CA3151149C - Genetically engineered bacterium comprising energy-generating fermentation pathway - Google Patents
Genetically engineered bacterium comprising energy-generating fermentation pathway Download PDFInfo
- Publication number
- CA3151149C CA3151149C CA3151149A CA3151149A CA3151149C CA 3151149 C CA3151149 C CA 3151149C CA 3151149 A CA3151149 A CA 3151149A CA 3151149 A CA3151149 A CA 3151149A CA 3151149 C CA3151149 C CA 3151149C
- Authority
- CA
- Canada
- Prior art keywords
- clostridium
- coa
- microorganism
- aldehyde
- ptb
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000037361 pathway Effects 0.000 title claims abstract description 86
- 241000894006 Bacteria Species 0.000 title claims abstract description 30
- 238000000855 fermentation Methods 0.000 title abstract description 47
- 230000004151 fermentation Effects 0.000 title abstract description 47
- 239000000758 substrate Substances 0.000 claims abstract description 166
- 108700024126 Butyrate kinases Proteins 0.000 claims abstract description 132
- 108700024327 Phosphate butyryltransferases Proteins 0.000 claims abstract description 126
- 238000000034 method Methods 0.000 claims abstract description 54
- 244000005700 microbiome Species 0.000 claims description 332
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 271
- 241001656809 Clostridium autoethanogenum Species 0.000 claims description 237
- 102000004190 Enzymes Human genes 0.000 claims description 215
- 108090000790 Enzymes Proteins 0.000 claims description 215
- 238000004519 manufacturing process Methods 0.000 claims description 198
- 241000588724 Escherichia coli Species 0.000 claims description 111
- 108020002982 thioesterase Proteins 0.000 claims description 104
- 241001611023 Clostridium ragsdalei Species 0.000 claims description 102
- 102000005488 Thioesterase Human genes 0.000 claims description 100
- 241000186566 Clostridium ljungdahlii Species 0.000 claims description 82
- 239000002243 precursor Substances 0.000 claims description 79
- 241000193403 Clostridium Species 0.000 claims description 53
- ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 claims description 46
- 150000007523 nucleic acids Chemical class 0.000 claims description 46
- 108020004707 nucleic acids Proteins 0.000 claims description 44
- 102000039446 nucleic acids Human genes 0.000 claims description 44
- 108010021809 Alcohol dehydrogenase Proteins 0.000 claims description 33
- 241000193454 Clostridium beijerinckii Species 0.000 claims description 33
- 102000007698 Alcohol dehydrogenase Human genes 0.000 claims description 32
- 230000035772 mutation Effects 0.000 claims description 31
- 229910052799 carbon Inorganic materials 0.000 claims description 27
- 239000002253 acid Substances 0.000 claims description 22
- 108060008225 Thiolase Proteins 0.000 claims description 21
- 102000002932 Thiolase Human genes 0.000 claims description 20
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 17
- 229910002091 carbon monoxide Inorganic materials 0.000 claims description 16
- 238000012258 culturing Methods 0.000 claims description 16
- 241000186570 Clostridium kluyveri Species 0.000 claims description 13
- 241001528539 Cupriavidus necator Species 0.000 claims description 13
- 108010023922 Enoyl-CoA hydratase Proteins 0.000 claims description 12
- 102000011426 Enoyl-CoA hydratase Human genes 0.000 claims description 12
- 108091022873 acetoacetate decarboxylase Proteins 0.000 claims description 12
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical group CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 claims description 12
- 101710088194 Dehydrogenase Proteins 0.000 claims description 11
- 230000001419 dependent effect Effects 0.000 claims description 11
- 241001508458 Clostridium saccharoperbutylacetonicum Species 0.000 claims description 7
- KBPLFHHGFOOTCA-UHFFFAOYSA-N 1-Octanol Chemical compound CCCCCCCCO KBPLFHHGFOOTCA-UHFFFAOYSA-N 0.000 claims description 6
- ZSIAUFGUXNUGDI-UHFFFAOYSA-N hexan-1-ol Chemical compound CCCCCCO ZSIAUFGUXNUGDI-UHFFFAOYSA-N 0.000 claims description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 5
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 5
- 102100024639 Short-chain specific acyl-CoA dehydrogenase, mitochondrial Human genes 0.000 claims description 5
- 241000193171 Clostridium butyricum Species 0.000 claims description 4
- 244000057717 Streptococcus lactis Species 0.000 claims description 4
- 235000014897 Streptococcus lactis Nutrition 0.000 claims description 4
- 241000186339 Thermoanaerobacter Species 0.000 claims description 4
- 108010068197 Butyryl-CoA Dehydrogenase Proteins 0.000 claims description 3
- 241000193169 Clostridium cellulovorans Species 0.000 claims description 3
- 241001468161 Acetobacterium Species 0.000 claims description 2
- 244000063299 Bacillus subtilis Species 0.000 claims description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 2
- 241000193163 Clostridioides difficile Species 0.000 claims description 2
- 241000186226 Corynebacterium glutamicum Species 0.000 claims description 2
- 241000588749 Klebsiella oxytoca Species 0.000 claims description 2
- 201000008225 Klebsiella pneumonia Diseases 0.000 claims description 2
- 241000588747 Klebsiella pneumoniae Species 0.000 claims description 2
- 241000933069 Lachnoclostridium phytofermentans Species 0.000 claims description 2
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 2
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 2
- 241000589308 Methylobacterium extorquens Species 0.000 claims description 2
- 241000178985 Moorella Species 0.000 claims description 2
- 206010035717 Pneumonia klebsiella Diseases 0.000 claims description 2
- 241000589776 Pseudomonas putida Species 0.000 claims description 2
- 241000193448 Ruminiclostridium thermocellum Species 0.000 claims description 2
- 241000588902 Zymomonas mobilis Species 0.000 claims description 2
- 241000193453 [Clostridium] cellulolyticum Species 0.000 claims description 2
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 2
- 239000004606 Fillers/Extenders Substances 0.000 claims 3
- 150000000185 1,3-diols Chemical class 0.000 claims 2
- 102000008170 Aldehyde Oxidoreductases Human genes 0.000 claims 2
- 108010060441 Aldehyde Oxidoreductases Proteins 0.000 claims 2
- QPMJENKZJUFOON-PLNGDYQASA-N ethyl (z)-3-chloro-2-cyano-4,4,4-trifluorobut-2-enoate Chemical compound CCOC(=O)C(\C#N)=C(/Cl)C(F)(F)F QPMJENKZJUFOON-PLNGDYQASA-N 0.000 claims 2
- 150000007970 thio esters Chemical class 0.000 claims 2
- 241000037909 Alkalibaculum Species 0.000 claims 1
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 241001202853 Blautia Species 0.000 claims 1
- 241000186581 Clostridium novyi Species 0.000 claims 1
- 108010079426 Electron-Transferring Flavoproteins Proteins 0.000 claims 1
- 102000012737 Electron-Transferring Flavoproteins Human genes 0.000 claims 1
- 241000186394 Eubacterium Species 0.000 claims 1
- 241000178986 Oxobacter Species 0.000 claims 1
- 241000204388 Sporomusa Species 0.000 claims 1
- 239000000543 intermediate Substances 0.000 abstract description 10
- 108010074122 Ferredoxins Proteins 0.000 description 350
- 150000001299 aldehydes Chemical class 0.000 description 344
- 102000004316 Oxidoreductases Human genes 0.000 description 332
- 108090000854 Oxidoreductases Proteins 0.000 description 332
- 239000000047 product Substances 0.000 description 146
- 230000000694 effects Effects 0.000 description 141
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 132
- 238000006243 chemical reaction Methods 0.000 description 121
- WHBMMWSBFZVSSR-UHFFFAOYSA-N R3HBA Natural products CC(O)CC(O)=O WHBMMWSBFZVSSR-UHFFFAOYSA-N 0.000 description 98
- WHBMMWSBFZVSSR-UHFFFAOYSA-M 3-hydroxybutyrate Chemical compound CC(O)CC([O-])=O WHBMMWSBFZVSSR-UHFFFAOYSA-M 0.000 description 97
- 239000013612 plasmid Substances 0.000 description 93
- 108090000623 proteins and genes Proteins 0.000 description 87
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 84
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 83
- VQTUBCCKSQIDNK-UHFFFAOYSA-N Isobutene Chemical group CC(C)=C VQTUBCCKSQIDNK-UHFFFAOYSA-N 0.000 description 51
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 50
- WNLRTRBMVRJNCN-UHFFFAOYSA-N adipic acid Chemical compound OC(=O)CCCCC(O)=O WNLRTRBMVRJNCN-UHFFFAOYSA-N 0.000 description 46
- 241000193464 Clostridium sp. Species 0.000 description 45
- 239000007789 gas Substances 0.000 description 43
- OJFDKHTZOUZBOS-CITAKDKDSA-N acetoacetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 OJFDKHTZOUZBOS-CITAKDKDSA-N 0.000 description 41
- 230000012010 growth Effects 0.000 description 40
- 229920001791 ((R)-3-Hydroxybutanoyl)(n-2) Polymers 0.000 description 38
- QHHKKMYHDBRONY-RMNRSTNRSA-N 3-hydroxybutanoyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC(O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 QHHKKMYHDBRONY-RMNRSTNRSA-N 0.000 description 38
- 241000193401 Clostridium acetobutylicum Species 0.000 description 36
- 102000004169 proteins and genes Human genes 0.000 description 35
- 101150096860 thlA gene Proteins 0.000 description 35
- FFVUICCDNWZCRC-ZSJPKINUSA-N 2-hydroxyisobutanoyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C(C)(C)O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 FFVUICCDNWZCRC-ZSJPKINUSA-N 0.000 description 34
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 34
- 230000014509 gene expression Effects 0.000 description 34
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 28
- 108010084715 isopropanol dehydrogenase (NADP) Proteins 0.000 description 28
- AXPZIVKEZRHGAS-UHFFFAOYSA-N 3-benzyl-5-[(2-nitrophenoxy)methyl]oxolan-2-one Chemical compound [O-][N+](=O)C1=CC=CC=C1OCC1OC(=O)C(CC=2C=CC=CC=2)C1 AXPZIVKEZRHGAS-UHFFFAOYSA-N 0.000 description 26
- BWLBGMIXKSTLSX-UHFFFAOYSA-M 2-hydroxyisobutyrate Chemical compound CC(C)(O)C([O-])=O BWLBGMIXKSTLSX-UHFFFAOYSA-M 0.000 description 25
- WDJHALXBUFZDSR-UHFFFAOYSA-M acetoacetate Chemical compound CC(=O)CC([O-])=O WDJHALXBUFZDSR-UHFFFAOYSA-M 0.000 description 24
- 229910002092 carbon dioxide Inorganic materials 0.000 description 24
- 241000193155 Clostridium botulinum Species 0.000 description 23
- 239000001361 adipic acid Substances 0.000 description 23
- 235000011037 adipic acid Nutrition 0.000 description 23
- 230000015572 biosynthetic process Effects 0.000 description 23
- 230000002018 overexpression Effects 0.000 description 23
- 241000894007 species Species 0.000 description 23
- BWLBGMIXKSTLSX-UHFFFAOYSA-N 2-hydroxyisobutyric acid Chemical compound CC(C)(O)C(O)=O BWLBGMIXKSTLSX-UHFFFAOYSA-N 0.000 description 20
- 230000001965 increasing effect Effects 0.000 description 20
- 239000000203 mixture Substances 0.000 description 20
