CA2977578C - Cell culture method for mesenchymal stem cells - Google Patents

Cell culture method for mesenchymal stem cells

Info

Publication number
CA2977578C
CA2977578C CA2977578A CA2977578A CA2977578C CA 2977578 C CA2977578 C CA 2977578C CA 2977578 A CA2977578 A CA 2977578A CA 2977578 A CA2977578 A CA 2977578A CA 2977578 C CA2977578 C CA 2977578C
Authority
CA
Canada
Prior art keywords
cell culture
stem cells
culture process
mesenchymatous
mesenchymatous stem
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2977578A
Other languages
English (en)
French (fr)
Other versions
CA2977578A1 (en
Inventor
Paul Simmons
Colby Suire
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mesoblast International SARL
Original Assignee
Mesoblast International SARL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2015900752A external-priority patent/AU2015900752A0/en
Application filed by Mesoblast International SARL filed Critical Mesoblast International SARL
Publication of CA2977578A1 publication Critical patent/CA2977578A1/en
Application granted granted Critical
Publication of CA2977578C publication Critical patent/CA2977578C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/135—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
    • C12N2502/115—Platelets, megakaryocytes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CA2977578A 2015-03-04 2016-03-04 Cell culture method for mesenchymal stem cells Active CA2977578C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
AU2015900752 2015-03-04
AU2015900752A AU2015900752A0 (en) 2015-03-04 Cell culture method
AU2015900777 2015-03-05
AU2015900777A AU2015900777A0 (en) 2015-03-05 Cell culture method
PCT/EP2016/054640 WO2016139340A1 (en) 2015-03-04 2016-03-04 Cell culture method for mesenchymal stem cells

Publications (2)

Publication Number Publication Date
CA2977578A1 CA2977578A1 (en) 2016-09-09
CA2977578C true CA2977578C (en) 2024-09-10

Family

ID=55453191

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2977578A Active CA2977578C (en) 2015-03-04 2016-03-04 Cell culture method for mesenchymal stem cells

Country Status (10)

Country Link
US (4) US20180037867A1 (enExample)
EP (2) EP3265554B1 (enExample)
JP (3) JP6885870B2 (enExample)
KR (1) KR102635212B1 (enExample)
CN (2) CN113957043A (enExample)
AU (2) AU2016227607B2 (enExample)
BR (1) BR112017018885A2 (enExample)
CA (1) CA2977578C (enExample)
SG (1) SG11201706886PA (enExample)
WO (1) WO2016139340A1 (enExample)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2334785B2 (en) * 2008-09-16 2019-08-14 Mayo Foundation For Medical Education And Research Compositions containing platelet contents
US20120087868A1 (en) 2010-10-08 2012-04-12 Gabriele Todd Nanoparticle-loaded cells
EP4328299A3 (en) 2014-05-16 2024-05-29 Mayo Foundation for Medical Education and Research Cell culture media compositions for primary cells
US20180037867A1 (en) 2015-03-04 2018-02-08 Mesoblast International Sàrl Cell culture method for mesenchymal stem cells
CN112004923A (zh) 2017-11-22 2020-11-27 迈索布拉斯特国际有限公司 细胞组合物及治疗方法
CN108251358B (zh) * 2017-12-15 2021-10-26 广东药科大学 一种相同供体来源人间充质干细胞的多批次原代分离方法
US20210115395A1 (en) * 2018-03-28 2021-04-22 Pluristem Ltd. Methods for expanding adherent stromal cells and cells obtained thereby
CN108624554B (zh) * 2018-05-14 2021-09-14 山东大学 一种非基因修饰光操控双向调节人脐带间充质干细胞增殖的方法
WO2020060809A1 (en) * 2018-09-17 2020-03-26 Rejuvablast LLC Combination grafts for tissue repair or regeneration applications
WO2020067443A1 (ja) * 2018-09-27 2020-04-02 テルモ株式会社 体細胞のシート化方法
CA3181611A1 (en) * 2020-06-11 2021-12-16 Paul Simmons 3d culture of mesenchymal lineage precursor or stem cells
CN117396077A (zh) * 2021-03-10 2024-01-12 寺崎生物医学创新研究所 用于实验室生长肉和其他应用的生长因子
EP4304383A4 (en) * 2021-03-10 2025-02-26 Terasaki Institute For Biomedical Innovation GROWTH FACTORS FOR LABORATORY-GROWN MEAT AND OTHER APPLICATIONS
EP4630536A1 (en) 2022-12-09 2025-10-15 Mesoblast International Sàrl Conditioned media and use of the same
JP2025538783A (ja) 2022-12-09 2025-11-28 メゾブラスト・インターナショナル・エスアーエールエル 事前承認された組成物及び細胞培養方法
CN120513094A (zh) 2022-12-09 2025-08-19 迈索布拉斯特国际有限公司 治疗患有持续性炎症的受试者的心力衰竭的方法
WO2024121819A1 (en) 2022-12-09 2024-06-13 Mesoblast International Sarl Method of treating inflammation with cellular compositions
WO2024225602A1 (ko) * 2023-04-24 2024-10-31 파미셀 주식회사 뉴클레오시드 함유 세포 배양배지 및 이를 이용한 세포 배양 방법
WO2025046491A1 (en) 2023-08-28 2025-03-06 Mesoblast International Sàrl Third line treatment for gvhd
WO2025062365A1 (en) 2023-09-22 2025-03-27 Mesoblast International Sárl Mesenchymal lineage precursor or stem cells (mlpscs) for use in methods of treating congenital heart disease

