CA3225136A1 - Methods of culturing a mammalian cell - Google Patents

Methods of culturing a mammalian cell Download PDF

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Publication number
CA3225136A1
CA3225136A1 CA3225136A CA3225136A CA3225136A1 CA 3225136 A1 CA3225136 A1 CA 3225136A1 CA 3225136 A CA3225136 A CA 3225136A CA 3225136 A CA3225136 A CA 3225136A CA 3225136 A1 CA3225136 A1 CA 3225136A1
Authority
CA
Canada
Prior art keywords
concentration
days
liquid medium
poloxamer
greater concentration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3225136A
Other languages
English (en)
French (fr)
Inventor
Christopher HWANG
Timothy Johnson
Jason Walther
Cheng Cheng
Jonathan Wang
Neha Shah
Seul-A Bae
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genzyme Corp
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Corp filed Critical Genzyme Corp
Publication of CA3225136A1 publication Critical patent/CA3225136A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0043—Medium free of human- or animal-derived components
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/005—Protein-free medium
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/50—Soluble polymers, e.g. polyethyleneglycol [PEG]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sustainable Development (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
CA3225136A 2014-12-22 2015-12-21 Methods of culturing a mammalian cell Pending CA3225136A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462095734P 2014-12-22 2014-12-22
US62/095,734 2014-12-22
CA2971861A CA2971861C (en) 2014-12-22 2015-12-21 Methods of culturing a mammalian cell

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2971861A Division CA2971861C (en) 2014-12-22 2015-12-21 Methods of culturing a mammalian cell

Publications (1)

Publication Number Publication Date
CA3225136A1 true CA3225136A1 (en) 2016-06-30

Family

ID=55527619

Family Applications (2)

Application Number Title Priority Date Filing Date
CA3225136A Pending CA3225136A1 (en) 2014-12-22 2015-12-21 Methods of culturing a mammalian cell
CA2971861A Active CA2971861C (en) 2014-12-22 2015-12-21 Methods of culturing a mammalian cell

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2971861A Active CA2971861C (en) 2014-12-22 2015-12-21 Methods of culturing a mammalian cell

Country Status (15)

Country Link
US (2) US11306341B2 (enExample)
EP (1) EP3237604B1 (enExample)
JP (4) JP6843750B2 (enExample)
KR (3) KR102769649B1 (enExample)
CN (2) CN107567495A (enExample)
AU (3) AU2015369809B2 (enExample)
BR (1) BR112017013423B1 (enExample)
CA (2) CA3225136A1 (enExample)
ES (1) ES3066905T3 (enExample)
IL (2) IL253071B2 (enExample)
MX (2) MX383857B (enExample)
RU (1) RU2712746C2 (enExample)
SG (2) SG11201705103WA (enExample)
TW (3) TWI741851B (enExample)
WO (1) WO2016106192A2 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105209603A (zh) 2013-02-22 2015-12-30 建新公司 微载体灌注培养方法及其用途
CN105143443A (zh) 2013-02-22 2015-12-09 建新公司 微载体灌注培养方法及其用途
TWI743024B (zh) 2014-06-06 2021-10-21 美商健臻公司 灌注培養方法及其用途
TWI776235B (zh) 2014-06-09 2022-09-01 美商健臻公司 種子罐培養法(seed train processes)及其用途
KR102769649B1 (ko) 2014-12-22 2025-02-19 젠자임 코포레이션 포유류 세포의 배양 방법
MA52007A (fr) 2018-03-16 2021-01-20 Genzyme Corp Procédés d'amélioration de la viabilité cellulaire dans un bioréacteur de production
WO2022035973A1 (en) * 2020-08-12 2022-02-17 Allen Institute Systems, devices, and methods for quality control of cell production using predictive tagging
CN112795531B (zh) * 2021-04-11 2021-08-06 依科赛生物科技（太仓）有限公司 一种cho细胞无血清无蛋白培养基及其用途
IL307715A (en) * 2021-04-14 2023-12-01 Genzyme Corp Perfusion methods for growing a mammalian cell culture
WO2023054556A1 (ja) * 2021-09-30 2023-04-06 富士フイルム株式会社 細胞培養方法、及び有用物質の製造方法
CN119968454A (zh) 2022-09-28 2025-05-09 建新公司 用于生产生物产品的系统和装置及其使用方法

