AU2016227607B2 - Cell culture method for mesenchymal stem cells - Google Patents

Cell culture method for mesenchymal stem cells Download PDF

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Publication number
AU2016227607B2
AU2016227607B2 AU2016227607A AU2016227607A AU2016227607B2 AU 2016227607 B2 AU2016227607 B2 AU 2016227607B2 AU 2016227607 A AU2016227607 A AU 2016227607A AU 2016227607 A AU2016227607 A AU 2016227607A AU 2016227607 B2 AU2016227607 B2 AU 2016227607B2
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cell culture
pdgf
culture medium
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AU2016227607A1 (en
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Paul Simmons
Colby SUIRE
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Mesoblast International SARL
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Mesoblast International SARL
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/135—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
    • C12N2502/115—Platelets, megakaryocytes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2016227607A 2015-03-04 2016-03-04 Cell culture method for mesenchymal stem cells Active AU2016227607B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2021254538A AU2021254538B2 (en) 2015-03-04 2021-10-19 Cell culture method for mesenchymal stem cells

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
AU2015900752 2015-03-04
AU2015900752A AU2015900752A0 (en) 2015-03-04 Cell culture method
AU2015900777A AU2015900777A0 (en) 2015-03-05 Cell culture method
AU2015900777 2015-03-05
PCT/EP2016/054640 WO2016139340A1 (en) 2015-03-04 2016-03-04 Cell culture method for mesenchymal stem cells

Related Child Applications (1)

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AU2021254538A Division AU2021254538B2 (en) 2015-03-04 2021-10-19 Cell culture method for mesenchymal stem cells

Publications (2)

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AU2016227607A1 AU2016227607A1 (en) 2017-09-07
AU2016227607B2 true AU2016227607B2 (en) 2021-09-30

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AU2016227607A Active AU2016227607B2 (en) 2015-03-04 2016-03-04 Cell culture method for mesenchymal stem cells
AU2021254538A Active AU2021254538B2 (en) 2015-03-04 2021-10-19 Cell culture method for mesenchymal stem cells

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AU2021254538A Active AU2021254538B2 (en) 2015-03-04 2021-10-19 Cell culture method for mesenchymal stem cells

Country Status (10)

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US (4) US20180037867A1 (enExample)
EP (2) EP4008775A1 (enExample)
JP (3) JP6885870B2 (enExample)
KR (1) KR102635212B1 (enExample)
CN (2) CN113957043A (enExample)
AU (2) AU2016227607B2 (enExample)
BR (1) BR112017018885A2 (enExample)
CA (1) CA2977578C (enExample)
SG (1) SG11201706886PA (enExample)
WO (1) WO2016139340A1 (enExample)

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WO2019102268A1 (en) 2017-11-22 2019-05-31 Mesoblast International Sarl Cellular compositions and methods of treatment i
CN108251358B (zh) * 2017-12-15 2021-10-26 广东药科大学 一种相同供体来源人间充质干细胞的多批次原代分离方法
WO2019186471A1 (en) * 2018-03-28 2019-10-03 Pluristem Ltd. Methods for expanding adherent stromal cells and cells obtained thereby
CN108624554B (zh) * 2018-05-14 2021-09-14 山东大学 一种非基因修饰光操控双向调节人脐带间充质干细胞增殖的方法
US11364323B2 (en) 2018-09-17 2022-06-21 Rejuvablast LLC Combination grafts for tissue repair or regeneration applications
JPWO2020067443A1 (ja) * 2018-09-27 2021-08-30 テルモ株式会社 体細胞のシート化方法
US12702683B2 (en) 2019-01-03 2026-08-11 Mesoblast International Sárl Method for improving visual acuity
WO2021176001A1 (en) 2020-03-05 2021-09-10 Mesoblast International Sàrl Method for treating inflammatory lung diseases using mesenchymal lineage precursor or stem cells
CA3181611A1 (en) * 2020-06-11 2021-12-16 Paul Simmons 3d culture of mesenchymal lineage precursor or stem cells
US20240352423A1 (en) * 2021-03-10 2024-10-24 Terasaki Institute For Biomedical Innovation Methods and systems of producing products such as animal derived products
CN117529234A (zh) * 2021-03-10 2024-02-06 寺崎生物医学创新研究所 生产产品例如动物源性产品的方法和系统
WO2024121821A1 (en) 2022-12-09 2024-06-13 Mesoblast International Sarl Conditioned media and use of the same
EP4630018A1 (en) 2022-12-09 2025-10-15 Mesoblast International Sàrl Method of treating inflammation with cellular compositions
JP2025538783A (ja) 2022-12-09 2025-11-28 メゾブラスト・インターナショナル・エスアーエールエル 事前承認された組成物及び細胞培養方法
AU2023389700A1 (en) 2022-12-09 2025-07-10 Mesoblast International Sarl Method of treating heart failure in subjects with persistent inflammation
WO2024218715A1 (en) 2023-04-19 2024-10-24 Btl Healthcare Technologies A.S. Culture media based on protein hydrolysate and a process for preparing thereof
WO2024225602A1 (ko) * 2023-04-24 2024-10-31 파미셀 주식회사 뉴클레오시드 함유 세포 배양배지 및 이를 이용한 세포 배양 방법
KR20260061190A (ko) 2023-08-28 2026-05-06 메조블라스트 인터내셔널 에스에이알엘 GvHD에 대한 3차 치료
WO2025062365A1 (en) 2023-09-22 2025-03-27 Mesoblast International Sárl Mesenchymal lineage precursor or stem cells (mlpscs) for use in methods of treating congenital heart disease
US12668774B2 (en) 2023-10-12 2026-06-30 Btl Healthcare Technologies A.S. Product, system and method of cell cultivation

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US20250129337A1 (en) 2025-04-24
EP4008775A1 (en) 2022-06-08
CN113957043A (zh) 2022-01-21
JP2021090463A (ja) 2021-06-17
AU2016227607A1 (en) 2017-09-07
AU2021254538B2 (en) 2024-01-18
JP2018506986A (ja) 2018-03-15
JP6885870B2 (ja) 2021-06-16
KR20170120627A (ko) 2017-10-31
EP3265554B1 (en) 2021-11-17
BR112017018885A2 (pt) 2018-04-17
CN107567494A (zh) 2018-01-09
WO2016139340A1 (en) 2016-09-09
JP2024001179A (ja) 2024-01-09
EP3265554A1 (en) 2018-01-10
US11685899B2 (en) 2023-06-27
KR102635212B1 (ko) 2024-02-13
CA2977578A1 (en) 2016-09-09
US20240191200A1 (en) 2024-06-13
US20200040303A1 (en) 2020-02-06
AU2021254538A1 (en) 2021-11-18
JP7410899B2 (ja) 2024-01-10
US12215352B2 (en) 2025-02-04
SG11201706886PA (en) 2017-09-28
US20180037867A1 (en) 2018-02-08
CA2977578C (en) 2024-09-10

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