- 101150110984 phaB gene Proteins 0.000 description 20
- 108090000124 3-hydroxybutyrate dehydrogenases Proteins 0.000 description 19
- 108010055682 3-hydroxybutyryl-CoA dehydrogenase Proteins 0.000 description 19
- 101100297400 Rhizobium meliloti (strain 1021) phaAB gene Proteins 0.000 description 19
- 101100280476 Streptococcus pneumoniae (strain ATCC BAA-255 / R6) fabM gene Proteins 0.000 description 19
- 238000003556 assay Methods 0.000 description 19
- 102000034279 3-hydroxybutyrate dehydrogenases Human genes 0.000 description 18
- 108091005461 Nucleic proteins Proteins 0.000 description 18
- 230000002829 reductive effect Effects 0.000 description 18
- 102100039894 Hemoglobin subunit delta Human genes 0.000 description 17
- 101150015366 budA gene Proteins 0.000 description 17
- 238000002474 experimental method Methods 0.000 description 17
- 238000010586 diagram Methods 0.000 description 16
- 230000002441 reversible effect Effects 0.000 description 16
- 108090000489 Carboxy-Lyases Proteins 0.000 description 15
- 101150006589 adc gene Proteins 0.000 description 15
- 101150056596 azin2 gene Proteins 0.000 description 15
- 230000009483 enzymatic pathway Effects 0.000 description 15
- 230000007062 hydrolysis Effects 0.000 description 15
- 238000006460 hydrolysis reaction Methods 0.000 description 15
- MXLMTQWGSQIYOW-UHFFFAOYSA-N 3-methyl-2-butanol Chemical compound CC(C)C(C)O MXLMTQWGSQIYOW-UHFFFAOYSA-N 0.000 description 14
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 14
- 102000004031 Carboxy-Lyases Human genes 0.000 description 14
- 101001028272 Escherichia coli (strain K12) Long-chain acyl-CoA thioesterase FadM Proteins 0.000 description 14
- CRFNGMNYKDXRTN-CITAKDKDSA-N butyryl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CCC)O[C@H]1N1C2=NC=NC(N)=C2N=C1 CRFNGMNYKDXRTN-CITAKDKDSA-N 0.000 description 14
- 238000010367 cloning Methods 0.000 description 14
- 230000021615 conjugation Effects 0.000 description 14
- -1 meaBhcmA Proteins 0.000 description 14
- 239000002609 medium Substances 0.000 description 14
- AFENDNXGAFYKQO-VKHMYHEASA-N (S)-2-hydroxybutyric acid Chemical compound CC[C@H](O)C(O)=O AFENDNXGAFYKQO-VKHMYHEASA-N 0.000 description 13
- 108020004414 DNA Proteins 0.000 description 13
- 241000904817 Lachnospiraceae bacterium Species 0.000 description 13
- OWBTYPJTUOEWEK-UHFFFAOYSA-N butane-2,3-diol Chemical compound CC(O)C(C)O OWBTYPJTUOEWEK-UHFFFAOYSA-N 0.000 description 13
- 238000001294 liquid chromatography-tandem mass spectrometry Methods 0.000 description 13
- GWYFCOCPABKNJV-UHFFFAOYSA-M 3-Methylbutanoic acid Natural products CC(C)CC([O-])=O GWYFCOCPABKNJV-UHFFFAOYSA-M 0.000 description 12
- KAKZBPTYRLMSJV-UHFFFAOYSA-N Butadiene Chemical compound C=CC=C KAKZBPTYRLMSJV-UHFFFAOYSA-N 0.000 description 12
- ZTQSAGDEMFDKMZ-UHFFFAOYSA-N Butyraldehyde Chemical compound CCCC=O ZTQSAGDEMFDKMZ-UHFFFAOYSA-N 0.000 description 12
- 101000693728 Homo sapiens S-acyl fatty acid synthase thioesterase, medium chain Proteins 0.000 description 12
- 102100025541 S-acyl fatty acid synthase thioesterase, medium chain Human genes 0.000 description 12
- 238000004458 analytical method Methods 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 12
- GWYFCOCPABKNJV-UHFFFAOYSA-N isovaleric acid Chemical compound CC(C)CC(O)=O GWYFCOCPABKNJV-UHFFFAOYSA-N 0.000 description 12
- WCASXYBKJHWFMY-NSCUHMNNSA-N 2-Buten-1-ol Chemical compound C\C=C\CO WCASXYBKJHWFMY-NSCUHMNNSA-N 0.000 description 11
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 11
- 241000193468 Clostridium perfringens Species 0.000 description 11
- 102000004357 Transferases Human genes 0.000 description 11
- 108090000992 Transferases Proteins 0.000 description 11
- AVIYEYCFMVPYST-UHFFFAOYSA-N hexane-1,3-diol Chemical compound CCCC(O)CCO AVIYEYCFMVPYST-UHFFFAOYSA-N 0.000 description 11
- 239000013598 vector Substances 0.000 description 11
- PEVZKILCBDEOBT-CITAKDKDSA-N 3-hydroxyisovaleryl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC(C)(O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 PEVZKILCBDEOBT-CITAKDKDSA-N 0.000 description 10
- GTLLKMCVRPVGBK-UHFFFAOYSA-N 3-methylbutaneperoxoic acid Chemical compound CC(C)CC(=O)OO GTLLKMCVRPVGBK-UHFFFAOYSA-N 0.000 description 10
- 101150101337 Bdh2 gene Proteins 0.000 description 10
- 239000002028 Biomass Substances 0.000 description 10
- FERIUCNNQQJTOY-UHFFFAOYSA-M Butyrate Chemical compound CCCC([O-])=O FERIUCNNQQJTOY-UHFFFAOYSA-M 0.000 description 10
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 10
- 229910000831 Steel Inorganic materials 0.000 description 10
- 230000000789 acetogenic effect Effects 0.000 description 10
- 150000007513 acids Chemical class 0.000 description 10
- JSHMCUNOMIZJDJ-UHFFFAOYSA-N butanoyl dihydrogen phosphate Chemical compound CCCC(=O)OP(O)(O)=O JSHMCUNOMIZJDJ-UHFFFAOYSA-N 0.000 description 10
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 10
- 239000012071 phase Substances 0.000 description 10
- 239000010959 steel Substances 0.000 description 10
- 230000004152 substrate-level phosphorylation Effects 0.000 description 10
- QHHKKMYHDBRONY-WZZMXTMRSA-N (R)-3-hydroxybutanoyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C[C@H](O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 QHHKKMYHDBRONY-WZZMXTMRSA-N 0.000 description 9
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 9
- 101100119095 Enterococcus faecalis (strain ATCC 700802 / V583) ermB gene Proteins 0.000 description 9
- 108010000775 Hydroxymethylglutaryl-CoA synthase Proteins 0.000 description 9
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 9
- 238000003786 synthesis reaction Methods 0.000 description 9
- 108020004705 Codon Proteins 0.000 description 8
- 241000878020 Haloterrigena turkmenica Species 0.000 description 8
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 8
- 108700023175 Phosphate acetyltransferases Proteins 0.000 description 8
- 239000006227 byproduct Substances 0.000 description 8
- 238000012217 deletion Methods 0.000 description 8
- 230000037430 deletion Effects 0.000 description 8
- 239000001257 hydrogen Substances 0.000 description 8
- 229910052739 hydrogen Inorganic materials 0.000 description 8
- 239000002207 metabolite Substances 0.000 description 8
- 230000003647 oxidation Effects 0.000 description 8
- 238000007254 oxidation reaction Methods 0.000 description 8
- 101150108780 pta gene Proteins 0.000 description 8
- 239000006228 supernatant Substances 0.000 description 8
- WHBMMWSBFZVSSR-GSVOUGTGSA-M (R)-3-hydroxybutyrate Chemical compound C[C@@H](O)CC([O-])=O WHBMMWSBFZVSSR-GSVOUGTGSA-M 0.000 description 7
- AXFYFNCPONWUHW-UHFFFAOYSA-N 3-hydroxyisovaleric acid Chemical compound CC(C)(O)CC(O)=O AXFYFNCPONWUHW-UHFFFAOYSA-N 0.000 description 7
- 241000928018 Caldivirga maquilingensis IC-167 Species 0.000 description 7
- 241001675829 Ferroglobus placidus DSM 10642 Species 0.000 description 7
- 102100028889 Hydroxymethylglutaryl-CoA synthase, mitochondrial Human genes 0.000 description 7
- 238000005119 centrifugation Methods 0.000 description 7
- AGOYDEPGAOXOCK-KCBOHYOISA-N clarithromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@](C)([C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)OC)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 AGOYDEPGAOXOCK-KCBOHYOISA-N 0.000 description 7
- 229960002626 clarithromycin Drugs 0.000 description 7
- 150000002009 diols Chemical class 0.000 description 7
- 230000006870 function Effects 0.000 description 7
- 235000000346 sugar Nutrition 0.000 description 7
- 238000011144 upstream manufacturing Methods 0.000 description 7
- BTANRVKWQNVYAZ-UHFFFAOYSA-N 2-butanol Substances CCC(C)O BTANRVKWQNVYAZ-UHFFFAOYSA-N 0.000 description 6
- MSXVEPNJUHWQHW-UHFFFAOYSA-N 2-methylbutan-2-ol Chemical compound CCC(C)(C)O MSXVEPNJUHWQHW-UHFFFAOYSA-N 0.000 description 6
- 108030005660 3-hydroxybutyryl-CoA dehydratases Proteins 0.000 description 6
- YGHRJJRRZDOVPD-UHFFFAOYSA-N 3-methylbutanal Chemical compound CC(C)CC=O YGHRJJRRZDOVPD-UHFFFAOYSA-N 0.000 description 6
- 108010092060 Acetate kinase Proteins 0.000 description 6
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 6
- 241000643378 Candidatus Korarchaeum Species 0.000 description 6
- 241000359383 Desulfurococcus amylolyticus Species 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 241001417618 Natronobacterium gregoryi SP2 Species 0.000 description 6
- 108091034117 Oligonucleotide Proteins 0.000 description 6
- 241001467519 Pyrococcus sp. Species 0.000 description 6
- 241000047166 Thermococcus sibiricus MM 739 Species 0.000 description 6
- 241001495444 Thermococcus sp. Species 0.000 description 6
- 101710081312 Trans-2-enoyl-CoA reductase Proteins 0.000 description 6
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 6
- YRKCREAYFQTBPV-UHFFFAOYSA-N acetylacetone Chemical compound CC(=O)CC(C)=O YRKCREAYFQTBPV-UHFFFAOYSA-N 0.000 description 6
- 150000001298 alcohols Chemical class 0.000 description 6
- 230000003321 amplification Effects 0.000 description 6
- 230000001651 autotrophic effect Effects 0.000 description 6
- 108010031234 carbon monoxide dehydrogenase Proteins 0.000 description 6
- 238000012824 chemical production Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 239000012634 fragment Substances 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 241001618048 halophilic archaeon DL31 Species 0.000 description 6
- 238000001727 in vivo Methods 0.000 description 6
- 230000037353 metabolic pathway Effects 0.000 description 6
- 238000010369 molecular cloning Methods 0.000 description 6
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 6
- 238000003199 nucleic acid amplification method Methods 0.000 description 6
- 230000009467 reduction Effects 0.000 description 6
- 229930101283 tetracycline Natural products 0.000 description 6
- HGBOYTHUEUWSSQ-UHFFFAOYSA-N valeric aldehyde Natural products CCCCC=O HGBOYTHUEUWSSQ-UHFFFAOYSA-N 0.000 description 6
- PMWATMXOQQZNBX-DKBZLLMOSA-N 2-methylcrotonoyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C(/C)=C/C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 PMWATMXOQQZNBX-DKBZLLMOSA-N 0.000 description 5
- 241000114861 Aciduliprofundum boonei T469 Species 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 5
- 241001055473 Archaeoglobus sulfaticallidus Species 0.000 description 5
- 241001110912 Clostridium beijerinckii NCIMB 8052 Species 0.000 description 5
- 241000193469 Clostridium pasteurianum Species 0.000 description 5
- 241000193449 Clostridium tetani Species 0.000 description 5
- 102000004157 Hydrolases Human genes 0.000 description 5
- 108090000604 Hydrolases Proteins 0.000 description 5
- 108090000769 Isomerases Proteins 0.000 description 5
- 102000004195 Isomerases Human genes 0.000 description 5
