SG11201405210QA - Cell culture and gradient migration assay methods and devices - Google Patents

Cell culture and gradient migration assay methods and devices

Info

Publication number
SG11201405210QA
SG11201405210QA SG11201405210QA SG11201405210QA SG11201405210QA SG 11201405210Q A SG11201405210Q A SG 11201405210QA SG 11201405210Q A SG11201405210Q A SG 11201405210QA SG 11201405210Q A SG11201405210Q A SG 11201405210QA SG 11201405210Q A SG11201405210Q A SG 11201405210QA
Authority
SG
Singapore
Prior art keywords
devices
cell culture
assay methods
migration assay
gradient migration
Prior art date
Application number
SG11201405210QA
Inventor
Ju-Sung Paul Hung
Philip J Lee
Amedeo J Cappione
Original Assignee
Emd Millipore Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/436,992 external-priority patent/US9637715B2/en
Application filed by Emd Millipore Corp filed Critical Emd Millipore Corp
Publication of SG11201405210QA publication Critical patent/SG11201405210QA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5029—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0457—Moving fluids with specific forces or mechanical means specific forces passive flow or gravitation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1027—Determining speed or velocity of a particle

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SG11201405210QA 2012-04-01 2013-02-06 Cell culture and gradient migration assay methods and devices SG11201405210QA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13/436,992 US9637715B2 (en) 2005-07-07 2012-04-01 Cell culture and invasion assay method and system
US201361761227P 2013-02-05 2013-02-05
PCT/US2013/024999 WO2013151616A1 (en) 2012-04-01 2013-02-06 Cell culture and gradient migration assay methods and devices

Publications (1)

Publication Number Publication Date
SG11201405210QA true SG11201405210QA (en) 2014-09-26

Family

ID=49300898

Family Applications (3)

Application Number Title Priority Date Filing Date
SG10201607963SA SG10201607963SA (en) 2012-04-01 2013-02-06 Cell culture and gradient migration assay method and devices
SG10201803961VA SG10201803961VA (en) 2012-04-01 2013-02-06 Cell culture and gradient migration assay methods and devices
SG11201405210QA SG11201405210QA (en) 2012-04-01 2013-02-06 Cell culture and gradient migration assay methods and devices

Family Applications Before (2)

Application Number Title Priority Date Filing Date
SG10201607963SA SG10201607963SA (en) 2012-04-01 2013-02-06 Cell culture and gradient migration assay method and devices
SG10201803961VA SG10201803961VA (en) 2012-04-01 2013-02-06 Cell culture and gradient migration assay methods and devices

Country Status (5)

