SG10201607963SA - Cell culture and gradient migration assay method and devices - Google Patents
Cell culture and gradient migration assay method and devicesInfo
- Publication number
- SG10201607963SA SG10201607963SA SG10201607963SA SG10201607963SA SG10201607963SA SG 10201607963S A SG10201607963S A SG 10201607963SA SG 10201607963S A SG10201607963S A SG 10201607963SA SG 10201607963S A SG10201607963S A SG 10201607963SA SG 10201607963S A SG10201607963S A SG 10201607963SA
- Authority
- SG
- Singapore
- Prior art keywords
- devices
- cell culture
- assay method
- migration assay
- gradient migration
- Prior art date
Links
- 238000004113 cell culture Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 238000010232 migration assay Methods 0.000 title 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1484—Electro-optical investigation, e.g. flow cytometers microstructural devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
- G01N33/5029—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0829—Multi-well plates; Microtitration plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0877—Flow chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0457—Moving fluids with specific forces or mechanical means specific forces passive flow or gravitation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G01N2015/1027—
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US13/436,992 US9637715B2 (en) | 2005-07-07 | 2012-04-01 | Cell culture and invasion assay method and system |
US201361761227P | 2013-02-05 | 2013-02-05 |
Publications (1)
Publication Number | Publication Date |
---|---|
SG10201607963SA true SG10201607963SA (en) | 2016-11-29 |
Family
ID=49300898
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG10201607963SA SG10201607963SA (en) | 2012-04-01 | 2013-02-06 | Cell culture and gradient migration assay method and devices |
SG11201405210QA SG11201405210QA (en) | 2012-04-01 | 2013-02-06 | Cell culture and gradient migration assay methods and devices |
SG10201803961VA SG10201803961VA (en) | 2012-04-01 | 2013-02-06 | Cell culture and gradient migration assay methods and devices |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11201405210QA SG11201405210QA (en) | 2012-04-01 | 2013-02-06 | Cell culture and gradient migration assay methods and devices |
SG10201803961VA SG10201803961VA (en) | 2012-04-01 | 2013-02-06 | Cell culture and gradient migration assay methods and devices |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP2834649A4 (en) |
JP (2) | JP2015517804A (en) |
CN (2) | CN106399094A (en) |
SG (3) | SG10201607963SA (en) |
WO (1) | WO2013151616A1 (en) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2992206B1 (en) * | 2012-06-21 | 2014-07-18 | Oreal | COSMETIC COMPOSITION COMPRISING AN OIL, HYDROPHOBIC SILICA AEROGEL PARTICLES AND A HYDROCARBON RESIN |
SG11201506339RA (en) | 2013-02-22 | 2015-09-29 | Genzyme Corp | Microcarrier perfusion culturing methods and uses thereof |
EP2958988A2 (en) | 2013-02-22 | 2015-12-30 | Genzyme Corporation | Microcarrier perfusion culturing methods and uses thereof |
EP3112450A4 (en) * | 2014-02-25 | 2017-11-08 | Kyoto University | Microfluid device and three-dimensional microculture method for cell |
TWI743024B (en) * | 2014-06-06 | 2021-10-21 | 美商健臻公司 | Perfusion culturing methods and uses thereof |
TWI707949B (en) | 2014-06-09 | 2020-10-21 | 美商健臻公司 | Seed train processes and uses thereof |
CN107567495A (en) | 2014-12-22 | 2018-01-09 | 建新公司 | The method for cultivating mammalian cell |
CN105062879B (en) * | 2015-07-26 | 2017-06-27 | 江苏大学附属医院 | The multidirectional migration experiment device of cell |
CN105062890A (en) * | 2015-09-11 | 2015-11-18 | 温州梅塔光学科技有限公司 | Single cell experiment device |
