CN113957043A - 间充质干细胞的细胞培养方法 - Google Patents

间充质干细胞的细胞培养方法 Download PDF

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CN113957043A
CN113957043A CN202111251410.XA CN202111251410A CN113957043A CN 113957043 A CN113957043 A CN 113957043A CN 202111251410 A CN202111251410 A CN 202111251410A CN 113957043 A CN113957043 A CN 113957043A
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P·西蒙斯
C·叙尔
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Mesoblast International SARL
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
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    • C12N5/0663Bone marrow mesenchymal stem cells (BM-MSC)
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
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    • C12N2502/00Coculture with; Conditioned medium produced by
    • C12N2502/11Coculture with; Conditioned medium produced by blood or immune system cells
    • C12N2502/115Platelets, megakaryocytes

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CN202111251410.XA 2015-03-04 2016-03-04 间充质干细胞的细胞培养方法 Pending CN113957043A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
AU2015900752 2015-03-04
AU2015900752A AU2015900752A0 (en) 2015-03-04 Cell culture method
AU2015900777 2015-03-05
AU2015900777A AU2015900777A0 (en) 2015-03-05 Cell culture method
CN201680025469.8A CN107567494A (zh) 2015-03-04 2016-03-04 间充质干细胞的细胞培养方法

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Publications (1)

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CN113957043A true CN113957043A (zh) 2022-01-21

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CN201680025469.8A Pending CN107567494A (zh) 2015-03-04 2016-03-04 间充质干细胞的细胞培养方法
CN202111251410.XA Pending CN113957043A (zh) 2015-03-04 2016-03-04 间充质干细胞的细胞培养方法

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US (4) US20180037867A1 (enExample)
EP (2) EP4008775A1 (enExample)
JP (3) JP6885870B2 (enExample)
KR (1) KR102635212B1 (enExample)
CN (2) CN107567494A (enExample)
AU (2) AU2016227607B2 (enExample)
BR (1) BR112017018885A2 (enExample)
CA (1) CA2977578C (enExample)
SG (1) SG11201706886PA (enExample)
WO (1) WO2016139340A1 (enExample)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3095856T3 (da) 2008-09-16 2019-10-21 Mayo Found Medical Education & Res Sammensætninger med trombocytindhold
WO2012048099A2 (en) 2010-10-08 2012-04-12 Osiris Therapeutics, Inc. Nanoparticle-loaded cells
CA3138143A1 (en) 2014-05-16 2015-11-19 Mayo Foundation For Medical Education And Research Cell culture media compositions for primary cells
EP4008775A1 (en) 2015-03-04 2022-06-08 Mesoblast International Sàrl Cell culture method for mesenchymal stem cells
EP3714038A1 (en) 2017-11-22 2020-09-30 Mesoblast International Sàrl Cellular compositions and methods of treatment i
CN108251358B (zh) * 2017-12-15 2021-10-26 广东药科大学 一种相同供体来源人间充质干细胞的多批次原代分离方法
US20210115395A1 (en) * 2018-03-28 2021-04-22 Pluristem Ltd. Methods for expanding adherent stromal cells and cells obtained thereby
CN108624554B (zh) * 2018-05-14 2021-09-14 山东大学 一种非基因修饰光操控双向调节人脐带间充质干细胞增殖的方法
WO2020060809A1 (en) * 2018-09-17 2020-03-26 Rejuvablast LLC Combination grafts for tissue repair or regeneration applications
WO2020067443A1 (ja) * 2018-09-27 2020-04-02 テルモ株式会社 体細胞のシート化方法
CN115997005A (zh) * 2020-06-11 2023-04-21 迈索布拉斯特国际有限公司 间充质谱系前体或干细胞的3d培养
CN117529234A (zh) * 2021-03-10 2024-02-06 寺崎生物医学创新研究所 生产产品例如动物源性产品的方法和系统
EP4304383A4 (en) * 2021-03-10 2025-02-26 Terasaki Institute For Biomedical Innovation GROWTH FACTORS FOR LABORATORY-GROWN MEAT AND OTHER APPLICATIONS
WO2024121818A1 (en) 2022-12-09 2024-06-13 Mesoblast International Sarl Method of treating heart failure in subjects with persistent inflammation
CN120641115A (zh) 2022-12-09 2025-09-12 迈索布拉斯特国际有限公司 用细胞组合物治疗炎症的方法
KR20250134601A (ko) 2022-12-09 2025-09-11 메조블라스트 인터내셔널 에스에이알엘 조건화 배지 및 그 용도
JP2025538783A (ja) 2022-12-09 2025-11-28 メゾブラスト・インターナショナル・エスアーエールエル 事前承認された組成物及び細胞培養方法
WO2024225602A1 (ko) * 2023-04-24 2024-10-31 파미셀 주식회사 뉴클레오시드 함유 세포 배양배지 및 이를 이용한 세포 배양 방법
WO2025046491A1 (en) 2023-08-28 2025-03-06 Mesoblast International Sàrl Third line treatment for gvhd
WO2025062365A1 (en) 2023-09-22 2025-03-27 Mesoblast International Sárl Mesenchymal lineage precursor or stem cells (mlpscs) for use in methods of treating congenital heart disease