- 238000005481 NMR spectroscopy Methods 0.000 description 5
- 108090000472 Phosphoenolpyruvate carboxykinase (ATP) Proteins 0.000 description 5
- 241000517244 Pyrobaculum arsenaticum Species 0.000 description 5
- 241000065785 Pyrobaculum oguniense TE7 Species 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 5
- 230000008878 coupling Effects 0.000 description 5
- 238000010168 coupling process Methods 0.000 description 5
- 238000005859 coupling reaction Methods 0.000 description 5
- KFWWCMJSYSSPSK-PAXLJYGASA-N crotonoyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)/C=C/C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 KFWWCMJSYSSPSK-PAXLJYGASA-N 0.000 description 5
- 230000003247 decreasing effect Effects 0.000 description 5
- 238000004817 gas chromatography Methods 0.000 description 5
- 238000002309 gasification Methods 0.000 description 5
- 238000003197 gene knockdown Methods 0.000 description 5
- 239000011521 glass Substances 0.000 description 5
- 238000004128 high performance liquid chromatography Methods 0.000 description 5
- 238000003780 insertion Methods 0.000 description 5
- 230000037431 insertion Effects 0.000 description 5
- 230000014759 maintenance of location Effects 0.000 description 5
- 230000007246 mechanism Effects 0.000 description 5
- 230000000813 microbial effect Effects 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 238000005457 optimization Methods 0.000 description 5
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 5
- 239000004926 polymethyl methacrylate Substances 0.000 description 5
- 230000009466 transformation Effects 0.000 description 5
- QHHKKMYHDBRONY-VKBDFPRVSA-N (S)-3-hydroxybutanoyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C[C@@H](O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 QHHKKMYHDBRONY-VKBDFPRVSA-N 0.000 description 4
- WHBMMWSBFZVSSR-VKHMYHEASA-N (S)-3-hydroxybutyric acid Chemical compound C[C@H](O)CC(O)=O WHBMMWSBFZVSSR-VKHMYHEASA-N 0.000 description 4
- KIUMMUBSPKGMOY-UHFFFAOYSA-N 3,3'-Dithiobis(6-nitrobenzoic acid) Chemical compound C1=C([N+]([O-])=O)C(C(=O)O)=CC(SSC=2C=C(C(=CC=2)[N+]([O-])=O)C(O)=O)=C1 KIUMMUBSPKGMOY-UHFFFAOYSA-N 0.000 description 4
- QHKABHOOEWYVLI-UHFFFAOYSA-N 3-methyl-2-oxobutanoic acid Chemical compound CC(C)C(=O)C(O)=O QHKABHOOEWYVLI-UHFFFAOYSA-N 0.000 description 4
- 101710090359 4-hydroxybenzoyl-CoA thioesterase Proteins 0.000 description 4
- 108010000700 Acetolactate synthase Proteins 0.000 description 4
- 241000601487 Acidilobus saccharovorans 345-15 Species 0.000 description 4
- 241001329407 Aciduliprofundum sp. Species 0.000 description 4
- 102100025854 Acyl-coenzyme A thioesterase 1 Human genes 0.000 description 4
- 101710175445 Acyl-coenzyme A thioesterase 1 Proteins 0.000 description 4
- 102100022714 Acyl-coenzyme A thioesterase 13 Human genes 0.000 description 4
- 241000276439 Archaeoglobus fulgidus DSM 4304 Species 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 241001144648 Caldisphaera lagunensis Species 0.000 description 4
- 241001112695 Clostridiales Species 0.000 description 4
- 241000904825 Clostridiales bacterium Species 0.000 description 4
- 108700040197 Enoyl-CoA Hydratase 2 Proteins 0.000 description 4
- 241001267419 Eubacterium sp. Species 0.000 description 4
- 241000085256 Fervidicoccus fontis Kam940 Species 0.000 description 4
- 241000164875 Firmicutes bacterium Species 0.000 description 4
- 241001398756 Halalkalicoccus jeotgali B3 Species 0.000 description 4
- 241001417305 Halopiger xanaduensis SH-6 Species 0.000 description 4
- 241001006888 Hyperthermus butylicus DSM 5456 Species 0.000 description 4
- 241000863422 Myxococcus xanthus Species 0.000 description 4
- 241000172870 Natrialba magadii ATCC 43099 Species 0.000 description 4
- 241000007221 Natronococcus occultus SP4 Species 0.000 description 4
- 241000765915 Natronomonas pharaonis DSM 2160 Species 0.000 description 4
- 229910019142 PO4 Inorganic materials 0.000 description 4
- 108010035473 Palmitoyl-CoA Hydrolase Proteins 0.000 description 4
- 241000777575 Pyrobaculum aerophilum str. IM2 Species 0.000 description 4
- 241001061294 Pyrobaculum calidifontis Species 0.000 description 4
- 241000432808 Pyrococcus abyssi GE5 Species 0.000 description 4
- 241000836453 Pyrococcus furiosus COM1 Species 0.000 description 4
- 241000522615 Pyrococcus horikoshii Species 0.000 description 4
- 108010053763 Pyruvate Carboxylase Proteins 0.000 description 4
- 102100039895 Pyruvate carboxylase, mitochondrial Human genes 0.000 description 4
- 101710182361 Pyruvate:ferredoxin oxidoreductase Proteins 0.000 description 4
- 241001659987 Staphylothermus hellenicus Species 0.000 description 4
- 102000019259 Succinate Dehydrogenase Human genes 0.000 description 4
- 108010012901 Succinate Dehydrogenase Proteins 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
- 241000696041 Thermococcus onnurineus Species 0.000 description 4
- 241000205174 Thermofilum Species 0.000 description 4
- 241001491085 Thermofilum pendens Hrk 5 Species 0.000 description 4
- 241000343917 Thermoproteus tenax Kra 1 Species 0.000 description 4
- 101710137710 Thioesterase 1/protease 1/lysophospholipase L1 Proteins 0.000 description 4
- 238000009825 accumulation Methods 0.000 description 4
- 229960000583 acetic acid Drugs 0.000 description 4
- SPNAEHGLBRRCGL-BIEWRJSYSA-N adipoyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CCCCC(O)=O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 SPNAEHGLBRRCGL-BIEWRJSYSA-N 0.000 description 4
- 150000001336 alkenes Chemical class 0.000 description 4
- 230000004186 co-expression Effects 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000007865 diluting Methods 0.000 description 4
- 230000004907 flux Effects 0.000 description 4
- 238000012239 gene modification Methods 0.000 description 4
- 230000005017 genetic modification Effects 0.000 description 4
- 235000013617 genetically modified food Nutrition 0.000 description 4
- 230000006698 induction Effects 0.000 description 4
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 150000002576 ketones Chemical class 0.000 description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000006166 lysate Substances 0.000 description 4
- TWXDDNPPQUTEOV-FVGYRXGTSA-N methamphetamine hydrochloride Chemical compound Cl.CN[C@@H](C)CC1=CC=CC=C1 TWXDDNPPQUTEOV-FVGYRXGTSA-N 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 4
- 239000010452 phosphate Substances 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000006467 substitution reaction Methods 0.000 description 4
- 239000001117 sulphuric acid Substances 0.000 description 4
- 235000011149 sulphuric acid Nutrition 0.000 description 4
- 230000008685 targeting Effects 0.000 description 4
- OFVLGDICTFRJMM-WESIUVDSSA-N tetracycline Chemical compound C1=CC=C2[C@](O)(C)[C@H]3C[C@H]4[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O OFVLGDICTFRJMM-WESIUVDSSA-N 0.000 description 4
- 238000005820 transferase reaction Methods 0.000 description 4
- 238000013519 translation Methods 0.000 description 4
- GUAHPAJOXVYFON-ZETCQYMHSA-N (8S)-8-amino-7-oxononanoic acid zwitterion Chemical compound C[C@H](N)C(=O)CCCCCC(O)=O GUAHPAJOXVYFON-ZETCQYMHSA-N 0.000 description 3
- 108010063585 (R)-citramalate synthase Proteins 0.000 description 3
- ZFXICKRXPZTFPB-FZHFFJAKSA-N (Z)-2,3-dehydroadipoyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)\C=C/CCC(O)=O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZFXICKRXPZTFPB-FZHFFJAKSA-N 0.000 description 3
- HNSDLXPSAYFUHK-UHFFFAOYSA-N 1,4-bis(2-ethylhexyl) sulfosuccinate Chemical compound CCCCC(CC)COC(=O)CC(S(O)(=O)=O)C(=O)OCC(CC)CCCC HNSDLXPSAYFUHK-UHFFFAOYSA-N 0.000 description 3
- OTEACGAEDCIMBS-FOLKQPSDSA-N 3-hydroxyadipyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC(O)CCC(O)=O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 OTEACGAEDCIMBS-FOLKQPSDSA-N 0.000 description 3
- 108030005924 3-hydroxypropionyl-CoA dehydratases Proteins 0.000 description 3
- 108010039636 3-isopropylmalate dehydrogenase Proteins 0.000 description 3
- JVQYSWDUAOAHFM-UHFFFAOYSA-N 3-methyl-2-oxovaleric acid Chemical compound CCC(C)C(=O)C(O)=O JVQYSWDUAOAHFM-UHFFFAOYSA-N 0.000 description 3
- VKKKAAPGXHWXOO-BIEWRJSYSA-N 3-oxoadipyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC(=O)CCC(O)=O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 VKKKAAPGXHWXOO-BIEWRJSYSA-N 0.000 description 3
- 108010006229 Acetyl-CoA C-acetyltransferase Proteins 0.000 description 3
- 108700001448 Aldo-keto reductase family 1 member A1 Proteins 0.000 description 3
- 241000848220 Aquincola tertiaricarbonis Species 0.000 description 3
- 101100389345 Bacillus subtilis (strain 168) ndoA gene Proteins 0.000 description 3
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 101100366937 Caenorhabditis elegans sto-4 gene Proteins 0.000 description 3
- 241001112696 Clostridia Species 0.000 description 3
- 241000186542 Clostridium baratii Species 0.000 description 3
- 241000949098 Coprococcus comes Species 0.000 description 3
- 108700016168 Dihydroxy-acid dehydratases Proteins 0.000 description 3
- 102000057412 Diphosphomevalonate decarboxylases Human genes 0.000 description 3
- 108700035271 EC 1.1.1.2 Proteins 0.000 description 3
- 241001379910 Ephemera danica Species 0.000 description 3
- 108010087894 Fatty acid desaturases Proteins 0.000 description 3
- 102000009114 Fatty acid desaturases Human genes 0.000 description 3
- 108090000331 Firefly luciferases Proteins 0.000 description 3
- 102000003983 Flavoproteins Human genes 0.000 description 3
- 108010057573 Flavoproteins Proteins 0.000 description 3
- 108010036781 Fumarate Hydratase Proteins 0.000 description 3
- 102100036160 Fumarate hydratase, mitochondrial Human genes 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 241001131718 Haloarcula hispanica ATCC 33960 Species 0.000 description 3
- 241000756428 Haloarcula hispanica N601 Species 0.000 description 3
- 241000981400 Haloferax volcanii DS2 Species 0.000 description 3
- 241000868218 Halogeometricum borinquense Species 0.000 description 3
- 241000204930 Halorubrum lacusprofundi Species 0.000 description 3
- 108010020056 Hydrogenase Proteins 0.000 description 3
- 102000002284 Hydroxymethylglutaryl-CoA Synthase Human genes 0.000 description 3
- 241001139408 Methanosarcina acetivorans C2A Species 0.000 description 3
- 241000205274 Methanosarcina mazei Species 0.000 description 3
- 241000403956 Methanosarcina mazei Tuc01 Species 0.000 description 3
- ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 description 3
- 241001417623 Natrinema pellirubrum DSM 15624 Species 0.000 description 3
- 241000121076 Natronomonas moolapensis 8.8.11 Species 0.000 description 3
- 239000002202 Polyethylene glycol Substances 0.000 description 3