Country Link
EP (1) EP2834649A4 (en)
JP (2) JP2015517804A (en)
CN (2) CN106399094A (en)
SG (3) SG10201607963SA (en)
WO (1) WO2013151616A1 (en)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2992206B1 (en) * 2012-06-21 2014-07-18 Oreal COSMETIC COMPOSITION COMPRISING AN OIL, HYDROPHOBIC SILICA AEROGEL PARTICLES AND A HYDROCARBON RESIN
HUE042094T2 (en) 2013-02-22 2019-06-28 Genzyme Corp Microcarrier perfusion culturing methods and uses thereof
AU2014218723B2 (en) 2013-02-22 2019-12-05 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
WO2015129673A1 (en) * 2014-02-25 2015-09-03 国立大学法人京都大学 Microfluid device and three-dimensional microculture method for cell
TWI743024B (en) 2014-06-06 2021-10-21 美商健臻公司 Perfusion culturing methods and uses thereof
TWI776235B (en) 2014-06-09 2022-09-01 美商健臻公司 Seed train processes and uses thereof
CA2971861C (en) 2014-12-22 2024-02-13 Genzyme Corporation Methods of culturing a mammalian cell
CN105062879B (en) * 2015-07-26 2017-06-27 江苏大学附属医院 The multidirectional migration experiment device of cell
CN105062890A (en) * 2015-09-11 2015-11-18 温州梅塔光学科技有限公司 Single cell experiment device
NL2016404B1 (en) 2016-03-09 2017-09-26 Mimetas B V Double tubular structures.
JP6967535B2 (en) 2016-06-15 2021-11-17 ミメタス ビー．ブイ． Cell culture equipment and method
WO2018001767A1 (en) * 2016-06-29 2018-01-04 Miltenyi Biotec Gmbh Multilevel disposable cartridge for biological specimens
WO2018234163A1 (en) * 2017-06-16 2018-12-27 İni̇ti̇o Biyomedikal Mühendislik Danişmanlik Sanayi Ve Ticaret Limited Şirketi Method for determining cell migration and invasion
JP6812944B2 (en) * 2017-10-18 2021-01-13 株式会社島津製作所 Information management device for mass spectrometer
NL2020518B1 (en) 2018-03-02 2019-09-12 Mimetas B V Device and method for performing electrical measurements
CN108641931A (en) * 2018-04-04 2018-10-12 浙江大学 A kind of digitlization microarray organ chip and its application
CN111057639B (en) * 2019-11-29 2022-06-17 中国医科大学 Circulating tumor cell separation and enrichment system and method based on micro-fluidic chip
WO2022009663A1 (en) * 2020-07-06 2022-01-13 株式会社ニコン Migration ability calculation device, migration ability evaluation method, and computer program for causing computer to execute migration ability evaluation method
WO2022018743A1 (en) * 2020-07-22 2022-01-27 INDIAN INSTITUTE OF TECHNOLOGY MADRAS (IIT Madras) A system and method for simultaneous live-cell imaging and growing
CN112646713A (en) * 2020-12-25 2021-04-13 中国科学院广州生物医药与健康研究院 Chip for integrated tumor cell behavior experiment
NL2028424B1 (en) 2021-06-10 2022-12-20 Mimetas B V Method and apparatus for forming a microfluidic gel structure
CN113648287B (en) * 2021-07-30 2023-05-05 深圳华源再生医学有限公司 Cell loading device and preparation method thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004020590A2 (en) * 2002-08-27 2004-03-11 Vanderbilt University Bioreactors with multiple chambers
AU2004299530A1 (en) * 2003-12-19 2005-06-30 University Of Waterloo Cultured cell and method and apparatus for cell culture
WO2006052223A1 (en) * 2004-11-11 2006-05-18 Agency For Science, Technology And Research Cell culture device
US9388374B2 (en) * 2005-07-07 2016-07-12 Emd Millipore Corporation Microfluidic cell culture systems
WO2009062095A1 (en) * 2007-11-07 2009-05-14 Bellbrook Labs, Llc Microfluidic device having stable static gradient for analyzing chemotaxis
WO2012024646A2 (en) * 2010-08-19 2012-02-23 The Johns Hopkins University Cell culture system and method of use thereof

Also Published As

Publication number Publication date
CN104412109A (en) 2015-03-11
EP2834649A1 (en) 2015-02-11
EP2834649A4 (en) 2015-12-09
CN106399094A (en) 2017-02-15
JP2017093467A (en) 2017-06-01
WO2013151616A1 (en) 2013-10-10
SG10201803961VA (en) 2018-07-30
JP2015517804A (en) 2015-06-25
SG10201607963SA (en) 2016-11-29

Similar Documents

Publication Publication Date Title
SG11201405210QA (en) Cell culture and gradient migration assay methods and devices
HK1251033A1 (en) Whole cell assays and methods
HK1210494A1 (en) Devices and methods for culture of cells
SG10201604400RA (en) Micro-incubation systems for microfluidic cell culture and methods
EP2902475A4 (en) Cell-culturing vessel and cell-culturing device using same
EP2968549A4 (en) Methods of cell culture
EP2843036A4 (en) Cell culture system and cell culture method
EP2971014A4 (en) Methods of cell culture
EP2971040A4 (en) Methods of cell culture
HK1207121A1 (en) Cell culturing device
SG11201503579RA (en) Cell culture med
HK1214412A1 (en) Electrochemical bioreactor module and methods of using the same
EP2860240A4 (en) Cell culture apparatus and cell culture method using the same
HK1214292A1 (en) Perfusion culturing methods and uses thereof
SG10201700670WA (en) Cell culture
EP2824182A4 (en) Method for stimulating t cell and use thereof
EP2975110A4 (en) Cell culture device
SG11201502497RA (en) Sebocyte cell culturing and methods of use
EP2725097A4 (en) Cell culture device and cell culture method
EP2900803A4 (en) Devices and methods for single cell analysis
PT2861713T (en) Cell culture method and system
KR102296181B9 (en) Cell culture system using complex stimulus
EP2887495A4 (en) Cell balancing device and cell balancing method
GB201211873D0 (en) Cell culture
EP2924110A4 (en) Cell culture device and cell culture method