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5486359A (en) 1990-11-16 1996-01-23 Osiris Therapeutics, Inc. Human mesenchymal stem cells
DE59209946D1 (de) 1991-08-12 2002-03-28 Storz Karl Gmbh & Co Kg Hochfrequenzchirurgiegenerator mit einer einstellbaren Ausgangsleistung
US5814464A (en) 1994-10-07 1998-09-29 Regeneron Pharma Nucleic acids encoding TIE-2 ligand-2
US6204053B1 (en) 1994-11-08 2001-03-20 Diacrin, Inc. Porcine cortical cells and their use in treatment of neurological deficits due to neurodegenerative diseases
US6277372B1 (en) 1994-11-08 2001-08-21 Diacrin, Inc. Porcine neural cells and their use in treatment of neurological deficits due to neurodegenerative diseases
EP0821728B1 (en) 1995-04-06 2004-08-11 Regeneron Pharmaceuticals, Inc. Tie-2 ligands, methods of making and uses thereof
EP0986635A4 (en) 1997-01-10 2001-11-07 Life Technologies Inc SERUM SUBSTITUTE FOR EMBRYONIC STEM CELLS
US5968829A (en) 1997-09-05 1999-10-19 Cytotherapeutics, Inc. Human CNS neural stem cells
CA2348687A1 (en) * 1998-11-09 2000-05-18 Consorzio Per La Gestione Del Centro Di Biotecnologie Avanzate Serum free medium for chondrocyte-like cells
AU2003901668A0 (en) 2003-03-28 2003-05-01 Medvet Science Pty. Ltd. Non-haemopoietic precursor cells
AU2118501A (en) 1999-10-20 2001-04-30 Stemcells, Inc. Methods for inducing in vivo proliferation and migration of transplanted progenitor cells in the brain
US7439064B2 (en) 2000-03-09 2008-10-21 Wicell Research Institute, Inc. Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
NZ527849A (en) 2001-02-14 2006-09-29 Anthrogenesis Corp Post-partum mammalian placenta, its use and placental stem cells therefrom
NZ528035A (en) 2001-02-14 2005-07-29 Robert J Hariri Renovation and repopulation of decellularized tissues and cadaveric organs by stem cells
IL160507A0 (en) 2001-08-24 2004-07-25 Advanced Cell Tech Inc Screening assays for identifying differentiation-inducing agents and production of differentiated cells for cell therapy
JP2005517402A (ja) 2002-02-13 2005-06-16 アンスロジェネシス コーポレーション 分娩後の哺乳動物胎盤由来の胚様幹細胞、ならびに該細胞の用途および該細胞を用いる治療法
CN1662643A (zh) 2002-05-28 2005-08-31 贝克顿·迪金森公司 人腺泡细胞的扩增和转分化
CN1668324A (zh) 2002-05-28 2005-09-14 诺沃塞尔公司 用于胰岛素产生细胞的方法、组合物及生长与分化因子
US8491883B2 (en) 2003-06-27 2013-07-23 Advanced Technologies And Regenerative Medicine, Llc Treatment of amyotrophic lateral sclerosis using umbilical derived cells
CA2530421C (en) 2003-06-27 2015-04-21 Ethicon, Incorporated Repair and regeneration of ocular tissue using postpartum-derived cells
KR100931887B1 (ko) 2004-02-26 2009-12-15 리라이언스 라이프 사이언시스 프라이빗. 리미티드 각막 윤부에서 유도된 다능성 배아 유사 줄기 세포, 분리 방법 및 이들의 용도
WO2006071777A2 (en) 2004-12-23 2006-07-06 Ethicon Incorporated Soft tissue repair and regeneration using postpartum-derived cells and cell products
JP5340599B2 (ja) 2004-12-23 2013-11-13 エシコン・インコーポレイテッド 臍帯組織由来産褥細胞ならびにその製造方法および使用方法
WO2006071773A2 (en) 2004-12-23 2006-07-06 Ethicon Incoporated Treatment of osteochondral diseases using postpartum-derived cells and products thereof
EP1835924B1 (en) 2004-12-23 2013-08-21 Ethicon, Incorporated Treatment of parkinson's disease and related disorders using postpartum derived cells
BRPI0617084A2 (pt) 2005-09-02 2011-07-12 Agency Science Tech & Res método, linhagem de célula progenitora, célula diferenciada e método para gerar uma célula diferenciada de uma célula-tronco (es) embrionária
US9074176B2 (en) 2006-01-13 2015-07-07 Two Cells Co., Ltd. Culture medium additive for use in serum-free culturing of animal cell, kit and use thereof
WO2007099337A1 (en) * 2006-03-01 2007-09-07 Cartela R&D Ab Expansion and differentiation of mesenchymal stem cells
EP2054506A1 (en) * 2006-08-15 2009-05-06 Agency for Science, Technology and Research Mesenchymal stem cell conditioned medium
JP2008148643A (ja) * 2006-12-19 2008-07-03 Olympus Corp 無血清培地および幹細胞の培養方法
HRP20130765T1 (hr) 2007-02-12 2013-10-25 Anthrogenesis Corporation Lijeäśenje protuupalnih bolesti putem matiäśnih stanica posteljice
WO2008151390A1 (en) 2007-06-15 2008-12-18 Australian Stem Cell Centre Ltd Differentiation of human embryonic stem cells
US20110236971A2 (en) * 2007-09-25 2011-09-29 Maksym Vodyanyk Generation of Clonal Mesenchymal Progenitors and Mesenchymal Stem Cell Lines Under Serum-Free Conditions
KR101224588B1 (ko) * 2008-01-24 2013-01-22 삼성전자주식회사 멀티포인트 스트록을 감지하기 위한 ｕｉ 제공방법 및 이를적용한 멀티미디어 기기
WO2009158634A2 (en) 2008-06-27 2009-12-30 Zimmer Orthobiologics, Inc. Scaffold coated and/or impregnated with at least one bioactive agent for tissue repair and other medical applications
US20100055078A1 (en) * 2008-08-15 2010-03-04 The Government of the U.S.A, as represented by the Department of Veterans Affairs Methods of making a transplantable bone repair system using multipotent stem cells
JP5660572B2 (ja) 2008-11-11 2015-01-28 国立大学法人広島大学 分化誘導培地用添加剤およびその利用
US20100158878A1 (en) 2008-12-23 2010-06-24 Alexandra Capela Target populations of oligodendrocyte precursor cells and methods of making and using same
US10894944B2 (en) 2009-04-10 2021-01-19 Monash University Cell culture media
JP5804385B2 (ja) * 2010-03-10 2015-11-04 株式会社ツーセル 間葉系幹細胞を含む細胞製剤及びその製造方法
CN103068969A (zh) * 2010-06-17 2013-04-24 思迪公司 化学成分确定的无血清细胞培养基
MX364742B (es) * 2011-01-12 2019-05-07 Tsuneo Kido Metodo de cultivo para obtener y mantener una poblacion pura o enriquecida de celulas no diferenciadas neurales y/o celulas progenitoras neurales de mamifero que con propensas a diferenciarse en celulas del linaje oligodendrocitico in vitro.
KR101523040B1 (ko) * 2012-06-26 2015-05-26 라정찬 고농도의 줄기세포 제조방법
WO2014082685A1 (en) * 2012-11-30 2014-06-05 Swiss Stem Cell Foundation Serum-free medium for human mesenchymal stem cells
CN103396984B (zh) * 2013-07-25 2016-02-10 宗兆文 一种利用皮肤来源的祖细胞制备组织工程脊髓的方法
US20180037867A1 (en) 2015-03-04 2018-02-08 Mesoblast International Sàrl Cell culture method for mesenchymal stem cells