- 241000514899 Pyrobaculum islandicum DSM 4184 Species 0.000 description 3
- 241000439254 Pyrobaculum neutrophilum V24Sta Species 0.000 description 3
- 241000881945 Pyrobaculum sp. Species 0.000 description 3
- 241001115170 Pyrococcus furiosus DSM 3638 Species 0.000 description 3
- 241001120653 Pyrococcus yayanosii CH1 Species 0.000 description 3
- 241000531138 Pyrolobus fumarii Species 0.000 description 3
- 108010031852 Pyruvate Synthase Proteins 0.000 description 3
- 241000589625 Ralstonia pickettii Species 0.000 description 3
- 102000011929 Succinate-CoA Ligases Human genes 0.000 description 3
- 108010075728 Succinate-CoA Ligases Proteins 0.000 description 3
- 241001621851 Thermanaerovibrio Species 0.000 description 3
- 241000847591 Thermococcus barophilus MP Species 0.000 description 3
- 241000357036 Thermococcus cleftensis Species 0.000 description 3
- 241001054916 Thermococcus litoralis DSM 5473 Species 0.000 description 3
- 241001135697 Thermodesulfovibrio yellowstonii Species 0.000 description 3
- 241000870821 Thermoproteus uzoniensis 768-20 Species 0.000 description 3
- 241000589892 Treponema denticola Species 0.000 description 3
- 108010084631 acetolactate decarboxylase Proteins 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 108010011384 acyl-CoA dehydrogenase (NADP+) Proteins 0.000 description 3
- 229940024606 amino acid Drugs 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000027455 binding Effects 0.000 description 3
- PASOAYSIZAJOCT-UHFFFAOYSA-N butanoic acid Chemical compound CCCC(O)=O.CCCC(O)=O PASOAYSIZAJOCT-UHFFFAOYSA-N 0.000 description 3
- MLUCVPSAIODCQM-NSCUHMNNSA-N crotonaldehyde Chemical compound C\C=C\C=O MLUCVPSAIODCQM-NSCUHMNNSA-N 0.000 description 3
- MLUCVPSAIODCQM-UHFFFAOYSA-N crotonaldehyde Natural products CC=CC=O MLUCVPSAIODCQM-UHFFFAOYSA-N 0.000 description 3
- LDHQCZJRKDOVOX-NSCUHMNNSA-N crotonic acid Chemical compound C\C=C\C(O)=O LDHQCZJRKDOVOX-NSCUHMNNSA-N 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 229920001971 elastomer Polymers 0.000 description 3
- 239000013613 expression plasmid Substances 0.000 description 3
- 238000010353 genetic engineering Methods 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 239000000833 heterodimer Substances 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 230000002779 inactivation Effects 0.000 description 3
- 239000000411 inducer Substances 0.000 description 3
- 230000001939 inductive effect Effects 0.000 description 3
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 3
- UYVZIWWBJMYRCD-ZMHDXICWSA-N isovaleryl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC(C)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 UYVZIWWBJMYRCD-ZMHDXICWSA-N 0.000 description 3
- 239000007791 liquid phase Substances 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 101150048352 mazF gene Proteins 0.000 description 3
- 230000002503 metabolic effect Effects 0.000 description 3
- 239000000178 monomer Substances 0.000 description 3
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 3
- 230000036961 partial effect Effects 0.000 description 3
- 229920001223 polyethylene glycol Polymers 0.000 description 3
- 238000003752 polymerase chain reaction Methods 0.000 description 3
- 238000004064 recycling Methods 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 108091008146 restriction endonucleases Proteins 0.000 description 3
- 239000005060 rubber Substances 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 230000002269 spontaneous effect Effects 0.000 description 3
- VNOYUJKHFWYWIR-ITIYDSSPSA-N succinyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CCC(O)=O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 VNOYUJKHFWYWIR-ITIYDSSPSA-N 0.000 description 3
- 150000008163 sugars Chemical class 0.000 description 3
- OTVAEFIXJLOWRX-NXEZZACHSA-N thiamphenicol Chemical compound CS(=O)(=O)C1=CC=C([C@@H](O)[C@@H](CO)NC(=O)C(Cl)Cl)C=C1 OTVAEFIXJLOWRX-NXEZZACHSA-N 0.000 description 3
- 229960003053 thiamphenicol Drugs 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- LYNVNYDEQMMNMZ-XGXNYEOVSA-N 2-methylbutanoyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C(C)CC)O[C@H]1N1C2=NC=NC(N)=C2N=C1 LYNVNYDEQMMNMZ-XGXNYEOVSA-N 0.000 description 2
- MWMOPIVLTLEUJO-UHFFFAOYSA-N 2-oxopropanoic acid;phosphoric acid Chemical compound OP(O)(O)=O.CC(=O)C(O)=O MWMOPIVLTLEUJO-UHFFFAOYSA-N 0.000 description 2
- HPMGFDVTYHWBAG-UHFFFAOYSA-N 3-hydroxyhexanoic acid Chemical compound CCCC(O)CC(O)=O HPMGFDVTYHWBAG-UHFFFAOYSA-N 0.000 description 2
- ALRHLSYJTWAHJZ-UHFFFAOYSA-M 3-hydroxypropionate Chemical compound OCCC([O-])=O ALRHLSYJTWAHJZ-UHFFFAOYSA-M 0.000 description 2
- 108010028984 3-isopropylmalate dehydratase Proteins 0.000 description 2
- 102100026105 3-ketoacyl-CoA thiolase, mitochondrial Human genes 0.000 description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
- YCPXWRQRBFJBPZ-UHFFFAOYSA-N 5-sulfosalicylic acid Chemical compound OC(=O)C1=CC(S(O)(=O)=O)=CC=C1O YCPXWRQRBFJBPZ-UHFFFAOYSA-N 0.000 description 2
- 230000002407 ATP formation Effects 0.000 description 2
- 102000005345 Acetyl-CoA C-acetyltransferase Human genes 0.000 description 2
- 241000607516 Aeromonas caviae Species 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 241001657391 Archaeoglobus profundus Species 0.000 description 2
- 241000006176 Archaeoglobus veneficus SNP6 Species 0.000 description 2
- 101150056981 BID gene Proteins 0.000 description 2
- 101000782236 Bothrops leucurus Thrombin-like enzyme leucurobin Proteins 0.000 description 2
- 241000193174 Butyrivibrio crossotus Species 0.000 description 2
- 102100031668 Chromodomain Y-like protein Human genes 0.000 description 2
- 101710168556 Chromodomain Y-like protein Proteins 0.000 description 2
- 241000070918 Cima Species 0.000 description 2
- 241001522206 Clostridium algidicarnis Species 0.000 description 2
- 241001147768 Clostridium argentinense Species 0.000 description 2
- 241001611022 Clostridium carboxidivorans Species 0.000 description 2
- 241001509496 Clostridium celatum Species 0.000 description 2
- 241000206044 Clostridium chauvoei Species 0.000 description 2
- 241000788977 Clostridium colicanis Species 0.000 description 2
- 241000272479 Clostridium diolis Species 0.000 description 2
- 241000328950 Clostridium drakei Species 0.000 description 2
- 241000779784 Clostridium hydrogeniformans Species 0.000 description 2
- 241001509477 Clostridium intestinale Species 0.000 description 2
- 241001147791 Clostridium paraputrificum Species 0.000 description 2
- 241000429427 Clostridium saccharobutylicum Species 0.000 description 2
- 241000186587 Clostridium scatologenes Species 0.000 description 2
- 241000936120 Clostridium senegalense Species 0.000 description 2
- 241001318996 Clostridium sulfidigenes Species 0.000 description 2
- 241000186520 Clostridium tetanomorphum Species 0.000 description 2
- 101710188748 Crotonyl-CoA hydratase Proteins 0.000 description 2
- 238000001712 DNA sequencing Methods 0.000 description 2
- 241000605762 Desulfovibrio vulgaris Species 0.000 description 2
- 101100070376 Dictyostelium discoideum alad gene Proteins 0.000 description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 2
- 108700034992 EC 1.1.1.80 Proteins 0.000 description 2
- 241000672609 Escherichia coli BL21 Species 0.000 description 2
- 241001198387 Escherichia coli BL21(DE3) Species 0.000 description 2
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 description 2
- 229930091371 Fructose Natural products 0.000 description 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 2
- 239000005715 Fructose Substances 0.000 description 2
- 241001003011 Geobacter sulfurreducens PCA Species 0.000 description 2
- 241000205063 Haloarcula marismortui Species 0.000 description 2
- 241000204988 Haloferax mediterranei Species 0.000 description 2
- 101000958922 Homo sapiens Diphosphomevalonate decarboxylase Proteins 0.000 description 2
- 241000773735 Ignicoccus hospitalis KIN4/I Species 0.000 description 2
- RRHGJUQNOFWUDK-UHFFFAOYSA-N Isoprene Chemical compound CC(=C)C=C RRHGJUQNOFWUDK-UHFFFAOYSA-N 0.000 description 2
- 108010000200 Ketol-acid reductoisomerase Proteins 0.000 description 2
- 241001468178 Kyrpidia tusciae Species 0.000 description 2
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- 108010026217 Malate Dehydrogenase Proteins 0.000 description 2
- 102000013460 Malate Dehydrogenase Human genes 0.000 description 2
- 241000203407 Methanocaldococcus jannaschii Species 0.000 description 2
- 241001042243 Methanocella arvoryzae MRE50 Species 0.000 description 2
- 241001276824 Methanocella conradii HZ254 Species 0.000 description 2
- 241000921850 Methanocella paludicola SANAE Species 0.000 description 2
- 241000204637 Methanohalobium evestigatum Species 0.000 description 2
- ROAIXOJGRFKICW-UHFFFAOYSA-N Methenamine hippurate Chemical compound C1N(C2)CN3CN1CN2C3.OC(=O)CNC(=O)C1=CC=CC=C1 ROAIXOJGRFKICW-UHFFFAOYSA-N 0.000 description 2
- 102100026552 Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial Human genes 0.000 description 2
- 101100340782 Oryza sativa subsp. indica ILI1 gene Proteins 0.000 description 2
- 101100340783 Oryza sativa subsp. japonica BHLH154 gene Proteins 0.000 description 2
- 101000958925 Panax ginseng Diphosphomevalonate decarboxylase 1 Proteins 0.000 description 2
- 108091000080 Phosphotransferase Proteins 0.000 description 2
- 229920000331 Polyhydroxybutyrate Polymers 0.000 description 2
- 101710104378 Putative malate oxidoreductase [NAD] Proteins 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- 241000205156 Pyrococcus furiosus Species 0.000 description 2
- 241000191043 Rhodobacter sphaeroides Species 0.000 description 2
- 241000711837 Roseburia sp. Species 0.000 description 2
- 108030005950 Short-chain-enoyl-CoA hydratases Proteins 0.000 description 2
- 101000581497 Solanum tuberosum 2-methylacyl-CoA dehydrogenase, mitochondrial Proteins 0.000 description 2
- 241000543642 Sporomusa silvacetica Species 0.000 description 2
- 241000205077 Staphylothermus marinus Species 0.000 description 2
- 241000145545 Streptomyces collinus Species 0.000 description 2
- 239000004098 Tetracycline Substances 0.000 description 2
- 241000295829 Thermococcus gammatolerans EJ3 Species 0.000 description 2
- 241000981880 Thermococcus kodakarensis KOD1 Species 0.000 description 2
- 241000204673 Thermoplasma acidophilum Species 0.000 description 2
- 241000435340 Thermoplasma volcanium GSS1 Species 0.000 description 2
- 241000531145 Thermosphaera aggregans Species 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 108091000039 acetoacetyl-CoA reductase Proteins 0.000 description 2
- 238000013019 agitation Methods 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 238000005415 bioluminescence Methods 0.000 description 2
