BR112017018885A2 - método de cultura celular para células-tronco mesenquimais - Google Patents
método de cultura celular para células-tronco mesenquimaisInfo
- Publication number
- BR112017018885A2 BR112017018885A2 BR112017018885A BR112017018885A BR112017018885A2 BR 112017018885 A2 BR112017018885 A2 BR 112017018885A2 BR 112017018885 A BR112017018885 A BR 112017018885A BR 112017018885 A BR112017018885 A BR 112017018885A BR 112017018885 A2 BR112017018885 A2 BR 112017018885A2
- Authority
- BR
- Brazil
- Prior art keywords
- cell culture
- stem cells
- mesenchymal stem
- culture method
- compositions
- Prior art date
Links
- 238000004113 cell culture Methods 0.000 title abstract 2
- 210000002901 mesenchymal stem cell Anatomy 0.000 title 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 abstract 1
- 239000006143 cell culture medium Substances 0.000 abstract 1
- 230000004663 cell proliferation Effects 0.000 abstract 1
- 239000012091 fetal bovine serum Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/99—Serum-free medium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/135—Platelet-derived growth factor [PDGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
- C12N2502/115—Platelets, megakaryocytes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Rheumatology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2015900752A AU2015900752A0 (en) | 2015-03-04 | Cell culture method | |
| AU2015900777A AU2015900777A0 (en) | 2015-03-05 | Cell culture method | |
| PCT/EP2016/054640 WO2016139340A1 (en) | 2015-03-04 | 2016-03-04 | Cell culture method for mesenchymal stem cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112017018885A2 true BR112017018885A2 (pt) | 2018-04-17 |
Family
ID=55453191
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112017018885A BR112017018885A2 (pt) | 2015-03-04 | 2016-03-04 | método de cultura celular para células-tronco mesenquimais |
Country Status (10)
| Country | Link |
|---|---|
| US (4) | US20180037867A1 (enExample) |
| EP (2) | EP3265554B1 (enExample) |
| JP (3) | JP6885870B2 (enExample) |
| KR (1) | KR102635212B1 (enExample) |
| CN (2) | CN113957043A (enExample) |
| AU (2) | AU2016227607B2 (enExample) |
| BR (1) | BR112017018885A2 (enExample) |
| CA (1) | CA2977578C (enExample) |
| SG (1) | SG11201706886PA (enExample) |
| WO (1) | WO2016139340A1 (enExample) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2334785B2 (en) * | 2008-09-16 | 2019-08-14 | Mayo Foundation For Medical Education And Research | Compositions containing platelet contents |
| US20120087868A1 (en) | 2010-10-08 | 2012-04-12 | Gabriele Todd | Nanoparticle-loaded cells |
| EP4328299A3 (en) | 2014-05-16 | 2024-05-29 | Mayo Foundation for Medical Education and Research | Cell culture media compositions for primary cells |
| US20180037867A1 (en) | 2015-03-04 | 2018-02-08 | Mesoblast International Sàrl | Cell culture method for mesenchymal stem cells |
| CN112004923A (zh) | 2017-11-22 | 2020-11-27 | 迈索布拉斯特国际有限公司 | 细胞组合物及治疗方法 |
| CN108251358B (zh) * | 2017-12-15 | 2021-10-26 | 广东药科大学 | 一种相同供体来源人间充质干细胞的多批次原代分离方法 |
| US20210115395A1 (en) * | 2018-03-28 | 2021-04-22 | Pluristem Ltd. | Methods for expanding adherent stromal cells and cells obtained thereby |
