CN107567494A - 间充质干细胞的细胞培养方法 - Google Patents

间充质干细胞的细胞培养方法 Download PDF

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Publication number
CN107567494A
CN107567494A CN201680025469.8A CN201680025469A CN107567494A CN 107567494 A CN107567494 A CN 107567494A CN 201680025469 A CN201680025469 A CN 201680025469A CN 107567494 A CN107567494 A CN 107567494A
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culture medium
pdgf
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P·西蒙斯
C·叙尔
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Mesoblast International SARL
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/135—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
    • C12N2502/115—Platelets, megakaryocytes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CN201680025469.8A 2015-03-04 2016-03-04 间充质干细胞的细胞培养方法 Pending CN107567494A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111251410.XA CN113957043A (zh) 2015-03-04 2016-03-04 间充质干细胞的细胞培养方法

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
AU2015900752A AU2015900752A0 (en) 2015-03-04 Cell culture method
AU2015900752 2015-03-04
AU2015900777 2015-03-05
AU2015900777A AU2015900777A0 (en) 2015-03-05 Cell culture method
PCT/EP2016/054640 WO2016139340A1 (en) 2015-03-04 2016-03-04 Cell culture method for mesenchymal stem cells

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CN201680025469.8A Pending CN107567494A (zh) 2015-03-04 2016-03-04 间充质干细胞的细胞培养方法

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US (4) US20180037867A1 (enExample)
EP (2) EP4008775A1 (enExample)
JP (3) JP6885870B2 (enExample)
KR (1) KR102635212B1 (enExample)
CN (2) CN113957043A (enExample)
AU (2) AU2016227607B2 (enExample)
BR (1) BR112017018885A2 (enExample)
CA (1) CA2977578C (enExample)
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Cited By (2)

* Cited by examiner, † Cited by third party
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CN108624554A (zh) * 2018-05-14 2018-10-09 山东大学 一种非基因修饰光操控双向调节人脐带间充质干细胞增殖的方法
CN115997005A (zh) * 2020-06-11 2023-04-21 迈索布拉斯特国际有限公司 间充质谱系前体或干细胞的3d培养

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EP3143130B1 (en) 2014-05-16 2024-03-06 Mayo Foundation for Medical Education and Research Cell culture media compositions for primary cells
EP4008775A1 (en) 2015-03-04 2022-06-08 Mesoblast International Sàrl Cell culture method for mesenchymal stem cells
WO2019102268A1 (en) 2017-11-22 2019-05-31 Mesoblast International Sarl Cellular compositions and methods of treatment i
CN108251358B (zh) * 2017-12-15 2021-10-26 广东药科大学 一种相同供体来源人间充质干细胞的多批次原代分离方法
SG11202009393QA (en) * 2018-03-28 2020-10-29 Pluristem Ltd Methods for expanding adherent stromal cells and cells obtained thereby
US11364323B2 (en) 2018-09-17 2022-06-21 Rejuvablast LLC Combination grafts for tissue repair or regeneration applications
JPWO2020067443A1 (ja) * 2018-09-27 2021-08-30 テルモ株式会社 体細胞のシート化方法
US20240150724A1 (en) * 2021-03-10 2024-05-09 Terasaki Institute For Biomedical Innovation Production of heme for cell-based meat products
CN117529234A (zh) * 2021-03-10 2024-02-06 寺崎生物医学创新研究所 生产产品例如动物源性产品的方法和系统
AU2023389703A1 (en) 2022-12-09 2025-07-10 Mesoblast International Sarl Conditioned media and use of the same
AU2023389700A1 (en) 2022-12-09 2025-07-10 Mesoblast International Sarl Method of treating heart failure in subjects with persistent inflammation
CN120603931A (zh) 2022-12-09 2025-09-05 迈索布拉斯特国际有限公司 预先许可的组合物和细胞培养方法
CN120641115A (zh) 2022-12-09 2025-09-12 迈索布拉斯特国际有限公司 用细胞组合物治疗炎症的方法
WO2024225602A1 (ko) * 2023-04-24 2024-10-31 파미셀 주식회사 뉴클레오시드 함유 세포 배양배지 및 이를 이용한 세포 배양 방법
WO2025046491A1 (en) 2023-08-28 2025-03-06 Mesoblast International Sàrl Third line treatment for gvhd
WO2025062365A1 (en) 2023-09-22 2025-03-27 Mesoblast International Sárl Mesenchymal lineage precursor or stem cells (mlpscs) for use in methods of treating congenital heart disease

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108624554A (zh) * 2018-05-14 2018-10-09 山东大学 一种非基因修饰光操控双向调节人脐带间充质干细胞增殖的方法
CN108624554B (zh) * 2018-05-14 2021-09-14 山东大学 一种非基因修饰光操控双向调节人脐带间充质干细胞增殖的方法
CN115997005A (zh) * 2020-06-11 2023-04-21 迈索布拉斯特国际有限公司 间充质谱系前体或干细胞的3d培养

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JP7410899B2 (ja) 2024-01-10
AU2016227607B2 (en) 2021-09-30
AU2016227607A1 (en) 2017-09-07
US20240191200A1 (en) 2024-06-13
US20250129337A1 (en) 2025-04-24
KR20170120627A (ko) 2017-10-31
AU2021254538A1 (en) 2021-11-18
SG11201706886PA (en) 2017-09-28
WO2016139340A1 (en) 2016-09-09
JP6885870B2 (ja) 2021-06-16
BR112017018885A2 (pt) 2018-04-17
CA2977578A1 (en) 2016-09-09
EP3265554B1 (en) 2021-11-17
JP2024001179A (ja) 2024-01-09
CN113957043A (zh) 2022-01-21
CA2977578C (en) 2024-09-10
KR102635212B1 (ko) 2024-02-13
JP2018506986A (ja) 2018-03-15
US20180037867A1 (en) 2018-02-08
EP3265554A1 (en) 2018-01-10
JP2021090463A (ja) 2021-06-17
EP4008775A1 (en) 2022-06-08
US20200040303A1 (en) 2020-02-06
US11685899B2 (en) 2023-06-27
US12215352B2 (en) 2025-02-04
AU2021254538B2 (en) 2024-01-18

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