CA2925659C - Method of producing l-amino acids - Google Patents
Method of producing l-amino acids Download PDFInfo
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- CA2925659C CA2925659C CA2925659A CA2925659A CA2925659C CA 2925659 C CA2925659 C CA 2925659C CA 2925659 A CA2925659 A CA 2925659A CA 2925659 A CA2925659 A CA 2925659A CA 2925659 C CA2925659 C CA 2925659C
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- gene
- acetate
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- amino acids
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- 238000004519 manufacturing process Methods 0.000 claims description 36
- 239000002773 nucleotide Substances 0.000 claims description 35
- 125000003729 nucleotide group Chemical group 0.000 claims description 35
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- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 5
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- 125000000539 amino acid group Chemical group 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000001099 ammonium carbonate Substances 0.000 description 1
- 235000012501 ammonium carbonate Nutrition 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 239000000908 ammonium hydroxide Substances 0.000 description 1
- 229910000148 ammonium phosphate Inorganic materials 0.000 description 1
- 235000019289 ammonium phosphates Nutrition 0.000 description 1
- 150000007514 bases Chemical class 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000004359 castor oil Substances 0.000 description 1
- 235000019438 castor oil Nutrition 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000003240 coconut oil Substances 0.000 description 1
- 235000019864 coconut oil Nutrition 0.000 description 1
- 108091036078 conserved sequence Proteins 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- 235000013681 dietary sucrose Nutrition 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 1
- 230000037341 glyoxalate cycle Effects 0.000 description 1
- HHLFWLYXYJOTON-UHFFFAOYSA-N glyoxylic acid Chemical compound OC(=O)C=O HHLFWLYXYJOTON-UHFFFAOYSA-N 0.000 description 1
- BHEPBYXIRTUNPN-UHFFFAOYSA-N hydridophosphorus(.) (triplet) Chemical compound [PH] BHEPBYXIRTUNPN-UHFFFAOYSA-N 0.000 description 1
- 229910000358 iron sulfate Inorganic materials 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 101150035025 lysC gene Proteins 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 238000012269 metabolic engineering Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- KHPXUQMNIQBQEV-UHFFFAOYSA-N oxaloacetic acid Chemical compound OC(=O)CC(=O)C(O)=O KHPXUQMNIQBQEV-UHFFFAOYSA-N 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 229920001522 polyglycol ester Polymers 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 239000002600 sunflower oil Substances 0.000 description 1
- 239000013077 target material Substances 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y403/00—Carbon-nitrogen lyases (4.3)
- C12Y403/03—Amine-lyases (4.3.3)
- C12Y403/03007—4-Hydroxy-tetrahydrodipicolinate synthase (4.3.3.7)