| CN108624554B (zh) * | 2018-05-14 | 2021-09-14 | 山东大学 | 一种非基因修饰光操控双向调节人脐带间充质干细胞增殖的方法 |
| WO2020060809A1 (en) * | 2018-09-17 | 2020-03-26 | Rejuvablast LLC | Combination grafts for tissue repair or regeneration applications |
| WO2020067443A1 (ja) * | 2018-09-27 | 2020-04-02 | テルモ株式会社 | 体細胞のシート化方法 |
| CA3181611A1 (en) * | 2020-06-11 | 2021-12-16 | Paul Simmons | 3d culture of mesenchymal lineage precursor or stem cells |
| CN117396077A (zh) * | 2021-03-10 | 2024-01-12 | 寺崎生物医学创新研究所 | 用于实验室生长肉和其他应用的生长因子 |
| EP4304383A4 (en) * | 2021-03-10 | 2025-02-26 | Terasaki Institute For Biomedical Innovation | GROWTH FACTORS FOR LABORATORY-GROWN MEAT AND OTHER APPLICATIONS |
| EP4630536A1 (en) | 2022-12-09 | 2025-10-15 | Mesoblast International Sàrl | Conditioned media and use of the same |
| JP2025538783A (ja) | 2022-12-09 | 2025-11-28 | メゾブラスト・インターナショナル・エスアーエールエル | 事前承認された組成物及び細胞培養方法 |
| CN120513094A (zh) | 2022-12-09 | 2025-08-19 | 迈索布拉斯特国际有限公司 | 治疗患有持续性炎症的受试者的心力衰竭的方法 |
| WO2024121819A1 (en) | 2022-12-09 | 2024-06-13 | Mesoblast International Sarl | Method of treating inflammation with cellular compositions |
| WO2024225602A1 (ko) * | 2023-04-24 | 2024-10-31 | 파미셀 주식회사 | 뉴클레오시드 함유 세포 배양배지 및 이를 이용한 세포 배양 방법 |
| WO2025046491A1 (en) | 2023-08-28 | 2025-03-06 | Mesoblast International Sàrl | Third line treatment for gvhd |
| WO2025062365A1 (en) | 2023-09-22 | 2025-03-27 | Mesoblast International Sárl | Mesenchymal lineage precursor or stem cells (mlpscs) for use in methods of treating congenital heart disease |
Family Cites Families (46)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5486359A (en) | 1990-11-16 | 1996-01-23 | Osiris Therapeutics, Inc. | Human mesenchymal stem cells |
| DE59209946D1 (de) | 1991-08-12 | 2002-03-28 | Storz Karl Gmbh & Co Kg | Hochfrequenzchirurgiegenerator mit einer einstellbaren Ausgangsleistung |
| US5814464A (en) | 1994-10-07 | 1998-09-29 | Regeneron Pharma | Nucleic acids encoding TIE-2 ligand-2 |
| US6204053B1 (en) | 1994-11-08 | 2001-03-20 | Diacrin, Inc. | Porcine cortical cells and their use in treatment of neurological deficits due to neurodegenerative diseases |
| US6277372B1 (en) | 1994-11-08 | 2001-08-21 | Diacrin, Inc. | Porcine neural cells and their use in treatment of neurological deficits due to neurodegenerative diseases |
| EP0821728B1 (en) | 1995-04-06 | 2004-08-11 | Regeneron Pharmaceuticals, Inc. | Tie-2 ligands, methods of making and uses thereof |
| EP0986635A4 (en) | 1997-01-10 | 2001-11-07 | Life Technologies Inc | SERUM SUBSTITUTE FOR EMBRYONIC STEM CELLS |
| US5968829A (en) | 1997-09-05 | 1999-10-19 | Cytotherapeutics, Inc. | Human CNS neural stem cells |
| CA2348687A1 (en) * | 1998-11-09 | 2000-05-18 | Consorzio Per La Gestione Del Centro Di Biotecnologie Avanzate | Serum free medium for chondrocyte-like cells |
| AU2003901668A0 (en) | 2003-03-28 | 2003-05-01 | Medvet Science Pty. Ltd. | Non-haemopoietic precursor cells |
| AU2118501A (en) | 1999-10-20 | 2001-04-30 | Stemcells, Inc. | Methods for inducing in vivo proliferation and migration of transplanted progenitor cells in the brain |
| US7439064B2 (en) | 2000-03-09 | 2008-10-21 | Wicell Research Institute, Inc. | Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium |
| NZ527849A (en) | 2001-02-14 | 2006-09-29 | Anthrogenesis Corp | Post-partum mammalian placenta, its use and placental stem cells therefrom |
| NZ528035A (en) | 2001-02-14 | 2005-07-29 | Robert J Hariri | Renovation and repopulation of decellularized tissues and cadaveric organs by stem cells |
| IL160507A0 (en) | 2001-08-24 | 2004-07-25 | Advanced Cell Tech Inc | Screening assays for identifying differentiation-inducing agents and production of differentiated cells for cell therapy |
| JP2005517402A (ja) | 2002-02-13 | 2005-06-16 | アンスロジェネシス コーポレーション | 分娩後の哺乳動物胎盤由来の胚様幹細胞、ならびに該細胞の用途および該細胞を用いる治療法 |
| CN1662643A (zh) | 2002-05-28 | 2005-08-31 | 贝克顿·迪金森公司 | 人腺泡细胞的扩增和转分化 |
| CN1668324A (zh) | 2002-05-28 | 2005-09-14 | 诺沃塞尔公司 | 用于胰岛素产生细胞的方法、组合物及生长与分化因子 |
| US8491883B2 (en) | 2003-06-27 | 2013-07-23 | Advanced Technologies And Regenerative Medicine, Llc | Treatment of amyotrophic lateral sclerosis using umbilical derived cells |
| CA2530421C (en) | 2003-06-27 | 2015-04-21 | Ethicon, Incorporated | Repair and regeneration of ocular tissue using postpartum-derived cells |
| KR100931887B1 (ko) | 2004-02-26 | 2009-12-15 | 리라이언스 라이프 사이언시스 프라이빗. 리미티드 | 각막 윤부에서 유도된 다능성 배아 유사 줄기 세포, 분리 방법 및 이들의 용도 |
| WO2006071777A2 (en) | 2004-12-23 | 2006-07-06 | Ethicon Incorporated | Soft tissue repair and regeneration using postpartum-derived cells and cell products |
| JP5340599B2 (ja) | 2004-12-23 | 2013-11-13 | エシコン・インコーポレイテッド | 臍帯組織由来産褥細胞ならびにその製造方法および使用方法 |
| WO2006071773A2 (en) | 2004-12-23 | 2006-07-06 | Ethicon Incoporated | Treatment of osteochondral diseases using postpartum-derived cells and products thereof |
| EP1835924B1 (en) | 2004-12-23 | 2013-08-21 | Ethicon, Incorporated | Treatment of parkinson's disease and related disorders using postpartum derived cells |
| BRPI0617084A2 (pt) | 2005-09-02 | 2011-07-12 | Agency Science Tech & Res | método, linhagem de célula progenitora, célula diferenciada e método para gerar uma célula diferenciada de uma célula-tronco (es) embrionária |
| US9074176B2 (en) | 2006-01-13 | 2015-07-07 | Two Cells Co., Ltd. | Culture medium additive for use in serum-free culturing of animal cell, kit and use thereof |
| WO2007099337A1 (en) * | 2006-03-01 | 2007-09-07 | Cartela R&D Ab | Expansion and differentiation of mesenchymal stem cells |
| EP2054506A1 (en) * | 2006-08-15 | 2009-05-06 | Agency for Science, Technology and Research | Mesenchymal stem cell conditioned medium |
| JP2008148643A (ja) * | 2006-12-19 | 2008-07-03 | Olympus Corp | 無血清培地および幹細胞の培養方法 |
| HRP20130765T1 (hr) | 2007-02-12 | 2013-10-25 | Anthrogenesis Corporation | Lijeäśenje protuupalnih bolesti putem matiäśnih stanica posteljice |
| WO2008151390A1 (en) | 2007-06-15 | 2008-12-18 | Australian Stem Cell Centre Ltd | Differentiation of human embryonic stem cells |
| US20110236971A2 (en) * | 2007-09-25 | 2011-09-29 | Maksym Vodyanyk | Generation of Clonal Mesenchymal Progenitors and Mesenchymal Stem Cell Lines Under Serum-Free Conditions |
| KR101224588B1 (ko) * | 2008-01-24 | 2013-01-22 | 삼성전자주식회사 | 멀티포인트 스트록을 감지하기 위한 ｕｉ 제공방법 및 이를적용한 멀티미디어 기기 |
| WO2009158634A2 (en) | 2008-06-27 | 2009-12-30 | Zimmer Orthobiologics, Inc. | Scaffold coated and/or impregnated with at least one bioactive agent for tissue repair and other medical applications |