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101072868A (zh) * 2004-09-28 2007-11-14 威塞尔研究所股份有限公司 灵长类胚胎干细胞的培养
US20090232777A1 (en) * 2006-03-01 2009-09-17 Evy Lundgren-Akerlund Expansion and Differentiation of Mesenchymal Stem Cells
CN102791276A (zh) * 2010-03-10 2012-11-21 智再如股份有限公司 含有间充质干细胞的细胞制品及其制造方法

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5486359A (en) 1990-11-16 1996-01-23 Osiris Therapeutics, Inc. Human mesenchymal stem cells
EP0978259B1 (de) 1991-08-12 2002-02-20 Karl Storz GmbH & Co. KG Hochfrequenzchirurgiegenerator mit einer einstellbaren Ausgangsleistung
US5814464A (en) 1994-10-07 1998-09-29 Regeneron Pharma Nucleic acids encoding TIE-2 ligand-2
US6277372B1 (en) 1994-11-08 2001-08-21 Diacrin, Inc. Porcine neural cells and their use in treatment of neurological deficits due to neurodegenerative diseases
US6204053B1 (en) 1994-11-08 2001-03-20 Diacrin, Inc. Porcine cortical cells and their use in treatment of neurological deficits due to neurodegenerative diseases
ATE273384T1 (de) 1995-04-06 2004-08-15 Regeneron Pharma Tie-2 liganden, herstellungsverfahren und verwendung
AU5734998A (en) 1997-01-10 1998-08-03 Life Technologies, Inc. Embryonic stem cell serum replacement
US5968829A (en) 1997-09-05 1999-10-19 Cytotherapeutics, Inc. Human CNS neural stem cells
RU2272839C2 (ru) * 1998-11-09 2006-03-27 Консорцио Пер Ла Джестионе Дель Чентро Ди Биотекнолоджие Аванцате Бессывороточная среда для культуры клеток, используемых для реконструкции костных и хрящевых сегментов (варианты)
AU2003901668A0 (en) 2003-03-28 2003-05-01 Medvet Science Pty. Ltd. Non-haemopoietic precursor cells
WO2001028574A2 (en) 1999-10-20 2001-04-26 Stem Cells, Inc. Methods for inducing in vivo proliferation and migration of transplanted progenitor cells in the brain
JP2004528021A (ja) 2001-02-14 2004-09-16 アンスロジェネシス コーポレーション 分娩後の哺乳動物の胎盤、その使用およびそれに由来する胎盤幹細胞
ES2545899T3 (es) 2001-02-14 2015-09-16 Anthrogenesis Corporation Placenta post-parto de mamíferos, su uso y células madre placentarias de la misma
JP2005500847A (ja) 2001-08-24 2005-01-13 アドバンスド セル テクノロジー、インコーポレイテッド 分化誘導薬の同定のためのスクリーニング検定及び細胞治療用の分化細胞の調製
MXPA04007732A (es) 2002-02-13 2004-10-15 Anthrogenesis Corp Celulas madre similares a las embrionarias, derivadas de la placenta de mamiferos despues del parto y usos y metodos de tratamiento usando dichas celulas.
WO2003102134A2 (en) 2002-05-28 2003-12-11 Becton, Dickinson And Company Pancreatic acinar cells into insulin producing cells
EP1507552A2 (en) 2002-05-28 2005-02-23 Novocell, Inc. Methods, compositions, and growth and differentiation factors for insulin-producing cells
EP1641915B1 (en) 2003-06-27 2016-07-27 DePuy Synthes Products, Inc. Repair and regeneration of ocular tissue using postpartum-derived cells
US8491883B2 (en) 2003-06-27 2013-07-23 Advanced Technologies And Regenerative Medicine, Llc Treatment of amyotrophic lateral sclerosis using umbilical derived cells
EP1758984A2 (en) 2004-02-26 2007-03-07 Reliance Life Sciences Pvt., Ltd. Pluripotent embryonic-like stem cells derived from corneal limbus, methods of isolation and uses thereof
ES2621847T3 (es) 2004-12-23 2017-07-05 DePuy Synthes Products, Inc. Células posparto derivadas de tejido de cordón umbilical, y métodos de elaboración y uso de las mismas
EP1833496B1 (en) 2004-12-23 2013-07-31 Ethicon, Incorporated Treatment of stroke and other acute neural degenerative disorders using postpartum derived cells
WO2006071773A2 (en) 2004-12-23 2006-07-06 Ethicon Incoporated Treatment of osteochondral diseases using postpartum-derived cells and products thereof