- 230000029918 bioluminescence Effects 0.000 description 2
- 150000001735 carboxylic acids Chemical class 0.000 description 2
- 239000012159 carrier gas Substances 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 239000013592 cell lysate Substances 0.000 description 2
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 2
- 229960005091 chloramphenicol Drugs 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 239000003245 coal Substances 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- RWGFKTVRMDUZSP-UHFFFAOYSA-N cumene Chemical compound CC(C)C1=CC=CC=C1 RWGFKTVRMDUZSP-UHFFFAOYSA-N 0.000 description 2
- 238000010543 cumene process Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 229910001882 dioxygen Inorganic materials 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- 238000004134 energy conservation Methods 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 238000001976 enzyme digestion Methods 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 239000013604 expression vector Substances 0.000 description 2
- 238000012262 fermentative production Methods 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000000446 fuel Substances 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 238000003209 gene knockout Methods 0.000 description 2
- 108010087331 glutaconate CoA-transferase Proteins 0.000 description 2
- 229940093915 gynecological organic acid Drugs 0.000 description 2
- 239000001307 helium Substances 0.000 description 2
- 229910052734 helium Inorganic materials 0.000 description 2
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 2
- 101150055960 hemB gene Proteins 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 108010072869 indolepyruvate decarboxylase Proteins 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 229960000310 isoleucine Drugs 0.000 description 2
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 235000014655 lactic acid Nutrition 0.000 description 2
- WQVJUBFKFCDYDQ-BBWFWOEESA-N leubethanol Natural products C1=C(C)C=C2[C@H]([C@H](CCC=C(C)C)C)CC[C@@H](C)C2=C1O WQVJUBFKFCDYDQ-BBWFWOEESA-N 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 238000004811 liquid chromatography Methods 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- 238000012269 metabolic engineering Methods 0.000 description 2
- 108010071806 methylcrotonoyl-CoA carboxylase Proteins 0.000 description 2
- 238000002552 multiple reaction monitoring Methods 0.000 description 2
- SYSQUGFVNFXIIT-UHFFFAOYSA-N n-[4-(1,3-benzoxazol-2-yl)phenyl]-4-nitrobenzenesulfonamide Chemical class C1=CC([N+](=O)[O-])=CC=C1S(=O)(=O)NC1=CC=C(C=2OC3=CC=CC=C3N=2)C=C1 SYSQUGFVNFXIIT-UHFFFAOYSA-N 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 235000005985 organic acids Nutrition 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 239000012466 permeate Substances 0.000 description 2
- 102000020233 phosphotransferase Human genes 0.000 description 2
- 239000005015 poly(hydroxybutyrate) Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 239000008057 potassium phosphate buffer Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- VAAHKRMGOFIORX-SNIDVWGTSA-N s-[2-[3-[[(2r)-4-[[[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-4-hydroxy-3-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl]oxy-2-hydroxy-3,3-dimethylbutanoyl]amino]propanoylamino]ethyl] 3-hydroxyhexanethioate Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC(O)CCC)O[C@H]1N1C2=NC=NC(N)=C2N=C1 VAAHKRMGOFIORX-SNIDVWGTSA-N 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 2
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 2
- 229960000268 spectinomycin Drugs 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 230000000707 stereoselective effect Effects 0.000 description 2
- 101150026728 tesB gene Proteins 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 229960002180 tetracycline Drugs 0.000 description 2
- 235000019364 tetracycline Nutrition 0.000 description 2
- 150000003522 tetracyclines Chemical class 0.000 description 2
- IEDVJHCEMCRBQM-UHFFFAOYSA-N trimethoprim Chemical compound COC1=C(OC)C(OC)=CC(CC=2C(=NC(N)=NC=2)N)=C1 IEDVJHCEMCRBQM-UHFFFAOYSA-N 0.000 description 2
- 229960001082 trimethoprim Drugs 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 230000004572 zinc-binding Effects 0.000 description 2
- AOFUBOWZWQFQJU-SNOJBQEQSA-N (2r,3s,4s,5r)-2,5-bis(hydroxymethyl)oxolane-2,3,4-triol;(2s,3r,4s,5s,6r)-6-(hydroxymethyl)oxane-2,3,4,5-tetrol Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O.OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@@H]1O AOFUBOWZWQFQJU-SNOJBQEQSA-N 0.000 description 1
- CABVTRNMFUVUDM-VRHQGPGLSA-N (3S)-3-hydroxy-3-methylglutaryl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C[C@@](O)(CC(O)=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 CABVTRNMFUVUDM-VRHQGPGLSA-N 0.000 description 1
- NEJDKFPXHQRVMV-UHFFFAOYSA-N (E)-2-Methyl-2-buten-1-ol Natural products CC=C(C)CO NEJDKFPXHQRVMV-UHFFFAOYSA-N 0.000 description 1
- DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 description 1
- AFENDNXGAFYKQO-GSVOUGTGSA-N (R)-2-hydroxybutyric acid Chemical compound CC[C@@H](O)C(O)=O AFENDNXGAFYKQO-GSVOUGTGSA-N 0.000 description 1
- 108010062721 (R)-2-methylmalate dehydratase Proteins 0.000 description 1
- WHBMMWSBFZVSSR-GSVOUGTGSA-N (R)-3-hydroxybutyric acid Chemical compound C[C@@H](O)CC(O)=O WHBMMWSBFZVSSR-GSVOUGTGSA-N 0.000 description 1
- PUPZLCDOIYMWBV-SCSAIBSYSA-N (R)-butane-1,3-diol Chemical compound C[C@@H](O)CCO PUPZLCDOIYMWBV-SCSAIBSYSA-N 0.000 description 1
- SYTBZMRGLBWNTM-SNVBAGLBSA-N (R)-flurbiprofen Chemical compound FC1=CC([C@H](C(O)=O)C)=CC=C1C1=CC=CC=C1 SYTBZMRGLBWNTM-SNVBAGLBSA-N 0.000 description 1
- PUPZLCDOIYMWBV-BYPYZUCNSA-N (S)-butane-1,3-diol Chemical compound C[C@H](O)CCO PUPZLCDOIYMWBV-BYPYZUCNSA-N 0.000 description 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- VXNZUUAINFGPBY-UHFFFAOYSA-N 1-Butene Chemical compound CCC=C VXNZUUAINFGPBY-UHFFFAOYSA-N 0.000 description 1
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- PKAUICCNAWQPAU-UHFFFAOYSA-N 2-(4-chloro-2-methylphenoxy)acetic acid;n-methylmethanamine Chemical compound CNC.CC1=CC(Cl)=CC=C1OCC(O)=O PKAUICCNAWQPAU-UHFFFAOYSA-N 0.000 description 1
- BTVWZWFKMIUSGS-UHFFFAOYSA-N 2-methylpropane-1,2-diol Chemical compound CC(C)(O)CO BTVWZWFKMIUSGS-UHFFFAOYSA-N 0.000 description 1
- XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 description 1
- XQMVBICWFFHDNN-UHFFFAOYSA-N 5-amino-4-chloro-2-phenylpyridazin-3-one;(2-ethoxy-3,3-dimethyl-2h-1-benzofuran-5-yl) methanesulfonate Chemical compound O=C1C(Cl)=C(N)C=NN1C1=CC=CC=C1.C1=C(OS(C)(=O)=O)C=C2C(C)(C)C(OCC)OC2=C1 XQMVBICWFFHDNN-UHFFFAOYSA-N 0.000 description 1
- HLXHCNWEVQNNKA-UHFFFAOYSA-N 5-methoxy-2,3-dihydro-1h-inden-2-amine Chemical compound COC1=CC=C2CC(N)CC2=C1 HLXHCNWEVQNNKA-UHFFFAOYSA-N 0.000 description 1
- 108030002957 Acetate CoA-transferases Proteins 0.000 description 1
- 241001468163 Acetobacterium woodii Species 0.000 description 1
- 108010003902 Acetyl-CoA C-acyltransferase Proteins 0.000 description 1
- 241000423335 Aeropyrum pernix K1 Species 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 108020005544 Antisense RNA Proteins 0.000 description 1
- 241000219195 Arabidopsis thaliana Species 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 208000035404 Autolysis Diseases 0.000 description 1
- 241000193738 Bacillus anthracis Species 0.000 description 1
- 241000194108 Bacillus licheniformis Species 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 1
- 238000009010 Bradford assay Methods 0.000 description 1
- 241000605902 Butyrivibrio Species 0.000 description 1
- MGONSHHJTCSVHI-UHFFFAOYSA-N C(CC(=O)C)(=O)OP(=O)(O)O Chemical compound C(CC(=O)C)(=O)OP(=O)(O)O MGONSHHJTCSVHI-UHFFFAOYSA-N 0.000 description 1
- 108091033409 CRISPR Proteins 0.000 description 1
- 238000010354 CRISPR gene editing Methods 0.000 description 1
- SCLQIEGBOYRIEH-GDZMCRPHSA-N C[C@@H](O)CC(O)=O.CC(C)(COP(O)(=O)OP(O)(=O)OC[C@H]1O[C@H]([C@H](O)[C@@H]1OP(O)(O)=O)n1cnc2c(N)ncnc12)[C@@H](O)C(=O)NCCC(=O)NCCS Chemical compound C[C@@H](O)CC(O)=O.CC(C)(COP(O)(=O)OP(O)(=O)OC[C@H]1O[C@H]([C@H](O)[C@@H]1OP(O)(O)=O)n1cnc2c(N)ncnc12)[C@@H](O)C(=O)NCCC(=O)NCCS SCLQIEGBOYRIEH-GDZMCRPHSA-N 0.000 description 1
- 101100369913 Caenorhabditis elegans tag-175 gene Proteins 0.000 description 1
- 241000716167 Caloramator australicus Species 0.000 description 1
- 241000062075 Caloramator sp. Species 0.000 description 1
- 241001395745 Candidatus Caldiarchaeum Species 0.000 description 1
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 1
- 206010057248 Cell death Diseases 0.000 description 1
- 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 1
- 108020004638 Circular DNA Proteins 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 101900188223 Clostridium acetobutylicum Phosphate butyryltransferase Proteins 0.000 description 1
- 241000306276 Clostridium akagii Species 0.000 description 1
- 241000719667 Clostridium arbusti Species 0.000 description 1
- 101100322572 Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) adc gene Proteins 0.000 description 1
- 241001335108 Clostridium bornimense Species 0.000 description 1
- 241001509415 Clostridium botulinum A Species 0.000 description 1
- 241000394482 Clostridium botulinum A str. Hall Species 0.000 description 1
- 241000193455 Clostridium cadaveris Species 0.000 description 1
- 241001331740 Clostridium lundense Species 0.000 description 1
- 241001468167 Clostridium magnum Species 0.000 description 1
- 241000193470 Clostridium sporogenes Species 0.000 description 1
- 241001464949 Coprococcus eutactus Species 0.000 description 1
- 102000018832 Cytochromes Human genes 0.000 description 1
- 108010052832 Cytochromes Proteins 0.000 description 1
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 1
- RGHNJXZEOKUKBD-SQOUGZDYSA-M D-gluconate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O RGHNJXZEOKUKBD-SQOUGZDYSA-M 0.000 description 1
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 1
- 230000007023 DNA restriction-modification system Effects 0.000 description 1
- 241000205229 Desulfurococcus mucosus Species 0.000 description 1
- 108700040484 Diphosphomevalonate decarboxylases Proteins 0.000 description 1
- 108700033402 EC 1.1.1.38 Proteins 0.000 description 1
- 108700033379 EC 1.1.1.40 Proteins 0.000 description 1
- 108700034993 EC 1.1.1.86 Proteins 0.000 description 1
- 108700034911 EC 3.1.2.- Proteins 0.000 description 1
- 101150014913 ERG13 gene Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102100021822 Enoyl-CoA hydratase, mitochondrial Human genes 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- 241001531192 Eubacterium ventriosum Species 0.000 description 1