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2013-0121090 | 2013-10-11 | ||
| KR20130121090 | 2013-10-11 | ||
| KR1020140091307A KR101518860B1 (ko) | 2013-10-11 | 2014-07-18 | L-아미노산의 생산 방법 |
| KR10-2014-0091307 | 2014-07-18 | ||
| PCT/KR2014/009468 WO2015053552A1 (en) | 2013-10-11 | 2014-10-08 | Method of producing l-amino acids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2925659A1 CA2925659A1 (en) | 2015-04-16 |
| CA2925659C true CA2925659C (en) | 2018-10-23 |
Family
ID=53035683
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2925659A Active CA2925659C (en) | 2013-10-11 | 2014-10-08 | Method of producing l-amino acids |
Country Status (16)
| Country | Link |
|---|---|
| JP (3) | JP6291042B2 (ru) |
| KR (1) | KR101518860B1 (ru) |
| CN (1) | CN104561163B (ru) |
| AR (1) | AR097977A1 (ru) |
| AU (2) | AU2014332732B2 (ru) |
| BR (1) | BR112016008029A2 (ru) |
| CA (1) | CA2925659C (ru) |
| DK (1) | DK2860256T3 (ru) |
| ES (1) | ES2626048T3 (ru) |
| GE (1) | GEP20196943B (ru) |
| JO (1) | JO3255B1 (ru) |
| MY (1) | MY172463A (ru) |
| PH (1) | PH12016500640A1 (ru) |
| PL (1) | PL2860256T3 (ru) |
| RU (2) | RU2651461C2 (ru) |
| UA (1) | UA117935C2 (ru) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101518860B1 (ko) * | 2013-10-11 | 2015-05-12 | 씨제이제일제당 (주) | L-아미노산의 생산 방법 |
| CN105734004B (zh) * | 2016-03-02 | 2020-06-19 | 廊坊梅花生物技术开发有限公司 | 重组菌株及其制备方法、应用 |
| KR101925930B1 (ko) * | 2017-07-24 | 2019-02-26 | 씨제이제일제당 주식회사 | Ｌ-라이신을 생산하는 미생물 및 이를 이용한 ｌ-라이신의 생산 방법 |
| SG11202002185SA (en) * | 2018-03-09 | 2020-04-29 | Cj Cheiljedang Corp | Novel promoter and l-amino acid production method using same |
| KR102221040B1 (ko) * | 2019-05-09 | 2021-03-03 | 씨제이제일제당 주식회사 | L-아미노산을 생산하는 미생물 및 이를 이용한 l-아미노산을 생산하는 방법 |
| KR102134418B1 (ko) * | 2019-06-17 | 2020-07-16 | 씨제이제일제당 주식회사 | L-타이로신을 생산하는 미생물 및 이를 이용한 l-타이로신 생산 방법 |
| KR102207867B1 (ko) * | 2020-01-21 | 2021-01-26 | 씨제이제일제당 주식회사 | Nadp 의존적 글리세르알데하이드-3-포스페이트 디하이드로지나제를 포함하는 미생물을 이용하여 ｌ-아미노산을 생산하는 방법 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6649379B1 (en) * | 1987-06-12 | 2003-11-18 | Massachusetts Institute Of Technology | Method and deregulated enzyme for threonine production |
| JP3036912B2 (ja) * | 1991-09-02 | 2000-04-24 | 協和醗酵工業株式会社 | 遺伝子発現調節ｄｎａ |
| WO1995023864A1 (fr) * | 1994-03-04 | 1995-09-08 | Ajinomoto Co., Inc. | Procede de production de l-lysine |
| BR9606379A (pt) * | 1995-06-07 | 1997-08-12 | Ajinomoto Kk | Dna recombinante replicavel em células de bactérias corinformes bactérias corineformes processo para produzir l-lisina |
| ATE263246T1 (de) * | 1999-02-20 | 2004-04-15 | Degussa | Verfahren zur fermentativen herstellung von l- aminosäuren unter verwendung coryneformer bakterien |
| EP1241246B1 (en) * | 1999-12-24 | 2008-12-10 | Ajinomoto Co., Inc. | Process for producing l-amino acid |
| WO2002040679A2 (en) * | 2000-11-15 | 2002-05-23 | Archer-Daniels-Midland Company | Corynebacterium glutamicum promoters |
| KR100397423B1 (ko) * | 2001-02-13 | 2003-09-13 | 씨제이 주식회사 | Ｌ-쓰레오닌의 제조방법 |
