SG10201803961VA - Cell culture and gradient migration assay methods and devices - Google Patents

Cell culture and gradient migration assay methods and devices

Info

Publication number
SG10201803961VA
SG10201803961VA SG10201803961VA SG10201803961VA SG10201803961VA SG 10201803961V A SG10201803961V A SG 10201803961VA SG 10201803961V A SG10201803961V A SG 10201803961VA SG 10201803961V A SG10201803961V A SG 10201803961VA SG 10201803961V A SG10201803961V A SG 10201803961VA
Authority
SG
Singapore
Prior art keywords
devices
cell culture
migration assay
assay methods
gradient migration
Prior art date
Application number
SG10201803961VA
Inventor
Ju-Sung HUNG
Philip Lee
Amedeo Cappione
Original Assignee
Emd Millipore Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/436,992 external-priority patent/US9637715B2/en
Application filed by Emd Millipore Corp filed Critical Emd Millipore Corp
Publication of SG10201803961VA publication Critical patent/SG10201803961VA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5029—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0457—Moving fluids with specific forces or mechanical means specific forces passive flow or gravitation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1027—Determining speed or velocity of a particle

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Clinical Laboratory Science (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

CELL CULTURE AND GRADIENT MIGRATION ASSAY METHODS AND DEVICES A number of novel improved microfluidic configurations and systems and methods of manufacture and operation for a microfluidic invasion assay system. (FIG. A-B)
SG10201803961VA 2012-04-01 2013-02-06 Cell culture and gradient migration assay methods and devices SG10201803961VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13/436,992 US9637715B2 (en) 2005-07-07 2012-04-01 Cell culture and invasion assay method and system
US201361761227P 2013-02-05 2013-02-05

Publications (1)

Publication Number Publication Date
SG10201803961VA true SG10201803961VA (en) 2018-07-30

Family

ID=49300898

Family Applications (3)

Application Number Title Priority Date Filing Date
SG10201803961VA SG10201803961VA (en) 2012-04-01 2013-02-06 Cell culture and gradient migration assay methods and devices
SG10201607963SA SG10201607963SA (en) 2012-04-01 2013-02-06 Cell culture and gradient migration assay method and devices
SG11201405210QA SG11201405210QA (en) 2012-04-01 2013-02-06 Cell culture and gradient migration assay methods and devices

Family Applications After (2)

Application Number Title Priority Date Filing Date
SG10201607963SA SG10201607963SA (en) 2012-04-01 2013-02-06 Cell culture and gradient migration assay method and devices
SG11201405210QA SG11201405210QA (en) 2012-04-01 2013-02-06 Cell culture and gradient migration assay methods and devices

Country Status (5)

Country Link
EP (1) EP2834649A4 (en)
JP (2) JP2015517804A (en)
CN (2) CN104412109A (en)
SG (3) SG10201803961VA (en)
WO (1) WO2013151616A1 (en)

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FR2992206B1 (en) * 2012-06-21 2014-07-18 Oreal COSMETIC COMPOSITION COMPRISING AN OIL, HYDROPHOBIC SILICA AEROGEL PARTICLES AND A HYDROCARBON RESIN
AU2014218723B2 (en) 2013-02-22 2019-12-05 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
CA2901940A1 (en) 2013-02-22 2014-08-28 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
US11130935B2 (en) 2014-02-25 2021-09-28 Kyoto University Microfluid device and three-dimensional microculture method for cell
TW202204596A (en) 2014-06-06 2022-02-01 美商健臻公司 Perfusion culturing methods and uses thereof
TWI776235B (en) 2014-06-09 2022-09-01 美商健臻公司 Seed train processes and uses thereof
CA3225136A1 (en) 2014-12-22 2016-06-30 Genzyme Corporation Methods of culturing a mammalian cell
CN105062879B (en) * 2015-07-26 2017-06-27 江苏大学附属医院 The multidirectional migration experiment device of cell
CN105062890A (en) * 2015-09-11 2015-11-18 温州梅塔光学科技有限公司 Single cell experiment device
NL2016404B1 (en) 2016-03-09 2017-09-26 Mimetas B V Double tubular structures.
AU2017286096B2 (en) 2016-06-15 2022-01-20 Mimetas B.V. Cell culture device and methods
CN109475864B (en) * 2016-06-29 2022-03-04 美天施生物科技有限两合公司 Multi-stage disposable cartridge for biological samples
WO2018234163A1 (en) * 2017-06-16 2018-12-27 İni̇ti̇o Biyomedikal Mühendislik Danişmanlik Sanayi Ve Ticaret Limited Şirketi Method for determining cell migration and invasion
JP6812944B2 (en) * 2017-10-18 2021-01-13 株式会社島津製作所 Information management device for mass spectrometer
NL2020518B1 (en) 2018-03-02 2019-09-12 Mimetas B V Device and method for performing electrical measurements
CN108641931A (en) * 2018-04-04 2018-10-12 浙江大学 A kind of digitlization microarray organ chip and its application
CN111057639B (en) * 2019-11-29 2022-06-17 中国医科大学 Circulating tumor cell separation and enrichment system and method based on micro-fluidic chip
WO2022009663A1 (en) * 2020-07-06 2022-01-13 株式会社ニコン Migration ability calculation device, migration ability evaluation method, and computer program for causing computer to execute migration ability evaluation method
WO2022018743A1 (en) * 2020-07-22 2022-01-27 INDIAN INSTITUTE OF TECHNOLOGY MADRAS (IIT Madras) A system and method for simultaneous live-cell imaging and growing
CN112646713A (en) * 2020-12-25 2021-04-13 中国科学院广州生物医药与健康研究院 Chip for integrated tumor cell behavior experiment
NL2028424B1 (en) 2021-06-10 2022-12-20 Mimetas B V Method and apparatus for forming a microfluidic gel structure
CN113648287B (en) * 2021-07-30 2023-05-05 深圳华源再生医学有限公司 Cell loading device and preparation method thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004020341A2 (en) * 2002-08-27 2004-03-11 Vanderbilt University Bioreactors with substance injection capacity
KR20060127003A (en) * 2003-12-19 2006-12-11 유니버시티 오브 워터루 Cultured cell and method and apparatus for cell culture
US20080233607A1 (en) * 2004-11-11 2008-09-25 Hanry Yu Cell Culture Device
US9388374B2 (en) * 2005-07-07 2016-07-12 Emd Millipore Corporation Microfluidic cell culture systems
WO2009062095A1 (en) * 2007-11-07 2009-05-14 Bellbrook Labs, Llc Microfluidic device having stable static gradient for analyzing chemotaxis
US20130189671A1 (en) * 2010-08-19 2013-07-25 Jan H. Hoh Cell Culture System and Method of Use Thereof

Also Published As

Publication number Publication date
EP2834649A1 (en) 2015-02-11
CN104412109A (en) 2015-03-11
CN106399094A (en) 2017-02-15
SG10201607963SA (en) 2016-11-29
JP2015517804A (en) 2015-06-25
JP2017093467A (en) 2017-06-01
WO2013151616A1 (en) 2013-10-10
SG11201405210QA (en) 2014-09-26
EP2834649A4 (en) 2015-12-09

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