- 241001531182 Eubacterium xylanophilum Species 0.000 description 1
- 241000195619 Euglena gracilis Species 0.000 description 1
- 241000228882 Fervidicella metallireducens Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 101000839020 Homo sapiens Hydroxymethylglutaryl-CoA synthase, mitochondrial Proteins 0.000 description 1
- 101000581533 Homo sapiens Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial Proteins 0.000 description 1
- 101001056160 Homo sapiens Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial Proteins 0.000 description 1
- 108010063370 Homoaconitate hydratase Proteins 0.000 description 1
- 241001603562 Ignisphaera aggregans DSM 17230 Species 0.000 description 1
- NHTMVDHEPJAVLT-UHFFFAOYSA-N Isooctane Chemical compound CC(C)CC(C)(C)C NHTMVDHEPJAVLT-UHFFFAOYSA-N 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 1
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- 239000006142 Luria-Bertani Agar Substances 0.000 description 1
- OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 1
- 241000157876 Metallosphaera sedula Species 0.000 description 1
- 101100395468 Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2) Msed_2001 gene Proteins 0.000 description 1
- CERQOIWHTDAKMF-UHFFFAOYSA-N Methacrylic acid Chemical compound CC(=C)C(O)=O CERQOIWHTDAKMF-UHFFFAOYSA-N 0.000 description 1
- 241000947423 Methanocorpusculum labreanum Z Species 0.000 description 1
- 241001491087 Methanoculleus marisnigri JR1 Species 0.000 description 1
- 241001484825 Methanolobus psychrophilus R15 Species 0.000 description 1
- 241001450793 Methanomethylovorans hollandica Species 0.000 description 1
- 241001592722 Methanosaeta harundinacea 6Ac Species 0.000 description 1
- 241001013737 Methanosalsum zhilinae DSM 4017 Species 0.000 description 1
- 241000134675 Methanosarcina barkeri str. Fusaro Species 0.000 description 1
- 241001538098 Methanosphaerula palustris Species 0.000 description 1
- 241001649418 Methanospirillum hungatei JF-1 Species 0.000 description 1
- 241001302042 Methanothermobacter thermautotrophicus Species 0.000 description 1
- 241000767818 Methanothrix thermoacetophila PT Species 0.000 description 1
- 102100027320 Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial Human genes 0.000 description 1
- 241001533203 Methylomicrobium Species 0.000 description 1
- 108010006519 Molecular Chaperones Proteins 0.000 description 1
- 241000193459 Moorella thermoacetica Species 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 description 1
- XCOBLONWWXQEBS-KPKJPENVSA-N N,O-bis(trimethylsilyl)trifluoroacetamide Chemical compound C[Si](C)(C)O\C(C(F)(F)F)=N\[Si](C)(C)C XCOBLONWWXQEBS-KPKJPENVSA-N 0.000 description 1
- 102100032457 NAD-dependent malic enzyme, mitochondrial Human genes 0.000 description 1
- 102100023175 NADP-dependent malic enzyme Human genes 0.000 description 1
- 241000975077 Natrinema sp. Species 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 229920002302 Nylon 6,6 Polymers 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241001509483 Oxobacter pfennigii Species 0.000 description 1
- 238000009004 PCR Kit Methods 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 241000577082 Peptostreptococcaceae bacterium Species 0.000 description 1
- 102100027506 Peroxisomal trans-2-enoyl-CoA reductase Human genes 0.000 description 1
- 102000009097 Phosphorylases Human genes 0.000 description 1
- 108010073135 Phosphorylases Proteins 0.000 description 1
- 241001632455 Picrophilus torridus Species 0.000 description 1
- 229920001273 Polyhydroxy acid Polymers 0.000 description 1
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- 108020005091 Replication Origin Proteins 0.000 description 1
- 241000190984 Rhodospirillum rubrum Species 0.000 description 1
- 229910006722 SNO3 Inorganic materials 0.000 description 1
- 101100042909 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) SNO2 gene Proteins 0.000 description 1
- 101100042910 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) SNO3 gene Proteins 0.000 description 1
- 206010039792 Seborrhoea Diseases 0.000 description 1
- 241000270295 Serpentes Species 0.000 description 1
- 108020004459 Small interfering RNA Proteins 0.000 description 1
- XOLBLPGZBRYERU-UHFFFAOYSA-N SnO2 Inorganic materials O=[Sn]=O XOLBLPGZBRYERU-UHFFFAOYSA-N 0.000 description 1
- 241000204376 Sporomusa ovata Species 0.000 description 1
- 241000217849 Sporomusa sphaeroides Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 241001468227 Streptomyces avermitilis Species 0.000 description 1
- 241000187432 Streptomyces coelicolor Species 0.000 description 1
- 241001580973 Subdoligranulum variabile Species 0.000 description 1
- 102100035726 Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial Human genes 0.000 description 1
- 241000202694 Thermoanaerobacter wiegelii Species 0.000 description 1
- 241000520824 Thermobrachium celere Species 0.000 description 1
- 241000847424 Thermogladius calderae 1633 Species 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- 241000499912 Trichoderma reesei Species 0.000 description 1
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 1
- 108020002494 acetyltransferase Proteins 0.000 description 1
- 102000005421 acetyltransferase Human genes 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- BTGRAWJCKBQKAO-UHFFFAOYSA-N adiponitrile Chemical compound N#CCCCCC#N BTGRAWJCKBQKAO-UHFFFAOYSA-N 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000004099 anaerobic respiration Effects 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 210000004507 artificial chromosome Anatomy 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 229940065181 bacillus anthracis Drugs 0.000 description 1
- 101150052778 bdh gene Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001277 beta hydroxy acids Chemical class 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 229960003237 betaine Drugs 0.000 description 1
- 229920000704 biodegradable plastic Polymers 0.000 description 1
- 229920002988 biodegradable polymer Polymers 0.000 description 1
- 239000004621 biodegradable polymer Substances 0.000 description 1
- 239000002551 biofuel Substances 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000006696 biosynthetic metabolic pathway Effects 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- UVMPXOYNLLXNTR-UHFFFAOYSA-N butan-1-ol;ethanol;propan-2-one Chemical compound CCO.CC(C)=O.CCCCO UVMPXOYNLLXNTR-UHFFFAOYSA-N 0.000 description 1
- RFAZFSACZIVZDV-UHFFFAOYSA-N butan-2-one Chemical compound CCC(C)=O.CCC(C)=O RFAZFSACZIVZDV-UHFFFAOYSA-N 0.000 description 1
- UPLCYFWQZHLPFH-UHFFFAOYSA-N butane-1,1-diol;butane-2,3-diol Chemical compound CCCC(O)O.CC(O)C(C)O UPLCYFWQZHLPFH-UHFFFAOYSA-N 0.000 description 1
- IAQRGUVFOMOMEM-UHFFFAOYSA-N butene Natural products CC=CC IAQRGUVFOMOMEM-UHFFFAOYSA-N 0.000 description 1
- 238000004422 calculation algorithm Methods 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000006229 carbon black Substances 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 230000006315 carbonylation Effects 0.000 description 1
- 238000005810 carbonylation reaction Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000001805 chlorine compounds Chemical class 0.000 description 1
- 238000013375 chromatographic separation Methods 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000000571 coke Substances 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 239000003184 complementary RNA Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000008406 cosmetic ingredient Substances 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- WCASXYBKJHWFMY-UHFFFAOYSA-N crotyl alcohol Chemical compound CC=CCO WCASXYBKJHWFMY-UHFFFAOYSA-N 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- JVSWJIKNEAIKJW-UHFFFAOYSA-N dimethyl-hexane Natural products CCCCCC(C)C JVSWJIKNEAIKJW-UHFFFAOYSA-N 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000009510 drug design Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 150000002085 enols Chemical class 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012847 fine chemical Substances 0.000 description 1
- LIYGYAHYXQDGEP-UHFFFAOYSA-N firefly oxyluciferin Natural products Oc1csc(n1)-c1nc2ccc(O)cc2s1 LIYGYAHYXQDGEP-UHFFFAOYSA-N 0.000 description 1
- 235000013355 food flavoring agent Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000004508 fractional distillation Methods 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 239000002816 fuel additive Substances 0.000 description 1
- 239000003517 fume Substances 0.000 description 1
- 239000003502 gasoline Substances 0.000 description 1
- 108091008053 gene clusters Proteins 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 229940050410 gluconate Drugs 0.000 description 1
- 230000034659 glycolysis Effects 0.000 description 1
- 238000003988 headspace gas chromatography Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- QKGYJVXSKCDGOK-UHFFFAOYSA-N hexane;propan-2-ol Chemical compound CC(C)O.CCCCCC QKGYJVXSKCDGOK-UHFFFAOYSA-N 0.000 description 1
- FUZZWVXGSFPDMH-UHFFFAOYSA-M hexanoate Chemical compound CCCCCC([O-])=O FUZZWVXGSFPDMH-UHFFFAOYSA-M 0.000 description 1
- 238000012203 high throughput assay Methods 0.000 description 1
- 238000013537 high throughput screening Methods 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 210000001822 immobilized cell Anatomy 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000004434 industrial solvent Substances 0.000 description 1
- 239000002440 industrial waste Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000000976 ink Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 101150066555 lacZ gene Proteins 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 239000012978 lignocellulosic material Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 238000000622 liquid--liquid extraction Methods 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 239000012139 lysis buffer Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000006241 metabolic reaction Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000001450 methanotrophic effect Effects 0.000 description 1
- 150000004702 methyl esters Chemical class 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000001823 molecular biology technique Methods 0.000 description 1
- DNIAPMSPPWPWGF-UHFFFAOYSA-N monopropylene glycol Natural products CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 1
- 239000010813 municipal solid waste Substances 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 239000002114 nanocomposite Substances 0.000 description 1
- 239000003345 natural gas Substances 0.000 description 1