| DE10224088A1 (de) * | 2002-05-31 | 2003-12-11 | Degussa | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung coryneformer Bakterien die ein abgeschwächtes mez-Gen enthalten |
| JP2004261150A (ja) * | 2003-03-04 | 2004-09-24 | Ajinomoto Co Inc | 目的物質の製造法 |
| JP2005006564A (ja) | 2003-06-19 | 2005-01-13 | Japan Tobacco Inc | 硝酸イオン誘導性プロモーター |
| JP2010226956A (ja) * | 2007-07-23 | 2010-10-14 | Ajinomoto Co Inc | Ｌ−リジンの製造法 |
| KR101335853B1 (ko) | 2011-12-01 | 2013-12-02 | 씨제이제일제당 (주) | Ｌ-아미노산 및 리보플라빈을 동시에 생산하는 미생물 및 이를 이용한 ｌ-아미노산 및 리보플라빈을 생산하는 방법 |
| KR101518860B1 (ko) * | 2013-10-11 | 2015-05-12 | 씨제이제일제당 (주) | L-아미노산의 생산 방법 |
-
2014
- 2014-07-18 KR KR1020140091307A patent/KR101518860B1/ko active IP Right Grant
- 2014-08-10 UA UAA201603717A patent/UA117935C2/uk unknown
- 2014-09-24 DK DK14186238.3T patent/DK2860256T3/en active
- 2014-09-24 PL PL14186238T patent/PL2860256T3/pl unknown
- 2014-09-24 ES ES14186238.3T patent/ES2626048T3/es active Active
- 2014-10-08 BR BR112016008029A patent/BR112016008029A2/pt not_active Application Discontinuation
- 2014-10-08 CA CA2925659A patent/CA2925659C/en active Active
- 2014-10-08 JP JP2016521791A patent/JP6291042B2/ja active Active
- 2014-10-08 RU RU2016110804A patent/RU2651461C2/ru active
- 2014-10-08 RU RU2015151124A patent/RU2614258C2/ru active
- 2014-10-08 MY MYPI2016701087A patent/MY172463A/en unknown
- 2014-10-08 AU AU2014332732A patent/AU2014332732B2/en active Active
- 2014-10-08 GE GEAP201414125A patent/GEP20196943B/en unknown
- 2014-10-09 JO JOP/2014/0287A patent/JO3255B1/ar active
- 2014-10-09 AR ARP140103766A patent/AR097977A1/es unknown
- 2014-10-11 CN CN201410654216.XA patent/CN104561163B/zh active Active
-
2016
- 2016-04-07 PH PH12016500640A patent/PH12016500640A1/en unknown
-
2017
- 2017-12-07 AU AU2017272295A patent/AU2017272295B2/en active Active
-
2018
- 2018-02-08 JP JP2018021175A patent/JP6824918B2/ja active Active
-
2020
- 2020-01-24 JP JP2020009563A patent/JP2020078312A/ja not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| BR112016008029A2 (pt) | 2017-10-03 |
| KR20150042692A (ko) | 2015-04-21 |
| MY172463A (en) | 2019-11-26 |
| KR101518860B1 (ko) | 2015-05-12 |
| CA2925659A1 (en) | 2015-04-16 |
| JP2018113966A (ja) | 2018-07-26 |
| AU2017272295B2 (en) | 2019-05-16 |
| ES2626048T3 (es) | 2017-07-21 |
| DK2860256T3 (en) | 2017-06-06 |
| JO3255B1 (ar) | 2018-09-16 |
| JP6291042B2 (ja) | 2018-03-14 |
| RU2651461C2 (ru) | 2018-04-19 |
| RU2015151124A (ru) | 2016-06-27 |
| PH12016500640B1 (en) | 2016-05-30 |
| AU2014332732B2 (en) | 2017-12-07 |
| PH12016500640A1 (en) | 2016-05-30 |
| JP6824918B2 (ja) | 2021-02-03 |
| CN104561163A (zh) | 2015-04-29 |
| RU2614258C2 (ru) | 2017-03-24 |
| AU2017272295A1 (en) | 2018-01-04 |
| JP2016532439A (ja) | 2016-10-20 |
| UA117935C2 (uk) | 2018-10-25 |
| AU2014332732A1 (en) | 2016-04-21 |
| GEP20196943B (en) | 2019-02-11 |
| RU2016110804A (ru) | 2016-07-27 |
| PL2860256T3 (pl) | 2017-08-31 |
| AR097977A1 (es) | 2016-04-20 |
| CN104561163B (zh) | 2018-11-06 |
| JP2020078312A (ja) | 2020-05-28 |
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