Family Cites Families (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4522811A (en) 1982-07-08 1985-06-11 Syntex (U.S.A.) Inc. Serial injection of muramyldipeptides and liposomes enhances the anti-infective activity of muramyldipeptides
US5658782A (en) 1993-10-20 1997-08-19 State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University A Non-Profit Organization Amino acid transporters and uses
US5856179A (en) 1994-03-10 1999-01-05 Genentech, Inc. Polypeptide production in animal cell culture
US5721121A (en) 1995-06-06 1998-02-24 Genentech, Inc. Mammalian cell culture process for producing a tumor necrosis factor receptor immunoglobulin chimeric protein
US6458574B1 (en) * 1996-09-12 2002-10-01 Transkaryotic Therapies, Inc. Treatment of a α-galactosidase a deficiency
DE60124780T2 (de) 2000-12-20 2007-10-11 Bayer Pharmaceuticals Corp., West Haven Vorrichtung und verfahren zur serienvermehrung von säugerzellen zum animpfen
US20030087372A1 (en) 2001-06-13 2003-05-08 Genentech, Inc. Methods of culturing animal cells and polypeptide production in animal cells
WO2003039459A2 (en) 2001-11-05 2003-05-15 Genvec, Inc. Viral vector production methods and compositions
US6953692B2 (en) 2001-12-18 2005-10-11 Bayer Pharmaceuticals Corporation Device and method for seed-train expansion of mammalian cells
US7067279B1 (en) * 2002-08-23 2006-06-27 Immunex Corporation Cell culture performance with betaine
US6974681B1 (en) 2002-08-23 2005-12-13 Immunex Corporation Cell culture performance with vanadate
FR2866655B1 (fr) 2004-02-20 2007-05-11 Cesco Bioengineering Co Ltd Appareil et procede pour la preparation et la mise en culture de cellules
PL1720972T3 (pl) 2004-03-05 2014-06-30 Dpx Holdings Bv Proces hodowli komórek poprzez ciągłą perfuzję i zmienny przepływ styczny
AU2005287162B2 (en) 2004-09-16 2010-12-23 Becton, Dickinson And Company Perfusion bioreactor for culturing cells
DK1891205T3 (en) 2005-06-15 2018-03-19 Zhejiang Jinyishengshi Bioengineering Co Ltd SUSPENSION CULTURE CONTAINERS
WO2006138143A1 (en) 2005-06-15 2006-12-28 Amprotein Corporation Suspension culture vessels
US20080206819A1 (en) * 2006-08-21 2008-08-28 Mary Tsao Intensified Perfusion Production Method
TW201516149A (zh) 2006-09-13 2015-05-01 Abbvie Inc 細胞培養改良
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
WO2008106515A1 (en) 2007-02-27 2008-09-04 The Board Of Trustees Of The University Of Illinois Insert device for multiwell plate
WO2009034186A2 (en) 2007-09-13 2009-03-19 Helmholtz-Zentrum für Infektionsforschung GmbH Process for cell cultivation
AU2008325141B2 (en) * 2007-11-08 2014-03-20 Ambit Biosciences Corp. Methods of administering N-(5-tert-butyl-isoxazol-3-yl)-N'-{4-[7-(2-morpholin-4-yl-ethoxy)imidazo[2,1-b][1,3]benzothiazol-2-yl]phenyl}urea to treat proliferative disease
SG192471A1 (en) * 2007-12-31 2013-08-30 Baxter Int Substantially animal protein-free recombinant furin and methods for producing same
US20090233334A1 (en) * 2008-03-11 2009-09-17 Excellgene Sa Cell cultivation and production of recombinant proteins by means of an orbital shake bioreactor system with disposable bags at the 1,500 liter scale
DE102008019691A1 (de) 2008-04-15 2009-10-29 Technische Universität Ilmenau Teilaktives mikrofluidisches System für die 3D-Zellkultivierung sowie Verfahren zu dessen Perfusion
WO2010120514A2 (en) 2009-03-31 2010-10-21 The Trustees Of The University Of Pennsylvania Antigen-binding proteins comprising recombinant protein scaffolds
KR101131867B1 (ko) 2009-08-13 2012-04-03 (주)히스토스템 폴리감마글루탐산을 포함하는 배양 세포 손상 방지를 위한 배지 첨가용 조성물
DK2501822T3 (da) * 2009-11-17 2017-11-27 Squibb & Sons Llc Fremgangsmåder til forbedret proteinproduktion
AU2012252305B2 (en) 2011-05-12 2017-02-02 Probiogen Ag Enhancement of protein production yield mediated by a fast shuttling cdc42 gtpase
WO2013116449A1 (en) 2012-02-02 2013-08-08 Corning Incorporated Automatic continuous perfusion cell culture microplate consumables
SG10201803961VA (en) 2012-04-01 2018-07-30 Emd Millipore Corp Cell culture and gradient migration assay methods and devices
SG10201901708SA (en) 2012-10-23 2019-03-28 Genzyme Corp Perfusion culturing methods and uses thereof
CN105209603A (zh) 2013-02-22 2015-12-30 建新公司 微载体灌注培养方法及其用途
CN105143443A (zh) 2013-02-22 2015-12-09 建新公司 微载体灌注培养方法及其用途
AU2014226126B2 (en) 2013-03-08 2019-02-14 Genzyme Corporation Continuous purification of therapeutic proteins
US20140271633A1 (en) 2013-03-14 2014-09-18 Abbvie Inc. Mammalian cell culture performance through surfactant supplementation of feed media
TWI656839B (zh) 2013-03-15 2019-04-21 健臻公司 高密度細胞儲存方法
WO2014194137A1 (en) * 2013-05-29 2014-12-04 Biogen Idec Ma Inc. Methods of evaluating cell culture additives
ES2891728T3 (es) 2013-09-16 2022-01-31 Genzyme Corp Métodos y sistemas para procesar un cultivo celular
TWI709570B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析法及製法
TWI709569B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析樹脂及其用於製造方法的用途
KR102391259B1 (ko) 2014-06-04 2022-04-26 암젠 인크 포유류 세포 배양물을 회수하는 방법
TWI743024B (zh) 2014-06-06 2021-10-21 美商健臻公司 灌注培養方法及其用途
TWI776235B (zh) 2014-06-09 2022-09-01 美商健臻公司 種子罐培養法(seed train processes)及其用途
KR102769649B1 (ko) 2014-12-22 2025-02-19 젠자임 코포레이션 포유류 세포의 배양 방법
WO2018178069A1 (en) 2017-03-31 2018-10-04 Boehringer Ingelheim International Gmbh Perfusion medium
WO2021058713A1 (en) 2019-09-27 2021-04-01 Boehringer Ingelheim International Gmbh Concentrated perfusion medium
IL307715A (en) 2021-04-14 2023-12-01 Genzyme Corp Perfusion methods for growing a mammalian cell culture