- 239000006199 nebulizer Substances 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000037312 oily skin Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- JJVOROULKOMTKG-UHFFFAOYSA-N oxidized Photinus luciferin Chemical compound S1C2=CC(O)=CC=C2N=C1C1=NC(=O)CS1 JJVOROULKOMTKG-UHFFFAOYSA-N 0.000 description 1
- 125000004430 oxygen atom Chemical group O* 0.000 description 1
- MNBKLUUYKPBKDU-BBECNAHFSA-N palmitoyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CCCCCCCCCCCCCCC)O[C@H]1N1C2=NC=NC(N)=C2N=C1 MNBKLUUYKPBKDU-BBECNAHFSA-N 0.000 description 1
- 238000005373 pervaporation Methods 0.000 description 1
- 238000005504 petroleum refining Methods 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920001225 polyester resin Polymers 0.000 description 1
- 239000004645 polyester resin Substances 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 229920005749 polyurethane resin Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 238000000079 presaturation Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Natural products CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 description 1
- 235000013772 propylene glycol Nutrition 0.000 description 1
- 125000004805 propylene group Chemical group [H]C([H])([H])C([H])([*:1])C([H])([H])[*:2] 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 238000002708 random mutagenesis Methods 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000002407 reforming Methods 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 238000013515 script Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 230000028043 self proteolysis Effects 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 239000013605 shuttle vector Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 235000010265 sodium sulphite Nutrition 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 238000002470 solid-phase micro-extraction Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 108090000308 trans-2-enoyl-CoA reductase (NADPH) Proteins 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- IMFACGCPASFAPR-UHFFFAOYSA-N tributylamine Chemical compound CCCCN(CCCC)CCCC IMFACGCPASFAPR-UHFFFAOYSA-N 0.000 description 1
- 238000002525 ultrasonication Methods 0.000 description 1
- 238000000825 ultraviolet detection Methods 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 239000002912 waste gas Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000010925 yard waste Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/42—Hydroxy-carboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1217—Phosphotransferases with a carboxyl group as acceptor (2.7.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
- C12P7/26—Ketones
- C12P7/28—Acetone-containing products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
- C12P7/625—Polyesters of hydroxy carboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01002—Alcohol dehydrogenase (NADP+) (1.1.1.2), i.e. aldehyde reductase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
- C12Y102/07—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with an iron-sulfur protein as acceptor (1.2.7)
- C12Y102/07005—Aldehyde ferredoxin oxidoreductase (1.2.7.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01008—Phosphate acetyltransferase (2.3.1.8)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01009—Acetyl-CoA C-acetyltransferase (2.3.1.9)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01019—Phosphate butyryltransferase (2.3.1.19)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/02—Phosphotransferases with a carboxy group as acceptor (2.7.2)
- C12Y207/02001—Acetate kinase (2.7.2.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/02—Phosphotransferases with a carboxy group as acceptor (2.7.2)
- C12Y207/02007—Butyrate kinase (2.7.2.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/02—Thioester hydrolases (3.1.2)
- C12Y301/0202—Acyl-CoA hydrolase (3.1.2.20)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201562240850P | 2015-10-13 | 2015-10-13 | |
US62/240,850 | 2015-10-13 | ||
CA3051235A CA3051235C (en) | 2015-10-13 | 2016-10-13 | Genetically engineered bacterium comprising energy-generating fermentation pathway |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA3051235A Division CA3051235C (en) | 2015-10-13 | 2016-10-13 | Genetically engineered bacterium comprising energy-generating fermentation pathway |
Publications (2)
Publication Number | Publication Date |
---|---|
CA3151149A1 CA3151149A1 (en) | 2017-04-20 |
CA3151149C true CA3151149C (en) | 2024-03-26 |
Family
ID=58499646
Family Applications (6)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA3051234A Active CA3051234C (en) | 2015-10-13 | 2016-10-13 | Genetically engineered bacterium comprising energy-generating fermentation pathway |
CA3001596A Active CA3001596C (en) | 2015-10-13 | 2016-10-13 | Genetically engineered bacterium comprising energy-generating fermentation pathway |
CA3151149A Active CA3151149C (en) | 2015-10-13 | 2016-10-13 | Genetically engineered bacterium comprising energy-generating fermentation pathway |
CA3151146A Active CA3151146C (en) | 2015-10-13 | 2016-10-13 | Genetically engineered bacterium comprising energy-generating fermentation pathway |
CA3051235A Active CA3051235C (en) | 2015-10-13 | 2016-10-13 | Genetically engineered bacterium comprising energy-generating fermentation pathway |
CA3151120A Pending CA3151120A1 (en) | 2015-10-13 | 2016-10-13 | Genetically engineered bacterium comprising energy-generating fermentation pathway |
Family Applications Before (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA3051234A Active CA3051234C (en) | 2015-10-13 | 2016-10-13 | Genetically engineered bacterium comprising energy-generating fermentation pathway |
CA3001596A Active CA3001596C (en) | 2015-10-13 | 2016-10-13 | Genetically engineered bacterium comprising energy-generating fermentation pathway |
Family Applications After (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA3151146A Active CA3151146C (en) | 2015-10-13 | 2016-10-13 | Genetically engineered bacterium comprising energy-generating fermentation pathway |
CA3051235A Active CA3051235C (en) | 2015-10-13 | 2016-10-13 | Genetically engineered bacterium comprising energy-generating fermentation pathway |
CA3151120A Pending CA3151120A1 (en) | 2015-10-13 | 2016-10-13 | Genetically engineered bacterium comprising energy-generating fermentation pathway |
Country Status (13)
Country | Link |
---|---|
US (3) | US9738875B2 (ja) |
EP (2) | EP3901267A1 (ja) |
JP (2) | JP6905518B2 (ja) |
KR (2) | KR20180056785A (ja) |
CN (2) | CN108884467A (ja) |
AU (2) | AU2016339989B2 (ja) |
BR (1) | BR112018007338A2 (ja) |
CA (6) | CA3051234C (ja) |
EA (2) | EA202190729A1 (ja) |
MX (4) | MX2022003568A (ja) |
MY (1) | MY194651A (ja) |
WO (1) | WO2017066498A1 (ja) |
ZA (5) | ZA201802346B (ja) |
Families Citing this family (43)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2016042012A1 (en) * | 2014-09-17 | 2016-03-24 | Global Bioenergies | Methods for producing 3-hydroxy-3-methylbutyric acid |
US11291693B2 (en) | 2015-06-25 | 2022-04-05 | Synlogic Operating Company, Inc. | Bacteria engineered to treat metabolic diseases |
CN108884467A (zh) * | 2015-10-13 | 2018-11-23 | 朗泽科技新西兰有限公司 | 包含产能发酵途径的基因工程菌 |
GB201603915D0 (en) * | 2016-03-07 | 2016-04-20 | Chain Biotechnology Ltd | Method and microbes for the production of chiral compounds |
EA201892627A1 (ru) | 2016-05-14 | 2019-04-30 | Ланцатек, Инк. | Микроорганизм с модифицированной активностью альдегид:ферредоксин оксидоредуктазы и связанные способы |
US11539066B2 (en) | 2016-11-22 | 2022-12-27 | The Penn State Research Foundation | Devices and methods for generating electrical current from methane |
CN107177609B (zh) * | 2017-05-15 | 2020-12-11 | 大连理工大学 | 一种fruB基因过表达的重组梭菌、其构建方法及应用 |
CN107083406B (zh) * | 2017-05-27 | 2021-02-02 | 浙江华睿生物技术有限公司 | (r)-3-羟基丁酸的生产方法 |
WO2019006255A1 (en) * | 2017-06-30 | 2019-01-03 | Invista North America S.A.R.L. | METHODS, MATERIALS, SYNTHETIC HOSTS AND REAGENTS FOR HYDROCARBON BIOSYNTHESIS AND DERIVATIVES |
BR112020004591A2 (pt) | 2017-09-08 | 2020-09-08 | Lanzatech, Inc. | processo para produzir um ou mais produtos de fermentação |
US11505809B2 (en) | 2017-09-28 | 2022-11-22 | Inv Nylon Chemicals Americas Llc | Organisms and biosynthetic processes for hydrocarbon synthesis |
US20200239896A1 (en) * | 2017-09-28 | 2020-07-30 | Lanzatech, Inc. | Genetic knockouts in wood-ljungdahl microorganisms |
CA3083563A1 (en) * | 2017-11-30 | 2019-06-06 | Toray Industries, Inc. | Gene-modified microorganism for producing 3-hydroxyadipic acid, .alpha.-hydromuconic acid, and/or adipic acid, and production method for said chemical products |
US11555209B2 (en) | 2017-12-19 | 2023-01-17 | Lanzatech, Inc. | Microorganisms and methods for the biological production of ethylene glycol |
EP3743520A4 (en) * | 2018-01-23 | 2021-11-10 | Lanzatech, Inc. | TWO-STAGE FERMENTATION PROCESS FOR MANUFACTURING A PRODUCT |
WO2019152753A1 (en) * | 2018-02-01 | 2019-08-08 | Invista Textiles (U.K.) Limited | Methods and materials for the biosynthesis of beta hydroxy fatty acid anions and/or derivatives thereof and/or compounds related thereto |
WO2019157507A1 (en) | 2018-02-12 | 2019-08-15 | Lanzatech, Inc. | A process for improving carbon conversion efficiency |
JP2021520814A (ja) | 2018-04-20 | 2021-08-26 | ランザテク,インコーポレイテッド | 断続的な電解流 |
CN109251940B (zh) * | 2018-10-30 | 2020-10-16 | 浙江华睿生物技术有限公司 | 一种产β-羟基-β-甲基丁酸工程菌的构建方法 |
CN112955558A (zh) | 2018-11-19 | 2021-06-11 | 朗泽科技有限公司 | 发酵和气化的集成 |
CA3185501A1 (en) | 2019-02-08 | 2020-08-13 | Lanzatech, Inc. | Process for recovering close boiling products |
EP3942058A1 (en) | 2019-03-20 | 2022-01-26 | Global Bioenergies | Improved means and methods for producing isobutene from acetyl-coa |
FR3096373B1 (fr) * | 2019-05-24 | 2024-09-13 | Ifp Energies Now | Outil genetique optimisé pour modifier les bacteries |
EP3997235A4 (en) | 2019-07-11 | 2024-05-08 | Lanzatech, Inc. | PROCESSES FOR OPTIMIZING GAS USE |
US20220258100A1 (en) * | 2019-07-16 | 2022-08-18 | San Diego State University (SDSU) Foundation, dba San Diego State University Research Foundation | Products of manufacture and methods for methane capturing using biofiltration |
CN112280722B (zh) * | 2019-07-23 | 2022-03-08 | 清华大学 | 用于生产光学纯1,3-丁二醇的重组菌及其应用 |
CN112280723B (zh) * | 2019-07-23 | 2022-08-19 | 清华大学 | 联产1,3-丙二醇和1,3-丁二醇的重组菌及其应用 |
JP7376041B2 (ja) * | 2019-09-11 | 2023-11-08 | 公益財団法人地球環境産業技術研究機構 | 形質転換体及びそれを用いる1,3-ブタンジオールの製造方法 |
CN111089914A (zh) * | 2019-11-07 | 2020-05-01 | 河北首朗新能源科技有限公司 | 微生物发酵生产乙醇中代谢物的高效液相检测方法 |
EP4081573A1 (en) * | 2019-12-23 | 2022-11-02 | CO2BioClean GmbH | Novel bioplastics |
JP7475598B2 (ja) * | 2020-03-04 | 2024-04-30 | 大阪瓦斯株式会社 | 1,3-ブタンジオールの製造方法 |
CN111220762A (zh) * | 2020-03-24 | 2020-06-02 | 河北首朗新能源科技有限公司 | 一种乙醇梭菌蛋白中有机物的含量的检测方法及应用 |
