CA2977578C - Cell culture method for mesenchymal stem cells - Google Patents

Cell culture method for mesenchymal stem cells

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Publication number
CA2977578C
CA2977578C CA2977578A CA2977578A CA2977578C CA 2977578 C CA2977578 C CA 2977578C CA 2977578 A CA2977578 A CA 2977578A CA 2977578 A CA2977578 A CA 2977578A CA 2977578 C CA2977578 C CA 2977578C
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CA
Canada
Prior art keywords
pdgf
cell culture
culture medium
level
fgf2
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Application number
CA2977578A
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English (en)
French (fr)
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CA2977578A1 (en
Inventor
Paul Simmons
Colby SUIRE
Original Assignee
Mesoblast International SARL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
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Priority claimed from AU2015900752A external-priority patent/AU2015900752A0/en
Application filed by Mesoblast International SARL filed Critical Mesoblast International SARL
Publication of CA2977578A1 publication Critical patent/CA2977578A1/en
Application granted granted Critical
Publication of CA2977578C publication Critical patent/CA2977578C/en
Active legal-status Critical Current
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/135—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
    • C12N2502/115—Platelets, megakaryocytes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2977578A 2015-03-04 2016-03-04 Cell culture method for mesenchymal stem cells Active CA2977578C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
AU2015900752 2015-03-04
AU2015900752A AU2015900752A0 (en) 2015-03-04 Cell culture method
AU2015900777A AU2015900777A0 (en) 2015-03-05 Cell culture method
AU2015900777 2015-03-05
PCT/EP2016/054640 WO2016139340A1 (en) 2015-03-04 2016-03-04 Cell culture method for mesenchymal stem cells

Publications (2)

Publication Number Publication Date
CA2977578A1 CA2977578A1 (en) 2016-09-09
CA2977578C true CA2977578C (en) 2024-09-10

Family

ID=55453191

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2977578A Active CA2977578C (en) 2015-03-04 2016-03-04 Cell culture method for mesenchymal stem cells

Country Status (10)

Country Link
US (4) US20180037867A1 (enExample)
EP (2) EP4008775A1 (enExample)
JP (3) JP6885870B2 (enExample)
KR (1) KR102635212B1 (enExample)
CN (2) CN113957043A (enExample)
AU (2) AU2016227607B2 (enExample)
BR (1) BR112017018885A2 (enExample)
CA (1) CA2977578C (enExample)
SG (1) SG11201706886PA (enExample)
WO (1) WO2016139340A1 (enExample)

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WO2019186471A1 (en) * 2018-03-28 2019-10-03 Pluristem Ltd. Methods for expanding adherent stromal cells and cells obtained thereby
CN108624554B (zh) * 2018-05-14 2021-09-14 山东大学 一种非基因修饰光操控双向调节人脐带间充质干细胞增殖的方法
US11364323B2 (en) 2018-09-17 2022-06-21 Rejuvablast LLC Combination grafts for tissue repair or regeneration applications
JPWO2020067443A1 (ja) * 2018-09-27 2021-08-30 テルモ株式会社 体細胞のシート化方法
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WO2021176001A1 (en) 2020-03-05 2021-09-10 Mesoblast International Sàrl Method for treating inflammatory lung diseases using mesenchymal lineage precursor or stem cells
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US20240352423A1 (en) * 2021-03-10 2024-10-24 Terasaki Institute For Biomedical Innovation Methods and systems of producing products such as animal derived products
CN117529234A (zh) * 2021-03-10 2024-02-06 寺崎生物医学创新研究所 生产产品例如动物源性产品的方法和系统
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JP2025538783A (ja) 2022-12-09 2025-11-28 メゾブラスト・インターナショナル・エスアーエールエル 事前承認された組成物及び細胞培養方法
AU2023389700A1 (en) 2022-12-09 2025-07-10 Mesoblast International Sarl Method of treating heart failure in subjects with persistent inflammation
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WO2024225602A1 (ko) * 2023-04-24 2024-10-31 파미셀 주식회사 뉴클레오시드 함유 세포 배양배지 및 이를 이용한 세포 배양 방법
KR20260061190A (ko) 2023-08-28 2026-05-06 메조블라스트 인터내셔널 에스에이알엘 GvHD에 대한 3차 치료
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Also Published As

Publication number Publication date
US20250129337A1 (en) 2025-04-24
EP4008775A1 (en) 2022-06-08
CN113957043A (zh) 2022-01-21
JP2021090463A (ja) 2021-06-17
AU2016227607A1 (en) 2017-09-07
AU2021254538B2 (en) 2024-01-18
JP2018506986A (ja) 2018-03-15
JP6885870B2 (ja) 2021-06-16
KR20170120627A (ko) 2017-10-31
EP3265554B1 (en) 2021-11-17
BR112017018885A2 (pt) 2018-04-17
CN107567494A (zh) 2018-01-09
WO2016139340A1 (en) 2016-09-09
JP2024001179A (ja) 2024-01-09
EP3265554A1 (en) 2018-01-10
US11685899B2 (en) 2023-06-27
KR102635212B1 (ko) 2024-02-13
CA2977578A1 (en) 2016-09-09
US20240191200A1 (en) 2024-06-13
US20200040303A1 (en) 2020-02-06
AU2021254538A1 (en) 2021-11-18
JP7410899B2 (ja) 2024-01-10
US12215352B2 (en) 2025-02-04
SG11201706886PA (en) 2017-09-28
US20180037867A1 (en) 2018-02-08
AU2016227607B2 (en) 2021-09-30

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