Also Published As

Publication number Publication date
IL253071A0 (en) 2017-08-31
US20220145348A1 (en) 2022-05-12
US11306341B2 (en) 2022-04-19
KR102568951B1 (ko) 2023-08-22
KR102769649B1 (ko) 2025-02-19
TW202106706A (zh) 2021-02-16
AU2022204152B2 (en) 2024-11-28
MX2021007433A (es) 2021-08-05
JP2022107000A (ja) 2022-07-20
ES3066905T3 (en) 2026-05-18
HK1246345A1 (en) 2018-09-07
TW201639881A (zh) 2016-11-16
CA2971861C (en) 2024-02-13
EP3237604A2 (en) 2017-11-01
TWI741851B (zh) 2021-10-01
JP7075463B2 (ja) 2022-05-25
AU2015369809B2 (en) 2022-03-17
WO2016106192A3 (en) 2016-11-03
KR20170090508A (ko) 2017-08-07
TWI843012B (zh) 2024-05-21
RU2020101112A (ru) 2020-03-13
RU2017126045A (ru) 2019-01-24
IL253071B2 (en) 2024-04-01
TWI751103B (zh) 2022-01-01
MX383857B (es) 2025-03-14
SG10202001034PA (en) 2020-04-29
JP2017538435A (ja) 2017-12-28
TW202200785A (zh) 2022-01-01
AU2025201416A1 (en) 2025-03-20
JP6843750B2 (ja) 2021-03-17
CA2971861A1 (en) 2016-06-30
EP3237604C0 (en) 2026-02-11
SG11201705103WA (en) 2017-07-28
AU2022204152A1 (en) 2022-06-30
BR112017013423A2 (pt) 2018-01-09
RU2017126045A3 (enExample) 2019-06-24
US12486524B2 (en) 2025-12-02
IL308064A (en) 2023-12-01
AU2015369809A1 (en) 2017-08-10
CN107567495A (zh) 2018-01-09
EP3237604B1 (en) 2026-02-11
IL253071B1 (en) 2023-12-01
US20160177361A1 (en) 2016-06-23
JP2024050754A (ja) 2024-04-10
CN122326537A (zh) 2026-07-03
MX2017008338A (es) 2017-10-19
RU2712746C2 (ru) 2020-01-30
JP2021019624A (ja) 2021-02-18
KR20230125102A (ko) 2023-08-28
KR20250025516A (ko) 2025-02-21
WO2016106192A2 (en) 2016-06-30
BR112017013423B1 (pt) 2023-04-11

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