| US20100055078A1 (en) * | 2008-08-15 | 2010-03-04 | The Government of the U.S.A, as represented by the Department of Veterans Affairs | Methods of making a transplantable bone repair system using multipotent stem cells |
| JP5660572B2 (ja) | 2008-11-11 | 2015-01-28 | 国立大学法人広島大学 | 分化誘導培地用添加剤およびその利用 |
| US20100158878A1 (en) | 2008-12-23 | 2010-06-24 | Alexandra Capela | Target populations of oligodendrocyte precursor cells and methods of making and using same |
| US10894944B2 (en) | 2009-04-10 | 2021-01-19 | Monash University | Cell culture media |
| JP5804385B2 (ja) * | 2010-03-10 | 2015-11-04 | 株式会社ツーセル | 間葉系幹細胞を含む細胞製剤及びその製造方法 |
| CN103068969A (zh) * | 2010-06-17 | 2013-04-24 | 思迪公司 | 化学成分确定的无血清细胞培养基 |
| MX364742B (es) * | 2011-01-12 | 2019-05-07 | Tsuneo Kido | Metodo de cultivo para obtener y mantener una poblacion pura o enriquecida de celulas no diferenciadas neurales y/o celulas progenitoras neurales de mamifero que con propensas a diferenciarse en celulas del linaje oligodendrocitico in vitro. |
| KR101523040B1 (ko) * | 2012-06-26 | 2015-05-26 | 라정찬 | 고농도의 줄기세포 제조방법 |
| WO2014082685A1 (en) * | 2012-11-30 | 2014-06-05 | Swiss Stem Cell Foundation | Serum-free medium for human mesenchymal stem cells |
| CN103396984B (zh) * | 2013-07-25 | 2016-02-10 | 宗兆文 | 一种利用皮肤来源的祖细胞制备组织工程脊髓的方法 |
| US20180037867A1 (en) | 2015-03-04 | 2018-02-08 | Mesoblast International Sàrl | Cell culture method for mesenchymal stem cells |
-
2016
- 2016-03-04 US US15/553,115 patent/US20180037867A1/en not_active Abandoned
- 2016-03-04 SG SG11201706886PA patent/SG11201706886PA/en unknown
- 2016-03-04 AU AU2016227607A patent/AU2016227607B2/en active Active
- 2016-03-04 BR BR112017018885A patent/BR112017018885A2/pt not_active Application Discontinuation
- 2016-03-04 WO PCT/EP2016/054640 patent/WO2016139340A1/en not_active Ceased
- 2016-03-04 EP EP16707810.4A patent/EP3265554B1/en active Active
- 2016-03-04 KR KR1020177025840A patent/KR102635212B1/ko active Active
- 2016-03-04 JP JP2017546094A patent/JP6885870B2/ja active Active
- 2016-03-04 CN CN202111251410.XA patent/CN113957043A/zh active Pending
- 2016-03-04 CA CA2977578A patent/CA2977578C/en active Active
- 2016-03-04 EP EP21205011.6A patent/EP4008775A1/en active Pending
- 2016-03-04 CN CN201680025469.8A patent/CN107567494A/zh active Pending
-
2019
- 2019-10-11 US US16/599,271 patent/US11685899B2/en active Active
-
2021
- 2021-03-16 JP JP2021042409A patent/JP7410899B2/ja active Active
- 2021-10-19 AU AU2021254538A patent/AU2021254538B2/en active Active
-
2023
- 2023-03-21 US US18/124,346 patent/US12215352B2/en active Active
- 2023-10-05 JP JP2023173585A patent/JP2024001179A/ja active Pending
-
2024
- 2024-12-24 US US19/001,125 patent/US20250129337A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CA2977578A1 (en) | 2016-09-09 |
| EP3265554B1 (en) | 2021-11-17 |
| KR20170120627A (ko) | 2017-10-31 |
| US20240191200A1 (en) | 2024-06-13 |
| EP3265554A1 (en) | 2018-01-10 |
| JP7410899B2 (ja) | 2024-01-10 |
| AU2021254538A1 (en) | 2021-11-18 |
| SG11201706886PA (en) | 2017-09-28 |
| AU2016227607A1 (en) | 2017-09-07 |
| US20250129337A1 (en) | 2025-04-24 |
| JP2021090463A (ja) | 2021-06-17 |
| US20200040303A1 (en) | 2020-02-06 |
| US12215352B2 (en) | 2025-02-04 |