WO2006071777A2 (en) 2004-12-23 2006-07-06 Ethicon Incorporated Soft tissue repair and regeneration using postpartum-derived cells and cell products
US20080199849A1 (en) 2005-09-02 2008-08-21 Agency For Science, Technology And Research Method of Deriving Progenitor Cell Line
KR101107835B1 (ko) 2006-01-13 2012-02-09 가부시키가이샤 투셀 동물 세포의 무혈청 배양에 사용하기 위한 배양 배지 첨가물, 키트 및 이의 용도
JP2010500047A (ja) * 2006-08-15 2010-01-07 エージェンシー フォー サイエンス,テクノロジー アンド リサーチ 間葉系幹細胞馴化培地
JP2008148643A (ja) * 2006-12-19 2008-07-03 Olympus Corp 無血清培地および幹細胞の培養方法
RS52921B (sr) 2007-02-12 2014-02-28 Anthrogenesis Corporation Lečenje inflamatornih bolesti upotrebom placentnih matičnih ćelija
WO2008151390A1 (en) 2007-06-15 2008-12-18 Australian Stem Cell Centre Ltd Differentiation of human embryonic stem cells
US20110236971A2 (en) * 2007-09-25 2011-09-29 Maksym Vodyanyk Generation of Clonal Mesenchymal Progenitors and Mesenchymal Stem Cell Lines Under Serum-Free Conditions
KR101224588B1 (ko) * 2008-01-24 2013-01-22 삼성전자주식회사 멀티포인트 스트록을 감지하기 위한 ui 제공방법 및 이를적용한 멀티미디어 기기
US20110110888A1 (en) 2008-06-27 2011-05-12 Hai-Qing Xian Scaffold Coated and/or Impregnated with at Least One Bioactive Agent for Tissue Repair and Other Medical Applications
US20100055078A1 (en) * 2008-08-15 2010-03-04 The Government of the U.S.A, as represented by the Department of Veterans Affairs Methods of making a transplantable bone repair system using multipotent stem cells
US20110212523A1 (en) 2008-11-11 2011-09-01 Yukio Kato Differentiation-inducing culture medium additive and use thereof
US20100158878A1 (en) 2008-12-23 2010-06-24 Alexandra Capela Target populations of oligodendrocyte precursor cells and methods of making and using same
US10894944B2 (en) 2009-04-10 2021-01-19 Monash University Cell culture media
AU2011265723A1 (en) * 2010-06-17 2013-01-10 Stemrd, Inc. Serum-free chemically defined cell culture medium
CA2821065C (en) * 2011-01-12 2021-05-18 Tsuneo KIDO Culture method to obtain and maintain a pure or enriched population of mammalian neural stem cells and/or neural progenitor cells that are prone to differentiate into oligodendrocyte-lineage cells in vitro
KR101523040B1 (ko) * 2012-06-26 2015-05-26 라정찬 고농도의 줄기세포 제조방법
WO2014082685A1 (en) * 2012-11-30 2014-06-05 Swiss Stem Cell Foundation Serum-free medium for human mesenchymal stem cells
CN103396984B (zh) * 2013-07-25 2016-02-10 宗兆文 一种利用皮肤来源的祖细胞制备组织工程脊髓的方法
EP4008775A1 (en) 2015-03-04 2022-06-08 Mesoblast International Sàrl Cell culture method for mesenchymal stem cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101072868A (zh) * 2004-09-28 2007-11-14 威塞尔研究所股份有限公司 灵长类胚胎干细胞的培养
US20090232777A1 (en) * 2006-03-01 2009-09-17 Evy Lundgren-Akerlund Expansion and Differentiation of Mesenchymal Stem Cells
CN102791276A (zh) * 2010-03-10 2012-11-21 智再如股份有限公司 含有间充质干细胞的细胞制品及其制造方法

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WO2016139340A1 (en) 2016-09-09
US11685899B2 (en) 2023-06-27
US20200040303A1 (en) 2020-02-06
US20180037867A1 (en) 2018-02-08
EP3265554A1 (en) 2018-01-10
SG11201706886PA (en) 2017-09-28
JP7410899B2 (ja) 2024-01-10
AU2016227607A1 (en) 2017-09-07
US20250129337A1 (en) 2025-04-24
JP2021090463A (ja) 2021-06-17
AU2021254538B2 (en) 2024-01-18
EP4008775A1 (en) 2022-06-08
US20240191200A1 (en) 2024-06-13
CA2977578A1 (en) 2016-09-09
CA2977578C (en) 2024-09-10
AU2021254538A1 (en) 2021-11-18
JP2018506986A (ja) 2018-03-15
US12215352B2 (en) 2025-02-04
EP3265554B1 (en) 2021-11-17
KR102635212B1 (ko) 2024-02-13
KR20170120627A (ko) 2017-10-31
JP2024001179A (ja) 2024-01-09
JP6885870B2 (ja) 2021-06-16
BR112017018885A2 (pt) 2018-04-17
AU2016227607B2 (en) 2021-09-30
CN107567494A (zh) 2018-01-09

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