KR20220159450A (ko) * | 2020-04-29 | 2022-12-02 | 란자테크, 인크. | 가스성 기질로부터의 β-케토아디페이트의 발효 제조 |
KR20220164810A (ko) * | 2020-06-06 | 2022-12-13 | 란자테크, 인크. | 아세토락테이트 탈카복실화효소 유전자위에 녹인이 있는 미생물 |
WO2022066997A1 (en) * | 2020-09-25 | 2022-03-31 | Lanzatech, Inc. | Recombinant microorganisms and uses therefor |
KR102443037B1 (ko) | 2020-10-23 | 2022-09-14 | 한국과학기술연구원 | 에탄올을 포함하는 합성 가스로부터 2,3-부탄디올 제조용 배지 조성물 및 이를 이용한 2,3-부탄디올 생산 방법 |
CA3195088C (en) | 2021-02-08 | 2024-04-23 | Fungmin Liew | Recombinant microorganisms and their use in the production of 3-hydroxypropionate [3-hp] |
CN115369048B (zh) * | 2021-05-17 | 2023-10-20 | 华东理工大学 | 一种产玉米黄质的解脂耶氏酵母基因工程菌及其构建方法和应用 |
TW202307202A (zh) | 2021-08-06 | 2023-02-16 | 美商朗澤科技有限公司 | 用於改良乙二醇之生物產生的微生物及方法 |
US12091648B2 (en) | 2021-11-03 | 2024-09-17 | Lanzatech, Inc. | System and method for generating bubbles in a vessel |
EP4279586A4 (en) | 2022-04-06 | 2024-02-28 | Shenzhen Bluepha Biosciences Co., Ltd. | MODIFIED MICROORGANISM EXPRESSING AN ACETOACETYL REDUCTASE COENZYME VARIANT AND METHOD FOR INCREASING PHA YIELD |
CN116121219B (zh) * | 2022-04-06 | 2023-11-28 | 深圳蓝晶生物科技有限公司 | 表达乙酰乙酰辅酶a还原酶变体的工程化微生物及提高pha产量的方法 |
US12077800B2 (en) | 2022-06-16 | 2024-09-03 | Lanzatech, Inc. | Liquid distributor system and process of liquid distribution |
Family Cites Families (41)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5593886A (en) | 1992-10-30 | 1997-01-14 | Gaddy; James L. | Clostridium stain which produces acetic acid from waste gases |
UA72220C2 (uk) | 1998-09-08 | 2005-02-15 | Байоенджініерінг Рісорсиз, Інк. | Незмішувана з водою суміш розчинник/співрозчинник для екстрагування оцтової кислоти, спосіб одержання оцтової кислоти (варіанти), спосіб анаеробного мікробного бродіння для одержання оцтової кислоти (варіанти), модифікований розчинник та спосіб його одержання |
CN1217001C (zh) * | 2002-01-04 | 2005-08-31 | 清华大学 | 一种生产d-(-)-3-羟基丁酸的方法 |
CN101432434B (zh) * | 2005-09-29 | 2015-03-11 | 布特马斯先进生物燃料公司 | 4碳醇的发酵生产 |
NZ546496A (en) | 2006-04-07 | 2008-09-26 | Lanzatech New Zealand Ltd | Gas treatment process |
US7704723B2 (en) | 2006-08-31 | 2010-04-27 | The Board Of Regents For Oklahoma State University | Isolation and characterization of novel clostridial species |
NZ553984A (en) | 2007-03-19 | 2009-07-31 | Lanzatech New Zealand Ltd | Alcohol production process |
KR101114918B1 (ko) * | 2007-08-09 | 2012-02-15 | 주식회사 엘지화학 | 재조합 미생물을 이용한 광학활성(s)-3-하이드록시부탄산 및(s)-3-하이드록시부티레이트 에스테르의 제조방법 |
KR101042242B1 (ko) * | 2007-09-07 | 2011-06-17 | 한국과학기술원 | 1,4-부탄디올 생성능을 가지는 변이체 및 이를 이용한1,4-부탄디올의 제조방법 |
EA022710B1 (ru) | 2007-11-13 | 2016-02-29 | Ланзатек Нью Зиленд Лимитед | Штамм бактерии clostridium autoethanogenum, способный продуцировать этанол и ацетат путем анаэробной ферментации субстрата, содержащего co |
JP5951990B2 (ja) * | 2008-03-27 | 2016-07-13 | ジェノマティカ, インコーポレイテッド | アジピン酸および他の化合物を生成するための微生物 |
CN102317463B (zh) | 2008-06-09 | 2014-12-03 | 蓝瑟科技纽西兰有限公司 | 厌氧微生物发酵生产丁二醇 |
LT2304040T (lt) | 2008-07-04 | 2017-11-27 | Scientist Of Fortune S.A. | Alkenų gamyba, fermentiniu būdu dekarboksilinant 3-hidroksialkano rūgštis |
WO2010022763A1 (en) * | 2008-08-25 | 2010-03-04 | Metabolic Explorer | Method for the preparation of 2-hydroxy-isobutyrate |
SG172806A1 (en) * | 2008-12-30 | 2011-08-29 | Danisco Us Inc | Methods of producing isoprene and a co-product |
EP2475775A4 (en) * | 2009-09-09 | 2013-03-20 | Genomatica Inc | MICROORGANISMS AND METHOD FOR THE SIMULTANEOUS PREPARATION OF ISOPROPANOL AND PRIMARY ALCOHOLS, DIOLES AND ACIDS |
EP2295593A1 (en) | 2009-09-15 | 2011-03-16 | Philippe Marliere | Method for the enymatic production of 3-hydroxy-3-methylbutyric acid from acetone and acetyl-CoA |
KR20120120493A (ko) * | 2009-12-10 | 2012-11-01 | 게노마티카 인코포레이티드 | 합성 가스 또는 기타 가스상 탄소원 및 메탄올을 1,3-부탄디올로 변환하는 방법 및 변환용 유기체 |
EP2336341A1 (en) | 2009-12-21 | 2011-06-22 | Philippe Marliere | Method for producing an alkene comprising the step of converting an alcohol by an enzymatic dehydration step |
US8048661B2 (en) * | 2010-02-23 | 2011-11-01 | Genomatica, Inc. | Microbial organisms comprising exogenous nucleic acids encoding reductive TCA pathway enzymes |
WO2011112103A1 (en) | 2010-03-10 | 2011-09-15 | Lanzatech New Zealand Limited | Acid production by fermentation |
US20120045807A1 (en) | 2010-08-19 | 2012-02-23 | Lanzatech New Zealand Limited | Process for producing chemicals using microbial fermentation of substrates comprising carbon monoxide |
US20130157322A1 (en) | 2010-08-26 | 2013-06-20 | Lanzatech New Zealand Limited | Process for producing ethanol and ethylene via fermentation |
US20110236941A1 (en) | 2010-10-22 | 2011-09-29 | Lanzatech New Zealand Limited | Recombinant microorganism and methods of production thereof |
US8271519B2 (en) | 2010-11-01 | 2012-09-18 | Sas Institute Inc. | Systems and methods for fast remote data access from a spreadsheet |
ES2702621T3 (es) * | 2011-02-25 | 2019-03-04 | Lanzatech New Zealand Ltd | Microorganismos recombinantes y sus usos |
US9365868B2 (en) | 2011-02-25 | 2016-06-14 | Lanzatech New Zealand Limited | Fermentation process for producing isopropanol using a recombinant microorganism |
MY165107A (en) | 2011-09-08 | 2018-02-28 | Lanzatech New Zealand Ltd | A fermentation process |
AU2013215706B2 (en) | 2012-01-31 | 2014-03-27 | Lanzatech Nz, Inc. | Recombinant microorganisms and methods of use thereof |
WO2013180581A1 (en) | 2012-05-30 | 2013-12-05 | Lanzatech New Zealand Limited | Recombinant microorganisms and uses therefor |
US20130323820A1 (en) * | 2012-06-01 | 2013-12-05 | Lanzatech New Zealand Limited | Recombinant microorganisms and uses therefor |
JP6381525B2 (ja) | 2012-06-08 | 2018-08-29 | ランザテク・ニュージーランド・リミテッド | 組換え微生物およびその使用 |
US9347076B2 (en) | 2012-06-21 | 2016-05-24 | Lanzatech New Zealand Limited | Recombinant microorganisms that make biodiesel |
EP2890778B1 (en) | 2012-08-28 | 2019-05-01 | Lanzatech New Zealand Limited | Recombinant microorganisms and uses therefor |
US8787096B1 (en) | 2013-01-16 | 2014-07-22 | Qualcomm Incorporated | N-well switching circuit |
EP2813575A1 (de) * | 2013-06-14 | 2014-12-17 | Evonik Industries AG | Verfahren zur Herstellung von organischen Verbindungen aus Knallgas und CO2 über Acetoacetyl-CoA als Zwischenprodukt |
PL3077501T3 (pl) * | 2013-12-03 | 2022-01-31 | Genomatica, Inc. | Mikroorganizmy i sposoby poprawy wydajności produktu na metanolu z użyciem syntezy acetylo-coa |
CA2931627A1 (en) | 2013-12-03 | 2015-06-11 | Lanzatech New Zealand Limited | Microorganisms and methods for the production of ketones |
EP3090043B1 (en) * | 2013-12-31 | 2022-02-02 | Global Bioenergies | 3-hydroxyisovalerate (hiv) synthase variants |
BR112017003733A2 (pt) | 2014-09-03 | 2017-12-05 | Global Bioenergies | ?micro-organismo recombinante que produz alcenos a partir de acetil-coa?. |
CN108884467A (zh) * | 2015-10-13 | 2018-11-23 | 朗泽科技新西兰有限公司 | 包含产能发酵途径的基因工程菌 |
-
2016
- 2016-10-13 CN CN201680067398.8A patent/CN108884467A/zh active Pending
- 2016-10-13 CA CA3051234A patent/CA3051234C/en active Active
- 2016-10-13 CA CA3001596A patent/CA3001596C/en active Active
- 2016-10-13 KR KR1020187013448A patent/KR20180056785A/ko not_active Application Discontinuation
- 2016-10-13 CN CN202110295941.2A patent/CN113322220A/zh active Pending
- 2016-10-13 EA EA202190729A patent/EA202190729A1/ru unknown
- 2016-10-13 US US15/293,191 patent/US9738875B2/en active Active
- 2016-10-13 BR BR112018007338A patent/BR112018007338A2/pt unknown
- 2016-10-13 MY MYPI2020005284A patent/MY194651A/en unknown
- 2016-10-13 EP EP21164171.7A patent/EP3901267A1/en active Pending
- 2016-10-13 JP JP2018519408A patent/JP6905518B2/ja active Active
- 2016-10-13 MX MX2022003568A patent/MX2022003568A/es unknown
- 2016-10-13 EA EA201890952A patent/EA201890952A1/ru unknown
- 2016-10-13 CA CA3151149A patent/CA3151149C/en active Active
- 2016-10-13 CA CA3151146A patent/CA3151146C/en active Active
- 2016-10-13 CA CA3051235A patent/CA3051235C/en active Active
- 2016-10-13 AU AU2016339989A patent/AU2016339989B2/en active Active
- 2016-10-13 KR KR1020217008961A patent/KR20210037002A/ko active Application Filing
- 2016-10-13 WO PCT/US2016/056926 patent/WO2017066498A1/en active Application Filing
- 2016-10-13 MX MX2023003058A patent/MX2023003058A/es unknown
- 2016-10-13 CA CA3151120A patent/CA3151120A1/en active Pending
- 2016-10-13 MX MX2018004642A patent/MX2018004642A/es unknown
- 2016-10-13 EP EP16856229.6A patent/EP3362567A4/en active Pending
-
2017
- 2017-07-25 US US15/658,668 patent/US9957531B1/en active Active
-
2018
- 2018-03-15 US US15/922,451 patent/US10316337B2/en active Active
- 2018-04-10 ZA ZA2018/02346A patent/ZA201802346B/en unknown
- 2018-04-13 MX MX2021003330A patent/MX2021003330A/es unknown
-
2019
- 2019-07-08 ZA ZA2019/04454A patent/ZA201904454B/en unknown
- 2019-07-08 ZA ZA2019/04455A patent/ZA201904455B/en unknown
-
2021
- 2021-01-29 ZA ZA2021/00646A patent/ZA202100646B/en unknown
- 2021-03-26 ZA ZA2021/02053A patent/ZA202102053B/en unknown
- 2021-05-12 AU AU2021203008A patent/AU2021203008B9/en active Active
- 2021-06-25 JP JP2021106017A patent/JP2021166530A/ja active Pending
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US10316337B2 (en) | Genetically engineered bacterium for the production of isobutylene | |
CA2985481C (en) | Genetically engineered microorganisms for the production of chorismate-derived products | |
CA2882276C (en) | Recombinant microorganisms expressing a stereospecific diol dehydratase enzyme | |
US20090111154A1 (en) | Butanol production by recombinant microorganisms | |
CA3079761A1 (en) | Microorganisms and methods for the biological production of ethylene glycol | |
WO2012135731A2 (en) | Alcohol production from recombinant microorganisms | |
US20210292732A1 (en) | Fermentative production of 2-phenylethanol from gaseous substrates | |
US9434963B2 (en) | Process for butanol production | |
TWI811184B (zh) | 包括產能醱酵路徑之經基因工程改造之細菌 | |
EA043734B1 (ru) | Генетически сконструированная бактерия, содержащая энергогенерирующий ферментативный путь | |
TW202128984A (zh) | 包括產能醱酵路徑之經基因工程改造之細菌 | |
CA3228407A1 (en) | Microbial fermentation for the production of isoprenoid alcohols and derivatives |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request |
Effective date: 20220303 |
|
EEER | Examination request |
Effective date: 20220303 |
|
EEER | Examination request |
Effective date: 20220303 |
|
EEER | Examination request |
Effective date: 20220303 |
|
EEER | Examination request |
Effective date: 20220303 |
|
EEER | Examination request |
Effective date: 20220303 |
|
EEER | Examination request |
Effective date: 20220303 |
|
EEER | Examination request |
Effective date: 20220303 |
|
EEER | Examination request |
Effective date: 20220303 |