| US11685899B2 (en) | 2023-06-27 |
| JP2024001179A (ja) | 2024-01-09 |
| JP2018506986A (ja) | 2018-03-15 |
| AU2016227607B2 (en) | 2021-09-30 |
| AU2021254538B2 (en) | 2024-01-18 |
| WO2016139340A1 (en) | 2016-09-09 |
| JP6885870B2 (ja) | 2021-06-16 |
| CN113957043A (zh) | 2022-01-21 |
| CN107567494A (zh) | 2018-01-09 |
| CA2977578C (en) | 2024-09-10 |
| EP4008775A1 (en) | 2022-06-08 |
| US20180037867A1 (en) | 2018-02-08 |
| KR102635212B1 (ko) | 2024-02-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| BR112017018885A2 (pt) | método de cultura celular para células-tronco mesenquimais | |
| ZA202310779B (en) | Compositions and methods for enhanced gene expression | |
| GB2548740A (en) | Methods for generation of podocytes from pluripotent stem cells and cells produced by the same | |
| AU2016250905A8 (en) | Generation of muscle-lineage cells from stem cells | |
| MX2021000459A (es) | Metodo para producir celulas t gamma y delta. | |
| MX2016014235A (es) | Biosintesis microbiana de ergotioneina. | |
| EP3161127A4 (en) | Cultured mammalian limbal stem cells, methods for generating the same, and uses thereof | |
| MX2017007196A (es) | Fibroblastos de embrion de pollo inmortalizados. | |
| CA2981708C (en) | EX VIVO PROLIFERATION OF EPITHELIAL CELLS | |
| TW201612312A (en) | Meristematic plant cells and method of isolating them | |
| MX2020009670A (es) | Metodos de cultivo por perfusion y usos de los mismos. | |
| MX2018014043A (es) | Composiciones de cultivo celular con antioxidantes y metodos para la produccion de polipeptidos. | |
| EP3263697A4 (en) | Culture medium for culturing mesenchymal stem cell, method for culturing mesenchymal stem cell, and mesenchymal stem cell | |
| TW201612308A (en) | Collector architecture layout design | |
| MX2015012168A (es) | Expansión de celulas madre adultas in vitro. | |
| MY197129A (en) | Electrochemical coupling of two phenols which differ in their oxidation potential | |
| MX366112B (es) | Medio de cultivo celular y metodos de produccion de anticuerpos. | |
| EP3202896A4 (en) | Method for culturing pluripotent stem cells | |
| HK1254740A1 (zh) | 分化和纯化胰腺内分泌细胞的方法 | |
| EP3346002A4 (en) | Method for reducing pluripotent stem cells, method for producing cell population having reduced pluripotent stem cells | |
| EP3378935A4 (en) | Bovine serum composition and method for culturing cells using said bovine serum composition as additive | |
| BR112017020513A2 (pt) | método de produção de peixe para transplante, peixe para transplante, método de produção de espécie híbrida de peixe e espécie híbrida de peixe | |
| PL3436568T3 (pl) | Podłoże hodowlane do pluripotencjalnych komórek macierzystych | |
| EA201791233A1 (ru) | Материал стволовых клеток и способ его получения | |
| WO2015171852A3 (en) | Methods and compositions for non-cytotoxic stem cell transplantation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
| B09B | Patent application refused [chapter 9.2 patent gazette] | ||
| B154 | Notification of filing of divisional application [chapter 15.50 patent gazette] |
Free format text: O PEDIDO FOI DIVIDIDO NO BR122024005049-8 PROTOCOLO 870240021808 EM 14/03/2024 14:37. |
|
| B12B | Appeal against refusal [chapter 12.2 patent gazette] |