NL2016404B1 (en) | 2016-03-09 | 2017-09-26 | Mimetas B V | Double tubular structures. |
AU2017286096B2 (en) | 2016-06-15 | 2022-01-20 | Mimetas B.V. | Cell culture device and methods |
US11161111B2 (en) * | 2016-06-29 | 2021-11-02 | Miltenyi Biotec B. V. & Co. KG | Multilevel disposable cartridge for biological specimens |
WO2018234163A1 (en) * | 2017-06-16 | 2018-12-27 | İni̇ti̇o Biyomedikal Mühendislik Danişmanlik Sanayi Ve Ticaret Limited Şirketi | Method for determining cell migration and invasion |
JP6812944B2 (en) * | 2017-10-18 | 2021-01-13 | 株式会社島津製作所 | Information management device for mass spectrometer |
NL2020518B1 (en) | 2018-03-02 | 2019-09-12 | Mimetas B V | Device and method for performing electrical measurements |
CN108641931A (en) * | 2018-04-04 | 2018-10-12 | 浙江大学 | A kind of digitlization microarray organ chip and its application |
CN111057639B (en) * | 2019-11-29 | 2022-06-17 | 中国医科大学 | Circulating tumor cell separation and enrichment system and method based on micro-fluidic chip |
WO2022009663A1 (en) * | 2020-07-06 | 2022-01-13 | 株式会社ニコン | Migration ability calculation device, migration ability evaluation method, and computer program for causing computer to execute migration ability evaluation method |
WO2022018743A1 (en) * | 2020-07-22 | 2022-01-27 | INDIAN INSTITUTE OF TECHNOLOGY MADRAS (IIT Madras) | A system and method for simultaneous live-cell imaging and growing |
CN112646713A (en) * | 2020-12-25 | 2021-04-13 | 中国科学院广州生物医药与健康研究院 | Chip for integrated tumor cell behavior experiment |
NL2028424B1 (en) | 2021-06-10 | 2022-12-20 | Mimetas B V | Method and apparatus for forming a microfluidic gel structure |
CN113648287B (en) * | 2021-07-30 | 2023-05-05 | 深圳华源再生医学有限公司 | Cell loading device and preparation method thereof |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8129179B2 (en) * | 2002-08-27 | 2012-03-06 | Vanderbilt University | Bioreactors with an array of chambers and a common feed line |
WO2005059088A1 (en) * | 2003-12-19 | 2005-06-30 | University Of Waterloo | Cultured cell and method and apparatus for cell culture |
CA2586400A1 (en) * | 2004-11-11 | 2006-05-18 | Agency For Science, Technology And Research | Cell culture device |
US9388374B2 (en) * | 2005-07-07 | 2016-07-12 | Emd Millipore Corporation | Microfluidic cell culture systems |
WO2009062095A1 (en) * | 2007-11-07 | 2009-05-14 | Bellbrook Labs, Llc | Microfluidic device having stable static gradient for analyzing chemotaxis |
US20130189671A1 (en) * | 2010-08-19 | 2013-07-25 | Jan H. Hoh | Cell Culture System and Method of Use Thereof |
-
2013
- 2013-02-06 CN CN201610259619.3A patent/CN106399094A/en active Pending
- 2013-02-06 CN CN201380018324.1A patent/CN104412109A/en active Pending
- 2013-02-06 SG SG10201607963SA patent/SG10201607963SA/en unknown
- 2013-02-06 SG SG11201405210QA patent/SG11201405210QA/en unknown
- 2013-02-06 SG SG10201803961VA patent/SG10201803961VA/en unknown
- 2013-02-06 EP EP13772405.0A patent/EP2834649A4/en not_active Withdrawn
- 2013-02-06 JP JP2015503203A patent/JP2015517804A/en active Pending
- 2013-02-06 WO PCT/US2013/024999 patent/WO2013151616A1/en active Application Filing
-
2017
- 2017-02-06 JP JP2017019804A patent/JP2017093467A/en active Pending
Also Published As
Publication number | Publication date |
---|---|
SG11201405210QA (en) | 2014-09-26 |
EP2834649A1 (en) | 2015-02-11 |
WO2013151616A1 (en) | 2013-10-10 |
CN104412109A (en) | 2015-03-11 |
SG10201803961VA (en) | 2018-07-30 |
CN106399094A (en) | 2017-02-15 |
JP2015517804A (en) | 2015-06-25 |
JP2017093467A (en) | 2017-06-01 |
EP2834649A4 (en) | 2015-12-09 |
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