 | Basf Plant Science Gmbh | Verfahren zur Herstellung von mehrfach ungesättigten langkettigen Fettsäuren in transgenen Organismen |
| US7772444B2 (en) * | 2005-05-19 | 2010-08-10 | E. I. Du Pont De Nemours And Company | Method for the production of resveratrol in a recombinant oleaginous microorganism |
| US7470532B2 (en) | 2005-10-19 | 2008-12-30 | E.I. Du Pont De Nemours And Company | Mortierella alpina C16/18 fatty acid elongase |
| WO2007061742A1 (en) | 2005-11-23 | 2007-05-31 | E. I. Du Pont De Nemours And Company | Delta-9 elongases and their use in making polyunsaturated fatty acids |
| WO2008054565A1 (en) | 2006-10-30 | 2008-05-08 | E. I. Du Pont De Nemours And Company | Δ17 desaturase and its use in making polyunsaturated fatty acids |
| US7709239B2 (en) | 2006-12-07 | 2010-05-04 | E.I. Du Pont De Nemours And Company | Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids |
| US8846374B2 (en) | 2006-12-12 | 2014-09-30 | E I Du Pont De Nemours And Company | Carotenoid production in a recombinant oleaginous yeast |
| DK2129777T3 (en) * | 2007-04-03 | 2016-01-25 | Du Pont | MULTIZYMER AND USE THEREOF FOR THE PRODUCTION OF polyunsaturated fatty acids |
| WO2009001315A2 (en) | 2007-06-26 | 2008-12-31 | Staahl Ulf | Use of a class of genes encoding lysophospholipid acyl transferases for application in agriculture, biotechnology, and medicine |
| RU2496881C2 (ru) | 2007-07-23 | 2013-10-27 | Сантори Холдингз Лимитед | Композиции жирных кислот, имеющие новую пропорцию жирных кислот |
| CA2701130A1 (en) | 2007-10-03 | 2009-04-09 | E.I. Du Pont De Nemours And Company | Peroxisome biogenesis factor protein (pex) disruptions for altering the content of polyunsaturated fatty acids and the total lipid content in oleaginous eukaryotic organisms |
| US8323935B2 (en) | 2007-10-03 | 2012-12-04 | E I Du Pont De Nemours And Company | Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production |
| CA2744716C (en) | 2008-12-18 | 2017-05-23 | E.I. Du Pont De Nemours And Company | Reducing byproduction of malonates in a fermentation process |
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2010
- 2010-06-14 CN CN201080026332.7A patent/CN102803289B/zh not_active Expired - Fee Related
- 2010-06-14 JP JP2012516173A patent/JP5893555B2/ja not_active Expired - Fee Related
- 2010-06-14 DK DK10790007.8T patent/DK2475679T3/da active
- 2010-06-14 EP EP10790007.8A patent/EP2475679B1/en not_active Not-in-force
- 2010-06-14 CA CA2761589A patent/CA2761589C/en not_active Expired - Fee Related
- 2010-06-14 WO PCT/US2010/038535 patent/WO2010147904A1/en not_active Ceased
- 2010-06-14 AU AU2010260231A patent/AU2010260231B2/en not_active Ceased
- 2010-06-14 BR BRPI1009649A patent/BRPI1009649A2/pt not_active IP Right Cessation
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2011
- 2011-12-15 CL CL2011003162A patent/CL2011003162A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2010147904A1 (en) | 2010-12-23 |
| CN102803289B (zh) | 2015-07-22 |
| CL2011003162A1 (es) | 2012-07-20 |
| AU2010260231A1 (en) | 2011-11-10 |
| EP2475679A4 (en) | 2013-04-03 |
| CN102803289A (zh) | 2012-11-28 |
| BRPI1009649A2 (pt) | 2016-03-15 |
| CA2761589C (en) | 2017-12-19 |
| CA2761589A1 (en) | 2010-12-23 |
| JP5893555B2 (ja) | 2016-03-23 |
| JP2012529913A (ja) | 2012-11-29 |
| AU2010260231B2 (en) | 2017-03-02 |
| EP2475679B1 (en) | 2016-02-10 |
| AU2010260231A8 (en) | 2011-11-17 |
| EP2475679A1 (en) | 2012-07-18 |
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