Also Published As

Publication number Publication date
CA2977578A1 (en) 2016-09-09
EP3265554B1 (en) 2021-11-17
KR20170120627A (ko) 2017-10-31
US20240191200A1 (en) 2024-06-13
EP3265554A1 (en) 2018-01-10
JP7410899B2 (ja) 2024-01-10
AU2021254538A1 (en) 2021-11-18
SG11201706886PA (en) 2017-09-28
AU2016227607A1 (en) 2017-09-07
US20250129337A1 (en) 2025-04-24
JP2021090463A (ja) 2021-06-17
US20200040303A1 (en) 2020-02-06
BR112017018885A2 (pt) 2018-04-17
US12215352B2 (en) 2025-02-04
US11685899B2 (en) 2023-06-27
JP2024001179A (ja) 2024-01-09
JP2018506986A (ja) 2018-03-15
AU2016227607B2 (en) 2021-09-30
AU2021254538B2 (en) 2024-01-18
WO2016139340A1 (en) 2016-09-09
JP6885870B2 (ja) 2021-06-16
CN113957043A (zh) 2022-01-21
CN107567494A (zh) 2018-01-09
EP4008775A1 (en) 2022-06-08
US20180037867A1 (en) 2018-02-08
KR102635212B1 (ko) 2024-02-13

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