BR112017003733A2 - ?micro-organismo recombinante que produz alcenos a partir de acetil-coa?. - Google Patents

?micro-organismo recombinante que produz alcenos a partir de acetil-coa?.

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Publication number
BR112017003733A2
BR112017003733A2 BR112017003733A BR112017003733A BR112017003733A2 BR 112017003733 A2 BR112017003733 A2 BR 112017003733A2 BR 112017003733 A BR112017003733 A BR 112017003733A BR 112017003733 A BR112017003733 A BR 112017003733A BR 112017003733 A2 BR112017003733 A2 BR 112017003733A2
Authority
BR
Brazil
Prior art keywords
coa
conversion
catalyze
enzymes
acetyl
Prior art date
Application number
BR112017003733A
Other languages
English (en)
Inventor
Guentner Bernhard
Original Assignee
Global Bioenergies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Global Bioenergies filed Critical Global Bioenergies
Publication of BR112017003733A2 publication Critical patent/BR112017003733A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

a presente invenção refere-se a um micro-organismo recombinante que compreende enzimas endógenas que convertem co e/ou co2 em acetil-coa. o micro-organismo recombinante contém uma sequência de ácidos nucleicos heterólogos que codificam uma ou mais enzimas que permitem a conversão de acetil-coa em um alceno com uma cadeia principal de 1 a 5 átomos de carbono. a sequência de ácidos nucleicos heterólogos compreende uma ou mais sequências de codificação que codificam uma ou mais enzimas que catalisam a conversão de acetil-coa em crotonil-coa e que catalisam, ainda, a conversão de crotonil-coa em um alceno; ou uma ou mais sequências de codificação que codificam uma ou mais enzimas que catalisam a conversão de acetil-coa em 3-metilcrotonil-coa e que catalisam, ainda, a conversão de 3-metilcrotonil-coa em um alceno; ou uma ou mais sequências de codificação que codificam uma ou mais enzimas que catalisam a conversão de acetil-coa em propionil-coa e que catalisam, ainda, a conversão de propionil-coa em um alceno. cada sequência de codificação está ligada operacionalmente a um promotor transcricional.
BR112017003733A 2014-09-03 2015-09-03 ?micro-organismo recombinante que produz alcenos a partir de acetil-coa?. BR112017003733A2 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201462045083P 2014-09-03 2014-09-03
US201462047827P 2014-09-09 2014-09-09
EP14186574 2014-09-26
EP14186690 2014-09-26
PCT/EP2015/070194 WO2016034691A1 (en) 2014-09-03 2015-09-03 Recombinant microorganism producing alkenes from acetyl-coa

Publications (1)

Publication Number Publication Date
BR112017003733A2 true BR112017003733A2 (pt) 2017-12-05

Family

ID=55439158

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017003733A BR112017003733A2 (pt) 2014-09-03 2015-09-03 ?micro-organismo recombinante que produz alcenos a partir de acetil-coa?.

Country Status (6)

Country Link
US (1) US20170283809A1 (pt)
EP (1) EP3189130A1 (pt)
JP (1) JP2017525380A (pt)
CN (1) CN107075464A (pt)
BR (1) BR112017003733A2 (pt)
WO (1) WO2016034691A1 (pt)

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BR112016002626A2 (pt) 2013-08-05 2017-09-12 Invista Tech Sarl método para sintetizar isobuteno e hospedeiro recombinante
MX2018004642A (es) 2015-10-13 2019-04-15 Lanzatech New Zealand Ltd Bacteria diseñada por ingeniería genética que comprende una trayectoria de fermentación que genera energía.
EP3377639A2 (en) 2015-11-17 2018-09-26 Global Bioenergies Methods for producing isobutene from 3-methylcrotonic acid
DK3484996T3 (da) * 2016-07-14 2020-12-07 Basf Se Fermenteringsmedium, der omfatter et chelateringsmiddel
CA3104645A1 (en) 2018-07-27 2020-01-30 Global Bioenergies Method for producing fructose-6-phosphate from dihydroxyacetone phosphate and glyceraldehyde-3-phosphate
CN110964678B (zh) * 2018-09-29 2022-11-11 中国科学院青岛生物能源与过程研究所 一种合成法尼烯的基因工程菌及其构建方法与应用
WO2020188033A1 (en) 2019-03-20 2020-09-24 Global Bioenergies Improved means and methods for producing isobutene from acetyl-coa
WO2021063958A1 (en) 2019-09-30 2021-04-08 Global Bioenergies Improved means and methods for increasing the yield of acetyl-coa from glucose
CN112481320B (zh) * 2020-12-09 2022-07-05 江南大学 一种催化效率高的制备(-)γ-内酰胺的方法
WO2023110750A1 (en) * 2021-12-13 2023-06-22 Global Bioenergies Acyl-coa hydrolase variants

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BR112013032516A2 (pt) * 2011-06-17 2017-03-01 Invista Tech Sarl método de biosintetizar butadieno e método para produzir butadieno
PL2732031T3 (pl) * 2011-07-12 2016-03-31 Scientist Of Fortune Sa Rekombinowany mikroorganizm do wytwarzania użytecznych metabolitów
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Also Published As

Publication number Publication date
EP3189130A1 (en) 2017-07-12
WO2016034691A1 (en) 2016-03-10
US20170283809A1 (en) 2017-10-05
CN107075464A (zh) 2017-08-18
JP2017525380A (ja) 2017-09-07

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B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements